Blending device for producing CAR (chimeric antigen receptor) molecular exosome
By designing a CAR molecular exosome mixing device including measuring cylinder, piston, stirring system and heat conduction tube, the problem of poor mixing accuracy in the prior art is solved, accurate mixing and uniform stirring of raw materials are achieved, and the product is improved quality.
Patent Information
- Application Number
- CN202421059080.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-05-15
- Publication Date
- 2025-01-14
- Estimated Expiration
- 2034-05-15
AI Technical Summary
The existing CAR molecular exosome mixing devices have poor accuracy during manual mixing, which makes it difficult to guarantee product quality.
A mixing device including a shell, measuring cylinder, piston, screw, lifting assembly, check valve and agitating system is designed. The amount of raw materials is accurately controlled by the scale on the measuring cylinder, and the mixing blade and scraper are used to achieve full stirring and Mix evenly while maintaining the appropriate temperature through the heat conductor tube and heater.
Accurate mixing and uniform stirring of raw materials is achieved, product quality is improved, and the therapeutic effect of CAR molecular exosomes is ensured.
Smart Images
Figure CN222342764U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of CAR molecule exosome production, and specifically to a mixing device for CAR molecule exosome production. Background Art
[0002] CAR molecules, or chimeric antigen receptors, are a key component of CAR-T cell therapy, a type of tumor immunotherapy that introduces CAR molecules into T cells, giving them the ability to target and kill tumor cells.
[0003] In the prior art, in order to better exert the therapeutic effect of CAR molecule exosomes, it is necessary to mix them with different materials in a proportion so that they can play a better role.
[0004] However, most existing mixing devices perform mixing by manual proportioning, which has poor accuracy and makes it difficult to ensure the quality of the product. Utility Model Content
[0005] The purpose of the utility model is to provide a mixing device for producing CAR molecule exosomes to solve the problems raised in the above-mentioned background technology.
[0006] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: including a shell and a measuring cylinder, the lower part of the shell is connected with a discharge port, and the discharge port and the shell are welded, the measuring cylinder is installed on the upper left and right sides of the shell, and the inside of the measuring cylinder is slidably connected with a piston, the upper part of the piston is rotatably connected with a screw rod, and a lifting assembly is installed above the screw rod, the right side of the measuring cylinder is connected with a one-way valve 1, and the one-way valve 1 is welded to the measuring cylinder, the inner side of the shell is installed with a one-way valve 2, and a nozzle is installed below the one-way valve 2, and the above-mentioned arrangement is provided with two left and right groups on the shell, which is convenient for adding different raw materials at the same time.
[0007] Preferably, the lifting assembly includes a motor, a rotating sleeve and a bearing, and a rotating sleeve is installed on the right side of the motor, the lower periphery of the rotating sleeve is wrapped with a bearing, and the rotating sleeve and the screw rod are threadedly connected, and when the rotating sleeve rotates, it drives the screw rod to move upward or downward.
[0008] Preferably, a second motor is installed in the upper middle portion of the housing, and a stirring shaft is installed below the second motor.
[0009] Preferably, the upper periphery of the stirring shaft is wrapped with a stirring rod, and stirring blades are arranged below the stirring rod. During the stirring process, the stirring blades send the raw materials transferred from the bottom to the upper stirring rod for sufficient stirring.
[0010] Preferably, a scraper is installed at the bottom of the stirring shaft, and the scraper is welded to the stirring shaft. The scraper on the stirring shaft is at a certain angle, which can scrape up the raw materials at the bottom of the shell and send them to the top.
[0011] Preferably, a heat conducting pipe is wound inside the side wall of the shell, and a circulation pump is connected to the left side of the heat conducting pipe.
[0012] Preferably, a heater is connected above the circulation pump, and a temperature sensor is connected above the heater, and the heater is controlled by the temperature sensor to maintain a suitable temperature during stirring.
[0013] Compared with the prior art, the utility model has the following beneficial effects: the upward movement of the screw rod drives the piston upward movement at the same time, at which time the second one-way valve is closed, and the added material is sucked from the first one-way valve, and the added amount is accurately controlled by the scale on the measuring cylinder, and the circulating pump drives the heat-conducting liquid to circulate in the heat-conducting tube, and the heat-conducting liquid is heated to a suitable temperature by the heater controlled by the temperature sensor and then transfers the heat to the raw materials inside the shell, the second motor drives the stirring shaft to rotate, and the scraper on the stirring shaft is at a certain angle, so as to scrape up the raw materials at the bottom of the shell and send them to the top, and the stirring blade sends the raw materials transferred from the bottom to the upper stirring rod for sufficient stirring during the stirring process.
[0014] The utility model provides a mixing device for producing CAR molecule exosomes. BRIEF DESCRIPTION OF THE DRAWINGS
[0015] Figure 1 This is a front cross-sectional schematic diagram of the structure of the utility model;
[0016] Figure 2 This is a partial cross-sectional schematic diagram of the front side of the measuring cylinder structure of the utility model;
[0017] Figure 3 It is a three-dimensional schematic diagram of the structural lifting component of the utility model.
[0018] In the figure: 1. housing; 2. measuring cylinder; 3. piston; 4. screw; 5. lifting assembly; 501. motor 1; 502. rotating sleeve; 503. bearing; 6. one-way valve 1; 7. one-way valve 2; 8. nozzle; 9. motor 2; 10. stirring shaft; 11. stirring rod; 12. stirring blade; 13. scraper; 14. circulating pump; 15. heater; 16. temperature sensor; 17. heat pipe; 18. discharge port. DETAILED DESCRIPTION
[0019] In order to make the purpose and technical solution of the utility model clearly and completely described, and the advantages more clearly understood, the embodiments of the utility model are further described in detail in conjunction with the accompanying drawings. It should be understood that the specific embodiments described here are part of the embodiments of the utility model, not all of the embodiments, and are only used to explain the embodiments of the utility model, and are not used to limit the embodiments of the utility model. All other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model. Embodiment 1
[0020] See also Figures 1 to 3 The utility model provides a technical solution: comprising a shell 1 and a measuring cylinder 2, a discharge port 18 is connected to the lower part of the shell 1, and the discharge port 18 is welded to the shell 1, the measuring cylinder 2 is installed on the upper left and right sides of the shell 1, and a piston 3 is slidably connected to the inside of the measuring cylinder 2, a screw rod 4 is rotatably connected to the upper part of the piston 3, and a lifting assembly 5 is installed above the screw rod 4, a check valve 6 is connected to the right side of the measuring cylinder 2, and the check valve 6 is welded to the measuring cylinder 2, a check valve 7 is installed on the left and right sides of the inner part of the shell 1, and a nozzle 8 is installed below the check valve 7, and the lifting assembly 5 includes a motor 50 1. Rotating sleeve 502 and bearing 503, and rotating sleeve 502 is installed on the right side of motor 1 501, and bearing 503 is wrapped around the lower periphery of rotating sleeve 502. Motor 1 501 drives rotating sleeve 502 to rotate in bearing 503, and screw rod 4 moves upward and drives piston 3 to move upward at the same time. At this time, one-way valve 2 7 is closed, and the added material is sucked from one-way valve 1 6. The added amount is accurately controlled by the scale on measuring cylinder 2, and then piston 3 is lowered by motor 1 501, and one-way valve 1 6 is closed. The raw materials are scattered and sprayed to the bottom from nozzle 8 through one-way valve 2 7, which is convenient for mixing various raw materials. Embodiment 2
[0021] On the basis of Example 1, in order to achieve the above, a heat-conducting pipe 17 is wound inside the side wall of the shell 1, and a circulation pump 14 is connected to the left side of the heat-conducting pipe 17, a heater 15 is connected above the circulation pump 14, and a temperature sensor 16 is connected above the heater 15. During the raw material adding process, the circulation pump 14 drives the heat-conducting liquid to circulate in the heat-conducting pipe 17. After the heat-conducting liquid is heated to a suitable temperature by the heater 15 controlled by the temperature sensor 16, the heat is transferred to the raw materials inside the shell 1, thereby preventing the special substances in the raw materials from being inactivated at an inappropriate temperature and ensuring the effect of the product. Embodiment 3
[0022] On the basis of Example 2, in order to achieve, a motor 29 is installed in the upper middle part of the shell 1, and a stirring shaft 10 is installed below the motor 29, the upper outer periphery of the stirring shaft 10 is wrapped with a stirring rod 11, and a stirring blade 12 is arranged below the stirring rod 11, and a scraper 13 is installed at the bottom of the stirring shaft 10, and the scraper 13 and the stirring shaft 10 are welded. When various raw materials are in the shell 1, the motor 29 drives the stirring shaft 10 to rotate, and the scraper 13 on the stirring shaft 10 is at a certain angle, which scrapes up the raw materials at the bottom of the shell 1 and sends them to the top. During the stirring process, the stirring blade 12 sends the raw materials transferred from the bottom to the upper stirring rod 11 for sufficient stirring, and then discharges them through the discharge port 18 after completion, which effectively improves the uniformity of the raw material stirring.
[0023] In actual use, the motor 1 501 drives the rotating sleeve 502 to rotate in the bearing 503, and the screw rod 4 moves upward and drives the piston 3 to move upward at the same time. At this time, the one-way valve 2 7 is closed, and the added material is sucked from the one-way valve 6. The added amount is accurately controlled by the scale on the measuring cylinder 2, and then the piston 3 is lowered by the motor 1 501, and the one-way valve 6 is closed. The raw materials are scattered and sprayed from the nozzle 8 to the bottom through the one-way valve 2 7, which is convenient for mixing various raw materials. During the raw material addition process, the circulating pump 14 drives the heat-conducting liquid to circulate in the heat-conducting pipe 17, and the heat-conducting liquid passes through the temperature transfer tube 17. After the heater 15 controlled by the sensor 16 is heated to a suitable temperature, the heat is transferred to the raw materials inside the shell 1 to prevent the special substances in the raw materials from being inactivated at an inappropriate temperature and to ensure the effect of the product. When various raw materials are in the shell 1, the motor 2 9 drives the stirring shaft 10 to rotate, and the scraper 13 on the stirring shaft 10 is at a certain angle to scrape up the raw materials at the bottom of the shell 1 and send them to the top. During the stirring process, the stirring blade 12 sends the raw materials transferred from the bottom to the upper stirring rod 11 for sufficient stirring. After completion, it is discharged through the discharge port 18, which effectively improves the uniformity of the raw material stirring.
[0024] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.
Claims
1. A mixing device for producing CAR molecule exosomes, comprising a housing (1) and a measuring cylinder (2), characterized in that: The lower part of the housing (1) is connected to a discharge port (18), and the discharge port (18) and the housing (1) are welded. The measuring cylinder (2) is mounted on the upper left and right sides of the housing (1), and a piston (3) is slidably connected to the inside of the measuring cylinder (2). A screw rod (4) is rotatably connected to the upper part of the piston (3), and a lifting assembly (5) is mounted above the screw rod (4). A one-way valve (6) is connected to the right side of the measuring cylinder (2), and the one-way valve (6) and the measuring cylinder (2) are welded. A one-way valve (7) is mounted on the inner left and right sides of the housing (1), and a nozzle (8) is mounted below the one-way valve (7).
2. A mixing device for producing CAR molecule exosomes according to claim 1, characterized in that: The lifting assembly (5) comprises a motor 1 (501), a rotating sleeve (502) and a bearing (503), wherein the rotating sleeve (502) is installed on the right side of the motor 1 (501), and the bearing (503) is wrapped around the lower periphery of the rotating sleeve (502).
3. A mixing device for producing CAR molecule exosomes according to claim 1, characterized in that: A second motor (9) is installed in the middle of the upper part of the housing (1), and a stirring shaft (10) is installed below the second motor (9).
4. A mixing device for producing CAR molecule exosomes according to claim 3, characterized in that: The upper periphery of the stirring shaft (10) is wrapped with a stirring rod (11), and a stirring blade (12) is arranged below the stirring rod (11).
5. A mixing device for producing CAR molecule exosomes according to claim 3, characterized in that: A scraper (13) is installed at the bottom of the stirring shaft (10), and the scraper (13) and the stirring shaft (10) are welded.
6. A mixing device for producing CAR molecule exosomes according to claim 1, characterized in that: A heat conducting pipe (17) is wound inside the side wall of the outer shell (1), and a circulation pump (14) is connected to the left side of the heat conducting pipe (17).
7. A mixing device for producing CAR molecule exosomes according to claim 6, characterized in that: A heater (15) is connected above the circulation pump (14), and a temperature sensor (16) is connected above the heater (15).