Microorganism culture medium manufacturing and quantitative subpackaging integrated device

Abstract

The utility model relates to a microbial culture medium manufacturing and quantitative subpackaging integrated device which comprises a base (1), a liquid separator (2), a controller (3) and a machine body (4), wherein the liquid separator (2) is used for quantitatively sub-packaging a culture medium; the machine body (4) is arranged on the base (1) and is connected with the liquid separator (2); the machine body (4) comprises a hollow bottomed first cylinder and a hollow bottomed second cylinder, the second cylinder is arranged in the first cylinder, and the diameter and the height of the second cylinder are smaller than those of the first cylinder; the first cylinder is used for manufacturing and loading a culture medium, and an electric heating wire and a liquid outlet (401) are arranged at the bottom of the first cylinder; the second cylinder is used for loading solid insoluble substances when the culture medium is manufactured, and filtering holes are distributed in the inner wall, the outer wall and the bottom of the second cylinder. The device can prevent solid insoluble substances in the culture medium from blocking the pipeline of the liquid separator, and can realize the integration of standardized manufacturing and quantitative split charging of the microbial culture medium.

Description

Technical Field

[0001] The utility model relates to the technical field of microbial culture medium subpackaging, and more specifically to a device integrating microbial culture medium production and quantitative subpackaging. Background Art

[0002] In microbial science experiments and applied microbial production, it is usually necessary to prepare a large amount of culture medium for observing microorganisms or for propagating microorganisms and obtaining their metabolites. Among them, the packaging of culture medium (including liquid culture medium and agar solid culture medium) used to culture microorganisms is an important part of the experimental process. At present, in laboratories, manual packaging is usually used after the culture medium is prepared. However, the temperature of the culture medium is high during filling, which can easily scald the experimenter. If the temperature of the culture medium is low during filling, it is easy to cause the culture medium to solidify during the packaging process, and it needs to be heated and dissolved again before packaging. The operation is time-consuming and laborious, and it is easy to cause uneven packaging, and it may even be contaminated by other microorganisms or impurities in the environment, affecting the experimental results.

[0003] Although patent CN 215103285 U is a microbial culture medium packaging device that can realize quantitative packaging of culture medium, the packaging is single, it is difficult to achieve high-throughput packaging, and the packaging efficiency is low; and the raw materials of microbial culture medium generally include soluble components such as glucose, peptone, etc. and insoluble components such as peeled potato pieces, sawdust, etc. If these insoluble solids are mixed in the culture medium, it is easy to block the liquid outlet and the liquid outlet pipe, and the residual culture medium is difficult to clean after solidification in the liquid outlet pipe. In addition, the device cannot realize the integration of culture medium production and packaging, and may be contaminated by the external environment during the process of transporting the culture medium to the sterile bag. Therefore, a device that can realize the integration of standardized production and quantitative packaging of culture medium is studied, which is conducive to ensuring the consistency of color and nutrition of different batches of culture medium, and can perform high-throughput packaging to improve the production efficiency of culture medium. Utility Model Content

[0004] In view of the above defects or improvement needs of the prior art, the utility model provides a device for integrating the preparation and quantitative packaging of microbial culture medium, wherein the device comprises a base 1, a liquid dispenser 2, a controller 3 and a body 4; wherein the liquid dispenser 2 is used for quantitatively packaging the culture medium; the body 4 is arranged on the base 1 and connected to the liquid dispenser 2;

[0005] The body 4 includes a first hollow cylinder 406 and a second cylinder 404, wherein the second cylinder is arranged in the first cylinder, and its diameter and height are smaller than the first cylinder; the first cylinder is used for making and loading culture medium, and a heating wire and a liquid outlet 401 are arranged at the bottom;

[0006] The second cylinder is used to load solid insoluble matter when preparing culture medium, and filtering holes are distributed on the inner and outer walls and the bottom of the second cylinder.

[0007] Preferably, in the device for integrating the preparation and quantitative packaging of the microbial culture medium, the bottom of the first cylinder is also provided with a stainless steel filter screen with mesh holes smaller than the filter holes of the second cylinder for secondary filtration.

[0008] Preferably, in the device for integrating the preparation and quantitative packaging of microbial culture medium, the first cylinder and the second cylinder are detachably connected; the stainless steel filter screen is circular, the diameter of which matches that of the first cylinder, and is detachably installed.

[0009] Preferably, in the device integrating the preparation and quantitative packaging of the microbial culture medium, the first cylinder has a liquid level indicator 403 on its inner wall; the second cylinder has a mechanical buckle on its top end for fixing on the first cylinder.

[0010] Preferably, the device for integrating the preparation and quantitative packaging of the microbial culture medium, wherein the liquid dispenser 2 has a liquid inlet end arranged inside the base and a liquid outlet end arranged on the side of the base, and specifically includes a solenoid valve 201, a liquid inlet pipe 202, a peristaltic pump 203, a liquid outlet pipe 204 and a nozzle 207, wherein the upper end of the liquid inlet pipe 202 is connected to the solenoid valve 201, and the lower end is connected to the liquid outlet pipe 204 through the peristaltic pump 203, and a nozzle 207 is provided at the end of the liquid outlet pipe 204.

[0011] Preferably, in the device integrating the preparation and quantitative packaging of the microbial culture medium, the liquid inlet pipe 202 and / or the liquid outlet pipe 204 are provided with electric heating wires for heat preservation during the packaging process of the culture medium.

[0012] Preferably, the device for integrating the preparation and quantitative packaging of the microbial culture medium, wherein the liquid dispenser 2 also includes a liquid separator valve 205 and a liquid outlet branch pipe 206, wherein one end of the liquid outlet pipe 204 is connected to a plurality of liquid outlet branch pipes 206 through a multi-way liquid separator valve, and a nozzle 207 is installed at the end of the liquid outlet branch pipe 206, and the liquid separator valve 205 is used to control the opening and closing of the liquid outlet branch pipe.

[0013] Preferably, the device for integrating the preparation and quantitative packaging of the microbial culture medium further comprises a stirrer 5 which is disposed in the first cylinder.

[0014] Preferably, in the integrated device for preparing and quantitatively dispensing microbial culture medium, the stirring part of the agitator 5 is located below the second cylinder, and comprises an axle 501, a purlin 502, a bearing 503, a motor 504 and an impeller 505, wherein the purlin is connected to the bearing to form an outer ring, and the diameter of the outer ring matches the diameter of the first cylinder, so as to fix the agitator on the first cylinder.

[0015] Preferably, the device for integrating the preparation and quantitative packaging of the microbial culture medium also includes a cover for preventing the solid insoluble matter in the second cylinder from overflowing.

[0016] In general, the above technical solutions conceived by the utility model can achieve the following beneficial effects compared with the prior art:

[0017] The utility model provides an integrated device for the preparation and quantitative packaging of a microbial culture medium, comprising a base 1, a dispenser 2, a controller 3 and a body 4, wherein the body comprises a first cylinder and a second cylinder, the first cylinder being used to load the prepared culture medium, the second cylinder being used to load solid insolubles, and since the second cylinder is a cylinder with filter holes, the nutrients dissolved from the solid components can enter the first cylinder while the remaining insoluble components are retained in the second cylinder, thereby preventing the culture fluid from being mixed with insolubles and clogging the dispenser pipeline. The dispenser is controlled by a controller, and can quantitatively package the culture medium according to the design parameters and can perform high-throughput quantitative packaging, which is beneficial to improving the efficiency of culture medium packaging. Compared with existing culture medium packaging devices, this device can realize the integration of standardized culture medium preparation and quantitative packaging. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 is a schematic diagram of the integrated device in Example 1;

[0019] Figure 1 1: base, 2: dispenser, 3: controller, 4: machine body, 5: stirrer;

[0020] Figure 2 is a schematic diagram of the liquid dispenser 2 in Example 1;

[0021] Figure 2 201: solenoid valve, 202: liquid inlet pipe, 203: peristaltic pump, 204: liquid outlet pipe, 205: liquid separation valve, 206: liquid outlet branch pipe, 207: nozzle;

[0022] Figure 3 is a schematic diagram of the machine body 4 in Embodiment 1;

[0023] Figure 3 401: liquid outlet, 402: screen, 403: liquid level indicator, 404: second cylinder, 405: buckle; 406: first cylinder;

[0024] Figure 4 is a schematic diagram of the agitator 5 in Example 1;

[0025] Figure 4Among them, 501: axle, 502: purlin, 503: bearing, 504: motor, 505: impeller. DETAILED DESCRIPTION

[0026] In order to further explain the technical means and effects adopted by the present invention to achieve the predetermined purpose of the utility model, the specific implementation method, structure, characteristics and effects of the present invention are described in detail below in combination with the accompanying drawings and preferred embodiments.

[0027] The utility model provides an integrated device for preparing microbial culture medium and quantitative packaging, which comprises a base 1, a dispenser 2, a controller 3 and a body 4, wherein the controller 3 is arranged on the side of the base 1, and is used to control the heating and heat preservation of the body and the heat preservation and quantitative packaging of the dispenser; the dispenser 2 is used for quantitative packaging of culture medium, and its liquid inlet end is arranged inside the base, and the liquid outlet is arranged on the side of the base; the body 4 is arranged above the base and is connected to the liquid inlet end of the dispenser.

[0028] The body 4 includes a first hollow cylinder 406 with a bottom and a second cylinder 404, wherein the second cylinder is arranged in the first cylinder, and its diameter and height are smaller than the first cylinder.

[0029] The first cylinder is provided with a vacuum insulation function, and is mainly used to accommodate the prepared culture medium. A heating wire is provided at the bottom thereof, which is used to heat the body to dissolve the culture medium raw materials. A liquid outlet 401 is provided at the bottom, and the liquid outlet 401 is connected to the liquid inlet end of the dispenser. The heating wire is controlled by a controller. Preferably, a liquid level indicator 403 is provided on the inner wall of the first cylinder to facilitate the control of the amount of water added during the preparation of the culture medium, which is beneficial to reducing the difference in the quality of the culture medium between different batches.

[0030] The second cylinder is used to load solid insoluble substances such as potato pieces, sawdust, etc. added when making the culture medium. The inner and outer walls and the bottom are provided with filtering holes, which are used to release the nutrients dissolved from the solid insoluble substances into the first cylinder to form a liquid culture medium while the insoluble components are retained in the second cylinder to avoid clogging the pipe of the liquid dispenser. Preferably, the first cylinder and the second cylinder are detachably connected. For example, in some embodiments, the top of the second cylinder is provided with a mechanical buckle such as buckle 405 for fixing with the first cylinder.

[0031] More preferably, a stainless steel filter screen is installed at the bottom of the first cylinder. The stainless steel filter screen is circular, its diameter matches the first cylinder, and its mesh size is smaller than the filter hole of the second cylinder. It is used for secondary filtration to prevent the insoluble matter mixed in the culture medium from clogging the pipe of the dispenser. It is preferably a detachable stainless steel filter screen. In some embodiments, the filter hole of the second cylinder is 10 mesh, and the stainless steel filter screen is 50 mesh, which is detachably installed with the first cylinder.

[0032] In some embodiments, the dispenser 2 includes a solenoid valve 201, a liquid inlet tube 202, a peristaltic pump 203, a liquid outlet tube 204 and a nozzle 207, and is used for quantitatively dispensing culture medium, wherein the upper end of the liquid inlet tube 202 is connected to the solenoid valve 201, and the lower end is connected to the liquid outlet tube 204 through the peristaltic pump 203, and a nozzle 207 is provided at the end of the liquid outlet tube 204. The dispenser 2 is connected to the liquid outlet at the bottom of the first cylindrical body of the machine body through an electromagnetic valve. The device controls the electromagnetic valve 201 and the peristaltic pump 203 through a controller to realize the start and stop of the electromagnetic valve and the infusion of the peristaltic pump; preferably, the liquid inlet pipe 202 and the liquid outlet pipe 204 are provided with heating wires to maintain the temperature of the culture medium during the packaging process to avoid low-temperature solidification, and at the same time, it is also beneficial to heat and dissolve the solidified residual culture medium in the later stage, so that the cleaning is more thorough and avoids later contamination and pipeline blockage; more preferably, the dispenser also includes a liquid separator valve 205 and a liquid outlet branch pipe 206, wherein a plurality of liquid outlet branch pipes 206 are provided at one end of the liquid outlet pipe 204, and a nozzle 207 is installed at the end of the liquid outlet branch pipe 206, wherein the nozzle 207 is a splash-proof nozzle, which can also be provided with a variety of spray modes, such as a shower mode and a booster mode, which is beneficial to select different spray modes according to culture bottles and culture dishes of different sizes to avoid splashing of the culture medium during packaging.

[0033] The liquid separation valve 205 is arranged at the connection between the liquid outlet pipe 204 and the liquid outlet branch pipe 206, and is used to control the opening and closing of the liquid outlet branch pipe. It can quantitatively package multiple culture media at the same time, greatly shorten the culture medium packaging time, and help improve efficiency.

[0034] Furthermore, the integrated device also includes a stirrer 5, which is arranged in the first cylinder and below the second cylinder, and is used to stir the culture medium in the first cylinder, wherein the bottom of the second cylinder is also provided with a circular hole for the stirrer to pass through and be fixed on the body.

[0035] In some embodiments, the agitator 5 includes an axle 501, a purlin 502, a bearing 503, a motor 504 and an impeller 505, wherein the purlin is connected to the bearing to form an outer ring, and the diameter of the outer ring matches the diameter of the first cylinder, which is used to fix the agitator on the machine body. Specifically, the second cylinder and the first cylinder have corresponding circular holes at the bottom, and the diameter of the circular holes matches the diameter of the bearing, so that the bearing 503 is connected to the outer ring through the circular holes at the bottom of the first cylinder and the second cylinder, and the agitator is fixed on the machine body.

[0036] A motor and an impeller are provided at the bottom end of the bearing 503. The motor is connected to a controller via a wire, and the controller is used to control the motor, turn on or off the stirrer, and control the stirring speed of the stirrer.

[0037] Preferably, the integrated device further comprises a cover for preventing the solid insoluble matter in the second cylinder from overflowing, especially preventing the overflowing during the boiling process, and preventing particles such as impurities in the environment from falling into the culture medium and causing contamination.

[0038] The controller is provided with a control panel for controlling the working status of the solenoid valve, peristaltic pump, dispenser heating wire, body heating wire and stirrer motor, and realizing manual or automatic control of the solenoid valve start and stop, peristaltic pump infusion, dispenser insulation, body heating and stirrer stirring functions.

[0039] The following are examples

[0040] Example 1: Device for integrating preparation and quantitative packaging of microbial culture medium

[0041] The device, such as Figure 1 As shown, it can realize the standardized production and quantitative packaging of microbial culture medium, and specifically includes a base 1, a liquid dispenser 2, a controller 3, a body 4, a stirrer 5 and a cover (not shown).

[0042] Wherein the liquid distributor 2, such as Figure 2 As shown, it is used for quantitative subpackaging of culture medium, and includes a solenoid valve 201, a liquid inlet pipe 202, a peristaltic pump 203, a liquid outlet pipe 204, a liquid separation valve 205, a liquid outlet branch pipe 206 and a nozzle 207, wherein the upper end of the liquid inlet pipe 202 is connected to the solenoid valve 201, and the lower end is connected to the liquid outlet pipe 204 through the peristaltic pump 203, and the liquid outlet pipe 204 is connected to three liquid outlet branch pipes 206 through a four-way liquid separation valve 205, and the nozzle 207 can prevent the liquid culture medium from splashing around when subpackaging, and is installed at the end of the liquid outlet branch pipe 206, and the liquid separation valve 205 is used to control the opening and closing of the liquid outlet branch pipe. The liquid inlet pipe 202, the liquid outlet pipe 204, the liquid outlet branch pipe 206 and the nozzle 207 are all provided with electric heating wires for heat preservation during the subpackaging process of the culture medium. The solenoid valve, the peristaltic pump and the electric heating wire are all controlled by the controller, and each nozzle has an independent liquid separation valve.

[0043] Body 4 Figure 3 As shown, it is arranged on a base, and specifically includes a first hollow cylinder 406 with a bottom and a second cylinder 404, wherein the second cylinder is arranged in the first cylinder, and its diameter and height are smaller than the first cylinder, and the first cylinder is detachably connected to the second cylinder.

[0044] The first cylinder is a stainless steel cylinder with a heat preservation function, which is used to make and load culture medium. A heating wire (controlled by a controller), a liquid outlet 401 and a circular stainless steel filter screen 402 (the screen has 50 meshes and its diameter matches that of the first cylinder) are provided at the bottom thereof, and a liquid level display 403 is provided on the inner wall. The liquid outlet at the bottom of the first cylinder is connected to the liquid inlet pipe of the dispenser through an electromagnetic valve.

[0045] The second cylinder is a stainless steel cylinder with holes, which is used to load solid insoluble substances and / or coagulants when making culture medium. Filter holes (filter hole size is 10 mesh) are distributed on the inner and outer walls and the bottom. A mechanical buckle such as a snap is provided on the top to fix the second cylinder on the first cylinder. A circular hole of the same diameter is provided at the bottom at a corresponding position to the first cylinder for the agitator bearing to pass through.

[0046] Agitator 5 Figure 4 As shown, it is in the shape of an axle, arranged in the first cylinder, and the stirring part (impeller) is located below the second cylinder. The stirrer specifically includes an axle 501, a purlin 502, a bearing 503, a motor 504 and an impeller 505, wherein the purlin is connected to the bearing to form an outer ring, the diameter of the outer ring matches the diameter of the first cylinder, and is used to fix the stirrer on the first cylinder (the bearing needs to pass through the circular holes at the bottom of the first cylinder and the second cylinder); the bottom end of the bearing is connected to the motor and the impeller, and the motor is connected to the controller via a wire.

[0047] The controller 3 is provided with a control panel installed on the outer wall of the base, which is used to control the working status of the solenoid valve, peristaltic pump, dispenser heating wire, body heating wire and agitator motor, and can realize manual and automatic control of the solenoid valve start and stop, peristaltic pump infusion, dispenser insulation, body heating and agitator stirring functions.

[0048] The machine cover (not shown) is fixed or detachable, matched with the first cylinder, and used to seal the machine body to prevent the solid insoluble matter in the second cylinder from overflowing during the boiling process.

[0049] Example 2: Device for integrating preparation and quantitative packaging of microbial culture medium

[0050] The device comprises a base 1, a liquid dispenser 2, a controller 3 and a body 4;

[0051] The controller 3 is provided with a control panel, which is arranged on the side of the base 1 and is used to control the heating of the machine body and the quantitative dispensing of the dispenser;

[0052] The liquid dispenser 2 is used for quantitatively dispensing culture medium, and comprises a solenoid valve 201, a liquid inlet pipe 202, a peristaltic pump 203, a liquid outlet pipe 204 and a nozzle 207, wherein the upper end of the liquid inlet pipe 202 is connected to the solenoid valve 201, and the lower end is connected to the liquid outlet pipe 204 through the peristaltic pump 203, and the end of the liquid outlet pipe 204 is connected to the nozzle 207, and the nozzle 207 can prevent the liquid culture medium from splashing around when dispensing. The solenoid valve 201 is used to quantitatively control the outflow of the culture medium. The liquid inlet pipe 202 and the liquid outlet pipe 204 are both provided with electric heating wires for heat preservation during the dispensing process of the culture medium. The solenoid valve, the peristaltic pump and the electric heating wire are all controlled by the controller.

[0053] The body 4 is arranged on the base, and specifically comprises a first hollow cylinder with a bottom and a second cylinder, wherein the second cylinder is arranged in the first cylinder, and its diameter and height are smaller than those of the first cylinder.

[0054] The first cylinder is a stainless steel cylinder with a heat preservation function, which is used to make and load culture medium. A heating wire (controlled by a controller) and a liquid outlet 401 are provided at the bottom of the cylinder. The liquid outlet at the bottom of the first cylinder is connected to the liquid inlet pipe of the dispenser through a solenoid valve.

[0055] The second cylinder is a stainless steel cylinder with filter holes, which is used to load solid insoluble matter when making culture medium. The filter holes are distributed on the inner and outer walls and the bottom, and a heating wire can also be arranged on the bottom.

[0056] Example 3 Standard preparation and quantitative packaging of microbial culture medium

[0057] The integrated device in Example 1 was used to carry out standard preparation and quantitative packaging of culture medium, as follows:

[0058] The solenoid valve in the dispenser is closed by the controller to cut off the passage of the liquid in the machine body to the dispenser. The cleaned stainless steel screen is placed at the bottom of the pot of the first cylinder to prevent the solid residue in the solution from entering the dispenser and causing the pipe to be blocked, and to avoid affecting the final properties of the culture medium. The wheel-shaped agitator is covered on the pot mouth of the machine body, and the wire is connected to the controller. The separate wheel-shaped agitator cooperates with the heat preservation function of the machine body (such as heat preservation of 55℃~65℃), which can accelerate the dissolution of reagents or the precipitation of nutrients, avoid sticking to the pot and agar agglomeration, and can keep the culture solution in a flowing liquid state and uniform nutrition before packaging.

[0059] Add 80% of the required volume of culture medium into the machine body, put the soluble components of the culture medium (such as glucose, peptone) weighed in proportion except agar into the first cylinder or the second cylinder, put the insoluble components (such as peeled potato pieces, sawdust) into the second cylinder, and close the cover to prevent solid particles from overflowing.

[0060] Press the boiling water button on the controller to heat the water in the machine to a slight boil; press the stirring button on the controller to make the stirrer stir the liquid in the machine, adjust the stirring rate to accelerate the dissolution of reagents or the precipitation of nutrients, and avoid sticking the pot. Keep the boiling water button on the controller turned on and maintain the required time for slight boiling until all soluble components of the culture medium are completely dissolved or insoluble solid nutrients are fully precipitated.

[0061] Take out the second cylinder containing the insoluble solids, and add the weighed agar powder to the first cylinder in proportion (if agar powder is not added to the culture medium, this operation is not necessary). Keep the boiling water button on the controller turned on, and increase the stirring speed of the stirrer until the liquid in the first cylindrical pot is uniform and transparent. Add distilled water to the first cylindrical pot until the liquid level indicator shows the required volume of culture medium.

[0062] Keep the boiling button on the controller turned on and the stirring speed of the stirrer turned on, and boil the culture solution in the first cylindrical pot again until the liquid in the first cylindrical pot is uniform and transparent, then turn off the boiling button. Turn on the insulation button on the controller to maintain the temperature of the culture solution in the first cylindrical pot at 55℃~65℃, slow down the stirring speed of the stirrer, and maintain the slow stirring state until the culture solution is packaged. The specific packaging is as follows:

[0063] The controller opens the electromagnetic valve in the dispenser to open the channel for the liquid in the first cylindrical pot to flow to the dispenser. The controller controls the power and start / stop state of the peristaltic pump to provide continuous outflow power for the viscous culture fluid. The peristaltic pump and its upstream and downstream heated and insulated (55°C to 65°C) liquid pipes can maintain the liquid state of the agar culture medium to avoid pipe blockage.

[0064] The controller turns on the insulation switch of the dispenser to maintain the internal liquid temperature of the liquid inlet pipe and the liquid outlet pipe at 55°C to 65°C.

[0065] Open the corresponding valve on the four-way liquid dispensing valve of the liquid outlet pipe according to the number of required infusion pipes, adjust the output power and start time through the controller, or directly set the volume of liquid flowing out of the corresponding nozzle. Open the nozzle valve, use a triangular flask or test tube to collect the quantitative liquid culture medium flowing out under the nozzle of the liquid outlet branch pipe, and sterilize it in time or use it for other purposes.

[0066] After the culture medium in the machine is packaged, add tap water to the machine, boil it, clean the pot and pipes, and turn off all buttons.

[0067] The parameters set by the above controller (boiling time, holding time, stirring rate, peristalsis time, etc.) correspond to the type and state of the prepared culture medium, and can be directly used when the culture medium is prepared again after being stored in the control system. Add all kinds of reagents except agar in advance, press the quick start button, and only need to add agar or replenish distilled water in the middle according to the prompts of the control panel to achieve standardization of the preparation of different batches of culture medium and reduce the differences between batches, especially nutrient imbalance.

[0068] The above description is only a preferred embodiment of the present invention and does not constitute any form of limitation to the present invention. Although the present invention has been disclosed as a preferred embodiment as above, it is not intended to limit the present invention. Any technical personnel in this field can make some changes or modify the technical contents disclosed above into equivalent embodiments without departing from the scope of the technical solution of the present invention. However, any brief modifications, equivalent changes and modifications made to the above embodiments based on the technical essence of the present invention without departing from the content of the technical solution of the present invention are still within the scope of the technical solution of the present invention.

Claims

1. A device for integrating the preparation and quantitative packaging of microbial culture medium, characterized in that: The device comprises a base (1), a liquid dispenser (2), a controller (3) and a body (4); wherein the liquid dispenser (2) is used for quantitatively dispensing a culture medium; and the body (4) is arranged on the base (1) and connected to the liquid dispenser (2); The body (4) comprises a first hollow cylinder with a bottom and a second cylinder, wherein the second cylinder is arranged in the first cylinder, and its diameter and height are both smaller than the first cylinder; the first cylinder is used for making and loading culture medium, and has a heating wire and a liquid outlet (401) at its bottom; The second cylinder is used to load solid insoluble matter when preparing culture medium, and filtering holes are distributed on the inner and outer walls and bottom of the second cylinder.

2. The device for integrating microbial culture medium preparation and quantitative packaging as claimed in claim 1, characterized in that: The first cylinder is also provided with a stainless steel filter screen at its bottom, the mesh of which is smaller than the filter holes of the second cylinder, for secondary filtration.

3. The device for integrating microbial culture medium preparation and quantitative packaging as claimed in claim 2, characterized in that: The first cylinder and the second cylinder are detachably connected; the stainless steel filter screen is circular, and its diameter matches that of the first cylinder, and is detachably installed.

4. The device for integrating microbial culture medium preparation and quantitative packaging as claimed in claim 3, characterized in that: The first cylinder has a liquid level indicator (403) on its inner wall; the second cylinder has a mechanical buckle on its top end for fixing on the first cylinder.

5. The device for integrating the preparation and quantitative packaging of microbial culture medium according to any one of claims 1 to 4, characterized in that: The liquid dispenser (2) has a liquid inlet end arranged inside the base and a liquid outlet end arranged on the side of the base, and specifically comprises a solenoid valve (201), a liquid inlet pipe (202), a peristaltic pump (203), a liquid outlet pipe (204) and a nozzle (207), wherein the upper end of the liquid inlet pipe (202) is connected to the solenoid valve (201), and the lower end is connected to the liquid outlet pipe (204) via the peristaltic pump (203), and the nozzle (207) is provided at the end of the liquid outlet pipe (204).

6. The device for integrating microbial culture medium preparation and quantitative packaging as claimed in claim 5, characterized in that: The liquid inlet pipe (202) and / or the liquid outlet pipe (204) are provided with electric heating wires for heat preservation during the culture medium packaging process.

7. The device for integrating microbial culture medium preparation and quantitative packaging according to claim 6, characterized in that: The liquid distributor (2) further comprises a liquid distributor valve (205) and a liquid outlet branch pipe (206), wherein one end of the liquid outlet pipe (204) is connected to a plurality of liquid outlet branch pipes (206) via a multi-way liquid distributor valve, a nozzle (207) is installed at the end of the liquid outlet branch pipe (206), and the liquid distributor valve (205) is used to control the opening and closing of the liquid outlet branch pipe.

8. The device for integrating microbial culture medium preparation and quantitative packaging as claimed in claim 7, characterized in that: The device also includes a stirrer (5) which is arranged in the first cylinder.

9. The device for integrating microbial culture medium preparation and quantitative packaging as claimed in claim 8, characterized in that: The stirring part of the stirrer (5) is located below the second cylinder, and comprises an axle (501), a purlin (502), a bearing (503), a motor (504) and an impeller (505), wherein the purlin is connected to the bearing to form an outer ring, the diameter of the outer ring matches the diameter of the first cylinder, and is used to fix the stirrer on the first cylinder.

10. The device for integrating microbial culture medium preparation and quantitative packaging according to claim 9, characterized in that: The device also includes a cover for preventing the solid insoluble matter in the second cylinder from overflowing.

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