Cell factor ultrafiltration device

Abstract

The utility model relates to the technical field of cell ultrafiltration, and discloses a cell factor ultrafiltration device which comprises a box body, a rough filtration membrane and an ultrafiltration membrane, the rough filtration membrane and the ultrafiltration membrane are respectively arranged at the upper end and the lower end in the box body, and the rough filtration membrane and the ultrafiltration membrane are respectively and fixedly arranged on a fixing frame and a fixing plate which are arranged in the box body in a sliding mode. A plurality of rough filtration frames are arranged on the fixed frame and located above the rough filtration membrane, a feeding groove is connected to the upper end of the box body, a plurality of dispersion rods are connected to the position, located below the feeding groove, in the box body, a first guide hopper and a second guide hopper are connected to the positions, located on the upper side and the lower side of the ultrafiltration membrane, in the box body respectively, and a collection box is connected to the position below the box body. The utility model has the advantages that the ultrafiltration membrane is convenient to replace, the weight is uniform in the filtering stage, and the filtering effect is better.

Description

Technical Field

[0001] The utility model relates to the technical field of cell ultrafiltration, in particular to a cytokine ultrafiltration device. Background Art

[0002] Cytokines are a class of secreted proteins that play a key regulatory role in intercellular communication. These proteins are able to transmit signals between cells and regulate biological processes such as immune response, cell proliferation, differentiation and apoptosis. Cytokines play an important role in the immune system, inflammatory response, tissue repair and regeneration. Ultrafiltration of cytokines is a common experimental technique, which is often used to isolate and concentrate specific cytokines. Ultrafiltration separates large molecules from small molecules in a solution by separating the size of the molecules. In this process, a selective filter membrane allows substances smaller than a certain molecular size to pass through, while larger molecules are blocked. Therefore, ultrafiltration can be used to separate cytokines from a mixed solution and can be concentrated for subsequent laboratory analysis or application.

[0003] Current cytokine ultrafiltration devices have some inconveniences in replacing the coarse filtration membrane and the ultrafiltration membrane. Usually, both membranes are located inside the housing of the device, so it is troublesome to replace them. If these membranes are not replaced for a long time, the filtration effect will decrease. In addition, if the filtrate accumulates too much in the device, it will cause great pressure on the center of the membrane, and it will not be possible to achieve a good uniform filtration effect, especially in the coarse filtration stage because of the heavy weight at this time. Utility Model Content

[0004] In order to solve the above-mentioned problems, the utility model provides a cytokine ultrafiltration device which is easy to replace the ultrafiltration membrane, has uniform weight in the filtering stage and has better filtering effect.

[0005] In order to solve the above-mentioned technical problems, the technical solution proposed by the utility model is: a cytokine ultrafiltration device, comprising a casing, a coarse filtration membrane and an ultrafiltration membrane, the coarse filtration membrane and the ultrafiltration membrane are respectively arranged at the upper and lower ends of the casing, the coarse filtration membrane and the ultrafiltration membrane are respectively fixed on a fixed frame and a fixed plate slidably arranged in the casing, a plurality of coarse filtration frames are arranged on the fixed frame above the coarse filtration membrane, a feed trough is connected to the upper end of the casing, a plurality of dispersion rods are connected to the lower part of the feeding trough in the casing, a guide bucket 1 and a guide bucket 2 are respectively connected to the upper and lower sides of the ultrafiltration membrane in the casing, and a collection box is connected to the lower part of the casing.

[0006] Furthermore, a handle is provided on one side of the fixing frame and the fixing plate extending out of the box body for connection.

[0007] Based on the above features, it is convenient to move the fixing frame and the fixing plate, thereby facilitating the replacement of the coarse filtration membrane and the ultrafiltration membrane.

[0008] Furthermore, a limiting slide matching the fixing frame and the fixing plate is provided on the inner side wall of the box body.

[0009] Based on the above features, the limiting slide can ensure that the fixing frame and the fixing plate can smoothly enter and be stably fixed in the box, thereby ensuring the stability of the coarse filtration membrane and the ultrafiltration membrane.

[0010] Furthermore, the coarse filter frame is divided by a plurality of evenly distributed horizontal plates and vertical plates fixed on the inner side of the fixed frame.

[0011] Based on the above characteristics, the evenly distributed horizontal plates and vertical plates separate the top of the coarse filter membrane into a uniform coarse filter frame, which facilitates the uniform distribution of the fluid on the coarse filter membrane, thereby ensuring uniform force thereon.

[0012] Furthermore, the feed trough is semi-cylindrical and two are symmetrically arranged, and the discharge port of the feed trough is arranged at the lowest point of its bottom surface.

[0013] Based on the above characteristics, the feed trough can not only play a good guiding role for the fluid, but also prevent the fluid from remaining in the feed trough to avoid waste.

[0014] Furthermore, the vertical cross-section of the dispersion rod is a triangle, and the dispersion rods are distributed in a tower shape up and down.

[0015] Based on the above characteristics, the fluid can be effectively and evenly dispersed.

[0016] Furthermore, a plurality of dispersion buckets are evenly arranged below the first guide bucket, and a guide pipe is connected to the second guide bucket below.

[0017] Based on the above characteristics, multiple dispersion buckets can ensure that the fluid is evenly dispersed above the ultrafiltration membrane, which is beneficial to improving the ultrafiltration efficiency. The guide tube can ensure that the high-concentration cytokine fluid after ultrafiltration enters the collection box smoothly to avoid falling into the box and causing waste.

[0018] Furthermore, the collection box is detachable.

[0019] Based on the above characteristics, it is convenient to dismantle and process the fluid after ultrafiltration.

[0020] Compared with the prior art, the utility model has the following advantages: the coarse filtration membrane and the ultrafiltration membrane are fixed by a fixed frame and a fixed plate slidably installed inside the box body, which is convenient for being directly taken out of the box body for replacement, thereby ensuring a good filtering effect; the dispersion rods with a triangular vertical cross-section and tower-shaped distribution up and down can effectively disperse the feed, ensuring the uniform distribution of the fluid on the surface of the filter membrane, which can not only reduce the weight of the middle part of the coarse filtration membrane, but also improve the filtration efficiency; at the same time, the design of the two feed troughs is also convenient for the fluid to be dispersed and fall; the design of multiple dispersion buckets makes it convenient for the fluid after coarse filtration to fall evenly on the ultrafiltration membrane, and can also make the pressure distribution of the ultrafiltration membrane uniform, thereby ensuring its effective filtration and improving the ultrafiltration efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 It is a three-dimensional diagram of the utility model;

[0022] Figure 2 It is a cross-sectional view of the utility model;

[0023] Figure 3 It is a three-dimensional diagram of the fixed frame part of the utility model;

[0024] Figure 4 It is a stereogram of the fixed plate part of the utility model.

[0025] As shown in the figure: 1. Box body; 2. Coarse filtration membrane; 3. Ultrafiltration membrane; 4. Fixed frame; 5. Fixed plate; 6. Feed trough; 7. Dispersion rod; 8. Guide bucket one; 9. Guide bucket two; 10. Collection box; 11. Handle; 12. Limit slide; 13. Horizontal plate; 14. Vertical plate; 15. Dispersion bucket; 16. Guide tube. DETAILED DESCRIPTION

[0026] The utility model is further described in detail below in conjunction with the accompanying drawings.

[0027] Combined with Figure 2 , Attachment Figure 3 , Attachment Figure 4 A cytokine ultrafiltration device comprises a housing 1, a coarse filtration membrane 2 and an ultrafiltration membrane 3, wherein the coarse filtration membrane 2 and the ultrafiltration membrane 3 are respectively arranged at the upper and lower ends of the housing 1, and the coarse filtration membrane 2 and the ultrafiltration membrane 3 are respectively fixed on a fixed frame 4 and a fixed plate 5 which are slidably arranged in the housing 1, and a limiting slide 12 matching the fixed frame 4 and the fixed plate 5 is arranged on the inner side wall of the housing 1, and the limiting slide 12 can ensure that the fixed frame 4 and the fixed plate 5 can smoothly enter and stably be fixed in the housing 1, thereby ensuring the stability of the coarse filtration membrane 2 and the ultrafiltration membrane 3. A handle 11 is provided on one side of the fixed frame 4 and the fixed plate 5 extending out of the housing 1, so as to facilitate the movement of the fixed frame 4 and the fixed plate 5, thereby facilitating the replacement of the coarse filtration membrane 2 and the ultrafiltration membrane 3.

[0028] Combined with Figure 3 A plurality of coarse filter frames are provided on the fixed frame 4 above the coarse filter membrane 2. The coarse filter frames are divided by a plurality of evenly distributed transverse plates 13 and vertical plates 14 fixed on the inner side of the fixed frame 4. The evenly distributed transverse plates 13 and vertical plates 14 separate the coarse filter membrane 2 into uniform coarse filter frames, which facilitates the uniform distribution of the fluid on the coarse filter membrane 2, thereby ensuring uniform force thereon.

[0029] Combined with Figure 1 , Attachment Figure 2The upper end of the box body 1 is connected to a feed trough 6, which is semi-cylindrical and symmetrically provided with two feed troughs. The discharge port of the feed trough 6 is located at the lowest point of its bottom surface. The feed trough 6 can not only play a good guiding role for the fluid, but also prevent the fluid from remaining in the feed trough 6 to avoid waste.

[0030] Combined with Figure 2 A plurality of dispersion rods 7 are connected to and provided below the feed trough 6 in the box body 1. The vertical cross-section of the dispersion rods 7 is triangular. The dispersion rods 7 are distributed in a tower shape up and down, which can effectively and evenly disperse the fluid.

[0031] Combined with Figure 2 In the box body 1, a guide bucket 1 8 and a guide bucket 2 9 are respectively connected to the upper and lower sides of the ultrafiltration membrane 3. A plurality of dispersion buckets 15 are evenly arranged below the guide bucket 1 8. A guide tube 16 is connected below the guide bucket 2 9. The plurality of dispersion buckets 15 can ensure that the fluid is evenly dispersed above the ultrafiltration membrane 3, which is beneficial to improving the ultrafiltration efficiency. The guide tube 16 can ensure that the high-concentration cytokine fluid after ultrafiltration enters the collection box 10 smoothly to avoid falling into the box body 1 and causing waste.

[0032] Combined with Figure 1 , Attachment Figure 2 A collecting box 10 is connected to the lower part of the box body 1. The collecting box 10 is detachable, which is convenient for removing and processing the fluid after ultrafiltration.

[0033] The specific implementation mode of the utility model is as follows: the box body 1 is stably placed in a suitable position, a clean coarse filtration membrane 2 and an ultrafiltration membrane 3 are placed in the box body 1, the fixed frame 4 and the fixed plate 5 are made to slide into the limit slide 12, and the fluid to be filtered is added into the feed trough 6. The fluid flows out through the discharge port and is dispersed and falls into the coarse filtration frame on the coarse filtration membrane 2 under the action of the dispersion rod 7. After coarse filtration by the coarse filtration membrane 2, the fluid enters the guide bucket 9, and under the action of the guide bucket 9, it is dispersed through each dispersion bucket 15 and falls on the ultrafiltration membrane 3, and then falls into the guide bucket 9 after being filtered by the ultrafiltration membrane 3. Finally, the high-concentration cytokine fluid falls into the collection box 10 through the guide tube 16, and the collection box 10 is removed to collect and process the fluid after ultrafiltration, and the fixed frame 4 and the fixed plate 5 are taken out regularly to replace the coarse filtration membrane 2 and the ultrafiltration membrane 3.

[0034] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the term "connection" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection. For ordinary technicians in this field, the specific meaning of the above terms in the present invention can be understood according to specific circumstances.

[0035] The above description of the utility model and its implementation methods is not restrictive. The drawings show only one implementation method of the utility model, and the actual structure is not limited thereto. In short, if ordinary technicians in this field are inspired by it and design structural methods and embodiments similar to the technical solution without creativity without departing from the purpose of the invention of the utility model, they should all fall within the protection scope of the utility model.

Claims

1. A cytokine ultrafiltration device, comprising a housing (1), a coarse filtration membrane (2) and an ultrafiltration membrane (3), characterized in that: The coarse filtration membrane (2) and the ultrafiltration membrane (3) are respectively arranged at the upper and lower ends of the box body (1); the coarse filtration membrane (2) and the ultrafiltration membrane (3) are respectively fixed on a fixed frame (4) and a fixed plate (5) slidably arranged in the box body (1); a plurality of coarse filtration frames are arranged on the fixed frame (4) above the coarse filtration membrane (2); a feed trough (6) is connected to the upper end of the box body (1); a plurality of dispersion rods (7) are connected to the lower part of the feed trough (6) in the box body (1); a guide bucket 1 (8) and a guide bucket 2 (9) are respectively connected to the upper and lower sides of the ultrafiltration membrane (3) in the box body (1); and a collection box (10) is connected to the lower part of the box body (1).

2. A cytokine ultrafiltration device according to claim 1, characterized in that: One side of the fixing frame (4) and the fixing plate (5) extends out of the box body (1) and is connected to a handle (11).

3. A cytokine ultrafiltration device according to claim 1, characterized in that: The inner side wall of the box body (1) is provided with a limiting slideway (12) matching the fixing frame (4) and the fixing plate (5).

4. A cytokine ultrafiltration device according to claim 1, characterized in that: The coarse filter frame is divided by a plurality of evenly distributed horizontal plates (13) and vertical plates (14) fixed on the inner side of the fixed frame (4).

5. A cytokine ultrafiltration device according to claim 1, characterized in that: The feed trough (6) is semi-cylindrical and two are symmetrically arranged, and the discharge port of the feed trough (6) is arranged at the lowest point of its bottom surface.

6. A cytokine ultrafiltration device according to claim 1, characterized in that: The dispersion rod (7) has a triangular vertical cross-section, and the dispersion rods (7) are distributed in a tower shape up and down.

7. A cytokine ultrafiltration device according to claim 1, characterized in that: A plurality of dispersion buckets (15) are evenly arranged below the first guide bucket (8), and a guide pipe (16) is connected to the bottom of the second guide bucket (9).

8. A cytokine ultrafiltration device according to claim 1, characterized in that: The collecting box (10) is detachable.

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