Primary tissue grinding and filtering integrated device

Abstract

The utility model provides a primary tissue grinding and filtering integrated device which comprises a base, a grinding support and a filtering support are arranged on the base, and the grinding support is located on one side of the filtering support; a plurality of grinding grooves are formed in the grinding support, and grinding mechanisms are arranged in the grinding grooves. A filter tank and a tank to be detected are arranged on the filter bracket, and a filter mechanism is arranged in the filter tank. The primary tissue grinding and filtering integrated device disclosed by the utility model is provided with the grinding mechanism and the filtering mechanism, tissues are ground by the grinding mechanism through the matching of the supporting plate, the sleeve and the grinding rod, then ground samples are poured into the filtering pipe group to be filtered, and the filtering pipe group is designed in a sectional manner, so that the filtering efficiency is greatly improved. An operator can reserve a corresponding sample according to the requirement of the particle size, and then the subsequent centrifugation step can be carried out.

Description

Technical Field

[0001] The utility model belongs to the field of experimental instruments, in particular to a primary tissue grinding and filtering integrated device. Background Art

[0002] In biomedical and cell biology research, primary cells have become an important tool for studying cell behavior, physiological mechanisms, and drug action mechanisms because they retain the original tissue structure and functional characteristics of the organism. However, the process of extracting and isolating cells from primary tissues usually involves multiple tedious steps, including tissue grinding, digestion, filtration, and collection. These steps are not only time-consuming and labor-intensive, but also easily cause damage to cells, affecting cell activity and the accuracy of subsequent experiments.

[0003] In traditional primary cell extraction methods, tissue grinding is usually performed mechanically or enzymatically, but these methods often have problems such as complex operation, low efficiency, and large cell loss. Although mechanical grinding can quickly break up tissue, it is easy to cause mechanical damage to cells, resulting in reduced cell activity. Although enzymatic hydrolysis can better protect cell activity, it is complex to operate and time-consuming, and the type and concentration of enzymes have an important influence on the digestion effect of cells and need to be precisely controlled.

[0004] In the process of filtration and collection, traditional methods usually use multi-layer filters or centrifugal separation. Due to the physical effect during the grinding process, cells tend to aggregate and stick to the wall. Especially when the cell concentration is high, cells are more likely to adhere to the container wall during the transfer process, making collection difficult and causing a large number of cells to be lost. Furthermore, although multi-layer filters can effectively remove impurities, they are prone to clogging, resulting in slower filtration speed and increased cell loss. Although centrifugal separation can effectively separate cells and impurities, the mechanical stress generated during the centrifugation process can easily damage cells and affect cell activity.

[0005] In addition, the selection and operation of the filter also have an important impact on the cell collection efficiency. Traditional filters have a fixed pore size and are difficult to adapt to the requirements of cell purity in different experiments. At the same time, improper operation during the filtration process, such as too fast filtration speed or uneven filtration pressure, may cause cells to clog the filter, further reducing the collection efficiency. Summary of the invention

[0006] In view of this, the utility model aims to overcome the defects in the prior art and proposes a primary tissue grinding and filtering integrated device.

[0007] In order to achieve the above object, the technical solution of the utility model is implemented as follows:

[0008] A primary tissue grinding and filtering device comprises a base, wherein a grinding support and a filtering support are arranged on the base, and the grinding support is located on one side of the filtering support;

[0009] The grinding bracket is provided with a plurality of grinding grooves, and a grinding mechanism is arranged inside the grinding grooves; the filtering bracket is provided with a filtering groove and a groove to be tested, and a filtering mechanism is arranged inside the filtering grooves.

[0010] Furthermore, the grinding mechanism includes a grinding cup, a grinding seat, a grinding rod, a support plate, a sleeve, and a grinding head. The grinding cup is placed in the grinding groove. The sleeve is fixed on the support plate and sleeved on the outside of the grinding rod. The top end of the grinding rod is connected to the grinding head, and the bottom end is detachably connected to the grinding seat. The grinding seat is placed in the grinding cup. The bottom of the grinding seat is provided with a plurality of grinding teeth. A pad is provided on the inner side of the grinding cup, and the grinding teeth are located above the pad. The grinding rod and the sleeve are movably connected. The grinding rod can move up and down and rotate in the sleeve, which is convenient for lifting and rotating the grinding seat, thereby grinding the tissue. The pad can reduce the impact of the grinding teeth on the grinding cup and play a buffering role.

[0011] Furthermore, the outer diameter of the support plate is larger than the outer diameter of the grinding cup. The support plate is placed outside the grinding cup, and the outer diameter of the grinding head is larger than the inner diameter of the sleeve, so the support plate is used to limit and support the limit head.

[0012] Furthermore, the filtering mechanism comprises a filtering cover and a filtering tube group, and the filtering cover is threadedly connected to the filtering tube group.

[0013] Furthermore, the filter tube group includes a tertiary filter tube, a secondary filter tube, and a primary filter tube. The top of the primary filter tube is provided with an external thread, and the bottom is provided with an internal thread. The top and bottom of the secondary filter tube are both provided with external threads. The bottom of the tertiary filter tube is a sealing structure. The top of the tertiary filter tube is provided with an internal thread. The filter cover is threadedly connected to the top of the primary filter tube, the top of the secondary filter tube is threadedly connected to the bottom of the primary filter tube, and the bottom of the secondary filter tube is threadedly connected to the top of the tertiary filter tube.

[0014] The aperture of the primary filter screen is larger than that of the secondary filter screen. The filter screen is detachably connected to the fixing groove.

[0015] Furthermore, a primary filter screen and a secondary filter screen are arranged inside the filter tube group, a plurality of fixed grooves are arranged on the inner side of the primary filter tube, the primary filter screen is placed in the fixed groove of the primary filter tube, a fixed groove is arranged on the inner side of the secondary filter tube, and the secondary filter screen is placed in the fixed groove of the secondary filter tube.

[0016] The grinding mechanism adopts a detachable grinding head design made of medical grade stainless steel to ensure that the cells will not be damaged during the grinding process. The grinding seat is equipped with multiple grinding teeth, which can fully break up the primary tissue through rotation and vibration. At the same time, the surface of the grinding seat is coated with an anti-adhesion coating to reduce the phenomenon of cell adhesion during the grinding process.

[0017] The filtration mechanism uses a high-precision filter mesh, and filters of different pore sizes can be replaced according to experimental requirements; the filter tube group adopts a segmented and detachable design, which is convenient for experimenters to collect and process the filtered cell suspension.

[0018] When in use, place the primary tissue into the grinding mechanism, control the lifting and rotation of the grinding seat to break the tissue. The broken tissue fluid enters the filtration mechanism and is filtered through a high-precision filter. Experimenters can adjust the lifting and rotation frequency of the grinding seat as needed, and replace filters with different apertures to meet the needs of different experiments.

[0019] The primary tissue grinding and filtering device integrates the three key steps of grinding, filtering and collecting into one device, while optimizing the operating conditions and parameters of each step, aiming to achieve efficient, rapid and low-loss extraction and separation of primary cells. Specifically, the device adopts a unique grinding structure that can quickly break up tissues while ensuring cell activity; adopts an efficient filtering structure that can quickly and accurately remove impurities while reducing cell loss; and the segmented design of the filter tube group can automatically complete cell collection and reduce the impact of human operation on cells.

[0020] The primary tissue grinding and filtering device greatly improves the extraction and separation efficiency of primary cells, reduces cell loss, and provides a more reliable and efficient experimental tool for biomedical and cell biology research.

[0021] Compared with the prior art, the utility model has the following advantages:

[0022] The primary tissue grinding and filtering device described in the utility model is provided with a grinding mechanism and a filtering mechanism. The tissue is first ground by the grinding mechanism through the cooperation of a support plate, a sleeve and a grinding rod, and then the ground sample is poured into a filter tube group for filtering. The segmented design of the filter tube group allows the operator to retain the corresponding sample according to the particle size requirements, and then the subsequent centrifugation step can be performed. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] The accompanying drawings constituting a part of the present invention are used to provide a further understanding of the present invention. The illustrative embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute an improper limitation on the present invention. In the accompanying drawings:

[0024] Figure 1 This is a schematic diagram of the primary tissue grinding and filtering device according to an embodiment of the utility model;

[0025] Figure 2 This is a schematic diagram of the filtering mechanism (before filtering) described in an embodiment of the utility model;

[0026] Figure 3 It is a schematic diagram of the filtering mechanism (after filtering) described in an embodiment of the utility model.

[0027] Description of reference numerals:

[0028] 1. Base; 2. Grinding bracket; 3. Filter bracket; 4. Grinding mechanism; 5. Filter mechanism; 21. Grinding tank; 31. Filter tank; 32. Test tank; 41. Grinding cup; 42. Grinding teeth; 43. Grinding seat; 44. Grinding rod; 45. Support plate; 46. Sleeve; 47. Grinding head; 48. Pad; 51. Three-stage filter tube; 52. Two-stage filter tube; 53. One-stage filter tube; 54. Filter cover; 55. Fixed tank; 56. One-stage filter screen; 57. Two-stage filter screen. DETAILED DESCRIPTION

[0029] It should be noted that, in the absence of conflict, the embodiments of the present invention and the features in the embodiments can be combined with each other.

[0030] In the description of the present utility model, it should be understood that the terms "center", "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside" and the like indicate positions or positional relationships based on the positions or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present utility model and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present utility model. In addition, the terms "first", "second", etc. are only used for descriptive purposes, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Therefore, features defined as "first", "second", etc. may explicitly or implicitly include one or more of the features. In the description of the present utility model, unless otherwise specified, "multiple" means two or more.

[0031] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installation", "connection" and "connection" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be an indirect connection through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood by specific circumstances.

[0032] The present invention will be described in detail below with reference to the accompanying drawings and in combination with embodiments.

[0033] Example 1

[0034] like Figure 1-3 As shown, a primary tissue grinding and filtering device comprises a base 1, on which a grinding support 2 and a filtering support 3 are arranged, and the grinding support 2 is located on one side of the filtering support 3;

[0035] The grinding bracket 2 is provided with two grinding grooves 21 , and a grinding mechanism 4 is provided inside the grinding grooves 21 ; the filtering bracket 3 is provided with a filtering groove 31 and a testing groove 32 , and a filtering mechanism 5 is provided inside the filtering groove 31 .

[0036] The grinding mechanism 4 includes a grinding cup 41, a grinding seat 43, a grinding rod 44, a support plate 45, a sleeve 46, and a grinding head 47. The grinding cup 41 is placed in the grinding groove 21. The sleeve 46 is fixed on the support plate 45 and sleeved on the outside of the grinding rod 44. The top of the grinding rod 44 is connected to the grinding head 47, and the bottom is detachably connected to the grinding seat 43. The grinding seat 43 is placed in the grinding cup 41. The bottom of the grinding seat 43 is provided with a plurality of grinding teeth 42. The inner side of the grinding cup 41 is provided with a pad 48, and the grinding teeth 42 are located above the pad 48. The grinding rod 44 is movably connected to the sleeve 46. The grinding rod 44 can move up and down and rotate in the sleeve 46, which is convenient for lifting and rotating the grinding seat 43, thereby grinding the tissue. The outer diameter of the support plate 45 is larger than the outer diameter of the grinding cup 41. The support plate 45 is placed outside the grinding cup 41 . The outer diameter of the grinding head 47 is larger than the inner diameter of the sleeve 46 . Therefore, the support plate 45 is used to limit and support the limit head.

[0037] The filtering mechanism 5 includes a filter cover 54 and a filter tube group, and the filter cover 54 is threadedly connected to the filter tube group. The filter tube group is similar in structure and material to a 50mL centrifuge tube. The filter tube group includes a three-stage filter tube 51, a two-stage filter tube 52, and a first-stage filter tube 53. The top of the first-stage filter tube 53 is provided with an external thread, and the bottom is provided with an internal thread. The top and bottom of the second-stage filter tube 52 are both provided with external threads. The bottom of the three-stage filter tube 51 is a sealing structure, and the top of the three-stage filter tube 51 is provided with an internal thread. The filter cover 54 is threadedly connected to the top of the first-stage filter tube 53, the top of the second-stage filter tube 52 is threadedly connected to the bottom of the first-stage filter tube 53, and the bottom of the second-stage filter tube 52 is threadedly connected to the top of the three-stage filter tube 51.

[0038] The filter tube group is provided with a primary filter screen 56 with a pore size of 200 μm and a secondary filter screen 57 with a pore size of 50 μm. The filter screen is detachably connected to the fixed groove 55. Two fixed grooves 55 are provided on the inner side of the primary filter tube 53, and the primary filter screen 56 is placed in the lowest fixed groove 55. The secondary filter tube 52 is provided with a fixed groove 55 on the inner side, and the secondary filter screen 57 is placed in the fixed groove 55.

[0039] Before use, assemble the filter tube group first, install the primary filter screen in the fixing groove 55 at the bottom of the primary filter tube, install the secondary filter screen in the fixing groove 55 of the secondary filter tube, then thread the top of the tertiary filter tube 51 with the bottom of the secondary filter tube 52, thread the top of the secondary filter tube 52 with the bottom of the primary filter tube 53, and finally put them into the filter tank 31, and then put another tertiary filter tube 51 into the test tank 32;

[0040] When in use, the primary tissue solution is first poured into the grinding cup 41 located in the grinding tank 21, and then the grinding seat 43 at the bottom of the grinding rod 44 is placed in the grinding tank 21, so that the grinding teeth 42 contact the pad 48. The operator presses the support plate 45 on the grinding cup 41 with one hand and holds the grinding head 47 with the other hand, and grinds the tissue by lifting and rotating the grinding head 47. After the grinding step is completed, the sample in the grinding cup 41 is poured into the filter tube group, first enters the primary filter tube, and intercepts tissues above 200μm through the primary filter screen, and then enters The tissue on the secondary filter screen 57 is poured into another tertiary filter tube 51 in the test tank 32, and the secondary filter tube 52 is connected to the tertiary filter tube 51 in the test tank 32. Finally, the buffer solution is poured into the tube to flush the tissue remaining on the earphone filter screen into the tertiary filter tube 51, and then connected to the filter cover 54 to perform the subsequent centrifugation step.

[0041] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A primary tissue grinding and filtering integrated device, characterized in that: It comprises a base, on which a grinding bracket and a filtering bracket are arranged, and the grinding bracket is located on one side of the filtering bracket; The grinding bracket is provided with a plurality of grinding grooves, and a grinding mechanism is provided in the grinding grooves; the filtering bracket is provided with a filtering groove and a groove to be tested, and a filtering mechanism is provided inside the filtering groove; The filtering mechanism comprises a filtering cover and a filtering tube set, wherein the filtering cover and the filtering tube set are threadedly connected. The filter tube group includes a three-stage filter tube, a two-stage filter tube, and a first-stage filter tube. The top of the first-stage filter tube is provided with an external thread, and the bottom is provided with an internal thread. The top and bottom of the second-stage filter tube are both provided with external threads. The bottom of the three-stage filter tube is a sealing structure. The top of the three-stage filter tube is provided with an internal thread. The filter cover is threadedly connected to the top of the first-stage filter tube, the top of the second-stage filter tube is threadedly connected to the bottom of the first-stage filter tube, and the bottom of the second-stage filter tube is threadedly connected to the top of the three-stage filter tube.

2. The primary tissue grinding and filtering integrated device according to claim 1, characterized in that: The grinding mechanism includes a grinding cup, a grinding seat, a grinding rod, a support plate, a sleeve, and a grinding head. The grinding cup is placed in the grinding groove. The sleeve is fixed on the support plate and sleeved on the outside of the grinding rod. The top end of the grinding rod is connected to the grinding head, and the bottom end is detachably connected to the grinding seat. The grinding seat is placed in the grinding cup. A plurality of grinding teeth are arranged at the bottom of the grinding seat. A pad is arranged on the inner side of the grinding cup, and the grinding teeth are located above the pad.

3. The primary tissue grinding and filtering integrated device according to claim 2, characterized in that: The outer diameter of the support plate is greater than the outer diameter of the grinding cup.

4. The primary tissue grinding and filtering integrated device according to claim 1, characterized in that: The filter tube group is provided with a primary filter screen and a secondary filter screen inside. The inner side of the primary filter tube is provided with a plurality of fixed grooves. The primary filter screen is placed in the fixed groove of the primary filter tube. The inner side of the secondary filter tube is provided with a fixed groove. The secondary filter screen is placed in the fixed groove of the secondary filter tube.

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