Sperm counting plate

Abstract

A sperm counting plate comprises a bottom plate and a top plate, a coating and a connecting layer are arranged between the bottom plate and the top plate, the coating is fixed to the bottom plate, a plurality of hollow holes are formed in the coating, and a plurality of counting cavities used for counting sperms are formed among the bottom plate, the holes in the coating and the top plate. Each counting cavity is provided with a sample inlet and a gas outlet, and the connecting layer is used for fixing the top plate on the coating; a plurality of particles with the same height are uniformly distributed on the connecting layer, the two ends of each particle in the height direction are in contact with the top plate and the coating respectively, and the particles are connected with one another through viscous substances; the connecting layer is composed of a plurality of connecting parts, and the shape of each connecting part corresponds to the shape of the cavity wall of the corresponding counting cavity.

Description

Technical Field

[0001] The utility model relates to the field of cytological counting, in particular to a sperm counting plate. Background Art

[0002] Sperm concentration and motility are basic parameters for routine semen analysis, and the sperm counting plate is one of the essential tools for analyzing sperm concentration and motility. At present, in addition to the bovine blood cell counting plate recommended by the WHO Laboratory Manual for the Examination and Processing of Human Semen, there are mainly two types of sperm counting plates on the market. One is a reusable sperm counting plate made of a metal substrate and a glass cover, and the other is a disposable sperm counting plate. The reusable sperm counting plate can usually only process one specimen at a time and needs to be thoroughly cleaned after use, which is troublesome to use and inefficient.

[0003] The existing disposable sperm counting plate, such as a sperm cell counting slide disclosed in Chinese patent publication number CN213041748U, comprises, from bottom to top, a slide, a support layer and a cover glass, wherein the support layer is provided with at least one recessed portion containing an injection port, the cover glass is fixed on the support layer so that the recessed portion forms a counting cavity, and the injection port at least partially exposes the edge of the cover glass; the support layer is made of a super-hydrophobic resin material, and a microporous structure is formed on the surface; the support layer also has a glue layer coated on the upper surface of the support layer and located outside the counting cavity, and the cover glass is bonded to the support layer through the glue layer; the support layer is provided with a glue coating cavity, and the glue coating cavity is coated with a glue layer, and the slide glass is fixed to the slide glass through the glue layer.

[0004] The sperm cell counting slide disclosed in the above patent has its layers connected by glue and has no ventilation holes. The glue connection is prone to the defect that glue penetrates into the counting cavity, and the structure without ventilation holes will lead to slow liquid inflow speed, which will eventually lead to problems such as poor liquid inflow, blockage, and inability of the counting cavity to be filled with liquid. Utility Model Content

[0005] In order to solve the problems existing in the prior art, the utility model discloses a sperm counting plate.

[0006] A sperm counting plate, comprising a bottom plate and a top plate, a coating and a connecting layer are arranged between the bottom plate and the top plate, the coating is fixed on the bottom plate, a plurality of hollow holes are arranged on the coating, a plurality of counting cavities for counting semen are formed between the bottom plate, the holes on the coating and the top plate, each of the counting cavities is provided with a sample inlet and an air outlet, and the connecting layer is used to fix the top plate on the coating;

[0007] The connecting layer is evenly distributed with a number of particles of equal height, and the two ends of each particle in the height direction are respectively in contact with the top plate and the coating, and the particles are connected to each other through a sticky substance; the connecting layer is composed of a number of connecting parts, and the shape of each connecting part corresponds to the shape of the cavity wall of the corresponding counting cavity.

[0008] Specifically, each counting chamber is independent of each other, and each counting chamber is provided with a sample inlet and an air outlet, so the sperm counting plate provided by the utility model can be used to count multiple samples at one time;

[0009] The depth of each counting cavity is the sum of the coating thickness and the connecting layer thickness. Since the connecting layer is evenly distributed with a number of particles of equal height, and both ends of each particle of equal height are in contact with the top plate and the coating, the uniformity of the connecting layer thickness is achieved. Therefore, after ensuring the uniformity of the coating thickness, the depth of each counting cavity can achieve uniformity. For a single counting cavity, since the depth is basically equal at each location, the probability of counting errors is greatly reduced.

[0010] In addition, in actual use, since the sizes of different sperm are different (for example, there is a significant difference in the sperm sizes of humans and other animals, and there are also differences in the sperm sizes of other animals), the depth of the counting chamber must be high enough to ensure that the movement of the sperm is not compressed, thereby affecting the judgment of motility and vitality, and low enough to ensure that the sperm is evenly distributed in a single layer, does not overlap with each other, and is in the same focal plane. Therefore, counting chambers of different depths are required for sperm of different sizes (that is, sperm counting plates need to have multiple models), and counting chambers of different depths can be obtained by selecting particles of different heights.

[0011] Preferably, each of the counting chambers is arranged in sequence along the length direction of the bottom plate, and the inlet and the air outlet of each counting chamber are arranged at opposite ends of the counting chamber, and the inlet and the air outlet are arranged close to the edge of the bottom plate, and the width of the top plate is smaller than the distance between the inlet and the air outlet.

[0012] Specifically, the sample inlet and the gas outlet are arranged near the edge of the bottom plate and at both ends of the counting chamber, so that the semen can fill the entire counting chamber more smoothly; the width of the top plate is smaller than the distance between the sample inlet and the gas outlet, so that the sample inlet and the gas outlet are at least partially exposed. This structure facilitates the addition of semen into the counting chamber and also allows the gas in the counting chamber to be discharged more smoothly from the gas outlet.

[0013] Preferably, the injection port is provided with a stop block to prevent semen from flowing in a direction away from the counting chamber; the stop block is arc-shaped and protrudes in a direction away from the counting chamber, and both ends of the stop block are integrally connected to the cavity wall of the injection port.

[0014] Specifically, during use, the sperm counting plate is placed horizontally on the workbench. When semen is added from the injection port, the semen in a fluid state will flow in all directions. In order to ensure that the semen can flow into the counting chamber, it is appropriate to set a stop block structure.

[0015] Preferably, the distances between the center points of adjacent injection ports are equal to each other, and the distances between the center points of adjacent gas outlets are equal to each other; the distance between the center points of adjacent injection ports is 9-18 mm; the distance between the center points of adjacent gas outlets is 9-18 mm.

[0016] Specifically, at this distance, it can be ensured that semen will not be connected in series to adjacent injection ports when adding samples, and the speed of liquid capillary action can be increased, thereby accelerating the speed of semen filling the counting chamber.

[0017] Preferably, the cavity wall of each counting cavity is streamlined.

[0018] Specifically, if the connection between the walls of the counting chamber is not smooth enough, it is very likely that the semen will not be able to completely fill the counting chamber, and the failure of the semen to completely fill the counting chamber will lead to counting errors. Therefore, it is appropriate to design the walls of the counting chamber to be streamlined.

[0019] Preferably, the adhesive substance of the connecting layer becomes a non-adhesive substance after coagulation.

[0020] Specifically, semen is added to the counting chamber after the connecting layer is solidified. The solidified connecting layer ensures that the depth of the counting chamber will not change. At the same time, the solidified connecting layer has biocompatibility and can directly contact with semen. However, the contents of the solidified connecting layer will not dissolve into the semen to form impurities, so there will be no problems of unclear imaging field of view or impact on the motility and vitality of sperm. At the same time, since the viscous substance is located in the gap between the particles, and the size of the particles is small enough, the amount of viscous substance used is small enough, and there are molecular forces between the particles, it can effectively prevent the viscous substance from penetrating into the counting chamber, and then there will be no problem of poor liquid inflow and blockage caused by the viscous substance penetrating into the counting chamber. The viscous substance can be silicone, methacrylate resin or other synthetic resins.

[0021] Preferably, visual windows are provided at positions of the top plate and the bottom plate corresponding to the counting chamber; and a counting grid is etched on the visual window of the top plate.

[0022] Specifically, under this structure, the operator can conveniently observe the flow of semen, and can also directly observe through the visual window whether the semen has completely filled the counting chamber, which is also convenient for counting; and the structure of the counting grid is more convenient for counting;

[0023] Typically, the top plate and the bottom plate are both made of light-transmitting materials, preferably high-definition and high-transmittance optical glass.

[0024] Preferably, the coating is also provided with an information recording area, and the information recording area records information by writing or coding.

[0025] Specifically, setting up an information recording area for recording information can make it easier to manage samples in an information-based manner, and facilitate verification and traceability.

[0026] Compared with the prior art, the utility model is beneficial in that:

[0027] (1) The height of each particle evenly distributed in the connecting layer is uniform. When the coating thickness is uniform, the depth of each technical cavity can be guaranteed to be uniform. For a single counting cavity, the structure with the same depth everywhere can effectively reduce the counting error. At the same time, after the viscous material is solidified, the connecting layer will not dissolve into the sample, which solves the problem that the glue easily penetrates into the counting cavity and causes poor liquid inflow and blockage.

[0028] (2) The structure of the sample inlet and the gas outlet is set, and the distance between the center points of the adjacent sample inlets and the gas outlet is kept at an appropriate distance, which ensures that the semen will not flow into the adjacent sample inlet when the semen is added. At the same time, it can also increase the speed of the liquid capillary action, thereby increasing the speed of the semen filling the technical cavity.

[0029] (3) Setting up the structure of the information recording area The structure used to record information can make it easier to manage samples in an information-based manner and facilitate verification and traceability. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Figure 1 This is a front view of the sperm counting board disclosed in the utility model;

[0031] Figure 2 It is a top view of the sperm counting plate disclosed in the utility model;

[0032] Figure 3 It is an exploded view of the sperm counting plate disclosed in the utility model;

[0033] Figure 4 It is a full cross-sectional view of the sperm counting plate disclosed in the utility model in the main viewing direction. DETAILED DESCRIPTION

[0034] The utility model is further described below in conjunction with the accompanying drawings and specific embodiments.

[0035] like Figure 1-4As shown, a sperm counting plate comprises a bottom plate 10 and a top plate 20, a coating 30 and a connecting layer 40 are arranged between the bottom plate 10 and the top plate 20, the coating 30 is fixed on the bottom plate 10, a plurality of hollow holes are arranged on the coating 30, a plurality of counting cavities 50 for counting semen are formed between the bottom plate 10, the holes on the coating 30 and the top plate 20, each counting cavity 50 is provided with a sample inlet 51 and an air outlet 52, and the connecting layer 40 is used to fix the top plate 20 on the coating 30;

[0036] The connecting layer 40 is evenly distributed with a number of particles 42 of equal height, and both ends of each particle 42 in the height direction are in contact with the top plate 20 and the coating 30 respectively, and each particle 42 is connected to each other through a sticky substance 43; the connecting layer 40 is composed of a number of connecting parts 41, and the shape of each connecting part 41 corresponds to the shape of the cavity wall of the corresponding counting cavity 50.

[0037] Each counting chamber 50 is independent of each other, and each counting chamber 50 is provided with a sample inlet 51 and an air outlet 52, so the sperm counting plate provided in this embodiment can be used to count multiple samples at one time;

[0038] The depth of each counting cavity 50 is the sum of the thickness of the coating 30 and the thickness of the connecting layer 40. Since the connecting layer 40 is evenly distributed with a number of particles 42 of equal height, and both ends of each particle 42 of equal height are in contact with the top plate 20 and the coating 30, the uniformity of the thickness of the connecting layer 40 is achieved. Therefore, after ensuring the uniformity of the thickness of the coating 30, the depth of each counting cavity 50 can achieve uniformity. For a single counting cavity 50, since the depths are basically equal at each location, the probability of counting errors is greatly reduced.

[0039] In addition, in actual use, since the sizes of different sperms are not the same (for example, there is a significant difference in the sperm sizes of humans and other animals, and there are also differences in the sperm sizes of other animals), the depth of the counting chamber 50 must be high enough to ensure that the movement of the sperm is not compressed, thereby affecting the judgment of motility and vitality, and low enough to ensure that the sperm is evenly distributed in a single layer, does not overlap with each other, and is in the same focal plane. Therefore, counting chambers 50 of different depths are required for sperms of different sizes (that is, multiple models of sperm counting plates are required), and counting chambers 50 of different depths can be obtained by selecting particles 42 of different heights.

[0040] Each counting chamber 50 is arranged in sequence along the length direction of the bottom plate 10, and the sample inlet 51 and the gas outlet 52 of each counting chamber 50 are arranged at opposite ends of the counting chamber 50, and the sample inlet 51 and the gas outlet 52 are arranged close to the edge of the bottom plate 10, and the width of the top plate 20 is smaller than the distance between the sample inlet 51 and the gas outlet 52.

[0041] The structure in which the sample inlet 51 and the gas outlet 52 are arranged near the edge of the bottom plate 10 and at both ends of the counting chamber 50 allows the semen to fill the entire counting chamber 50 more smoothly; the width of the top plate 20 is smaller than the distance between the sample inlet 51 and the gas outlet 52 so that the sample inlet 51 and the gas outlet 52 are at least partially exposed. This structure makes it convenient to add semen to the counting chamber 50, and also allows the gas in the counting chamber 50 to be discharged more smoothly from the gas outlet 52.

[0042] The injection port 51 is provided with a stopper 53 to prevent semen from flowing away from the counting chamber 50 ; the stopper 53 is arc-shaped and protrudes away from the counting chamber 50 , and both ends of the stopper 53 are integrally connected to the cavity wall of the injection port 51 .

[0043] During use, the sperm counting plate is placed horizontally on the workbench. When semen is added from the injection port 51, the semen in a fluid state will flow in all directions. In order to ensure that the semen can flow into the counting chamber 50, it is appropriate to set a stop block 53.

[0044] The distances between the center points of adjacent injection ports 51 are equal to each other, and the distances between the center points of adjacent gas outlets 52 are equal to each other; the distance between the center points of adjacent injection ports 51 is 9-18 mm; the distance between the center points of adjacent gas outlets 52 is 9-18 mm.

[0045] At this distance, it can be ensured that semen will not be connected in series to the adjacent injection port 51 when adding samples, and the speed of liquid capillary action can be increased, thereby accelerating the speed of semen filling the counting chamber 50.

[0046] The cavity wall of each counting cavity 50 is streamlined.

[0047] If the connection between the walls of the counting chamber 50 is not smooth enough, it is very likely that the semen cannot completely fill the counting chamber 50, which will lead to counting errors. Therefore, it is appropriate to design the walls of the counting chamber 50 to be streamlined.

[0048] The adhesive substance 43 of the connecting layer 40 becomes a non-adhesive substance after solidification.

[0049] That is, after the connecting layer 40 is solidified, semen is added into the counting chamber 50. The solidified connecting layer 40 ensures that the depth of the counting chamber 50 will not change. At the same time, the solidified connecting layer 40 has biocompatibility and can directly contact with semen. However, the content of the solidified connecting layer 40 will not dissolve into the semen to form impurities, so there will be no problems of unclear imaging field of view or impact on the motility and vitality of sperm. At the same time, since the viscous substance 43 is located in the gap between the particles 42 and the particles 42 are small enough, the amount of the viscous substance 43 is small enough, and there are molecular forces between the particles 42 and the particles 42, it can effectively prevent the viscous substance 43 from penetrating into the counting chamber 50, and then there will be no problem of poor liquid inflow and blockage caused by the viscous substance 43 penetrating into the counting chamber 50. The viscous substance 43 can be silicone, methacrylate resin or other synthetic resins.

[0050] Visual windows are provided at positions of the top plate 20 and the bottom plate 10 corresponding to the counting chamber 50 ; a counting grid is etched on the visual window of the top plate 20 .

[0051] Under this structure, the operator can conveniently observe the flow of semen, and can also directly observe through the visual window whether the semen has completely filled the counting chamber 50, which is also convenient for counting; and the counting grid structure is more convenient for counting;

[0052] Typically, the top plate 20 and the bottom plate 10 are both made of light-transmitting materials, preferably high-definition and high-transmittance optical glass.

[0053] The coating 30 is also provided with an information recording area 31, and the information recording area 31 records information by writing or coding.

[0054] The information recording area 31 is provided to record information, which can make it easier for information management of samples, and facilitate verification and traceability.

[0055] When in use, the sperm counting plate is placed horizontally on a workbench, and a specified volume of liquefied semen sample is added to the sample inlet 51. The semen sample flows and fills the entire counting chamber 50. After the bubbles in the counting chamber 50 and the semen sample are discharged through the air outlet 52 and the liquid is distributed more evenly, the sperm counting plate is placed under a microscope for observation and counting.

Claims

1. A sperm counting plate, comprising a bottom plate and a top plate, characterized in that: A coating and a connecting layer are provided between the bottom plate and the top plate, the coating is fixed on the bottom plate, a plurality of hollow holes are provided on the coating, a plurality of counting cavities for counting semen are formed between the bottom plate, the holes on the coating and the top plate, each of the counting cavities is provided with a sample inlet and an air outlet, and the connecting layer is used to fix the top plate on the coating; The connecting layer is evenly distributed with a number of particles of equal height, and the two ends of each particle in the height direction are respectively in contact with the top plate and the coating, and the particles are connected to each other through a sticky substance; the connecting layer is composed of a number of connecting parts, and the shape of each connecting part corresponds to the shape of the cavity wall of the corresponding counting cavity.

2. The sperm counting plate according to claim 1, characterized in that: The counting chambers are arranged in sequence along the length direction of the bottom plate, and the inlet and outlet of each counting chamber are arranged at opposite ends of the counting chamber, and the inlet and outlet are arranged close to the edge of the bottom plate, and the width of the top plate is smaller than the distance between the inlet and outlet.

3. The sperm counting plate according to claim 2, characterized in that: The sample inlet is provided with a stopper to prevent the semen from flowing in a direction away from the counting chamber.

4. The sperm counting plate according to claim 3, characterized in that: The stop block is arc-shaped and protrudes in a direction away from the counting cavity. Both ends of the stop block are integrally connected with the cavity wall of the injection port.

5. The sperm counting plate according to claim 2, characterized in that: The distances between the center points of adjacent injection ports are equal to each other, and the distances between the center points of adjacent gas outlets are equal to each other.

6. The sperm counting chamber according to claim 5, characterized in that: The distance between the center points of adjacent injection ports is 9-18 mm; the distance between the center points of adjacent gas outlets is 9-18 mm.

7. The sperm counting chamber according to claim 1, characterized in that: The cavity wall of each counting cavity is streamlined.

8. The sperm counting chamber according to claim 1, characterized in that: The adhesive substance of the connection layer becomes a non-adhesive substance after coagulation.

9. The sperm counting chamber according to claim 1, characterized in that: The top plate and the bottom plate are both provided with visual windows at positions corresponding to the counting chamber; and a counting grid is etched on the visual window of the top plate.

10. The sperm counting chamber according to claim 1, characterized in that: The coating is also provided with an information recording area, and the information recording area records information by writing or spraying codes.

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