Tissue crusher suitable for primary cell extraction

Abstract

The utility model relates to the technical field of tissue primary cell separation, in particular to a tissue crusher suitable for primary cell extraction, which consists of a cup cover and a cup body, the crushing cavity is composed of an inverted-truncated-cone-shaped guide hopper, an iris opening and closing structure, an outer gear and an outer baffle. The liquid collecting cavity is formed in the bottom of the crushing cavity and used for filtering the crushed fresh / frozen tissue and collecting a cell solution, a protruding part and a filter cup are arranged on the inner side of the upper end of the liquid collecting cavity, and the filter cup is in lap joint with the upper end of the protruding part. According to the tissue crusher provided by the utility model, the crushing time of fresh / frozen tissues is effectively shortened, the activity of subsequently extracted primary cells is further ensured, and the utilization rate of tissue samples is improved; in addition, by arranging the iris opening and closing structure, tissue blocks after enzyme digestion can directly enter the liquid collecting cavity from the crushing cavity, and interference of the external environment is avoided.

Description

Technical Field

[0001] The utility model relates to the technical field of tissue primary cell separation, in particular to a tissue crusher suitable for primary cell extraction. Background Art

[0002] In biological research, cell experiments are an indispensable part of the content, among which the experimental results related to primary cells are considered to have higher credibility and reliability. Common primary cells are obtained from tissue samples of animals, plants or humans. During the acquisition process, the obtained tissue needs to be chopped as much as possible, and then the primary cells are extracted after enzyme digestion. However, it is very difficult to obtain tissue samples. The extraction efficiency of primary cells must be improved as much as possible to make full use of tissue samples. Since primary cells are very fragile, the commonly used tissue fragmentation method is to use ophthalmic scissors to fragment the tissue, but this method is very time-consuming and easy to affect the activity of primary cells. Moreover, the degree of tissue fragmentation is not easy to control. The fragmented tissue blocks are prone to be of different sizes, wasting precious tissue samples. Secondly, a closed space needs to be provided separately during the enzyme digestion process. After the enzyme digestion is completed, the tissue blocks need to be taken out and filtered. During the removal process, the fresh / frozen tissue after enzyme digestion is easily disturbed by the external environment, thereby affecting the activity of primary cells. Utility Model Content

[0003] The utility model provides a tissue crusher suitable for primary cell extraction to solve the technical problems raised in the above background technology.

[0004] The utility model provides a tissue crusher suitable for primary cell extraction, which is composed of a cup cover and a cup body, wherein a crushing device, a crushing chamber and a liquid collecting chamber are arranged in the cup body, wherein:

[0005] The crushing device comprises a mounting cylinder and a plurality of blade groups, wherein the upper end of the mounting cylinder is connected to the lower end of the rotating handle, and each blade group comprises four blades and are arranged at equal intervals radially along the outer wall of the mounting cylinder;

[0006] The crushing chamber is composed of an inverted truncated cone-shaped guide hopper, an iris opening and closing structure, an external gear and an external baffle. The upper end of the inverted truncated cone-shaped guide hopper abuts against the inner wall of the cup body, the outer baffle abuts against the inner wall of the cup body and a second through hole is provided at the center of the outer baffle, and the lower end of the inverted truncated cone-shaped guide hopper abuts against the second through hole; the interior of the outer baffle is a hollow cavity, the iris opening and closing structure is arranged in the outer baffle, the iris opening and closing structure includes an iris rotating ring and an iris gear assembly, the inner surface of the iris rotating ring is provided with a first rack portion, and the outer surface is provided with a second rack portion, the iris gear assembly is composed of a plurality of iris gear opening and closing units symmetrically distributed with the iris rotating ring as the center, and the diameter of the iris gear assembly is The diameter of the iris gear opening and closing unit is equal to that of the second through hole; the iris gear opening and closing unit includes an iris gear and an iris opening and closing blade, and the iris gear is meshed with the first rack portion; a plurality of connecting columns are evenly distributed on the upper end surface of the iris rotating ring, and the connecting columns position and nest the iris outer ring and the iris rotating ring into one body, and a gear baffle is arranged between the iris outer ring and the iris rotating ring; a rotating shaft is arranged at the center of the outer gear, and the rotating shaft is arranged in the outer wall of the cup body; a defective portion is also arranged at one end of the outer baffle plate close to the outer gear, and one end of the outer gear is meshed with the second rack portion in the defective portion, and when the outer gear rotates, the iris rotating ring is driven to rotate in the outer baffle plate, thereby opening / closing the iris opening and closing structure;

[0007] The liquid collecting chamber is arranged at the bottom of the crushing chamber, and is used to filter the crushed fresh / frozen tissue and collect the cell solution. A protrusion and a filter cup are arranged on the inner side of the upper end of the liquid collecting chamber, wherein the filter cup is overlapped on the upper end of the protrusion.

[0008] Furthermore, a rotating handle and a first through hole are provided at the top of the cup cover, an anti-slip cover is provided at one end of the rotating handle outside the cup cover, a sealing ring is provided at the first through hole, and the other end of the rotating handle passes through the cup cover from the first through hole and enters the cup body.

[0009] Furthermore, the cup cover and the cup body are cylindrical, and the cup cover and the cup body are made of high temperature resistant transparent material.

[0010] Furthermore, the diameter of the lower end of the inverted frustum-shaped guide hopper is ≤ 1 / 2 of the diameter of the upper end of the inverted frustum-shaped guide hopper, and an annular scraper is provided at the lower end of the inverted frustum-shaped guide hopper, the diameter of the annular scraper is equal to the diameter of the second through hole, and the bottom of the annular scraper is in contact with the iris gear assembly.

[0011] Furthermore, the length of the blade on the outer wall of the mounting tube decreases successively and is adapted to the size of the inverted frustum-shaped guide hopper, wherein there is a gap between the blade at the bottom of the mounting tube and the iris gear assembly. When the crushing device is working, the blade at the bottom of the mounting tube does not contact the iris gear assembly.

[0012] Furthermore, the fresh / frozen tissue used for crushing does not exceed 1 / 4 of the volume of the crushing chamber.

[0013] Furthermore, the protrusion is an annular protrusion surrounding the inner side of the cup body.

[0014] Furthermore, a limiting ring is provided at the mouth of the filter cup, the limiting ring is overlapped on the upper end of the protrusion, the diameter of the mouth of the filter cup is greater than the diameter of the second through hole, and the mesh diameter of the filter cup is less than 1 mm.

[0015] Furthermore, the first through hole, the mounting tube, the second through hole and the filter cup are coaxially arranged.

[0016] Furthermore, an internal thread is provided on the inner side of the lower end of the rotating handle, and an external thread is provided on the outer side of the upper end of the mounting tube for cooperating with the rotating handle; an internal thread is provided on the inner side of the cup cover, and an external thread is provided on the outer side of the top end of the cup body for cooperating with the cup cover; an internal thread is provided on the inner side of the bottom end of the crushing chamber, and an external thread is provided on the outer side of the top end of the liquid collecting chamber for cooperating with the crushing chamber.

[0017] Compared with the prior art, the utility model has the following beneficial effects:

[0018] 1. Compared with the traditional tissue crushing method, the tissue crusher provided by the utility model effectively shortens the crushing time of fresh / frozen tissues, further ensures the activity of the primary cells extracted subsequently, and improves the utilization rate of tissue samples.

[0019] 2. The utility model provides a crushing device to crush fresh / frozen tissues, which effectively avoids the situation that the crushed tissue blocks are prone to be of different sizes; at the same time, the utility model uses high-temperature resistant transparent materials to make the tissue crusher, which is convenient for the staff to timely grasp the tissue crushing situation during use.

[0020] 3. In the traditional primary cell extraction method, a closed space needs to be provided separately during the enzyme digestion process. After the enzyme digestion is completed, the tissue blocks need to be taken out and filtered. During the removal process, the tissue blocks after enzyme digestion are easily disturbed by the external environment, thereby affecting the activity of the primary cells. The utility model provides a closed space for the enzyme digestion process by setting an iris opening and closing structure, and after the enzyme digestion is completed, the iris opening and closing structure can be opened to allow the tissue blocks after enzyme digestion to directly enter the liquid collection chamber from the crushing chamber, thereby avoiding interference from the external environment.

[0021] 4. The utility model has a simple structural design, a small size, is easy to carry, and has a more reasonable structural layout, which is conducive to mass production. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative labor.

[0023] Figure 1 This is a partial cross-sectional structural schematic diagram of the tissue crusher provided by the utility model;

[0024] Figure 2 A schematic diagram of the iris opening and closing structure provided by the utility model;

[0025] Figure 3 This is a schematic diagram of the installation structure of the external gear and the external baffle provided by the utility model. DETAILED DESCRIPTION

[0026] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0027] In the description of the present utility model, it should be noted that the orientation or position relationship indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inside", "outside", etc. is based on the orientation or position relationship shown in the drawings, which is only for the convenience of describing the present utility model and simplifying the description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation of the present utility model; the terms "first", "second", and "third" are only used for descriptive purposes, and cannot be understood as indicating or implying relative importance. In addition, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present utility model can be understood according to specific circumstances.

[0028] like Figure 1-3 As shown, the utility model provides a tissue crusher suitable for primary cell extraction, which consists of a cup cover 1 and a cup body 2, wherein a crushing device 3, a crushing chamber 4 and a liquid collecting chamber 5 are arranged in the cup body 2.

[0029] The cup cover 1 involved in the utility model is provided with a rotating handle 11 and a first through hole at the top end, the rotating handle 11 is provided with an anti-slip cover 13 at one end outside the cup cover 1, a sealing ring 12 is provided at the first through hole, and the other end of the rotating handle 11 passes through the cup cover 1 from the first through hole and enters the cup body 2.

[0030] By adopting the above technical solution, it is convenient for the user to hold the handle 11 tightly during the hand-cranking process to avoid slipping of the hand, which prolongs the crushing time and thus affects the activity of the primary cells; at the same time, the provision of the sealing ring 12 is conducive to improving the sealing performance in the crushing chamber 4, thereby avoiding the problem of poor separation of tissue blocks due to low sealing performance during enzymatic digestion of tissue blocks.

[0031] It should be noted that, considering that a too fast cutting speed may damage cells and affect the subsequent extraction efficiency of primary cells, the present invention crushes fresh / frozen tissues by manually rotating the handle 11. In addition, a low-speed motor can be used instead of the rotating handle to crush fresh / frozen tissues.

[0032] Furthermore, the cup cover 1 and the cup body 2 are cylindrical, and the cup cover 1 and the cup body 2 are made of high temperature resistant transparent material.

[0033] By adopting the above technical solution, the user can observe the entire crushing process and adjust the speed of the rotating handle 11 in time to avoid insufficient or excessive crushing, thereby determining the optimal crushing state of fresh / frozen tissue.

[0034] The crushing device 3 of the utility model comprises a mounting tube 31 and a plurality of blade groups 32. The upper end of the mounting tube 31 is connected to the lower end of the rotating handle 11. Each blade group 32 is a four-leaf blade and is arranged at equal intervals along the radial direction of the outer wall of the mounting tube 31.

[0035] Furthermore, an inner thread is provided on the inner side of the lower end of the rotating handle 11 , and an outer thread is provided on the outer side of the upper end of the mounting tube 31 to cooperate with the rotating handle 11 for mounting.

[0036] The crushing chamber 4 involved in the utility model is composed of an inverted truncated cone-shaped guide hopper 41, an iris opening and closing structure 42, an outer gear 43 and an outer baffle plate 44. The upper end of the inverted truncated cone-shaped guide hopper 41 abuts against the inner wall of the cup body 2, the outer baffle plate 44 abuts against the inner wall of the cup body 2 on all sides, and a second through hole is provided at the center of the outer baffle plate 44, and the lower end of the inverted truncated cone-shaped guide hopper 41 abuts against the second through hole; the interior of the outer baffle plate 44 is a hollow cavity, the iris opening and closing structure 42 is arranged in the outer baffle plate 44, the iris opening and closing structure 42 includes an iris rotating ring 421 and an iris gear assembly, the inner surface of the iris rotating ring 421 is provided with a first rack portion 422, and the outer surface is provided with a second rack portion 423, the iris gear assembly is composed of a plurality of iris gear opening and closing units 424 symmetrically distributed with the iris rotating ring 421 as the center, and the diameter of the iris gear assembly is equal to the diameter of the second through hole ; The iris gear opening and closing unit 424 includes an iris gear 425 and an iris opening and closing blade 426, and the iris gear 425 is meshed with the first rack portion 422; the upper end surface of the iris rotating ring 421 is also evenly distributed with a plurality of connecting columns 427, and the connecting columns 427 position the iris outer ring 428 and the iris rotating ring 421 to be nested into one, and a gear baffle 429 is provided between the iris outer ring 428 and the iris rotating ring 421; a rotating shaft 431 is provided at the center of the outer gear 43, and the rotating shaft 431 is provided in the outer wall of the cup body 2, and a defective portion 441 is also provided at one end of the outer baffle 44 close to the outer gear 43, and one end of the outer gear 43 is meshed with the second rack portion 423 in the defective portion 441, and when the outer gear 43 rotates, it drives the iris rotating ring 421 to rotate in the outer baffle 44, thereby opening / closing the iris opening and closing structure 42.

[0037] By adopting the above technical solution, the staff can drive the outer gear 43 to rotate, thereby driving the rotational movement and rotation angle of the iris rotating ring 421. When the iris rotating ring 421 rotates, the iris gear 425 drives the iris opening and closing blades 426 to rotate, thereby realizing the opening or closing of the iris opening and closing structure 42. When the tissue block enzymatic digestion process is completed, the staff can drive the outer gear 43 to open the iris opening and closing structure 42, so that the tissue block can quickly enter the liquid collection chamber 5; at the same time, the setting of the iris opening and closing structure 42 is conducive to providing a closed space for the enzymatic digestion process, and after the enzymatic digestion is completed, the iris opening and closing structure 42 can be opened to allow the tissue block to directly enter the liquid collection chamber 5 from the crushing chamber 4, thereby avoiding interference from the external environment.

[0038] Furthermore, the diameter of the lower end of the inverted truncated cone shaped material guide hopper 41 is ≤ 1 / 2 of the diameter of the upper end of the inverted truncated cone shaped material guide hopper 41 .

[0039] Furthermore, the length of the blade 32 on the outer wall of the mounting tube 31 is gradually reduced and adapted to the size of the inverted frustum-shaped guide hopper 41, wherein there is a gap between the blade 32 at the bottom of the mounting tube 31 and the iris gear assembly. When the crushing device 3 is working, the blade 32 at the bottom of the mounting tube 31 does not contact the iris gear assembly.

[0040] Furthermore, an annular scraper 411 is provided at the lower end of the inverted truncated cone-shaped guide hopper 41 . The diameter of the annular scraper 411 is equal to the diameter of the second through hole, and the bottom of the annular scraper 411 abuts against the iris gear assembly.

[0041] By adopting the above technical solution, it is possible to effectively prevent the tissue blocks that fall on the iris gear assembly from being brought into the iris opening and closing structure 42 as the iris gear assembly opens and closes, thereby affecting the subsequent cleaning of the tissue crusher.

[0042] Furthermore, the fresh / frozen tissue used for crushing does not exceed 1 / 4 of the volume of the crushing chamber 4.

[0043] The liquid collecting chamber 5 involved in the utility model is arranged at the bottom of the crushing chamber 4, and is used to filter the crushed fresh / frozen tissue and collect the cell solution. A protrusion 51 and a filter cup 52 are arranged on the inner side of the upper end of the liquid collecting chamber 5, wherein the filter cup 52 is overlapped on the upper end of the protrusion 51.

[0044] Furthermore, the protrusion 51 is an annular protrusion surrounding the inner side of the cup body 2 .

[0045] Furthermore, a limiting ring 521 is provided at the mouth of the filter cup 52, and the limiting ring 521 overlaps the upper end of the protrusion 51. The diameter of the mouth of the filter cup 52 is greater than the diameter of the second through hole, and the mesh diameter of the filter cup 52 is less than 1 mm.

[0046] Furthermore, the first through hole, the mounting cylinder 31 , the second through hole and the filter cup 52 are coaxially arranged.

[0047] Furthermore, the cup cover 1 has an internal thread on the inside, and the top outer side of the cup body 2 has an external thread that cooperates with the cup cover 1 ; the bottom inner side of the crushing chamber 4 has an internal thread, and the top outer side of the liquid collecting chamber 5 has an external thread that cooperates with the crushing chamber 4 .

[0048] By adopting the above technical solution, when cleaning is needed, the cup cover 1, the crushing chamber 4 and the liquid collecting chamber 5 can be separated and cleaned separately, so that the cleaning is cleaner.

[0049] Working principle of the utility model: the tissue crusher needs to be used in a sterile environment. Before using, the staff first unscrews the cup cover 1, then puts the fresh / frozen tissue into the crushing chamber 4, and then covers the cup cover 1 and tightens the cup cover 1 and the cup body 2; when in use, the fresh / frozen tissue is crushed by hand-cranking the handle 11, and the crushing status of the fresh / frozen tissue in the crushing chamber 4 is checked at any time. When the crushed tissue block reaches the most suitable state, the cup cover 1 is unscrewed and the crushing device 3 is taken out, and then pancreatin or collagenase is added for digestion. After the enzyme digests the tissue block for 15 minutes, the digestion is stopped using complete culture medium; then, the staff drives the outer gear 43 on one side of the cup body 2 The rotation then drives the iris rotating ring 421 to rotate. When the iris rotating ring 421 rotates, the iris gear 425 meshing with the first rack portion 422 will also rotate accordingly, thereby driving the iris opening and closing blades 426 to rotate, thereby realizing the opening of the iris opening and closing structure 42. After the iris opening and closing structure 42 is opened, the tissue blocks after enzyme digestion enter the filter cup 52, and the filter cup 52 performs solid-liquid separation on the tissue blocks after enzyme digestion. The tissue blocks remain in the filter cup 52, and the cell solution flows along the filter cup 52 into the liquid collecting chamber 5; finally, the staff separates the crushing chamber 4 from the liquid collecting chamber 5, takes out the filter cup 52 at the upper end of the liquid collecting chamber 5, and uses a pipette to suck away the cell solution from the liquid collecting chamber 5.

[0050] In addition, after use, the staff can disassemble and clean the cup cover 1, the crushing device 3, the crushing chamber 4, and the liquid collecting chamber 5 in turn, and disinfect them after cleaning. After the treatment, the cup cover 1, the crushing device 3, the crushing chamber 4 and the liquid collecting chamber 5 can be reassembled in turn for the next use.

[0051] Finally, it should be noted that the above is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A tissue crusher suitable for primary cell extraction, consisting of a cup cover and a cup body, wherein the cup body is provided with a crushing device, a crushing chamber and a liquid collecting chamber, characterized in that: The crushing device comprises a mounting cylinder and a plurality of blade groups, wherein the upper end of the mounting cylinder is connected to the lower end of the rotating handle, and each blade group comprises four blades and are arranged at equal intervals radially along the outer wall of the mounting cylinder; The crushing chamber is composed of an inverted truncated cone-shaped guide hopper, an iris opening and closing structure, an external gear and an external baffle. The upper end of the inverted truncated cone-shaped guide hopper abuts against the inner wall of the cup body, the outer baffle abuts against the inner wall of the cup body and a second through hole is provided at the center of the outer baffle, and the lower end of the inverted truncated cone-shaped guide hopper abuts against the second through hole; the interior of the outer baffle is a hollow cavity, the iris opening and closing structure is arranged in the outer baffle, the iris opening and closing structure includes an iris rotating ring and an iris gear assembly, the inner surface of the iris rotating ring is provided with a first rack portion, and the outer surface is provided with a second rack portion, the iris gear assembly is composed of a plurality of iris gear opening and closing units symmetrically distributed with the iris rotating ring as the center, and the diameter of the iris gear assembly is The diameter of the iris gear opening and closing unit is equal to that of the second through hole; the iris gear opening and closing unit includes an iris gear and an iris opening and closing blade, and the iris gear is meshed with the first rack portion; a plurality of connecting columns are evenly distributed on the upper end surface of the iris rotating ring, and the connecting columns position and nest the iris outer ring and the iris rotating ring into one body, and a gear baffle is arranged between the iris outer ring and the iris rotating ring; a rotating shaft is arranged at the center of the outer gear, and the rotating shaft is arranged in the outer wall of the cup body; a defective portion is also arranged at one end of the outer baffle plate close to the outer gear, and one end of the outer gear is meshed with the second rack portion in the defective portion, and when the outer gear rotates, the iris rotating ring is driven to rotate in the outer baffle plate, thereby opening / closing the iris opening and closing structure; The liquid collecting chamber is arranged at the bottom of the crushing chamber, and is used to filter the crushed fresh / frozen tissue and collect the cell solution. A protrusion and a filter cup are arranged on the inner side of the upper end of the liquid collecting chamber, wherein the filter cup is overlapped on the upper end of the protrusion.

2. A tissue crusher suitable for primary cell extraction according to claim 1, characterized in that: A rotating handle and a first through hole are provided at the top of the cup cover, a non-slip cover is provided at one end of the rotating handle outside the cup cover, a sealing ring is provided at the first through hole, and the other end of the rotating handle passes through the cup cover from the first through hole and enters the cup body.

3. A tissue crusher suitable for primary cell extraction according to claim 2, characterized in that: The cup cover and the cup body are cylindrical, and are made of high temperature resistant transparent material.

4. A tissue crusher suitable for primary cell extraction according to claim 3, characterized in that: The diameter of the lower end of the inverted frustum-shaped guide hopper is ≤ 1 / 2 of the diameter of the upper end of the inverted frustum-shaped guide hopper, and an annular scraper is provided at the lower end of the inverted frustum-shaped guide hopper. The diameter of the annular scraper is equal to the diameter of the second through hole, and the bottom of the annular scraper abuts against the iris gear assembly.

5. A tissue crusher suitable for primary cell extraction according to claim 4, characterized in that: The length of the blades on the outer wall of the mounting tube decreases successively and is adapted to the size of the inverted truncated cone-shaped guide hopper, wherein there is a gap between the blade at the bottom of the mounting tube and the iris gear assembly. When the crushing device is working, the blade at the bottom of the mounting tube does not contact the iris gear assembly.

6. A tissue disruptor suitable for primary cell extraction according to claim 5, characterized in that: The fresh / frozen tissue used for crushing should not exceed 1 / 4 of the crushing chamber volume.

7. A tissue disruptor suitable for primary cell extraction according to claim 6, characterized in that: The protrusion is an annular protrusion surrounding the inner side of the cup body.

8. A tissue disruptor suitable for primary cell extraction according to claim 7, characterized in that: A limiting ring is provided at the mouth of the filter cup, the limiting ring is overlapped on the upper end of the protruding portion, the diameter of the mouth of the filter cup is greater than the diameter of the second through hole, and the mesh diameter of the filter cup is less than 1 mm.

9. A tissue disruptor suitable for primary cell extraction according to claim 8, characterized in that: The first through hole, the mounting cylinder, the second through hole and the filter cup are coaxially arranged.

10. A tissue disruptor suitable for primary cell extraction according to claim 9, characterized in that: An internal thread is provided on the inner side of the lower end of the rotating handle, and an external thread is provided on the outer side of the upper end of the mounting tube to cooperate with the rotating handle; an internal thread is provided on the inner side of the cup cover, and an external thread is provided on the outer side of the top end of the cup body to cooperate with the cup cover; an internal thread is provided on the inner side of the bottom end of the crushing chamber, and an external thread is provided on the outer side of the top end of the liquid collecting chamber to cooperate with the crushing chamber.

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