Device for researching dynamic release and function of VOCs (Volatile Organic Compounds) components of soil or microorganisms

Abstract

The utility model discloses a device for studying the dynamic release of VOCs components and the function of soil or microorganisms, which comprises a first air pump and a second air pump, an air flow divider is arranged at the air outlet of the first air pump, and the air flow divider is respectively connected with the air inlet of the second air pump through a processing channel and a contrast channel; a first filter, a first screw gas washing bottle, a first up-down mouth reagent bottle, a first sampler, a first cultivation bottle and a second sampler are sequentially arranged on the treatment channel, the first up-down mouth reagent bottle is used for containing microorganisms or soil, and the first cultivation bottle is used for cultivating plants; a second filter, a second screw gas washing bottle, a second up-down mouth reagent bottle, a second cultivation bottle and a third sampler are sequentially arranged on the contrast channel, the second up-down mouth reagent bottle is an empty bottle, and the second cultivation bottle is used for cultivating plants. The device can monitor and compare plant morphological changes, rhizosphere VOCs component changes and rhizosphere microbiome changes in real time, wherein the plant morphological changes, the rhizosphere VOCs component changes and the rhizosphere microbiome changes are generated by soil or microorganisms.

Description

Technical Field

[0001] The utility model relates to a device for studying the components and functions of VOCs dynamically released by soil or microorganisms. Background Art

[0002] There are a large number of microorganisms in the soil, and these microorganisms carry out metabolic activities such as dynamic material and energy exchange. Due to the complexity and variability of the soil environment, how to understand the dynamic interaction rules of soil microorganisms has become a difficulty in current research. Volatile organic compounds (VOCs) refer to a class of small molecular compounds with a molecular weight of less than 300 Da, low melting point, and high boiling point. As compounds dynamically released during the activities of soil and microorganisms, they can sensitively respond to changes in the structure of microbial communities in the soil. The release of VOCs is affected by many factors such as soil properties, microbial activities, and ambient temperature. Therefore, it is of great scientific significance to study the dynamic process and components of its release. However, at present, most of the focus is on studying the components and functions of VOCs released by soil or microorganisms under static conditions, and the in-situ conditions are limited by factors such as environmental interference, sample contamination, and experimental efficiency, which makes it difficult to observe the dynamic changes of VOCs. Utility Model Content

[0003] The purpose of the utility model is to provide a device for studying the dynamic release of VOCs components and functions from soil or microorganisms, in order to solve the problem that it is difficult to dynamically monitor the changes in the components and functions of VOCs produced by the interaction of soil or microorganisms in real time, so as to achieve real-time monitoring and sampling of soil under simulated in-situ conditions. The device can accurately collect VOCs released from the soil, collect its components and verify their functions, and achieve a comprehensive study of the components and functions of VOCs dynamically released from soil or microorganisms. The device can provide an important tool for the study of soil microbial activity, and help to better understand the dynamic interaction between soil and microorganisms under in-situ conditions.

[0004] The purpose of this utility model is achieved through the following technical solutions:

[0005] A device for studying the dynamic release and function of VOCs from soil or microorganisms comprises a first air pump 1 and a second air pump 3 for dynamically conveying gas, wherein an air flow splitter 2 is arranged at the air outlet of the first air pump 1, and two air outlets of the air flow splitter 2 are respectively connected to the air inlet of the second air pump 3 via a processing channel and a control channel; a first filter 4, a first upper and lower nozzle reagent bottle 6, a first sampler 7, a first cultivation bottle 8, and a second sampler 10 are arranged in sequence on the processing channel, wherein the first upper and lower nozzle reagent bottle 6 is used for holding microorganisms or soil whose VOCs components and functions are to be studied, and the first cultivation bottle 8 is filled with soil or matrix for cultivating plants; a second filter 11, a second upper and lower nozzle reagent bottle 13, a second cultivation bottle 14, and a third sampler 15 are arranged in sequence on the control channel, wherein the second upper and lower nozzle reagent bottle 13 is an empty bottle and does not hold microorganisms or soil whose VOCs components and functions are to be studied, and the second cultivation bottle 14 is filled with soil or matrix for cultivating plants.

[0006] The first air pump 1 and the second air pump 11 are both push-pull air pumps.

[0007] The first filter 4 and the second filter 11 are both sterile filters with a filter membrane pore size of 0.22 μm, which are used to filter and remove large particulate matter and microorganisms in the air.

[0008] A first screw-mouth washing bottle 5 is provided between the first filter 4 and the first upper and lower nozzle reagent bottle 6. The first screw-mouth washing bottle 5 is used for containing sterile water humidified gas, and the air inlet pipe of the first screw-mouth washing bottle 5 extends below the liquid surface; a second screw-mouth washing bottle 12 is provided between the second filter 11 and the second upper and lower nozzle reagent bottle 13. The second screw-mouth washing bottle 12 is used for containing sterile water humidified gas, and the air inlet pipe of the second screw-mouth washing bottle 12 extends below the liquid surface.

[0009] The air inlet of the first upper and lower nozzle reagent bottle 6 is lower than the air outlet; the air inlet of the second upper and lower nozzle reagent bottle 13 is lower than the air outlet. When studying the dynamic release of VOCs components and functions of microorganisms, solid culture medium and microbial bacterial liquid are added to the first upper and lower nozzle reagent bottle 6. When studying the dynamic release of VOCs components and functions of soil, an appropriate and quantitative amount of soil is placed in the first upper and lower nozzle reagent bottle 6.

[0010] The first cultivation bottle 8 and the second cultivation bottle 14 are both cultivation bottles with left and right openings; the first cultivation bottle 8 and the second cultivation bottle 14 are evenly filled with soil or substrate required for crop growth.

[0011] The first culture bottle 8 is provided with a first sealing film 9 for isolating the soil or substrate from the outside air; the second culture bottle 14 is provided with a second sealing film 15 for isolating the soil or substrate from the outside air.

[0012] The first sealing film 9 and the second sealing film 15 are both made of LLDPE.

[0013] The adsorption material of the first sampler 7 is OV-Super Q filler.

[0014] Preferably, the outer shell of the first sampler 7 is made of glass, OV-Super Q filler is filled in the middle of the sampler as an adsorption material, and glass wool is provided at both ends.

[0015] The adsorption material of the second sampler 10 is OV-Super Q filler.

[0016] Preferably, the outer shell of the second sampler 10 is made of glass, OV-Super Q filler is filled in the middle of the sampler as an adsorption material, and glass wool is provided at both ends.

[0017] The adsorption material of the third sampler 16 is OV-Super Q filler.

[0018] Preferably, the outer shell of the third sampler 16 is made of glass, OV-Super Q filler is filled in the middle of the sampler as an adsorption material, and glass wool is provided at both ends.

[0019] The OV-Super Q filler is OV-Super Q filler, 80-100 mesh.

[0020] The first air pump 1 is connected to the air flow diverter 2 by a transparent silicone hose; the gas diverter 2 is connected to the first filter 4 by a transparent silicone hose, and the two adjacent devices in the processing channel are connected by a transparent silicone hose; the second sampler 10 is connected to the second air pump 3 by a transparent silicone hose; the gas diverter 2 is connected to the second filter 11 by a transparent silicone hose, and the two adjacent devices in the control channel are connected by a transparent silicone hose; the third sampler 16 is connected to the second air pump 3 by a transparent silicone hose.

[0021] The device described in the utility model is used to study the dynamic release of VOCs components and functions of soil or microorganisms. The first air pump 1 pushes air, and the second air pump 3 pulls air. Under the push and pull power of the first air pump 1 and the second air pump 3, the gas is diverted by the air flow diverter 2 and enters the treatment channel and the control channel respectively; in the treatment channel, the gas is filtered by the first filter 4 to remove large particulate matter and microorganisms in the air, and then enters the first screw-mouth washing bottle 5 containing sterile water to humidify the gas, and the filtered gas with appropriate humidity is passed into the first upper and lower nozzle reagent bottle 6 for placing microbial culture medium or soil, and then passed into the first sampler 7 for VOCs, and then passed into the first cultivation bottle 8 to make VOCs reach the root zone of plants, and finally enter the second sampler 10; in the control channel, the gas passes through the second filter 11 and the second screw-mouth washing bottle 12 in sequence to obtain filtered gas with appropriate humidity, and then directly passes into the second cultivation bottle 14 through the empty second upper and lower nozzle reagent bottle 13 to reach the root zone of plants, and finally enters the third sampler 16.

[0022] The utility model uses two closed channels with different treatments to allow the generated VOCs to reach the designated location along the device route. The dynamic push-pull system is used to monitor the changes in VOCs components released by microorganisms in real time. By isolating the plants and the VOCs source in two different cylinders, the VOCs source is always not easily polluted, thereby enabling experiments to study the components and functions of VOCs dynamically released by soil or microorganisms. By observing the differences in plant morphology in the treatment channel and the control channel and by comparing and analyzing the content and differences of specific chemical components of VOCs collected by the three samplers, it is possible to clarify the impact of the VOCs produced by the soil or microorganisms on the plants and the differences in components before and after the impact, which can be used to clarify their specific mechanism of action later.

[0023] Beneficial effects of the utility model:

[0024] The utility model uses a set of two-channel separated devices to monitor and compare in real time the plant morphological changes, rhizosphere VOCs component changes and rhizosphere microbial group changes with or without soil or microorganisms producing VOCs; and provides a closed dynamic push-pull volatilization space for VOCs, so that micro-scale experiments on the dynamic release of VOCs components and functions from soil or microorganisms can be carried out.

[0025] The utility model utilizes a sterile filter to filter air impurities before entering the soil or microbial reagent bottle, thereby ensuring that the gas components collected by the VOCs collector will not be affected by other impurities.

[0026] The utility model uses the upper and lower mouth reagent bottles to hold sterile water before placing soil or microorganism reagent bottles, so as to increase air humidity and ensure that after the gas enters the plant root zone, it will not be dried for a long time and continuously dynamically ventilated to cause adverse effects on the root zone.

[0027] The dynamic push-pull system of the utility model can allow VOCs in the soil or microorganisms to affect the root zone of plants faster, more durable and more efficiently, thereby reflecting the functions of the VOCs more quickly.

[0028] The utility model is provided with VOCs samplers in both channels and before and after entering the plant rhizosphere, so that the control group can be fully utilized to exclude the influence of non-experimental variables. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 This is a schematic diagram of the structure of a device for studying the use of volatile substances of beneficial bacteria to control soil-borne diseases in the utility model.

[0030] Figure 2 for Figure 1 Top view of the .

[0031] In the figure, 1-the first air pump, 2-the air flow diverter, 3-the second air pump, 4-the first filter, 5-the first screw-mouth washing bottle, 6-the first upper and lower nozzle reagent bottle, 7-the first sampler, 8-the first cultivation bottle, 9-the first sealing film, 10-the second sampler, 11-the second filter, 12-the second screw-mouth washing bottle, 13-the second upper and lower nozzle reagent bottle, 14-the second cultivation bottle, 15-the second sealing film, and 16-the third sampler. DETAILED DESCRIPTION

[0032] The technical solution of the present invention is further described below in conjunction with the accompanying drawings and specific implementation plans.

[0033] Example 1

[0034] like Figure 1 and Figure 2As shown, a device for studying the dynamic release of VOCs components and functions of soil or microorganisms comprises a first air pump 1 and a second air pump 3 for dynamically conveying gas, wherein an air flow splitter 2 is arranged at the air outlet of the first air pump 1, and the two air outlets of the air flow splitter 2 are connected to the air inlet of the second air pump 3 via a processing channel and a control channel respectively; a first filter 4, a first screw-mouth washing bottle 5, a first upper and lower nozzle reagent bottle 6, a first sampler 7, a first cultivation bottle 8, and a second sampler 10 are arranged in sequence on the processing channel, wherein the first upper and lower nozzle reagent bottle 6 is used to hold the microorganisms or soil whose VOCs components and functions are to be studied, and the first cultivation bottle 8 is filled with soil or matrix for cultivating plants; a second filter 11, a second screw-mouth washing bottle 12, a second upper and lower nozzle reagent bottle 6 are arranged in sequence on the control channel, Bottle 13, a second cultivation bottle 14, and a third sampler 15. The second upper and lower mouth reagent bottle 13 does not contain microorganisms or soil whose VOCs components and functions need to be studied, and the second cultivation bottle 14 is filled with soil or matrix for cultivating plants; the first air pump 1 and the air flow diverter 2 are connected by a transparent silicone hose; the gas diverter 2 and the first filter 4 are connected by a transparent silicone hose, and the two adjacent devices in the processing channel are connected by a transparent silicone hose; the second sampler 10 and the second air pump 3 are connected by a transparent silicone hose; the gas diverter 2 and the second filter 11 are connected by a transparent silicone hose, and the two adjacent devices in the control channel are connected by a transparent silicone hose; the third sampler 16 and the second air pump 3 are connected by a transparent silicone hose; and each connection is sealed and airtight.

[0035] The first air pump 1 and the second air pump 11 are both push-pull air pumps, specifically a portable atmospheric sampling pump (model: ASP-DC-6).

[0036] The air inlet diameter of the air flow distributor 2 is 8 mm, and the air outlet diameter is 8 mm.

[0037] The first filter 4 and the second filter 11 are both sterile filters with a diameter of 50 mm and a filter membrane pore size of 0.22 μm, and are used to filter and remove large particulate matter and microorganisms in the air.

[0038] The first screw-mouth washing bottle 5 and the second screw-mouth washing bottle 12 are used to hold sterile water, and the air inlet pipes of the first screw-mouth washing bottle 5 and the second screw-mouth washing bottle 12 are both extended into the humidified gas below the liquid surface. The first screw-mouth washing bottle 5 and the second screw-mouth washing bottle 12 are 1000mL screw-mouth washing bottles made of high borosilicate glass.

[0039] The air inlet of the first upper and lower nozzle reagent bottle 6 is lower than the air outlet; the air inlet of the second upper and lower nozzle reagent bottle 13 is lower than the air outlet. The first upper and lower nozzle reagent bottle 6 and the second upper and lower nozzle reagent bottle 13 have the same specifications, which are 1000mL, the inner diameter of the bottle mouth is 34mm, the diameter of the bottle body is 102mm, the total height is 213mm, and the outer diameter of the pagoda is 10mm.

[0040] The first culture bottle 8 and the second culture bottle 14 are both glass culture bottles with a capacity of 500 mL and left and right openings, and the inner diameter of the left and right openings is 9 mm; the first culture bottle 8 is provided with a first sealing film 9 for isolating the soil or matrix from the outside air; the second culture bottle 14 is provided with a second sealing film 15 for isolating the soil or matrix from the outside air.

[0041] The first sealing film 9 and the second sealing film 15 are both PE environmentally friendly wrapping films made of LLDPE.

[0042] The shell of the first sampler 7 is made of glass, and the middle of the sampler is filled with OV-Super Q filler, 80-100 mesh, and both ends are glass wool.

[0043] The outer shell of the second sampler 10 is made of glass, and OV-Super Q filler, 80-100 mesh, is filled in the middle of the sampler, and glass wool is filled at both ends.

[0044] The outer shell of the third sampler 16 is made of glass, and OV-Super Q, 80-100 mesh is filled in the middle of the sampler, and glass wool is filled at both ends.

[0045] The inner diameter of the transparent silicone hose is 6mm and the outer diameter is 9mm.

[0046] During the experiment, the first culture bottle 8 and the second culture bottle 14 were filled with the substrate required for crop growth, and tomato seedlings were planted. Then, a sealing film was covered on the substrate to seal the roots of the tomato seedlings to isolate the substrate from the outside air; sterile water was filled in the first screw-mouth washing bottle 5 and the second screw-mouth washing bottle 12 for humidifying the gas; LB solid culture medium was placed in the first upper and lower nozzle reagent bottle 6 and 20 μL of 1×10 8 CFU / mL of Ralstonia solanacearum liquid; the treated channel was regarded as the experimental group and the control channel as the control group, the morphological changes of the plants were observed and recorded every 5 days, the samples collected by three samplers were collected and replaced with new adsorption material Super Q, and the collected data were compared and analyzed after one month to determine its VOCs components and functions.

[0047] The results show that the data collected by the samplers at the same location at different sampling times are different, indicating that the device can reflect the dynamic changes of VOCs components produced by microorganisms. At the same time, new VOCs that were not found in the first and third samplers were monitored at the second sampler in the later stage of the experiment. At the same time, analysis of the rhizosphere microbial community found that the colony structure changed and the proportion of beneficial bacteria increased. At the same time, the dry weight of the plants in the experimental group increased by 24% compared with the control group, which also reflects that the VOCs dynamically released by the microorganism have no significant function in the early stage, but have the effect of promoting plant immunity and promoting the growth of tomato seedlings in the later stage. This shows that the device of this embodiment can be used to determine its VOCs components and functions.

Claims

1. A device for studying the dynamic release of VOCs components and functions from soil or microorganisms, characterized by: It comprises a first air pump and a second air pump for dynamically conveying gas, wherein an air flow splitter is arranged at the air outlet of the first air pump, and two air outlets of the air flow distributor are respectively connected to the air inlet of the second air pump via a processing channel and a control channel; a first filter, a first upper and lower nozzle reagent bottle, a first sampler, a first cultivation bottle and a second sampler are arranged in sequence on the processing channel, the first upper and lower nozzle reagent bottle is used for holding microorganisms or soil whose VOCs components and functions need to be studied, and the first cultivation bottle is filled with soil or matrix for cultivating plants; a second filter, a second upper and lower nozzle reagent bottle, a second cultivation bottle and a third sampler are arranged in sequence on the control channel, the second upper and lower nozzle reagent bottle is an empty bottle, and the second cultivation bottle is filled with soil or matrix for cultivating plants.

2. The device for studying the dynamic release of VOCs components and functions from soil or microorganisms according to claim 1, characterized in that: The first air pump and the second air pump are both push-pull air pumps.

3. The device for studying the dynamic release of VOCs components and functions from soil or microorganisms according to claim 1, characterized in that: The first filter and the second filter are both sterile filters with a filter membrane pore size of 0.22 μm.

4. The device for studying the dynamic release of VOCs components and functions from soil or microorganisms according to claim 1, characterized in that: A first screw-mouth washing bottle is provided between the first filter and the first upper and lower nozzle reagent bottles, the first screw-mouth washing bottle contains sterile water, and the air inlet pipe of the first screw-mouth washing bottle extends below the liquid surface; a second screw-mouth washing bottle is provided between the second filter and the second upper and lower nozzle reagent bottles, the second screw-mouth washing bottle contains sterile water, and the air inlet pipe of the second screw-mouth washing bottle extends below the liquid surface.

5. The device for studying the dynamic release of VOCs components and functions from soil or microorganisms according to claim 1, characterized in that: The air inlet of the first upper and lower nozzle reagent bottle is lower than the air outlet; the air inlet of the second upper and lower nozzle reagent bottle is lower than the air outlet.

6. The device for studying the dynamic release of VOCs components and functions from soil or microorganisms according to claim 1, characterized in that: The first culture bottle and the second culture bottle are both culture bottles with left and right openings.

7. The device for studying the dynamic release of VOCs components and functions from soil or microorganisms according to claim 1, characterized in that: The first culture bottle is provided with a first sealing film for isolating the soil or substrate from the outside air; the second culture bottle is provided with a second sealing film for isolating the soil or substrate from the outside air.

8. The device for studying the dynamic release of VOCs components and functions from soil or microorganisms according to claim 7, characterized in that: The first sealing film and the second sealing film are both made of LLDPE.

9. The device for studying the dynamic release of VOCs components and functions from soil or microorganisms according to claim 1, characterized in that: The adsorption material of the first sampler is OV-Super Q filler; the adsorption material of the second sampler is OV-Super Q filler; the adsorption material of the third sampler is OV-Super Q filler.

10. The device for studying the dynamic release of VOCs components and functions from soil or microorganisms according to claim 9, characterized in that: The shell of the first sampler is made of glass, and OV-Super Q filler is filled in the middle of the sampler, with glass wool at both ends; the shell of the second sampler is made of glass, and OV-Super Q filler is filled in the middle of the sampler, with glass wool at both ends; the shell of the third sampler is made of glass, and OV-Super Q filler is filled in the middle of the sampler, with glass wool at both ends.

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