Layered tissue cell culture container
By designing a layered tissue cell culture container, using the gap between the lower layer and the upper dish to carry the tissue block, and replace the medium through the hole to resolve the traditional cultural dish. The problem has achieved more stable and efficient cultivation of tissue cells.
Patent Information
- Application Number
- CN202422360156.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-09-27
- Publication Date
- 2025-01-28
- Estimated Expiration
- 2034-09-27
AI Technical Summary
When a traditional Petri dish or culture flask is replaced, the flow of the culture medium will affect the adhesion of tissue blocks, which in turn affects the primary culture of tissue cells.
A layered tissue cell culture container is designed, including the lower layer and the upper dish. A gap is formed between the two to carry the tissue block, and the bottom edge of the upper dish is opened with a hole It does not affect the sticker of the tissue block.
Through the layered design, the tissue block will not be affected by the flow when replacing the medium, which reduces the impact of the original culture of tissue cells, and the operation of the replacement of the medium becomes more convenient.
Smart Images
Figure CN222411630U_ABST
Abstract
Description
Technical Field
[0001] The present application relates to the field of biomedicine technology, and in particular to a layered tissue cell culture container. Background Art
[0002] As the most basic and classic laboratory equipment for culturing microorganisms, cells or tissue blocks, culture dishes play an important role in studying cells in the field of biomedicine. At present, the in vitro culture of cells and tissues has become an indispensable part of life research and practice. Among them, tissue culture is a technology that sterilely removes tissue blocks and cell groups from individuals of multicellular organisms and keeps them alive under appropriate conditions. It is now not only widely used in the extraction of primary cells, cell reproduction, differentiation, and cancer research, but also uses tissue cell culture to study drug effects. Under existing conditions, the main methods for culturing tissue cells are traditional culture dishes and culture bottles.
[0003] When culturing tissue cells in the above-mentioned traditional culture dishes or culture bottles for primary cell culture, tissue blocks are generally placed at the bottom of the culture dishes or culture bottles. When replacing the culture medium, the flow of the culture medium will affect the attachment of the tissue blocks and thus greatly affect the primary culture of the tissue cells. Utility Model Content
[0004] In view of this, the embodiment of the present application provides a layered tissue cell culture container to solve the problems caused by the operation of replacing the culture medium in the culture dish in the background technology.
[0005] The present application embodiment provides a layered tissue cell culture container, comprising:
[0006] A lower dish body, wherein the lower dish body is hollow inside and has an opening at the upper end, and the upper surface of the bottom of the lower dish body is used to carry tissue blocks or culture medium;
[0007] An upper dish body, wherein the interior of the upper dish body is hollow and the upper end is provided with an opening, and the bottom upper surface of the upper dish body is used to hold the culture medium; at least one through hole is provided at the bottom edge of the upper dish body;
[0008] The upper end of the upper dish body has a supporting edge protruding outward, the lower end of the upper dish body is embedded in the lower dish body, and the upper dish body is supported at the upper end opening of the lower dish body by the supporting edge, and a gap for accommodating the tissue block is formed between the bottom lower surface of the upper dish body and the bottom upper surface of the lower dish body.
[0009] Optionally, in the layered tissue cell culture container of the present invention, three through holes are provided, with the center of the upper dish body as the rotation center, and one through hole is provided every 90 degrees.
[0010] Optionally, in the layered tissue cell culture container of the present invention, the upper dish body is in the shape of a truncated cone with a lower outer diameter smaller than an upper outer diameter, and the upper outer diameter of the upper dish body is equal to the upper inner diameter of the lower dish body.
[0011] Optionally, in the layered tissue cell culture container of the present invention, the width of the gap is 1 mm to 3 mm.
[0012] Optionally, in the layered tissue cell culture container of the present invention, the diameter of the through hole is 4 mm to 6 mm.
[0013] Optionally, the layered tissue cell culture container of the utility model further comprises a culture dish cover, wherein the culture dish cover covers the upper end of the upper dish body.
[0014] Optionally, in the layered tissue cell culture container of the present invention, the side wall of the lower dish body protrudes outward with an outer edge, and the outer edge is used to support the culture dish cover.
[0015] Optionally, in the layered tissue cell culture container of the present invention, the outer edge is in a circular ring shape.
[0016] Optionally, in the layered tissue cell culture container of the present invention, the edge of the outer edge is wavy.
[0017] Optionally, in the layered tissue cell culture container of the present invention, the edge of the outer rim has a frosted surface.
[0018] The layered tissue cell culture container provided in the embodiment of the present application is matched with the lower dish body and the upper dish body. When the culture medium is replaced, the tissue block is attached between the lower dish body and the upper dish body, and the flow of the culture medium will not affect the tissue block, thereby reducing the impact on the primary culture of the tissue cells, and the culture medium can be replaced very conveniently.
[0019] Additional aspects and advantages of the present application will be given in part in the description below, and in part will become apparent from the description below, or will be learned through the practice of the present application. BRIEF DESCRIPTION OF THE DRAWINGS
[0020] The drawings described herein are used to provide a further understanding of the present application and constitute a part of the present application. The illustrative embodiments of the present application and their descriptions are used to explain the present application and do not constitute an improper limitation on the present application. In the drawings:
[0021] Figure 1 An exploded schematic diagram of a layered tissue cell culture container structure provided in an embodiment of the present application;
[0022] Figure 2 A schematic diagram of the assembly of a layered tissue cell culture container structure provided in an embodiment of the present application;
[0023] Figure 3 This is a schematic diagram of the cross-sectional structure of a layered tissue cell culture container provided in an embodiment of the present application.
[0024] The reference numerals in the figure are:
[0025] 1. Petri dish cover;
[0026] 2. Upper dish body;
[0027] 3. Through hole;
[0028] 4. Lower dish body;
[0029] 5. Outer edge;
[0030] 6. Tissue block;
[0031] 21. Support edge. DETAILED DESCRIPTION
[0032] The exemplary embodiments disclosed in the present application will be described in more detail below with reference to the accompanying drawings. Although the exemplary embodiments of the present application are shown in the accompanying drawings, it should be understood that the present application can be implemented in various forms and should not be limited by the specific embodiments described herein. On the contrary, these embodiments are provided to enable a more thorough understanding of the present application and to fully convey the scope disclosed in the present application to those skilled in the art.
[0033] In the following description, a large number of specific details are given to provide a more thorough understanding of the present application. However, it is obvious to those skilled in the art that the present application can be implemented without one or more of these details. In other examples, in order to avoid confusion with the present application, some technical features known in the art are not described; that is, all features of the actual embodiments are not described here, and well-known functions and structures are not described in detail.
[0034] Spatial relationship terms such as "under", "below", "below", "under", "above", "above", etc., may be used here for convenience of description to describe the relationship between an element or feature shown in the figure and other elements or features. It should be understood that in addition to the orientation shown in the figure, the spatial relationship terms are intended to include different orientations of the device in use and operation. For example, if the device in the accompanying drawings is turned over, then the elements or features described as "under other elements" or "under it" or "under it" will be oriented as "on" other elements or features. Therefore, the exemplary terms "under" and "under" may include both upper and lower orientations. The device can be oriented otherwise (rotated 90 degrees or other orientations) and the spatial descriptors used herein are interpreted accordingly.
[0035] The purpose of the terms used herein is only to describe specific embodiments and is not intended to be limiting of the present application. When used herein, the singular forms "one", "an" and "said / the" are also intended to include plural forms, unless the context clearly indicates otherwise. It should also be understood that the terms "consisting of" and / or "comprising", when used in this specification, determine the presence of the features, integers, steps, operations, elements and / or parts, but do not exclude the presence or addition of one or more other features, integers, steps, operations, elements, parts and / or groups. When used herein, the term "and / or" includes any and all combinations of the relevant listed items.
[0036] In order to thoroughly understand the present application, detailed steps and detailed structures will be presented in the following description to illustrate the technical solution of the present application. The preferred embodiments of the present application are described in detail below, but in addition to these detailed descriptions, the present application may also have other implementation methods.
[0037] This embodiment provides a layered tissue cell culture container, such as Figure 1-Figure 3 As shown, including:
[0038] A lower dish body 4, wherein the lower dish body 4 is hollow inside and has an opening at the upper end, and the bottom upper surface of the lower dish body 4 is used to carry tissue blocks 6 or culture medium;
[0039] An upper dish body 2, wherein the upper dish body 2 is hollow inside and has an opening at the upper end, and the upper surface of the bottom of the upper dish body 2 is used to hold culture medium; at least one through hole 3 is formed at the bottom edge of the upper dish body 2;
[0040] The upper end of the upper dish body 2 has a supporting edge 21 protruding outward, the lower end of the upper dish body 2 is embedded in the lower dish body 4, and the upper dish body 2 is supported at the upper end opening of the lower dish body 4 by the supporting edge 21, and a gap for accommodating the tissue block 6 is formed between the bottom lower surface of the upper dish body 2 and the bottom upper surface of the lower dish body 4.
[0041] The working principle of the layered tissue cell culture container of this embodiment is:
[0042] The bottom upper surface of the lower dish body 4 can carry tissue blocks 6 or culture medium. When in use, the tissue blocks 6 can be placed on the bottom of the lower dish body 4, and then the upper dish body 2 is installed. Figure 3As shown, the tissue block 6 is sandwiched between the bottom lower surface of the upper dish body 2 and the bottom upper surface of the lower dish body 4. In this state, even if the tissue block 6 is impacted by the flow of the culture medium in the gap, it will not float much. The bottom edge of the upper dish body 2 is provided with at least one through hole 3, and the through hole 3 can allow the culture medium added to the upper dish body 2 to flow into the lower dish body 4. The method of replacing the culture medium is to tilt the upper dish body 2 and the lower dish body 4 at the same time, and let the position where the through hole 3 is located tilt upward, so that the culture medium to be replaced is gathered at a position away from the through hole 3. At this time, a pipette can be used to discard the gathered old culture medium, and the old culture medium in the gap can be left untreated due to its small amount. When adding fresh culture medium, the upper dish body 2 and the lower dish body 4 are laid flat, and fresh culture medium is added to the upper dish body 2, and the fresh culture medium naturally flows into the bottom of the lower dish body 4 through the through hole 3. During the entire operation of replacing the culture medium, on the one hand, since the tissue block 6 is sandwiched between the upper dish body 2 and the lower dish body 4, the surfaces of the upper dish body 2 and the lower dish body 4 have an adsorption force on the tissue block 6, and on the other hand, since the flow amount of the old culture medium in the gap is much smaller than the flow amount in the upper dish body 2, the tissue block 6 is little affected by the flow environment of the culture medium and will not float.
[0043] Optionally, the layered tissue cell culture container of this embodiment, such as Figure 1 As shown, there are three through holes 3, with the center of the upper dish body 2 as the rotation center, and one through hole 3 is set every 90 degrees. Described by the clock positioning method, in this optional solution, through holes 3 are set at the 12 o'clock direction, the 3 o'clock direction and the 6 o'clock direction of the upper dish body 2. Under this structure, when replacing the culture medium, the 3 o'clock position of the upper dish body 2 and the lower dish body 4 can be raised, so that the culture medium is gathered at the 9 o'clock position without the through hole 3, so as to discard the old culture medium; when the upper dish body 2 and the lower dish body 4 are laid flat, the new culture medium injected into the upper dish body 2 can quickly flow into the bottom from the three through holes, which is more efficient than the design of a single through hole.
[0044] Optionally, in the layered tissue cell culture container of this embodiment, the upper dish body 2 is a truncated cone with a lower outer diameter smaller than an upper outer diameter, and the upper outer diameter of the upper dish body 2 is equal to the upper inner diameter of the lower dish body 4. In this optional solution, the truncated cone design of the upper dish body 2 can facilitate the placement of the upper dish body 2 into the lower dish body 4, and can ensure that the center of the upper dish body 2 is aligned with the center of the lower dish body 4, which is convenient for the central placement of the tissue block 6 and is also conducive to the tilting operation when changing the culture medium.
[0045] Optionally, in the layered tissue cell culture container of this embodiment, the width of the gap is 1 mm to 3 mm, preferably 2 mm. With this gap width, the tissue block 6 can be better attached to the surface of the upper dish body 2 and the lower dish body 4.
[0046] Optionally, in the layered tissue cell culture container of this embodiment, the diameter of the through hole 3 is 4 mm to 6 mm, preferably 5 mm. Under this through hole diameter, the through hole 3 is large enough for the culture medium to pass through quickly, and the placement space of the tissue block 6 will not be affected due to the through hole 3 being too large.
[0047] Optionally, the layered tissue cell culture container of this embodiment, such as Figure 1-Figure 3 As shown, the culture dish also includes a culture dish cover 1, which covers the upper end of the upper dish body 2. The culture dish cover 1 can play a role in sealing the upper dish body 2 to prevent the culture medium from splashing out when the culture medium is replaced.
[0048] Optionally, the layered tissue cell culture container of this embodiment, such as Figure 1 As shown, the side wall of the lower dish body 4 protrudes outward with an outer edge 5, and the outer edge 5 is used to support the culture dish cover 1. The setting of the outer edge 5 can facilitate the operator to hold the container, and when holding the outer edge 5, the operator can generally touch the culture dish cover 1 at the same time to prevent the culture dish cover 1 from falling.
[0049] Optionally, the layered tissue cell culture container of this embodiment, such as Figure 1 As shown, the outer edge 5 is in the shape of a circular ring, which can more comprehensively support the culture dish cover 1.
[0050] Optionally, in the layered tissue cell culture container of this embodiment, the edge of the outer edge 5 is wavy. The wavy structure can increase the surface area, so that the operator has a larger contact surface when holding it, and reduce the possibility of slipping.
[0051] Optionally, in the layered tissue cell culture container of this embodiment, the edge of the outer edge 5 has a frosted surface to increase friction, so that the friction is greater when the operator holds it, reducing the possibility of slipping.
[0052] It should be understood that the above embodiments are exemplary and are not intended to include all possible implementations included in the claims. Various modifications and changes may be made on the basis of the above embodiments without departing from the scope of the present disclosure. Similarly, the various technical features of the above embodiments may be arbitrarily combined to form other embodiments of the present application that may not be explicitly described. Therefore, the above embodiments only express several implementations of the present application and do not limit the scope of protection of the patent of this application.
Claims
1. A layered tissue cell culture container, characterized in that: include: A lower dish body (4), the lower dish body (4) is hollow inside and has an opening at the upper end, and the upper surface of the bottom of the lower dish body (4) is used to carry tissue blocks (6) or culture medium; An upper dish body (2), the interior of the upper dish body (2) is hollow and has an opening at the upper end, and the upper surface of the bottom of the upper dish body (2) is used to hold a culture medium; at least one through hole (3) is formed at the bottom edge of the upper dish body (2); The upper end of the upper dish body (2) has a supporting edge (21) protruding outward, the lower end of the upper dish body (2) is embedded in the lower dish body (4), and the upper dish body (2) is supported at the upper end opening of the lower dish body (4) through the supporting edge (21), and a gap for accommodating the tissue block (6) is formed between the bottom lower surface of the upper dish body (2) and the bottom upper surface of the lower dish body (4).
2. The layered tissue cell culture container according to claim 1, characterized in that: The through holes (3) are provided in three numbers, with the center of the upper dish body (2) being the rotation center, and one through hole (3) being provided every 90 degrees.
3. The layered tissue cell culture container according to claim 1, characterized in that: The upper dish body (2) is in the shape of a truncated cone with a lower outer diameter smaller than an upper outer diameter, and the upper outer diameter of the upper dish body (2) is equal to the upper inner diameter of the lower dish body (4).
4. The layered tissue cell culture container according to claim 1, characterized in that: The width of the gap is 1 mm to 3 mm.
5. The layered tissue cell culture container according to any one of claims 1 to 4, characterized in that: The diameter of the through hole (3) is 4 mm to 6 mm.
6. The layered tissue cell culture container according to any one of claims 1 to 4, characterized in that: It also comprises a culture dish cover (1), wherein the culture dish cover (1) covers the upper end of the upper dish body (2).
7. The layered tissue cell culture container according to claim 6, characterized in that: The side wall of the lower dish body (4) protrudes outwards to form an outer edge (5), and the outer edge (5) is used to support the culture dish cover (1).
8. The layered tissue cell culture container according to claim 7, characterized in that: The outer edge (5) is in the shape of a circular ring.
9. The layered tissue cell culture container according to claim 8, characterized in that: The edge of the outer edge (5) is wavy.
10. The layered tissue cell culture container according to claim 8, characterized in that: The edge of the outer edge (5) has a frosted surface.