Device for monitoring growth curve of microbial thalli on line in real time

Abstract

The utility model discloses an on-line real-time monitoring microbial thallus growth curve device, which comprises a device bottom plate, a support frame, a culture fermentation tank and a spectrophotometer, the support frame is fixed on one side of the top end of the device bottom plate, the culture fermentation tank is arranged inside the support frame, a sealing cover plate is arranged above the support frame, and the spectrophotometer is arranged on the sealing cover plate. A rotary driving part is installed at the center position of the top end of the sealing cover plate, the output end of the rotary driving part extends to the position below the sealing cover plate and is provided with a stirring paddle, a telescopic driving part is installed at the position, on one side of the rotary driving part, of the top end of the sampling barrel, and the output end of the telescopic driving part is fixedly connected with a piston in the sampling barrel; and the spectrophotometer is arranged at the top end of the device bottom plate on one side of the support frame. According to the utility model, the monitoring time interval can be set, stirring can be carried out before sampling, the concentration and optical density (OD) value of bacterial suspension can be measured through automatic timing sampling, the operation is simple and convenient, and the problem of complexity in measurement of a thallus growth curve is solved.

Description

Technical Field

[0001] The utility model relates to the technical field of microbial monitoring, in particular to an online real-time monitoring device for microbial cell growth curves. Background Art

[0002] Microbial growth usually refers to the increase in the number of cells. The microbial growth curve is a curve with the growth amount of microorganisms as the ordinate and the culture time as the abscissa. It represents the dynamics of the entire process of bacterial growth, reproduction, aging and death. Microbial growth has certain regularity. The typical microbial growth curve includes four periods: lag phase, logarithmic phase, stable phase and decay phase. The growth curve can measure important indicators such as the metabolism of microorganisms under different growth conditions and the growth trend of microorganisms.

[0003] The Chinese patent with the publication number CN218811756U discloses a multifunctional microbial growth curve measuring instrument, including a measuring instrument and a constant temperature box, wherein the interior of the measuring instrument is fixedly connected to the outer wall of the constant temperature box, the measuring instrument includes a body, the interior of the body is fixedly connected to the outer wall of the constant temperature box, a connecting shaft is fixedly connected to the surface of the body, one side of the connecting shaft is fixedly connected to a dust cover, a square groove is provided on the bottom surface of the dust cover, and the constant temperature box includes a box body and a box cover, and the outer wall of the box body is fixedly connected to the interior of the body.

[0004] Although the above-mentioned measuring instrument can monitor microorganisms and the monitored growth curve is clearly displayed on the display screen, it is inconvenient to set the monitoring time interval, it is inconvenient to stir before sampling, the sample is not reliable enough, and it is inconvenient to automatically take samples at regular intervals to measure the concentration and optical density OD value of the bacterial suspension. The degree of automation is not high, and the measurement of the bacterial growth curve is relatively cumbersome. Therefore, we propose an online real-time monitoring device for microbial growth curves. Utility Model Content

[0005] The purpose of the utility model is to provide an online real-time monitoring device for the growth curve of microorganisms to solve the problems raised in the above background technology.

[0006] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: an online real-time monitoring device for microbial cell growth curve, comprising a device bottom plate, a support frame, a culture fermentation tank and a spectrophotometer, wherein the support frame is fixed to one side of the top of the device bottom plate, the culture fermentation tank is arranged inside the support frame, a sealing cover plate is arranged above the support frame, and the sealing cover plate is tightly fitted with the culture fermentation tank, a rotating drive member is installed at the center position of the top of the sealing cover plate, and the output end of the rotating drive member extends to the bottom of the sealing cover plate and is installed with a stirring paddle, a telescopic drive member is installed at the top of a sampling tube on one side of the rotating drive member, and the output end of the telescopic drive member is fixedly connected to a piston inside the sampling tube, the bottom end of the sampling tube passes through the sealing cover plate and is installed with a sampling tube, the spectrophotometer is arranged at the top of the device bottom plate on one side of the support frame, and a control screen is installed at the top of the spectrophotometer, a measuring slot is arranged on one side inside the spectrophotometer, and a sample bottle is arranged inside the measuring slot.

[0007] Preferably, a liquid adding pipe is provided on an outer wall at the top of the culture fermentation tank for liquid adding.

[0008] Preferably, a gas inlet pipe is provided on an outer wall at the bottom of the culture fermentation tank, and a gas outlet pipe is provided on another outer wall at the top of the culture fermentation tank for gas supply and exhaust.

[0009] Preferably, mounting bolts are provided at the corner positions inside the sealing cover plate, and the mounting bolts are threadedly connected to the threaded holes at the top of the support frame for fixing the sealing cover plate.

[0010] Preferably, an infusion tube is installed on the outer wall of the bottom of the sampling tube, and the bottom end of the infusion tube extends to the inside of the sample bottle for liquid transportation.

[0011] Preferably, a second valve is installed at the connection between the sampling tube and the sampling cylinder to facilitate controlling the connectivity state of the sampling tube.

[0012] Preferably, a first valve is installed at the connection between the infusion tube and the sampling cylinder to facilitate controlling the connectivity state of the infusion tube.

[0013] Compared with the prior art, the beneficial effects of the utility model are:

[0014] By opening the gas inlet tube, nitrogen or carbon dioxide is filled into the culture fermentation tank, and the gas in the culture fermentation tank is discharged through the gas exhaust pipe to achieve the purpose of emptying oxygen and meet the anaerobic needs of microbial cells. Similarly, oxygen is filled through the gas inlet tube to meet the aerobic needs; liquid microorganisms are poured into the culture fermentation tank through the liquid adding tube. The external controller can set the monitoring time interval, and the rotating drive member and the telescopic drive member are controlled to work. The rotating drive member drives the stirring paddle to rotate, and the liquid can be stirred before sampling. The telescopic drive member drives the piston inside the sampling tube to move upward, and the second valve is opened, so that the sampling tube draws the liquid into the sampling tube. The second valve is closed, the first valve is opened, and the telescopic drive member drives the piston downward, so that the liquid flows into the sample bottle inside the measuring tank through the infusion tube, completing the automatic sampling work, and the concentration of the bacterial suspension and the optical density OD value are measured by the spectrophotometer and the data are summarized. The curve is drawn and displayed on the control screen. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 It is a cross-sectional structural schematic diagram of the utility model;

[0016] Figure 2 This is a schematic diagram of the enlarged structure of the culture fermentation tank of the utility model;

[0017] Figure 3 It is a schematic diagram of the enlarged structure of the spectrophotometer of the utility model;

[0018] Figure 4 It is a schematic diagram of an enlarged top view of the support frame of the utility model.

[0019] In the figure: 1. device bottom plate; 2. support frame; 201. threaded hole; 3. culture fermentation tank; 301. gas inlet pipe; 302. liquid adding pipe; 303. gas exhaust pipe; 4. sealing cover plate; 401. mounting bolt; 5. rotating drive member; 6. stirring paddle; 7. telescopic drive member; 8. sampling cylinder; 9. piston; 10. sampling tube; 11. spectrophotometer; 12. control screen; 13. first valve; 14. infusion tube; 15. second valve; 16. measuring tank; 17. sample bottle. DETAILED DESCRIPTION

[0020] The technical solutions in the embodiments of the utility model will be clearly and completely described below in conjunction with the drawings in the embodiments of the utility model. All other embodiments obtained by ordinary technicians in the field without creative work based on the embodiments of the utility model shall fall within the scope of protection of the utility model.

[0021] See also Figure 1-4, the utility model provides an embodiment: an online real-time monitoring device for the growth curve of microorganisms, comprising a device bottom plate 1, a support frame 2, a culture fermentation tank 3 and a spectrophotometer 11, the support frame 2 is fixed to one side of the top of the device bottom plate 1, the culture fermentation tank 3 is arranged inside the support frame 2, a sealing cover plate 4 is arranged above the support frame 2, the sealing cover plate 4 is tightly fitted with the culture fermentation tank 3, a rotating drive member 5 is installed at the center position of the top of the sealing cover plate 4, and the output end of the rotating drive member 5 extends to the bottom of the sealing cover plate 4 and is installed with a stirring paddle 6, a telescopic drive member 7 is installed at the top of a sampling tube 8 on one side of the rotating drive member 5, and the output end of the telescopic drive member 7 is fixedly connected to a piston 9 inside the sampling tube 8, and the bottom end of the sampling tube 8 passes through the sealing cover plate 4 and is installed with a sampling tube 10;

[0022] Specifically, the external controller can set the monitoring time interval, and by controlling the operation of the rotary drive member 5 and the telescopic drive member 7, the rotary drive member 5 drives the stirring paddle 6 to rotate, so that the liquid can be stirred before sampling, and the telescopic drive member 7 drives the piston 9 inside the sampling tube 8 to move upward, and the second valve 15 is opened, so that the sampling tube 10 draws the liquid into the sampling tube 8, the second valve 15 is closed, the first valve 13 is opened, and the telescopic drive member 7 drives the piston 9 to move downward, so that the liquid flows into the sample bottle 17 inside the measuring tank 16 through the infusion tube 14, and the automatic sampling work is completed;

[0023] The spectrophotometer 11 is arranged at the top of the device bottom plate 1 on one side of the support frame 2, and a control screen 12 is installed at the top of the spectrophotometer 11, and a measuring tank 16 is arranged at one side inside the spectrophotometer 11, and a sample bottle 17 is arranged inside the measuring tank 16;

[0024] Specifically, the light source in the measuring tank 16 passes through the liquid, and then the detector is used to measure the intensity difference between the transmitted light and the incident light, and the optical density OD value of the liquid can be calculated. The greater the content of microorganisms in the bacterial suspension, the less light is transmitted through the suspension, and the more light is scattered, that is, the concentration of the bacterial suspension is inversely proportional to the transmittance within a certain range and is proportional to the optical density. First, a concentration absorbance curve is made using several standard concentrations of bacterial liquid, and then the absorbance of the bacterial liquid in the measuring tank 16 is measured, and the concentration of the bacterial suspension can be inversely deduced, so that the bacterial suspension concentration and the optical density OD value can be automatically sampled and measured at regular intervals. The spectrophotometer 11 summarizes the data, and the curve is drawn and displayed on the control screen 12;

[0025] A liquid adding pipe 302 is provided on an outer wall of the top of the culture fermentation tank 3 for liquid adding;

[0026] A gas inlet pipe 301 is provided on one outer wall at the bottom of the culture fermentation tank 3, and a gas outlet pipe 303 is provided on another outer wall at the top of the culture fermentation tank 3 for gas transmission and exhaust;

[0027] Specifically, the gas inlet pipe 301 is opened, and nitrogen or carbon dioxide is charged into the culture fermentation tank 3. The gas in the culture fermentation tank 3 is discharged through the gas discharge pipe 303 to achieve the purpose of exhausting oxygen and meet the anaerobic needs of the microbial cells. Similarly, oxygen is charged through the gas inlet pipe 301 to meet the aerobic needs.

[0028] The corners of the sealing cover plate 4 are all penetrated with mounting bolts 401, and the mounting bolts 401 are threadedly connected with the threaded holes 201 at the top of the support frame 2, so as to fix the sealing cover plate 4;

[0029] Specifically, the culture fermentation tank 3 is placed inside the support frame 2, the sealing cover plate 4 is covered on the top of the culture fermentation tank 3, the stirring paddle 6 and the sampling tube 10 are inserted into the culture fermentation tank 3, and the mounting bolts 401 are screwed into the corresponding threaded holes 201, so that the sealing cover plate 4 is fixed on the top of the support frame 2;

[0030] An infusion tube 14 is installed on the outer wall of the bottom of the sampling tube 8, and the bottom end of the infusion tube 14 extends to the inside of the sample bottle 17 for liquid transportation;

[0031] A second valve 15 is installed at the connection between the sampling tube 10 and the sampling cylinder 8 to facilitate controlling the connection state of the sampling tube 10;

[0032] A first valve 13 is installed at the connection between the infusion tube 14 and the sampling tube 8 to facilitate controlling the connection state of the infusion tube 14 .

[0033] When the embodiment of the present application is used: first, the device is mainly composed of a culture fermentation tank 3 and a spectrophotometer 11, the culture fermentation tank 3 is placed on the inner side of the support frame 2, the sealing cover plate 4 is covered on the top of the culture fermentation tank 3, the stirring paddle 6 and the sampling tube 10 are extended into the culture fermentation tank 3, the mounting bolt 401 is screwed into the corresponding threaded hole 201, so that the sealing cover plate 4 is fixed to the top of the support frame 2, and then, the gas inlet pipe 301 is opened, nitrogen or carbon dioxide is filled into the culture fermentation tank 3, and the gas in the culture fermentation tank 3 is discharged through the gas discharge pipe 303. The purpose of exhausting oxygen is achieved to meet the anaerobic needs of microorganisms. Similarly, oxygen is charged through the gas inlet pipe 301 to meet the aerobic needs. Then, the liquid adding pipe 302 is opened, and the liquid microorganisms are poured into the culture fermentation tank 3 through the liquid adding pipe 302. The external controller can set the monitoring time interval, and the rotating driving member 5 and the telescopic driving member 7 are controlled to work. The rotating driving member 5 drives the stirring paddle 6 to rotate, and the liquid can be stirred before sampling. The telescopic driving member 7 drives the piston 9 inside the sampling tube 8 to move upward, and the second valve 15 is opened, so that the sampling tube 1 0 The liquid is drawn into the sampling tube 8, the second valve 15 is closed, the first valve 13 is opened, and the telescopic driving member 7 drives the piston 9 to move downward, so that the liquid flows into the sample bottle 17 inside the measuring tank 16 through the infusion tube 14, and the automatic sampling work is completed. The light source in the measuring tank 16 passes through the liquid, and then the detector is used to measure the intensity difference between the transmitted light and the incident light, and the optical density OD value of the liquid can be calculated. The greater the content of microorganisms in the bacterial suspension, the less light is transmitted through the suspension, and the more light is scattered, that is, the concentration of the bacterial suspension is within a certain range and the intensity difference between the transmitted light and the incident light is within a certain range. The light intensity is inversely proportional to the optical density and is directly proportional to the optical density. A concentration absorbance curve is first made with several standard concentrations of bacterial solution, and then the absorbance of the bacterial solution in the measuring tank 16 is measured to infer the concentration of the bacterial suspension. Thus, the bacterial suspension concentration and the optical density OD value can be automatically and regularly sampled and measured. The spectrophotometer 11 summarizes the data, and the curve is drawn and displayed on the control screen 12. The device can set the monitoring time interval, stir before sampling, and automatically and regularly sample and measure the bacterial suspension concentration and the optical density OD value. The operation is simple and convenient, thereby solving the cumbersome problem of measuring the bacterial growth curve.

[0034] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. For those skilled in the art, the present invention may be subject to various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. An online real-time monitoring device for microbial cell growth curve, characterized in that: The device comprises a device bottom plate (1), a support frame (2), a culture fermentation tank (3) and a spectrophotometer (11), wherein the support frame (2) is fixed to one side of the top of the device bottom plate (1), the culture fermentation tank (3) is arranged inside the support frame (2), a sealing cover plate (4) is arranged above the support frame (2), the sealing cover plate (4) is tightly fitted with the culture fermentation tank (3), a rotating driving member (5) is installed at the center position of the top of the sealing cover plate (4), and the output end of the rotating driving member (5) extends to the bottom of the sealing cover plate (4) and is installed with a stirring paddle (6), and the rotating driving member ( 5) is provided with a telescopic driving member (7) at the top end of the sampling tube (8) on one side, and the output end of the telescopic driving member (7) is fixedly connected to the piston (9) inside the sampling tube (8), the bottom end of the sampling tube (8) passes through the sealing cover plate (4) and is provided with a sampling tube (10), the spectrophotometer (11) is arranged at the top end of the device bottom plate (1) on one side of the support frame (2), and the top end of the spectrophotometer (11) is provided with a control screen (12), a measuring tank (16) is provided on one side inside the spectrophotometer (11), and a sample bottle (17) is provided inside the measuring tank (16).

2. The device for online real-time monitoring of microbial growth curve according to claim 1, characterized in that: A liquid adding pipe (302) is arranged on an outer wall at the top of the culture fermentation tank (3).

3. The device for online real-time monitoring of microbial cell growth curve according to claim 1, characterized in that: A gas inlet pipe (301) is arranged on an outer wall at the bottom of the culture fermentation tank (3), and a gas outlet pipe (303) is arranged on another outer wall at the top of the culture fermentation tank (3).

4. The device for online real-time monitoring of microbial cell growth curve according to claim 1, characterized in that: The corner positions inside the sealing cover plate (4) are all penetrated with mounting bolts (401), and the mounting bolts (401) are threadedly connected to the threaded holes (201) at the top of the support frame (2).

5. The device for online real-time monitoring of microbial growth curve according to claim 1, characterized in that: A liquid infusion tube (14) is installed on the outer wall of the bottom of the sampling tube (8), and the bottom end of the liquid infusion tube (14) extends to the inside of the sample bottle (17).

6. The device for online real-time monitoring of microbial growth curve according to claim 1, characterized in that: A second valve (15) is installed at the connection between the sampling tube (10) and the sampling cylinder (8).

7. The device for online real-time monitoring of microbial cell growth curve according to claim 5, characterized in that: A first valve (13) is installed at the connection between the liquid infusion tube (14) and the sampling cylinder (8).

Images