Heating structure based on PCR (Polymerase Chain Reaction) amplification instrument

Abstract

The utility model relates to the technical field of PCR (Polymerase Chain Reaction) amplification instruments, in particular to a heating structure based on a PCR amplification instrument, which comprises a linear driving component, a bearing component, a kit and a top heating component, the bearing component comprises a box seat, and one side of the box seat is provided with an open box cavity; the kit is arranged in the kit cavity and comprises a kit body and a front panel, and the front panel is fixed on one side of the kit body; when the linear driving assembly drives the bearing assembly to move to the position below the top heating assembly, the top heating assembly can heat the top of the kit. The kit has the advantages that the heating device is additionally arranged on the kit cover to form local high temperature of the kit cover so as to eliminate the local condensation phenomenon, and the kit is placed in place through the structural guide design under the condition that the kit is not placed in place.

Description

Technical Field

[0001] The utility model relates to the field of PCR amplification instruments, in particular to a heating structure based on PCR amplification instruments. Background Art

[0002] PCR amplifier, also known as PCR gene amplifier, PCR nucleic acid amplifier, polymerase chain reaction nucleic acid amplifier, is an instrument that uses PCR (Polymerase chain reaction) technology to amplify specific DNA. It is widely used in medical and biological laboratories, for example, to determine whether the specimen will show a spectrum of a certain genetic disease, diagnosis of infectious diseases, gene replication, and paternity testing.

[0003] During the PCR amplification process, DNA and RNA are usually released from biological tissue samples or cells by chemical or physical methods. For example, samples are lysed by using compounds, or by heating, or by a combination of these methods. Heating during the lysis process can improve the efficiency of lysis and shorten the lysis time, but heating can also cause evaporation of the sample liquid, and the evaporated liquid will form low-temperature condensed water when it encounters cold at the upper cover of the reagent kit, which will result in insufficient liquid volume during lysis and changes in sample concentration. This will affect the lysis effect or cause abnormal test results. Utility Model Content

[0004] The purpose of the utility model is to overcome the shortcomings of the prior art and provide a heating structure based on a PCR amplification instrument. In addition to heating the sample liquid, a heating device is added to the reagent box cover to form a local high temperature on the box cover to eliminate local condensation. In the case that the reagent box is not placed in place, the reagent box can be placed in place through a structural guiding design.

[0005] The purpose of the utility model is achieved through the following technical solutions:

[0006] The heating structure based on the PCR amplification instrument includes a linear drive component, a bearing component fixedly mounted on the linear drive component, and a reagent kit placed in the bearing component, wherein the bearing component includes a box seat, and an open box cavity is provided on the side of the box seat facing the linear drive component; the reagent kit is placed in the box cavity, and the reagent kit includes a box body and a front panel, and the front panel is fixed on the side of the box body facing the linear drive component; and further includes a top heating component, wherein the top heating component is located between the linear drive component and the bearing component, and when the linear drive component drives the bearing component to move to the bottom of the top heating component, the top heating component can heat the top of the reagent kit.

[0007] Furthermore, the linear drive assembly includes a servo motor, a screw, a drive plate, a sliding rod, a slide seat and a connecting rod. The servo motor is installed in the body of the PCR amplifier. The screw is coaxially fixedly connected to the output end of the servo motor. The two slide seats are fixedly installed in the body of the PCR amplifier. A sliding rod is installed in each slide seat. The two sliding rods are parallel to each other, and one end of the two sliding rods is fixedly installed with a drive plate. The screw is connected to the center hole of the drive plate by a threaded connection. The other ends of the two sliding rods are fixedly connected with a connecting rod, and the bearing assembly is fixedly installed on the connecting rod.

[0008] Furthermore, the top heating assembly is fixedly mounted on a slide seat.

[0009] Furthermore, the top heating assembly includes a guide rail, a slider, a heating part and a tension spring, the guide rail is vertically fixed on the slide seat, the slider is slidably mounted on the guide rail, the heating part is fixedly mounted on the slider, the upper end of the tension spring is fixed on the side wall of the slider, and the lower end is fixed on the slide seat.

[0010] Furthermore, the heating part includes a top cover, a bottom cover and a heating element, the top cover is fixedly connected to the bottom cover, the heating element is arranged between the top cover and the bottom cover, the bottom cover is a heat-conducting element, and the bottom surface of the bottom cover can heat the top of the reagent box.

[0011] Furthermore, a guiding slope is provided on a side of the bottom cover away from the linear drive assembly.

[0012] Furthermore, the heating element is a ceramic heating plate.

[0013] Furthermore, a positioning column is fixed on the side of the slide seat facing the bearing assembly; a positioning hole is opened on the side of the front panel facing the slide seat, and the positioning column corresponds to the positioning hole.

[0014] Furthermore, a slot is provided on the top of the box seat for conveniently taking out and placing the reagent box.

[0015] Furthermore, a baffle is provided on the side of the box seat facing the linear drive assembly, and the baffle is staggered with the positioning hole.

[0016] The utility model has the following advantages:

[0017] 1. The top of the reagent box is locally heated by the top heating component to form a local high temperature on the lid to eliminate local condensation. The sample concentration changes less, thereby ensuring the lysis effect and improving the accuracy of the test results.

[0018] 2. All-round heating speeds up the heating process, reduces sample lysis time, ensures consistent sample heating temperature, and ensures the effectiveness of sample lysis. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 The three-dimensional structure of the utility model is shown in FIG. Figure 1 ;

[0020] Figure 2 The three-dimensional structure of the utility model is shown in FIG. Figure 2 ;

[0021] Figure 3 It is a three-dimensional structural schematic diagram of the load-bearing assembly of the utility model;

[0022] Figure 4 It is a schematic diagram of the three-dimensional structure of the test kit of the utility model;

[0023] Figure 5 It is a schematic diagram of the three-dimensional structure of the supporting component and the reagent box of the utility model;

[0024] Figure 6 It is a schematic diagram of the structure in which the top heating component of the utility model is installed on the slide seat;

[0025] In the figure: 1-linear drive assembly, 1a-servo motor, 1b-screw, 1c-drive plate, 1d-slide, 1e-slide seat, 1e1-positioning column, 1f-connecting rod, 2-bearing assembly, 2a-box seat, 2a1-box cavity, 2a2-groove, 2b-baffle, 3-reagent box, 3a-box body, 3b-front panel, 3b1-positioning hole, 4-top heating assembly, 4a-guide rail, 4b-slider, 4c-heating part, 4c1-top cover, 4c2-bottom cover, 4c21-guide ramp, 4c3-heating element, 4d-tension spring. DETAILED DESCRIPTION

[0026] The present invention is further described below in conjunction with the accompanying drawings, but the protection scope of the present invention is not limited to the following description.

[0027] like Figures 1 to 6As shown, a heating structure based on a PCR amplification instrument includes a linear drive component 1, a carrier component 2 fixedly mounted on the linear drive component 1, and a reagent kit 3 placed in the carrier component 2, wherein the carrier component 2 includes a box seat 2a, and an open box cavity 2a1 is provided on the side of the box seat 2a facing the linear drive component 1, and the reagent kit 3 is placed in the box cavity 2a1, and the reagent kit 3 includes a box body 3a and a front panel 3b, and the front panel 3b is fixed on the side of the box body 3a facing the linear drive component 1; the utility model also includes a top heating component 4, and the top heating component 4 is located between the linear drive component 1 and the carrier component 2, and when the linear drive component 1 drives the carrier component 2 to move to the bottom of the top heating component 4, the top heating component 4 can heat the top of the reagent kit 3. When in use, the reagent box with the sample is loaded into the box cavity 2a1, and the linear drive component 1 drives the supporting component 2 and the reagent box 3 to move as a whole toward the top heating component 4. When the reagent box 3 moves to below the top heating component 4, the top heating component 4 is pressed on the top of the reagent box 3, and the top heating component 4 heats the top of the reagent box 3 (the reagent box 3 itself has a lower heating component, which is the prior art and will not be described here), and heats the top of the reagent box 3 at the same time as the original heating component of the reagent box 3, so that the sample in the reagent box 3 is heated evenly, the sample lysis efficiency is improved, and no condensation is generated on the inner top surface of the box body 3a, ensuring that the sample concentration changes little, thereby improving the accuracy of sample detection.

[0028] Furthermore, the linear drive assembly 1 includes a servo motor 1a, a screw 1b, a drive plate 1c, a slide rod 1d, a slide seat 1e and a connecting rod 1f. The servo motor 1a is installed in the body of the PCR amplifier. The screw 1b is coaxially fixedly connected to the output end of the servo motor 1a. The two slide seats 1e are fixedly installed in the body of the PCR amplifier. A slide rod 1d is installed in each slide seat 1e. The two slide rods 1d are parallel to each other, and one end of the two slide rods 1d is fixedly installed with a drive plate 1c. The screw 1b is connected to the center hole of the drive plate 1c by a threaded connection. The other ends of the two slide rods 1d are fixedly connected with a connecting rod 1f, and the bearing assembly 2 is fixedly installed on the connecting rod 1f. The servo motor 1a drives the screw 1b to rotate. Since the slide bar 1d cooperates with the slide seat 1e, the rotation of the drive plate 1c is restricted, so that the drive plate 1c moves on the screw 1b, so that the drive plate 1c can drive the slide bar 1d, the connecting rod 1f and the bearing assembly 2 to make linear reciprocating movements along the axial direction of the screw 1b.

[0029] It is understandable that this embodiment only introduces one driving mode, and other driving modes may be used as long as the bearing assembly 2 can move linearly, such as an electric telescopic cylinder, a hydraulic telescopic rod, etc.

[0030] In this embodiment, the top heating assembly 4 is fixedly mounted on the slide seat 1e.

[0031] It is understandable that, since the slide 1e is fixedly installed in the body of the PCR amplifier, the top heating component 4 can also be fixedly installed at other positions in the body of the PCR amplifier.

[0032] Furthermore, the top heating assembly 4 includes a guide rail 4a, a slider 4b, a heating part 4c and a tension spring 4d, wherein the guide rail 4a is vertically fixed on the slide 1e, the slider 4b is slidably mounted on the guide rail 4a, the heating part 4c is fixedly mounted on the slider 4b, the upper end of the tension spring 4d is fixed on the side wall of the slider 4b, and the lower end is fixed on the slide 1e. When the reagent box 3 is not moved to the bottom of the top heating assembly 4, the lower surface of the heating part 4c is lower than the upper surface of the reagent box 3. When the reagent box 3 is moved to the entrance side of the top heating assembly 4, the top surface of the reagent box 3 presses the heating part 4c and lifts the heating part 4c, so that the reagent box 3 can move to the right below the heating part 4c. Under the tension of the tension spring 4d, the slider 4b always maintains a downward movement trend, so that the heating part 4c presses the upper surface of the reagent box 3 to ensure the reliability of the contact, so that the top surface of the reagent box 3 can be heated by the heating part 4c. The heating portion 4c exerts a downward force on the reagent kit 3. If the reagent kit 3 is not placed in place before entering under the heating portion 4c, the heating portion 4c can also press the reagent kit 3 downward, thereby further placing the reagent kit 3 in place.

[0033] Further, the heating portion 4c includes a top cover 4c1, a bottom cover 4c2 and a heating element 4c3, wherein the top cover 4c1 is fixedly connected to the bottom cover 4c2, the heating element 4c3 is disposed between the top cover 4c1 and the bottom cover 4c2, the bottom cover 4c2 is a heat-conducting element, and the bottom surface of the bottom cover 4c2 can heat the top of the reagent box 3. Preferably, the top cover 4c1 is a heat-insulating element.

[0034] Furthermore, a guide slope 4c21 is provided on the side of the bottom cover 4c2 away from the linear drive component 1. When the reagent box 3 moves to the entrance of the bottom cover 4c2, the guide slope 4c21 can play a buffering role to prevent the reagent box 3 from directly contacting the side wall of the bottom cover 4c2. As the reagent box 3 moves toward the side of the linear drive component 1, the reagent box 3 abuts against the guide slope 4c21 and slowly lifts the heating part 4c.

[0035] In this embodiment, the heating element 4c3 is a ceramic heating plate.

[0036] Furthermore, a positioning column 1e1 is fixed on the side of the slide 1e facing the bearing assembly 2; a positioning hole 3b1 is opened on the side of the front panel 3b facing the slide 1e, and the positioning column 1e1 corresponds to the positioning hole 3b1. When the reagent box 3 is placed in place, as the reagent box 3 moves toward the side of the linear drive assembly 1, the positioning column 1e1 can be inserted into the positioning hole 3b1 respectively, further confirming that the reagent box 3 is placed in place.

[0037] Furthermore, a groove 2a2 is provided on the top of the cartridge holder 2a for convenient placement of the reagent kit 3. After the test is completed, the two sides of the reagent kit 3 are held by hand, and the groove 2a2 provides space for fingers to move.

[0038] Furthermore, a baffle 2b is provided on the side of the box seat 2a facing the linear drive component 1. The baffle 2b is staggered with the positioning hole 3b1. The baffle 2b can prevent the reagent box 3 from slipping out of the box cavity 2a1, and will not block the positioning column 1e1 from being inserted into the positioning hole 3b1.

[0039] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A heating structure based on a PCR amplification instrument, comprising a linear drive component (1), a carrier component (2) fixedly mounted on the linear drive component (1), and a reagent kit (3) placed in the carrier component (2), characterized in that: The bearing assembly (2) comprises a box seat (2a), and an open box cavity (2a1) is provided on a side of the box seat (2a) facing the linear drive assembly (1); The reagent box (3) is placed in the box cavity (2a1), and the reagent box (3) comprises a box body (3a) and a front panel (3b), wherein the front panel (3b) is fixed to a side of the box body (3a) facing the linear drive assembly (1); The heating structure based on the PCR amplification instrument further comprises a top heating component (4), wherein the top heating component (4) is located between the linear drive component (1) and the carrier component (2), and when the linear drive component (1) drives the carrier component (2) to move below the top heating component (4), the top heating component (4) can heat the top of the reagent box (3).

2. The heating structure based on the PCR amplification instrument according to claim 1, characterized in that: The linear drive assembly (1) comprises a servo motor (1a), a screw (1b), a drive plate (1c), a slide rod (1d), a slide seat (1e) and a connecting rod (1f); the servo motor (1a) is installed in a PCR amplification instrument body; the screw rod (1b) is coaxially fixedly connected to the output end of the servo motor (1a); two slide seats (1e) are fixedly installed in the PCR amplification instrument body; a slide rod (1d) is mounted in each slide seat (1e); the two slide rods (1d) are parallel to each other, and one end of the two slide rods (1d) is fixedly mounted with a drive plate (1c); the screw rod (1b) is connected to the center hole of the drive plate (1c) by threaded fitting; the other ends of the two slide rods (1d) are fixedly connected with a connecting rod (1f); and the bearing assembly (2) is fixedly mounted on the connecting rod (1f).

3. The heating structure based on PCR amplification instrument according to claim 2, characterized in that: The top heating component (4) is fixedly mounted on the slide seat (1e).

4. The heating structure based on PCR amplification instrument according to claim 3, characterized in that: The top heating assembly (4) comprises a guide rail (4a), a slider (4b), a heating portion (4c) and a tension spring (4d); the guide rail (4a) is vertically fixed on the slide seat (1e); the slider (4b) is slidably mounted on the guide rail (4a); the heating portion (4c) is fixedly mounted on the slider (4b); the upper end of the tension spring (4d) is fixed on the side wall of the slider (4b) and the lower end is fixed on the slide seat (1e).

5. The heating structure based on PCR amplification instrument according to claim 4, characterized in that: The heating portion (4c) comprises a top cover (4c1), a bottom cover (4c2) and a heating element (4c3); the top cover (4c1) is fixedly connected to the bottom cover (4c2); the heating element (4c3) is disposed between the top cover (4c1) and the bottom cover (4c2); the bottom cover (4c2) is a heat-conducting element, and the bottom surface of the bottom cover (4c2) can heat the top of the reagent box (3).

6. The heating structure based on PCR amplification instrument according to claim 5, characterized in that: A guiding inclined surface (4c21) is provided on a side of the bottom cover (4c2) away from the linear drive assembly (1).

7. The heating structure based on PCR amplification instrument according to claim 5, characterized in that: The heating element (4c3) is a ceramic heating plate.

8. The heating structure based on PCR amplification instrument according to claim 2, characterized in that: A positioning column (1e1) is fixed on the side of the slide seat (1e) facing the bearing assembly (2); a positioning hole (3b1) is opened on the side of the front panel (3b) facing the slide seat (1e), and the positioning column (1e1) corresponds to the positioning hole (3b1).

9. The heating structure based on PCR amplification instrument according to claim 1, characterized in that: The top of the box seat (2a) is also provided with a slot (2a2) for facilitating the taking out and placing of the reagent box (3).

10. The heating structure based on PCR amplification instrument according to claim 8, characterized in that: A baffle (2b) is also provided on the side of the box seat (2a) facing the linear drive assembly (1), and the baffle (2b) is staggered with the positioning hole (3b1).

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