Crushing device for cell culture

Abstract

The utility model relates to the technical field of crushing devices, and particularly discloses a crushing device for cell culture, which comprises a main body frame, an ultrasonic device arranged in the main body frame, a measuring cup arranged in the main body frame in a use state, and a detection assembly, the detection assembly is arranged at the bottom end of the working assembly and can detect water splashed by liquid in the measuring cup; the sensing assembly is connected to the bottom end of the detection assembly and can detect floating of the liquid surface of the measuring cup, information is transmitted to the ultrasonic device in the main body frame through the detection assembly, the power of the ultrasonic device is controlled, and water drops splashed from the liquid surface can be detected through the detection assembly of the device. Meanwhile, the sensing assembly is matched with the bottom end of the liquid level to make contact with the bottom end of the liquid level, whether the power of alternating water pressure intensity is too high or not can be judged through water waves on the liquid level, therefore, the overall power of the equipment can be controlled to be changed, the optimal cell breaking power is achieved, and the breaking process is more accurate.

Description

Technical Field

[0001] The utility model relates to the technical field of crushing devices, in particular to a crushing device for cell culture. Background Art

[0002] The cell disruption device is based on the cavitation effect produced by ultrasound in liquid. The piezoelectric transducer uses ordinary electrical energy through the horn to generate high-intensity shear force in the liquid at the top of the probe, forming a high-frequency and locally high-temperature alternating water pressure, which causes the bubble cavity to expand and explode instantly, thereby quickly crushing the entire cell wall. The cavitation effect of ultrasound on the fluid medium causes countless tiny cavities and bubbles to form in the liquid, which produce a strong pressure peak when they burst. At the same time, the shock wave generated by the "cavitation" effect of ultrasound acting on the liquid can achieve the purpose of dispersing the polymer.

[0003] However, the existing crushing device uses a control screen to control the high-frequency water pressure during use. However, for measuring cups of different specifications, there are different amounts of liquid and cells inside the measuring cups. In order to ensure the crushing of cells, different powers are required to generate alternating water pressure, which needs to be manually controlled within a fixed power range. However, the changes in different control powers will cause the liquid to vibrate with a large amplitude and cause liquid splashing due to the large adjustment data. For this reason, we propose a crushing device for cell culture. Utility Model Content

[0004] The utility model aims to provide a cell culture disruption device to solve the problem of liquid splashing mentioned in the above background technology.

[0005] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a cell culture disruption device, comprising a main frame, an ultrasonic device is arranged inside the main frame, a measuring cup is arranged inside the main frame when in use, and cells and liquid are arranged inside the measuring cup, a working component is arranged on the side of the main frame, and the working component can control the power of the ultrasonic device inside the main frame and control the opening of the device; and also includes a detection component, which is arranged at the bottom end of the working component and can detect the splash of liquid in the measuring cup;

[0006] The induction component is connected to the bottom of the detection component to detect the floating of the liquid surface of the measuring cup, and transmits the information to the ultrasonic device inside the main frame through the detection component to control its power.

[0007] Among them, the detection component includes a fixed frame, the top of the fixed frame is connected to the bottom of the working component, the inner wall of the fixed frame is slidably connected to a movable frame, the inner wall of the movable frame is fixedly connected to several groups of serrated plates, the inner wall of the movable frame is fixedly connected to a serrated plate, and the inner wall of the movable frame is fixedly connected to a sensing sheet.

[0008] The side of the fixed frame is rotatably connected with a knob, the bottom end of the movable frame is fixedly connected with a bottom plate, the side of the bottom plate is fixedly connected with a wire, and one end of the wire is fixedly connected with a controller.

[0009] Among them, the sensing component includes a connecting frame, the top of the connecting frame is fixedly connected to the bottom of the base plate, the inner wall of the connecting frame is rotatably connected to a rotating shaft, the side of the rotating shaft is fixedly connected to a pointer, the bottom end of the base plate is fixedly connected to a supporting frame, the inner wall of the supporting frame is fixedly connected to a sliding rheostat, the inner wall of the sliding rheostat is provided with a plurality of groups of resistor sheets, and the supporting frame and the sliding rheostat are arranged as an arc structure.

[0010] Wherein, two ends of the rotating shaft are fixedly connected with a tripod, and two ends of the tripod are fixedly connected with an air bag 1.

[0011] Wherein, two ends of the tripod are fixedly connected with airbags 2, and the airbags 2 are arranged in a square structure.

[0012] The working assembly comprises a door panel, the side of the door panel is rotatably connected to the side of the main frame, the inner wall of the main frame is fixedly connected to an ultrasonic probe, and the side of the main frame is fixedly connected to a control panel.

[0013] The utility model has at least the following beneficial effects:

[0014] The internal structure of the device can detect the vibration amplitude of the liquid inside the measuring cup. Compared with the existing technology, different control powers will cause large vibration amplitude of the liquid due to the large adjustment data. The detection component of the device can detect the water droplets splashed from the liquid surface, so as to judge whether the amplitude of the liquid surface vibration is too strong. At the same time, the sensing component is in contact with the bottom of the liquid surface, and the water ripples on the liquid surface can be used to judge whether the power of the alternating water pressure is too high, so that the overall power of the equipment can be controlled to change, so as to achieve the best power for crushing cells, reduce the control of personnel, make the crushing process more precise, and reduce power usage. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 It is a schematic diagram of the structure of the utility model;

[0016] Figure 2 This is a schematic diagram of the structure of the working components of the utility model;

[0017] Figure 3 This is a schematic diagram of the structure of the detection component of the utility model;

[0018] Figure 4 This is a schematic diagram of the structure of the induction sheet of the utility model;

[0019] Figure 5 This is a schematic diagram of the structure of the sensing component of the utility model;

[0020] Figure 6 This is a structural schematic diagram of the second embodiment of the utility model.

[0021] In the figure: 1. main frame; 2. working assembly; 3. detection assembly; 31. fixed frame; 32. movable frame; 33. serrated plate; 34. induction sheet; 35. knob; 36. bottom plate; 37. wire; 38. controller; 4. induction assembly; 41. connecting frame; 42. rotating shaft; 43. pointer; 44. supporting frame; 45. sliding rheostat; 46. tripod; 47. airbag one; 48. airbag two; 5. door panel; 6. ultrasonic probe; 7. control panel. DETAILED DESCRIPTION

[0022] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0023] Embodiment 1

[0024] See also Figure 1-6 The utility model provides a technical solution: a cell culture crushing device, comprising a main frame 1, an ultrasonic device is arranged inside the main frame 1, a measuring cup is arranged inside the main frame 1 when in use, and cells and liquid are arranged inside the measuring cup, a working component 2 is arranged on the side of the main frame 1, and the working component 2 can control the power of the ultrasonic device inside the main frame 1 and control the opening of the device; and also includes a detection component 3, which is arranged at the bottom end of the working component 2 and can detect the splash of liquid in the measuring cup;

[0025] The sensing component 4 is connected to the bottom end of the detection component 3 to detect the floating of the liquid surface of the measuring cup, and transmits the information to the ultrasonic device inside the main frame 1 through the detection component 3 to control its power.

[0026] The detection component 3 includes a fixed frame 31, the top of the fixed frame 31 is connected to the bottom of the working component 2, the inner wall of the fixed frame 31 is slidably connected with a movable frame 32, the inner wall of the movable frame 32 is fixedly connected with several groups of serrated plates 33, the inner wall of the movable frame 32 is fixedly connected with a serrated plate 33, and the inner wall of the movable frame 32 is fixedly connected with a sensing sheet 34. When the liquid inside the measuring cup vibrates too much, water droplets on the surface of the measuring cup will splash out due to the vibration, and the water splashes onto the surface of the serrated plate 33, and the surface tension of the serrated plate 33 will temporarily pull the water droplets to reduce the sliding speed of the water droplets on the surface of the movable frame 32. At the same time, the water droplets will connect the two groups of sensing sheets 34 and realize conductivity. By using the passage of induced current, the contact height of the sensing sheet 34 with the water droplets can be detected, thereby judging the height of the water droplets splashing, and then judging the intensity of the liquid surface vibration.

[0027] The side of the fixed frame 31 is rotatably connected to a knob 35, the bottom end of the movable frame 32 is fixedly connected to a base plate 36, the side of the base plate 36 is fixedly connected to a wire 37, one end of the wire 37 is fixedly connected to a controller 38, the knob 35 rotates inside the fixed frame 31 and presses against the side of the movable frame 32, so that the fixed frame 31 and the movable frame 32 are fixed, the height is detected by the sensor sheet 34 and transmitted through the base plate 36 and the wire 37, and the information is controlled to the controller 38, and then the controller 38 is used to control the overall water pressure power of the device.

[0028] The sensing component 4 includes a connecting frame 41, the top of which is fixedly connected to the bottom of the bottom plate 36, the inner wall of the connecting frame 41 is rotatably connected to a rotating shaft 42, the side of the rotating shaft 42 is fixedly connected to a pointer 43, the bottom end of the bottom plate 36 is fixedly connected to a supporting frame 44, and the inner wall of the supporting frame 44 is fixedly connected to a sliding rheostat 45. Since the interior of the airbag 47 adopts a hollow structure and the side of the airbag 47 is kept at the same height as the liquid surface, when water ripples appear on the liquid surface, the airbag 47 and the rotating shaft 42 will be driven to rotate inside the connecting frame 41, and the pointer 43 on the side of the rotating shaft 42 and the bottom end of the sliding rheostat 45 will slide. The resistor at the bottom end of the sliding rheostat 45 can be used to change the intensity of the current, and then the size of the water ripples can be detected according to the intensity of the current, thereby further judging the vibration intensity of the liquid surface of the measuring cup, and transmitting it to the controller 38 through the conduction of the bottom plate 36 and the wire 37.

[0029] The two ends of the rotating shaft 42 are fixedly connected with a tripod 46, and the two ends of the tripod 46 are fixedly connected with an air bag 47. The inside of the air bag 47 fits the liquid surface. Before use, the personnel need to adjust the relative position of the movable frame 32 and the fixed frame 31, and make the bottom plate 36 connected to the bottom end of the movable frame 32 keep the air bag 47 and the liquid surface at the same height, so as to realize the process of accurate detection.

[0030] Embodiment 2

[0031] The two ends of the tripod 46 are fixedly connected with airbag 2 48 , and the airbag 2 48 is set to a square structure. The square structure of the airbag 2 48 can increase the contact area with the liquid surface, so that the water pattern of the liquid surface can be detected more accurately.

[0032] The inner wall of the sliding rheostat 45 is provided with a plurality of groups of resistor sheets. The support frame 44 and the sliding rheostat 45 are arranged in an arc structure. By utilizing the rotation of the rotating shaft 42 on the surface of the pointer 43, the relative position between the pointer 43 and the rotating shaft 42 is changed, and then the resistance value of the current passing through the pointer 43 and the rotating shaft 42 is changed.

[0033] The working component 2 includes a door panel 5, the side of the door panel 5 is rotatably connected to the side of the main frame 1, an ultrasonic probe 6 is fixedly connected to the inner wall of the main frame 1, and a control panel 7 is fixedly connected to the side of the main frame 1. The bottom end of the ultrasonic probe 6 is inserted 1.5 centimeters below the liquid level of the measuring cup, and then the ultrasonic probe 6 is used to generate high-pressure shear force to break the cells.

[0034] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0035] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A cell culture disruption device, comprising a main frame (1), wherein an ultrasonic device is provided inside the main frame (1), wherein a measuring cup is provided inside the main frame (1) when in use, and cells and liquid are contained inside the measuring cup, wherein a working component (2) is provided on the side of the main frame (1), wherein the working component (2) can control the power of the ultrasonic device inside the main frame (1) and control the start of the device: characterized in that: It also includes a detection component (3), which is arranged at the bottom end of the working component (2) and can detect splashes of liquid in the measuring cup; The sensing component (4) is connected to the bottom end of the detection component (3) and can detect the floating of the liquid surface of the measuring cup, and transmit the information to the ultrasonic device inside the main frame (1) through the detection component (3) to control its power.

2. The cell culture disruption device according to claim 1, characterized in that: The detection component (3) comprises a fixed frame (31), the top end of the fixed frame (31) is connected to the bottom end of the working component (2), the inner wall of the fixed frame (31) is slidably connected to a movable frame (32), the inner wall of the movable frame (32) is fixedly connected to a plurality of groups of sawtooth plates (33), the inner wall of the movable frame (32) is fixedly connected to the sawtooth plates (33), and the inner wall of the movable frame (32) is fixedly connected to a sensing sheet (34).

3. The cell culture disruption device according to claim 2, characterized in that: The side of the fixed frame (31) is rotatably connected to a knob (35), the bottom end of the movable frame (32) is fixedly connected to a bottom plate (36), the side of the bottom plate (36) is fixedly connected to a wire (37), and one end of the wire (37) is fixedly connected to a controller (38).

4. The cell culture disruption device according to claim 1, characterized in that: The sensing component (4) comprises a connecting frame (41), the top end of the connecting frame (41) is fixedly connected to the bottom end of the base plate (36), the inner wall of the connecting frame (41) is rotatably connected to a rotating shaft (42), the side of the rotating shaft (42) is fixedly connected to a pointer (43), the bottom end of the base plate (36) is fixedly connected to a supporting frame (44), the inner wall of the supporting frame (44) is fixedly connected to a sliding rheostat (45), the inner wall of the sliding rheostat (45) is provided with a plurality of groups of resistor sheets, and the supporting frame (44) and the sliding rheostat (45) are arranged in an arc structure.

5. The cell culture disruption device according to claim 4, characterized in that: The two ends of the rotating shaft (42) are fixedly connected to a tripod (46), and the two ends of the tripod (46) are fixedly connected to an air bag 1 (47).

6. The cell culture disruption device according to claim 5, characterized in that: Two ends of the tripod (46) are fixedly connected to airbag 2 (48), and the airbag 2 (48) is configured as a square structure.

7. The cell culture disruption device according to claim 1, characterized in that: The working assembly (2) comprises a door panel (5), the side of the door panel (5) being rotatably connected to the side of the main frame (1), the inner wall of the main frame (1) being fixedly connected to an ultrasonic probe (6), and the side of the main frame (1) being fixedly connected to a control panel (7).

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