Automatic sampling device for cell culture fluid

Abstract

The utility model provides an automatic sampling device for cell culture fluid, which relates to the technical field of cell culture and comprises a bottom plate, a sampling component, a sample placing component and a moving body, the sampling component is movably connected with one side of the moving body, the sample placing component is positioned below the sampling component, and the sample placing component is movably connected with one side of the moving body. The sampling assembly, the sample placing assembly and the moving main body are all arranged above the bottom plate, for the problem of matrix arrangement type centralized culture sampling, the device can pull a plurality of first connecting rods and a plurality of second connecting rods by pulling a pull rod, and the plurality of first connecting rods and the plurality of second connecting rods are hinged end to end; the center of a first connecting rod is hinged to the center of a second connecting rod, and the first connecting rod and the second connecting rod located at the head end are hinged to a sliding block and a fixed block located at the head end respectively. And the method is not suitable for containers with large model difference.

Description

Technical Field

[0001] The utility model relates to the technical field of cell culture, in particular to an automatic sampling device for cell culture fluid. Background Art

[0002] Cell culture refers to a method of simulating the in vivo environment in vitro to enable cells to survive, grow, reproduce and maintain their main structures and functions. Cell culture is also called cell cloning technology. The formal term in biology is cell culture technology. Regardless of the entire bioengineering technology or one of its biological cloning technologies, cell culture is an indispensable process.

[0003] A Chinese patent with publication number CN219861337U discloses an automated sampling device in a cell culture fluid production process. The utility model relates to an automated sampling device in a cell culture fluid production process, comprising: a frame, a sampling mechanism is arranged inside the frame, the sampling mechanism comprises a motor, the motor is arranged at the bottom of the inner cavity of the frame, the motor is fixedly connected to a rotating rod through an output shaft, one side of the rotating rod is connected to one side of the inner cavity of the frame through a bearing, a fixed plate is fixed inside the frame, a first sleeve is arranged outside the rotating rod, the first sleeve is connected to the rotating rod through a one-way bearing, through the design of the sampling mechanism, the motor is used as a power source to drive the circular plate to rotate, and at the same time, the suction pipe and the piston can move up and down with the reciprocating screw rod to achieve the purpose of sampling, the utility model does not require manual assistance, can extract and evenly distribute it into a container placed on the circular plate at one time, reduces the operation steps, reduces the process of generating bacteria, and thus ensures the accuracy of the experimental data, can absorb culture fluid in batches and is suitable for matrix-arranged centralized culture sampling.

[0004] However, the above-mentioned device does not require manual assistance and can extract and distribute evenly into containers placed on the circular plate at one time, reducing the operation steps, and can be used for batch aspiration of culture fluid and is suitable for matrix-arranged centralized culture sampling. However, the device is applicable to fewer container specifications and cannot be applied to containers with large model differences.

[0005] Therefore, it is necessary to provide a new automatic sampling device for cell culture fluid to solve the above technical problems. Utility Model Content

[0006] In order to solve the above technical problems, the utility model provides an automatic sampling device for cell culture fluid.

[0007] The cell culture fluid automatic sampling device provided by the utility model comprises: a bottom plate, a sampling component, a sample placement component and a mobile body, the sampling component is movably connected to one side of the mobile body, the sample placement component is located below the sampling component, the sample placement component is movably connected to one side of the mobile body, and the sampling component, the sample placement component and the mobile body are all placed above the bottom plate;

[0008] The sampling assembly includes multiple sliders, multiple fixed blocks and multiple sampling tubes. The multiple sliders are slidably installed on the mobile body, and the multiple sampling tubes are installed on the multiple fixed blocks. The multiple sliders and the multiple fixed blocks move synchronously through an equidistant dispersion mechanism.

[0009] Preferably, the mobile body includes a mobile plate and a power device for driving the mobile plate to move laterally, a key slot is provided on one side of the mobile plate, the key slot is movably connected to the power device, and a slide rail is provided on the other side of the mobile plate, the slide rail is movably connected to the plurality of sliders.

[0010] Preferably, the equidistant dispersion mechanism comprises a plurality of first connecting rods and a plurality of second connecting rods, and the plurality of first connecting rods and the plurality of second connecting rods are hinged end to end;

[0011] The first connecting rod and the second connecting rod are hinged at the center, the first connecting rod and the second connecting rod at the head end are respectively hinged to the slider and the fixed block at the head end, and a pull rod is provided on the outside of the fixed block at the head end, and the first connecting rod and the second connecting rod at the tail end are respectively hinged to the inner wall of the moving body.

[0012] Preferably, the sample placing assembly comprises a lifting member, a sample placing plate and a sample placing box, the lifting member is connected to the sample placing plate, and the sample placing box is placed above the sample placing plate.

[0013] Preferably, the lifting member includes a screw rod, a base and a rotating member, the rotating member is installed on the base, the base is fixedly connected to the bottom plate, the screw rod is connected to the rotating member, and the outer surface of the screw rod cooperates with the template thread.

[0014] Preferably, the rotating member includes a worm, a worm wheel and a turntable, the worm is movably mounted on the base, the worm is meshed with the worm wheel, the worm wheel is fixedly connected to the bottom of the lead screw, and one end of the worm is fixedly connected to the turntable.

[0015] Preferably, the pull rod includes a connecting plate and an electric cylinder, the connecting plate is fixedly connected to the fixed block, a circular hole is opened on the connecting plate, the electric cylinder passes through the circular hole and is fixedly connected to the electric cylinder, and the other end of the electric cylinder is fixedly connected to one side of the slide rail.

[0016] Compared with the related art, the cell culture fluid automatic sampling device provided by the utility model has the following beneficial effects:

[0017] The utility model provides an automatic sampling device for cell culture fluid. For the problem of matrix-arranged centralized culture sampling, the device can adjust the spacing of sampling tubes to be applicable to a wider range of container specifications by pulling a pull rod to obtain a plurality of first connecting rods and a plurality of second connecting rods, wherein the plurality of first connecting rods and the plurality of second connecting rods are hinged end to end; the first connecting rods are hinged at the center of the second connecting rods, and the first connecting rods and the second connecting rods at the head end are respectively hinged to a sliding block and a fixed block at the head end through an equidistant dispersion mechanism, thereby solving the problem that the prior art can only be applicable to a smaller number of containers and cannot be applicable to containers with a large difference in model. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 This is a schematic diagram of the overall structure of the automatic sampling device for cell culture fluid provided by the utility model;

[0019] Figure 2 A schematic diagram of the internal structure of the automatic sampling device for cell culture fluid provided by the utility model;

[0020] Figure 3 A schematic diagram of the connection structure between the sampling assembly and the movable plate provided by the utility model;

[0021] Figure 4 A schematic diagram of the exploded structure of the sampling assembly and the movable plate provided by the utility model;

[0022] Figure 5 A schematic diagram of the structure of the equidistant dispersion mechanism provided by the utility model;

[0023] Figure 6 A schematic diagram of the lifting member structure provided by the utility model;

[0024] Figure 7 This is a schematic diagram of the sampling structure provided by the utility model.

[0025] Numbers in the figure: 1, bottom plate; 2, slider; 3, fixed block; 4, sampling tube; 5, first connecting rod; 6, second connecting rod; 7, moving plate; 8, keyway; 9, slide rail; 10, power device; 11, sample plate; 12, sample box; 13, screw rod; 14, base; 15, worm gear; 16, worm; 17, turntable; 18, pull rod; 19, connecting plate; 20, electric cylinder. DETAILED DESCRIPTION

[0026] The utility model is further described below in conjunction with the accompanying drawings and implementation modes.

[0027] Please refer to Figure 1-Figure 7 ,in, Figure 1 A schematic structural diagram of a preferred embodiment of the automatic sampling device for cell culture fluid provided by the utility model; Figure 2A schematic diagram of the internal structure of the automatic sampling device for cell culture fluid provided by the utility model; Figure 3 A schematic diagram of the connection structure between the sampling assembly and the movable plate provided by the utility model; Figure 4 A schematic diagram of the exploded structure of the sampling assembly and the movable plate provided by the utility model; Figure 5 A schematic diagram of the structure of the equidistant dispersion mechanism provided by the utility model; Figure 6 A schematic diagram of the lifting member structure provided by the utility model; Figure 7 This is a schematic diagram of the sampling structure provided by the utility model.

[0028] In the specific implementation process, Figure 1-Figure 7 As shown, a cell culture fluid automatic sampling device comprises a bottom plate 1, a sampling assembly, a sample placement assembly and a mobile body, wherein the sampling assembly is movably connected to one side of the mobile body, the sample placement assembly is located below the sampling assembly, the sample placement assembly is movably connected to one side of the mobile body, and the sampling assembly, the sample placement assembly and the mobile body are all placed above the bottom plate 1;

[0029] The sampling assembly includes multiple sliders 2, multiple fixed blocks 3 and multiple sampling tubes 4. The multiple sliders 2 are slidably installed on the mobile body, and the multiple sampling tubes 4 are installed on the multiple fixed blocks 3. The multiple sliders 2 and the multiple fixed blocks 3 move synchronously through an equidistant dispersion mechanism.

[0030] The mobile body includes a mobile plate 7 and a power device 10 for driving the mobile plate 7 to move horizontally. A key slot 8 is provided on one side of the mobile plate 7, and the key slot 8 is movably connected to the power device 10. A slide rail 9 is provided on the other side of the mobile plate 7, and the slide rail 9 is movably connected to a plurality of sliders 2.

[0031] Specifically, the structure of the power device 10 includes a motor, a gear and a rack. The rack is fixed on the base plate 1, and the gear is above the rack. The gear and the rack are meshed. The output end of the motor is fixedly connected to one side of the gear. The operation of the motor drives the gear to move. The motor and the gear move horizontally on the rack together. The other end of the motor is fixedly connected to the movable plate 7 to drive the movable plate 7 to move.

[0032] The equidistant dispersion mechanism includes a plurality of first connecting rods 5 and a plurality of second connecting rods 6, and the plurality of first connecting rods 5 and the plurality of second connecting rods 6 are hinged end to end; the first connecting rod 5 and the second connecting rod 6 are hinged at the center, the first connecting rod 5 and the second connecting rod 6 located at the head end are respectively hinged to the slider 2 and the fixed block 3 located at the head end, and a pull rod is provided on the outside of the fixed block 3 located at the head end, the pull rod 18 includes a connecting plate 19 and an electric cylinder 20, the connecting plate 19 is fixedly connected to the fixed block 3, a circular hole is provided on the connecting plate 19, the electric cylinder 20 is fixedly connected to the connecting plate 19 through the circular hole, and the other end of the electric cylinder 20 is fixedly connected to one side of the slide rail 9, the first connecting rod 5 and the second connecting rod 6 located at the tail end are respectively hinged to the inner side wall of the moving body, the hinge referred to here can be understood as being hinged to the block set in the slide rail 9, the connecting plate 19 and the fixed block 3 are driven to move by the extension and retraction of the electric cylinder 20, and the adjustment of the spacing between the plurality of sampling tubes 4 is achieved through the equidistant dispersion mechanism.

[0033] The sample placement assembly includes a lifting member, a sample placement plate 11 and a sample placement box 12. The lifting member is connected to the sample placement plate 11. The sample placement box 12 is placed above the sample placement plate 11. The sample placement box 12 can be replaced with an adaptive size according to the amount of culture fluid. The lifting member includes a screw rod 13, a base 14 and a rotating member. The rotating member is installed on the base 14. The base 14 is fixedly connected to the bottom plate 1. The screw rod 13 is connected to the rotating member. The outer surface of the screw rod 13 is threadedly matched with the sample placement plate 11. The rotating parts include a worm 16, a worm wheel 15 and a turntable 17. The worm 16 is movably mounted on the base 14. The worm 16 is meshed with the worm wheel 15. The worm wheel 15 is fixedly connected to the bottom of the lead screw 13. One end of the worm 16 is fixedly connected to the turntable 17. Rotating the turntable 17 can drive the worm 16 to rotate. The worm 16 is meshed with the worm wheel 15, and the worm wheel 15 rotates, driving the lead screw 13 in the middle of the worm wheel 15 to rotate. Finally, the sample plate 11 connected to the lead screw 13 through the thread moves in the vertical direction. The height adjustment of the sample plate 11 and the size change of the sample box 12 work together to achieve the purpose of easier and more standardized sampling.

[0034] The working principle provided by the utility model is as follows: during use, the motor moves horizontally on the rack, and the movement of the movable plate 7 fixedly connected to the motor drives the sampling tube 4 to move horizontally to complete the sampling. During the sampling operation, there may be a situation where there is less culture fluid. In this case, a smaller sample box 12 can be replaced, and then the worm gear 15 can be driven to move by rotating the plate, and the screw rod 13 can be rotated to adjust the sample plate 11 to an appropriate height for sampling. At the same time, the pull rod on the side of the lamp dispersion mechanism can also be pulled to reduce the spacing of the sampling tubes 4 to adapt to the sampling box for sampling. After the culture fluid is absorbed, when releasing liquid to containers of different models, the spacing of the sampling tubes 4 can be adjusted to be suitable for containers with large size differences.

[0035] The circuits and controls involved in the present invention are all prior art and will not be described in detail here.

[0036] The above are only embodiments of the present invention, and are not intended to limit the patent scope of the present invention. Any equivalent structure or equivalent process transformation made using the contents of the specification and drawings of the present invention, or directly or indirectly applied in other related technical fields, are also included in the patent protection scope of the present invention.

Claims

1. A cell culture fluid automatic sampling device, characterized in that: include: A bottom plate (1), a sampling assembly, a sample placement assembly, and a mobile body, wherein the sampling assembly is movably connected to one side of the mobile body, the sample placement assembly is located below the sampling assembly, the sample placement assembly is movably connected to one side of the mobile body, and the sampling assembly, the sample placement assembly, and the mobile body are all placed above the bottom plate (1); The sampling assembly comprises a plurality of sliders (2), a plurality of fixed blocks (3) and a plurality of sampling tubes (4); the plurality of sliders (2) are respectively slidably mounted on a mobile body; the plurality of sampling tubes (4) are respectively mounted on the plurality of fixed blocks (3); and the plurality of sliders (2) and the plurality of fixed blocks (3) move synchronously via an equidistant dispersion mechanism.

2. The automatic sampling device for cell culture fluid according to claim 1, characterized in that: The moving body comprises a moving plate (7) and a power device (10) for driving the moving plate (7) to move laterally; a key slot (8) is provided on one side of the moving plate (7); the key slot (8) is movably connected to the power device (10); a slide rail (9) is provided on the other side of the moving plate (7); the slide rail (9) is movably connected to the plurality of slide blocks (2).

3. The automatic sampling device for cell culture fluid according to claim 2, characterized in that: The equidistant dispersion mechanism comprises a plurality of first connecting rods (5) and a plurality of second connecting rods (6), wherein the plurality of first connecting rods (5) and the plurality of second connecting rods (6) are hingedly connected end to end; The first connecting rod (5) and the second connecting rod (6) are hinged at their centers; the first connecting rod (5) and the second connecting rod (6) at the head end are respectively hinged to the slider (2) and the fixed block (3) at the head end; a pull rod (18) is provided on the outside of the fixed block (3) at the head end; and the first connecting rod (5) and the second connecting rod (6) at the tail end are respectively hinged to the inner wall of the moving body.

4. The automatic sampling device for cell culture fluid according to claim 3, characterized in that: The sample placement assembly comprises a lifting member, a sample placement plate (11) and a sample placement box (12); the lifting member is connected to the sample placement plate (11), and the sample placement box (12) is placed above the sample placement plate (11).

5. The automatic sampling device for cell culture fluid according to claim 4, characterized in that: The lifting member comprises a screw rod (13), a base (14) and a rotating member, wherein the rotating member is mounted on the base (14), the base (14) is fixedly connected to the bottom plate (1), the screw rod (13) is connected to the rotating member, and the outer surface of the screw rod (13) is threadedly matched with the sample plate (11).

6. The automatic sampling device for cell culture fluid according to claim 5, characterized in that: The rotating member comprises a worm (16), a worm wheel (15) and a rotating disk (17); the worm (16) is movably mounted on the base (14); the worm (16) is meshed with the worm wheel (15); the worm wheel (15) is fixedly connected to the bottom of the lead screw (13); and one end of the worm (16) is fixedly connected to the rotating disk (17).

7. The automatic sampling device for cell culture fluid according to claim 6, characterized in that: The pull rod (18) comprises a connecting plate (19) and an electric cylinder (20), wherein the connecting plate (19) is fixedly connected to the fixing block (3), a circular hole is formed on the connecting plate (19), the electric cylinder (20) passes through the circular hole and is fixedly connected to the connecting plate (19), and the other end of the electric cylinder (20) is fixedly connected to one side of the slide rail (9).

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