Rotating structure and dissociation kit thereof

Abstract

The utility model relates to the technical field of dissociation kits, and discloses a rotating structure and a dissociation kit thereof, the rotating structure comprises a kit body and a box cover, the kit body comprises an outer box body and an inner box body, one end of the outer box body and one end of the box cover are respectively provided with an opening, and the inner wall of one end, far away from the opening, of the box cover is fixedly connected with an inner cylinder with an annular section; external threads are arranged on the circumferential outer wall of the open end of the outer box body, internal threads are arranged on the circumferential inner wall of the open end of the box cover, the threads of the open end of the outer box body are installed on the circumferential inner side of the open end of the box cover in a matched mode, and the circumferential inner wall of the open end of the outer box body is matched with the circumferential outer wall of the inner cylinder; the box cover is further provided with a dissociation assembly used for tissue dissociation. According to the utility model, the cutting plates which are spirally distributed and obliquely arranged play a role in guiding a cell suspension while cutting a tissue block, so that the cell suspension flows upwards, the sedimentation of a larger cell block is facilitated, and the cell block is fully dissociated.

Description

Technical Field

[0001] The utility model belongs to the technical field of dissociation reagent kits, and in particular relates to a rotating structure and a dissociation reagent kit thereof. Background Art

[0002] In the related art, tissue dissociation is the process of dissociating and digesting tissue blocks into cell clusters or single cells. In order to reduce contamination of tissues during the dissociation process and improve the dissociation efficiency, in the prior art, a rotating mechanism is provided in the reagent kit, and the tissue blocks are cut by a cutting structure provided on the rotating mechanism.

[0003] However, the existing rotary cutting structure usually has a single rotation mode (for example: the tissue dissociation component and device application number disclosed in the prior art: 202211124406.1), and the stirring method of its rotary cutting part is simple, which may lead to poor dispersion of cell tissue in the dissociation solution, thereby possibly affecting the subsequent cell sample adoption effect.

[0004] In view of this, the present utility model is proposed. Utility Model Content

[0005] In order to solve the above technical problems, the basic concept of the technical solution adopted by the utility model is:

[0006] A rotating structure, comprising a reagent box body and a box cover, the reagent box body comprising an outer box body and an inner box body, one end of the outer box body and the box cover are both provided with an opening, the inner wall of the box cover away from the opening is fixedly connected with an inner cylinder with a ring-shaped cross section, the outer wall of the outer box body opening end is provided with an outer thread, the inner wall of the outer box body opening end is provided with an inner thread, the thread of the outer box body opening end is fitted on the inner side of the circumference of the box cover opening end, and the inner wall of the outer box body opening end is matched with the outer wall of the inner cylinder, and the box cover is also provided with a dissociation component for tissue dissociation;

[0007] The dissociation assembly includes a rotating base, a mounting hole is provided at the center position between the inner and outer walls of the end of the box cover away from the opening, and the rotating base is rotatably mounted between the inner walls of the circumference of the mounting hole, a center rod is provided at the center position of the inner end of the rotating base, the center rod is inserted into the reagent kit body at the end away from the rotating base, and a cutting structure for tissue cutting is provided on the outer side of the circumference of the center rod.

[0008] As a preferred embodiment of the present invention, the inner box body is arranged on the inner side of the circumference of the outer box body, and the circumferential outer wall of the inner box body is rotatably matched with the circumferential inner wall of the outer box body.

[0009] As a preferred embodiment of the present utility model, the cutting structure is a cutting plate, which is spirally distributed and fixedly connected to the outer side of the circumference of the central rod, and the cutting plate is arranged in an inclined state.

[0010] As a preferred embodiment of the utility model, a mounting groove is provided at the center of one end of the box cover away from the opening, and an outer end of the rotating base is fixedly connected to a mounting socket in the mounting groove, and the mounting socket and the box cover are rotationally matched.

[0011] As a preferred embodiment of the utility model, a card slot is provided at the center of the inner wall of the inner box body away from the box cover, and a card block is provided at one end of the center rod inserted into the reagent kit body, and the card block is engaged with the inner wall of the card slot.

[0012] As a preferred embodiment of the utility model, guide plates are fixedly connected to the inner walls of the inner box body in a spirally distributed manner, and the guide plates are arranged in an inclined shape.

[0013] As a preferred embodiment of the present invention, the guide plate is arranged outside the circumference of the cutting plate, and the inclination direction of the guide plate is opposite to the inclination direction of the cutting plate.

[0014] A dissociation kit comprises the rotating structure described in any one of the above embodiments.

[0015] Compared with the prior art, the utility model has the following beneficial effects:

[0016] When the device is used, tissue fragments are added to the reagent box body, and then the dissociation component is inserted into the reagent box body, and the box cover is tightly covered on the reagent box body, and the card block is engaged with the card slot. After the preparation is completed, the reagent box is inserted into the dissociation device through the mounting card seat for dissociation treatment. The dissociation device drives the mounting card seat to rotate and causes the central rod to rotate, so that the cutting plate arranged outside the circumference of the central rod dissociates the tissue block. The cutting plate arranged in a spiral distribution and tilting while cutting the tissue block plays a guiding role on the cell suspension, so that the cell suspension flows upward, which is conducive to the sedimentation of larger cell blocks and makes the cell blocks fully dissociated; and the inner box body rotates synchronously with the cooperation of the card block and the card slot, and the guide plate on the inner box body wall opposite to the inclination direction of the cutting plate guides the cell suspension downward, so that the cell suspension flips to produce interactive flow, improves the friction between cell tissues, further improves the tissue dissociation efficiency, and makes the cell suspension more dispersed.

[0017] The specific implementation of the utility model is further described in detail below with reference to the accompanying drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In the attached picture:

[0019] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0020] Figure 2 It is a cross-sectional structural schematic diagram of the utility model;

[0021] Figure 3 It is a schematic diagram of the overall exploded structure of the utility model;

[0022] Figure 4 This is a schematic diagram of the installation structure of the dissociation component of the utility model;

[0023] Figure 5 It is a schematic diagram of the exploded structure of the test kit body of the utility model.

[0024] In the figure: 10, reagent box body; 11, outer box body; 12, inner box body; 13, guide plate; 14, card slot; 20, box cover; 21, inner cylinder; 22, mounting slot; 23, mounting hole; 30, dissociation assembly; 31, rotating base; 32, center rod; 33, cutting plate; 34, card block; 35, mounting card seat. DETAILED DESCRIPTION

[0025] In order to make the purpose, technical scheme and advantages of the embodiments of the utility model clearer, the technical scheme in the embodiments will be clearly and completely described below in conjunction with the drawings in the embodiments of the utility model. The following embodiments are used to illustrate the utility model.

[0026] A rotating structure, such as Figure 1-2 As shown, the reagent box body 10 and the box cover 20 are included. The reagent box body 10 includes an outer box body 11 and an inner box body 12. The outer box body 11 and the box cover 20 are both provided with openings at one end. The inner wall of the box cover 20 away from the opening is fixedly connected with an inner cylinder 21 with a circular cross section. The outer wall of the circumference of the opening end of the outer box body 11 is provided with an external thread, and the inner wall of the circumference of the opening end of the box cover 20 is provided with an internal thread. The thread of the opening end of the outer box body 11 is fitted on the inner side of the circumference of the opening end of the box cover 20, and the inner wall of the circumference of the opening end of the outer box body 11 is matched with the outer wall of the circumference of the inner cylinder 21. The box cover 20 is also provided with a dissociation component 30 for tissue dissociation.

[0027] like Figure 3-4 As shown, the dissociation assembly 30 includes a rotating base 31, a mounting hole 23 is provided at the center position between the inner and outer walls of the end of the box cover 20 away from the opening, and the rotating base 31 is rotatably mounted between the circumferential inner walls of the mounting hole 23, a center rod 32 is provided at the center position of the inner end of the rotating base 31, the center rod 32 is inserted into the reagent kit body 10 at one end away from the rotating base 31, and a cutting structure for tissue cutting is provided on the outer side of the circumference of the center rod 32.

[0028] like Figure 2 As shown, the inner box body 12 is arranged on the inner side of the outer box body 11, and the outer wall of the inner box body 12 is rotatably matched with the inner wall of the outer box body 11.

[0029] like Figure 4As shown, the cutting structure is a cutting plate 33, which is spirally distributed and fixedly connected to the outer side of the circumference of the central rod 32, and the cutting plate 33 is arranged in an inclined state.

[0030] like Figure 4 As shown, a mounting groove 22 is provided at the center of one end of the box cover 20 away from the opening, and an outer end of the rotating base 31 is located in the mounting groove 22 and fixedly connected with a mounting seat 35, and the mounting seat 35 and the box cover 20 are rotationally matched.

[0031] Its function is that when the device is used, tissue fragments are added into the reagent box body 10, and then the dissociation component 30 is inserted into the reagent box body 10, and the box cover 20 is tightly covered on the reagent box body 10, and the card block 34 is engaged with the card slot 14. After the preparation is completed, the reagent box is inserted into the dissociation device through the installation card seat 35 for dissociation treatment. The dissociation device drives the installation card seat 35 to rotate and makes the central rod 32 rotate, so that the cutting plate 33 arranged outside the circumference of the central rod 32 dissociates the tissue block. The cutting plate 33 arranged in a spiral distribution and tilting while cutting the tissue block plays a guiding role on the cell suspension, so that the cell suspension flows upward, which is conducive to the sedimentation of larger cell blocks and makes the cell blocks fully dissociated.

[0032] like Figure 4-5 As shown, a card slot 14 is provided at the center of the inner wall of the inner box body 12 away from the box cover 20 , and a card block 34 is provided at one end of the center rod 32 inserted into the reagent kit body 10 , and the card block 34 is engaged with the inner wall of the card slot 14 .

[0033] like Figure 2 , Figure 5 As shown, guide plates 13 are fixedly connected to the inner wall of the inner box body 12 in a spirally distributed manner, and the guide plates 13 are arranged in an inclined manner.

[0034] like Figure 4-5 As shown, the guide plate 13 is arranged outside the circumference of the cutting plate 33, and the inclination direction of the guide plate 13 is opposite to the inclination direction of the cutting plate 33.

[0035] Its function is that the inner box body 12 rotates synchronously with the cooperation of the block 34 and the slot 14, and the guide plate 13 on the inner wall of the inner box body 12, which is opposite to the inclination direction of the cutting plate 33, guides the cell suspension downward, so that the cell suspension flips to produce interactive flow, increases the friction between cell tissues, further improves the tissue dissociation efficiency, and makes the cell suspension more dispersed.

[0036] like Figure 1-5 As shown, a dissociation kit comprises the rotating structure of any one of the above embodiments.

[0037] It is understood that the present invention is described by some embodiments, and those skilled in the art are aware that various changes or equivalent substitutions may be made to these features and embodiments without departing from the spirit and scope of the present invention. In addition, under the teachings of the present invention, these features and embodiments may be modified to adapt to specific circumstances and materials without departing from the spirit and scope of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed herein, and all embodiments falling within the scope of the claims of this application are within the scope of protection of the present invention.

Claims

1. A rotating structure, comprising a reagent box body (10) and a box cover (20), characterized in that: The reagent box body (10) comprises an outer box body (11) and an inner box body (12), one end of the outer box body (11) and the box cover (20) are both provided with an opening, the inner wall of the box cover (20) away from the opening is fixedly connected with an inner cylinder (21) with a circular cross section, the outer wall of the circumference of the opening end of the outer box body (11) is provided with an external thread, the inner wall of the circumference of the opening end of the box cover (20) is provided with an internal thread, the thread of the opening end of the outer box body (11) is mounted on the inner side of the circumference of the opening end of the box cover (20), and the inner wall of the circumference of the opening end of the outer box body (11) is matched with the outer wall of the circumference of the inner cylinder (21), and the box cover (20) is also provided with a dissociation component (30) for tissue dissociation; The dissociation assembly (30) comprises a rotating base (31), a mounting hole (23) is arranged at a central position between the inner and outer walls of the end of the box cover (20) away from the opening, and the rotating base (31) is rotatably mounted between the circumferential inner walls of the mounting hole (23), a center rod (32) is arranged at a central position of one end of the inner side of the rotating base (31), the end of the center rod (32) away from the rotating base (31) is inserted into the reagent box body (10), and a cutting structure for tissue cutting is arranged on the outer side of the circumference of the center rod (32).

2. The rotating structure according to claim 1, characterized in that: The inner box body (12) is arranged on the inner side of the circumference of the outer box body (11), and the outer wall of the circumference of the inner box body (12) is rotatably matched with the inner wall of the circumference of the outer box body (11).

3. The rotating structure according to claim 1, characterized in that: The cutting structure is a cutting plate (33), which is spirally distributed and fixedly connected to the outer side of the circumference of the central rod (32), and the cutting plate (33) is arranged in an inclined shape.

4. The rotating structure according to claim 1, characterized in that: The box cover (20) is provided with a mounting groove (22) at a central position of one end away from the opening, and an outer end of the rotating base (31) is located in the mounting groove (22) and is fixedly connected with a mounting seat (35), and the mounting seat (35) and the box cover (20) are rotationally matched.

5. The rotating structure according to claim 1, characterized in that: A card slot (14) is provided at the center of the inner wall of one end of the inner box body (12) away from the box cover (20), and a card block (34) is provided at one end of the center rod (32) inserted into the reagent kit body (10), and the card block (34) is engaged with the inner wall of the card slot (14).

6. The rotating structure according to claim 1, characterized in that: A guide plate (13) is fixedly connected and distributed in a spiral manner between the inner walls of the inner box body (12), and the guide plate (13) is arranged in an inclined shape.

7. The rotating structure according to claim 6, characterized in that: The guide plate (13) is arranged outside the circumference of the cutting plate (33), and the inclination direction of the guide plate (13) is opposite to the inclination direction of the cutting plate (33).

8. A dissociation kit, characterized in that: The invention comprises the rotating structure as described in any one of claims 1 to 7.

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