Anti-pollution liquid storage bottle suitable for cell sorter

Abstract

The utility model relates to an anti-pollution liquid storage bottle suitable for a cell sorter, which comprises a bottle body, a liquid taking nozzle and a filter tip are arranged at the top of the bottle body, and both the liquid taking nozzle and the filter tip protrude out of the bottle body; a soft rubber cover is mounted on the end face of the liquid taking nozzle; an air filter is installed on the end face of the filter tip and seals the filter tip. The liquid taking assembly comprises a suction pipe which penetrates through the soft rubber cover and extends into the bottle body and a connector pipe which is located outside the bottle body and used for being connected with external equipment. The liquid storage bottle has the effect of reducing the possibility that the buffer solution in the liquid storage bottle is polluted.

Description

Technical Field

[0001] The utility model relates to the field of cell sorting auxiliary tools, in particular to an anti-pollution liquid storage bottle suitable for a cell sorter. Background Art

[0002] When performing cell sorting, washing buffer needs to be added to the sorting tank. Conventional washing buffer used for cell treatment contains a lot of phosphates. If left in the air for a long time, it will breed bacteria, fungi and protozoa, causing the washing buffer to be contaminated. Using contaminated washing buffer will contaminate the sample, and in severe cases, it will cause sample detection failure.

[0003] At present, liquid storage bottles with rubber stoppers are mostly used, and the rubber stoppers are provided with through holes according to the specifications of the cell sorter pump-type liquid adding module. When in use, the silicone tube connected to the pump is inserted into the through hole of the rubber stopper to achieve liquid extraction. However, when the diameter of the silicone tube is exactly the same as the pore diameter of the rubber stopper, the negative pressure formed by the pump pumping out the liquid is not conducive to liquid addition. In this case, the diameter of the hole must be larger than the diameter of the silicone tube. This will cause a gap between the rubber stopper and the silicone tube. The gap will allow the buffer solution in the liquid bottle to directly contact the air, causing contamination. Not only is there a risk of damaging the sample, but also a waste of buffer solution. Although contamination can be prevented by replacing the liquid storage bottle daily, it will increase the tediousness of daily inspection work. Utility Model Content

[0004] Based on the above description, the utility model provides an anti-contamination liquid storage bottle suitable for a cell sorter to solve the problem that the buffer solution in the liquid storage bottle is easily contaminated.

[0005] The utility model solves the above technical problems with the following technical solutions: A contamination-proof liquid storage bottle suitable for a cell sorter comprises a bottle body, a liquid taking nozzle and a filter nozzle are arranged on the top of the bottle body, and the liquid taking nozzle and the filter nozzle both protrude from the bottle body;

[0006] The end surface of the liquid taking nozzle is installed with a soft rubber cover; the end surface of the filter tip is installed with an air filter, and the air filter blocks the filter tip;

[0007] It also includes a liquid collection component, which includes a straw that passes through the soft rubber cover and extends into the bottle body, and an interface tube that is located outside the bottle body and is used for connecting an external device.

[0008] On the basis of the above technical solution, the present invention can also be improved as follows.

[0009] Furthermore, the inner diameter of the liquid taking nozzle gradually increases from any position in the middle to both ends, and the end surface of the liquid taking nozzle extends toward the circumference to form an outer edge, and the soft rubber cover is buckled with the outer edge.

[0010] Furthermore, one end of the straw is fixedly connected to and communicated with the mouthpiece, and the other end is configured as an oblique cone port.

[0011] Furthermore, the interface tube is a curved tube, a bending angle α of the interface tube satisfies 90°≤α≤180°, and an end surface of the interface tube in contact with the soft rubber cover is in contact with a surface of the soft rubber cover.

[0012] Furthermore, the air filter comprises a main filter portion, a portion of which is inserted into the filter tip and a portion of which is in contact with an end surface of the filter tip.

[0013] Furthermore, the straw includes a curved pipe section, the mouthpiece includes a rotating section arranged near one end of the soft rubber cover, the rotating section is rotatably connected to the mouthpiece, and the rotating section is fixedly connected and communicated with the straw.

[0014] Furthermore, the straw also includes straight pipe sections arranged at both ends of the curved pipe section.

[0015] Furthermore, the bottom wall of the bottle body is gradually recessed from the edge to the middle toward the inside of the bottle body.

[0016] Compared with the prior art, the technical solution of this application has the following beneficial technical effects:

[0017] 1. The utility model simultaneously arranges a liquid taking nozzle and a filter nozzle on the top of the bottle body, arranges a soft rubber cover to block the liquid taking nozzle, arranges an air filter to block the filter nozzle, and extends the liquid taking assembly into the bottle body through the liquid taking nozzle, so that when the liquid storage bottle of the application is used to store buffer solution, the buffer solution can be isolated from the outside air, thereby reducing the pollution of the buffer solution by air; at the same time, when the liquid storage bottle of the application is used to take the buffer solution, the air can enter the bottle body through the air filter to reduce the occurrence of negative pressure formed in the bottle body;

[0018] 2. The interface pipe is designed as a curved pipe, so that the port of the pipe is horizontal or facing downward, thereby reducing dust or pollutants from entering the bottle through the pipe port, thereby reducing the contamination of the buffer solution;

[0019] 3. An inclined cone surface is provided at the end of the straight tube section of the straw, which can pierce the soft rubber cover through the inclined cone surface, so that the straw can be inserted into the bottle body, and then the hole opened on the soft rubber cover matches the diameter of the straw, thereby reducing the possibility of contaminants entering the bottle body from the connection between the straw and the soft rubber cover. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 A schematic diagram of the overall structure of an anti-contamination liquid storage bottle suitable for a cell sorter provided in an embodiment of the present application;

[0021] Figure 2A front view of an anti-contamination liquid storage bottle suitable for a cell sorter provided in an embodiment of the present application;

[0022] In the accompanying drawings, the components represented by the reference numerals are listed as follows:

[0023] 1. Bottle body; 2. Liquid collection nozzle; 3. Filter tip; 4. Soft rubber cover; 5. Air filter; 51. Air inlet; 52. Main filter unit; 6. Liquid collection assembly; 61. Straw; 611. Bend section; 612. Straight section; 62. Interface tube; 621. Rotating section. DETAILED DESCRIPTION

[0024] In order to facilitate understanding of the present application, the present application will be described more fully below with reference to the relevant drawings. Embodiments of the present application are provided in the drawings. However, the present application can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the disclosure of the present application more thorough and comprehensive.

[0025] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art to which this application belongs. The terms used herein in the specification of this application are only for the purpose of describing specific embodiments and are not intended to limit this application.

[0026] It will be appreciated that spatial relationship terms such as "under," "beneath," "below," "under," "above," "above," etc., may be used herein to describe the relationship of an element or feature shown in the figures to other elements or features. It will be appreciated that, in addition to the orientations shown in the figures, spatial relationship terms also include different orientations of the device in use and operation. For example, if the device in the accompanying drawings is flipped, an element or feature described as "under other elements" or "under it" or "under it" will be oriented as being "above" the other elements or features. Thus, the exemplary terms "under" and "under" may include both upper and lower orientations. In addition, the device may also include additional orientations (e.g., rotated 90 degrees or other orientations), and the spatial descriptors used herein are interpreted accordingly.

[0027] It should be noted that when an element is considered to be “connected to” another element, it can be directly connected to the other element or connected to the other element through an intervening element.

[0028] When used herein, the singular forms "a", "an", and "said / the" may also include plural forms, unless the context clearly indicates otherwise. It should also be understood that the terms "include / comprise" or "have" etc. specify the presence of stated features, wholes, steps, operations, components, parts or combinations thereof, but do not exclude the possibility of the presence or addition of one or more other features, wholes, steps, operations, components, parts or combinations thereof.

[0029] Reference Figure 1 and Figure 2 The utility model provides a technical solution: an anti-pollution liquid storage bottle suitable for a cell sorter comprises a bottle body 1, which may be a transparent cylindrical glass bottle, and a scale mark is arranged on the surface of the bottle body 1. A liquid taking nozzle 2 and a filter nozzle 3 are arranged on the top of the bottle body 1, and the liquid taking nozzle 2 and the filter nozzle 3 are both protruding from the bottle body 1, wherein the filter nozzle 3 is cylindrical, and the inner diameter of the liquid taking nozzle 2 gradually increases from any position in the middle to both ends.

[0030] Further, see Figure 1 and Figure 2 A soft rubber cover 4 is installed at the port of the liquid taking nozzle 2, and the soft rubber cover 4 blocks the end face of the liquid taking nozzle 2. Specifically, the end face of the liquid taking nozzle 2 away from the bottle body 1 extends to the circumferential side to form an outer edge, and the soft rubber cover 4 is buckled with the outer edge. At the same time, the diameter of the soft rubber cover 4 is reasonably set, so that the soft rubber cover 4 can be tightened to the outside of the liquid taking nozzle 2, and then the liquid taking nozzle 2 is closed by the soft rubber cover 4, reducing the possibility of contaminants entering the bottle body 1 through the liquid taking nozzle 2.

[0031] Reference Figure 1 and Figure 2 , an air filter 5 is installed at the port of the filter 3, so that the outside air can enter the bottle 1 after being filtered by the air filter 5. Specifically, the air filter 5 includes an air inlet 51 and a main filter 52. The main filter 52 is a variable diameter structure, and a filter structure such as a filter membrane is arranged in the main filter 52. The filter structure is a prior art and will not be described in detail here. The air inlet 51 is fixed to one side of the main filter 52. When installing the air filter 5, the end of the main filter 52 away from the air inlet 51 is inserted into the filter 3 until the main filter 52 and the port of the filter 3 are tightly pressed. By pressing the main filter 52 and the port of the filter 3 tightly, the gap between the air filter 5 and the filter 3 can be blocked, reducing the possibility of air or pollutants entering the bottle 1 without passing through the air filter 5.

[0032] Reference Figure 1 and Figure 2A contamination-proof liquid storage bottle suitable for a cell sorter also includes a liquid collection component 6, which includes an interface tube 62 and a pipette 61, wherein the pipette 61 pierces the soft rubber cover 4 and then extends into the bottle body 1, and the interface tube 62 is arranged outside the liquid collection nozzle 2 for connection with external equipment, that is, for connection with the pipette of the cell sorter.

[0033] The liquid storage bottle of the present application is provided with a filter tip 3 and a liquid taking nozzle 2 at the same time, so that when taking liquid using the liquid taking assembly 6, air can enter the bottle body 1 after being filtered through the filter tip 3, and the liquid storage bottle can be smoothly taken out without introducing any contaminating buffer solution. In addition, the provision of the soft rubber cover 4 of the present application, the provision of the main filter part 52, and the provision of the straw 61 piercing the soft rubber cover 4 and then extending into the bottle body 1 can ensure the isolation of the internal and external environments of the bottle body 1 as much as possible, and reduce the possibility of air and pollutants entering the bottle body 1.

[0034] Further, see Figure 1 and Figure 2 The interface tube 62 is configured as a curved tube. Specifically, the portion of the interface tube 62 close to the soft rubber cover 4 is a straight segment, and the end face of the interface tube 62 is in contact with the surface of the soft rubber cover 4. As the interface tube 62 extends, the interface tube 62 gradually bends, and the bending angle α of the interface tube 62 satisfies 90°≤α≤180°, so that the end of the interface tube 62 away from the soft rubber cover 4 is horizontal or downward, so that pollutants in the air are not easily introduced into the bottle body 1 from the interface tube 62.

[0035] Further, refer to Figure 1 and Figure 2 The straw 61 includes a curved tube section 611 and straight tube sections 612 disposed at both ends of the curved tube section 611. The end of the straight tube section 612 near the bottom of the bottle body 1 is formed with an oblique conical port, which fits the bottom of the bottle body 1. The oblique conical port facilitates the straw 61 to pierce the soft rubber cover 4 and extend into the bottle body 1. On the other hand, the end of the straw 61 can better fit the bottom of the bottle body 1, thereby draining the buffer solution in the bottle body 1 as much as possible.

[0036] Specifically, refer to Figure 1 and Figure 2 The liquid taking nozzle 2 is arranged at the center of the bottle body 1, and the curved pipe section 611 of the straw 61 is bent from the center to the edge of the bottle body 1, so that the oblique cone surface at the end of the straw 61 is in contact with the bottom wall of the bottle body 1 near the outer edge; further, the bottom wall of the bottle body 1 is gradually recessed from the edge to the middle toward the inside of the bottle body 1.

[0037] Reference Figure 1 and Figure 2 One end of the mouthpiece 62 close to the soft rubber cover 4 also includes a rotating section 621 , the rotating section 621 is rotatably connected to the straight section of the mouthpiece 62 , and the rotating section 621 is fixedly connected to the straw 61 .

[0038] The operator can drive the straw 61 to rotate as a whole in the bottle body 1 by rotating the rotating section 621. Through the above design, when the buffer solution in the bottle body 1 is discharged to the bottom of the bottle body 1, the buffer solution can be gathered to the edge of the bottom wall of the bottle body 1 along the inclined surface of the bottom wall of the bottle body 1. At this time, the end of the straw 61 is in contact with the edge of the bottom of the bottle body 1, and the buffer solution in the bottle body 1 can be better sucked out; the buffer solution in the bottle body 1 can be further sucked out by rotating the straw 61.

[0039] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A contamination-proof liquid storage bottle suitable for a cell sorter, characterized in that: It comprises a bottle body (1), the top of the bottle body (1) is provided with a liquid taking nozzle (2) and a filter nozzle (3), and the liquid taking nozzle (2) and the filter nozzle (3) both protrude from the bottle body (1); The end surface of the liquid taking nozzle (2) is installed with a soft rubber cover (4); the end surface of the filter tip (3) is installed with an air filter (5), and the air filter (5) blocks the filter tip (3); It also comprises a liquid collection assembly (6), wherein the liquid collection assembly (6) comprises a straw (61) passing through the soft rubber cover (4) and extending into the bottle body (1), and an interface tube (62) located outside the bottle body (1) for connection with an external device.

2. The anti-contamination liquid storage bottle suitable for a cell sorter according to claim 1, characterized in that: The inner diameter of the liquid taking nozzle (2) gradually increases from any position in the middle to both ends, and the end surface of the liquid taking nozzle (2) extends toward the circumference to form an outer edge, and the soft rubber cover (4) is buckled with the outer edge.

3. The anti-contamination liquid storage bottle suitable for a cell sorter according to claim 1, characterized in that: One end of the suction tube (61) is fixedly connected to and communicates with the mouthpiece (62), and the other end is configured as an oblique cone port.

4. The anti-contamination liquid storage bottle suitable for a cell sorter according to claim 1, characterized in that: The mouthpiece (62) is a curved pipe, and the bending angle α of the mouthpiece (62) satisfies 90°≤α≤180°. The end surface of the mouthpiece (62) in contact with the soft rubber cover (4) is in contact with the surface of the soft rubber cover (4).

5. The anti-contamination liquid storage bottle suitable for a cell sorter according to claim 1, characterized in that: The air filter (5) comprises a main filter portion (52), wherein a portion of the main filter portion (52) is inserted into the filter tip (3) and a portion of the main filter portion is in contact with the end surface of the filter tip (3).

6. The anti-contamination liquid storage bottle suitable for a cell sorter according to claim 1, characterized in that: The suction tube (61) comprises a curved tube section (611), and the mouthpiece (62) comprises a rotating section (621) arranged at one end close to the soft rubber cover (4), the rotating section (621) being rotatably connected to the mouthpiece (62), and the rotating section (621) being fixedly connected to and in communication with the suction tube (61).

7. The anti-contamination liquid storage bottle suitable for a cell sorter according to claim 6, characterized in that: The suction pipe (61) further comprises straight pipe sections (612) arranged at both ends of the curved pipe section (611).

8. The anti-contamination liquid storage bottle suitable for a cell sorter according to claim 1, characterized in that: The bottom wall of the bottle body (1) is gradually recessed from the edge to the middle toward the inside of the bottle body (1).

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