Gradient protein renaturation device

Abstract

The utility model discloses a gradual change type protein renaturation device, which relates to the technical field of protein renaturation, and comprises a barrel body, a sealing door is movably arranged in a manner of penetrating through the front part of the barrel body, a positioning assembly is arranged in the barrel body close to the bottom end, a mixing assembly is arranged in the barrel body close to the top end, the positioning assembly comprises a supporting seat and a container, the supporting seat is fixedly installed at the position, close to the bottom end, in the barrel body, a hydraulic cylinder is installed in the supporting seat, a chassis is installed at the top end of the hydraulic cylinder, a positioning groove is formed in the top of the chassis, and positioning rods are movably installed in the positioning groove and penetrate through the two sides of the positioning groove; a sliding block is installed at the tail end, close to the bottom, of the positioning rod, and a first spring is installed on the periphery of the positioning rod. The container is convenient to mount and dismount, is convenient to replace and clean the interior for next use, and can gradually reduce the concentration of liquid, so that the liquid is fully mixed, and the protein renaturation efficiency is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of protein refolding, in particular to a gradual protein refolding device. Background Art

[0002] Proteins are widely present in cells, tissues, and organs, and they undertake a variety of physiological functions. Protein renaturation refers to the restoration of the original natural conformation of proteins under specific conditions. This scheme specifically relates to a gradual protein renaturation device;

[0003] When using the existing protein refolding device, the liquid easily adheres to the inner wall of the container during the refolding process, which makes it inconvenient to disassemble and assemble the container and clean the inside, making it inconvenient for the next use. In addition, the refolding efficiency is low, it is not easy to stir, and the concentration of the dialysate is reduced, which has a certain impact on actual use. Utility Model Content

[0004] The main purpose of the utility model is to provide a gradual protein renaturation device, which can effectively solve the technical problems in the background technology.

[0005] In order to achieve the above purpose, the technical solution adopted by the utility model is:

[0006] A gradual protein refolding device comprises a barrel body, a sealing door is movably installed through the front of the barrel body, a positioning assembly is installed inside the barrel body near the bottom, a mixing assembly is installed inside the barrel body near the top, the positioning assembly comprises a support seat and a container, the support seat is fixedly installed inside the barrel body near the bottom, a hydraulic cylinder is installed inside the support seat, a chassis is installed on the top of the hydraulic cylinder, a positioning groove is opened on the top of the chassis, a positioning rod is movably installed inside the positioning groove and on both sides of the positioning groove, a sliding block is installed at the end of the positioning rod near the bottom, a first spring is installed on the periphery of the positioning rod, a positioning block is fixedly installed on the bottom of the container, positioning holes are opened on both sides of the positioning block, a push plate is movably installed inside the chassis and on the rear part of the positioning groove, and a second spring is installed at the rear of the push plate.

[0007] As a further solution of the utility model, the front surface of the support seat is conical, the chassis is fixed to the top of the telescopic rod of the hydraulic cylinder, and a drainage pipe is installed on the outside of the barrel body near the bottom.

[0008] As a further solution of the utility model, the surface of the inner end of the positioning rod is inclined, and the two positioning rods are symmetrically arranged.

[0009] As a further solution of the utility model, the positioning block is slidably arranged in the positioning groove, and the inner end of the positioning rod is snap-connected with the positioning hole.

[0010] As a further solution of the utility model, the mixing assembly includes an annular tube, which is fixedly installed inside the barrel body near the top, a liquid inlet pipe is installed on the periphery of the annular tube and passes through the outside of the barrel body and is connected to the top, four conduits are fixedly installed on the bottom of the annular tube, a holder is fixedly installed on the outside of the conduits near the middle, a motor is installed on the top of the barrel body, and a stirring rod is installed on the bottom end of the output shaft of the motor.

[0011] As a further solution of the utility model, the conduits are connected to the inside of the annular tube, and the four conduits are arranged in a ring shape.

[0012] As a further solution of the utility model, the diameter of the annular tube is smaller than the diameter of the container, and the stirring rod and the output shaft of the motor are arranged to rotate synchronously.

[0013] The beneficial effects of the utility model are as follows:

[0014] By setting the positioning assembly, the positioning block slides into the positioning groove, and the container is fixed to the chassis through the clamping connection between the positioning rod and the positioning hole, which is convenient for installation and removal of the container, and convenient for replacement and cleaning of the interior. The push plate slides the positioning block forward under the action of the second spring, so that the positioning hole and the positioning rod are misaligned, which is convenient for automatic pop-up to take and place the container;

[0015] By setting up a mixing component, the container is raised and lowered by a hydraulic cylinder, the top of the container is connected to the holder, and the diluent is transported to the container through a catheter to gradually reduce the liquid concentration. By rotating the stirring rod, the concentration of the dialysate is reduced faster and the refolding efficiency of the protein is improved. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 This is a schematic diagram of the overall structure of a gradual protein refolding device of the utility model;

[0017] Figure 2 This is a schematic diagram of the internal structure of a positioning component in a gradual protein refolding device of the utility model;

[0018] Figure 3 This is a schematic diagram of the internal structure of a chassis in a gradual protein refolding device of the utility model;

[0019] Figure 4 The utility model is a schematic diagram of the structure of a mixing component in a gradual protein refolding device.

[0020] In the figure: 1. barrel; 2. sealing door; 3. positioning assembly; 4. mixing assembly; 5. support seat; 6. container; 7. hydraulic cylinder; 8. chassis; 9. positioning groove; 10. positioning rod; 11. slider; 12. first spring; 13. positioning block; 14. positioning hole; 15. push plate; 16. second spring; 17. annular tube; 18. liquid inlet pipe; 19. conduit; 20. holder; 21. motor; 22. stirring rod. DETAILED DESCRIPTION

[0021] In order to make the technical means, creative features, objectives and effects achieved by the present invention easy to understand, the present invention is further described below in conjunction with specific implementation methods.

[0022] like Figure 1-4 As shown, a gradual protein refolding device comprises a barrel body 1, a sealing door 2 is movably installed through the front of the barrel body 1, a positioning assembly 3 is installed inside the barrel body 1 near the bottom, a mixing assembly 4 is installed inside the barrel body 1 near the top, the positioning assembly 3 comprises a support seat 5 and a container 6, the support seat 5 is fixedly installed inside the barrel body 1 near the bottom, a hydraulic cylinder 7 is installed inside the support seat 5, a chassis 8 is installed at the top of the hydraulic cylinder 7, a positioning groove 9 is provided on the top of the chassis 8, a positioning rod 10 is movably installed inside the positioning groove 9 and on both sides of the positioning groove 9, a slider 11 is installed at the end of the positioning rod 10 near the bottom, a first spring 12 is installed on the periphery of the positioning rod 10, a positioning block 13 is fixedly installed at the bottom of the container 6, and positioning holes 14 are provided on both sides of the positioning block 13, a push plate 15 is movably installed inside the chassis 8 and on the rear of the positioning groove 9, and a second spring 16 is installed at the rear of the push plate 15.

[0023] In this embodiment, the front view surface of the support seat 5 is conical, the chassis 8 is fixed to the top of the telescopic rod of the hydraulic cylinder 7, and a drainage pipe is installed on the outside of the barrel body 1 near the bottom. The chassis 8 is raised and lowered by extending and retracting the telescopic rod in the hydraulic cylinder 7 to carry out the taking and placing of the container 6.

[0024] In this embodiment, the inner end surface of the positioning rod 10 is inclined, and the two positioning rods 10 are symmetrically arranged. The positioning rods 10 slide inward through the first spring 12 and engage with the positioning hole 14 to fix the container 6 and the chassis 8 to each other.

[0025] In this embodiment, the positioning block 13 is located in the positioning groove 9 and is slidably arranged. The inner end of the positioning rod 10 and the positioning hole 14 are snap-connected. The positioning block 13 is snap-connected in the positioning groove 9. The positioning rod 10 and the positioning hole 14 are snap-connected or disengaged by sliding the slider 11, which is convenient for taking and placing the container 6.

[0026] In this embodiment, the mixing assembly 4 includes an annular tube 17, which is fixedly installed inside the barrel body 1 near the top, and an inlet pipe 18 is installed on the periphery of the annular tube 17 and passes through the outside of the barrel body 1 and is connected to the top, four conduits 19 are fixedly installed at the bottom of the annular tube 17, and a holder 20 is fixedly installed on the outside of the conduit 19 near the middle, a motor 21 is installed on the top of the barrel body 1, and a stirring rod 22 is installed at the bottom end of the output shaft of the motor 21. The diluent is continuously transported to the container 6 through the conduit 19 to accelerate the reduction of the dialysate concentration.

[0027] In this embodiment, the conduits 19 are interconnected with the inside of the annular tube 17 . The four conduits 19 are arranged in a ring shape. By connecting the liquid inlet pipe 18 to the liquid storage tank, the diluent is transported to the four conduits 19 through the annular tube 17 .

[0028] In this embodiment, the diameter of the annular tube 17 is smaller than the diameter of the container 6 , the stirring rod 22 is arranged to rotate synchronously with the output shaft of the motor 21 , the top of the container 6 is engaged with the inner walls of the four holders 20 , and stirring and mixing are performed by the rotation of the stirring rod 22 .

[0029] It should be noted that the utility model is a gradual protein renaturation device. When in use, the protein dialysis bag to be renatured is placed in the container 6. When fixing it, the positioning block 13 at the bottom of the container 6 is slid into the positioning groove 9. The positioning block 13 contacts the inclined surface of the inner end of the positioning rod 10, so that the positioning rod 10 slides outward. When the positioning block 13 is fully inserted, the positioning rod 10 is automatically inserted into the positioning hole 14 under the action of the first spring 12, thereby fixing the container 6 and the chassis 8 to each other. When it is taken out for replacement, the sealing door 2 is opened and the two sliders 11 are pushed outward to make the positioning rod 10 slide out of the positioning hole 14, and Under the action of the second spring 16, the push plate 15 slides forward to push out the positioning block 13, so that the positioning hole 14 and the positioning rod 10 are misaligned, and the container 6 is taken out for the next protein renaturation. During the renaturation, the hydraulic cylinder 7 is started to make the chassis 8 drive the container 6 to rise, and the top of the container 6 is engaged with the card seat 20 for fixing, and the liquid inlet pipe 18 is connected. The diluent enters the four conduits 19 through the ring pipe 17 and is transported to the container 6. The stirring rod 22 is rotated by starting the motor 21 to accelerate the reduction of the concentration of the dialysate. At the same time, the overflowed liquid enters the bottom end of the barrel body 1 and is discharged through the discharge pipe to obtain the renatured protein.

[0030] The above shows and describes the basic principle and main features of the utility model and the advantages of the utility model. Those skilled in the art should understand that the utility model is not limited by the above embodiments. The above embodiments and descriptions are only for explaining the principle of the utility model. Without departing from the spirit and scope of the utility model, the utility model may have various changes and improvements, which fall within the scope of the utility model to be protected. The scope of protection claimed by the utility model is defined by the attached claims and their equivalents.

Claims

1. A gradual protein refolding device, comprising a barrel (1), a sealing door (2) movably installed through the front of the barrel (1), a positioning component (3) installed near the bottom of the barrel (1), and a mixing component (4) installed near the top of the barrel (1), characterized in that: The positioning assembly (3) comprises a support seat (5) and a container (6). The support seat (5) is fixedly mounted inside the barrel body (1) near the bottom. A hydraulic cylinder (7) is mounted inside the support seat (5). A chassis (8) is mounted on the top of the hydraulic cylinder (7). A positioning groove (9) is provided on the top of the chassis (8). A positioning rod (10) is movably mounted inside the positioning groove (9) and on both sides of the positioning groove (9). A slider (11) is mounted at the bottom of the end of the positioning rod (10). A first spring (12) is mounted on the periphery of the positioning rod (10). A positioning block (13) is fixedly mounted on the bottom of the container (6). Positioning holes (14) are mounted on both sides of the positioning block (13). A push plate (15) is movably mounted inside the chassis (8) and on the rear of the positioning groove (9). A second spring (16) is mounted on the rear of the push plate (15).

2. A gradual protein renaturation device according to claim 1, characterized in that: The front surface of the support seat (5) is arranged in a conical shape, the chassis (8) is fixed to the top end of the telescopic rod of the hydraulic cylinder (7), and a drainage pipe is installed on the outside of the barrel body (1) near the bottom end.

3. A gradual protein renaturation device according to claim 1, characterized in that: The inner end surface of the positioning rod (10) is arranged in an inclined manner, and the two positioning rods (10) are arranged symmetrically to each other.

4. A gradual protein renaturation device according to claim 1, characterized in that: The positioning block (13) is slidably arranged in the positioning groove (9), and the inner end of the positioning rod (10) is clamped with the positioning hole (14).

5. A gradual protein renaturation device according to claim 1, characterized in that: The mixing assembly (4) comprises an annular tube (17), the annular tube (17) being fixedly mounted inside the barrel body (1) near the top end, a liquid inlet tube (18) being mounted on the outer periphery of the annular tube (17) and penetrating through the outer periphery of the barrel body (1) near the top end thereof, four guide tubes (19) being fixedly mounted on the bottom of the annular tube (17), a holder (20) being fixedly mounted on the outer periphery of the guide tube (19) near the middle, a motor (21) being mounted on the top of the barrel body (1), and a stirring rod (22) being mounted on the bottom end of the output shaft of the motor (21).

6. A gradual protein renaturation device according to claim 5, characterized in that: The conduits (19) are connected to the interior of the annular tube (17), and the four conduits (19) are arranged in a ring shape.

7. A gradual protein renaturation device according to claim 5, characterized in that: The diameter of the annular tube (17) is smaller than the diameter of the container (6), and the stirring rod (22) is arranged to rotate synchronously with the output shaft of the motor (21).

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