Near-infrared fluorescence immunoassay quantitative analyzer

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By using a near-infrared photoelectric converter to capture near-infrared fluorescence in the fluorescence immunoassay, the problem of limited detection signal-to-noise ratio in the prior art is solved, and a significant improvement in detection sensitivity and detection accuracy are achieved.

CN222461469UActive Publication Date: 2025-02-11YANTAI NEW DRUG DEV SHANDONG PROVINCIAL LAB

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Patent Information

Application Number: CN202420723488.X
Authority / Receiving Office: CN · China
Patent Type: Utility models(China)
Current Assignee / Owner
Filing Date: 2024-04-09
Publication Date: 2025-02-11
Estimated Expiration: 2034-04-09

AI Technical Summary

Technical Problem

Existing fluorescence immunoassays have interference in detection signal-to-noise ratio (SNR), resulting in limited detection sensitivity.

Method used

The near-infrared fluorescence immunoquantitative analyzer is used to capture the near-infrared fluorescence emitted by fluorescent substances on the reagent card through a near-infrared photoelectric converter, and utilize its strong penetration and anti-background fluorescence interference ability to improve the detection signal-to-noise ratio.

Benefits of technology

It effectively improves the detection signal-to-noise ratio of the equipment, greatly improves the detection sensitivity, good operation effect, and high detection accuracy.

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Abstract

The utility model relates to the technical field of inspection equipment, in particular to a near-infrared fluorescence immunoassay quantitative analyzer which comprises a box body, an information display system fixed on the surface of the box body, and a control circuit system, a material conveying and fixing system and a near-infrared fluorescence detection system which are fixed in the box body, the near-infrared fluorescence detection system is used for exciting and collecting near-infrared fluorescence emission signals and comprises an infrared light source, a first lens, a spectroscope, a second lens, an optical filter and a near-infrared photoelectric converter; exciting light emitted by the infrared light source reaches the spectroscope through the first lens along the horizontal direction; the spectroscope irradiates exciting light to a window area of the reagent card to excite fluorescent substances in a T strip area and a C strip area on the reagent card, then near-infrared fluorescence in the vertical direction is generated respectively, the near-infrared fluorescence passes through the second lens and then passes through the optical filter to remove interference of the exciting light and background fluorescence, and finally the near-infrared fluorescence is captured by the near-infrared photoelectric converter, so that the operation effect is good, and the detection accuracy is high. The detection precision is high.

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Description

Technical Field

[0001] The utility model relates to the technical field of testing equipment, in particular to a near-infrared fluorescent immunoquantitative analyzer. Background Art

[0002] Fluorescence immunochromatography is a simple and rapid diagnostic method, and is also an important tool for point of care testing (POCT). It is mainly composed of a fluorescence immunoassay analyzer and a matching fluorescence immunochromatography reagent card. The fluorescence immunoassay analyzer collects the fluorescence luminescence intensity of two or more strips on the reagent card and calculates the fluorescence intensity ratio between different strips, and substitutes the ratio result into a pre-set standard fitting curve to obtain the test result of the sample to be tested. Among them, depending on the detection principle, the fluorescence intensity ratio is positively or negatively correlated with the concentration of the analyte. This technology has the advantages of simple operation, low cost, rapid detection, strong specificity, high sensitivity, and good stability. It has been widely used in clinical diagnosis, food safety, drug testing, environmental pollution and other fields.

[0003] Highly sensitive fluorescence detection is the key to determining the performance of fluorescence immunoassay analyzers. The fluorescence detectors of conventional fluorescence immunoassay analyzers are usually visible photoelectric sensors, such as charge-coupled device image sensors (CCDs), and their detection wavelength range is mainly in the visible light region (400nm-650nm). However, blood specimens, nitrocellulose membranes, paper strip backings, etc. are prone to produce background fluorescence near the wavelength of the marker emission light under excitation light, which interferes with the detection signal-to-noise ratio (SNR) to a certain extent, thereby restricting the improvement of detection sensitivity. Utility Model Content

[0004] In view of the above technical problems, the utility model provides a near-infrared fluorescence immunoquantitative analyzer.

[0005] The technical solution of the utility model to solve the above technical problems is as follows:

[0006] A near-infrared fluorescence immunoquantitative analyzer comprises a box body, an information display system fixed on the surface of the box body, and a control circuit system, a material conveying and fixing system, and a near-infrared fluorescence detection system fixed inside the box body; the information display system, the material conveying and fixing system, and the near-infrared fluorescence detection system are all electrically connected to the control circuit system;

[0007] The near-infrared fluorescence detection system is used to excite and collect near-infrared fluorescence emission signals, and the near-infrared fluorescence detection system is located on the upper part of the material conveying and fixing system; the near-infrared fluorescence detection system includes an infrared light source, a first lens, a spectroscope, a second lens, a filter and a near-infrared photoelectric converter; the infrared light source is located on the same horizontal line as the first lens and the spectroscope; the second lens, the spectroscope, the filter and the near-infrared photoelectric converter are arranged in sequence from bottom to top; the light-emitting direction of the infrared light source and the photosensing direction of the near-infrared photoelectric converter are perpendicular to each other and are in the same optical path plane; the spectroscope is arranged in the optical path plane and is located at the intersection of the light-emitting direction and the photosensing direction.

[0008] Furthermore, the centers of the second lens, the beam splitter, the filter and the near-infrared photoelectric converter are located on the same vertical line.

[0009] Furthermore, the beam splitter is arranged at an inclined angle of 45 degrees.

[0010] Furthermore, the infrared light source is one of a halogen tungsten lamp, a light emitting diode or a laser diode; and the excitation wavelength of the infrared light source is 600nm-900nm.

[0011] Furthermore, the filter is a narrow-bandpass filter with a wavelength range of 1000nm-1200nm.

[0012] Furthermore, the near-infrared photoelectric converter is one of an indium gallium arsenide photodiode, a photodiode or a photomultiplier tube; and the detection wavelength range of the near-infrared photoelectric converter is 800nm-1700nm.

[0013] Furthermore, the information display system includes a printer and a touch screen display for displaying operating data information.

[0014] Furthermore, the control circuit system includes a control circuit board, and the near-infrared fluorescence detection system, the material conveying and fixing system, the printer and the touch display screen are electrically connected to the control circuit board.

[0015] Furthermore, the material conveying and fixing system includes a reagent card conveying track, a card slot located on the reagent card conveying track, and a stepping drive motor for driving the card slot to move parallel to the reagent card conveying track.

[0016] Furthermore, a reagent card placement opening that is connected to the reagent card conveying track is provided at the front end of the box body, and the card slot slides in and out through the reagent card placement opening.

[0017] In summary, compared with the prior art, the utility model has the following technical effects:

[0018] (1) In the present invention, the fluorescent immunoassay quantitative analyzer adopts a near-infrared photoelectric converter to replace the visible photoelectric sensor, which can efficiently capture the near-infrared fluorescence emitted by the fluorescent substance on the reagent card; by utilizing the strong penetration and anti-background fluorescence interference ability of the near-infrared fluorescence, the signal-to-noise ratio of the device detection can be effectively improved, thereby greatly improving the detection sensitivity of the device.

[0019] (2) In the utility model, the reagent card reaches and is fixed in the photosensitive area of the near-infrared photoelectric converter along the reagent card conveying track; the excitation light emitted by the infrared light source reaches the spectroscope through the first lens in the horizontal direction; the spectroscope irradiates the excitation light to the window area of the reagent card, thereby stimulating the fluorescent substances specifically aggregated in the T-strip and C-strip areas on the nitrocellulose membrane of the reagent card, and then generates near-infrared fluorescence in the vertical direction respectively. The near-infrared fluorescence passes through the second lens and then through the filter to remove the interference of the excitation light and background fluorescence, and is finally captured by the near-infrared photoelectric converter, with good operation effect and high detection accuracy.

[0020] (3) In the present invention, the near-infrared fluorescence immunoassay quantitative analyzer has a reasonable structural design and low manufacturing cost, and has the characteristics of fast detection speed, low detection signal-to-noise ratio, high detection precision, and good accuracy, and can be widely used in various rapid detection scenarios. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 This is a schematic diagram of the structure of the near-infrared fluorescence detection system of the utility model;

[0022] Figure 2 This is a schematic diagram of the structure of the near-infrared fluorescence immunoquantitative analyzer of the present utility model.

[0023] In the accompanying drawings, the component names represented by the reference numerals are listed as follows:

[0024] 10. Box body; 11. Touch screen; 12. Printer; 13. Control circuit board; 14. Card slot; 15. Reagent card conveying track; 1611. Infrared light source; 1612. First lens; 1613. Spectroscope; 1614. Second lens; 1615. Filter; 1616. Near-infrared photoelectric converter; 17. Reagent card. DETAILED DESCRIPTION

[0025] The principles and features of the present invention are described below in conjunction with the accompanying drawings. The examples given are only used to explain the present invention and are not used to limit the scope of the present invention.

[0026] Reference Figure 1-Figure 2In this embodiment, a near-infrared fluorescence quantitative immunoassay analyzer is disclosed, including a box 10, an information display system fixed on the surface of the box 10, and a control circuit system, a material conveying and fixing system, and a near-infrared fluorescence detection system fixed inside the box 10; the near-infrared fluorescence detection system is located on the upper part of the material conveying and fixing system; the information display system, the material conveying and fixing system, and the near-infrared fluorescence detection system are all electrically connected to the control circuit system; the information display system is used to display operating data and sample test results; the material conveying and fixing system is used to transport the reagent card 17 to the observation position of the near-infrared fluorescence detection system and fix it; the near-infrared fluorescence detection system is used to excite and collect near-infrared fluorescence emission signals; the control circuit system is used to control and adjust each system.

[0027] In this embodiment, the near-infrared fluorescence detection system is used to excite and collect near-infrared fluorescence emission signals, and the near-infrared fluorescence detection system includes an infrared light source 1611, a first lens 1612, a spectroscope 1613, a second lens 1614, a filter 1615 and a near-infrared photoelectric converter 1616; the infrared light source 1611, the first lens 1612 and the spectroscope 1613 are located on the same horizontal line, and the infrared light source 1611 is arranged in parallel with the first lens 1612; the second lens 1614, the spectroscope 1613, the filter 1615 and the near-infrared photoelectric converter 1616 are arranged in sequence from bottom to top, in order to ensure Light can be accurately propagated and detected along a predetermined path. The centers of the second lens 1614, the spectroscope 1613, the filter 1615 and the near-infrared photoelectric converter 1616 are located on the same vertical line, reducing the loss of light during transmission. The light emitting direction of the infrared light source 1611 and the photosensing direction of the near-infrared photoelectric converter 1616 are perpendicular to each other and are in the same optical path plane. The spectroscope 1613 is arranged in the optical path plane and is located at the intersection of the light emitting direction and the photosensing direction. In order to ensure that the excitation light emitted by the infrared light source 1611 irradiates the surface of the spectroscope 1613, the spectroscope 1613 is arranged at an inclined angle of 45 degrees.

[0028] Specifically, the infrared light source 1611 and the first lens 1612 are arranged in the control box, the second lens 1614, the spectroscope 1613, the filter 1615 and the near-infrared photoelectric converter 1616 are arranged on the inner side of the support frame, and the control box is arranged on one side of the detection support frame.

[0029] In a specific embodiment, the infrared light source 1611 is one of a halogen tungsten lamp, a light emitting diode or a laser diode; the excitation wavelength of the infrared light source 1611 is 600nm-900nm. The filter 1615 is a narrow bandpass filter with a wavelength range of 1000nm-1200nm. The filter 1615 allows light with a wavelength in the range of 1000nm-1200nm to pass through, which helps to remove the interference of excitation light and background fluorescence, thereby improving the measurement accuracy and stability of the system. The near-infrared photoelectric converter 1616 is one of an indium gallium arsenide photodiode, a photodiode or a photomultiplier tube; the detection wavelength range of the near-infrared photoelectric converter 1616 is 800nm-1700nm, covering the wavelength range allowed by the filter 1615, ensuring the effective conversion of the signal.

[0030] The fluorescent immunoassay quantitative analyzer uses a near-infrared photoelectric converter 1616 to replace the visible photoelectric sensor, which can efficiently capture the near-infrared fluorescence emitted by the fluorescent substance on the reagent card 17; the strong penetration and resistance to background fluorescence interference of near-infrared fluorescence can effectively improve the signal-to-noise ratio of the device detection, thereby greatly improving the detection sensitivity of the device.

[0031] In this embodiment, the information display system includes a printer 12 and a touch screen display 11 for displaying operation data information. The printer 12 is used to print out important operation data, test results or other related information so that users or operators can obtain paper documents for recording or archiving; the interactivity of the touch screen display 11 makes the operation more convenient and efficient.

[0032] The control circuit system includes a control circuit board 13, and the near-infrared fluorescence detection system, material conveying and fixing system, printer 12 and touch screen 11 are electrically connected to the control circuit board 13. The control circuit system is centered on the control circuit board 13 and is responsible for controlling and adjusting the work of each system.

[0033] In this embodiment, the material conveying and fixing system includes a reagent card conveying track 15, a card slot 14 located on the reagent card conveying track 15, and a stepping drive motor for driving the card slot 14 to move parallel to the reagent card conveying track 15, wherein the card slot 14 is used to carry and place the reagent card 17. The reagent card conveying track 15 serves as a channel for conveying the reagent card 17, and is used to guide the reagent card 17 from the placement port of the system to the detection position; the design of the card slot 14 takes into account the size and shape of the reagent card 17, and the size and shape of the card slot 14 are adapted to the size and shape of the reagent card 17, ensuring that the reagent card 17 can be firmly fixed in the card slot 14 to avoid shaking or tilting during movement.

[0034] The front end of the box body 10 is provided with a reagent card placement port that is connected to the reagent card conveying track 15 of the material conveying and fixing system. The card slot 14 slides in and out through the reagent card placement port, which is convenient for users or operators to place and remove reagent cards 17.

[0035] During the actual detection process, the reagent card 17 is inserted into the card slot 14. Driven by the stepper drive motor, the reagent card 17 moves along the reagent card conveying track 15 and is fixed in the photosensitive area of the near-infrared photoelectric converter 1616; the infrared light source 1611 emits 808nm excitation light in the horizontal direction through the first lens 1612 to reach the spectroscope 1613; the spectroscope 1613 irradiates the 808nm excitation light to the window area of the reagent card 17 through the second lens 1614, thereby stimulating the fluorescent substances specifically aggregated in the T band and C band areas on the nitrocellulose membrane of the reagent card 17, and then generates vertical near-infrared fluorescence respectively. The near-infrared fluorescence passes through the second lens 1614 and then passes through the filter 1615 to remove the interference of the excitation light and background fluorescence, and is finally captured by the near-infrared photoelectric converter 1616 and converted into an electrical signal. After being processed and calculated by the software algorithm, the sample detection result is finally presented on the touch display screen 11, and the printer 12 can print out the sample detection result in real time.

[0036] The above description is only a preferred embodiment of the utility model and is not intended to limit the utility model. Although the utility model is described in detail with reference to the above embodiments, those skilled in the art can still modify the technical solutions recorded in the above embodiments or replace some of the technical features therein by equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the utility model shall be included in the protection scope of the utility model.

Claims

1. A near-infrared fluorescent immunoassay quantitative analyzer, characterized in that: It comprises a box (10), an information display system fixed on the surface of the box (10), and a control circuit system, a material conveying and fixing system, and a near-infrared fluorescence detection system fixed inside; the information display system, the material conveying and fixing system, and the near-infrared fluorescence detection system are all electrically connected to the control circuit system; The near-infrared fluorescence detection system is used to excite and collect near-infrared fluorescence emission signals, and the near-infrared fluorescence detection system is located on the upper part of the material conveying and fixing system; the near-infrared fluorescence detection system comprises an infrared light source (1611), a first lens (1612), a spectroscope (1613), a second lens (1614), a filter (1615) and a near-infrared photoelectric converter (1616); the infrared light source (1611) is located on the same horizontal line as the first lens (1612) and the spectroscope (1613); the second lens (1614), the spectroscope (1613), the filter (1615) and the near-infrared photoelectric converter (1616) are arranged in sequence from bottom to top; the light emission direction of the infrared light source (1611) and the light sensing direction of the near-infrared photoelectric converter (1616) are perpendicular to each other and are located in the same optical path plane; the spectroscope (1613) is arranged in the optical path plane and is located at the intersection of the light emission direction and the light sensing direction.

2. A near-infrared fluorescent immunoassay quantitative analyzer according to claim 1, characterized in that: The centers of the second lens (1614), the beam splitter (1613), the filter (1615) and the near-infrared photoelectric converter (1616) are located on the same vertical line.

3. A near-infrared fluorescent immunoassay quantitative analyzer according to claim 1, characterized in that: The beam splitter (1613) is arranged at an inclined angle of 45 degrees.

4. A near-infrared fluorescent immunoassay quantitative analyzer according to claim 1, characterized in that: The infrared light source (1611) is one of a halogen tungsten lamp, a light emitting diode or a laser diode; the excitation wavelength of the infrared light source is 600nm-900nm.

5. A near-infrared fluorescent immunoassay quantitative analyzer according to claim 1, characterized in that: The filter (1615) is a narrow-band pass filter with a wavelength range of 1000nm-1200nm.

6. A near-infrared fluorescent immunoassay quantitative analyzer according to claim 1, characterized in that: The near-infrared photoelectric converter (1616) is one of an indium gallium arsenide photodiode, a photodiode or a photomultiplier tube; the detection wavelength range of the near-infrared photoelectric converter (1616) is 800nm-1700nm.

7. A near-infrared fluorescent immunoassay quantitative analyzer according to claim 1, characterized in that: The information display system comprises a printer (12) and a touch display screen (11) for displaying operating data information.

8. A near-infrared fluorescent immunoquantitative analyzer according to claim 7, characterized in that: The control circuit system comprises a control circuit board (13), and the near-infrared fluorescence detection system, the material conveying and fixing system, the printer (12) and the touch display screen (11) are electrically connected to the control circuit board (13).

9. A near-infrared fluorescent immunoquantitative analyzer according to claim 8, characterized in that: The material conveying and fixing system comprises a reagent card conveying track (15), a card slot (14) located on the reagent card conveying track (15), and a stepping drive motor for driving the card slot (14) to move parallel to the reagent card conveying track (15).

10. A near-infrared fluorescent immunoquantitative analyzer according to claim 9, characterized in that: The front end of the box (10) is provided with a reagent card placement opening that is connected to the reagent card conveying track (15), and the card slot (14) slides in and out through the reagent card placement opening.