Miniature centrifuge tube blending movement-free mechanism

Abstract

The utility model belongs to the technical field of biological medicine, and relates to a miniature centrifuge tube uniform mixing movement-free mechanism which comprises a centrifuge tube bearing plate, a plurality of hole sites for allowing miniature centrifuge tubes to be inserted are formed in the centrifuge tube bearing plate in the first direction, and a space capable of containing the miniature centrifuge tubes is formed below the hole sites; one side of the cover plate is provided with a bulge corresponding to the hole site, and the other side of the cover plate is provided with a strong magnet corresponding to the hole site; the damping rotating shaft comprises a first part and a second part which can relatively rotate, the first part is fixed with the centrifugal tube bearing plate, and the second part is fixed with the cover plate; and the cover plate can reach at least three positions relative to the centrifugal tube bearing plate when rotating around the damping rotating shaft. Due to the fact that the damping rotating shaft is adopted, the cover plate can rotate to three positions, the cover covering operation, the magnetic bead adsorption operation and the mixing device butt joint operation of the miniature centrifugal tubes borne in the hole positions can be achieved respectively, the miniature centrifugal tubes in the hole positions do not need to be taken out in the whole process, and operation is more convenient.

Description

Technical Field

[0001] The present application relates to the field of biomedical technology, and in particular to a micro centrifuge tube mixing and non-moving mechanism. Background Art

[0002] The magnetic bead method for extracting exosomes is to incubate the sample with pre-treated magnetic beads. The exosomes are specifically adsorbed to the magnetic beads from the sample, while other impurities are not adsorbed, thus achieving the purpose of separation. In order to separate the magnetic beads, the existing practice is to place the EP tube (microcentrifuge tube) containing the magnetic beads on a magnetic rack. Due to the effect of the magnetic force, the magnetic beads can be adsorbed on the side wall or bottom. At this time, the liquid can be discarded with a pipette to achieve the purpose of separation.

[0003] The existing magnetic rack mainly consists of test tube racks, magnetic plates and other main components. The magnetic plate and the test tube rack placement device are mostly at a vertical angle, which will cause a certain gap between the test tube and the magnetic plate when the test tube is placed, causing the magnetic force of the magnetic plate to weaken, resulting in weak adsorption of magnetic beads and prolonged adsorption time. In addition, when the magnetic beads need to be incubated, the connection between the existing magnetic rack and the mixing instrument is relatively disconnected, and the tube needs to be taken out of the magnetic rack and transferred to the mixing instrument, which is time-consuming and labor-intensive and brings inconvenience to scientific researchers. Utility Model Content

[0004] In view of this, the embodiment of the present application provides a micro centrifuge tube mixing mechanism without moving in order to solve the problem of inconvenience in using the magnetic stand in the background technology.

[0005] The present application embodiment provides a micro centrifuge tube mixing and non-moving mechanism, comprising:

[0006] A centrifuge tube carrier plate, used for carrying the micro centrifuge tubes, wherein the centrifuge tube carrier plate has a plurality of holes arranged along a first direction for inserting the micro centrifuge tubes, and a space capable of accommodating the micro centrifuge tubes is provided below the holes;

[0007] A cover plate, wherein one side of the cover plate has a protrusion arranged corresponding to the hole position, and the other side of the cover plate has a strong magnet arranged corresponding to the hole position;

[0008] The damping shaft comprises a first part and a second part capable of relative rotation, wherein the first part is fixed to the centrifuge tube bearing plate, and the second part is fixed to the cover plate;

[0009] When the cover plate rotates around the damping shaft, it can reach at least a first position, a second position and a third position relative to the centrifuge tube supporting plate.

[0010] When the cover plate is located at the first position, the protrusion on the cover plate is close to the top of the hole;

[0011] When the cover plate is located at the second position, the cover plate and the centrifuge tube supporting plate form an angle of 90° to 180° relative to the damping shaft;

[0012] When the cover plate is located at the third position, the strong magnet on the cover plate is close to one side of the space.

[0013] In an optional embodiment, when the cover is located at the third position, the strong magnet on the cover is aligned with the middle position of the space.

[0014] In an optional embodiment, the protrusion is hemispherical in shape.

[0015] In an optional embodiment, a side of the cover plate where the strong magnet is disposed has a groove, and the strong magnet is embedded in the groove and fixed to the inner wall of the groove.

[0016] In an optional embodiment, the strong magnet is cylindrical.

[0017] In an optional embodiment, the circumference of the strong magnet is provided with an external thread, the inner wall of the groove is provided with an internal thread, and the strong magnet is threadedly connected to the groove.

[0018] In an optional embodiment, the end surface of the strong magnet has a groove for a screwdriver to dock with.

[0019] In an optional implementation, the end surface of the strong magnet is in an arc shape.

[0020] In an optional embodiment, the centrifuge tube supporting plate and the cover plate are both rectangular in shape.

[0021] In an optional embodiment, the groove is a waist-shaped groove or a rectangular groove.

[0022] The micro-centrifuge tube mixing mechanism provided in the embodiment of the present application adopts a damping rotating shaft, and the cover plate can be rotated to three positions, which can respectively realize the cover operation, magnetic bead adsorption operation and docking mixer operation of the micro-centrifuge tube carried in the hole. The whole process does not require the micro-centrifuge tube in the hole to be taken out, and the operation is more convenient.

[0023] Additional aspects and advantages of the present application will be given in part in the description below, and in part will become apparent from the description below, or will be learned through the practice of the present application. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] The drawings described herein are used to provide a further understanding of the present application and constitute a part of the present application. The illustrative embodiments of the present application and their descriptions are used to explain the present application and do not constitute an improper limitation on the present application. In the drawings:

[0025] Figure 1 A schematic diagram of the upper structure of the microcentrifuge tube mixing and non-moving mechanism provided in an embodiment of the present application;

[0026] Figure 2 A schematic diagram of the structure below the microcentrifuge tube mixing and non-moving mechanism provided in an embodiment of the present application;

[0027] Figure 3 A schematic diagram of a cover plate provided in an embodiment of the present application reaching a first position;

[0028] Figure 4 A schematic diagram of a cover plate provided in an embodiment of the present application reaching a second position;

[0029] Figure 5 A schematic diagram of a cover plate provided in an embodiment of the present application reaching a third position;

[0030] Figure 6 This is a schematic diagram of the strong magnet structure provided in an embodiment of the present application.

[0031] The reference numerals in the figure are:

[0032] 1. Centrifuge tube carrier plate;

[0033] 2. Cover plate;

[0034] 3. Damping shaft;

[0035] 21. Bump;

[0036] 22. Strong magnet. DETAILED DESCRIPTION

[0037] The exemplary embodiments disclosed in the present application will be described in more detail below with reference to the accompanying drawings. Although the exemplary embodiments of the present application are shown in the accompanying drawings, it should be understood that the present application can be implemented in various forms and should not be limited by the specific embodiments described herein. On the contrary, these embodiments are provided to enable a more thorough understanding of the present application and to fully convey the scope disclosed in the present application to those skilled in the art.

[0038] In the following description, a large number of specific details are given to provide a more thorough understanding of the present application. However, it is obvious to those skilled in the art that the present application can be implemented without one or more of these details. In other examples, in order to avoid confusion with the present application, some technical features known in the art are not described; that is, all features of the actual embodiments are not described here, and well-known functions and structures are not described in detail.

[0039] In the drawings, the sizes of layers, regions, elements and their relative sizes may be exaggerated for clarity. Like reference numerals denote like elements throughout.

[0040] Spatial relationship terms such as "under", "below", "below", "under", "above", "above", etc., may be used here for convenience of description to describe the relationship between an element or feature shown in the figure and other elements or features. It should be understood that in addition to the orientation shown in the figure, the spatial relationship terms are intended to include different orientations of the device in use and operation. For example, if the device in the accompanying drawings is turned over, then the elements or features described as "under other elements" or "under it" or "under it" will be oriented as "on" other elements or features. Therefore, the exemplary terms "under" and "under" may include both upper and lower orientations. The device can be oriented otherwise (rotated 90 degrees or other orientations) and the spatial descriptors used herein are interpreted accordingly.

[0041] The purpose of the terms used herein is only to describe specific embodiments and is not intended to be limiting of the present application. When used herein, the singular forms "one", "an" and "said / the" are also intended to include plural forms, unless the context clearly indicates otherwise. It should also be understood that the terms "consisting of" and / or "comprising", when used in this specification, determine the presence of the features, integers, steps, operations, elements and / or parts, but do not exclude the presence or addition of one or more other features, integers, steps, operations, elements, parts and / or groups. When used herein, the term "and / or" includes any and all combinations of the relevant listed items.

[0042] In order to thoroughly understand the present application, detailed steps and detailed structures will be presented in the following description to illustrate the technical solution of the present application. The preferred embodiments of the present application are described in detail below, but in addition to these detailed descriptions, the present application may also have other implementation methods.

[0043] This embodiment provides a microcentrifuge tube mixing mechanism without moving. Figure 1-Figure 2 As shown, including:

[0044] A centrifuge tube carrier plate 1, used for carrying the micro centrifuge tubes, wherein the centrifuge tube carrier plate 1 has a plurality of holes arranged along a first direction for inserting the micro centrifuge tubes, and a space capable of accommodating the micro centrifuge tubes is provided below the holes;

[0045] A cover plate 2, wherein one side of the cover plate 2 has a protrusion 21 arranged corresponding to the hole position, and the other side of the cover plate 2 has a strong magnet 22 arranged corresponding to the hole position;

[0046] The damping shaft 3 comprises a first part and a second part capable of relative rotation, wherein the first part is fixed to the centrifuge tube supporting plate 1, and the second part is fixed to the cover plate 2;

[0047] When the cover plate 2 rotates around the damping shaft 3, it can at least reach a first position, a second position and a third position relative to the centrifuge tube supporting plate 1.

[0048] like Figure 3 As shown, when the cover plate 2 is located at the first position, the protrusion 21 on the cover plate 2 is close to the top of the hole;

[0049] like Figure 4 As shown, when the cover plate 2 is located at the second position, the cover plate 2 and the centrifuge tube supporting plate 1 form an angle of 90° to 180° relative to the damping shaft 3;

[0050] like Figure 5 As shown, when the cover plate 2 is located at the third position, the strong magnet 22 on the cover plate 2 is close to one side of the space.

[0051] The micro-centrifuge tube mixing and moving-free mechanism of the present embodiment adopts the damping shaft 3, and the cover plate 2 can be rotated to three positions, which can respectively realize the cover operation of the micro-centrifuge tube carried in the hole, the magnetic bead adsorption operation and the docking mixer operation. The micro-centrifuge tube in the hole does not need to be taken out during the whole process, which is more convenient to operate. Among them, the damping shaft 3 is a commonly used shaft in the prior art, and its characteristic is that after the first part and the second part of the damping shaft 3 rotate, there is damping between the two parts, so that the positional relationship after rotation is maintained. The principle of the micro-centrifuge tube mixing and moving-free mechanism of the present embodiment for carrying out the cover operation of the micro-centrifuge tube carried in the hole is as follows Figure 3 As shown, when the cover plate 2 is rotated to the first position, the protrusion 21 can just squeeze the cover into the tube mouth of the microcentrifuge tube. This operation can use the cover plate 2 to cover all the covers at once, which is quick and easy. Figure 5 As shown in FIG. 1 , when the cover plate 2 is rotated to the third position, the strong magnet 22 on the cover plate 2 is close to the side wall of the microcentrifuge tube, so that the magnetic beads in the microcentrifuge tube can be gathered at the tube wall close to the strong magnet 22, that is, the magnetic bead separation is conveniently achieved. When the magnetic beads need to be mixed with the sample for incubation, as shown in FIG. Figure 4 As shown, it is only necessary to move the cover plate 2 to an angle of 90°~180° with the centrifuge tube carrier plate 1 (180° is taken as an example in the figure). At this time, the entire microcentrifuge tube mixing and moving-free mechanism is directly adsorbed to the mixing magnetic module of the mixer by the strong magnet 22 on one side of the cover plate 2 (the mixing magnetic module is a component on the mixer that can be magnetically adsorbed, and has an adsorption surface of a magnet. The actual angle of the adsorption surface with the horizontal plane determines the actual angle that the cover plate 2 needs to rotate), and the speed is set for mixing, and there is no need to remove the microcentrifuge tube for mixing.

[0052] Optionally, the microcentrifuge tube mixing and moving-free mechanism of this embodiment, such as Figure 5 As shown, when the cover plate 2 is in the third position, the strong magnet 22 on the cover plate 2 is aligned with the middle position of the space. Under this setting, the strong magnet 22 can adsorb the magnetic beads in the microcentrifuge tube near the middle position, and the adsorption effect is better.

[0053] Optionally, the microcentrifuge tube mixing and moving-free mechanism of this embodiment, such as Figure 3 As shown, the shape of the protrusion 21 is hemispherical. The protrusion 21 of this shape can better exert force on the cover so that the cover can be stably pressed into the tube mouth of the microcentrifuge tube.

[0054] Optionally, in the microcentrifuge tube mixing and moving-free mechanism of this embodiment, the cover plate 2 has a groove on one side where the strong magnet 22 is provided, and the strong magnet 22 is embedded in the groove and fixed to the inner wall of the groove. This structural design can facilitate the disassembly, assembly or replacement of the strong magnet 22 on the cover plate 2.

[0055] Optionally, in the microcentrifuge tube mixing and moving-free mechanism of this embodiment, the strong magnet 22 is cylindrical. The cylindrical structure is convenient in manufacturing and installation, has low procurement cost, and is suitable for practical applications.

[0056] Optionally, the microcentrifuge tube mixing and moving-free mechanism of this embodiment, such as Figure 6 As shown, the circumference of the strong magnet 22 is provided with an external thread, the inner wall of the groove is provided with an internal thread, and the strong magnet 22 is threadedly connected to the groove. This structural design can be very convenient for disassembly and assembly of the strong magnet 22. Preferably, as Figure 6 As shown, the end surface of the strong magnet 22 has a groove for a screwdriver to connect, so that the strong magnet can be quickly dismantled with a screwdriver, which is simple and convenient to operate. The pattern of the groove is preferably a highly symmetrical shape, such as a cross shape, a plum blossom shape, etc., to avoid groove wear.

[0057] Optionally, the microcentrifuge tube mixing and moving-free mechanism of this embodiment, such as Figure 6 As shown, the end surface of the strong magnet 22 is in an arc shape. Under this structure, the end surface of the strong magnet 22 can match the contour of the side wall of the microcentrifuge tube, thereby improving the adsorption force on the magnetic beads inside the microcentrifuge tube.

[0058] Optionally, the microcentrifuge tube mixing and moving-free mechanism of this embodiment, such as Figure 1 As shown, the centrifuge tube supporting plate 1 and the cover plate 2 are both rectangular in shape. The rectangular shape facilitates the arrangement of multiple holes in one direction.

[0059] Optionally, in the micro-centrifuge tube mixing and non-moving mechanism of this embodiment, the groove is a waist-shaped groove or a rectangular groove. The waist-shaped groove or the rectangular groove can provide a position space for the strong magnet 22 to be adjustable, so that when micro-centrifuge tubes of different lengths are replaced, the micro-centrifuge tube mixing and non-moving mechanism of this embodiment can be adapted accordingly.

[0060] It should be understood that the above embodiments are exemplary and are not intended to include all possible implementations included in the claims. Various modifications and changes may be made on the basis of the above embodiments without departing from the scope of the present disclosure. Similarly, the various technical features of the above embodiments may be arbitrarily combined to form other embodiments of the present application that may not be explicitly described. Therefore, the above embodiments only express several implementations of the present application and do not limit the scope of protection of the patent of this application.

Claims

1. A microcentrifuge tube mixing and non-moving mechanism, characterized in that: include: A centrifuge tube carrier plate (1) for carrying the micro centrifuge tubes, the centrifuge tube carrier plate (1) having a plurality of holes arranged along a first direction for inserting the micro centrifuge tubes, and a space capable of accommodating the micro centrifuge tubes below the holes; A cover plate (2), wherein one side of the cover plate (2) has a protrusion (21) arranged corresponding to the hole position, and the other side of the cover plate (2) has a strong magnet (22) arranged corresponding to the hole position; The damping shaft (3) comprises a first part and a second part capable of relative rotation, the first part being fixed to the centrifuge tube supporting plate (1), and the second part being fixed to the cover plate (2); When the cover plate (2) rotates around the damping shaft (3), it can reach at least a first position, a second position and a third position relative to the centrifuge tube supporting plate (1). When the cover plate (2) is located at the first position, the protrusion (21) on the cover plate (2) is close to and directly above the hole; When the cover plate (2) is located at the second position, the cover plate (2) and the centrifuge tube supporting plate (1) form an angle of 90° to 180° relative to the damping shaft (3); When the cover plate (2) is located at the third position, the strong magnet (22) on the cover plate (2) is close to one side of the space.

2. The microcentrifuge tube mixing and non-moving mechanism according to claim 1, characterized in that: When the cover plate (2) is located at the third position, the strong magnet (22) on the cover plate (2) is aligned with the middle position of the space.

3. The microcentrifuge tube mixing and non-moving mechanism according to claim 1, characterized in that: The protrusion (21) is in the shape of a hemisphere.

4. The microcentrifuge tube mixing and non-moving mechanism according to claim 1, characterized in that: The cover plate (2) has a groove on one side where the strong magnet (22) is arranged, and the strong magnet (22) is embedded in the groove and fixed to the inner wall of the groove.

5. The microcentrifuge tube mixing and non-moving mechanism according to claim 4, characterized in that: The strong magnet (22) is cylindrical.

6. The microcentrifuge tube mixing and non-moving mechanism according to claim 5, characterized in that: The circumference of the strong magnet (22) is provided with an external thread, the inner wall of the groove is provided with an internal thread, and the strong magnet (22) is threadedly connected to the groove.

7. The microcentrifuge tube mixing and non-moving mechanism according to claim 6, characterized in that: The end surface of the strong magnet (22) is provided with a groove for a screwdriver to butt against.

8. The microcentrifuge tube mixing and non-moving mechanism according to claim 7, characterized in that: The end surface of the strong magnet (22) is in an arc shape.

9. The microcentrifuge tube mixing and non-moving mechanism according to any one of claims 1 to 8, characterized in that: The centrifuge tube supporting plate (1) and the cover plate (2) are both in a rectangular shape.

10. The microcentrifuge tube mixing and non-moving mechanism according to claim 4 or 5, characterized in that: The groove is a waist-shaped groove or a rectangular groove.

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