Biosafety experimental device for breeding mosquitoes and other medical arthropods infected with mosquitoes and other medical arthropods

Abstract

The utility model discloses a biosafety type experimental device used for infection breeding of mosquitoes and other medical arthropods, the biosafety type experimental device comprises an infection breeding container and an anesthesia container, the infection breeding container comprises a first cylindrical bottle body and a gauze threaded cover matched with the first cylindrical bottle body, the top of the gauze threaded cover is made of a gauze material; the infection feeding container is used for feeding and infecting mosquitoes and other medical arthropods, on one hand, the mosquitoes and other medical arthropods can be effectively prevented from escaping when being subjected to artificial blood meal or animal living body blood feeding infection, and on the other hand, the blood meal infection process can be conveniently observed in real time; the blood meal state and the survival condition of the medical arthropods in the feeding process are determined; six small holes with the diameter of 3 mm are formed in a round snap-fastener plastic cover of the anesthesia container, gas is prevented from directly impacting medical arthropods, carbon dioxide is prevented from being dissipated too fast, and therefore mosquitoes are anesthetized efficiently and mildly.

Description

Technical Field

[0001] The utility model belongs to the technical field of biological safety devices, and particularly relates to a biological safety experimental device used for infection and breeding of mosquitoes and other medical arthropods. Background Art

[0002] In mosquito and other medical arthropod pathogen infection experiments, a suitable container for mosquito and other medical arthropod infection experiments, including artificial blood meals and live animal blood feeding, and feeding is essential. The container must first ensure its tightness and firmness to effectively prevent the escape of arthropods. It must then be convenient for arthropods to suck blood or sugar water, and provide the operator with an environment that is convenient for observation, thereby preventing the escape of arthropods during the experiment and ensuring the convenience and biosafety of the experimental operation process.

[0003] At present, there is no professional experimental container for mosquito and other medical arthropod infection and breeding on the market. The experimental containers used in the laboratories of relevant institutions have no unified standards and have the following problems: (1) Most of them are simply assembled in the laboratory, and the gauze and the container body are not tightly and firmly closed, and the operation is inconvenient, especially when the container is opened and closed, and when accidents such as container tipping occur, the risk of arthropod escape increases; (2) It is difficult to observe the state of arthropods in real time in the container, which is not conducive to researchers to judge whether the blood meal is sufficient and the survival status; (3) Some are made of paper materials. Once the pathogens contaminate the surface of the container during the experiment, it is not easy to disinfect and decontaminate. In recent years, research on insect-borne pathogens has received more and more attention. Therefore, there is an urgent need for a safe and easy-to-operate experimental container to meet the relevant scientific research needs, so as to ensure the smooth progress of experiments such as mosquito and other medical arthropod infection and breeding and reduce the biosafety risk of arthropod escape in the experiment.

[0004] In addition, in mosquito and other medical arthropod infection tests, if carbon dioxide gas is used to anesthetize arthropods, the strong impact of the airflow from the carbon dioxide anesthesia gun will damage the abdominal wall of the blood-saturated arthropods, resulting in death, which will reduce the quality of the test and increase the risk of pathogen exposure. Therefore, equipped with professional anesthesia containers can achieve the purpose of efficient, safe and gentle anesthesia of arthropods. Utility Model Content

[0005] The utility model provides a biosafety experimental device for the infection and breeding of mosquitoes and other medical arthropods, which is a container for infecting and breeding mosquitoes and other medical arthropods and effectively preventing them from escaping, and a matching carbon dioxide anesthesia container. The container has the advantages of preventing medical arthropods with flying ability from escaping and being easy to use, and solves four problems existing in existing devices: (1) There is no unified standard for the infection and breeding containers for mosquitoes and other medical arthropods at present, and most of them are simple and homemade. The connection between the gauze and the container body is not tight and firm enough, and arthropods are easy to escape during the opening and closing process of the container, and when the container is tipped over or other accidents occur; (2) It is difficult to observe the state of arthropods in real time in the container, which is not conducive to researchers to judge the blood meal sucking situation and the survival state; (3) Some of them are made of paper materials, which are not strong enough. Once the pathogens contaminate the surface of the container during the experiment, the disinfection and decontamination effect is not good; (4) There is currently no container suitable for carbon dioxide anesthesia that is matched with the infection container, and mosquitoes and other medical arthropods cannot be anesthetized efficiently, safely and gently.

[0006] To solve the above problems, the technical solutions provided by the present invention are as follows:

[0007] The utility model embodiment provides a biosafety experimental device for infection and breeding of mosquitoes and other medical arthropods, which includes an infection breeding container and an anesthesia container. The infection breeding container is used for infecting and breeding mosquitoes and other medical arthropods and effectively preventing their escape; the anesthesia container is used for efficient and gentle anesthesia of mosquitoes and other medical arthropods in the container using a carbon dioxide method.

[0008] The infection breeding container includes a first cylindrical bottle body and a gauze threaded cover matched with the first cylindrical bottle body, the gauze threaded cover includes an outer bottom with an internal thread and an upper gauze, the edge of the gauze is integrally connected with the outer bottom, the internal thread of the outer bottom and the external thread of the end of the first cylindrical bottle body are screwed together; the mesh number of the gauze is selected according to the individual size of different types of medical arthropods, for example, when used for mosquito infection breeding, the gauze size is selected to be 16 to 40 meshes.

[0009] The anesthesia container includes a second transparent cylindrical bottle body and a circular snap-on plastic cover matching the second transparent cylindrical bottle body, the circular snap-on plastic cover is provided with 6 small holes with a diameter of 3 mm, and the concave step structure on the edge of the circular snap-on plastic cover is interlocked with the protruding step structure of the second transparent cylindrical bottle body.

[0010] According to an optional embodiment of the present invention, the first cylindrical bottle body and the second transparent cylindrical bottle body are both made of transparent plastic material.

[0011] According to an optional embodiment of the utility model, the material of the circular snap button plastic cover is transparent plastic.

[0012] Beneficial effects: The embodiment of the utility model provides a biosafety experimental device for infection and breeding of mosquitoes and other medical arthropods, including an infection breeding container and an anesthesia container. The infection breeding container is used for infecting and breeding mosquitoes and other medical arthropods. It is easy to use and effectively prevents their escape, and the arthropods therein can be observed in real time to determine their blood meal status and survival status during the breeding process; the anesthesia container is used for carbon dioxide anesthesia of mosquitoes and other medical arthropods to achieve an efficient and mild anesthetic effect.

[0013] The upper part of the gauze threaded cover of the infection breeding container is made of gauze material, and the mesh number of the gauze is selected according to the individual size of different types of medical arthropods, and mosquitoes generally choose 16-40 mesh. Mosquitoes and other medical arthropods can directly suck blood meals and sugar water or bite animals through the gauze, and the mesh aperture is smaller than the volume of mosquitoes and other medical arthropods, avoiding the occurrence of escape. The gauze threaded cover is an integrated design, and is screwed together with the external thread at the end of the first cylindrical bottle body. The lid is easy to open and close, further reducing the risk of potential infected mosquitoes and other medical arthropods escaping due to the infection breeding container not being closed tightly or accidentally opening due to collision. The main body of the infection breeding container is made of plastic, which is conducive to the thorough decontamination and disinfection of the container surface by the disinfectant, reducing the risk of pathogen contamination. In addition, the infection breeding container is a transparent structure, and the state of the arthropods in the infection breeding container can be observed in 360 degrees in all directions, so as to judge the blood meal and its survival.

[0014] The anesthesia container for carbon dioxide anesthesia of the utility model is made of transparent plastic material, and can observe the anesthesia conditions of mosquitoes and other medical arthropods in real time in all directions without blind spots, and at the same time slow down the decline of carbon dioxide concentration, improve the anesthesia efficiency, and the circular snap-on plastic cover is provided with 6 small holes with a diameter of 3mm, which can prevent the anesthesia container from bursting due to excessive air pressure inside the anesthesia container. In addition, the carbon dioxide airflow will not be sprayed directly onto the body surface of the arthropod, reducing the damage to it caused by excessive airflow. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] In order to more clearly illustrate the technical solutions in the embodiments or the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0016] Figure 1A schematic diagram of the three-dimensional structure of an infection breeding container of a biosafety experimental device for infection breeding of mosquitoes and other medical arthropods provided in an embodiment of the present application.

[0017] Figure 2 A schematic diagram of the first cylindrical bottle structure of an infection breeding container of a biosafety experimental device for infection breeding of mosquitoes and other medical arthropods provided in an embodiment of the present application.

[0018] Figure 3 A schematic diagram of the structure of a mesh threaded cover for an infection breeding container of a biosafety experimental device for infection breeding of mosquitoes and other medical arthropods provided in an embodiment of the present application.

[0019] Figure 4 A schematic diagram of the three-dimensional structure of an anesthesia container of a biosafety experimental device for breeding mosquitoes and other medical arthropods for infection provided in an embodiment of the present application.

[0020] Figure 5 A schematic diagram of the second transparent cylindrical bottle structure of an anesthesia container of a biosafety experimental device for breeding mosquitoes and other medical arthropods for infection provided in an embodiment of the present application.

[0021] Figure 6 A schematic diagram of the structure of a circular snap-on plastic cover of an anesthesia container of a biosafety experimental device for breeding infection of mosquitoes and other medical arthropods provided in an embodiment of the present application. DETAILED DESCRIPTION

[0022] The technical solutions in the embodiments of the present application will be described clearly and completely below in conjunction with the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative work are within the scope of protection of the present application.

[0023] Figure 1 and Figure 3 As shown, the embodiment of the utility model provides a schematic diagram of the structure of a biosafety experimental device for infection and breeding of mosquitoes and other medical arthropods. The biosafety experimental device includes an infection breeding container and an anesthesia container. The infection breeding container is used for infecting and breeding mosquitoes and other medical arthropods. It is convenient to use and can effectively prevent them from escaping. The arthropods inside can be observed in real time to determine the blood meal situation and the survival situation during the breeding process; the anesthesia container is used in the process of anesthetizing mosquitoes and other medical arthropods with carbon dioxide to achieve an efficient and mild anesthesia effect.

[0024] like Figure 1 , Figure 2 and Figure 3 As shown, the infection breeding container includes a first cylindrical bottle body 1 and a gauze threaded cover matched with the first cylindrical bottle body, the gauze threaded cover includes an outer bottom 2 with internal threads and a gauze 3 on the upper part, and the outer bottom 2 and the first cylindrical bottle body 1 are both transparent parts. The edge of the gauze 3 is integrally connected with the outer bottom 2; the mesh number of the gauze is selected according to the size of different types of medical arthropods, for example, the size of the mosquito gauze is generally 16 to 40 meshes.

[0025] The inner thread of the outer bottom 2 is screwed together with the outer thread 1-1 at the end of the first cylindrical bottle body 1 to ensure that the mesh threaded cover can be firmly fixed on the first cylindrical bottle body 1. That is, the spiral opening on the outer surface of the upper end of the first cylindrical bottle body 1 is consistent with the spiral opening of the outer bottom 2 of the mesh threaded cover.

[0026] In this embodiment, by setting the top of the cylindrical transparent screw cap as a gauze, mosquitoes and other medical arthropods can directly suck blood meals and sugar water or bite animals through the gauze, and the mesh aperture is smaller than the volume of the individual arthropods, avoiding the occurrence of escape. The gauze 3 is connected with the outer bottom 2 in an integrated manner, and the gauze threaded cover is closed with the first cylindrical bottle body 1 of transparent plastic material by screwing it tightly, and the opening and closing operations are convenient, further reducing the risk of mosquitoes and other medical arthropods escaping when the infected container is not closed tightly or accidentally opened. The plastic material can facilitate the thorough decontamination and disinfection of the container surface by the disinfectant, reducing the risk of pathogen contamination. The container body is a transparent component, and the arthropods in the container can be observed 360 degrees in all directions to determine their blood meals and survival conditions.

[0027] like Figure 4 , Figure 5 and Figure 6 As shown, the anesthesia container includes a second transparent cylindrical bottle body 4 and a circular snap-on plastic cover 5 matched with the second transparent cylindrical bottle body 4, and the circular snap-on plastic cover 5 has 6 small holes 6 with a diameter of 3 mm, and the concave step structure on the edge of the circular snap-on plastic cover 5 is interlocked with the protruding step structure of the second transparent cylindrical bottle body 4. The circular snap-on plastic cover 5 is made of transparent plastic material, which can observe the anesthesia of mosquitoes and other medical arthropods in real time without blind spots in all directions, and at the same time slow down the decline of carbon dioxide concentration, improve the anesthesia efficiency, and the circular snap-on plastic cover 5 has 6 small holes 6 with a diameter of 3 mm, which can avoid the occurrence of bursting due to excessive air pressure inside the anesthesia container. In addition, the carbon dioxide airflow will not be directly sprayed onto the arthropod body surface, reducing the damage to the arthropod caused by excessive airflow. The anesthesia container in this embodiment is a snap-on semi-enclosed container, which slows down the decline of carbon dioxide concentration, improves the anesthesia efficiency, and the airflow will not be directly sprayed onto the arthropod body surface, reducing the damage to the arthropod caused by excessive airflow.

[0028] The first cylindrical bottle body 1 and the second transparent cylindrical bottle body 4 are preferably made of transparent plastic material.

[0029] When in use, the infected breeding container containing mosquitoes or other medical arthropods is transferred to a glove box or a biosafety cabinet, and the module containing the infectious blood meal is placed on the gauze 3 for feeding through an artificial blood meal device in the glove box, or the gauze 3 of the infected breeding container containing mosquitoes or other medical arthropods is made to be close to the animal body surface in the biosafety cabinet for live blood feeding. After the blood meal is completed, the above-mentioned infected container containing infected mosquitoes or other medical arthropods is placed in the glove box. When sorting blood-saturated mosquitoes or other medical arthropods, first the infected breeding container is placed in a matching carbon dioxide anesthesia container, an anesthesia gun head is inserted from the edge small hole 6, the carbon dioxide valve is opened to start anesthesia, the anesthesia process can be observed in real time through the transparent bottle body, about 30 seconds later, the anesthetized mosquitoes or other medical arthropods are transferred to the ice row or freezing table in the glove box for sorting, the sorted mosquitoes or other medical arthropods are transferred to a new infected breeding container, affixed with a label, and then transferred to an incubator for breeding.

[0030] In summary, although the present invention has been disclosed as above in terms of preferred embodiments, the above preferred embodiments are not intended to limit the present invention. A person skilled in the art may make various changes and modifications without departing from the spirit and scope of the present invention. Therefore, the scope of protection of the present invention shall be based on the scope defined in the claims.

Claims

1. A biosafety experimental device for infection and breeding of mosquitoes and other medical arthropods, characterized in that: It includes an infection breeding container and an anesthesia container. The infection breeding container is used for blood meal infection and breeding of mosquitoes and other medical arthropods, and effectively prevents their escape; the anesthesia container is used for efficient and gentle anesthesia of mosquitoes and other medical arthropods in the infection breeding container by carbon dioxide method; The infection breeding container comprises a first cylindrical bottle body and a gauze threaded cover matched with the first cylindrical bottle body, wherein the gauze threaded cover comprises an outer bottom with an internal thread and an upper gauze, wherein the edge of the gauze is integrally connected with the outer bottom, and the internal thread of the outer bottom is screwed with the external thread of the end of the first cylindrical bottle body; the mesh number of the gauze is selected according to the individual size of different medical arthropods, and when used for mosquito infection breeding, the mesh size is selected to be 16 to 40 meshes; The anesthesia container includes a second transparent cylindrical bottle body and a circular snap-on plastic cover matching the second transparent cylindrical bottle body, the circular snap-on plastic cover is provided with 6 small holes with a diameter of 3 mm, and the concave step structure on the edge of the circular snap-on plastic cover is interlocked with the protruding step structure of the second transparent cylindrical bottle body.

2. A biosafety experimental device for infection and breeding of mosquitoes and other medical arthropods according to claim 1, characterized in that: The first cylindrical bottle body and the second transparent cylindrical bottle body are both made of transparent plastic material.

3. A biosafety experimental device for infection and breeding of mosquitoes and other medical arthropods according to claim 1, characterized in that: The material of the circular snap button plastic cover is transparent plastic.

Images