Kit for detecting aflatoxin B1

Abstract

The utility model relates to the technical field of detection kits, and particularly provides a kit for detecting aflatoxin B1, which comprises a kit body and a kit cover, a detection test strip is arranged in the kit body and comprises a substrate, a sample pad, a combination pad, a nitrocellulose membrane and a water absorption pad are arranged at the upper end of the substrate, and a T line and a C line are arranged on the nitrocellulose membrane; a sample adding hole and an observation hole are formed in the box cover; filter paper is arranged in the sample adding hole; an inner cavity is formed in the box cover, and a push-pull type sealing cover is arranged in the inner cavity in a matched mode. The box cover is further provided with a transparent housing. The aflatoxin B1 detection test strip can be used for detecting the content of aflatoxin B1 in a sample, and due to the design of the filter paper, the abnormal phenomena that the detection test strip is blocked, the color development is not obvious and the like caused by a to-be-detected sample solution with particulate matters can be avoided; due to the design of the push-pull type sealing cover and the transparent housing, the time for sample adding observation operation of the kit is greatly saved, and the detection efficiency and the detection accuracy are improved.

Description

Technical Field

[0001] The utility model relates to the technical field of detection kits, and in particular to a kit for detecting aflatoxin B1. Background Art

[0002] Aflatoxin B1 is a derivative of dihydrofuranoxanaphthene and is highly carcinogenic and toxic.

[0003] The published Chinese patent, with publication number CN209961783U, discloses a rapid quantitative detection kit for aflatoxin B1, including a box and a test strip inside the box; the box includes a movably connected box body and a box cover, the box cover is provided with a sample addition hole and an observation hole, the sample addition hole is located above the sample pad, the observation hole is located above the nitrocellulose membrane, the box cover is provided with a first groove and a second groove, the sample addition hole and the first groove have the same specifications, the observation hole and the second groove have the same specifications, and also includes a first plug body inserted in the sample addition hole and a second plug body inserted in the observation hole.

[0004] The kit disclosed in the above patent has the following deficiencies:

[0005] 1. Since the foods contaminated by aflatoxin B1 are mainly grains and oils, it is easy for grains and oils to mix in with particles during sample preparation. The above-mentioned test kit does not have the function of filtering particles. During the test, particles are likely to enter the test strips, causing blockage, unclear color development and other abnormal phenomena, thus affecting the accuracy of the test;

[0006] 2. The sample adding hole and observation hole are closed with threaded plugs. Before adding samples and observing, the plugs need to be unscrewed one by one, which is troublesome and affects the detection efficiency. Utility Model Content

[0007] In view of the above-mentioned shortcomings of the prior art, the purpose of the utility model is to provide a kit for detecting aflatoxin B1, which is used to solve the problems that the kit used in the prior art does not have the function of filtering particulate matter and the threaded sealing of the sample addition hole and the observation hole are difficult to operate.

[0008] In order to achieve the above-mentioned purpose and other related purposes, the utility model provides a kit for detecting aflatoxin B1, comprising a box body and a box cover fitted on the upper end of the box body;

[0009] A test strip is arranged in the box body, and the test strip comprises a substrate, a sample pad, a binding pad, a nitrocellulose membrane, and a water-absorbing pad are arranged on the upper end of the substrate, and a T line and a C line are arranged on the nitrocellulose membrane;

[0010] The box cover is provided with a sample loading hole and an observation hole, the sample loading hole corresponds to the top of the sample pad, and the observation hole corresponds to the top of the T-line and C-line areas of the nitrocellulose membrane;

[0011] A filter paper is arranged in the sample addition hole to filter the particles in the sample solution to be tested;

[0012] The box cover is provided with an inner cavity penetrating to the sample addition hole, and a push-pull type cover is matched in the inner cavity, and the cover can be pushed and pulled to close in the sample addition hole and cover the top of the filter paper;

[0013] A transparent cover is also provided on the box cover, and the transparent cover is sealed in the observation hole.

[0014] In one embodiment of the present invention, anti-skid silicone pads are provided at the upper and lower ends of the inner cavity, and the anti-skid silicone pads are used for frictionally contacting the upper and lower end surfaces of the cover.

[0015] In one embodiment of the utility model, the box cover is further provided with a groove penetrating to the sample adding hole, and the groove and the inner cavity are arranged opposite to each other on both sides of the sample adding hole for placing the pushed and pulled sealing cap.

[0016] In an embodiment of the present invention, the length of the cover is greater than the sum of the maximum diameter of the sample loading hole and the length of the groove.

[0017] In one embodiment of the present invention, the sample pad is overlapped on the conjugate pad, the conjugate pad is overlapped on one end of the nitrocellulose membrane, and the absorbent pad is overlapped on the other end of the nitrocellulose membrane.

[0018] In one embodiment of the present invention, the conjugate pad is provided with aflatoxin B antibody labeled with fluorescent microspheres, the T line is embedded with aflatoxin B1 antigen, and the C line is embedded with goat anti-rabbit secondary antibody.

[0019] In an embodiment of the present invention, the substrate is a polyvinyl chloride plastic sheet, the sample pad is an absorbent glass fiber membrane, and the absorbent pad is absorbent paper.

[0020] As described above, the kit for detecting aflatoxin B1 of the utility model has the following beneficial effects:

[0021] 1. By placing filter paper in the sample well, the particles in the sample solution to be tested can be filtered to avoid abnormal phenomena such as blockage and unclear color development caused by the sample solution with particles entering the test strip from the sample well, thereby improving the detection accuracy of food contaminated with aflatoxin B1 and the judgment effect of the test results;

[0022] 2. By matching the push-pull cover in the sample addition hole, foreign matter can be prevented from falling into the sample addition hole, and the push-pull structure is more convenient and quick to use; by matching the transparent cover in the observation hole, the transparent cover can prevent foreign matter from falling into the observation hole while not affecting the observation effect of CT line display; compared with the existing technology, the design of the push-pull cover and the transparent cover greatly saves the time of sample addition observation operation of the test kit and improves the detection efficiency;

[0023] 3. When the reagent kit is not in use, the cover is pulled out of the inner cavity to form a closed state in the sample loading hole. By providing a non-slip silicone pad in the inner cavity for placing the cover, it is possible to prevent the cover from freely sliding down the inner cavity and affecting the sealing effect of the sample loading hole;

[0024] 4. The utility model can be used to detect the content of aflatoxin B1 in samples. The design of the filter paper can avoid abnormal phenomena such as blockage of the test strip and unclear color development caused by the sample solution containing particles; the design of the push-pull cover and the transparent cover shell greatly saves the time of sample addition and observation operation of the test kit; the overall design is reasonable and the structure is compact, which can improve the detection efficiency and accuracy of the aflatoxin B1 content in the sample and has high practicality. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 It is a schematic structural diagram showing the closed state of the sample adding hole of the kit for detecting aflatoxin B1 disclosed in the utility model.

[0026] Figure 2 Display as Figure 1 Enlarged view of point A in the middle.

[0027] Figure 3 It shows a schematic structural diagram of the open state of the sample adding hole of the kit for detecting aflatoxin B1 disclosed in the utility model.

[0028] Figure 4 Display as Figure 3 Enlarged view of point B in the middle.

[0029] Component number description

[0030] Box body 1; box cover 2; sample addition hole 21; observation hole 22; inner cavity 23; groove 24; base plate 3; sample pad 4; binding pad 5; nitrocellulose membrane 6; T line 61; C line 62; water absorption pad 7; filter paper 8; sealing cover 9; transparent cover shell 10; non-slip silicone pad 11. DETAILED DESCRIPTION

[0031] The following is a description of the implementation of the present invention by means of specific embodiments. People familiar with the art can easily understand other advantages and effects of the present invention from the contents disclosed in this specification.

[0032] See also Figures 1 to 4 It should be noted that the structures, proportions, sizes, etc. illustrated in the drawings in this specification are only used to match the contents disclosed in the specification for people familiar with this technology to understand and read, and are not used to limit the limiting conditions for the implementation of the utility model, so they have no substantial technical significance. Any modification of the structure, change in the proportion relationship, or adjustment of the size should still fall within the scope of the technical content disclosed by the utility model without affecting the effects and purposes that can be achieved by the utility model. At the same time, the terms such as "upper", "lower", "left", "right", "middle" and "one" quoted in this specification are only for the convenience of description, and are not used to limit the scope of the implementation of the utility model. The change or adjustment of their relative relationship should also be regarded as the scope of the implementation of the utility model without substantially changing the technical content.

[0033] See also Figure 1-4 The utility model provides a test kit for detecting aflatoxin B1, comprising a box body 1 and a box cover 2 matched with the upper end of the box body 1; a test paper strip is arranged in the box body 1, and the test paper strip comprises a substrate 3, and a sample pad 4, a binding pad 5, a nitrocellulose membrane 6, and a water-absorbing pad 7 are arranged on the upper end of the substrate 3, the sample pad 4 is overlapped on the binding pad 5, the binding pad 5 is overlapped on one end of the nitrocellulose membrane 6, and the water-absorbing pad 7 is overlapped on the other end of the nitrocellulose membrane 6; an aflatoxin B1 antibody marked with fluorescent microspheres is arranged in the binding pad 5, an aflatoxin B1 antigen is embedded on the T line 61, and a sheep anti-rabbit secondary antibody is embedded on the C line 62; the substrate 3 is a polyvinyl chloride plastic sheet, the sample pad 4 is an absorbent glass fiber membrane, and the water-absorbing pad 7 is absorbent paper; a T line 61 and a C line 62 are arranged on the nitrocellulose membrane 6. The box cover 2 is provided with a sample loading hole 21 and an observation hole 22 . The sample loading hole 21 corresponds to directly above the sample pad 4 , and the observation hole 22 corresponds to directly above the area where the T line 61 and the C line 62 are set on the nitrocellulose membrane 6 .

[0034] When in use, the sample is dripped onto the test paper strip through the sample addition hole 21, and the aflatoxin B1 antigen in the sample solution to be tested is combined with the aflatoxin B1 antibody labeled with the fluorescent microspheres in the binding pad 5 and chromatographed forward by capillary action. After reaching the detection area, the aflatoxin B1 antigen fixed on the T line 61 is combined with the remaining unbound aflatoxin B1 antibody labeled with the fluorescent microspheres; the concentration of the aflatoxin B1 antibody labeled with the fluorescent microspheres bound on the T line 61 is inversely proportional to the concentration of aflatoxin B1 in the sample, and the concentration of the fluorescent marker bound to the C line 62 is independent of the concentration of aflatoxin B1 in the sample; the test paper strip is inserted into the test paper strip slot of the fluorescent immunoquantitative POCT analyzer, and the concentration of aflatoxin B1 in the sample solution to be tested can be calculated by using an ID card.

[0035] The sample addition hole 21 is provided with a filter paper 8, which can filter out particulate matter in the sample solution to be tested, and prevent the sample solution to be tested containing particulate matter from entering the test paper strip from the sample addition hole 21 to cause blockage, unclear color development and other abnormal phenomena, thereby improving the detection accuracy of food contaminated with aflatoxin B1 and the judgment effect of the test results.

[0036] The box cover 2 is provided with an inner cavity 23 that passes through the sample loading hole 21, and a push-pull type cover 9 is matched in the inner cavity 23. The cover 9 can be pushed and pulled to be closed in the sample loading hole 21 and blocked above the filter paper 8, which can prevent foreign matter from falling into the sample loading hole 21. At the same time, the push-pull structure is more convenient and quick to use; the box cover 2 is also provided with a groove 24 that passes through the sample loading hole 21, and the groove 24 and the inner cavity 23 are relatively distributed on both sides of the sample loading hole 21, which are used to place the push-pull cover 9; the length of the cover 9 is greater than the sum of the maximum diameter of the sample loading hole 21 and the length of the groove 24, which can prevent the cover 9 from being pulled out of the box cover 2; and the cover 9 is set to be L-shaped, which is convenient for manual grasping of the end of the cover 9; the box cover 2 is also provided with a transparent cover shell 10, which is sealed in the observation hole 22, which can prevent foreign matter from falling into the observation hole 22 while not affecting the observation effect of CT line display. Compared with the prior art, the design of the push-pull cover 9 and the transparent cover shell 10 greatly saves the time of sample addition and observation operation of the reagent kit and improves the detection efficiency.

[0037] The upper and lower ends of the inner cavity 23 are both provided with anti-skid silicone pads 11, which are used to frictionally contact the upper and lower end surfaces of the cover 9; because the cover 9 is pulled out of the inner cavity and forms a closed state in the sample addition hole 21 when the reagent kit is not in use, by providing an anti-skid silicone pad 11 in the inner cavity 23 for placing the cover 9, it is possible to prevent the cover 9 from freely sliding down the inner cavity 23 and affecting the sealing effect of the sample addition hole 21.

[0038] In summary, the utility model can be used to detect the content of aflatoxin B1 in samples. The design of the filter paper 8 can avoid abnormal phenomena such as blockage of the test paper strip and unclear coloring caused by the sample solution to be tested with particles; the design of the push-pull cover 9 and the transparent cover 10 greatly saves the time of sample addition and observation operation of the test kit; the overall design is reasonable and the structure is compact, which can improve the detection efficiency and accuracy of the content of aflatoxin B1 in the sample and has high practicality. Therefore, the utility model effectively overcomes various shortcomings in the prior art and has a high industrial utilization value.

[0039] The above embodiments are merely illustrative of the principles and effects of the present invention, and are not intended to limit the present invention. Anyone familiar with the technology may modify or alter the above embodiments without departing from the spirit and scope of the present invention. Therefore, all equivalent modifications or alterations made by a person of ordinary skill in the art without departing from the spirit and technical concept disclosed in the present invention shall still be covered by the claims of the present invention.

Claims

1. A kit for detecting aflatoxin B1, comprising a box body (1) and a box cover (2) fitted on the upper end of the box body (1); A test strip is arranged in the box body (1), the test strip comprises a base plate (3), a sample pad (4), a binding pad (5), a nitrocellulose membrane (6), and a water absorbent pad (7) are arranged on the upper end of the base plate (3), and a T line (61) and a C line (62) are arranged on the nitrocellulose membrane (6); The box cover (2) is provided with a sample loading hole (21) and an observation hole (22), wherein the sample loading hole (21) corresponds to being located directly above the sample pad (4), and the observation hole (22) corresponds to being located directly above the area where the T line (61) and the C line (62) are set on the nitrocellulose membrane (6); Features: A filter paper (8) is arranged in the sample addition hole (21) for filtering particles in the sample solution to be tested; The box cover (2) is provided with an inner cavity (23) which passes through the sample addition hole (21), and a push-pull cover (9) is provided in the inner cavity (23). The cover (9) can be pushed and pulled to seal the sample addition hole (21) and cover the top of the filter paper (8); A transparent cover shell (10) is also provided on the box cover (2), and the transparent cover shell (10) is sealed in the observation hole (22).

2. The kit for detecting aflatoxin B1 according to claim 1, characterized in that: Anti-skid silicone pads (11) are provided at the upper and lower ends of the inner cavity (23), and the anti-skid silicone pads (11) are used for frictionally contacting the upper and lower end surfaces of the cover (9).

3. The kit for detecting aflatoxin B1 according to claim 1, characterized in that: The box cover (2) is also provided with a groove (24) penetrating to the sample loading hole (21), and the groove (24) and the inner cavity (23) are arranged opposite to each other on both sides of the sample loading hole (21) and are used for accommodating the sealing cover (9) that is pushed and pulled out.

4. The kit for detecting aflatoxin B1 according to claim 3, characterized in that: The length of the sealing cover (9) is greater than the sum of the maximum diameter of the sample addition hole (21) and the length of the groove (24).

5. The kit for detecting aflatoxin B1 according to claim 1, characterized in that: The sample pad (4) is overlapped on the conjugate pad (5), the conjugate pad (5) is overlapped on one end of the nitrocellulose membrane (6), and the water-absorbing pad (7) is overlapped on the other end of the nitrocellulose membrane (6).

6. The kit for detecting aflatoxin B1 according to claim 5, characterized in that: The conjugate pad (5) is provided with aflatoxin B1 antibody labeled with fluorescent microspheres, the T line (61) is embedded with aflatoxin B1 antigen, and the C line (62) is embedded with goat anti-rabbit secondary antibody.

7. The kit for detecting aflatoxin B1 according to claim 6, characterized in that: The substrate (3) is a polyvinyl chloride plastic sheet, the sample pad (4) is a water-absorbent glass fiber membrane, and the water-absorbent pad (7) is water-absorbent paper.

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