Magnetic bead splash-proof device for miniature centrifugal tube

Abstract

The utility model relates to a miniature centrifuge tube magnetic bead splash-proof device which comprises a main body frame, a fixing frame and at least two stabilizing plates, and each stabilizing plate comprises a first part and a second part; the first part is fixed to one side of the main body frame, and the second part is used for abutting against the edges of the two sides of an objective table of the horizontal shaking table. According to the magnetic bead splash-proof device for the miniature centrifugal tube, the miniature centrifugal tube is inserted through the fixing frame, the magnetic beads in the miniature centrifugal tube are attracted through the magnets on the main body frame, and the main body frame is kept stable on the horizontal shaking table by abutting against the objective table of the horizontal shaking table through the stabilizing plate. By means of the arrangement, along with starting of the horizontal shaking table, magnetic bead suspension remaining at the tube opening of the miniature centrifugal tube can be attracted by the magnet on the main body frame, and the situation that the experiment is affected due to the fact that the magnetic bead suspension is splashed out when the miniature centrifugal tube is uncovered is avoided.

Description

Technical Field

[0001] The present application relates to the field of biomedicine technology, and in particular to a micro centrifuge tube magnetic bead splash prevention device. Background Art

[0002] EP tube is a microcentrifuge tube, commonly used in molecular biology experiments. When using the magnetic bead method to extract nucleic acids or extracellular vesicles, the microcentrifuge tube needs to be placed on a magnetic rack to adsorb the magnetic beads. Usually, during the extraction process, vortexing and mixing are required for incubation or washing. After vortexing and mixing, the magnetic beads need to be adsorbed on the magnetic rack. After the magnetic beads are completely adsorbed on the wall of the microcentrifuge tube, the microcentrifuge tube cap is opened to aspirate and discard the liquid in the tube.

[0003] During the extraction process, part of the magnetic bead suspension will adhere to the inner mouth of the microcentrifuge tube after vortexing and mixing. This will cause the magnetic bead suspension at the inner mouth of the tube to fail to adsorb on the tube wall when the microcentrifuge tube is subsequently transferred to the magnetic rack for adsorption. When the microcentrifuge tube cap is opened to aspirate and discard the liquid, the magnetic bead suspension at the inner mouth of the tube will splash out, resulting in sample loss and contamination, affecting the experiment. Utility Model Content

[0004] In view of this, the embodiment of the present application provides a microcentrifuge tube magnetic bead splash-proof device to solve the problem of easy splashing of magnetic bead suspension at the mouth of the microcentrifuge tube existing in the background technology.

[0005] The present application embodiment provides a micro centrifuge tube magnetic bead splash prevention device, comprising:

[0006] A main frame, wherein the main frame has rows of magnets;

[0007] A fixing frame, mounted on the main frame; one side of the fixing frame has rows of holes corresponding to the magnet, the holes are for the micro centrifuge tubes to be embedded, one side of the holes has a receiving space for receiving the micro centrifuge tubes, and the receiving space is located on the first side of the magnet;

[0008] At least two stabilizing plates, the stabilizing plates comprising a first portion and a second portion; the first portion is fixed to one side of the main frame, the second portion is located on a second side of the magnet, the second portion is used to abut against two side edges of the stage of the horizontal shaking table, and the second side is the opposite side of the first side;

[0009] The first part and the second part are connected by a damping hinge, and the second part can rotate to a set angle relative to the first part under the action of the damping hinge;

[0010] Under the action of the damping hinge, the second part can form an angle of 90 degrees or 180 degrees relative to the first part.

[0011] In an optional embodiment, when the second part is 90 degrees relative to the first part, the second part is located inside the main frame;

[0012] When the second part forms an angle of 180 degrees with respect to the first part, the second part is located outside the main frame.

[0013] In an optional embodiment, the main frame includes:

[0014] A vertical plate, wherein the magnets are arranged on the vertical plate;

[0015] A column is connected to both sides of the vertical plate; and the column is fixed to the first part.

[0016] In an optional embodiment, the main frame further includes: a connecting column, which is connected to the vertical plate and / or the vertical column; the middle portion of the fixing frame has a mounting hole, and the fixing frame is connected and fixed to the main frame by engaging the connecting column through the mounting hole.

[0017] In an optional embodiment, the connecting column is connected to the vertical plate and / or the vertical column via a supporting plate.

[0018] In an optional embodiment, the connecting column is detachably connected to the supporting plate.

[0019] In an optional implementation, the stabilizing plate is located in the middle of the main frame along a first direction, and the first direction is the axial direction of the accommodating space.

[0020] In an optional embodiment, the stabilizing plate is used to abut against a side edge of the edge of the loading platform and extend along the first direction.

[0021] The microcentrifuge tube magnetic bead splash-proof device provided in the embodiment of the present application has a fixed frame for inserting the microcentrifuge tube, uses the magnet on the main frame to absorb the magnetic beads in the microcentrifuge tube, and uses the stabilizing plate to abut against the loading platform of the horizontal shaker so that the main frame can remain stable on the horizontal shaker. With such a configuration, as the horizontal shaker is started, the magnetic bead suspension remaining at the mouth of the microcentrifuge tube can be absorbed by the magnet on the main frame, preventing the magnetic bead suspension from splashing out when the microcentrifuge tube is opened and affecting the experiment.

[0022] Additional aspects and advantages of the present application will be given in part in the description below, and in part will become apparent from the description below, or will be learned through the practice of the present application. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] The drawings described herein are used to provide a further understanding of the present application and constitute a part of the present application. The illustrative embodiments of the present application and their descriptions are used to explain the present application and do not constitute an improper limitation on the present application. In the drawings:

[0024] Figure 1 A schematic diagram of the structure of a microcentrifuge tube magnetic bead splash prevention device provided in an embodiment of the present application;

[0025] Figure 2 A schematic diagram of the structure of a stabilizing plate provided in an embodiment of the present application;

[0026] Figure 3 A schematic diagram of the unfolded state of the stabilizing plate provided in an embodiment of the present application;

[0027] Figure 4 A schematic diagram of a stabilizing plate in a retracted state provided in an embodiment of the present application;

[0028] Figure 5 A schematic diagram of the main frame structure provided in an embodiment of the present application;

[0029] Figure 6 A schematic diagram of the connection relationship of the microcentrifuge tube magnetic bead splash prevention device provided in an embodiment of the present application when placed on a horizontal shaker.

[0030] The reference numerals in the figure are:

[0031] 1. Main frame; 10. Vertical plate; 11. Magnet; 12. Vertical column; 121. Mounting slot; 13. Support plate; 14. Connecting column;

[0032] 2. Fixed frame;

[0033] 3. Mounting holes;

[0034] 4. Hole position;

[0035] 5. Stabilizing plate; 51. First part; 52. Second part; 53. Damping hinge;

[0036] 6. Horizontal shaker; 61. Sample stage. DETAILED DESCRIPTION

[0037] The exemplary embodiments disclosed in the present application will be described in more detail below with reference to the accompanying drawings. Although the exemplary embodiments of the present application are shown in the accompanying drawings, it should be understood that the present application can be implemented in various forms and should not be limited by the specific embodiments described herein. On the contrary, these embodiments are provided to enable a more thorough understanding of the present application and to fully convey the scope disclosed in the present application to those skilled in the art.

[0038] In the following description, a large number of specific details are given to provide a more thorough understanding of the present application. However, it is obvious to those skilled in the art that the present application can be implemented without one or more of these details. In other examples, in order to avoid confusion with the present application, some technical features known in the art are not described; that is, all features of the actual embodiments are not described here, and well-known functions and structures are not described in detail.

[0039] Spatial relationship terms such as "under", "below", "below", "under", "above", "above", etc., may be used here for convenience of description to describe the relationship between an element or feature shown in the figure and other elements or features. It should be understood that in addition to the orientation shown in the figure, the spatial relationship terms are intended to include different orientations of the device in use and operation. For example, if the device in the accompanying drawings is turned over, then the elements or features described as "under other elements" or "under it" or "under it" will be oriented as "on" other elements or features. Therefore, the exemplary terms "under" and "under" may include both upper and lower orientations. The device can be oriented otherwise (rotated 90 degrees or other orientations) and the spatial descriptors used herein are interpreted accordingly.

[0040] The purpose of the terms used herein is only to describe specific embodiments and is not intended to be limiting of the present application. When used herein, the singular forms "one", "an" and "said / the" are also intended to include plural forms, unless the context clearly indicates otherwise. It should also be understood that the terms "consisting of" and / or "comprising", when used in this specification, determine the presence of the features, integers, steps, operations, elements and / or parts, but do not exclude the presence or addition of one or more other features, integers, steps, operations, elements, parts and / or groups. When used herein, the term "and / or" includes any and all combinations of the relevant listed items.

[0041] In order to thoroughly understand the present application, detailed steps and detailed structures will be presented in the following description to illustrate the technical solution of the present application. The preferred embodiments of the present application are described in detail below, but in addition to these detailed descriptions, the present application may also have other implementation methods.

[0042] The present application embodiment provides a micro centrifuge tube magnetic bead splash prevention device, such as Figure 1 , Figure 2As shown, it comprises: a main frame 1, a fixing frame 2 and a stabilizing plate 5. Among them, the main frame 1 has rows of magnets 11; the fixing frame 2 is installed on the main frame 1; one side of the fixing frame 2 has rows of holes 4 arranged corresponding to the magnets 11, the holes 4 are for the micro centrifuge tubes to be embedded, and one side of the holes 4 has a receiving space for receiving the micro centrifuge tubes, and the receiving space is located on the first side of the magnet 11; there are at least two stabilizing plates 5, and the stabilizing plates 5 include a first part 51 and a second part 52; the first part 51 is fixed to one side of the main frame 1, and the second part 52 is located on the second side of the magnet 11, and the second part 52 is used to abut against the two side edges of the stage 61 of the horizontal shaking table 6, and the second side is the opposite side of the first side.

[0043] The microcentrifuge tube magnetic bead splash-proof device provided in this embodiment is applied to the separation and purification of extracellular vesicles. The magnetic bead splash-proof device provided in this embodiment supports both vertical and horizontal placement, such as Figure 1 As shown, the magnetic bead splash-proof device is placed vertically, that is, the axial direction of the storage space is along the vertical direction, that is, the axial direction of the micro-centrifuge tube placed in the storage space is also along the vertical direction, so when the magnetic bead splash-proof device is placed vertically, it is convenient to open the cover of the micro-centrifuge tube and add or remove the sample from it. Figure 6 As shown, the magnetic bead splash-proof device is in a horizontal position, that is, the axial direction of the accommodation space is in the horizontal direction, that is, the axial direction of the microcentrifuge tube placed in the accommodation space is also in the horizontal direction, so the lid of the microcentrifuge tube needs to be closed when the magnetic bead splash-proof device is in a horizontal position. The specific working process is taken as an example (it needs to be used with a vortex oscillator, a pipette, a horizontal shaker 6 and an extraction kit):

[0044] 1. Pipette 1 mL of pretreated plasma or cell supernatant into a 1.5 mL microcentrifuge tube;

[0045] 2. Add 200 μL of magnetic bead suspension to the microcentrifuge tube and mix well using a vortex oscillator;

[0046] 3. After vortexing, transfer the microcentrifuge tube to a flip mixer and incubate at room temperature for 30 minutes;

[0047] 4. After the incubation is completed, the microcentrifuge tube is inserted into the hole 4 of the magnetic bead splash-proof device, so that the microcentrifuge tube is accommodated in the accommodation space, and the magnet 11 is closely fitted with the microcentrifuge tube. Figure 6 As shown, the magnetic bead splash-proof device is then placed at the upper limit of the stage 61 of the horizontal shaker 6. The power of the horizontal shaker 6 is then turned on to perform magnetic bead adsorption and separation for 3 minutes. As the stage 61 of the horizontal shaker is repeatedly shaken, the magnetic bead suspension adsorbed on the tube mouth of the microcentrifuge tube due to vortexing and flipping incubation and the magnetic bead suspension in the tube are fully adsorbed on the wall of the microcentrifuge tube by the magnet 11;

[0048] 5. After the magnetic bead adsorption and separation is completed, the supernatant in the microcentrifuge tube is discarded with a pipette, 1 mL of washing solution is added, and after mixing and washing with a vortex oscillator, the microcentrifuge tube is inserted into the hole 4 of the magnetic bead splash-proof device so that it is accommodated in the accommodation space, and the magnet 11 is closely attached to the microcentrifuge tube. Figure 6 As shown, the magnetic bead splash-proof device is then placed at the upper limit of the stage 61 of the horizontal shaker 6. The power of the horizontal shaker 6 is then turned on to perform magnetic bead adsorption and separation for 3 minutes. As the stage 61 of the horizontal shaker is repeatedly shaken, the magnetic bead suspension adsorbed on the tube mouth of the microcentrifuge tube due to vortexing and flipping incubation and the magnetic bead suspension in the tube are fully adsorbed on the wall of the microcentrifuge tube by the magnet 11;

[0049] 6. Repeat step 5 as many times as you need to wash;

[0050] 7. After the magnetic bead adsorption and separation is completed, use a pipette to discard the supernatant in the microcentrifuge tube and add 200 μL of elution solution, use a vortex oscillator to mix, incubate at room temperature for 5 minutes, and vortex oscillate 3 times during the incubation period;

[0051] 8. After the incubation is completed, insert the microcentrifuge tube into the hole 4 of the magnetic bead splash-proof device, and then position the magnetic bead splash-proof device at the upper limit of the stage 61 of the horizontal shaker 6. Turn on the power of the horizontal shaker 6 and perform magnetic bead adsorption and separation for 3 minutes, so that the magnetic bead suspension adsorbed on the tube mouth of the microcentrifuge tube due to the vortex and the magnetic bead suspension in the tube are fully adsorbed on the wall of the microcentrifuge tube;

[0052] 9. After the magnetic bead adsorption and separation is completed, use a pipette to transfer the supernatant in the microcentrifuge tube and at the tube mouth to a new microcentrifuge tube to obtain purified extracellular vesicles, and store them at -20°C for later use.

[0053] The step of limiting the magnetic bead splash-proof device on the stage 61 of the horizontal shaker 6 is as follows: the second part 52 of the stabilizing plate 5 is directed toward the stage 61 of the horizontal shaker, and the second parts 52 on both sides of the main frame 1 are respectively abutted against the edges of both sides of the stage 61, so that the magnetic bead splash-proof device for the micro-centrifuge tube provided in this embodiment has a certain limit on the stage 61. After this step is completed, the axial direction of the micro-centrifuge tube in the hole position 4 is all extended in the horizontal direction or close to the horizontal direction, and the magnet 11 is located at the lower side of the micro-centrifuge tube.

[0054] The microcentrifuge tube magnetic bead splash-proof device provided in the embodiment of the present application has a fixing frame 2 for inserting the microcentrifuge tube, uses the magnet 11 on the main frame 1 to absorb the magnetic beads in the microcentrifuge tube, and uses the stabilizing plate 5 to abut against the loading table 61 of the horizontal shaker 6, so that the main frame 1 can remain stable on the horizontal shaker 6. With such a configuration, as the horizontal shaker 6 is started, the magnetic bead suspension remaining at the mouth of the microcentrifuge tube can be absorbed by the magnet 11 on the main frame 1, avoiding the magnetic bead suspension from splashing out when the microcentrifuge tube is opened and affecting the experiment, which can reduce the loss of samples and prevent splashing pollution.

[0055] In an optional embodiment, the microcentrifuge tube magnetic bead splash prevention device of this embodiment, such as Figure 2 As shown, the first part 51 and the second part 52 are connected by a damping hinge 53, and the second part 52 can rotate to a set angle relative to the first part 51 under the action of the damping hinge 53. The function of the damping hinge 53 is to keep the second part 52 at the set angle, so that the structure of the second part 52 is stable when it abuts against the stage 61, and the limit provided to the magnetic bead splash-proof device will be more stable, ensuring that the magnetic bead splash-proof device will not overturn when the stage 61 shakes.

[0056] In an optional embodiment, the microcentrifuge tube magnetic bead splash prevention device of this embodiment, such as Figure 3 , Figure 4 As shown, the second part 52 can form an angle of 90 degrees or 180 degrees relative to the first part 51 under the action of the damping hinge 53. In this solution, two switchable angles are set by the damping hinge 53, so that the second part 52 is easy to adjust. Figure 3 As shown, the stabilizing plate 5 is unfolded, and the second portion 52 forms an angle of 180 degrees relative to the first portion 51; Figure 4 As shown, the stabilizing plate 5 is folded, and the second portion 52 forms a 90-degree angle with respect to the first portion 51. When the stabilizing plate 5 is unfolded, the microcentrifuge tube magnetic bead splash-proof device of this embodiment can be placed on the stage 61 of the horizontal shaking table 6, as shown in FIG. Figure 6 As shown, when the stabilizing plate 5 is folded, the second portion 52 rotates 90 degrees and can be buckled on both sides of the loading platform 61, so as to firmly connect the main frame 1 and the loading platform 61, thereby achieving a significant stabilizing effect.

[0057] In an optional embodiment, the microcentrifuge tube magnetic bead splash prevention device of this embodiment, such as Figure 3 , Figure 4 As shown, when the second portion 52 is 90 degrees relative to the first portion 51, the second portion 52 is located inside the main frame 1;

[0058] When the second portion 52 forms an angle of 180 degrees with respect to the first portion 51 , the second portion 52 is located outside the main frame 1 .

[0059] In this solution, when the second part 52 is located on the inner side of the main frame 1, the microcentrifuge tube magnetic bead splash-proof device of this embodiment occupies a small space as a whole, that is, the stabilizing plate 5 does not occupy additional space when it is folded. Only when the stabilizing plate 5 is needed, the stabilizing plate 5 is selectively unfolded to abut the loading platform 61, thereby improving the convenience of using the magnetic bead splash-proof device.

[0060] In an optional embodiment, if Figure 5 As shown, the main frame 1 includes: a vertical plate 10, a column 12 and a connecting column 14. Among them, the magnet 11 is arranged on the vertical plate 10; the column 12 is connected to both sides of the vertical plate 10; and the column 12 is fixed to the first part 51. In this solution, the vertical plate 10, the column 12 and the connecting column 14 have simple structures and are easy to manufacture. It is easy to make a groove on the vertical plate 10 for the magnet 11 to be embedded and fixed; the column 12 can provide a stable supporting force.

[0061] In an optional embodiment, if Figure 5 As shown, the main frame 1 also includes a connecting column 14, which is connected to the vertical plate 10 and / or the vertical column 12. The middle part of the fixing frame 2 has a mounting hole 3, and the fixing frame 2 is connected and fixed to the main frame 1 through the mounting hole 3 by engaging the connecting column 14. In this solution, the fixing frame 2 can be easily connected or separated from the main frame 1 through the connecting column 14, so that the fixing frame 2 has enough operating space to facilitate the microcentrifuge tube to be loaded into the hole 4.

[0062] In an optional embodiment, if Figure 5 As shown, the connecting column 14 is connected to the vertical plate 10 and / or the column 12 through the support plate 13. The support plate 13 is connected to the vertical plate 10 and / or the column 12, and the structure is stable. The connecting column 14 is connected to the support plate 13 to obtain sufficient support, which is conducive to improving the reliability of the connection with the fixing frame 2.

[0063] In an optional embodiment, the connecting column 14 is detachably connected to the support plate 13. This structure can improve the convenience of connecting the fixing frame 2 and the main frame 1. For example, the connection between the fixing frame 2 and the main frame 1 can be quickly completed by aligning the corresponding holes of the fixing frame 2 and the main frame 1, and then inserting the connecting column 14 between the two to fix them.

[0064] In an optional embodiment, the microcentrifuge tube magnetic bead splash-proof device of this embodiment, the stabilizing plate 5 is located in the middle of the main frame 1 along the first direction, and the first direction is the axial direction of the accommodation space. In this scheme, the stabilizing plate 5 is centrally arranged, which can make the center of gravity of the microcentrifuge tube magnetic bead splash-proof device of this embodiment more stable when placed on the stage 61, and can prevent the device from tipping over. Figure 5 As shown, a mounting groove 121 for embedding and mounting the first portion 51 of the stabilizing plate 5 is provided in the middle of the main frame 1 along the first direction.

[0065] In an optional embodiment, the stabilizing plate 5 extends along the first direction at one side edge thereof abutting against the edge of the stage 61. In this solution, the stabilizing plate 5 and the stage 61 have a greater contact length in the first direction, which can improve the stability of the connection between the stabilizing plate 5 and the stage 61.

[0066] It should be understood that the above embodiments are exemplary and are not intended to include all possible implementations included in the claims. Various modifications and changes may be made on the basis of the above embodiments without departing from the scope of the present disclosure. Similarly, the various technical features of the above embodiments may be arbitrarily combined to form other embodiments of the present application that may not be explicitly described. Therefore, the above embodiments only express several implementations of the present application and do not limit the scope of protection of the patent of this application.

Claims

1. A microcentrifuge tube magnetic bead splash-proof device, characterized in that: include: A main frame (1), wherein the main frame (1) has rows of magnets (11); A fixing frame (2) is mounted on the main frame (1); one side of the fixing frame (2) has rows of holes (4) arranged corresponding to the magnet (11), the holes (4) are for the micro centrifuge tubes to be inserted, one side of the holes (4) has a receiving space for receiving the micro centrifuge tubes, and the receiving space is located on a first side of the magnet (11); at least two stabilizing plates (5), the stabilizing plates (5) comprising a first portion (51) and a second portion (52); the first portion (51) is fixed to one side of the main frame (1), the second portion (52) is located on a second side of the magnet (11), the second portion (52) is used to abut against two side edges of a loading platform (61) of a horizontal shaking table (6), the second side being an opposite side to the first side; The first part (51) and the second part (52) are connected via a damping hinge (53), and the second part (52) can rotate to a set angle relative to the first part (51) under the action of the damping hinge (53); Under the action of the damping hinge (53), the second part (52) can form an angle of 90 degrees or 180 degrees relative to the first part (51).

2. The microcentrifuge tube magnetic bead splash-proof device according to claim 1, characterized in that: When the second part (52) is at an angle of 90 degrees relative to the first part (51), the second part (52) is located inside the main frame (1); When the second part (52) forms an angle of 180 degrees with respect to the first part (51), the second part (52) is located outside the main frame (1).

3. The microcentrifuge tube magnetic bead splash-proof device according to claim 1 or 2, characterized in that: The main frame (1) comprises: A vertical plate (10), wherein the magnets (11) are arranged on the vertical plate (10); A column (12), the column (12) being connected to two sides of the vertical plate (10); the column (12) being fixed to the first part (51).

4. The microcentrifuge tube magnetic bead splash-proof device according to claim 3, characterized in that: The main frame (1) further comprises: a connecting column (14), wherein the connecting column (14) is connected to the upright plate (10) and / or the upright column (12); a mounting hole (3) is provided in the middle portion of the fixing frame (2), and the fixing frame (2) is connected and fixed to the main frame (1) by engaging the connecting column (14) through the mounting hole (3).

5. The microcentrifuge tube magnetic bead splash-proof device according to claim 4, characterized in that: The connecting column (14) is connected to the vertical plate (10) and / or the vertical column (12) via a supporting plate (13).

6. The microcentrifuge tube magnetic bead splash-proof device according to claim 5, characterized in that: The connecting column (14) is detachably connected to the supporting plate (13).

7. The microcentrifuge tube magnetic bead splash-proof device according to claim 1 or 2, characterized in that: The stabilizing plate (5) is located in the middle of the main frame (1) along a first direction, wherein the first direction is the axial direction of the accommodating space.

8. The microcentrifuge tube magnetic bead splash prevention device according to claim 7, characterized in that: The stabilizing plate (5) is used to abut against one side edge of the edge of the object carrier (61) and extend along the first direction.

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