Integrated experiment case for photo-crosslinking reaction of propionium bromide azide dye

Abstract

The utility model relates to the technical field of microbiological detection, and discloses an integrated experiment case for a photo-crosslinking reaction of an azide propyl bromide dye, which comprises a case 1 with an upward opening, and the case 1 is internally divided into five areas by partition plates, namely a refrigeration supply area positioned on the front side, a heating supply area positioned on the rear side and a heating supply area positioned on the rear side, the sample reaction area and the control panel area are adjacent to the refrigeration supply area, and an air cooling heat dissipation area and an electrical assembly area corresponding to the sample reaction area and the control panel area are arranged on the rear side of the refrigeration supply area; cover plates are respectively arranged above the refrigeration supply area, the sample reaction area and the control panel area; the air-cooling heat dissipation area is communicated with the electrical assembly area, and a cover plate is arranged above the air-cooling heat dissipation area and the electrical assembly area. According to the utility model, the ice bags are placed in the refrigeration supply area, samples are uniformly mixed through the rotator, and light activation is carried out through the LED light source, so that integrated convenient operation from dark reaction to light activation is realized; the LED light source and the condensing lens are selected to enhance the performance of the light reaction, and the adverse effect of heat on the reaction is reduced through the heat dissipation system.

Description

Technical Field

[0001] The utility model relates to the technical field of microbial detection, in particular to an integrated experimental machine box for propidium azidobromide dye photocrosslinking reaction. Background Art

[0002] Propidium Monoazide (PMA) is an important fluorescent marker widely used in environmental microbiology, food safety monitoring, virus detection and microbial activity analysis. It is a photoactive dye that cannot penetrate the membrane of living cells and can selectively label and modify DNA in damaged cell membranes and exposed DNA. When exposed to strong visible light (optimal wavelength is 464nm), it forms a stable covalent bond with DNA, causing permanent modification of DNA and inhibiting its PCR amplification. Using this characteristic of propidium monoazide, researchers can effectively distinguish and screen microbial cells with complete cell structures, which is of great significance for revealing the structure and function of microbial communities, evaluating environmental health status and developing new biological products.

[0003] However, the existing technology lacks a standardized and efficient integrated experimental chassis for the photocrosslinking reaction of propidium bromide dye, resulting in the lack of systematicity and stability in sample processing and activation methods. For example, propidium bromide dye is activated prematurely under unexpected circumstances; when halogen lamps are used as light sources, the light intensity and the large amount of heat generated are difficult to control; during the dark reaction and light activation process, the sample and propidium bromide dye are not evenly mixed without damaging the cells. These factors will reduce the accuracy of the experimental data. Therefore, it is crucial to develop an efficient integrated experimental chassis for the photocrosslinking reaction of propidium bromide dye, which aims to provide stable lighting and temperature conditions to ensure sufficient mixing of samples in the reaction, while being easy to operate and low in maintenance costs, thereby improving experimental efficiency and the accuracy of results, and promoting further development of microbiology research and related application fields. Summary of the invention

[0004] In order to overcome or alleviate one or more of the above technical problems, the purpose of the utility model is to provide an integrated experimental chassis for the photocrosslinking reaction of propidium bromide dye, which enhances the performance of the photoreaction by selecting LED light source and focusing lens, reduces the adverse effects of heat on the reaction by adding a heat dissipation system, and improves the throughput and volume of sample operation by replaceable sample devices, which is conducive to obtaining better experimental results faster.

[0005] The utility model provides the following technical solutions:

[0006] An integrated experimental case for propidium bromide dye photocrosslinking reaction, comprising a casing (1) with an opening facing upward, wherein the casing (1) is divided into five areas by a partition, namely: a refrigeration supply area located at the front, a sample reaction area and a control panel area adjacent to the refrigeration supply area, and an air cooling and heat dissipation area and an electrical component area corresponding to the sample reaction area and the control panel area at the rear; a cover plate is provided above the refrigeration supply area, the sample reaction area, and the control panel area; the air cooling and heat dissipation area and the electrical component area are connected and are also provided above; the casing (1) at the bottom of the refrigeration supply area is provided with a drainage hole (11), and a partition plate on the side close to the sample reaction area is provided with a porous plate (12); the sample reaction area is provided with a A rotator (31) is provided with a plurality of sample holes, and an inner wall facing the air-cooling heat dissipation zone is provided with an illumination mechanism (33); a cover plate of the control panel zone is provided with a display panel (21) and an operation key (22) both electrically connected to the rotator (31); the air-cooling heat dissipation zone is provided with a heat dissipation element (41) connected to the illumination mechanism (33), and a cover plate above the heat dissipation element (41) is provided with a dustproof net (42); the electrical component zone is provided with a plurality of fixed partitions (51), and the fixed partitions (51) are used to fix the electrical components; a heat dissipation hole plate (52) is provided on the rear side and two side surfaces of the housing (1), and a power supply access hole (53) is provided on the heat dissipation hole plate (52) on the rear side.

[0007] According to some embodiments, the lighting mechanism (33) is an integrated LED lamp bead, and the integrated LED lamp bead emits light with a wavelength of 460 to 465 nm.

[0008] According to some embodiments, the integrated LED lamp bead is provided with a 120° focusing lens.

[0009] According to some embodiments, reflective materials are attached to the inner walls around the sample reaction area and the inner wall of the cover plate thereon.

[0010] According to some embodiments, 1.5-2 mL centrifuge tubes are placed in the plurality of sample wells (32).

[0011] According to some embodiments, the mixing speed of the rotator (31) is 20 to 80 rpm.

[0012] According to some embodiments, the heat dissipation element (41) comprises a copper tube, a heat sink and a fan, and is connected to the lighting mechanism (33) via the copper tube.

[0013] According to some embodiments, ice bags are placed in the refrigeration supply area for refrigeration.

[0014] According to some embodiments, a handle (35) is provided outside the cover plate above the sample reaction area.

[0015] Compared with the prior art, the utility model has the following beneficial effects:

[0016] The embodiment provides an integrated experimental chassis for the photocrosslinking reaction of propidium bromide dye. An ice bag is placed in the refrigeration supply area, and then the sample is placed in the sample hole. The rotator is started to perform a dark reaction while gently inverting and mixing. After several minutes, the LED light source is started for photoactivation, thereby realizing an integrated and convenient operation from dark reaction to photoactivation. The integrated experimental chassis enhances the performance of the photoreaction by selecting an LED light source and a focusing lens, reduces the adverse effects of heat on the reaction by adding a heat dissipation system, and increases the throughput and volume of sample operation by a replaceable sample device, which is conducive to obtaining better experimental results faster. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 The external schematic diagram of the integrated experimental chassis for the photo-crosslinking reaction of propidium bromide dye provided in the embodiment of the utility model.

[0018] Figure 2 This is a schematic diagram of the interior of an integrated experimental chassis for the photocrosslinking reaction of propidium bromide dye provided in an embodiment of the utility model.

[0019] Figure 3 A schematic diagram of casing partitions provided in an embodiment of the utility model.

[0020] In the figure:

[0021] Casing 1; drainage hole 11; porous plate 12; refrigeration supply area cover 13; display panel 21; operation key 22; rotator 31; sample hole 32; lighting mechanism 33; sample reaction area cover 34; handle 35; heat dissipation element 41; dustproof net 42; fixed partition 51; heat dissipation hole plate 52; power supply access hole 53; air cooling heat dissipation area and electrical component area cover 54. DETAILED DESCRIPTION

[0022] The present invention is described in detail below in conjunction with the embodiments and drawings, but it should be understood that the embodiments and drawings are only used to exemplify the present invention and do not constitute any limitation on the protection scope of the present invention. All reasonable changes and combinations within the scope of the utility model's purpose fall within the protection scope of the present invention.

[0023] In the description of the present utility model, it should be noted that the directions or positional relationships indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", "front", "rear", etc. are based on the directions or positional relationships shown in the drawings, and are only for the convenience of describing the present utility model and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific direction, be constructed and operated in a specific direction, and therefore cannot be understood as limiting the present utility model; the terms "first", "second", and "third" are only used for descriptive purposes, and cannot be understood as indicating or implying relative importance. In addition, unless otherwise clearly specified and limited, the terms "set", "install", "connect", and "connect" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be an indirect connection through an intermediate medium, or it can be a communication between the two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present utility model can be understood according to specific circumstances.

[0024] The utility model is further described below in conjunction with the accompanying drawings.

[0025] Example 1

[0026] like Figures 1 to 3 As shown, this embodiment provides an integrated experimental chassis for the photocrosslinking reaction of propidium bromide dye, which includes a housing 1 with an opening upward, and the housing 1 is divided into five areas by a partition, namely a refrigeration supply area, a sample reaction area, a control panel area, an air-cooled heat dissipation area, and an electrical component area. A drainage hole 11 is provided at the bottom of the refrigeration supply area, and a porous plate 12 that can pass cold air is installed on the partition close to the side of the sample reaction area, and a cover plate 13 is installed on the top. A display panel 21 and a plurality of operation keys 22 are provided in the control panel area; a rotator 31 is provided in the sample reaction area, and the display panel 21 is used to display the rotation speed and the running time, and the operation key 22 is used to operate the rotator 31 according to the display of the display panel 21; the rotator 31 is used to rotate and mix the sample, and it is electrically connected to the operation key 22 and the display panel 21. The rotator 31 is provided with a number of sample holes 32, and the inner wall of the partition facing the air-cooling and heat dissipation area is provided with an illumination mechanism 33, and a sample reaction area cover plate 34 is installed on the top, and a handle 35 is provided on the upper side of the sample reaction area cover plate 34. The air-cooling and heat dissipation area is provided with a heat dissipation element 41 connected to the illumination mechanism, and the heat dissipation element 41 dissipates heat for the illumination mechanism. A dustproof net 42 is provided on the top, and a fixed partition 51 matching the electrical components is provided in the electrical component area, on which electrical components such as power supplies and circuit boards are installed, and a heat dissipation hole plate 52 is installed on the rear side and the left and right sides, and a power access hole 53 is opened on the rear heat dissipation hole plate 52, and the electrical component area and the air-cooling and heat dissipation area are connected, and a cover plate 54 for the air-cooling and heat dissipation area and the electrical component area is installed on the top.

[0027] When starting the experiment, first open the cover 13 of the refrigeration supply area and put in an ice bag, which can provide a cooler environment for the sample. Then put the sample into the sample hole 32, cover the sample reaction area cover 34, press the operation key 22 in the control panel area to start the rotator 31, and set the time and speed (the speed is 20rpm), so that it can react in the dark while gently inverting to mix. After the set time is reached, a dripping sound is emitted, and the rotator 31 stops rotating. Then reset the time of the rotator 31, and start the lighting mechanism 33 for light activation. At this time, the heat dissipation elements 41 in the air-cooled heat dissipation area all start working, and the fan can also promote air flow in the box. The perforated plate structure on both sides of the fan realizes a kind of air convection, so that the sample reacts at a lower temperature.

[0028] The lighting mechanism 33 is an integrated LED lamp bead with a wavelength of 460-465nm and is equipped with a 120° focusing lens to enhance the energy of the light reaction.

[0029] Reflective materials are attached to the plates around the inner wall of the sample reaction area (including the inner side of the sample reaction area cover 34 above it) to enhance the performance during light reaction.

[0030] The sample wells 32 of the sample reaction zone rotator 31 are used to place 2 mL (up to 28 tubes are shown in the figure) centrifuge tubes, so that multiple experiments can be carried out simultaneously.

[0031] The rotation speed of the rotator 31 in the sample reaction zone during mixing is 20-80 rpm per minute, which improves the performance of the sample when reacting with PMA.

[0032] The air-cooled heat dissipation zone is provided with a heat dissipation element 41 connected to the lighting mechanism 33, which is a copper tube, a heat sink and a fan. The two sides and the rear side thereof are provided with a heat dissipation hole plate 52 structure, which reduces the adverse effects of the heating of components on the experiment.

[0033] During operation, put an ice bag in the refrigeration supply area, then put the sample into the sample hole, start the rotator, let it undergo dark reaction while gently inverting to mix, after several minutes, start the LED light source for light activation, and realize the integrated and convenient operation from dark reaction to light activation.

[0034] The above embodiments are only preferred implementations of the present invention, and the protection scope of the present invention is not limited to the above embodiments. All technical solutions under the concept of the present invention belong to the protection scope of the present invention. It should be pointed out that for ordinary technicians in this technical field, improvements and modifications without departing from the principle of the present invention should also be regarded as the protection scope of the present invention.

Claims

1. An integrated experimental cabinet for photocrosslinking reaction of propidium bromide dye, comprising a cabinet (1) with an opening facing upward, characterized in that: The housing (1) is divided into five areas by a partition, namely: a refrigeration supply area located at the front, a sample reaction area and a control panel area adjacent to the refrigeration supply area, and a wind cooling and heat dissipation area and an electrical component area corresponding to the sample reaction area and the control panel area at the rear; a cover plate is provided above the refrigeration supply area, the sample reaction area, and the control panel area; the wind cooling and heat dissipation area and the electrical component area are connected and are also provided above a cover plate; The casing (1) at the bottom of the refrigeration supply area is provided with a drainage hole (11), and a partition plate on one side close to the sample reaction area is provided with a porous plate (12); A rotator (31) is provided in the sample reaction area, a plurality of sample holes are provided on the rotator (31), and an illumination mechanism (33) is provided on the inner wall facing the air cooling and heat dissipation area; The cover plate of the control panel area is provided with a display panel (21) and operation keys (22) both electrically connected to the gyroscope (31); The air-cooled heat dissipation zone is provided with a heat dissipation element (41) connected to the lighting mechanism (33), and the cover plate above the heat dissipation element (41) is provided with a dustproof net (42); The electrical component area is provided with a plurality of fixed partitions (51), the fixed partitions (51) are used to fix the electrical components, the rear side and two side surfaces of the housing (1) are provided with heat dissipation holes (52), and the heat dissipation holes (52) on the rear side are provided with power access holes (53).

2. The integrated experimental cabinet for photo-crosslinking reaction of propidium bromide dye according to claim 1, characterized in that: The lighting mechanism (33) is an integrated LED lamp bead, and the integrated LED lamp bead emits light with a wavelength of 460 to 465 nm.

3. The integrated experimental cabinet for photo-crosslinking reaction of propidium bromide dye according to claim 2, characterized in that: The integrated LED lamp bead is provided with a 120° focusing lens outside.

4. The integrated experimental cabinet for photo-crosslinking reaction of propidium bromide dye according to claim 1, characterized in that: The inner walls around the sample reaction area and the inner wall of the cover plate thereon are all attached with reflective materials.

5. The integrated experimental cabinet for photo-crosslinking reaction of propidium bromide dye according to claim 1, characterized in that: A plurality of the sample holes (32) are filled with 1.5-2 mL centrifuge tubes.

6. The integrated experimental cabinet for photo-crosslinking reaction of propidium bromide dye according to claim 1, characterized in that: The mixing speed of the rotator (31) is 20 to 80 rpm.

7. The integrated experimental cabinet for photo-crosslinking reaction of propidium bromide dye according to claim 1, characterized in that: The heat dissipation element (41) comprises a copper tube, a heat sink and a fan, and is connected to the lighting mechanism (33) via the copper tube.

8. The integrated experimental cabinet for propidium bromide azido dye photocrosslinking reaction according to claim 1, characterized in that: Ice bags are placed in the refrigeration supply area for refrigeration.

9. The integrated experimental cabinet for photo-crosslinking reaction of propidium bromide dye according to any one of claims 1 to 8, characterized in that: A handle (35) is provided outside the cover plate above the sample reaction area.

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