Nucleic acid extraction-free pipe
The nucleic acid extraction-free tube facilitates efficient and safe nucleic acid extraction by integrating lysis solution and proteinase K within the tube, allowing sample release and discharge without external handling, addressing the limitations of current methods.
Patent Information
- Application Number
- CN202420907262.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-04-28
- Publication Date
- 2024-12-27
- Estimated Expiration
- 2034-04-28
AI Technical Summary
Current nucleic acid extraction methods require immediate use of proteinase K, are cumbersome, and pose risks of cross-infection and operator infection due to sample handling outside the laboratory environment.
A nucleic acid extraction-free tube with a tube cover, tube body, and tail cover, featuring a thin wall that can be pierced by a movable piercing member to connect internal cavities containing lysis solution and proteinase K, allowing sample nucleic acid release within the tube, and a syringe-like structure for safe discharge.
Enables efficient, safe, and reliable nucleic acid extraction within the tube, avoiding external interference and cross-infection, with a simple operation and stable structure ensuring accurate piercing and controlled sample discharge.
Smart Images
Figure CN222250725U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of molecular diagnosis, in particular to a nucleic acid extraction-free tube. Background Art
[0002] Nucleic acid detection technology has great application value in molecular diagnosis, biochemical analysis, and disease diagnosis, especially for the detection of nucleic acids of viruses, bacteria, and pathogens. At present, after the nucleic acid samples are collected, lysate is usually used with the aid of proteinase K for nucleic acid extraction. Since the activity of proteinase K in the lysate decreases over time, it cannot be stored for a long time and needs to be prepared and used immediately. It is often necessary to collect a batch of nucleic acid samples and then transport them to the laboratory environment for extraction. The process is cumbersome and there is also the risk of cross-infection of samples and infection of operators. Therefore, this technical field is in urgent need of a nucleic acid extraction device that is not interfered by the external environment, quickly releases sample nucleic acids, is easy to use, and is efficient. Utility Model Content
[0003] In order to overcome the defects of the prior art, the technical problem to be solved by the utility model is to propose a nucleic acid extraction-free tube, which adopts the following technical solutions:
[0004] A nucleic acid extraction-free tube, characterized in that it comprises a tube cover, a tube body and a tail cover, wherein the tube body is provided with a thin wall inside, and the tube cover and the tail cover are provided at both ends respectively, forming a first cavity and a second cavity inside the tube body;
[0005] The tail cover is provided with a piercing member, and the tail cover is arranged on the tube body to move back and forth between a first position and a second position, and when the tail cover is in the second position, the piercing member pierces the thin wall.
[0006] As a further improvement, it also includes a plug body, and the tail cover is fixedly provided with a plug body inside the second cavity.
[0007] As a further improvement, the tail cover is provided with a boss, the piercing member is arranged on the boss, and the plug body is fixedly sleeved on the outer periphery of the boss.
[0008] As a further improvement, the tail cover is provided with a clamping portion, and the side wall of the tube body is provided with a second limiting portion and a first limiting portion. When the tail cover is in the first position, the clamping portion is clamped to the first limiting portion, and when the tail cover is in the second position, the clamping portion is clamped to the second limiting portion.
[0009] As a further improvement, the tube body is provided with a limiting protrusion, and the tail cover is provided with a limiting groove matching the limiting protrusion.
[0010] As a further improvement, the tube cover is provided with a liquid outlet, and the liquid outlet sleeve is provided with a tube cap.
[0011] As a further improvement, the tube cover is provided with a filter element at the liquid outlet.
[0012] As a further improvement, the tube body is provided with a plurality of swab scratching plates on the inner wall of the first cavity.
[0013] As a further improvement, the cross section of the piercing end of the piercing member is cross-shaped.
[0014] As a further improvement, the outer wall of the tube body is provided with a plurality of anti-slip convex strips.
[0015] As a further improvement, the side walls of the tube cap, tube cover and tail cover are provided with a plurality of anti-slip grooves.
[0016] Compared with the prior art, the beneficial effects of the utility model are:
[0017] First, the utility model includes a first cavity and a second cavity, which respectively contain a lysis solution and a re-dissolving proteinase K powder. After the nucleic acid swab collects the sample, the sample is retained in the first cavity by the swab scraping plate arranged in the first cavity, the tube cover and the tube cap are tightened, and the tail cover is pushed to the second position, so that the puncture member pierces the thin wall to connect the two cavities, the lysis solution is mixed with the re-dissolving proteinase K powder, and the sample nucleic acid is quickly released. The whole process is completed inside the tube body, is not subject to external interference, has a simple structure, is convenient to operate, and is safe and reliable.
[0018] Secondly, the utility model is provided with a plug body on the bottom cover, and the plug body cooperates with the bottom cover to form a syringe structure. By unscrewing the tube cap and pushing the bottom cover, the nucleic acid sample liquid is discharged from the liquid outlet, avoiding cross infection, and the operation is safe and convenient, and the sample volume can also be controlled. Furthermore, the filter element at the liquid outlet can perform coarse filtration on the nucleic acid sample liquid, such as the fragments generated when piercing the thin wall, the cotton scraps left by the swab, etc.
[0019] Thirdly, the utility model enables the tail cover to move between the first position and the second position through the first limiting part and the second limiting part. Before sampling, the tail cover is clamped in the first limiting part and is in the first position. After sampling, the tail cover is pushed to the second position so that the piercing member pierces the thin wall. At this time, the limiting protrusion is clamped in the limiting groove and cannot be pushed further, thus avoiding damage to the tube body caused by excessive pushing and ensuring structural stability. The limiting protrusion and the limiting groove further have a guiding function, so that the piercing member accurately pierces the middle of the thin wall, improving the stability during use. BRIEF DESCRIPTION OF THE DRAWINGS
[0020] In order to more clearly illustrate the technical solution of the implementation mode of the utility model, the following is a brief introduction to the drawings required for use in the implementation mode. It should be understood that the following drawings only show certain embodiments of the utility model and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other related drawings can be obtained based on these drawings without paying creative work.
[0021] Figure 1 It is a schematic diagram of the overall structure of the utility model;
[0022] Figure 2 It is a schematic diagram of the structure of the longitudinal section of the utility model;
[0023] Figure 3 It is an explosion diagram of the utility model;
[0024] Figure 4 It is a structural schematic diagram of the tail cover of the utility model.
[0025] Reference numerals:
[0026] 1-tube cap; 2-tube body; 3-tail cap; 4-plug body; 5-tube cap;
[0027] 11-liquid outlet; 12-filter element;
[0028] 21-first cavity; 22-second cavity; 23-thin wall; 24-first limiting portion; 25-second limiting portion; 26-anti-slip convex strip; 27-limiting convex block;
[0029] 211-Swab scratches the board;
[0030] 31- boss; 32- piercing member; 33- clamping portion; 34- limiting groove. DETAILED DESCRIPTION
[0031] In order to facilitate understanding by those skilled in the art, the structure of the present invention is further described in detail with reference to the accompanying drawings:
[0032] In the description of the present invention, the terms "first" and "second" are used only for descriptive purposes and cannot be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. The orientation or position relationship indicated by the terms "portion", "side", "end", etc. is based on the orientation or position relationship shown in the drawings and is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operate in a specific orientation, and therefore cannot be understood as limiting the present invention.
[0033] like Figure 1As shown, the present application provides a nucleic acid extraction-free tube, comprising a tube cover 1, a tube body 2 and a tail cover 3, wherein the tube cover 1 and the tail cover 3 are respectively arranged at two ends of the tube body 2.
[0034] like Figure 2 In the longitudinal cross-sectional view of the present application shown, the inner wall of the tube body 2 is provided with a destructible thin wall 23, and the above-mentioned tube cover 1 and tube body 2 are respectively provided at both ends, and a first cavity 21 and a second cavity 22 are formed inside the tube body 2 through this structure.
[0035] Combination Figure 1 and Figure 2 The tail cover 3 is provided with a piercing member 32 for destroying the thin wall 23 structure. The tail cover 3 is arranged on the tube body 2 to move back and forth between a first position and a second position, and the piercing member 32 pierces the above-mentioned thin wall 23 when the tail cover 3 is in the second position.
[0036] In a specific embodiment, the tube cover 1 is arranged at the upper end of the tube body 2, the tail cover 3 is arranged at the lower end of the tube body 2, the thin wall 23, the tube body 2 and the tube cover 1 form a first cavity 21, the thin wall 23, the tube body 2 and the tail cover 3 form a second cavity 22, wherein the first cavity 21 is placed in a lysate, and the second cavity 22 is placed in a re-dissolved proteinase K solid powder. Taking nucleic acid collection as an example, after collecting the sample by a nucleic acid swab, the tube cover 1 is opened, the swab is immersed in the lysate in the first chamber, or the swab head is broken and put into the lysate, and the tube cover 1 is reconnected to the top of the tube body 2 after collection for storage. When necessary, the tail cover 3 is moved to the second position, the puncture member 32 destroys the thin wall 23, and the first cavity 21 is connected with the second cavity 22, and then the tail cover 3 is restored to the first position. As the first cavity 21 and the second cavity 22 are connected, the lysate with the sample in the first cavity 21 flows into the second cavity 22 and mixes with the re-dissolved proteinase K solid powder in the second cavity 22. Proteinase K can lyse the virus's coat protein and degrade the histone tightly bound to the nucleic acid, so that the nucleic acid is fully freed into the lysate. Through the above structure and operation method, the lysate and proteinase K are prepared and used in the tube body 2, avoiding the reduction of the activity of proteinase K after the two are mixed for a long time. The whole process is completed inside the tube body 2, free from external interference, and reduces the possibility of cross infection. The structure is simple, the operation is convenient, and it is safe and reliable.
[0037] In another embodiment, the second cavity 22 can also contain a proteinase K solution, and the operation process is the same as the above steps, so that the two liquids can be stored separately to avoid their reaction resulting in a decrease in the activity of the proteinase K.
[0038] like Figure 2 and Figure 3 As shown, the tail cover 3 is fixedly provided with a plug body 4 inside the second cavity 22 .
[0039] Specifically, a boss 31 is provided in the middle of the tail cover 3, the piercing member 32 is provided with the boss 31, the plug body 4 is sleeved on the outer periphery of the boss 31, and the plug body 4 is closely fitted with the inner wall of the second cavity 22 to form a seal. More specifically, the piercing member 32 is a cross-shaped cone integrally formed with the boss 31, and the cross section of the piercing end thereof is cross-shaped. When the tail cover 3 is in the second position, the piercing end of the piercing member 32 pierces the thin wall 23, so that the first cavity 21 is connected with the second cavity 22. In this process, the plug body 4 keeps the second cavity 22 in a sealed state.
[0040] like Figure 4 As shown, the tail cover 3 is provided with a clamping portion 33, and the side wall of the tube body 2 is provided with a second limiting portion 25 and a first limiting portion 24. When the tail cover 3 is in the first position, the clamping portion 33 is clamped to the first limiting portion 24, and when the tail cover 3 is in the second position, the clamping portion 33 is clamped to the second limiting portion 25.
[0041] In a specific embodiment, the clamping portion 33, the first limiting portion 24 and the second limiting portion 25 are all annular boss structures, wherein the upper end faces of the first limiting portion 24 and the second limiting portion 25 are planes and the lower end faces are sloped surfaces, and the clamping portion 33 on the tail cover 3 is opposite to the upper end face, which is a sloped surface and the lower end face is a plane. When the tail cover 3 is in the first position, the clamping portion 33 is limited between the first limiting portion 24 and the second limiting portion 25, and the plane of the clamping portion 33 is in contact with the plane of the first limiting portion 24, and when the tail cover 3 is in the second position, the clamping portion 33 goes over the second limiting portion 25.
[0042] Further, such as Figure 3 and Figure 4 As shown, the tube body 2 is provided with a limiting protrusion 27, and the tail cover 3 is provided with a limiting groove 34 that matches the limiting protrusion 27. When the clamping portion 33 passes over the second limiting portion 25, the limiting groove 34 abuts against the limiting protrusion 27 to prevent further forward pushing, thereby avoiding damage to the tube body 2 due to excessive pushing and ensuring the stability of its structure. Since the plug body 4 is deformable, the limiting groove 34 and the limiting protrusion 27 also have a guiding function, so that the piercing member 32 accurately pierces the middle of the thin wall 23.
[0043] like Figure 1-Figure 3 As shown, the outer wall of the tube body 2 is provided with a plurality of anti-skid convex strips 26, and the side walls of the tube cap 5, the tube cover 1 and the tail cover 3 are provided with a plurality of anti-skid grooves, so as to further improve the stability of holding and using.
[0044] like Figure 2 and Figure 3 As shown, a liquid outlet 11 is provided at the tube cover 1 , a tube cap 5 is sleeved on the liquid outlet 11 , and a filter element 12 is fixedly provided at the liquid outlet 11 .
[0045] like Figure 2As shown, the tube body 2 is provided with a plurality of swab scraping plates 211 on the inner wall of the first cavity 21. After the swab collects the sample, the swab scraping plates 211 allow the collected sample to be fully separated into the lysis solution.
[0046] In a specific embodiment, the tube cover 1 is threadedly connected to the tube body 2, and the upper end of the tube cover 1 is provided with a liquid outlet 11, and the above-mentioned tube cap 5 is threadedly connected to the outside of the liquid outlet 11, and the tube cap 5, the tube cover 1, the tube body 2 and the thin wall 23 form a first cavity 21. Further, when the thin wall 23 is destroyed, the tube cover 1, the tube body 2, the plug body 4 and the tail cover 3 form a syringe structure. After the nucleic acid is sampled and extracted in the tube, the tube cap 5 is unscrewed, the tube body 2 is inverted, so that the liquid outlet 11 is downward, and the tail cover 3 is squeezed from the first position to the second position, and the nucleic acid sample liquid in the tube body 2 is discharged from the liquid outlet 11. After squeezing to the end, the tube body 2 is straightened so that the liquid outlet 11 is upward, and the tail cover 3 is pulled down to reset, and the tube body 2 is inverted again, and the tail cover 3 is squeezed to discharge the nucleic acid sample liquid. Through the above structure, the nucleic acid sample liquid can be discharged without opening the cover, avoiding cross infection, and the operation is safe and convenient. The syringe structure can also control the amount of nucleic acid sample liquid discharged. The filter element 12 fixed at the liquid outlet 11 can perform coarse filtration on the nucleic acid sample liquid, such as filtering out debris generated when the thin wall 23 is punctured, cotton scraps remaining on the swab, and incompletely dissolved proteinase K powder.
[0047] The above description is only the preferred implementation of the utility model, and is not intended to limit the utility model. For those skilled in the art, the utility model can have various modifications and changes. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the utility model shall be included in the protection scope of the utility model.
Claims
1. A nucleic acid extraction-free tube, characterized in that: It comprises a tube cover (1), a tube body (2) and a tail cover (3), wherein a thin wall (23) is provided inside the tube body (2), and the tube cover (1) and the tail cover (3) are provided at both ends respectively, forming a first cavity (21) and a second cavity (22) inside the tube body (2); The tail cover (3) is provided with a piercing member (32), and the tail cover (3) is arranged on the tube body (2) so as to move back and forth between a first position and a second position, and when the tail cover (3) is in the second position, the piercing member (32) pierces the thin wall (23).
2. A nucleic acid extraction-free tube as claimed in claim 1, characterized in that: It also comprises a plug body (4), and the tail cover (3) is fixedly provided with the plug body (4) inside the second cavity (22).
3. The nucleic acid extraction-free tube according to claim 1, characterized in that: The tail cover (3) is provided with a boss (31), the piercing member (32) is arranged on the boss (31), and the plug body (4) is fixedly sleeved on the outer periphery of the boss (31).
4. A nucleic acid extraction-free tube as claimed in claim 1 or 2, characterized in that: The tail cover (3) is provided with a clamping portion (33), and the side wall of the tube body (2) is provided with a second limiting portion (25) and a first limiting portion (24); when the tail cover (3) is in a first position, the clamping portion (33) is clamped to the first limiting portion (24); when the tail cover (3) is in a second position, the clamping portion (33) is clamped to the second limiting portion (25).
5. The nucleic acid extraction-free tube according to claim 3, characterized in that: The tube body (2) is provided with a limiting protrusion (27), and the tail cover (3) is provided with a limiting groove (34) matching the limiting protrusion (27).
6. The nucleic acid extraction-free tube according to claim 1, characterized in that: The tube cover (1) is provided with a liquid outlet (11), and the liquid outlet (11) is sleeved with a tube cap (5).
7. The nucleic acid extraction-free tube according to claim 4, characterized in that: The tube cover (1) is fixedly provided with a filter element (12) at the liquid outlet (11).
8. The nucleic acid extraction-free tube according to claim 1, characterized in that: The tube body (2) is provided with a plurality of swab scratching plates (211) on the inner wall of the first cavity (21).
9. The nucleic acid extraction-free tube according to claim 2, characterized in that: The cross section of the piercing end of the piercing member (32) is in the shape of a cross.
10. The nucleic acid extraction-free tube according to claim 6, characterized in that: The outer wall of the tube body (2) is provided with a plurality of anti-skid convex strips (26), and the side walls of the tube cap (5), the tube cover (1) and the tail cover (3) are provided with a plurality of anti-skid patterns.