Microfluidic experimental device

Abstract

The utility model relates to the technical field of microfluidics, and discloses a microfluidics experimental device, which solves the problems in the background technology, and comprises a substrate, the substrate is made of a transparent material, the substrate is provided with a channel, the channel is provided with two inlets and an outlet, the two inlets are communicated with each other, and the two outlets are communicated with each other. The top of the channel is also provided with a culture unit, and the culture unit is communicated with the channel, so that in the long-term imaging process, the experiment conditions can be accurately controlled, and the dynamic behaviors of fungi on the root surface and around the root surface can be captured.

Description

Technical Field

[0001] The utility model belongs to the technical field of microfluidics, in particular to a microfluidics experimental device. Background Art

[0002] Microfluidics is a field of technology and science that studies and manipulates the flow of tiny amounts of liquid (usually in the nanoliter or picoliter range) at the micrometer scale. Microfluidics combines the principles of physics, chemistry, engineering, and biology to perform complex liquid operations on an extremely small scale.

[0003] Existing experimental devices for root-fungus interactions cannot accurately control the experimental environment, such as temperature, humidity, nutrients and other parameters, making it difficult to simulate the root environment under natural conditions, which is not conducive to subsequent experimental research or analysis. Therefore, we proposed a microfluidic experimental device. Utility Model Content

[0004] In order to solve the problem that the existing experimental devices for root-fungus interaction cannot accurately control the experimental environment, such as temperature, humidity, nutrients and other parameters, and it is difficult to simulate the root environment under natural conditions, which is not conducive to subsequent experimental research or analysis, the utility model provides the following technical solutions: a microfluidic experimental device, including a substrate, the substrate is made of a transparent material, a channel is arranged on the substrate, the channel has an inlet and an outlet, the inlet has two, and a culture unit is also arranged on the top of the channel, and the culture unit is interconnected with the channel.

[0005] Preferably, one of the inlets is connected to a syringe via a polyethylene tube, and the syringe is used to inject root fungus fermentation liquid into the channel.

[0006] Preferably, the outlet is connected to a material receiving bottle via a polyethylene tube, and the material receiving bottle is provided to receive waste liquid generated during the experiment.

[0007] Preferably, the inner diameter of the channel is 160 μm.

[0008] Preferably, there are multiple channels, the structures of the multiple channels are exactly the same, the multiple channels are arranged on the substrate in parallel and evenly spaced, and the multiple channels are not interconnected, which can prevent cross contamination between channels during fungal inoculation.

[0009] Preferably, the number of the channels is 6-9.

[0010] Compared with the prior art, the beneficial effects of the utility model are:

[0011] First, the uninfected seedlings were moved into the culture unit, and nutrient solution was continuously injected through one of the inlets. Then, the cells were incubated vertically for 10-15 days. After the roots of the seedlings extended along the channel, the substrate was placed on a microscope stage as a whole. 1 mL of fluorescently labeled ectomycorrhizal fermentation liquid was pre-loaded with a syringe and injected into the channel through another inlet. A fluorescence microscope and dark-field illumination were then used to observe the behavior of the fungi in the next 30-60 minutes. This enabled the researchers to precisely control the experimental conditions during long-term imaging and capture the dynamic behavior of fungi on the root surface and its surroundings. BRIEF DESCRIPTION OF THE DRAWINGS

[0012] The accompanying drawings are used to provide a further understanding of the present invention and constitute a part of the specification. Together with the embodiments of the present invention, they are used to explain the present invention and do not constitute a limitation of the present invention. In the accompanying drawings:

[0013] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0014] Figure 2 It is a structural schematic diagram of the channel part of the utility model;

[0015] In the figure: 1. substrate; 2. channel; 3. inlet; 4. outlet; 5. culture unit; 6. syringe; 7. collecting bottle. DETAILED DESCRIPTION

[0016] The technical solutions in the embodiments of the utility model will be clearly and completely described below in conjunction with the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all of the embodiments; based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the utility model.

[0017] Depend on Figure 1-2 It is given that the utility model includes a substrate 1, which is made of a transparent material. A channel 2 is arranged on the substrate 1, and the channel 2 has an inlet 3 and an outlet 4, and there are two inlets 3. A culture unit 5 is also arranged on the top of the channel 2, and the culture unit 5 is interconnected with the channel 2.

[0018] One of the inlets 3 is connected to a syringe 6 via a polyethylene tube, and the syringe 6 is used to inject root fungus fermentation liquid into the channel 2 .

[0019] The outlet 4 is connected to a material receiving bottle 7 via a polyethylene tube, and the material receiving bottle 7 is provided to receive waste liquid generated during the experiment.

[0020] The inner diameter of the channel 2 is 160 μm.

[0021] There are multiple channels 2, and the structures of the multiple channels 2 are exactly the same. The multiple channels 2 are arranged on the substrate 1 in parallel and evenly spaced. The multiple channels 2 are not connected to each other, which can prevent cross contamination between the channels 2 during fungal inoculation.

[0022] The number of the channels 2 is 6-9.

[0023] Working principle: During operation, firstly, the uninfected seedlings are moved into the culture unit 5, and the nutrient solution is continuously injected through one of the inlets 3, and then incubated vertically for 10-15 days. After the roots of the seedlings extend along the channel 2, the substrate 1 is placed on the microscope stage as a whole, and 1 mL of fluorescently labeled ectomycorrhizal fermentation liquid is pre-loaded through the syringe 6 and injected into the channel 2 through another inlet 3. Then, a fluorescent microscope and dark field illumination are used to observe the behavior of the fungi in the next 30-60 minutes, thereby achieving the ability to accurately control the experimental conditions during long-term imaging and capture the dynamic behavior of fungi on the root surface and its surroundings.

[0024] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0025] Although the embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A microfluidic experimental device, characterized in that: The invention comprises a substrate (1), wherein the substrate (1) is made of a transparent material, a channel (2) is arranged on the substrate (1), the channel (2) has an inlet (3) and an outlet (4), and the inlet (3) has two inlets; a culture unit (5) is also arranged on the top of the channel (2), and the culture unit (5) is interconnected with the channel (2).

2. A microfluidic experimental device according to claim 1, characterized in that: One of the inlets (3) is connected to a syringe (6) via a polyethylene tube, and the syringe (6) is used to inject root fungus fermentation liquid into the channel (2).

3. A microfluidic experimental device according to claim 1, characterized in that: The outlet (4) is connected to a material receiving bottle (7) via a polyethylene tube.

4. A microfluidic experimental device according to claim 1, characterized in that: The inner diameter of the channel (2) is 160 μm.

5. A microfluidic experimental device according to claim 4, characterized in that: There are a plurality of channels (2), the structures of the plurality of channels (2) are completely the same, the plurality of channels (2) are arranged on the substrate (1) in parallel and at even intervals, and the plurality of channels (2) are not interconnected.

6. A microfluidic experimental device according to claim 5, characterized in that: The number of the channels (2) is 6-9.

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