Hepatocyte separation device for hepatocyte damage model rat

Abstract

The utility model discloses a hepatocyte separation device for a hepatocyte damage model rat, and relates to the technical field of centrifugal devices. The device comprises a shell, an inlet and outlet is formed in the middle of the top face of the shell, a centrifugal control assembly is arranged in the shell, an electric push rod is fixedly installed on the surface of the centrifugal control assembly, and the telescopic end of the electric push rod is movably sleeved with a supporting frame; the surface of the supporting frame is provided with a test tube placing assembly used for storing hepatocyte sample test tubes, the surface of the telescopic end of the electric push rod is provided with a positioning assembly used for limiting the supporting frame, the centrifugal control assembly comprises a motor, and the motor is fixedly installed at the bottom of the inner wall of the shell. During use, the effect of conveniently taking and placing centrifugal hepatocyte sample test tubes is achieved, the situation that the test tubes collide with the device in the test tube moving process is avoided, and the centrifugal work is more convenient.

Description

Technical Field

[0001] The utility model relates to the technical field of centrifugal devices, in particular to a liver cell separation device for rats with liver cell damage model. Background Art

[0002] In the liver cell damage model, researchers may induce liver damage or disease simulation in rats through different methods (such as drug treatment, poison injection, etc.), and then use the liver cell separation device to obtain these damaged liver cells. These separated liver cells can be used to study the mechanism of liver damage, cell repair process, drug efficacy, etc.

[0003] A Chinese patent document with publication number CN216513778U discloses a hepatocyte separation device for rats with hepatocyte damage model, which relates to the field of cell separation technology and includes a device body, a centrifugal disk is arranged on the outer surface of the device body, a rotating bracket is arranged inside the centrifugal disk, and a holding bottle is arranged on the outer side of the rotating bracket. With respect to the above technical scheme, the following defects still exist: when separating hepatocytes, it is necessary to place the test tube containing the hepatocyte sample on the rotating bracket located in the recessed area. When placing or taking the test tube, it is easy to collide with the surface of the device, which may cause the test tube to break, so the process of placing and taking the test tube is relatively inconvenient.

[0004] Therefore, a device for separating liver cells from rats with liver cell damage model is proposed. Utility Model Content

[0005] The purpose of the utility model is to solve the problems mentioned in the above background technology, and provide a device for separating liver cells of rats with liver cell damage model.

[0006] In order to achieve the above-mentioned purpose, the utility model specifically adopts the following technical solutions:

[0007] A hepatocyte separation device for rats with hepatocyte damage model comprises a shell, an entrance and exit are opened in the middle of the top surface of the shell, a centrifugal control component is arranged inside the shell, an electric push rod is fixedly mounted on the surface of the centrifugal control component, a support frame is movably sleeved on the telescopic end of the electric push rod, a test tube placement component for storing hepatocyte sample test tubes is arranged on the surface of the support frame, and a positioning component for limiting the support frame is arranged on the telescopic end surface of the electric push rod.

[0008] Furthermore, the centrifugal control component includes a motor, and the motor is fixedly mounted on the bottom of the inner wall of the shell, the output end of the motor is fixedly connected to a rotating shaft, the end of the rotating shaft is fixedly connected to a mounting tube, and the electric push rod is fixedly mounted inside the mounting tube, the inner wall of the shell is fixedly connected to a partition, and the partition is rotatably connected to the mounting tube, and the front of the shell is provided with buttons and a display screen for controlling the motor.

[0009] Furthermore, the positioning assembly includes a baffle, and the baffle is fixedly sleeved on the telescopic end surface of the electric push rod, and the top surface of the baffle is fitted with the bottom surface of the support frame, the top surface of the baffle is fixedly connected to a limiting column, a limiting hole is penetrated through the surface of the support frame, and the inner wall of the limiting hole is movably connected to the limiting column, the telescopic end of the electric push rod is threadedly connected with a threaded sleeve, and the bottom surface of the threaded sleeve is fitted with the top surface of the support frame.

[0010] Furthermore, the test tube placement assembly includes a first fixing ring, and the first fixing ring is fixedly connected to the end of the support frame, the first fixing ring is fixedly connected to the ground with a connecting rod, the bottom end of the connecting rod is fixedly connected to a second fixing ring, the top surface of the second fixing ring is provided with a supporting groove, and the top surface of the first fixing ring is penetrated by a sliding hole.

[0011] Furthermore, an anti-skid gasket is fixedly connected to the inner wall of the sliding hole, and the anti-skid gasket is made of rubber.

[0012] Furthermore, a cover plate is hingedly connected to the top surface of the shell, and an observation glass is embedded in the middle of the cover plate.

[0013] The beneficial effects of the utility model are as follows:

[0014] A test tube containing a rat liver cell sample is placed on the surface of the test tube placement assembly, the test tube is limited by the test tube placement assembly, and the telescopic end is driven to shorten by the electric push rod, thereby driving the support frame and the test tube placement assembly to descend, so that the test tube passes through the entrance and exit into the interior of the shell, and the centrifugal control assembly is operated to drive the electric push rod, the support frame and the test tube placement assembly to rotate, thereby driving the test tube to rotate at high speed, thereby centrifuging the liver cells. After the centrifugation is completed, the electric push rod can drive the support frame and the test tube placement assembly to rise, so that the test tube leaves the interior of the shell, and the support frame and the test tube placement assembly can be disassembled by releasing the limiting effect of the positioning assembly, so as to transport batches of test tubes and samples, and the effect of facilitating the picking up and placing of the centrifuged liver cell sample test tubes is achieved during use, avoiding the collision of the test tube with the device during the movement of the test tube, and making the centrifugation work more convenient. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 It is a three-dimensional structural schematic diagram of the utility model;

[0016] Figure 2 It is a side sectional view of the structure of the utility model;

[0017] Figure 3 This is an enlarged view of the positioning component structure of the utility model;

[0018] Figure 4 This is a schematic diagram of the structure of the test tube placement component of the utility model;

[0019] Figure numerals: 1. Shell; 2. Centrifugal control assembly; 201. Motor; 202. Rotating shaft; 203. Partition; 204. Mounting tube; 205. Button; 206. Display screen; 3. Electric push rod; 4. Support frame; 5. Positioning assembly; 501. Baffle; 502. Limiting column; 503. Limiting hole; 504. Threaded sleeve; 6. Test tube placement assembly; 601. First fixing ring; 602. Second fixing ring; 603. Connecting rod; 604. Sliding hole; 605. Supporting groove; 606. Anti-slip gasket; 7. Cover plate; 8. Observation glass; 9. Entrance and exit. DETAILED DESCRIPTION

[0020] In order to make the purpose, technical scheme and advantages of the embodiments of the utility model clearer, the technical scheme in the embodiments of the utility model will be clearly and completely described below in conjunction with the drawings in the embodiments of the utility model. Obviously, the described embodiments are part of the embodiments of the utility model, not all of the embodiments. Generally, the components of the embodiments of the utility model described and shown in the drawings here can be arranged and designed in various different configurations.

[0021] Therefore, the following detailed description of the embodiments of the present invention provided in the accompanying drawings is not intended to limit the scope of the present invention to be protected, but merely represents selected embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0022] It should be noted that similar reference numerals and letters represent similar items in the following drawings, so once an item is defined in one drawing, it does not need to be further defined and explained in the subsequent drawings. In addition, the terms "first", "second", etc. are only used to distinguish the description and cannot be understood as indicating or implying relative importance.

[0023] The electrical components mentioned in this article are all connected to an external main controller and 220V mains electricity, and the main controller can be a conventional known device for controlling a computer or the like.

[0024] In the description of the embodiments of the present invention, it should be noted that the terms "inside", "outside", "upper", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, or are the orientations or positional relationships in which the utility model product is usually placed when in use. They are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention.

[0025] like Figures 1 to 4 As shown, a liver cell separation device for a rat with a liver cell damage model comprises a shell 1, an entrance and exit 9 is opened in the middle of the top surface of the shell 1, a centrifugal control component 2 is arranged inside the shell 1, an electric push rod 3 is fixedly mounted on the surface of the centrifugal control component 2, a support frame 4 is movably sleeved on the telescopic end of the electric push rod 3, a test tube placement component 6 for storing liver cell sample test tubes is arranged on the surface of the support frame 4, and a positioning component 5 for limiting the support frame 4 is arranged on the telescopic end surface of the electric push rod 3. More specifically, a test tube containing a rat liver cell sample is placed on the surface of the test tube placement component 6, the test tube is limited by the test tube placement component 6, and the telescopic end of the electric push rod 3 is driven to shorten, thereby driving the support frame 4 and the test tube placement component 6 to descend, so that the test tube passes through the entrance and exit 9 into the interior of the shell 1, and the centrifugal control component 2 is operated to drive the electric push rod 3, the support frame 4 and the test tube placement component 6 to rotate, thereby driving the test tube to rotate at high speed, thereby centrifuging the liver cells. After centrifugation is completed, the electric push rod 3 can drive the support frame 4 and the test tube placement component 6 to rise, so that the test tube leaves the interior of the shell 1, and by releasing the limiting effect of the positioning component 5, the support frame 4 and the test tube placement component 6 can be disassembled, thereby transporting batches of test tubes and samples.

[0026] The centrifugal control assembly 2 includes a motor 201, and the motor 201 is fixedly mounted on the bottom of the inner wall of the housing 1, the output end of the motor 201 is fixedly connected with a rotating shaft 202, the end of the rotating shaft 202 is fixedly connected with a mounting tube 204, and the electric push rod 3 is fixedly mounted inside the mounting tube 204, the inner wall of the housing 1 is fixedly connected with a partition 203, and the partition 203 is rotatably connected with the mounting tube 204, and the front of the housing 1 is provided with a button 205 and a display screen 206 for controlling the motor 201. It should be noted that the operation of the motor 201 is controlled by the button 205, thereby driving the rotating shaft 202 and the mounting tube 204 to rotate, thereby driving the electric push rod 3, the support frame 4 and the test tube placement assembly 6 to rotate, thereby driving the test tube to rotate centrifugally.

[0027] The positioning assembly 5 includes a baffle 501, and the baffle 501 is fixedly sleeved on the telescopic end surface of the electric push rod 3, and the top surface of the baffle 501 is in contact with the bottom surface of the support frame 4, the top surface of the baffle 501 is fixedly connected to the limiting column 502, the surface of the support frame 4 is penetrated with a limiting hole 503, and the inner wall of the limiting hole 503 is movably plugged with the limiting column 502, the telescopic end of the electric push rod 3 is threadedly connected with a threaded sleeve 504, and the bottom surface of the threaded sleeve 504 is in contact with the top surface of the support frame 4. More specifically, by rotating the threaded sleeve 504, the threaded sleeve 504 and the telescopic end of the electric push rod 3 are disconnected, and the support frame 4 is pulled upward to drive the inner wall of the limiting hole 503 to be separated from the surface of the limiting column 502, so that the limit of the support frame 4 can be released, so that all the test tubes can be taken out of the device surface.

[0028] The test tube placement assembly 6 includes a first fixing ring 601, and the first fixing ring 601 is fixedly connected to the end of the support frame 4, the ground of the first fixing ring 601 is fixedly connected to a connecting rod 603, the bottom end of the connecting rod 603 is fixedly connected to a second fixing ring 602, the top surface of the second fixing ring 602 is provided with a supporting groove 605, and the top surface of the first fixing ring 601 is penetrated with a sliding hole 604. It should be noted that the test tube containing the hepatocyte sample is inserted into the sliding hole 604, so that the bottom of the test tube is placed inside the supporting groove 605, and the test tube is limited by the cooperation of the sliding hole 604 and the supporting groove 605.

[0029] The inner wall of the sliding hole 604 is fixedly connected with an anti-skid washer 606, and the anti-skid washer 606 is made of rubber. More specifically, by providing the anti-skid washer 606 made of rubber, the friction with the surface of the test tube is increased, making the placement of the test tube more stable and preventing the test tube from colliding with the inner wall of the sliding hole 604.

[0030] The top surface of the housing 1 is hinged with a cover plate 7, and an observation glass 8 is embedded in the middle of the cover plate 7. It should be noted that before the centrifugation operation, the cover plate 7 is rotated first so that the cover plate 7 fits the top surface of the housing 1, and the inlet and outlet 9 are blocked to avoid the inside and outside situation during the centrifugation process. By setting the observation glass 8, it is convenient to observe the centrifugation of liver cells at any time.

[0031] In summary: a test tube containing a rat hepatocyte sample is placed on the surface of the test tube placement component 6, the test tube is limited by the test tube placement component 6, and the telescopic end is driven to shorten by the electric push rod 3, thereby driving the support frame 4 and the test tube placement component 6 to descend, so that the test tube passes through the entrance and exit 9 and enters the interior of the shell 1, and the centrifugal control component 2 is operated to drive the electric push rod 3, the support frame 4 and the test tube placement component 6 to rotate, thereby driving the test tube to rotate at high speed, thereby centrifuging the hepatocytes. After centrifugation is completed, the electric push rod 3 can drive the support frame 4 and the test tube placement component 6 to rise, so that the test tube leaves the interior of the shell 1, and by releasing the limiting effect of the positioning component 5, the support frame 4 and the test tube placement component 6 can be disassembled, so as to transport batches of test tubes and samples. In use, the effect of facilitating the picking up and placing of the centrifuged hepatocyte sample test tube is achieved, and the collision with the device during the movement of the test tube is avoided, making the centrifugation work more convenient.

[0032] The above shows and describes the basic principle, main features and advantages of the utility model. Those skilled in the art should understand that the utility model is not limited by the above embodiments. The above embodiments and the specification only describe the principles of the utility model. The utility model may be subject to various changes and improvements without departing from the spirit and scope of the utility model. These changes and improvements fall within the scope of the utility model to be protected. The scope of protection claimed by the utility model is defined by the attached claims and their equivalents.

Claims

1. A device for separating liver cells from rats with liver cell damage model, characterized in that: The invention comprises a shell (1), wherein an entrance (9) is provided in the middle of the top surface of the shell (1), a centrifugal control assembly (2) is arranged inside the shell (1), an electric push rod (3) is fixedly mounted on the surface of the centrifugal control assembly (2), a support frame (4) is movably sleeved on the telescopic end of the electric push rod (3), a test tube placement assembly (6) for storing a liver cell sample test tube is arranged on the surface of the support frame (4), and a positioning assembly (5) for limiting the support frame (4) is arranged on the telescopic end surface of the electric push rod (3).

2. A hepatocyte separation device for rats with hepatocyte damage model according to claim 1, characterized in that: The centrifugal control component (2) comprises a motor (201), and the motor (201) is fixedly mounted on the bottom of the inner wall of the housing (1); the output end of the motor (201) is fixedly connected to a rotating shaft (202); the end of the rotating shaft (202) is fixedly connected to a mounting tube (204); the electric push rod (3) is fixedly mounted inside the mounting tube (204); the inner wall of the housing (1) is fixedly connected to a partition (203), and the partition (203) is rotatably connected to the mounting tube (204); and the front of the housing (1) is provided with a button (205) and a display screen (206) for controlling the motor (201).

3. The device for separating liver cells of rats with liver cell damage model according to claim 1, characterized in that: The positioning assembly (5) comprises a baffle (501), and the baffle (501) is fixedly sleeved on the telescopic end surface of the electric push rod (3), and the top surface of the baffle (501) is in contact with the bottom surface of the support frame (4), the top surface of the baffle (501) is fixedly connected to a limiting column (502), a limiting hole (503) is provided through the surface of the support frame (4), and the inner wall of the limiting hole (503) is movably connected to the limiting column (502), and the telescopic end of the electric push rod (3) is threadedly connected with a threaded sleeve (504), and the bottom surface of the threaded sleeve (504) is in contact with the top surface of the support frame (4).

4. The device for separating liver cells of rats with liver cell damage model according to claim 1, characterized in that: The test tube placement assembly (6) comprises a first fixing ring (601), and the first fixing ring (601) is fixedly connected to the end of the support frame (4), the first fixing ring (601) is fixedly connected to the ground with a connecting rod (603), the bottom end of the connecting rod (603) is fixedly connected to a second fixing ring (602), the top surface of the second fixing ring (602) is provided with a supporting groove (605), and the top surface of the first fixing ring (601) is penetrated by a sliding hole (604).

5. The device for separating liver cells of rats with liver cell damage model according to claim 4, characterized in that: The inner wall of the sliding hole (604) is fixedly connected with an anti-skid gasket (606), and the anti-skid gasket (606) is made of rubber.

6. The device for separating liver cells of rats with liver cell damage model according to claim 1, characterized in that: A cover plate (7) is hingedly connected to the top surface of the housing (1), and an observation glass (8) is embedded in the middle of the cover plate (7).

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