Portable high-flux fluorescence detection device
By designing a portable high -throughput fluorescent detection device, the combination of rotating detection cards and shading plates solves the problem of not flexible and heavy volume of the existing fluorescent detection device, and achieves the accuracy of high -efficiency and flexible fluorescent detection and detection results.
Patent Information
- Application Number
- CN202420858600.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-04-23
- Publication Date
- 2025-01-28
- Estimated Expiration
- 2034-04-23
AI Technical Summary
The existing fluorescence detection device is not flexible enough when detecting in a light-proof environment, requires multiple samples to be changed and is bulky, making it not suitable for portable detection.
The portable high -throughput fluorescent detection device is designed. The box and detection mechanism are used. The side wall of the box has a opening and shading plate. Merchants and flexible detection are achieved by rotating detection cards and shading plates.
The efficient fluorescence detection in the sealing dark environment is achieved, the detection efficiency is improved, and the design of dust and other impurities can be prevented through the design of the shading plate to ensure the accuracy of the test results.
Smart Images
Figure CN222419979U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of biological sample fluorescence detection, in particular to a portable high-throughput fluorescence detection device. Background Art
[0002] Fluorescence detection is a detection method that uses a fluorescence detection device to measure the content of a certain type of specific substance in a sample. The working principle of the fluorescence detection device is as follows: a fluorescently labeled monoclonal antibody and a capture monoclonal antibody are coated on the test card. When the sample is added to the test card, the fluorescently labeled monoclonal antibody combines with the analyte in the sample to form an antigen-antibody complex; then the probe automatically scans the binding area of the marker and the analyte to obtain an optical signal; then the photoelectric conversion module measures and analyzes the optical signal to quantitatively determine the content of the analyte, and sends the result data to the system to finally complete the sample detection.
[0003] Fluorescence detection is easily affected by environmental interference from external light, which affects the accuracy and sensitivity of the detection results. Most of the existing fluorescence detection devices set up a fixed dark slot inside the device, and place the sample to be tested in the dark slot when measuring fluorescence to perform high-sensitivity detection in a light-proof environment; however, this operation method has the defects of requiring multiple sample changes and time-consuming operation. The light-proof detection area is not flexible enough, and the sample cannot be flexibly replaced without opening the lid of the detection device, thereby reducing the detection efficiency. In addition, due to the principle of fixed light-proof area, most of the existing fluorescence detection devices are bulky and not suitable for portable detection. Utility Model Content
[0004] The utility model aims to provide a portable high-throughput fluorescence detection device, which can accurately and efficiently perform fluorescence detection in a light-proof environment.
[0005] In order to achieve the above-mentioned purpose, the utility model adopts the following technical scheme: a portable high-throughput fluorescence detection device, including a box and a detection mechanism, the detection mechanism is arranged inside the box, the side wall of the box is provided with an opening, and a light shielding plate is slidably connected in the opening; a loading plate is provided in the box, and a detection card is rotatably connected to the loading plate, and a plurality of detection areas are provided on the detection card, and the detection card rotates to drive the plurality of detection areas to change positions in turn under the detection mechanism.
[0006] The beneficial effect of this solution is as follows: by rotating a detection card with several detection areas and connecting it inside a box, the box ensures the shading effect through a shading plate. When the detection card is rotated, different detection areas are rotated in turn to the bottom of the detection mechanism for alternating detection, so that efficient fluorescence detection can be performed in a sealed dark environment.
[0007] At the same time, after the box is completely shielded by the shading plate, the shaft remains in a closed environment under the shielding effect of the shading plate when the test card is not inserted, preventing dust and other impurities from entering the inside of the box and blocking the laser emission port of the detection probe in the detection mechanism, thereby affecting the accuracy of the test results.
[0008] Preferably, a rotating shaft is rotatably connected to the carrier plate, and the detection card is detachably connected to the rotating shaft; a support seat is provided below the carrier plate, a driving member is provided inside the support seat, and the driving member is connected to the bottom of the rotating shaft.
[0009] Preferably, the opening and the shading plate are both in the shape of a frustum, and a sealing adhesive layer is provided on the side wall of the shading plate.
[0010] The beneficial effects of this solution are: 1. The frustum shape can play a positioning role when the shading plate is pushed into the opening, so as to facilitate the operation of pushing the shading plate into the opening for fixed connection;
[0011] 2. The contact area between the frustum-shaped shading plate and the frustum-shaped opening is increased, so that the area of the sealant layer that can be set is larger, and the stability of the connection between the shading plate and the opening after being fitted can be further improved;
[0012] 3. The contact surfaces between the cone-shaped shading plate and the opening are all inclined surfaces, and the side walls of the opening of the inclined surface can be covered and shielded by the shading plate which is also an inclined surface, thereby further improving the shading effect on the interior of the box.
[0013] Preferably, the detection mechanism includes a connecting rod, a detection probe and a battery, the connecting rod is arranged on the inner wall of the box body, the detection probe is connected to the end of the connecting rod, and the probe is connected to a photoelectric conversion module, and the photoelectric conversion module is connected to the battery.
[0014] Preferably, the end of the carrier plate is fixedly connected to the shading plate, and the carrier plate is slidably connected to the surface of the support seat; a roller is provided at the bottom of the driving member, and a sliding groove is provided on the surface of the support seat.
[0015] The beneficial effect of this solution is that when the test card needs to be replaced, pulling out the shading plate will drive the support plate to slide outward, thereby driving the rotating shaft and the test card to move toward the opening. Moving the test card to the opening can make it easier to replace the test card.
[0016] Preferably, a slideway is provided inside the support seat, and a roller at the bottom of the driving member rotates in the slideway. BRIEF DESCRIPTION OF THE DRAWINGS
[0017] Figure 1 It is a three-dimensional diagram of the detection device in Example 1 of the utility model;
[0018] Figure 2This is a schematic diagram of the internal structure of the detection device in Example 1 of the utility model;
[0019] Figure 3 This is a schematic diagram of the internal structure of the detection device in Example 2 of the utility model. DETAILED DESCRIPTION
[0020] The following is further described in detail through specific implementation methods:
[0021] The figure marks in the drawings of the specification include: box body 1, opening 11, top cover 12, shading plate 2, pull ring 21, loading plate 3, rotating shaft 31, supporting seat 32, slide groove 321, limiting groove 322, detection card 4, detection area 41, detection probe 5, connecting rod 51.
[0022] Example 1
[0023] Embodiment 1 is basically as attached Figure 1-2 As shown, Figure 1 A portable high-throughput fluorescence detection device is shown, comprising a box 1 and a detection mechanism installed inside the box 1, and the detection mechanism can realize high-throughput fluorescence detection of the sample to be tested. The top cover of the box 1 is connected to a top cover 12, and the top cover 12 is bonded and fixed with a black sealant layer at the places where the top cover 12 contacts the box 1 on all sides. The black sealant layer further ensures the light-shielding effect inside the box 1. The top cover 12 is provided to facilitate the repair and replacement of the inside of the box 1 including the detection mechanism in the future. The box 1 is equipped with a strap, and the portable operation of the device is realized by the strap.
[0024] like Figure 2As shown, an opening 11 is provided on the side wall of the box body 1, and a shading plate 2 is embedded in the opening 11. A pull ring 21 is hinged on the outer side of the shading plate 2. The pull ring 21 facilitates the push and pull operation of the shading plate 2. The shading plate 2 can be slid and embedded in the opening 11 through the pull ring 21 to completely block the light inside the box body 1, so as to ensure the accuracy of the fluorescence detection result; at the same time, a layer of black sealant layer is also provided at the position where the shading plate 2 contacts the opening 11. The additional sealant layer can not only further ensure the shading effect, but also improve the stability of the connection between the shading plate 2 and the opening 11, thereby ensuring the light-shielding effect inside the box body 1 during the detection process. In addition, in the present embodiment, the opening 11 and the sunshade 2 are both in the shape of a cone, and the cone shape can play a positioning role when the sunshade 2 is pushed into the opening 11, so as to facilitate the operation of pushing the sunshade 2 into the opening 11 for fixed connection; at the same time, the contact area between the cone-shaped sunshade 2 and the cone-shaped opening 11 is increased, and thus the area of the sealant layer that can be set is larger, and the stability of the connection between the sunshade 2 and the opening 11 after engagement can be further improved; and, the contact surfaces between the cone-shaped sunshade 2 and the opening 11 are both inclined surfaces, and the side walls of the opening 11 of the inclined surfaces can be covered and shielded by the sunshade 2 which is also an inclined surface, thereby further improving the shading effect on the interior of the box body 1.
[0025] like Figure 2 As shown, a square support seat 32 is bonded and fixed to the bottom surface of the box 1, and the support seat 32 is hollow. A carrier plate 3 is fixedly connected to the top of the support seat 32, and the carrier plate 3 is also square; a detection card 4 is rotatably connected to the carrier plate 3, and the rotation connection method of the detection card 4 is specifically as follows: a through hole is opened at the top of the carrier plate 3, and a through hole is also opened at the top of the support seat 32, and the upper and lower through holes are coaxial, and a rotating shaft 31 is detachably connected below the detection card 4, and the diameter of the rotating shaft 31 is smaller than the diameter of the two through holes, and the top of the rotating shaft 31 passes through the two through holes and is located above the carrier plate 3; at the same time, a driving member is arranged inside the support seat 32, and in this embodiment, the driving member is a motor, and the bottom of the rotating shaft 31 is connected to the output shaft of the motor through a bearing, and starting the motor can drive the rotating shaft 31 to rotate, thereby driving the rotation of the detection card 4. The motor is connected to a power supply and a switch, and the power supply and the switch are both arranged on the outer wall of the box body 1, so as to continuously ensure the light-shielding property inside the box body 1 during the detection process and ensure the accuracy of the detection result.
[0026] The detection card 4 is a high-throughput fluorescence detection card 4, and a plurality of detection areas 41 are provided on the detection card 4. The rotation of the detection card 4 can drive the plurality of detection areas 41 to sequentially change positions under the detection mechanism. In this embodiment, the detection card 4 is disc-shaped, and a threaded hole is provided at the center of the bottom of the detection card 4, and an external thread is provided on the top of the rotating shaft 31, thereby realizing a detachable connection between the detection card 4 and the rotating shaft 31, so as to facilitate the disassembly of the detection card 4 and the rotating shaft 31 and the replacement of the sample to be tested; after the connection, the direction of the thread is opposite to the rotation direction of the rotating shaft 31, so as to avoid rotation between the detection card 4 and the rotating shaft 31 during the rotation process, thereby ensuring the stability of the connection. Figure 2 As shown, the detection card 4 is provided with four sector-shaped detection areas 41, and different samples to be tested can be placed inside each detection area 41. When the motor is started and the detection card 4 is driven to rotate through the rotating shaft 31, the different detection areas 41 on the detection card 4 are rotated to the bottom of the detection mechanism in turn for fluorescence detection, so that multiple samples can be alternately replaced and tested without taking out the detection card 4, thereby improving the detection efficiency.
[0027] The detection mechanism includes a connecting rod 51, a detection probe 5 and a battery. The connecting rod 51 is fixed to the inner wall of the box body 1 by screws, and the detection probe 5 is connected to the end of the connecting rod 51. At the same time, the probe is also connected to a photoelectric conversion module, which is connected to the battery to perform photoelectric conversion and realize fluorescence detection. Fluorescence detection is a prior art and will not be described in detail in this embodiment.
[0028] The specific implementation process is as follows: first, different samples to be tested are placed in different detection areas 41 on the detection card 4, then the shading plate 2 is removed, and the detection card 4 is installed on the rotating shaft 31 above the carrier plate 3, and then the shading plate 2 is embedded in the opening 11 to seal and shield the detection environment inside the box 1.
[0029] The motor is started by switching on the box 1, and the motor drives the shaft 31 to rotate slowly, so that different detection areas 41 rotate in turn to the bottom of the detection probe 5 in the detection mechanism for fluorescence detection, and the photoelectric conversion module simultaneously uploads the detection results to the system in turn.
[0030] When the test is finished, the motor is turned off, the test card 4 is taken out, and the next test card 4 with multiple samples to be tested is placed in the box 1 to continue the fluorescence test.
[0031] In this solution, the high-throughput fluorescence detection card 4 is detachably mounted on the rotating shaft 31. By rotating the detection card 4 according to the detection requirements, the detection of the detection area 41 can be realized, which greatly improves the detection efficiency; and the high-throughput fluorescence detection card 4 can be repeatedly disassembled and assembled to realize the rapid fluorescence detection of multiple samples. After the box 1 is completely shielded by the shading plate 2, under the shielding effect of the shading plate 2, the rotating shaft 31 is still in a closed environment when the detection card 4 is not inserted, preventing dust and other impurities from entering the box 1 and blocking the laser emission port of the detection probe 5 in the detection mechanism, thereby affecting the accuracy of the detection result.
[0032] Example 2
[0033] like Figure 3 A portable high-throughput fluorescence detection device is shown, which is different from Example 1 in that: the end of the carrier plate 3 is bonded and fixed to the inner wall of the shading plate 2, and the carrier plate 3 is slidably connected to the surface of the support seat 32, a roller is provided at the bottom of the driving member, and a slide groove 321 is provided on the surface of the support seat 32.
[0034] Specifically, a long strip through slot is provided on the surface of the support seat 32, and the rotating shaft 31 passes through the through slot and then through the through hole on the loading board 3 to be located above the loading board 3; at the same time, an inverted T-shaped limiting slot 322 is provided on both sides of the through slot, and an inverted T-shaped slider is fixed on both sides of the bottom of the loading board 3. In this embodiment, the bottom of the motor is slidably connected to the inside of the support seat 32 through a rotatably connected roller, and a long strip slideway is provided at the bottom of the support seat 32, and the roller of the motor is rotatably connected in the slideway to limit the sliding of the motor, thereby ensuring the stability of the motor when sliding.
[0035] like Figure 2 As shown, when the detection card 4 needs to be replaced, pulling out the shading plate 2 will drive the support plate to slide outward, thereby driving the rotating shaft 31 and the detection card 4 to move toward the opening 11. Moving the detection card 4 to the opening 11 can make it easier to replace the detection card 4.
[0036] The above is only an embodiment of the utility model, and the common knowledge such as the known specific technical solutions and / or characteristics in the solution is not described in detail here. It should be pointed out that for those skilled in the art, several deformations and improvements can be made without departing from the technical solution of the utility model, which should also be regarded as the protection scope of the utility model, and these will not affect the effect of the implementation of the invention and the practicality of the patent. The scope of protection required by this application shall be based on the content of its claims, and the specific implementation methods and other records in the specification can be used to interpret the content of the claims.
Claims
1. A portable high-throughput fluorescence detection device, characterized in that: It includes a box body and a detection mechanism, which is arranged inside the box body. An opening is arranged on the side wall of the box body, and a light shielding plate is slidably connected in the opening. A loading plate is arranged in the box body, and a detection card is rotatably connected to the loading plate. The detection card is provided with a plurality of detection areas, and the detection card rotates to drive the plurality of detection areas to change positions in sequence below the detection mechanism.
2. A portable high-throughput fluorescence detection device according to claim 1, characterized in that: A rotating shaft is rotatably connected to the carrier plate, and the detection card is detachably connected to the rotating shaft; a support seat is provided below the carrier plate, a driving member is provided inside the support seat, and the driving member is connected to the bottom of the rotating shaft.
3. A portable high-throughput fluorescence detection device according to claim 2, characterized in that: The opening and the shading plate are both in the shape of a frustum, and a sealing adhesive layer is arranged on the side wall of the shading plate.
4. A portable high-throughput fluorescence detection device according to claim 3, characterized in that: The detection mechanism includes a connecting rod, a detection probe and a battery. The connecting rod is arranged on the inner wall of the box body. The detection probe is connected to the end of the connecting rod. The probe is connected to a photoelectric conversion module, and the photoelectric conversion module is connected to the battery.
5. A portable high-throughput fluorescence detection device according to claim 4, characterized in that: The end of the loading plate is fixedly connected to the shading plate, and the loading plate is slidably connected to the surface of the supporting seat; a roller is arranged at the bottom of the driving member, and a sliding groove is arranged on the surface of the supporting seat.
6. A portable high-throughput fluorescence detection device according to claim 5, characterized in that: A slideway is arranged inside the support seat, and a roller at the bottom of the driving member rotates in the slideway.