Pore plate device with position error prevention function

Abstract

The utility model provides a pore plate device with a position error prevention function, which comprises a pore plate and a bottom plate, and is characterized in that the structures of the pore plate and the bottom plate are improved, a bulge is arranged at one corner of the bottom plate, a bead is arranged at one corner of the pore plate, and when the position of the pore plate on the bottom plate is wrongly placed, the bead and the bulge meet, so that the pore plate cannot be flatly placed; meanwhile, a bar code is arranged on one side of the pore plate, a code scanner is arranged on one side of the bottom plate, and when the pore plate is correctly placed on the bottom plate, the bar code can be recognized by the code scanner; two reagent tubes are selected in the reagent tubes of the pore plate and are respectively used for fixedly placing a high-concentration quality control sample and a low-concentration quality control sample. The pore plate device provided by the utility model is simple in structure and convenient to use, provides practical guarantee for correct placement of the pore plate on the bottom plate, and is suitable for large-scale popularization and application.

Description

Technical Field

[0001] The invention belongs to the field of biotechnology, and in particular relates to a well plate device with a function of preventing position errors. Background Art

[0002] Neonatal genetic metabolic disease screening is a system service that uses fast, simple and sensitive technology on newborns to screen for congenital and genetic diseases that endanger children's lives, harm children's growth and development, and cause intellectual disabilities. It makes an early diagnosis before the child's biochemical hormone levels change significantly and before symptoms appear. Through effective treatment, it avoids irreversible damage to the child's important organs and ensures the normal physical and intellectual development of children. It is an effective way to improve the quality of the newborn population. With the increase in the number of screened diseases and the upgrade of detection project methods, in order to adapt to the needs of new project upgrades and quality control requirements, we have examined the deficiencies in the existing method flow and found that in the actual screening process, the position of the well plate may be incorrect, resulting in errors in the corresponding test results.

[0003] In order to ensure that the quality of the screening project is controllable and the experimental results are traceable, it is necessary to provide timely warnings and corrections on whether the position of the orifice plate is correctly placed during the actual screening process, so as to avoid subsequent problems caused by incorrect placement of the orifice plate on the base plate. Utility Model Content

[0004] In order to solve the above problems, the utility model provides a well plate device with a function of preventing position errors, including a well plate and a bottom plate. By improving the structures of the well plate and the bottom plate, a protrusion is set at one corner of the bottom plate, and a convex ridge is set at one corner of the well plate. When the well plate is placed incorrectly on the bottom plate, the convex ridge meets the protrusion, making it impossible for the well plate to be laid flat; at the same time, a barcode is set on one side of the well plate, and a barcode scanner is set on one side of the bottom plate. When the well plate is placed correctly on the bottom plate, the barcode can be recognized by the barcode scanner; and in the reagent tube of the well plate, two reagent tubes are selected to respectively fix and place high-concentration quality control samples and low-concentration quality control samples. The well plate device provided by the utility model has a simple structure and is easy to use. It provides a practical guarantee for the correct placement of the well plate on the bottom plate, and is suitable for large-scale promotion and application.

[0005] The utility model provides an orifice plate device with a function of preventing position errors, comprising an orifice plate and a bottom plate, wherein a protrusion is provided at one corner of the bottom plate, and a convex ridge is provided at one corner of the orifice plate; when the orifice plate is placed incorrectly on the bottom plate, the convex ridge on the orifice plate meets the protrusion on the bottom plate, so that the orifice plate cannot be laid flat.

[0006] Furthermore, reagent tubes are provided in the orifice plate, but there are no reagent tubes at the four corners of the orifice plate. Therefore, the corners without ridges in the orifice plate are in a suspended state below and can be smoothly laid flat on the protrusions, but the corners with ridges will be lifted up because the ridges hit the protrusions and cannot be laid flat.

[0007] Furthermore, a rectangular plane plate having a size matching that of the orifice plate is provided on the bottom plate, and the orifice plate can be completely placed above the rectangular plane plate of the bottom plate.

[0008] The bottom plate is provided with a rectangular flat plate whose size matches the orifice plate, which means that the bottom plate is provided with a size that is consistent with or slightly larger than the orifice plate, so that the orifice plate can be stably placed on the bottom plate for the next step of operation. In actual operation, one or more rectangular flat plates can be provided on the bottom plate to place multiple orifice plates, such as 1, 2, 4, 6 orifice plates, etc. The utility model is described for a bottom plate with only 1 rectangular panel and only one orifice plate, and other situations can be deduced by analogy.

[0009] Since both the bottom plate and the orifice plate are rectangular, when the orifice plate is placed on the bottom plate, the long side of the orifice plate is parallel to the long side of the bottom plate (rectangular flat plate), and the short side of the orifice plate is parallel to the short side of the bottom plate (rectangular flat plate). However, both the orifice plate and the bottom plate have two long sides and two short sides. When placed correctly, the first long side of the orifice plate must be close to the first long side of the bottom plate, and the second long side of the orifice plate must be close to the second long side of the bottom plate. At this time, the first short side of the orifice plate is also close to the first short side of the bottom plate, and the second short side of the orifice plate is also close to the second short side of the bottom plate.

[0010] The term "close" means that the first long side of the orifice plate is closer to the first long side of the base plate (rectangular plane plate) than the second long side, and the second long side of the orifice plate is closer to the second long side of the base plate than the first long side, that is, the first long side of the orifice plate is in a position above the first long side of the base plate, and the second long side of the orifice plate is in a position above the second long side of the base plate.

[0011] At the same time, the well plate has a front and back side, and must be used with the front side facing up, that is, the opening of the reagent tube in the well plate facing up. In actual operation, it is impossible to place the well plate with the back side facing up due to placement errors, which will make subsequent testing impossible.

[0012] Therefore, there are only two ways to place the orifice plate on the base plate. The first is the correct way: the first long side of the orifice plate is close to the first long side of the base plate, and the second long side of the orifice plate is close to the second long side of the base plate; the second is the wrong way: the first long side of the orifice plate is close to the second long side of the base plate, and the second long side of the orifice plate is close to the first long side of the base plate.

[0013] If the well plate is placed on the bottom plate in the second incorrect placement due to an operational error, each sample tested on the well plate will not correspond to the test result, which will seriously affect the results of neonatal genetic metabolic disease screening.

[0014] The utility model improves the structures of the orifice plate and the bottom plate. When the orifice plate is placed on the bottom plate in the second incorrect placement manner, one end of the orifice plate will be lifted and cannot be laid flat, thereby giving an early warning of the incorrect placement manner and reminding the operator to correct it to the correct placement manner as soon as possible (equivalent to rotating the orifice plate horizontally 180° and then placing it again).

[0015] The shapes of the ridges and protrusions shown can be any shape, such as triangular prism, cylinder, vertical plate, triangular pyramid, cone, etc. The shapes of the ridges and protrusions can be the same or different, as long as the orifice plate cannot be laid flat on the bottom plate when the ridges and protrusions meet, and the orifice plate can be laid flat on the bottom plate smoothly when they do not meet.

[0016] Furthermore, when the orifice plate is correctly placed on the base plate, the corner with the ridge on the orifice plate and the corner with the protrusion on the base plate are respectively located at two corners corresponding to the diagonal of the orifice plate or the base plate; however, when the orifice plate is incorrectly placed on the base plate, the corner with the ridge on the orifice plate and the corner with the protrusion on the base plate are located at the same position, and the ridge is located above the protrusion, causing the orifice plate to be partially raised.

[0017] In order to make the ridge of the orifice plate meet the protrusion of the bottom plate when the orifice plate is placed incorrectly, the ridge of the orifice plate and the protrusion of the bottom plate must be at the diagonal position when the orifice plate is placed correctly. For example, when the ridge of the orifice plate is set at the left corner position of the second long side of the orifice plate, and the protrusion of the bottom plate is set at the right corner position of the first long side of the bottom plate, once the orifice plate is placed incorrectly, the orifice plate is rotated horizontally 180°, and the left corner of the second long side of the orifice plate will be transferred to the right corner position of the first long side of the bottom plate, so that the ridge of the orifice plate meets the protrusion of the bottom plate, and the orifice plate cannot be placed flat; for another example, when the ridge of the orifice plate is set at the left corner position of the first long side of the orifice plate, and the protrusion of the bottom plate is set at the right corner position of the second long side of the bottom plate, once the orifice plate is placed incorrectly, the orifice plate is rotated horizontally 180°, and the left corner of the first long side of the orifice plate will be transferred to the right corner position of the second long side of the bottom plate, so that the ridge of the orifice plate meets the protrusion of the bottom plate, and the orifice plate cannot be placed flat.

[0018] It can be understood that there are four such settings. The ridges of the orifice plate can be set at the left corner ①, right corner ② of the first long side of the orifice plate, the left corner ③, and right corner ④ of the second long side. The corresponding protrusions of the bottom plate must be set at the right corner ①, left corner ② of the second long side, the right corner ③, and left corner ④ of the first long side. It only needs to be ensured that the corners with ridges on the orifice plate and the corners with protrusions on the bottom plate are respectively located at the two corners corresponding to the diagonal lines of the orifice plate or the bottom plate.

[0019] Furthermore, the well plate includes any one or more of a 32-well plate, a 96-well plate, and a 384-well plate.

[0020] Furthermore, the well plate is a 96-well plate, and the bottom plate is provided with a rectangular flat plate with the same size as the 96-well plate.

[0021] Furthermore, the four corners on the 96-well plate are corner A1, corner A12, corner H1, and corner H12, which should be placed above the four corners A1', corner A12', corner H1', ​​and corner H12' of the bottom plate, respectively; the H1 corner of the 96-well plate is provided with a convex ridge, and the A12' corner of the bottom plate is provided with a protrusion.

[0022] Furthermore, a barcode is provided on one side of the orifice plate, and a barcode scanner is provided on one side of the bottom plate. When the orifice plate is placed correctly on the bottom plate, the barcode on the orifice plate can be recognized by the barcode scanner, but when the orifice plate is placed incorrectly on the bottom plate, the barcode scanner cannot scan the barcode.

[0023] Furthermore, the barcode is located on the side wall of one side of the orifice plate, and the barcode scanner is located on one side of the bottom plate, and can be located on the bottom plate or outside the bottom plate, but the barcode must be within the scanning range of the barcode scanner.

[0024] In some methods, a barcode is printed on the vertical side wall of the first long side of the orifice plate, and a barcode scanner is provided on the outer side of the first long side close to the bottom plate. When the orifice plate is correctly placed on the bottom plate (the first long side of the orifice plate is close to the first long side of the bottom plate, and the second long side of the orifice plate is close to the second long side of the bottom plate), the barcode is within the scanning range of the barcode scanner and can be recognized by the barcode scanner; when the orifice plate is incorrectly placed on the bottom plate (the first long side of the orifice plate is close to the second long side of the bottom plate, and the second long side of the orifice plate is close to the first long side of the bottom plate), the barcode cannot be recognized by the barcode scanner because it is on the other side. At this time, the operator will be reminded to correct the placement as soon as possible.

[0025] Furthermore, in the reagent tubes of the well plate, reagent tubes at specific positions are selected to fix the quality control samples.

[0026] In some embodiments, a reagent tube at one or more specific positions in a selected well plate is fixed with a quality control sample.

[0027] In some embodiments, a reagent tube at one or more specific positions in a selected well plate is fixedly placed with a high-concentration quality control sample or a low-concentration quality control sample.

[0028] In some embodiments, reagent tubes at two specific positions in the selected well plate are fixedly placed with high-concentration quality control samples or low-concentration quality control samples.

[0029] By fixing the quality control products at specific positions in the orifice plate, it can also help to warn of incorrect placement of the orifice plate.

[0030] There are two types of dried blood spots placed in the well plate for testing, one is the newborn blood spot to be tested (or called the sample to be tested), and the other is the quality control blood spot (low and high concentrations each occupy one well). The test value of the sample to be tested is unknown, but it is generally in a lower level range, for example, about the single digit; the quality control blood spot generally has two concentration levels, for example, the low concentration is about 10, and the high concentration level is about 30. In general, the test value of the quality control will be significantly different from the sample to be tested.

[0031] According to this feature, by inserting the blood spot of the quality control product into the specific hole position in the well plate, it is possible to judge whether the position of the well plate is placed correctly according to the test results. For example, it is stipulated that the low-concentration quality control blood spot and the high-concentration quality control blood spot are fixedly inserted into the second and third reagent tubes from the left of the first long side of the well plate, that is to say, the test values ​​of the second and third reagent tubes from the left of the first long side of the well plate should be about 10 and 30 respectively. If the well plate is placed incorrectly on the bottom plate, it will be found that the test values ​​of the second and third reagent tubes from the left of the first long side of the well plate are obviously low (such as 1.2 and 2.1, etc.), and the test values ​​of the second and third reagent tubes from the right of the second long side of the well plate are obviously high, which is consistent with the test results of the quality control product. It can be directly judged that the well plate is in the wrong position on the bottom plate, and the operator is reminded to correct it to the correct placement as soon as possible.

[0032] In some ways, such a structural design can also be used to determine whether there are errors caused by swapping or taking the wrong well plates in a batch of well plates arranged in sequence, such as the quality control sample of the first well plate is fixed at position A, the quality control sample of the second well plate is fixed at position B, the quality control sample of the third well plate is fixed at position C, etc. If it is found through testing that the test result of position A of the first well plate does not match the test result of the quality control product, but the test result of position B does match the test result of the quality control product, and the test result of position B of the second well plate does not match the test result of the quality control product, but the test result of position A does match the test result of the quality control product, then it can be determined that the first well plate and the second well plate are swapped. Other situations can be analogous.

[0033] The utility model has the following beneficial effects:

[0034] (1) It can provide timely warning of whether the orifice plate is placed correctly, reminding the operator to correct the correct placement as soon as possible, thereby avoiding subsequent problems caused by the wrong placement of the orifice plate on the base plate;

[0035] (2) Simple structure and easy to use;

[0036] (3) Provides a practical guarantee for the correct placement of the orifice plate on the base plate, which is suitable for large-scale promotion and application;

[0037] (4) Ensure that the quality of screening projects is controllable and the experimental results are traceable. BRIEF DESCRIPTION OF THE DRAWINGS

[0038] Figure 1 A structural diagram of the orifice plate device with a position error prevention function provided in Example 1;

[0039] Figure 2 A bottom view of the orifice plate provided in Example 1;

[0040] Figure 3 A schematic diagram of the structure of the base plate provided in Example 1;

[0041] Figure 4 A schematic diagram showing that the orifice plate with the function of preventing position errors provided in Example 1 is correctly placed on the bottom plate;

[0042] Figure 5 A schematic diagram showing that the orifice plate provided in Example 1 is correctly placed on the bottom plate and can be identified by a barcode scanner;

[0043] Figure 6 A schematic diagram showing that the orifice plate provided in Example 1 is placed incorrectly on the bottom plate;

[0044] Figure 7 A schematic diagram showing that the orifice plate provided in Example 1 is placed incorrectly on the bottom plate and cannot be recognized by the barcode scanner;

[0045] Figure 8 The test results of the quality control samples when the well plate provided in Example 2 is placed incorrectly;

[0046] Fig. 9 Schematic diagram of the situation in which the first orifice plate and the second orifice plate provided in Example 2 are swapped. DETAILED DESCRIPTION

[0047] The present invention is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be pointed out that the embodiments described below are intended to facilitate the understanding of the present invention and do not have any limiting effect on the present invention.

[0048] Embodiment 1: The orifice plate device provided by the utility model has the function of preventing position errors

[0049] The structure of the orifice plate device with the function of preventing position error provided in this embodiment is as follows Figure 1 As shown; the bottom view of the orifice plate is as shown Figure 2 As shown; the structural diagram of the bottom plate is as follows Figure 3 As shown; the schematic diagram of the correct placement of the orifice plate on the bottom plate is as follows Figure 4 As shown; the schematic diagram of the orifice plate being placed incorrectly on the bottom plate is as follows Figure 5 As shown; the schematic diagram of the orifice plate being correctly placed on the base plate and being recognized by the barcode scanner is as follows Figure 6 As shown in the figure; the orifice plate is placed incorrectly on the bottom plate and cannot be recognized by the barcode scanner as shown in the figure Figure 7 shown.

[0050] like Figure 1 The orifice plate device 1 with the function of preventing position error provided in this embodiment includes an orifice plate 2 and a bottom plate 3, a protrusion 4 is provided at one corner of the bottom plate 3, and a convex ridge 5 is provided at one corner of the orifice plate 2; when the orifice plate 2 is placed incorrectly on the bottom plate 3, the convex ridge 5 on the orifice plate 2 meets the protrusion 4 on the bottom plate 3, making it impossible for the orifice plate 2 to be placed flat on the bottom plate 3.

[0051] like Figure 2 , a reagent tube 6 is provided in the well plate 2, but there is no reagent tube 6 at the four corners of the well plate 2. Therefore, the corners of the well plate 2 without ridges 5 are in a suspended state. Even if they are placed on the protrusions 4, they can be smoothly laid flat on the protrusions 4. However, the corners with ridges 5 will be lifted up because the ridges 5 hit the protrusions 4, and thus cannot be laid flat.

[0052] Preferably, a rectangular flat panel 47 having a size matching that of the orifice plate 2 is provided on the bottom plate 3, and the orifice plate 2 can be completely placed above the bottom plate 3. The bottom plate 3 is provided with a rectangular flat panel 47 having a size matching that of the orifice plate 2, which means that the size of the rectangular flat panel 47 of the bottom plate 3 is consistent with that of the orifice plate 2 or is slightly larger than that of the orifice plate 2, so that the orifice plate 2 can be stably placed on the bottom plate 3 for the next step of operation. In actual operation, one or more rectangular flat panels 47 can be provided on the bottom plate 3, so that multiple orifice plates 2 can be placed, such as 1, 2, 4, 6 orifice plates 2, etc. This embodiment is described for a bottom plate 3 having only 1 rectangular panel 47, so that only one orifice plate 2 is placed, and other situations can be deduced by analogy.

[0053] Since the bottom plate 3 and the orifice plate 2 are both rectangular, when the orifice plate 2 is placed on the bottom plate 3, the long side of the orifice plate 2 is parallel to the long side of the bottom plate 3, and the short side of the orifice plate 2 is parallel to the short side of the bottom plate 3 (the long side of the bottom plate described in this embodiment is the long side of the rectangular plane plate, and the short side of the bottom plate is the short side of the rectangular plane plate). However, the orifice plate 2 and the bottom plate 3 both have two long sides and two short sides. When placed correctly, the first long side 7 of the orifice plate 2 must be close to the first long side 8 of the bottom plate, and the second long side 9 of the orifice plate 2 must be close to the second long side 10 of the bottom plate 3. At this time, the first short side 11 of the orifice plate 2 is also close to the first short side 12 of the bottom plate 3, and the second short side 13 of the orifice plate 2 is also close to the second short side 14 of the bottom plate 3. In other words, the orifice plate 2 can be placed directly on the bottom plate 3 according to the shape of the bottom plate 3, and the long side and the short side cannot be placed wrongly, and the long side of the orifice plate 2 cannot be placed on the short side of the bottom plate 3, which obviously cannot be placed. At the same time, the orifice plate 2 has a front and back side, and when in use, the front side 15 must face upward, that is, the opening 16 of the reagent tube 6 in the orifice plate 2 faces upward. In actual operation, it is impossible to place the back side 17 of the orifice plate 2 upward due to placement error, which makes subsequent testing impossible. Therefore, there are only two possible ways to place the orifice plate 2 on the bottom plate 3. The first is the correct placement: the first long side 7 of the orifice plate 2 is close to the first long side 8 of the bottom plate 3, and the second long side 9 of the orifice plate 2 is close to the second long side 10 of the bottom plate 3 ( Figure 4 ); The second is an incorrect placement: the first long side 7 of the orifice plate 2 is close to the second long side 10 of the bottom plate 3, and the second long side 9 of the orifice plate 2 is close to the first long side 8 of the bottom plate 3 ( Figure 5 ). And the second wrong placement is also very easy to occur. Once due to an operational error, the orifice plate 2 is placed on the base plate 3 in the second wrong placement, it is difficult for the operator to find it, which will cause each sample detected on the orifice plate 2 to not correspond to the test result, thereby seriously affecting the results of the screening of genetic metabolic diseases in newborns. There must be an early warning method to remind the operator to correct it immediately. In this embodiment, the orifice plate 2 and the base plate 3 are structurally improved. When the orifice plate 2 is placed on the base plate 3 in the second wrong placement, one end 18 of the orifice plate 2 will be raised and cannot be laid flat, thereby giving an early warning of the wrong placement and reminding the operator to correct it to the correct placement as soon as possible (equivalent to rotating the orifice plate 2 horizontally 180° and then re-placing it).

[0054] The shapes of the ridges 5 and the protrusions 4 can be any shapes, such as triangular prisms, cylinders, vertical plates, triangular pyramids, cones, etc. The shapes of the ridges 5 and the protrusions 4 can be the same or different, as long as the orifice plate 2 cannot be laid flat on the bottom plate 3 when the ridges 5 and the protrusions 4 meet, and the orifice plate 2 can be laid flat on the bottom plate 3 when they do not meet.

[0055] like Figure 1 When the orifice plate 2 is correctly placed on the bottom plate 3, the corner 19 with the ridge on the orifice plate 2 and the corner 20 with the protrusion on the bottom plate 3 are respectively located at the diagonal position of the orifice plate 2 or the bottom plate 3; however, when the orifice plate 2 is incorrectly placed on the bottom plate 3, the corner 19 with the ridge on the orifice plate 2 and the corner 20 with the protrusion on the bottom plate 2 are located at the same position, and the ridge 5 is located above the protrusion 4, so that the orifice plate 2 is raised. In order to make the ridge 5 of the orifice plate 2 meet the protrusion 4 of the bottom plate 3 when the orifice plate 2 is incorrectly placed, the ridge 5 of the orifice plate 2 and the protrusion 4 of the bottom plate 3 must be in a diagonal position when the orifice plate 2 is correctly placed. For example, when the convex ridge 5 of the orifice plate 2 is arranged at the left corner 21 of the second long side 9 of the orifice plate 2, and the protrusion 4 of the bottom plate 3 is arranged at the right corner 22 of the first long side 8 of the bottom plate 3, once an incorrect placement occurs, the orifice plate 2 is rotated horizontally by 180°, and the left corner 21 of the second long side 9 of the orifice plate 2 will be transferred to the right corner 22 of the first long side 8 of the bottom plate 3, so that the convex ridge 5 of the orifice plate 2 meets the protrusion 4 of the bottom plate 3, and the orifice plate 2 cannot be laid flat; for another example, when the convex ridge 5 of the orifice plate 2 is arranged at the left corner 23 of the first long side 7 of the orifice plate 2, and the protrusion 4 of the bottom plate 3 is arranged at the right corner 24 of the second long side 10 of the bottom plate 3, once an incorrect placement occurs, the orifice plate 2 is rotated horizontally by 180°, and the left corner 23 of the first long side 7 of the orifice plate 2 will be transferred to the right corner 24 of the second long side 10 of the bottom plate 3, so that the convex ridge 5 of the orifice plate 2 meets the protrusion 4 of the bottom plate 3, and the orifice plate 2 cannot be laid flat.

[0056] It can be understood that there are four such settings. The ridge 5 of the orifice plate 2 can be set at the left corner 23①, the right corner 25② of the first long side 7 of the orifice plate 2, the left corner 21③, and the right corner 26④ of the second long side 9. The corresponding protrusions 4 of the bottom plate 3 must be set at the right corner 24①, the left corner 27② of the second long side 10, the right corner 22③, and the left corner 28④ of the first long side 8. It only needs to be ensured that the corner with the ridge 5 on the orifice plate 2 and the corner with the protrusion 4 on the bottom plate 3 are respectively located at the two corners corresponding to the diagonal lines of the orifice plate 2 or the bottom plate 3.

[0057] Preferably, the well plate 2 can be any one or more of a 32-well plate, a 96-well plate, a 384-well plate, etc. In this embodiment, the well plate 2 is a 96-well plate 29, and a rectangular flat plate 47 having the same size as the 96-well plate 29 is provided on the bottom plate 3. Figures 1 to 3 The four corners on the 96-well plate 29 are respectively A1 corner 30, A12 corner 31, H1 corner 32, and H12 corner 33, which should be placed above the four corners A1' corner 34, A12' corner 35, H1' corner 36, and H12' corner 37 of the bottom plate 3; the H1 corner 32 of the 96-well plate 29 is provided with a convex ridge 5, and the A12' corner 35 of the bottom plate 3 is provided with a protrusion 4.

[0058] Preferably, Figure 6 , a barcode 38 is provided on one side of the orifice plate 2, and a barcode scanner 39 is provided on one side of the bottom plate 3. When the orifice plate 2 is placed correctly on the bottom plate 3, the barcode 38 on the orifice plate 2 can be recognized by the barcode scanner 39, and when the orifice plate 2 is placed incorrectly on the bottom plate 3, the barcode scanner 39 cannot scan the barcode 38 ( Figure 7 ).

[0059] like Figure 6 The barcode 38 is located on the side wall 40 of the orifice plate 2, and the barcode scanner 39 is located on one side of the bottom plate 3, and can be located on the bottom plate 3 or outside the bottom plate 3 ( Figure 3 The barcode 38 is located within the scanning range of the barcode scanner 39. Preferably, the barcode 38 is printed on the vertical side wall 40 of the first long side 7 of the orifice plate 2, and a barcode scanner 39 is provided on the outer side of the first long side 8 close to the bottom plate 3. When the orifice plate 2 is correctly placed on the bottom plate 3 (the first long side 7 of the orifice plate 2 is close to the first long side 8 of the bottom plate 3, and the second long side 9 of the orifice plate 2 is close to the second long side 10 of the bottom plate 3), the barcode 38 is located within the scanning range of the barcode scanner 39 and can be recognized by the barcode scanner 39; and when the orifice plate 2 is incorrectly placed on the bottom plate 3 (the first long side 7 of the orifice plate 2 is close to the second long side 10 of the bottom plate 3, and the second long side 9 of the orifice plate 2 is close to the first long side 8 of the bottom plate), the barcode 38 cannot be recognized by the barcode scanner 39 because it is on the other side ( Figure 7 ), the operator will be reminded to correct the placement as soon as possible.

[0060] Preferably, Figure 1 , in the reagent tubes 6 of the well plate 2, the reagent tubes 6 at specific positions are selected to fix the quality control samples. The reagent tubes 6 at one or more specific positions in the well plate 2 can be selected to fix the quality control samples, or the reagent tubes 6 at one or more specific positions in the well plate 2 can be selected to fix the low-concentration quality control samples 41 or the high-concentration quality control samples 42, or the reagent tubes 6 at two specific positions in the well plate 2 can be selected to fix the low-concentration quality control samples 41 or the high-concentration quality control samples 42. By fixing the quality control products at specific positions in the well plate 2, it can also help to warn of the wrong placement of the well plate.

[0061] There are two kinds of dry blood spots placed in the well plate 2 for testing, one is the neonatal blood spot to be tested (or called the sample to be tested), and the other is the quality control blood spot (low and high concentrations each occupy one well). The test value of the sample to be tested is unknown, but is generally in a relatively low level range, for example, around the single digit; the quality control blood spot generally has two concentration levels, for example, the low concentration is roughly around 10, and the high concentration level is roughly around 30. In general, the test value of the quality control product will be significantly different from that of the sample to be tested. Based on this feature, by fixing the quality control blood spot in a specific hole in the well plate 2, it is possible to judge whether the position of the well plate 2 is correctly placed based on the test results. For example, it is stipulated that a low-concentration quality control sample 41 (low-concentration quality control blood spot) and a high-concentration quality control sample 42 (high-concentration quality control blood spot) are fixedly injected into the second reagent tube 43 (A02 hole position) and the third reagent tube 44 (A03 hole position) from the left of the first long side 7 of the well plate 2, respectively. That is to say, the detection values ​​of the second reagent tube 43 and the third reagent tube 44 from the left of the first long side 7 of the well plate 2 should be about 10 and 30, respectively. If the well plate 2 is placed incorrectly on the bottom plate 3, it will be found that the detection values ​​of the second reagent tube 43 and the third reagent tube 44 from the left of the first long side 7 of the well plate 2 are obviously low (for example, 1.2 and 2.1, etc.), and the detection values ​​of the second reagent tube 45 (H11 hole position) and the third reagent tube 46 (H10 hole position) from the right of the second long side 9 of the well plate 2 are obviously high, which is consistent with the detection results of the quality control product. It can be directly judged that the position of the well plate 2 on the bottom plate 3 is wrong, and the operator is reminded to correct it to the correct placement as soon as possible. Such a structural design can also be used to determine whether a batch of orifice plates 2 arranged in sequence have errors caused by sequence arrangement errors or wrong selection, etc.

[0062] Embodiment 2: Method for early warning of orifice plate position error

[0063] When the orifice plate device 1 provided in Example 1 is used for the experiment, the following three methods can be used to determine whether the position of the orifice plate 2 on the bottom plate 3 is correct:

[0064] Method 1: Observe whether the orifice plate 2 can be placed stably

[0065] Align the long side of the orifice plate 2 with the long side of the bottom plate 3, and place the orifice plate 2 on the bottom plate 3. After placement, two situations will occur. The first one is as follows: Figure 3 , the orifice plate 2 can be placed horizontally and stably on the bottom plate 3, and no corner is tilted, which means the placement is correct; the second type is as follows Figure 5 If the orifice plate 2 cannot be placed horizontally on the bottom plate 3 and one corner is raised, it is judged that the placement is wrong and the orifice plate 2 needs to be rotated 180° horizontally and then placed again.

[0066] Method 2: Observe whether the barcode scanner 39 can recognize the barcode 38 on the well plate 2

[0067] Align the long side of the orifice plate 2 with the long side of the bottom plate 3, and place the orifice plate 2 on the bottom plate 3. After placement, two situations will occur. The first one is as follows: Figure 4 , the barcode 38 on the orifice plate 2 can be identified by the barcode scanner 39, and it is determined that the placement is correct; the second type is as follows Figure 5 , the barcode 38 on the orifice plate 2 is blocked and cannot be recognized by the barcode scanner 39, and it is judged that the placement is wrong. The orifice plate 2 needs to be rotated 180° horizontally and then rearranged.

[0068] Method 3: Judging by the test results

[0069] It is stipulated that the low-concentration quality control sample 41 (high-concentration quality control blood spot) and the high-concentration quality control sample 42 (low-concentration quality control blood spot) are fixedly injected into the A02 hole position 43 and the A03 hole position 44 of each well plate 2, and the samples to be tested are added into other reagent tubes 6 respectively, and the long side of the well plate 2 is aligned with the long side of the bottom plate 3, and the well plate 2 is placed on the bottom plate 3, and then the experimental test is directly carried out. There are two situations in the test results. The first one is that the experimental values ​​of the A02 hole position 43 and the A03 hole position 44 of the well plate 2 are about 10 and 30, and it is judged that the placement is correct; the second one is that the experimental values ​​of the A02 hole position 43 and the A03 hole position 44 of the well plate 2 are far from 10 and 30 (see Figure 8 ), are 1.3 and 2.2, then a significant anomaly is identified. Meanwhile, the experimental values ​​of H11 hole position 45 and H10 hole position 46 of orifice plate 2 are about 10 and 30, respectively. It is judged that the placement is wrong, and the orifice plate 2 needs to be rotated 180° horizontally and rearranged.

[0070] In addition, the third method can also be used to determine whether there is an error in the order of the orifice plates 2 in a batch.

[0071] Take a batch of well plates 2, and according to the differences in plate numbers (first well plate, second well plate, third well plate...), the low concentration quality control sample 41 is injected into the A02 well 43 of the first well plate 201, and the high concentration quality control sample 42 is injected into the A03 well 44; the low concentration quality control sample 41 is injected into the B02 well 47 of the second well plate 202, and the high concentration quality control sample 42 is injected into the B03 well 48; the low concentration quality control sample 41 is injected into the C02 well of the third well plate 203, and the high concentration quality control sample 42 is injected into the C03 well, and so on...

[0072] This embodiment only takes the case where the first orifice plate and the second orifice plate are exchanged as an example. Fig. 9, on the left is the first well plate 201, and on the right is the second well plate 202. The low-concentration quality control sample 41 is injected into the A02 well 43 of the first well plate 201, and the high-concentration quality control sample 42 is injected into the A03 well 44; the low-concentration quality control sample 41 is injected into the B02 well 47 of the second well plate 202, and the high-concentration quality control sample 42 is injected into the B03 well 48. During the experiment, the second orifice plate 202 was mistakenly used as the first orifice plate 201 for the experiment. After the test was completed, it was found that the experimental values ​​of A02 and A03 should be around 10 and 30, but now they are 1.3 and 2.2 (the values ​​are for example, for easy understanding), while the experimental values ​​of B02 and B03 should be in the single digit order of magnitude, but now they are 10 (in line with the low concentration quality control level) and 30 (in line with the high concentration quality control level), respectively. This makes it easy to identify significant abnormalities. According to the actual positions of the holes with the test value levels of 10 and 30, it can be inferred that in this case, the second orifice plate 202 was mistakenly used as the first orifice plate 201. Other situations can be analogized in the same way.

[0073] Although the present invention is disclosed as above, the present invention is not limited thereto. Any person skilled in the art can make various changes and modifications without departing from the spirit and scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the scope defined by the claims.

Claims

1. A hole plate device with a function of preventing position errors, characterized in that: The invention comprises a hole plate and a bottom plate, wherein a protrusion is arranged at one corner of the bottom plate and a convex edge is arranged at one corner of the hole plate; when the hole plate is placed incorrectly on the bottom plate, the convex edge on the hole plate meets the protrusion on the bottom plate, so that the hole plate cannot be laid flat.

2. The orifice plate device according to claim 1, characterized in that The well plate is provided with a reagent tube, but there are no reagent tubes at the four corners of the well plate, so the corners without ridges in the well plate are suspended below and can be smoothly laid flat on the protrusions, but the corners with ridges will be lifted up because the ridges hit the protrusions and cannot be laid flat.

3. The orifice plate device according to claim 2, characterized in that The bottom plate is provided with a rectangular plane plate whose size matches that of the orifice plate, and the orifice plate can be completely placed above the rectangular plane plate of the bottom plate.

4. The orifice plate device according to claim 3, characterized in that When the orifice plate is correctly placed on the base plate, the corner with the ridge on the orifice plate and the corner with the protrusion on the base plate are respectively located at two corners corresponding to the diagonal of the orifice plate or the base plate; however, when the orifice plate is incorrectly placed on the base plate, the corner with the ridge on the orifice plate and the corner with the protrusion on the base plate are located at the same position, and the ridge is located above the protrusion, causing the orifice plate to be partially raised.

5. The orifice plate device according to claim 4, characterized in that The well plate includes any one or more of a 32-well plate, a 96-well plate, and a 384-well plate.

6. The orifice plate device according to claim 5, characterized in that The well plate is a 96-well plate, and a rectangular plane plate having the same size as the 96-well plate is arranged on the bottom plate.

7. The orifice plate device according to claim 6, characterized in that The four corners on the 96-well plate are A1, A12, H1 and H12, which should be placed above the four corners of the bottom plate, A1', A12', H1' and H12', respectively; the H1 corner of the 96-well plate is provided with a convex ridge, and the A12' corner of the bottom plate is provided with a protrusion.

8. The orifice plate device according to claim 1, characterized in that A barcode is provided on one side of the orifice plate, and a barcode scanner is provided on one side of the bottom plate. When the orifice plate is placed correctly on the bottom plate, the barcode on the orifice plate can be recognized by the barcode scanner, but when the orifice plate is placed incorrectly on the bottom plate, the barcode scanner cannot scan the barcode.

9. The orifice plate device according to claim 8, characterized in that The barcode is located on the side wall of one side of the orifice plate, and the barcode scanner and one side of the bottom plate can be located on the bottom plate or outside the bottom plate, but the barcode must be located within the scanning range of the barcode scanner.

10. The orifice plate device according to claim 2, characterized in that: In the reagent tubes of the well plate, one or more reagent tubes are selected to fix the quality control samples.

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