Detection box

Abstract

The detection kit comprises a reaction tank, a negative pressure tank, a first flow channel and a flow channel switch, the negative pressure tank is connected with a negative pressure device, a liquid storage tank communicated with the negative pressure tank is arranged in the negative pressure tank, the first flow channel is communicated with the reaction tank and the liquid storage tank, the flow channel switch is arranged in the first flow channel, and the flow channel switch is used for controlling connection or disconnection of the first flow channel. When the negative pressure pool is in a negative pressure state, the flow channel switch conducts the first flow channel, and liquid in the reaction pool enters the liquid storage tank through the first flow channel, so that the liquid is prevented from being sucked out by the negative pressure device. The negative pressure pool and the liquid storage tank communicated with the negative pressure pool are arranged, negative pressure is applied to the negative pressure pool, liquid can be conveniently and efficiently collected into the liquid storage tank, loss caused by the fact that the liquid is extracted by a negative pressure device in the negative pressure extraction process is avoided, the extraction efficiency is improved, follow-up detection is facilitated, and meanwhile the extraction efficiency is improved. As the liquid storage tank is arranged to isolate the liquid to be detected from the external environment, pollution can be avoided, and the detection precision is improved.

Description

Technical Field

[0001] The present disclosure relates to the technical field of biological gene detection, and in particular to a detection kit. Background Art

[0002] In the process of genetic testing, samples usually need to be processed through processes such as lysis, impurity removal, and filtration to obtain the test liquid containing the required substances for testing. However, the test liquid after processing is often difficult to collect, and there is a lot of loss during the collection and transfer process. It is also easily contaminated by the external environment, which indirectly leads to reduced detection efficiency and accuracy. Utility Model Content

[0003] In order to overcome the above-mentioned shortcomings of the prior art, the present disclosure aims to provide a detection box.

[0004] In order to achieve the above objectives, the present invention adopts the following technical solutions:

[0005] A detection kit, comprising:

[0006] Reaction tank;

[0007] A negative pressure pool connected to the negative pressure device, wherein the negative pressure pool is provided with a liquid storage tank connected to the negative pressure pool;

[0008] A first flow channel, connecting the reaction pool and the liquid storage tank;

[0009] A flow channel switch, disposed in the first flow channel, and used to control the conduction or cutoff of the first flow channel;

[0010] Among them, when the negative pressure pool is in a negative pressure state, the flow channel switch switches on the first flow channel, and the liquid in the reaction pool enters the liquid storage tank through the first flow channel to prevent the liquid from being sucked out by the negative pressure device.

[0011] In a possible implementation, the detection box further includes a second flow channel, and the second flow channel is connected to the reaction pool and the negative pressure pool respectively;

[0012] Wherein, when the negative pressure pool is in a negative pressure state, the flow channel switch cuts off the first flow channel, and the waste liquid in the reaction pool enters the second flow channel.

[0013] In a possible implementation, the detection box further includes a waste liquid pool and a third flow channel, and the third flow channel is connected to the second flow channel and the waste liquid pool respectively;

[0014] Wherein, when the negative pressure pool is in a negative pressure state, the flow channel switch cuts off the first flow channel, and the waste liquid in the reaction pool enters the third flow channel through the second flow channel and then enters the waste liquid pool through the third flow channel.

[0015] In a possible implementation, the liquid storage tank is connected to the flow channel switch via a cover plate, a fourth flow channel is provided inside the cover plate, and the fourth flow channel is connected to the first flow channel via the flow channel switch.

[0016] In a possible implementation, the detection box further includes a detection portion, a fifth flow channel is provided at the bottom of the liquid storage tank, and the liquid storage tank is connected to the detection portion through the fifth flow channel.

[0017] In a possible implementation, a guide surface is provided at the bottom of the liquid storage tank, one end of the guide surface is connected to the inner wall of the liquid storage tank, and the other end of the guide surface is connected to the fifth flow channel.

[0018] In a possible implementation, the guide surface includes an inclined plane or an inclined curved surface.

[0019] In a possible implementation, the guide surface includes a conical surface, the bottom surface of the conical surface is connected to the inner wall of the liquid storage tank, and the vertex of the conical surface is connected to the fifth flow channel.

[0020] In a possible implementation, the detection box further includes a box body, and the reaction pool and the negative pressure pool are both arranged inside the box body.

[0021] In a possible implementation, a plurality of reagent pools are further disposed inside the box body and are distributed around the reaction pool, and each of the reagent pools is connected to the reaction pool.

[0022] The advantages of the present invention are as follows: the present invention provides a negative pressure pool and a liquid storage tank connected to the negative pressure pool, and by applying negative pressure to the negative pressure pool, the liquid can be conveniently and efficiently collected in the liquid storage tank without being extracted by the negative pressure device during the negative pressure extraction process, resulting in loss, thereby improving the extraction efficiency and facilitating subsequent detection. At the same time, since the liquid storage tank is provided to isolate the liquid to be detected from the external environment, contamination can also be avoided and the detection accuracy can be improved.

[0023] Other features and advantages of the present disclosure will be set forth in the subsequent description, and in part will become apparent from the description, or may be understood through implementation of the present application. The objects and other advantages of the present application may be realized and obtained through the structures particularly pointed out in the description, claims and drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] The accompanying drawings, which are incorporated into the specification and constitute a part of the specification, illustrate embodiments of the present disclosure and are used together with the description to explain the principles of the present disclosure. In these drawings, similar reference numerals are used to identify similar elements. The drawings described below are some embodiments of the present disclosure, rather than all embodiments. For those of ordinary skill in the art, other drawings can be obtained based on these drawings without paying creative work.

[0025] Figure 1 is one of the structural schematic diagrams of a detection box according to an exemplary embodiment.

[0026] Figure 2 This is a second schematic diagram of the structure of a detection box according to an exemplary embodiment.

[0027] Figure 3 This is a third schematic diagram of the structure of a detection box according to an exemplary embodiment.

[0028] Figure 4 is a top view of a detection box according to an exemplary embodiment.

[0029] Figure 5 yes Figure 4 Schematic diagram of the AA section.

[0030] Figure 6 is a front view of a detection box according to an exemplary embodiment.

[0031] Figure 7 yes Figure 6 Schematic diagram of the BB section. DETAILED DESCRIPTION

[0032] In order to make the purpose, technical scheme and advantages of the embodiments of the present disclosure clearer, the technical scheme in the embodiments of the present disclosure will be clearly and completely described below in conjunction with the drawings in the embodiments of the present disclosure. Obviously, the described embodiments are part of the embodiments of the present disclosure, rather than all of the embodiments. Based on the embodiments of the present disclosure, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present disclosure. It should be noted that, in the absence of conflict, the embodiments in this application and the feature vectors in the embodiments can be arbitrarily combined with each other.

[0033] In the process of genetic testing, samples usually need to be processed through processes such as lysis, impurity removal, and filtration to obtain the test liquid containing the required substances for testing. However, the test liquid after processing is often difficult to collect, and there is a lot of loss during the collection and transfer process. It is also easily contaminated by the external environment, which indirectly leads to reduced detection efficiency and accuracy.

[0034] In order to solve the above problems, the present disclosure provides a detection box, which is provided with a negative pressure pool and a liquid storage tank connected to the negative pressure pool. By applying negative pressure to the negative pressure pool, the liquid can be conveniently and efficiently collected in the liquid storage tank without being extracted by the negative pressure device during the negative pressure extraction process and causing loss, thereby improving the extraction efficiency and facilitating subsequent detection. At the same time, since the liquid storage tank is provided to isolate the liquid to be detected from the external environment, contamination can be avoided and the detection accuracy can be improved.

[0035] According to an exemplary embodiment, Figure 1-Figure 7 As shown, an embodiment of the present disclosure provides a detection box, which includes a reaction tank 10 and a negative pressure tank 20. The reaction tank 10 is used to hold samples that need to be processed, and a variety of reagents are added to the samples. After lysis, impurity removal, filtration and other processes, a liquid to be detected is obtained. The negative pressure tank 20 is connected to a negative pressure device (not shown in the figure), and the negative pressure device can be, for example, a vacuum pump. A liquid storage tank 21 connected to the negative pressure tank 20 is provided in the negative pressure tank 20, and the liquid storage tank 21 is used to collect and store the liquid to be detected. One end of the first flow channel 31 is connected to the reaction tank 10, and the other end of the first flow channel 31 is connected to the liquid storage tank 21, thereby connecting the reaction tank 10 and the liquid storage tank 21, so that the liquid in the reaction tank 10 can enter the liquid storage tank 21 through the first flow channel 31.

[0036] The detection box further includes a flow channel switch 301, which is disposed in the first flow channel 31 and is used to control the conduction or cutoff of the first flow channel 31. When the flow channel switch 301 conducts the first flow channel 31 and the negative pressure tank 20 is in a negative pressure state, the liquid in the reaction tank 10 (that is, the liquid to be detected obtained after treatment) enters the liquid storage tank 21 through the first flow channel 31.

[0037] In the disclosed embodiment, a negative pressure pool and a liquid storage tank connected to the negative pressure pool are provided. By applying negative pressure to the negative pressure pool, the liquid to be tested can be conveniently and efficiently collected in the liquid storage tank without being extracted by the negative pressure device during the negative pressure extraction process, resulting in loss of the liquid to be tested. This improves the extraction efficiency and facilitates subsequent testing. At the same time, since the liquid storage tank is provided to isolate the liquid to be tested from the external environment, contamination of the liquid to be tested can also be avoided, thereby improving detection accuracy.

[0038] In some embodiments, the detection box further includes a second flow channel. One end of the second flow channel is connected to the reaction pool 10, and the other end of the second flow channel is connected to the negative pressure pool 20, thereby connecting the reaction pool 10 and the negative pressure pool 20. Among them, when the flow channel switch 301 cuts off the first flow channel 31, and when the negative pressure pool 20 is under negative pressure, the waste liquid in the reaction pool 10 flows out of the reaction pool 10. As an example, when the amount of waste liquid is small, the waste liquid is drawn into the second flow channel by the negative pressure. Since the amount of waste liquid is small, there is no need to collect the waste liquid separately, and a small amount of waste liquid can be temporarily stored in the second flow channel.

[0039] In some embodiments, the detection box further includes a waste liquid pool 22 and a third flow channel. Figure 6-Figure 7 As shown, the waste liquid pool 22 is arranged inside the box body, one end of the third flow channel is connected to the second flow channel, and the other end of the third flow channel is connected to the waste liquid pool 22. Among them, when the flow channel switch 301 cuts off the first flow channel 31 and the negative pressure pool 20 is in a negative pressure state, the waste liquid in the reaction pool 10 flows out of the reaction pool 10 into the second flow channel, and then the waste liquid enters the third flow channel through the second flow channel, and then enters the waste liquid pool 22 through the third flow channel. In addition, the waste liquid pool 22 can be connected to other negative pressure devices to make the waste liquid flow in the direction of the waste liquid pool 22, and other flow channel switches can be set to control the blocking between the second flow channel and the negative pressure pool 20, so that the waste liquid flows in the direction of the waste liquid pool 22. Of course, it is understandable that the waste liquid pool 22 is set up to collect the waste liquid only when the amount of waste liquid is large or other treatment processes are required for the waste liquid, such as harmless treatment. If the amount of waste liquid is small and no additional treatment of the waste liquid is required, the waste liquid pool 22 may not be set up, and the waste liquid may only remain in the second flow channel, or remain in the second flow channel and the third flow channel.

[0040] In some embodiments, the negative pressure pool 20 is connected to a negative pressure device. The negative pressure device is used to generate negative pressure, thereby attracting the liquid to be tested or the waste liquid to flow out of the reaction pool 10. In one example, the negative pressure device is a small negative pressure device arranged inside the detection box, such as a micro vacuum pump. In another example, Figure 7 As shown, the negative pressure device is an independent negative pressure device arranged outside the detection box, such as an air pump, etc. The negative pressure device arranged outside the detection box is connected to the negative pressure pool 20 through the negative pressure channel 36 to generate negative pressure, which produces a traction effect on the liquid to be detected and the waste liquid.

[0041] In some embodiments, the liquid storage tank 21 is connected to the flow channel switch 301 through the cover plate 302. Figure 3 As shown, a fourth flow channel (not shown in the figure) is arranged inside the cover plate 302, and the fourth flow channel is connected to the first flow channel 31 through the flow channel switch 301. Since the cover plate 302 is arranged, the liquid storage tank 21 is directly connected to the flow channel switch 301, which improves the collection efficiency of the liquid to be detected. In addition, since the fourth flow channel is arranged inside the cover plate 302, the cover plate 302 can play a good sealing and protective role, preventing the liquid to be detected from being contaminated by the external environment when it is collected from the reaction pool 10 into the liquid storage tank 21, which is conducive to improving the detection accuracy and detection reliability.

[0042] In some embodiments, the detection box further includes a detection unit 40. A fifth flow channel is provided at the bottom of the liquid storage tank 21, one end of the fifth flow channel is connected to the liquid storage tank 21, and the other end of the fifth flow channel is connected to the detection unit 40, so that the liquid storage tank 21 is connected to the detection unit 40. The detection unit 40 is used to perform at least one test on the liquid to be detected flowing into the detection unit 40. For example, a detection reagent and an optical detection component can be provided inside the detection unit 40. After the detection reagent reacts with the liquid to be detected, the optical detection component performs detection and spectral analysis to obtain a detection result.

[0043] In some embodiments, a guide surface 211 is disposed inside the liquid storage tank 21. Figure 4-Figure 7 As shown, the guide surface 211 is arranged at the bottom of the liquid storage tank 21, one end of the guide surface 211 is connected to the inner wall of the liquid storage tank 21, and the other end of the guide surface 211 is provided with a guide hole 212, which is connected to the fifth flow channel. The guide surface 211 is used to guide the liquid to be detected collected in the liquid storage tank 21, so that the liquid to be detected can enter the fifth flow channel along the guide surface 211 under the action of gravity, and enter the detection part 40 through the fifth flow channel. Among them, the guide surface 211 can be a smooth surface to reduce the friction of the surface, increase the speed at which the liquid to be detected enters the detection part 40, and reduce the residue of the liquid to be detected on the guide surface 211.

[0044] In some embodiments, as shown in FIG. Figure 4-Figure 7 As shown, the guide surface 211 includes an inclined plane or an inclined curved surface. In one example, the guide surface 211 includes an inclined plane, the upper end of the inclined plane is connected to the inner wall of the liquid storage tank 21, and the lower end of the inclined plane is provided with a guide hole 212, and the guide hole 212 is connected to the fifth flow channel, so that the liquid to be detected can enter the fifth flow channel along the guide surface 211. In another example, the guide surface 211 includes an inclined curved surface, the upper end of the inclined curved surface is connected to the inner wall of the liquid storage tank 21, and the lower end of the inclined curved surface is connected to the fifth flow channel. The inclined curved surface can be a convex surface or a convex surface, and the curvature can also be adjusted according to actual needs to change the speed at which the liquid to be detected flows along the guide surface 211.

[0045] Of course, it is understandable that the guide surface 211 may also include multiple sections of connected inclined planes and / or inclined curved surfaces. The multiple sections of inclined planes and / or inclined curved surfaces may be connected in sequence along the depth direction of the liquid storage tank 21, or may be connected in sequence along the circumference of the inner wall of the liquid storage tank 21. Those skilled in the art may adjust the shape, angle and other parameters of the guide surface 211 according to actual needs to adjust the flow speed of the liquid to be tested along the guide surface 211, and the embodiments of the present disclosure do not impose too many restrictions on this.

[0046] In one example, the guide surface 211 includes a conical surface. The bottom surface of the conical surface is connected to the inner wall of the liquid storage tank 21, and the vertex of the conical surface is connected to the fifth flow channel. Since the guide surface 211 is set as a conical surface, the liquid to be detected can easily converge at the vertex of the conical surface under the action of gravity and the guidance of the guide surface 211 after entering the liquid storage tank 21, thereby facilitating the collection of the liquid to be detected.

[0047] In some embodiments, the detection box further includes a box body 60, and the reaction pool 10 and the negative pressure pool 20 are both disposed inside the box body 60. A plurality of reagent pools 50 are also disposed inside the box body 60, and the plurality of reagent pools 50 are distributed around the circumference of the reaction pool 10, and each reagent pool 50 is connected to the reaction pool 10. The plurality of reagent pools 50 are used to store reagents, and the types of the reagents may be the same or different, so as to facilitate the sample in the reaction pool 10 to be processed in batches, thereby better extracting the liquid to be detected from the sample.

[0048] The box body 60 is used to provide sealing protection for the reaction pool 10, the negative pressure pool 20, the reagent pool 50 and each flow channel of the detection box to prevent damage from collision or foreign impurities from entering and affecting the detection results. The material of the box body 60 can be polyethylene, polypropylene, polycarbonate and the like, and the materials of the reaction pool 10, the negative pressure pool 20 and the reagent pool 50 can be the same or different, as long as the selected materials are chemically stable and will not react with the reagents, samples and the liquid to be detected to affect the detection results.

[0049] In some embodiments, the detection box may include multiple reaction pools. For example, the reaction pool 10 includes a first reaction pool 11 and a second reaction pool 12, the first reaction pool 11 and the second reaction pool 12 are connected, and multiple reagent pools 50 are arranged around the first reaction pool 11 and the second reaction pool 12. The first reaction pool 11 is used to add the initial sample, and after preliminary treatment, an easy-to-operate sample is obtained. The sample enters the second reaction pool 12 for multiple treatments and obtains the final liquid to be tested. Among them, the first reaction pool 11 can be provided with a sealing plug 111, and the sealing plug 111 can include flexible materials such as silicone and rubber. The sealing plug 111 seals the first reaction pool 11 after the initial sample is added to avoid external contamination that affects the detection accuracy.

[0050] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device comprising a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device. In the absence of further restrictions, the elements defined by the statement "include..." do not exclude the existence of other identical elements in the process, method, article or device comprising the elements.

[0051] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present disclosure rather than to limit it. The present disclosure is described in detail with reference to the preferred embodiments only. Those skilled in the art should understand that the technical solution of the present disclosure can be modified or replaced by equivalents without departing from the spirit and scope of the technical solution of the present disclosure, and should be included in the scope of the claims of the present disclosure.

Claims

1. A detection kit, characterized in that: include: Reaction tank; A negative pressure pool connected to the negative pressure device, wherein the negative pressure pool is provided with a liquid storage tank connected to the negative pressure pool; A first flow channel, connecting the reaction pool and the liquid storage tank; A flow channel switch, disposed in the first flow channel, and used to control the conduction or cutoff of the first flow channel; Among them, when the negative pressure pool is in a negative pressure state, the flow channel switch switches on the first flow channel, and the liquid in the reaction pool enters the liquid storage tank through the first flow channel to prevent the liquid from being sucked out by the negative pressure device.

2. The detection kit according to claim 1, characterized in that: The detection box further comprises a second flow channel, wherein the second flow channel is connected to the reaction pool and the negative pressure pool respectively; Wherein, when the negative pressure pool is in a negative pressure state, the flow channel switch cuts off the first flow channel, and the waste liquid in the reaction pool enters the second flow channel.

3. The detection kit according to claim 2, characterized in that: The detection box also includes a waste liquid pool and a third flow channel, and the third flow channel is connected to the second flow channel and the waste liquid pool respectively; Wherein, when the negative pressure pool is in a negative pressure state, the flow channel switch cuts off the first flow channel, and the waste liquid in the reaction pool enters the third flow channel through the second flow channel and then enters the waste liquid pool through the third flow channel.

4. The detection kit according to claim 1, characterized in that: The liquid storage tank is connected to the flow channel switch through a cover plate, a fourth flow channel is arranged inside the cover plate, and the fourth flow channel is connected to the first flow channel through the flow channel switch.

5. The detection kit according to claim 1, characterized in that: The detection box further includes a detection portion, a fifth flow channel is provided at the bottom of the liquid storage tank, and the liquid storage tank is connected to the detection portion through the fifth flow channel.

6. The detection kit according to claim 5, characterized in that: A guide surface is arranged at the bottom of the liquid storage tank, one end of the guide surface is connected to the inner wall of the liquid storage tank, and the other end of the guide surface is connected to the fifth flow channel.

7. The detection kit according to claim 6, characterized in that: The guide surface includes an inclined plane or an inclined curved surface.

8. The detection kit according to claim 6, characterized in that: The guide surface includes a conical surface, the bottom surface of the conical surface is connected to the inner wall of the liquid storage tank, and the vertex of the conical surface is connected to the fifth flow channel.

9. The detection kit according to claim 1, characterized in that: The detection box also includes a box body, and the reaction pool and the negative pressure pool are both arranged inside the box body.

10. The detection box according to claim 9, characterized in that: A plurality of reagent pools are also arranged inside the box body and are distributed around the reaction pool in a circumferential direction, and each of the reagent pools is connected to the reaction pool.

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