TUNEL detection kit with accurate measurement

Abstract

The utility model discloses a TUNEL detection kit with accurate measurement, which comprises a kit bottom frame and a plurality of fixed insertion holes arranged in the top end of the kit bottom frame, the height of each fixed insertion hole is smaller than the thickness of the kit bottom frame, a cylindrical kit is inserted into each fixed insertion hole, and the bottom end of the kit bottom frame is connected with the cylindrical kit. The TUNEL detection kit comprises a cylindrical kit body and a fixed insertion hole, the cylindrical kit body is in interference fit with the fixed insertion hole, a shading inspection device is additionally arranged outside the TUNEL detection kit capable of accurately measuring, the shading inspection device can play a role in shading and protection outside the TUNEL detection kit, the shading inspection device can prevent foreign objects from impacting the detection kit, and the TUNEL detection kit capable of accurately measuring the TUNEL detection kit is simple in structure and convenient to use. And meanwhile, the shading protection device can also play a shading role outside the detection kit, so that the situation that the result accuracy is influenced by fading or inactivation of a cell sample and a fluorescent dye in the TUNEL detection kit due to illumination is avoided.

Description

Technical Field

[0001] The utility model belongs to the technical field related to cell detection kits, and specifically relates to a TUNEL detection kit with accurate measurement. Background Art

[0002] The TUNEL assay kit is a kit for detecting cell apoptosis. The kit is based on the principle of DNA breakage during apoptosis. It uses a special enzyme reaction to label dUTP at the end of the DNA break, and detects the label with a fluorescent dye or enzyme marker to identify apoptotic cells. The TUNEL assay kit is widely used in scientific research and clinical diagnosis to study the mechanism of apoptosis and evaluate the effects of drugs or treatments on cell apoptosis.

[0003] However, when the UNEL detection kit is used for cell apoptosis detection, the cell sample, the corresponding marker enzyme and the fluorescent dye need to be placed in the cylindrical kit in the amount required by the experiment, and then it is necessary to wait for the cell apoptosis reaction. During the experiment, it is necessary to regularly observe the special enzyme reaction in the cylindrical kit to mark dUTP at the end of the DNA break and the color of the fluorescent dye. During the observation process, if the external light is strong and directly shines on the cylindrical kit, the light will act on the special marker enzyme and the fluorescent dye, causing the fluorescent dye to fade or inactivate due to light exposure, affecting the accuracy of the results, thereby affecting the experimental results. Utility Model Content

[0004] The utility model aims to provide a TUNEL detection kit with accurate measurement, so as to solve the problem proposed in the above background technology that during the experiment, it is necessary to regularly observe the special enzyme reaction in the cylindrical kit to mark dUTP and the color of the fluorescent dye at the end of the DNA break. During the observation process, if the external light is strong and directly shines on the cylindrical kit, the light acts on the special marking enzyme and the fluorescent dye, causing the fluorescent dye to fade or become inactivated due to the light exposure, affecting the accuracy of the result.

[0005] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a TUNEL detection kit with accurate measurement, comprising a kit base and a plurality of fixed sockets arranged inside the top of the kit base, and the height of each fixed socket is less than the thickness of the kit base, a cylindrical kit is inserted into each of the fixed sockets, the cylindrical kit and the fixed socket are interference fit, and each of the cylindrical kits is provided with accurate measurement scale lines on the outer wall, and a light shielding inspection device is provided on the outside of the kit base and the cylindrical kit;

[0006] The light-shielding inspection device includes a light-proof protective box, a pick-and-place opening, an opening light-shielding cover, a sealing plug and a light-shielding top cover. The right end of the light-proof protective box is provided with a pick-and-place opening, and the reagent kit base frame and a plurality of cylindrical reagent kits are placed into the interior of the light-proof protective box through the pick-and-place opening. An opening light-shielding cover is provided on the outside of the right end of the pick-and-place opening, and a sealing plug is provided on the left end of the opening light-shielding cover. The sealing plug is inserted into the pick-and-place opening, and a light-shielding top cover is provided on the inside of the top of the light-proof protective box.

[0007] Preferably, the light-shielding inspection device also includes a slotted observation window, a light-shielding plate, a push-pull groove and a polarized observation film. A plurality of slotted observation windows are equidistantly arranged inside the front and rear ends of the light-proof protective box. A light-shielding plate and a polarized observation film are inserted into each of the slotted observation windows. Each of the polarized observation films is close to the inner wall of the light-shielding protective box. The light-shielding plate blocks the outside of the polarized observation film. An inwardly recessed push-pull groove is provided on the outer wall of each of the light-shielding plate and the polarized observation film.

[0008] Preferably, the light-shielding inspection device also includes anti-slip slide strips and anti-slip grooves. Two sets of anti-slip grooves are arranged in the inner walls at the left and right ends of each slotted observation window. Anti-slip slide strips are arranged at the left and right ends of the light-shielding plate and the polarized observation film. The anti-slip slide strips at both ends of the light-shielding plate and the polarized observation film are respectively inserted into the corresponding anti-slip grooves.

[0009] Preferably, the anti-slip slide strip can slide up and down in the anti-slip groove, the sealing plug is made of soft rubber, and after the sealing plug is inserted into the inside of the pick-and-place opening, there is an interference fit between the two, and after the shading plate is fully inserted into the slotted observation window, it can prevent external light from entering the internal space of the opaque protection box.

[0010] Preferably, a threaded barrel opening is provided at the top of the cylindrical reagent box, a sealing baffle ring is provided on the outside of the top of the cylindrical reagent box, and a sealing cover is threadedly sleeved on the outside of the threaded barrel opening.

[0011] Preferably, after the sealing cover is completely tightened onto the outside of the threaded barrel mouth, the bottom end of the sealing cover fits tightly with the top end of the sealing ring, and a sealing gasket is also provided on the inner wall of the top end of the sealing cover, and the bottom of the sealing gasket can fit completely on the outer wall of the top end of the threaded barrel mouth.

[0012] Compared with the prior art, the utility model provides a TUNEL detection kit with accurate measurement, which has the following beneficial effects:

[0013] The utility model adds a shading inspection device to the outside of the TUNEL detection kit with accurate measurement. The shading inspection device can produce shading and protection effects on the outside of the TUNEL detection kit. The shading inspection device can prevent foreign objects from hitting the detection kit, thereby protecting the detection kit. At the same time, the shading protection device can also play a shading role on the outside of the detection kit to prevent the cell sample and the fluorescent dye inside the TUNEL detection kit from being exposed to light and fading or inactivating, which affects the accuracy of the result. When detecting DNA breakage in the process of cell apoptosis, the shading plate on the outside of the corresponding cylindrical test kit can be opened to expose the polarized observation film, and then the experimenter can view the corresponding cylindrical test kit through the polarized observation film under dark light conditions. The polarized observation film can deviate the light so that it cannot directly irradiate the cylindrical test kit, thereby preventing external light from affecting the cell sample and the fluorescent dye inside the cylindrical test kit. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 The utility model is a schematic diagram of the overall three-dimensional structure of a TUNEL detection kit with accurate measurement.

[0015] Figure 2 It is a schematic diagram of the three-dimensional structure of the reagent box base and the cylindrical reagent box of the utility model.

[0016] Figure 3 It is a schematic diagram of the three-dimensional structure of the light-shielding inspection device of the present utility model.

[0017] In the figure: 1. Light-shielding inspection device; 2. Reagent box base; 3. Fixed socket; 4. Cylindrical reagent box; 5. Accurate measuring scale line; 6. Sealing ring; 7. Sealing cover; 8. Threaded barrel mouth; 9. Light-proof protective box; 10. Slotted observation window; 11. Light shielding plate; 12. Push-pull groove; 13. Take-and-place opening; 14. Opening light-shielding cover; 15. Sealing plug; 16. Anti-slip slide strip; 17. Anti-slip slide groove; 18. Polarized observation film; 19. Light-shielding top cover. DETAILED DESCRIPTION

[0018] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0019] The utility model provides Figure 1-3The illustrated embodiment shows a TUNEL detection kit with accurate measurement, comprising a kit base frame 2 and a plurality of fixed sockets 3 arranged inside the top of the kit base frame 2, wherein the height of each fixed socket 3 is less than the thickness of the kit base frame 2, a cylindrical kit 4 is inserted into each fixed socket 3, the cylindrical kit 4 and the fixed socket 3 are interference fit, and an accurate measurement scale line 5 is arranged on the outer wall of each cylindrical kit 4, a threaded barrel opening 8 is arranged at the top of the cylindrical kit 4, a sealing retaining ring 6 is arranged on the outside of the top of the cylindrical kit 4, a sealing cover 7 is threadedly sleeved on the outer thread of the threaded barrel opening 8, and after the sealing cover 7 is fully tightened to the outside of the threaded barrel opening 8, the bottom end of the sealing cover 7 is tightly fitted with the top end of the sealing retaining ring 6, a sealing gasket is also arranged on the inner wall of the top end of the sealing cover 7, and the bottom of the sealing gasket can be completely fitted on the threaded barrel opening 8. On the top outer wall, when using the TUNEL detection kit with precise measurement, first, the kit base frame 2 needs to be placed on the laboratory table, and then an experimental dropper needs to be used to absorb an appropriate amount of cell sample, special enzyme corresponding to the cell sample and fluorescent dye and drop them into the tubular reagent kit 4, and the sample capacity is accurately determined by the precise measurement scale line 5 on the outer wall of the tubular reagent kit 4, so that the experimenter can operate the TUNEL detection kit more accurately. After the sample is put in, the sealing cover 7 needs to be sealed to the outside of the threaded barrel mouth 8, so that the inside of the tubular reagent kit 4 is in a sealed state, and then the bottom end of the tubular reagent kit 4 is inserted into the fixed socket 3 on the kit base frame 2, and then the kit base frame 2 and the tubular reagent kit 4 are placed in the light-shielding inspection device 1 to carry out apoptosis detection experiment.

[0020] like Figure 1 and Figure 3As shown, a light-shielding inspection device 1 is arranged outside the reagent box base 2 and the cylindrical reagent box 4, and the light-shielding inspection device 1 includes a light-proof protective box 9, a pick-and-place opening 13, an open light-shielding cover 14, a sealing plug 15 and a light-shielding top cover 19. The right end of the light-proof protective box 9 is provided with a pick-and-place opening 13, and the reagent box base 2 and multiple cylindrical reagent boxes 4 are placed inside the light-proof protective box 9 through the pick-and-place opening 13. An open light-shielding cover 14 is arranged outside the right end of the pick-and-place opening 13, and a sealing plug 15 is arranged at the left end of the open light-shielding cover 14. The sealing plug 15 is inserted into the pick-and-place opening 13, and a light-shielding top cover 19 is arranged inside the top of the light-proof protective box 9. The light-shielding inspection device 1 also includes a slotted observation window 10, a light-shielding plate 11, a push-pull groove 12 and a polarized light observation film 18. Multiple slotted observation windows 10 are equidistantly arranged inside the front and rear ends of the light-proof protective box 9, and each slotted observation window 10 is inserted with a light-shielding plate 11 and a polarized light observation film 1 8, each polarized light observation film 18 is close to the inner wall of the opaque protection box 9, the shading plate 11 blocks the outside of the polarized light observation film 18, and each shading plate 11 and the outer wall of the polarized light observation film 18 are provided with a push-pull groove 12 that is recessed inward, and the shading inspection device 1 also includes an anti-slip slide 16 and an anti-slip groove 17, and each slotted observation window 10 is provided with two sets of anti-slip grooves 17 on the inner wall at both ends of the left and right sides, and the shading plate 11 and the polarized light observation film 18 are provided with a push-pull groove 12 on both ends of the left and right sides. There are anti-slip slide strips 16, and the anti-slip slide strips 16 at both ends of the shading plate 11 and the polarized observation film 18 are respectively inserted into the corresponding anti-slip slide grooves 17, and the anti-slip slide strips 16 can slide up and down in the anti-slip slide grooves 17. The sealing plug 15 is made of soft rubber. After the sealing plug 15 is inserted into the inside of the pick-and-place opening 13, there is an interference fit between the two. After the shading plate 11 is fully inserted into the inside of the slotted observation window 10, it can prevent external light from entering the internal space of the opaque protection box 9.

[0021] like Figure 1 and Figure 3As shown, after the cell sample and other related reagents are put into the cylindrical reagent box 4, it is necessary to insert the bottom end of the cylindrical reagent box 4 into the fixed socket 3 on the reagent box base 2, and then put the reagent box base 2 and the cylindrical reagent box 4 on the base into the light-proof protective box 9 through the access opening 13 at the right end of the light-proof protective box 9, and then completely insert the sealing plug 15 on the opening light-shielding cover 14 into the access opening 13 until the opening light-shielding cover 14 fits on the outer wall of the right end of the light-proof protective box 9. This will completely protect the outside of the cylindrical reagent box 4 and prevent external light from irradiating into the light-proof protective box 9 and damaging the samples inside the cylindrical reagent box 4. When it is necessary to observe the apoptosis of the cell samples inside the cylindrical reagent box 4 inside the light-proof protective box 9, it is first necessary to move the light-shielding inspection device 1 to a dark light environment, and then find the slotted observation window 10 corresponding to the cylindrical reagent box 4, and then fit the finger on the push-pull groove 12 and push upward through the push-pull groove 12 The shading plate 11 inside the slotted observation window 10 is separated from the slotted observation window 10, and the polarized observation plate 18 inside the slotted observation window 10 is exposed. When light passes through the polarized observation plate 18 in a dark environment, the polarized observation plate 18 will deflect the light, so that the light finally acting on the cylindrical reagent box 4 will not have a destructive effect on the cell sample or the fluorescent dye. Then, the experimenter can observe the fluorescent dye or enzyme labeling detection through a special enzyme reaction at the end mark of the DNA break. Remember dUTP, so as to identify apoptotic cells. After the observation is completed, the shading plate 11 needs to be reinserted into the slotted observation window 10, so as to block the external light outside the light-proof protection box 9 again. At the same time, when the shading plate 11 is inserted into the slotted observation window 10, the anti-slip slide strips 16 at both ends of the shading plate 11 will be inserted into the anti-slip slide groove 17, so as to avoid the shading plate 11 from detaching from the slotted observation window 10, thereby ensuring that the shading plate 11 can always play the role of light shielding protection.

[0022] Finally, it should be noted that the above is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A TUNEL detection kit with accurate measurement, comprising a kit base frame (2) and a plurality of fixed sockets (3) arranged inside the top of the kit base frame (2), wherein the height of each fixed socket (3) is less than the thickness of the kit base frame (2), a cylindrical kit (4) is inserted into each of the fixed sockets (3), the cylindrical kit (4) and the fixed sockets (3) are interference fit, and each of the cylindrical kits (4) is provided with an accurate measurement scale line (5) on the outer wall, characterized in that: The reagent box base frame (2) and the outside of the cylindrical reagent box (4) are provided with a light-shielding inspection device (1); The light-shielding inspection device (1) comprises a light-proof protective box (9), a pick-and-place opening (13), an opening light-shielding cover (14), a sealing plug (15) and a light-shielding top cover (19); the right end of the light-proof protective box (9) is provided with a pick-and-place opening (13); the reagent kit base frame (2) and a plurality of cylindrical reagent kits (4) are placed inside the light-proof protective box (9) through the pick-and-place opening (13); the right end of the pick-and-place opening (13) is provided with an opening light-shielding cover (14); the left end of the opening light-shielding cover (14) is provided with a sealing plug (15); the sealing plug (15) is inserted into the pick-and-place opening (13); and the top of the light-proof protective box (9) is provided with a light-shielding top cover (19).

2. A TUNEL detection kit with accurate measurement according to claim 1, characterized in that: The light-shielding inspection device (1) further comprises a slotted observation window (10), a light-shielding plate (11), a push-pull groove (12) and a polarized observation film (18); a plurality of slotted observation windows (10) are evenly spaced inside the front and rear ends of the light-proof protective box (9); a light-shielding plate (11) and a polarized observation film (18) are inserted into each of the slotted observation windows (10); each of the polarized observation films (18) is close to the inner wall of the light-shielding protective box (9); the light-shielding plate (11) blocks the outside of the polarized observation film (18); and each of the light-shielding plate (11) and the polarized observation film (18) is provided with an inwardly recessed push-pull groove (12) on the outer wall.

3. A TUNEL detection kit with accurate measurement according to claim 2, characterized in that: The light-shielding inspection device (1) further comprises an anti-slipping slide bar (16) and an anti-slipping slide groove (17); two sets of anti-slipping slide grooves (17) are arranged in the inner walls at the left and right ends of each slotted observation window (10); anti-slipping slide bars (16) are arranged at the left and right ends of the light-shielding plate (11) and the polarized light observation film (18); the anti-slipping slide bars (16) at the two ends of the light-shielding plate (11) and the polarized light observation film (18) are respectively inserted into the corresponding anti-slipping slide grooves (17).

4. A TUNEL detection kit with accurate measurement according to claim 3, characterized in that: The anti-slip slide strip (16) can slide up and down in the anti-slip slide groove (17); the sealing plug (15) is made of soft rubber; after the sealing plug (15) is inserted into the interior of the take-in and put-out opening (13), an interference fit is formed between the two; after the shading plate (11) is fully inserted into the interior of the slotted observation window (10), external light can be prevented from entering the internal space of the light-proof protective box (9).

5. A TUNEL detection kit with accurate measurement according to claim 1, characterized in that: The top of the cylindrical reagent box (4) is provided with a threaded barrel opening (8), the outside of the top of the cylindrical reagent box (4) is provided with a sealing ring (6), and the external thread of the threaded barrel opening (8) is sleeved with a sealing cover (7).

6. A TUNEL detection kit with accurate measurement according to claim 5, characterized in that: After the sealing cover (7) is completely screwed onto the outside of the threaded barrel mouth (8), the bottom end of the sealing cover (7) fits tightly against the top end of the sealing retaining ring (6). A sealing gasket is also provided on the inner wall of the top end of the sealing cover (7), and the bottom of the sealing gasket can fit completely against the outer wall of the top end of the threaded barrel mouth (8).

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