Constant temperature mechanism for gene extraction and amplification

Abstract

The utility model relates to the technical field of gene amplification, and discloses a constant temperature mechanism for gene extraction and amplification, which comprises a box body, and a movable seat is arranged in the box body. According to the constant-temperature mechanism for gene extraction and amplification, when a test tube is put into the box body, a locking rod can be upwards screwed out of a threaded hole to unlock the top cover at the top end of the box body, then four groups of compression springs on two sides of the bottom end of a movable seat can rebound, and the movable seat can be upwards jacked to the opening position of the top of the box body; after the top cover is turned over and opened, the test tubes can be quickly inserted into the corresponding test tube slots, when the top cover is closed after placement is finished, the top cover can press the movable seat downwards to the initial position in the box body through the ejector rod, and when the test tubes are taken and the top cover is opened, the test tubes can be driven by the movable seat to automatically extend out of the top end of the box body. The problem that gene sample test tubes are inconvenient to take and place in a constant-temperature mechanism is solved.

Description

Technical Field

[0001] The utility model relates to the technical field of gene amplification, in particular to a constant temperature mechanism for gene extraction and amplification. Background Art

[0002] Gene amplification technology can also be called a cell-free molecular cloning system or a specific DNA sequence in vitro primer-directed enzymatic amplification method. It can specifically amplify extremely small amounts of target DNA by millions of times, thereby greatly improving the analysis and detection capabilities of DNA molecules. When amplifying gene samples, in order to properly preserve the samples, a constant temperature mechanism is required to store the sealed test tubes containing the gene samples.

[0003] However, when the constant temperature mechanism for gene extraction and amplification is used to take and place test tubes containing gene samples, since the test tubes are generally placed at a low position inside the constant temperature mechanism, the taking and placing operations need to be carried out deep inside the constant temperature mechanism, which is not convenient for conveniently taking and placing the gene sample test tubes inside the constant temperature mechanism.

[0004] Now, a new type of constant temperature mechanism for gene extraction and amplification is proposed to solve the above-mentioned shortcomings. Utility Model Content

[0005] The utility model aims to provide a constant temperature mechanism for gene extraction and amplification, so as to solve the problem in the above background technology that it is inconvenient to conveniently take and place the gene sample test tube inside the constant temperature mechanism.

[0006] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a constant temperature mechanism for gene extraction and amplification, comprising a box body, a movable seat is arranged inside the box body, a top cover is arranged on the top of the box body, a mounting plate is fixed to the bottom end of the inside of the box body, and compression springs are respectively fixed to the two ends of the top of the mounting plate, top rods are respectively vertically fixed to the two sides of the top of the movable seat, fixing frames are respectively installed and fixed to the two ends of the left top of the box body, fixing feet are respectively installed and fixed to the two ends of the left side of the top cover, a handle is fixed to the right side of the top cover, a locking rod is arranged on the right side of the top of the top cover, and a threaded hole is opened in the middle position of the right side of the top of the box body.

[0007] Furthermore, the top end of the compression spring is fixedly connected to the bottom end of the movable seat, the push rod is symmetrically arranged with respect to the vertical center line of the movable seat, and a through groove is provided inside the mounting plate.

[0008] Furthermore, the bottom end of the fixed foot is hingedly connected to the inside of the fixed frame, the locking rod passes through the inside of the top cover, and the outside of the locking rod is provided with an external thread.

[0009] Furthermore, guide blocks are respectively installed and fixed at both ends of both sides of the movable seat, and guide grooves are respectively opened at both ends of both sides of the inside of the box.

[0010] Preferably, an outer cover is provided on the left side of the exterior of the box body, and knobs are provided at four corners at both ends of the outer cover, and screw holes are provided inside the four corners at both ends of the box body.

[0011] Furthermore, the knob passes through the interior of the outer cover, an external thread is provided on the exterior of the knob, and reserved grooves are provided at both ends of the left side of the top of the outer cover.

[0012] Preferably, a controller is installed and fixed on the right side of the box, and a front cover is provided on the right side of the controller, connecting feet are fixed at both ends of the top of the front cover, connecting frames are installed and fixed at both ends of the top of the controller, the left side of the connecting foot is hingedly connected to the inside of the connecting frame, and a protruding handle is fixed at the bottom end of the front cover.

[0013] Preferably, feet are respectively installed and fixed at the four corners of the bottom end of the box, an air inlet slot is opened at the top of the front end of the box, a temperature sensor is installed and fixed on the right side of the top end of the box, a through hole is penetrated inside the movable seat, a heating ring assembly is installed and fixed at the bottom end of the inside of the box, a fan is installed and fixed at the bottom end of the right side of the box, and a plurality of groups of test tube slots are evenly spaced inside the top end of the movable seat.

[0014] Compared with the prior art, the utility model has the following beneficial effects: the constant temperature mechanism for gene extraction and amplification not only facilitates the convenient placement of gene sample test tubes inside the constant temperature mechanism, facilitates the protection of the outside of the constant temperature mechanism, but also facilitates the protection of the control components of the constant temperature mechanism from accidental touch;

[0015] (1) By providing a box body, a mounting plate, a compression spring, a movable seat, a fixing frame, a fixing foot, a top cover, a push rod, a locking rod, a handle, a threaded hole, a guide groove and a guide block, when placing a gene sample test tube into the interior of the box body, the locking rod can be first screwed upward from the interior of the threaded hole, and then the top cover can be unlocked at the top of the box body. Then, four groups of compression springs arranged on both sides of the bottom of the movable seat will rebound, and the movable seat can be lifted upward to the opening position at the top of the box body. After flipping open the top cover, the test tube can be quickly inserted into the corresponding test tube slot for placement. When the placement is completed and the top cover is closed, the top cover will use the push rod to press the movable seat down to the initial position inside the box body. When taking the test tube, after opening the top cover, the test tube can be automatically extended from the top of the box body by the movable seat, which is convenient for taking and placing the gene sample test tube inside the constant temperature mechanism;

[0016] (2) By providing a box, screw holes, an outer cover and a knob, when the mechanism is used, the outer cover can be inserted from the left side of the box onto the outside of the box, and then the knobs are respectively used at the four corners of the outer cover to penetrate the inside of the outer cover and screwed into the inside of the screw holes accordingly, so that the outer cover can be fixedly installed on the outside of the box. The outer cover can be used to shield and protect the outside of the mechanism from collision, thereby reducing damage to the outside of the mechanism;

[0017] (3) By providing a box body, a front cover, a controller, a connecting frame and a connecting foot, when the constant temperature mechanism is in use, the constant temperature data inside the mechanism is set on the controller. At the same time, when the controller is in use, a front cover is provided on the right side of the controller. The front cover can assist in covering the outside of the controller to avoid abnormal operation of the mechanism caused by accidental touching of the control operation buttons on the right side of the controller. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 It is a front view cross-sectional structural schematic diagram of the utility model;

[0019] Figure 2 This is a front view structural diagram of a compression spring of the utility model;

[0020] Figure 3 It is a schematic diagram of the structure of the movable seat of the utility model in a top view;

[0021] Figure 4 It is a three-dimensional enlarged structural diagram of the box body of the utility model;

[0022] Figure 5 For this utility model Figure 1 A in the figure is an enlarged structural diagram.

[0023] In the figure: 1. base; 2. box body; 3. screw hole; 4. mounting plate; 5. compression spring; 6. movable seat; 7. fixing frame; 8. fixing foot; 9. top cover; 10. ejector rod; 11. air inlet slot; 12. temperature sensor; 13. locking rod; 14. handle; 15. threaded hole; 16. front cover; 17. controller; 18. test tube slot; 19. through hole; 20. heating ring assembly; 21. fan; 22. guide groove; 23. guide block; 24. outer cover; 25. knob; 26. connecting frame; 27. connecting foot. DETAILED DESCRIPTION

[0024] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0025] Example 1: Please refer to Figure 1-5 A constant temperature mechanism for gene extraction and amplification, comprising a box body 2, a movable seat 6 is arranged inside the box body 2, a top cover 9 is arranged at the top of the box body 2, a mounting plate 4 is fixed to the bottom end of the box body 2, and compression springs 5 ​​are respectively fixed to the two ends of the top of the mounting plate 4, top rods 10 are respectively vertically fixed to the two ends of the top of the movable seat 6, fixing frames 7 are respectively installed and fixed to the two ends of the left top of the box body 2, fixing feet 8 are respectively installed and fixed to the two ends of the left side of the top cover 9, a handle 14 is fixed to the right side of the top cover 9, and the right side of the top of the top cover 9 A locking rod 13 is provided, a threaded hole 15 is provided at the middle position of the right side of the top of the box body 2, feet 1 are respectively installed and fixed at the four corners of the bottom end of the box body 2, an air inlet slot 11 is provided at the top of the front end of the box body 2, a temperature sensor 12 is installed and fixed on the right side of the top of the inside of the box body 2, a through hole 19 is provided inside the movable seat 6, a heating ring assembly 20 is installed and fixed at the bottom end of the inside of the box body 2, a fan 21 is installed and fixed at the bottom end of the right side of the box body 2, and a plurality of groups of test tube slots 18 are evenly spaced inside the top of the movable seat 6;

[0026] The top end of the compression spring 5 is fixedly connected to the bottom end of the movable seat 6, the top rod 10 is symmetrically arranged about the vertical center line of the movable seat 6, a through groove is provided inside the mounting plate 4, the bottom end of the fixed foot 8 is hingedly connected to the inside of the fixed frame 7, the locking rod 13 passes through the inside of the top cover 9, and the outside of the locking rod 13 is provided with an external thread, the two ends of both sides of the movable seat 6 are respectively fixed with guide blocks 23, and the two ends of both sides of the inside of the box body 2 are respectively provided with guide grooves 22;

[0027] Specifically, Figure 1-Figure 4As shown, the temperature sensor 12, the heating ring 20 and the fan 21 inside the constant temperature mechanism are electrically connected to the control circuit board inside the controller 17 respectively. When working, the temperature sensor 12 will measure the ambient temperature inside the box 2, and compare the detected temperature signal with the target temperature set inside the controller 17. According to the comparison result, the heating ring is controlled to open to heat the inside of the box 2, or the fan 21 is turned on to draw external air through the air inlet slot 11, driving the air flow inside the box 2 to cool down, so as to maintain the ambient temperature inside the box 2 close to the target temperature for constant temperature. When the gene sample test tube is placed into the box 2, the locking rod 13 can be screwed out from the threaded hole 15 first, and the top cover 9 can be unlocked at the top of the box body 2. Then, the four groups of compression springs 5 ​​arranged on both sides of the bottom of the movable seat 6 will rebound, and the movable seat 6 can be lifted up to the opening position at the top of the box body 2. After flipping and opening the top cover 9, the test tube can be quickly inserted into the corresponding test tube slot 18 and placed. When the placement is completed and the top cover 9 is closed, the top cover 9 will use the push rod 10 to press the movable seat 6 back to the initial position inside the box body 2. When taking the test tube, after opening the top cover 9, the test tube can be automatically extended from the top of the box body 2 by the movable seat 6.

[0028] Embodiment 2: An outer cover 24 is provided on the left side of the outer cover 2, and knobs 25 are provided at the four corners at both ends of the outer cover 24, screw holes 3 are provided inside the four corners at both ends of the box 2, the knobs 25 pass through the inner part of the outer cover 24, the outer part of the knob 25 is provided with external threads, and reserved grooves are provided at both ends of the left side of the top of the outer cover 24;

[0029] Specifically, Figure 1 and Figure 4 As shown, the outer cover 24 can be inserted from the left side of the box body 2 to the outside of the box body 2, and then the knobs 25 are used to penetrate the inside of the outer cover 24 at the four corners at both ends of the outer cover 24 and screwed into the inside of the screw holes 3 respectively, and then the outer cover 24 can be fixedly installed on the outside of the box body 2. The outer cover 24 can be used to shield and protect the outside of the mechanism from collision.

[0030] Embodiment 3: A controller 17 is fixedly mounted on the right side of the box body 2, and a front cover 16 is arranged on the right side of the controller 17, connecting feet 27 are fixedly mounted on both ends of the top of the front cover 16, connecting frames 26 are fixedly mounted on both ends of the top of the controller 17, the left side of the connecting foot 27 is hingedly connected to the inside of the connecting frame 26, and a convex handle is fixedly mounted on the bottom end of the front cover 16;

[0031] Specifically, Figure 1 and, Figure 4 Figure 5As shown, when the controller 17 is in use, a front cover 16 is provided on the right side of the controller 17. The front cover 16 can assist in covering the outside of the controller 17 to avoid abnormal operation of the mechanism caused by accidental touching of the control operation buttons on the right side of the controller 17. The front cover 16 is made of transparent acrylic material and will not affect the numerical display of the controller 17.

[0032] Working principle: When the utility model is in use, the outer cover 24 provided can be inserted into the outside of the box body 2 from the left side of the box body 2, and then the knobs 25 are used at the four corners at both ends of the outer cover 24 to penetrate the inside of the outer cover 24 and screw into the inside of the screw holes 3 accordingly, so that the outer cover 24 can be fixedly installed on the outside of the box body 2. The outer cover 24 can be used to shield and protect the outside of the mechanism from collision. When the gene sample test tube is placed into the inside of the box body 2, the locking rod 13 can be screwed out from the inside of the threaded hole 15 upwards, and the top cover 9 can be unlocked at the top of the box body 2. After that, the four groups of compression springs 5 ​​provided on both sides of the bottom of the movable seat 6 will rebound, and the movable seat 6 can be lifted upward to At the opening position on the top of the box body 2, after flipping open the top cover 9, the test tube can be quickly inserted and placed in the corresponding test tube slot 18. When the placement is completed and the top cover 9 is closed, the top cover 9 will use the push rod 10 to press the movable seat 6 back to the initial position inside the box body 2. When taking the test tube, after opening the top cover 9, the test tube can be automatically extended out of the top of the box body 2 driven by the movable seat 6. When the constant temperature mechanism is in use, the constant temperature data inside the mechanism is set on the controller 17. When the controller 17 is set, a front cover 16 is set on the right side of the controller 17. The front cover 16 can be used to assist in covering the outside of the controller 17 to avoid abnormal operation of the mechanism caused by accidental touching of the control operation buttons on the right side of the controller 17.

[0033] It is obvious to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the present invention can be implemented in other specific forms without departing from the spirit or essential features of the present invention. Therefore, the embodiments should be regarded as exemplary and non-restrictive from any point of view, and the scope of the present invention is defined by the appended claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims be included in the present invention. Any reference numeral in a claim should not be regarded as limiting the claim to which it relates.

Claims

1. A constant temperature mechanism for gene extraction and amplification, comprising a box (2), characterized in that: A movable seat (6) is arranged inside the box body (2), a top cover (9) is arranged at the top of the box body (2), a mounting plate (4) is fixed at the bottom of the box body (2), and compression springs (5) are respectively fixed at both ends of the top of the mounting plate (4), top rods (10) are respectively vertically fixed at both ends of the top of the movable seat (6), fixing frames (7) are respectively installed and fixed at both ends of the top of the left side of the box body (2), fixing feet (8) are respectively installed and fixed at both ends of the left side of the top cover (9), a handle (14) is fixed on the right side of the top cover (9), a locking rod (13) is arranged on the right side of the top of the top cover (9), and a threaded hole (15) is opened at the middle position of the right side of the top of the box body (2).

2. A constant temperature mechanism for gene extraction and amplification according to claim 1, characterized in that: The top end of the compression spring (5) is fixedly connected to the bottom end of the movable seat (6), the push rod (10) is symmetrically arranged with respect to the vertical center line of the movable seat (6), and a through groove is provided inside the mounting plate (4).

3. A constant temperature mechanism for gene extraction and amplification according to claim 1, characterized in that: The bottom end of the fixed foot (8) is hingedly connected to the inside of the fixed frame (7), the locking rod (13) passes through the inside of the top cover (9), and the outside of the locking rod (13) is provided with an external thread.

4. A constant temperature mechanism for gene extraction and amplification according to claim 1, characterized in that: Guide blocks (23) are respectively fixedly mounted on both ends of the movable seat (6), and guide grooves (22) are respectively provided on both ends of the inside of the box body (2).

5. A constant temperature mechanism for gene extraction and amplification according to claim 1, characterized in that: An outer cover (24) is arranged on the left side of the exterior of the box body (2), and knobs (25) are respectively arranged at the four corners at both ends of the outer cover (24), and screw holes (3) are respectively opened inside the four corners at both ends of the box body (2).

6. A constant temperature mechanism for gene extraction and amplification according to claim 5, characterized in that: The knob (25) passes through the interior of the outer cover (24), an external thread is provided on the exterior of the knob (25), and reserved grooves are respectively provided at two ends of the left side of the top of the outer cover (24).

7. A constant temperature mechanism for gene extraction and amplification according to claim 1, characterized in that: A controller (17) is fixedly mounted on the right side of the box body (2), and a front cover (16) is arranged on the right side of the controller (17), connecting feet (27) are respectively fixed at both ends of the top of the front cover (16), and connecting frames (26) are respectively fixedly mounted at both ends of the top of the controller (17), the left side of the connecting foot (27) is hingedly connected to the inside of the connecting frame (26), and a protruding handle is fixed at the bottom end of the front cover (16).

8. A constant temperature mechanism for gene extraction and amplification according to claim 1, characterized in that: The four corners at the bottom of the box (2) are respectively fixed with feet (1), the top of the front end of the box (2) is provided with an air inlet slot (11), a temperature sensor (12) is fixedly installed on the right side of the top of the inside of the box (2), a through hole (19) is provided through the inside of the movable seat (6), a heating ring assembly (20) is fixedly installed at the bottom of the inside of the box (2), a fan (21) is fixedly installed at the bottom of the right side of the box (2), and a plurality of groups of test tube slots (18) are evenly spaced inside the top of the movable seat (6).

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