Feeding device for valine anaerobic fermentation

Abstract

The utility model discloses a feeding device for valine anaerobic fermentation, and belongs to the technical field of anaerobic fermentation. The liquid ammonia mixing device solves the problems that the volume utilization rate of fermentation equipment is low and the cost is high when liquid ammonia is mixed in the existing fed-batch anaerobic fermentation. The liquid ammonia buffer tank is communicated with the sugar-ammonia mixing tank through a liquid ammonia output pipe, a first proportioning valve is arranged on the liquid ammonia output pipe, a second proportioning valve is arranged on the liquid sugar input pipe, and the compressor is communicated between the top of the sugar-ammonia mixing tank and the top of the liquid buffer tank through a pipeline. The absorption tank is communicated with the top of the sugar-ammonia mixing tank through a pressure relief pipe. Supplementary sugar and liquid ammonia in the fermentation process are mixed in advance, and the opening degrees of the first proportioning valve and the second proportioning valve in the material supplementing device are fed back and adjusted according to the pH change of a culture medium in the fermentation process, so that the pH stability in the fermentation process is ensured, the liquid ammonia supplementation is maintained, and the volume utilization rate of a fermentation tank and the process fermentation intensity are ensured; the extraction process cost after fermentation is reduced.

Description

Technical Field

[0001] The utility model relates to a feeding device for valine anaerobic fermentation, belonging to the technical field of anaerobic fermentation. Background Art

[0002] The current mainstream fermentation process for valine adopts anaerobic fermentation technology. During the fermentation process of valine, sterilized glucose and liquid ammonia need to be added through pipelines as the carbon source and nitrogen source required for the normal metabolism of the bacteria, respectively. This method uses the kinetic energy of compressed air in the process to connect the liquid ammonia pipeline to the sterile air pipeline, and diffuses the liquid ammonia evenly into the fermentation liquid through the diffusion of air during fermentation, thereby supplementing the inorganic nitrogen source required for bacterial anabolism and adjusting the pH value of the fermentation liquid. However, in anaerobic fermentation, if sterile air is introduced during the fermentation process of valine, the oxygen concentration in the fermentation liquid will increase. The oxygen in the sterile air will change the metabolic pathway of the bacteria, resulting in a decrease in the valine synthesis flux, which not only increases the energy cost of the input fermentation tank, but also changes the metabolic pathway of the bacteria, resulting in a decrease in fermentation indicators.

[0003] Therefore, in this fed-batch fermentation mode, how to ensure the uniform diffusion of liquid ammonia in the fermentation liquid without ventilation becomes a key factor affecting the fermentation energy consumption and indicators under this mode;

[0004] In order to ensure the uniform diffusion of liquid ammonia, a common method is to use ammonia water to supplement liquid ammonia during fermentation. However, there are several problems in using ammonia water to diffuse ammonia in the hair spray tank: first, the preparation of ammonia water requires the purchase of additional equipment, which increases the cost of ammonia water preparation and storage; second, the ammonia content of ammonia water is low, and a large amount of water is added during the process of supplementing ammonia water, resulting in a decrease in the concentration of the fermentation medium, affecting the volume utilization rate of the fermentation equipment, and then causing the volume of the fermentation tank to be used up in advance, resulting in the early end of fermentation; third, the addition of ammonia water causes the fermentation liquid to be diluted, and the product concentration is low. The subsequent extraction process needs to evaporate the water brought in by the ammonia water during the fermentation, which will increase the cost and process of the subsequent extraction process, and have a more obvious negative impact on the fermentation production line. In addition, the output of the entire positive production line will decrease, and the equipment maintenance cycle will be shortened, among other problems. Utility Model Content

[0005] The utility model is to solve the above technical problems and further provides a feeding device for anaerobic fermentation of valine.

[0006] The technical solution adopted by the utility model to solve the above technical problems is:

[0007] A feeding device for anaerobic fermentation of valine comprises a liquid ammonia buffer tank, a sugar-ammonia mixing tank, an absorption tank and a compressor, wherein the liquid ammonia buffer tank is connected to the sugar-ammonia mixing tank through a liquid ammonia output pipe, the sugar-ammonia mixing tank is also connected to a liquid sugar input pipe, the liquid ammonia output pipe is provided with a first proportional regulating valve, the liquid sugar input pipe is provided with a second proportional regulating valve, the compressor is connected and arranged between the top of the sugar-ammonia mixing tank and the top of the liquid tank buffer tank through a pipeline, the absorption tank is connected to the top of the sugar-ammonia mixing tank through a pressure relief pipe, and the pressure relief pipe is provided with a first pressure gauge and a pressure relief valve, the upper part of the sugar-ammonia mixing tank is connected and provided with a mixed liquid output pipe, and the mixed liquid output pipe is provided with a third proportional regulating valve.

[0008] Furthermore, a liquid level sensor is provided in the sugar-ammonia mixing tank.

[0009] Furthermore, the liquid ammonia buffer tank is also connected to a liquid ammonia input pipe, and a fourth proportional regulating valve is provided on the liquid ammonia input pipe.

[0010] Furthermore, a check valve is provided on the liquid sugar input pipe.

[0011] Furthermore, a first flow rate sensor is also provided on the liquid ammonia output pipe, and the first flow rate sensor is connected to the first proportional regulating valve signal.

[0012] Furthermore, a second flow rate sensor is provided on the liquid sugar input pipe, and the second flow rate sensor is connected to the second proportional regulating valve signal.

[0013] Furthermore, a third flow rate sensor is also provided on the mixed liquid output pipe, and the third flow rate sensor is connected to the third proportional regulating valve signal.

[0014] Furthermore, a second pressure gauge is connected to the liquid ammonia buffer tank.

[0015] Furthermore, a third pressure gauge is connected to the liquid ammonia input pipe.

[0016] Furthermore, the liquid ammonia output pipe and the liquid sugar input pipe are both connected to the lower part of the sugar-ammonia mixing tank.

[0017] Compared with the prior art, the utility model has the following effects:

[0018] By means of the feeding device of the utility model, the sugar supplement and liquid ammonia are pre-mixed during the fermentation process, and according to the pH change of the culture medium during the fermentation process, the openings of the first proportional regulating valve and the second proportional regulating valve in the feeding device are feedback-regulated to ensure that the pH of the fermentation process is stable while maintaining the replenishment of liquid ammonia, while ensuring the volume utilization rate of the fermentation tank and the fermentation intensity of the process, reducing the cost of the post-fermentation extraction process, and effectively solving the problem of liquid ammonia mixing in batch fed anaerobic fermentation in the prior art. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 This is a schematic diagram of the structure of this application.

[0020] In the figure:

[0021] 1. Liquid ammonia buffer tank; 2. Sugar ammonia mixing tank; 3. Absorption tank; 4. Compressor; 5. First proportional regulating valve; 6. Second proportional regulating valve; 7. First pressure gauge; 8. Pressure relief valve; 9. Third proportional regulating valve; 10. Liquid level sensor; 11. Fourth proportional regulating valve; 12. Check valve; 13. First flow rate sensor; 14. Second flow rate sensor; 15. Third flow rate sensor; 16. Second pressure gauge; 17. Third pressure gauge. DETAILED DESCRIPTION

[0022] Specific implementation method 1: Combination Figure 1 This implementation mode is explained by clearly and completely describing the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the utility model.

[0023] It should be noted that the descriptions of the present invention regarding directions such as "left", "right", "left side", "right side", "upper", "lower", "top", "bottom", etc. are all defined based on the relationship between the orientations or positions shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the structure described must be constructed and operated in a specific orientation. Therefore, it cannot be understood as a limitation on the present invention. In the description of the present invention, the meaning of "multiple" is more than two, unless otherwise clearly and specifically defined.

[0024] In the description of the present invention, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a direct connection, or an indirect connection through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0025] A feeding device for anaerobic fermentation of valine comprises a liquid ammonia buffer tank 1, a sugar-ammonia mixing tank 2, an absorption tank 3 and a compressor 4, wherein the liquid ammonia buffer tank 1 is connected to the sugar-ammonia mixing tank 2 through a liquid ammonia output pipe, the sugar-ammonia mixing tank 2 is also connected to a liquid sugar input pipe, the liquid ammonia output pipe is provided with a first proportional regulating valve 5, the liquid sugar input pipe is provided with a second proportional regulating valve 6, the compressor 4 is arranged between the top of the sugar-ammonia mixing tank 2 and the top of the liquid tank buffer tank through a pipeline connection, the absorption tank 3 is connected to the top of the sugar-ammonia mixing tank 2 through a pressure relief pipe, and the pressure relief pipe is provided with a first pressure gauge 7 and a pressure relief valve 8, the upper part of the sugar-ammonia mixing tank 2 is connected with a mixed liquid output pipe, and the mixed liquid output pipe is provided with a third proportional regulating valve 9.

[0026] The flow rate of liquid ammonia entering the sugar-ammonia mixing tank 2 is adjusted by the first proportional regulating valve 5;

[0027] The flow rate of the liquid sugar entering the sugar-ammonia mixing tank 2 is adjusted by the second proportional regulating valve 6, thereby controlling the sugar replenishment rate entering the fermentation tank.

[0028] When the fermentation pH is high, the compressor 4 re-condenses and compresses the ammonia gas in the sugar-ammonia mixing tank 2 through compression and cooling, thereby reducing the ammonia pressure in the sugar-ammonia mixing tank 2 to adjust the ammonia partial pressure in the sugar-ammonia mixing tank 2 and reduce the solubility of ammonia in sugar. According to the pH feedback from the fermentation tank, the device controls the mixing ratio of sugar and liquid ammonia and recovers part of the ammonia through the compressor 4 for adjustment, so that the pH of the fermentation tank is constant within the range of 7.0 to 7.5.

[0029] When the first proportional regulating valve 5 fails, excessive liquid ammonia enters the sugar-ammonia mixing tank 2, causing overpressure in the sugar-ammonia mixing tank 2. By arranging a first pressure gauge 7 and a pressure relief valve 8 on the pressure relief pipe, when the first pressure gauge 7 detects overpressure in the sugar-ammonia mixing tank 2, the pressure relief valve 8 opens, and the ammonia gas is absorbed and depressurized by the water in the absorption tank 3.

[0030] The first proportional regulating valve 5, the second proportional regulating valve 6, the third proportional regulating valve 9 and the compressor 4 are all interlocked with the online pH monitoring meter of the fermentation tank to participate in the pH regulation of the fermentation process. The opening of each proportional regulating valve and the power of the compressor 4 are adjusted according to the fermentation pH feedback.

[0031] The liquid ammonia output pipe is connected to the lower part of the liquid ammonia buffer tank 1 .

[0032] By means of the feeding device of the utility model, the sugar supplement and liquid ammonia are pre-mixed during the fermentation process, and according to the pH change of the culture medium during the fermentation process, the openings of the first proportional regulating valve 5 and the second proportional regulating valve 6 in the feeding device are feedback-regulated to ensure that the pH of the fermentation process is stable while maintaining the replenishment of liquid ammonia, while ensuring the volume utilization rate of the fermentation tank and the fermentation intensity of the process, reducing the cost of the post-fermentation extraction process, and effectively solving the problem of liquid ammonia mixing in batch fed anaerobic fermentation in the prior art.

[0033] A liquid level sensor 10 is provided in the sugar-ammonia mixing tank 2. Designed in this way, the liquid level sensor 10 is connected to the first proportional regulating valve 5, the second proportional regulating valve 6 and the third proportional regulating valve 9 by signals, and is used to feedback control the opening of the first proportional regulating valve 5 and the second proportional regulating valve 6, as well as the flow rate into the fermentation tank.

[0034] The liquid ammonia buffer tank 1 is also connected to a liquid ammonia input pipe, and a fourth proportional regulating valve 11 is arranged on the liquid ammonia input pipe. In this design, the fourth proportional regulating valve 11 controls the liquid ammonia to enter the liquid ammonia buffer tank 1. The liquid ammonia input pipe is connected to the lower part of the liquid ammonia buffer tank 1.

[0035] The liquid sugar input pipe is provided with a check valve 12. Such a design prevents the sugar-ammonia mixing tank 2 from flowing back into the sugar storage tank.

[0036] A first flow rate sensor 13 is also provided on the liquid ammonia output pipe, and the first flow rate sensor 13 is signal-connected to the first proportional control valve 5. In this design, the liquid ammonia flow rate in the liquid ammonia output pipe is fed back by the first flow rate sensor 13, thereby controlling the opening of the first proportional control valve 5.

[0037] The liquid sugar input pipe is also provided with a second flow rate sensor 14, and the second flow rate sensor 14 is signal-connected to the second proportional regulating valve 6. In this design, the second flow rate sensor 14 feeds back the liquid sugar flow rate in the liquid sugar input pipe, thereby controlling the opening of the second proportional regulating valve 6.

[0038] A third flow rate sensor 15 is also provided on the mixed solution output pipe, and the third flow rate sensor 15 is signal-connected to the third proportional regulating valve 9. In this design, the flow rate of the aminoglucose mixed solution in the mixed solution output pipe is fed back by the third flow rate sensor 15, thereby controlling the opening of the third proportional regulating valve 9.

[0039] The liquid ammonia buffer tank 1 is connected with a second pressure gauge 16. In this design, the pressure in the liquid ammonia buffer tank 1 is monitored and fed back by setting the second pressure gauge 16.

[0040] The liquid ammonia inlet pipe is connected with a third pressure gauge 17. In this design, the pressure in the liquid ammonia inlet pipe is monitored and fed back by setting the third pressure gauge 17.

[0041] The liquid ammonia output pipe and the liquid sugar input pipe are both connected to the lower part of the sugar-ammonia mixing tank 2 .

[0042] In the anaerobic fermentation of valine, the feeding device for anaerobic fermentation of valine is used to mix liquid ammonia and liquid sugar, and the air volume is saved by 3000m per hour during fermentation. 3 / h, the fermentation index conversion rate is increased from 59% in the original process to 62%, achieving complete anaerobic fermentation in the process (the conversion rate calculation formula is: acid production * fermentation volume / total sugar. This calculation formula is a prior art and will not be repeated here).

[0043] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes within the technical scope disclosed by the present invention according to the technical scheme and the utility model concept of the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A feeding device for anaerobic fermentation of valine, characterized in that: The invention comprises a liquid ammonia buffer tank (1), a sugar-ammonia mixing tank (2), an absorption tank (3) and a compressor (4), wherein the liquid ammonia buffer tank (1) is connected to the sugar-ammonia mixing tank (2) through a liquid ammonia output pipe, the sugar-ammonia mixing tank (2) is also connected to a liquid sugar input pipe, the liquid ammonia output pipe is provided with a first proportional regulating valve (5), the liquid sugar input pipe is provided with a second proportional regulating valve (6), the compressor (4) is arranged between the top of the sugar-ammonia mixing tank (2) and the top of the liquid tank buffer tank through a pipeline connection, the absorption tank (3) is connected to the top of the sugar-ammonia mixing tank (2) through a pressure relief pipe, the pressure relief pipe is provided with a first pressure gauge (7) and a pressure relief valve (8), the upper part of the sugar-ammonia mixing tank (2) is connected to a mixed liquid output pipe, and the mixed liquid output pipe is provided with a third proportional regulating valve (9).

2. A feeding device for anaerobic fermentation of valine according to claim 1, characterized in that: A liquid level sensor (10) is arranged in the sugar-ammonia mixing tank (2).

3. A feeding device for anaerobic fermentation of valine according to claim 1, characterized in that: The liquid ammonia buffer tank (1) is also connected to a liquid ammonia input pipe, and a fourth proportional regulating valve (11) is provided on the liquid ammonia input pipe.

4. A feeding device for anaerobic fermentation of valine according to claim 1, characterized in that: A check valve (12) is provided on the liquid sugar input pipe.

5. A feeding device for anaerobic fermentation of valine according to claim 1, characterized in that: A first flow rate sensor (13) is also provided on the liquid ammonia output pipe, and the first flow rate sensor (13) is connected to the first proportional regulating valve (5) via a signal.

6. A feeding device for anaerobic fermentation of valine according to claim 3, characterized in that: A second flow rate sensor (14) is also provided on the liquid sugar input pipe, and the second flow rate sensor (14) is connected to the second proportional regulating valve (6) via a signal.

7. A feeding device for anaerobic fermentation of valine according to claim 1, characterized in that: A third flow rate sensor (15) is also provided on the mixed liquid output pipe, and the third flow rate sensor (15) is signal-connected to the third proportional regulating valve (9).

8. A feeding device for anaerobic fermentation of valine according to claim 1, characterized in that: A second pressure gauge (16) is connected to the liquid ammonia buffer tank (1).

9. A feeding device for anaerobic fermentation of valine according to claim 3, characterized in that: A third pressure gauge (17) is connected to the liquid ammonia input pipe.

10. The feeding device for anaerobic fermentation of valine according to claim 1, characterized in that: The liquid ammonia output pipe and the liquid sugar input pipe are both connected to the lower part of the sugar-ammonia mixing tank (2).

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