Batch pressurizing device for bottled cells

Abstract

The utility model discloses a batch pressurizing device for bottled cells, which comprises a closed box body with a cover body, an air inlet pipe orifice and a pressure measuring pipe orifice are arranged on the cover body, and the air inlet pipe orifice and the pressure measuring pipe orifice are connected with external pressure control equipment and pressure measuring equipment through sealing joints. A liquid medium is arranged in the closed box body, a carrier plate is arranged above the liquid level of the liquid medium, and a plurality of cell bottles filled with cell sap are arranged on the carrier plate. According to the utility model, the plurality of cell bottles are placed in the closed box body, and synchronous pressurization of the plurality of cell bottles is completed by utilizing a mode of directly pressurizing the interior of the closed box body and a principle of transferring pressure to the solid bottle bodies, so that the number of pressurized cell bottles is increased, and meanwhile, the operation of changing the liquid of the cell bottles is simplified, and the liquid can be changed by opening the cover body.

Description

Technical Field

[0001] The utility model relates to the technical field of cell pressurization, in particular to a batch pressurization device for bottled cells. Background Art

[0002] Cell pressure experiments are used to study changes in cells under conditions that simulate physiological pressure. The internal pressures of different parts of the human body are different. Biological studies of cells under different pressures are of great significance to the study of brain tumors, glaucoma, etc.

[0003] At present, the existing cell pressurizing devices are still mainly used for directly pressurizing single cell culture dishes / bottles, the number of pressurized cells is small, and when changing the fluid, the pressurizing device needs to be removed from the cell culture dish / bottle, which makes the fluid change troublesome and long-term culture cumbersome. Utility Model Content

[0004] The utility model aims to provide a batch pressurizing device for bottled cells, so as to solve the technical problems in the prior art that the liquid replacement is troublesome and the pressurizing quantity is small due to the single pressurizing cell culture dish / bottle.

[0005] In order to solve the above technical problems, the utility model specifically provides the following technical solutions:

[0006] A batch pressurizing device for bottled cells comprises a closed box body with a cover body, wherein an air inlet port and a pressure measuring port are provided on the cover body, and the air inlet port and the side pressure port are connected to an external pressure control device and a pressure measuring device through a sealing joint.

[0007] A liquid medium is arranged in the closed box body, a carrier plate is arranged above the liquid surface of the liquid medium, and a plurality of cell bottles filled with cell fluid are arranged on the carrier plate.

[0008] As a preferred solution of the utility model, a plurality of partitions are arranged below the carrier plate, each of the partitions is abutted against the inner wall of the closed box body through a seal, the partitions, the closed box body and the carrier plate together enclose a liquid storage cavity, different liquid media are arranged in the plurality of liquid storage cavities, and a liquid inlet connected to the liquid storage cavity is opened on the side wall of the closed box body, and a liquid pressure indicating tube is detachably installed on the liquid inlet.

[0009] As a preferred solution of the utility model, an isolation component is arranged above the carrier plate, a cell bottle is arranged in each of the isolation components, and the cell bottle in each of the isolation components is aligned with the liquid storage cavity.

[0010] As a preferred solution of the utility model, the isolation assembly includes an isolation plate body, and the isolation plate bodies are symmetrically arranged on the carrier plate in a group of two, and an isolation cavity is formed between the two isolation plate bodies, and the cell bottle is arranged in the isolation cavity;

[0011] The isolation plate body comprises a first limiting portion and a second limiting portion, wherein the second limiting portion is arranged on the carrier plate and connected to the bottom end of the first limiting portion;

[0012] The distance between the two first limiting parts on the isolation cavity is greater than or equal to the width of the cell bottle, the distance between the two second limiting parts is greater than the distance between the two first limiting parts, and the vertical height between the second limiting part and the carrier is greater than the thickness of the cell bottle.

[0013] As a preferred solution of the present invention, the length of the isolation plate body is smaller than the length of the cell bottle.

[0014] Compared with the prior art, the utility model has the following beneficial effects:

[0015] The utility model places a plurality of cell bottles in a closed box body, utilizes the method of directly pressurizing the inside of the closed box body and the principle of pressure transmission of a solid bottle body, thereby achieving synchronous pressurization of the plurality of cell bottles, thereby increasing the number of pressurized cell bottles and simplifying the operation of changing the liquid in the cell bottles, and the liquid can be changed by opening the cover body.

[0016] In addition, by providing baffles and isolation components, the effects of different liquid media on cell fluid during synchronous pressurization can be further studied, thus expanding the research function of the batch pressurization device. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] In order to more clearly illustrate the implementation of the utility model or the technical solution in the prior art, the following is a brief introduction to the drawings required for the implementation or the prior art description. Obviously, the drawings in the following description are only exemplary, and for ordinary technicians in this field, other implementation drawings can be derived from the provided drawings without creative work.

[0018] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0019] Figure 2 It is a side view of the utility model.

[0020] The numbers in the figure represent the following:

[0021] 1. Cover body; 2. Enclosed box body; 3. Air inlet; 4. Pressure measuring pipe port; 5. Liquid medium; 6. Carrier plate; 7. Cell bottle; 8. Partition plate; 9. Liquid storage chamber; 10. Isolation assembly; 11. Isolation plate body; 12. Isolation chamber; 13. First limiting part; 14. Second limiting part; 15. Liquid inlet; 16. Liquid pressure indicating tube. DETAILED DESCRIPTION

[0022] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0023] like Figure 1 As shown, the utility model provides a closed box body 2 including a cover body 1, on which an air inlet port 3 and a pressure measuring port 4 are provided, and both the air inlet port 3 and the side pressure port are connected to an external pressure control device and a pressure measuring device through a sealing joint.

[0024] A liquid medium 5 is arranged in the closed box body 2 , a carrier plate 62 is arranged above the liquid surface of the liquid medium 5 , and a plurality of cell bottles 7 filled with cell fluid are arranged on the carrier plate 6 .

[0025] The external pressure control device is used to pressurize the interior of the closed box body 2 and control the degree of pressure input. The pressure measuring device is used to monitor the pressure value inside the closed shell in real time, and a display device can be further connected to the closed box body 2 to further display the internal pressure value.

[0026] Among them, due to its own characteristics, the pressure of the liquid medium 5 can be transmitted to other positions in an instant, which makes the pressure input from a single point into the closed box body 2, and can be instantly diffused to other positions of the closed box body 2 through the liquid medium 5, so that the pressure distribution inside is uniform. When studying multiple cell bottles 7 pressurizing the same liquid medium 5, pure water can be used for the liquid medium 5. The internal humidity can also be maintained during the pressurization process, which is beneficial to cell culture.

[0027] Further, for different liquid media 5, their density, compressibility, volatility and other characteristics are different, so a plurality of partitions 8 are arranged below the carrier plate 6, each partition 8 is abutted against the inner wall of the closed box body 2 through a seal, and the partition 8, the closed box body 2 and the carrier plate 6 are enclosed together to form a liquid storage cavity 9, and different liquid media 5 are arranged in the plurality of liquid storage cavities 9, and a liquid inlet connected to the liquid storage cavity 9 is opened on the side wall of the closed box body 2, and a liquid pressure indicating tube is detachably installed on the liquid inlet. After the liquid pressure indicating tube 16 is arranged, the pressure level in each liquid storage cavity 9 can be reflected in real time through the liquid level height of different liquid media 5 in the liquid storage cavity 9 rising in the liquid pressure indicating tube 16. The pressure monitoring and regulation in the liquid storage cavity 9 are made more accurate. Among them, the liquid pressure indicating tube 16 can be a hollow circular tube with a diameter of 1 mm, and connected to the liquid inlet by a one-way valve. And the connection method can be a detachable connection, so as to facilitate the filling / discharging of the liquid storage cavity 9 from the liquid inlet 15.

[0028] By setting different liquid media 5 to simulate different physiological environments of cells in the human body, multiple types can be tested and studied when pressurized simultaneously, thereby expanding the function of the device and enhancing the practicality of the device. Among them, the carrier plate 6 is set as a perforated plate, which can reduce the loss of a certain pressure during transmission.

[0029] In addition, in order to strengthen the placement specifications of the cell bottle 7 on the carrier 6, reduce the contact between multiple cell bottles 7 and adjacent cell bottles 7, and make the cell bottles 7 correspond to different media in their respective liquid storage chambers 9, an isolation assembly 10 is further arranged above the carrier 6, and a cell bottle 7 is arranged in each isolation assembly 10, and the cell bottle 7 in each isolation assembly 10 is aligned with the liquid storage chamber 9.

[0030] Furthermore, the isolation assembly 10 includes an isolation plate body 11, and the isolation plate bodies 11 are symmetrically arranged on the carrier plate 6 in a group of two, and an isolation cavity 12 is formed between the two isolation plate bodies 11, and the cell bottle 7 is arranged in the isolation cavity 12;

[0031] The isolation plate body 11 includes a first limiting portion 13 and a second limiting portion 14, wherein the second limiting portion 14 is disposed on the carrier plate 6 and connected to the bottom end of the first limiting portion 13;

[0032] The distance between the two first limiting portions 13 on the isolation chamber 12 is greater than or equal to the width of the cell bottle 7 , the distance between the two second limiting portions 14 is greater than the distance between the two first limiting portions 13 , and the vertical height between the second limiting portion 14 and the carrier plate 6 is greater than the thickness of the cell bottle 7 .

[0033] The isolation assembly 10 can prevent the operator from placing multiple cell bottles 7 improperly, causing problems such as bottle contact and bottle stacking. Further limiting the isolation assembly 10 can prevent the operator from placing the cell bottle 7 into the isolation assembly 10 without contacting the isolation plate 11, thereby reducing the contact points and areas between the cell bottle 7 and the solid, and reducing the influence of external components on the cell bottle 7 when pressure is applied to the cell bottle 7.

[0034] In actual experiments, the cell bottle 7 is mostly a flat loading bottle with a bottle mouth, which is similar to a small wine bottle. It is placed on the carrier 6 in a flat posture with the bottle mouth facing forward to spread the internal cell fluid as flat as possible in the bottle body, and then pressurized.

[0035] Therefore, the setting of the isolation chamber 12 in the present device is also adapted to this type of bottle body, and the distance between the two first limiting portions 13 on the isolation chamber 12 is greater than or equal to the width of the cell bottle 7, so that the cell bottle 7 can be placed flat between the two first limiting portions 13 until it is placed on the carrier 6. Furthermore, the distance between the two second limiting portions 14 is greater than the distance between the two first limiting portions 13, which makes it possible for the cell bottle 7 placed between the two first limiting portions 13 to only go vertically downward and cannot contact the second limiting portions 14 on both sides, thereby avoiding contact between the side of the bottle body and the isolation plate body 11.

[0036] In addition, the vertical height between the second limiting portion 14 and the carrier plate 6 is greater than the thickness of the cell bottle 7, so that the upper surface of the bottle placed on the carrier plate 6 will not contact the bottom of the first limiting portion 13 or the top of the second limiting portion 14, further avoiding the contact between the bottle and the isolation plate body 11. Therefore, when the bottle is naturally placed on the carrier plate 6 from between the two first limiting portions 13, the contact between adjacent bottles and the contact between the bottle and the isolation plate body 11 can be avoided.

[0037] Furthermore, the length of the isolation plate body 11 is set to be smaller than the length of the cell bottle 7. Then it will be more convenient to pick up the cell bottle 7. Since the cell bottle 7 is placed flat between the two first limiting parts 13, and if the distance between the first limiting parts 13 is set to be relatively small in order to save the internal space of the closed box body 2, it will be inconvenient to pinch the two sides of the bottle body when it is placed flat. The operator will instead pick it up by the bottleneck. Furthermore, if the longitudinal length of the entire isolation plate body 11 is greater than the length of the cell bottle 7, it will still be inconvenient to pick it up even if you pinch the bottleneck to pick it up. Therefore, the length of the isolation plate body 11 is set to be smaller than the length of the cell bottle 7. This setting will not only have no effect on the pressurization effect, but also improve the convenience of using the device.

[0038] Furthermore, if a vertical bottle body is required in the laboratory, that is, the bottle body bottom is placed vertically on the carrier plate 6 for pressurization operation, the device can limit the position of the bottle body through the following two embodiments.

[0039] First, a vertical groove 15 is provided on the opposite sides of the two first limiting parts 13 on the isolation chamber 12. The width of the groove 15 is greater than the thickness of the cell bottle 7 so as to vertically place the cell bottle 7 into the groove 15, and the distance between the groove 15 and the carrier plate 6 is greater than the height of the cell bottle 7.

[0040] After the cell bottle 7 is placed into the isolation chamber 12 through the groove 7, since the distance between the groove 15 and the carrier plate 6 is greater than the height of the cell bottle 7, when the cell bottle 7 is placed on the carrier plate 6, it will not contact the isolation plate body 11, thereby improving the pressurization effect of a single bottle.

[0041] However, the above arrangement also has some disadvantages. If the distance between the groove 7 and the carrier plate 6 is greater than the height of the cell bottle 7, a larger space will be occupied, so that the closed box body 21 must have a larger volume. At the same time, the bottle body that is not in contact with the isolation plate body 11 is not stable enough when placed vertically, and there is a risk of shaking and tipping.

[0042] Therefore, the vertical bottle body can also be loaded through the following second setting.

[0043] A vertical groove 15 is provided on the side opposite to the two first limiting parts 13 on the isolation chamber 12. The width of the groove 15 is greater than the thickness of the cell bottle 7 so as to vertically place the cell bottle 7 into the groove 15. The distance between the groove 15 and the carrier plate 6 is less than the height of the body of the cell bottle 7. A limiting clamping point 16 is provided on the inner wall of the groove 15, and the limiting clamping point 16 contacts the body of the cell bottle 7.

[0044] The bottle body is limited by setting a limit clamping point 16 in the groove 15 and utilizing the limit clamping point 16 to contact with the bottle body.

[0045] Furthermore, the carrier plate 62 and each isolation assembly 8 are provided with a plurality of perforations, which enable the internal pressure to act on the bottle body through the perforations, thereby achieving a better pressurization effect on the cells.

[0046] The above embodiments are only exemplary embodiments of the present application and are not intended to limit the present application. The protection scope of the present application is defined by the claims. Those skilled in the art may make various modifications or equivalent substitutions to the present application within the essence and protection scope of the present application, and such modifications or equivalent substitutions shall also be deemed to fall within the protection scope of the present application.

Claims

1. A batch pressurizing device for bottled cells, characterized in that: It comprises a closed box body (2) having a cover body (1), an air inlet pipe port (3) and a pressure measuring pipe port (4) are provided on the cover body (1), and the air inlet pipe port (3) and the pressure measuring pipe port (4) are both connected to an external pressure control device and a pressure measuring device via a sealing joint; A liquid medium (5) is arranged in the closed box body (2), a carrier plate (6) is arranged above the liquid surface of the liquid medium (5), and a plurality of cell bottles (7) filled with cell fluid are arranged on the carrier plate (6).

2. The batch pressurizing device for bottled cells according to claim 1, characterized in that: A plurality of partitions (8) are arranged below the carrier plate (6), each of the partitions (8) abuts against the inner wall of the closed box body (2) through a sealing member, and the partitions (8), the closed box body (2) and the carrier plate (6) together enclose a liquid storage cavity (9), and different liquid media (5) are arranged in the plurality of liquid storage cavities (9), and a liquid inlet (15) connected to the liquid storage cavity (9) is opened on the side wall of the closed box body (2), and a liquid pressure indicating tube (16) is detachably mounted on the liquid inlet (15).

3. The batch pressurizing device for bottled cells according to claim 2, characterized in that: An isolation component (10) is arranged above the carrier plate (6), a cell bottle (7) is arranged in each of the isolation components (10), and the cell bottle (7) in each of the isolation components (10) is aligned with the liquid storage chamber (9).

4. The batch pressurizing device for bottled cells according to claim 3, characterized in that: The isolation assembly (10) comprises an isolation plate body (11), wherein two isolation plate bodies (11) are symmetrically arranged on the carrier plate (6), an isolation cavity (12) is formed between the two isolation plate bodies (11), and the cell bottle (7) is arranged in the isolation cavity (12); The isolation plate body (11) comprises a first limiting portion (13) and a second limiting portion (14), wherein the second limiting portion (14) is arranged on the carrier plate (6) and connected to the bottom end of the first limiting portion (13); The distance between the two first limiting portions (13) on the isolation chamber (12) is greater than or equal to the width of the cell bottle (7), the distance between the two second limiting portions (14) is greater than the distance between the two first limiting portions (13), and the vertical height between the second limiting portion (14) and the carrier plate (6) is greater than the thickness of the cell bottle (7).

5. The batch pressurizing device for bottled cells according to claim 4, characterized in that: The length of the isolation plate body (11) is smaller than the length of the cell bottle (7).

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