Light path microplate analysis device of light absorption microplate reader

Abstract

The utility model discloses a light absorption microplate reader light path microplate analysis device, which relates to the microplate technical field, and comprises a reading box, the left side wall and the right side wall of the cavity of the reading box are symmetrically provided with slide seats, the slide seats are slidingly connected with slide plates, the left side and the right side of the bottom of each slide plate are fixedly connected with a plurality of springs, and the springs are fixedly connected with the left side and the right side of the bottom of each slide plate. The bottoms of the springs are fixedly connected with strip plates, and the tops of the sliding plates and the bottoms of the strip plates are attached to the interiors of the sliding grooves of the sliding seats. The bottom of the sliding plate is fixedly connected with a vertical elastic sheet; the rear side wall of the cavity of the reading box is rotationally connected with a first rotating rod and a second rotating rod, the surface of the first rotating rod is sleeved with a driving gear, and the surface of the second rotating rod is sleeved with a driven gear; the outer end of the second rotating rod penetrates through the rear side wall of the reading box and is fixedly connected with a pressing block. The problem that the reading is interfered by bubbles and light generated by heating the microporous plate in the prior art is solved.

Description

Technical Field

[0001] The utility model relates to the technical field of micro-well plates, in particular to a light absorption micro-well plate analysis device for an enzyme marker light path. Background Art

[0002] The Chinese utility model with publication number CN215493635U proposes a multifunctional microplate enzyme-labeled analyzer, comprising a platform, a groove is provided in the center of the top of the platform, a support is fixedly connected to the top of the groove of the platform, a lifting device is provided on the top of the support, a bottom plate is fixedly connected to the top of the lifting device, a constant temperature plate is provided in the center of the top of the bottom plate, a photoelectric detector is provided on the top of the constant temperature plate, an enzyme-labeled plate support plate is provided on the top of the photoelectric detector, an enzyme-labeled microplate is provided on the top of the enzyme-labeled microplate support plate, a cylinder is provided on the top of the bottom plate and all around the enzyme-labeled microplate, a push rod is provided at the output end of the cylinder, the output end of the push rod is fixedly connected to a limiting plate, and a groove with the same thickness as the enzyme-labeled microplate is provided on the opposite side of the limiting plate, not only can the microplate be fixed, but also the overall movement of the microplate can be controlled by the cylinder, the structure is simple, and a constant temperature plate is provided on the top of the bottom plate to ensure the stability of the temperature of the microplate.

[0003] However, the above practicality still has shortcomings:

[0004] 1. For photometric detection, strong external light directly irradiates the microplate, which will have a negative impact on the reading observation, making the reading high or unstable. The utility model does not provide a light-controllable or relatively dim reading environment.

[0005] 2. The utility model uses a constant temperature plate to ensure the stability of the temperature of the microplate, so that there is a situation where the microplate is heated to generate tiny bubbles at the bottom of the microplate. The bubbles adhere to the side wall or bottom of the microplate and seriously affect the accuracy of the reading. Utility Model Content

[0006] The utility model aims to solve at least one of the technical problems existing in the prior art. To this end, the utility model proposes a light absorption microplate reader optical path microplate analysis device, which solves the problem of bubbles and light interference with readings generated by heating the microplate in the prior art.

[0007] To achieve the above purpose, the utility model proposes a light absorption microplate reader optical path microplate analysis device as a further solution of the utility model:

[0008] A light path microplate analysis device for an optical absorption microplate reader comprises a reading box, wherein the left and right side walls of the cavity of the reading box are symmetrically provided with a slide seat, a slide plate is slidably connected in the slide seat, a plurality of springs are fixedly connected to the left and right sides of the bottom of the slide plate, a strip plate is fixedly connected to the bottom of the spring, the top of the slide plate and the bottom of the strip plate are both fitted in the sliding groove of the slide seat; a vertical elastic sheet is fixedly connected to the bottom of the slide plate;

[0009] The rear side wall of the cavity of the reading box is rotatably connected with a first rotating rod and a second rotating rod respectively, a driving gear is sleeved on the surface of the first rotating rod, and a driven gear is sleeved on the surface of the second rotating rod; the outer end of the second rotating rod passes through the rear side wall of the reading box and is fixedly connected with a pressing block;

[0010] A fixing component for clamping the microplate is fixedly connected to the top of the slide plate, and a light source module is fixedly installed at the center of the inner top wall of the reading box.

[0011] Furthermore, the second rotating rod is rotatably and slidably connected to the rear side wall of the reading box;

[0012] When the pressing block is partially or completely located outside the reading box, the driving gear and the driven gear are meshed, and the driven gear and the elastic sheet are in contact;

[0013] When the pressing block is completely located inside the reading box, the driving gear and the driven gear are not in contact.

[0014] Furthermore, the fixing assembly includes a through hole, the through hole is opened in the center of the slide plate from top to bottom, and the top of the slide plate is evenly fixedly connected with a fixing frame around the through hole;

[0015] One end of the fixing frame is fixedly connected to a fixing rod, the inner end of the fixing rod is fixedly connected to a first clamping plate, the other end of the fixing frame is fixedly provided with a sliding groove, the sliding groove is elastically connected to a second clamping plate; the top of the fixing frame faces the light source module.

[0016] Furthermore, the inner end of the first rotating rod is fixedly connected with a first bevel gear;

[0017] A rotating block is rotatably connected to the center of the bottom wall of the reading box cavity, and a second bevel gear is fixedly connected to the top of the rotating block. The first bevel gear and the second bevel gear are meshed with each other.

[0018] Furthermore, a threaded rod is fixedly connected to the top center of the second bevel gear, a sliding block is threadedly connected to the threaded rod, and a top rod is fixedly connected to the top of the sliding block; a limit plate is fixedly connected to the bottom wall of the reading box cavity to ensure the upward and downward movement of the sliding block.

[0019] Furthermore, a hot air blower is fixedly connected to the side wall of the reading box, and the output end of the hot air blower is connected to the interior of the reading box through a hose.

[0020] Furthermore, a motor is fixedly connected to the outer wall of the reading box, and an output end of the motor passes through the side wall of the reading box and is fixedly connected to the outer end of the first rotating rod.

[0021] Furthermore, the push rod passes through the through hole and is fixedly connected to the bottom of the fixing frame.

[0022] Compared with the prior art, the beneficial effects of the utility model are:

[0023] The utility model is conducive to eliminating interference from external light sources when reading under stable light conditions. At the same time, bubbles generated by heating in the microplate are eliminated by slight shaking while adjusting the horizontal height of the microplate. The measurement data of the utility model improves the accuracy of the data compared to the comparative patent mentioned in the background technology. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 The three-dimensional Figure 1 ;

[0025] Figure 2 The three-dimensional Figure 2 ;

[0026] Figure 3 This is a schematic diagram of the internal structure of the reading box of the utility model;

[0027] Figure 4 This is a schematic diagram of the fixed frame structure of the utility model;

[0028] Figure 5 The slide structure of the utility model is shown in FIG. Figure 1 ;

[0029] Figure 6 This is a schematic diagram of the structure of the height adjustment and fixing frame assembly of the utility model;

[0030] Figure 7 The slide structure of the utility model is shown in FIG. Figure 2 .

[0031] In the figure: 1, reading box; 2, drawer plate; 3, hot air blower; 4, slide plate; 41, telescopic column; 42, through hole; 5, slide seat; 6, light source module; 7, fixed frame; 8, fixed rod; 9, first clamping plate; 10, slide groove; 11, second clamping plate; 12, strip plate; 13, spring; 14, first rotating rod; 15, second rotating rod; 16, driving gear; 17, driven gear; 18, first bevel gear; 19, rotating block; 20, second bevel gear; 21, threaded rod; 22, sliding block; 23, push rod; 24, limit plate; 25, elastic sheet; 26, motor; 27, pressing block. DETAILED DESCRIPTION

[0032] The technical solution of the utility model will be described clearly and completely in conjunction with the embodiments below. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of them. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0033] like Figure 1-7 As shown, a light absorption microplate reader optical path microplate analysis device comprises a reading box 1, wherein the left and right side walls of the cavity of the reading box 1 are symmetrically provided with a slide seat 5, a slide plate 4 is slidably connected in the slide seat 5, a plurality of springs 13 are fixedly connected to the left and right sides of the bottom of the slide plate 4, a strip plate 12 is fixedly connected to the bottom of the spring 13, the top of the slide plate 4 and the bottom of the strip plate 12 are both fitted in the sliding groove of the slide seat 5; a vertical elastic sheet 25 is fixedly connected to the bottom of the slide plate 4;

[0034] The rear side wall of the cavity of the reading box 1 is rotatably connected with a first rotating rod 14 and a second rotating rod 15, respectively. The surface of the first rotating rod 14 is sleeved with a driving gear 16, and the surface of the second rotating rod 15 is sleeved with a driven gear 17; the outer end of the second rotating rod 15 passes through the rear side wall of the reading box 1 and is fixedly connected with a pressing block 27;

[0035] A fixing assembly for clamping the microplate is fixedly connected to the top of the slide plate 4 , and a light source module 6 is fixedly installed at the center of the inner top wall of the reading box 1 .

[0036] Preferably, the second rotating rod 15 is rotatably and slidably connected to the rear side wall of the reading box 1;

[0037] When the pressing block 27 is partially or completely located outside the reading box 1, the driving gear 16 and the driven gear 17 are meshed, and the driven gear 17 and the elastic sheet 25 are in contact;

[0038] When the pressing block 27 is completely located inside the reading box 1 , the driving gear 16 and the driven gear 17 are not in contact.

[0039] Preferably, the fixing assembly includes a through hole 42, the through hole 42 is opened in the center of the slide plate 4 from top to bottom, and the top of the slide plate 4 is evenly fixedly connected with a fixing frame 7 around the through hole 42;

[0040] One end of the fixing frame 7 is fixedly connected to a fixing rod 8, the inner end of the fixing rod 8 is fixedly connected to a first clamping plate 9, the other end of the fixing frame 7 is fixedly provided with a slide groove 10, and the slide groove 10 is elastically connected to a second clamping plate 11; the top of the fixing frame 7 faces the light source module 6.

[0041] Preferably, the inner end of the first rotating rod 14 is fixedly connected with a first bevel gear 18;

[0042] A rotating block 19 is rotatably connected to the center of the bottom wall of the cavity of the reading box 1, and a second bevel gear 20 is fixedly connected to the top of the rotating block 19. The first bevel gear 18 and the second bevel gear 20 are meshed with each other.

[0043] Preferably, a threaded rod 21 is fixedly connected to the top center of the second bevel gear 20, a sliding block 22 is threadedly connected to the threaded rod 21, and a top rod 23 is fixedly connected to the top of the sliding block 22; the bottom wall of the reading box 1 cavity is fixedly connected to a limit plate 24 that ensures the sliding block 22 moves up and down.

[0044] Preferably, a hot air blower 3 is fixedly connected to the side wall of the reading box 1, and an output end of the hot air blower 3 is connected to the interior of the reading box 1 through a hose.

[0045] Preferably, a motor 26 is fixedly connected to the outer wall of the reading box 1 , and an output end of the motor 26 passes through the side wall of the reading box 1 and is fixedly connected to the outer end of the first rotating rod 14 .

[0046] Preferably, the push rod 23 passes through the through hole 42 and is fixedly connected to the bottom of the fixing frame 7 .

[0047] Working principle:

[0048] When in use, the slide plate 4 is pulled out from the slide seat 5 by the draw plate 2, so that the slide plate 4 slides out of the reading box 1, one end of the microplate is clamped in the first clamping plate 9 on the fixed frame 7, and then the second clamping plate 11 is pushed outward to clamp the other end of the microplate in the second clamping plate 11, and the second clamping plate 11 is released. Under the action of elastic force, the second clamping plate 11 shrinks and clamps the microplate;

[0049] Turn on the light source module 6, adjust the air outlet temperature of the hot air blower 3, and evenly heat the microplate. At this time, the reagent in the heated microplate generates bubbles;

[0050] The pressing block 27 is kept completely located outside the reading box 1, so that the driving gear 16 and the driven gear 17 are meshed, and the motor 26 drives the first rotating rod 14 to rotate, thereby rotating the first bevel gear 18, thereby rotating the second bevel gear 20, and then rotating the threaded rod 21. Limited by the limit plate 24, the sliding block 22 can only move up and down, so that the top rod 23 applies force to the fixed frame 7 upward or downward, thereby stretching or compressing the telescopic column 41, and then adjusting the horizontal height of the fixed frame 7; at the same time, the driving gear 16 drives the driven gear 17 to rotate, and then the driven gear 17 moves the elastic sheet 25, and then the slide plate 4 fixed on the top of the spring 13 produces a slight shake, thereby eliminating bubbles; finally, in the stable light environment of the reading box 1 and after eliminating the interference of bubbles, the photoelectric detector provided by the light source module 6 is used to convert the optical signal in the microplate into a corresponding electrical signal;

[0051] The utility model is conducive to eliminating interference from external light sources by reading under stable light conditions. At the same time, the bubbles generated by heating in the microplate are eliminated by slight shaking while adjusting the horizontal height of the microplate. The measurement data of the utility model is more accurate than the comparative patent mentioned in the background technology.

[0052] The above embodiments are only used to illustrate the technical method of the utility model rather than to limit it. Although the utility model is described in detail with reference to the preferred embodiments, ordinary technicians in the field should understand that the technical method of the utility model can be modified or replaced by equivalents without departing from the spirit and scope of the technical method of the utility model.

Claims

1. A light absorption microplate reader optical path microplate analysis device, characterized in that: The reading box (1) comprises a reading box (1), wherein the left and right side walls of the cavity of the reading box (1) are symmetrically provided with a sliding seat (5), a sliding plate (4) is slidably connected in the sliding seat (5), a plurality of springs (13) are fixedly connected to the left and right sides of the bottom of the sliding plate (4), a strip plate (12) is fixedly connected to the bottom of the spring (13), the top of the sliding plate (4) and the bottom of the strip plate (12) are both fitted in the sliding groove of the sliding seat (5); a vertical elastic sheet (25) is fixedly connected to the bottom of the sliding plate (4); The rear side wall of the cavity of the reading box (1) is rotatably connected to a first rotating rod (14) and a second rotating rod (15), the surface of the first rotating rod (14) is sleeved with a driving gear (16), and the surface of the second rotating rod (15) is sleeved with a driven gear (17); the outer end of the second rotating rod (15) passes through the rear side wall of the reading box (1) and is fixedly connected to a pressing block (27); A fixing component for clamping the microplate is fixedly connected to the top of the slide plate (4), and a light source module (6) is fixedly installed at the center of the inner top wall of the reading box (1).

2. The optical path microplate analysis device of the light absorption microplate reader according to claim 1, characterized in that: The second rotating rod (15) is rotatably and slidably connected to the rear side wall of the reading box (1); When the pressing block (27) is partially or completely located outside the reading box (1), the driving gear (16) and the driven gear (17) are meshed, and the driven gear (17) and the elastic sheet (25) are in contact; When the pressing block (27) is completely located inside the reading box (1), the driving gear (16) and the driven gear (17) are not in contact.

3. The optical path microplate analysis device of the light absorption microplate reader according to claim 2, characterized in that: The fixing assembly comprises a through hole (42), the through hole (42) is opened in the center of the slide plate (4) from top to bottom, and a fixing frame (7) is evenly fixedly connected to the top of the slide plate (4) around the through hole (42); One end of the fixed frame (7) is fixedly connected to a fixed rod (8), the inner end of the fixed rod (8) is fixedly connected to a first clamping plate (9), the other end of the fixed frame (7) is fixedly provided with a slide groove (10), and the slide groove (10) is elastically connected to a second clamping plate (11); the top of the fixed frame (7) faces the light source module (6).

4. The optical path microplate analysis device of the light absorption microplate reader according to claim 3, characterized in that: The inner end of the first rotating rod (14) is fixedly connected to a first bevel gear (18); A rotating block (19) is rotatably connected to the center of the bottom wall of the cavity of the reading box (1), and a second bevel gear (20) is fixedly connected to the top of the rotating block (19), and the first bevel gear (18) and the second bevel gear (20) are meshed with each other.

5. The optical path microplate analysis device of the light absorption microplate reader according to claim 4, characterized in that: A threaded rod (21) is fixedly connected to the center of the top of the second bevel gear (20), a sliding block (22) is threadedly connected to the threaded rod (21), and a top rod (23) is fixedly connected to the top of the sliding block (22); a limit plate (24) is fixedly connected to the bottom wall of the cavity of the reading box (1) to ensure the sliding block (22) moves up and down.

6. The optical path microplate analysis device of the light absorption microplate reader according to claim 5, characterized in that: A hot air blower (3) is fixedly connected to the side wall of the reading box (1), and the output end of the hot air blower (3) is connected to the interior of the reading box (1) through a hose.

7. The optical path microplate analysis device of the light absorption microplate reader according to claim 6, characterized in that: The outer wall of the reading box (1) is fixedly connected to a motor (26), and the output end of the motor (26) passes through the side wall of the reading box (1) and is fixedly connected to the outer end of the first rotating rod (14).

8. The optical path microplate analysis device of the light absorption microplate reader according to claim 7, characterized in that: The top rod (23) passes through the through hole (42) and is fixedly connected to the bottom of the fixed frame (7).

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