Multi-channel dynamic pressurization explant in-vitro culture device

Abstract

The utility model discloses a multi-channel dynamic pressurization explant in-vitro culture device which comprises a control assembly, a pressure sensor and a pressure sensor, the covering assembly comprises a cover body, and the inner wall of the cover body is provided with a plurality of pressurizing assemblies corresponding to the hole bodies in the hole plate; each pressurizing assembly is electrically connected with the control assembly; each pressurizing assembly comprises a pressing block, a pressure sensor integrated on the bottom wall of the pressing block and an electric telescopic rod connected between the pressing block and the cover body. According to the multi-channel dynamic pressurization explant culture device disclosed by the utility model, the cover body with the plurality of groups of pressurization assemblies and the control assembly for controlling the dynamic pressurization of the cover body are arranged, so that each channel can be independently subjected to pressurization, decompression and constant-pressure operation; the channels can be used for simultaneously carrying out a plurality of explant mechanical stimulation experiments, so that the experiment efficiency is improved, and the experiment error is reduced.

Description

Technical Field

[0001] The utility model belongs to the technical field of mechanical stimulation culture of explants, and in particular relates to a multi-channel dynamic pressurized explant in vitro culture device. Background Art

[0002] It is well known that dynamic mechanical stimulation plays a key role in the development of bones during embryonic and postnatal development. In addition, the imbalance of force lines caused by external factors such as aging or trauma significantly accelerates and aggravates the degeneration of osteochondrocytes. The above phenomena show that mechanical force has an important regulatory role in the development, regeneration and degeneration of osteochondrocytes, but the specific regulatory pathways and mechanisms are unclear, and the current relevant intervention measures lack effectiveness verification in the mechanical microenvironment. In order to reveal the effects and mechanisms of mechanical signals on osteochondrocytes, evaluate the efficacy and safety of treatment strategies in the mechanical microenvironment, shorten the test cycle and reduce human errors, it is urgent to develop a multi-channel 3D loading device.

[0003] At present, it is generally recognized in academia that three-dimensional culture technology can better simulate the state of cells in the body, enhance cell interactions and matrix synthesis, so explant culture technology has attracted much attention. For example, in the skeletal system, cartilage caps are a common source of explants for studying mechanical regulation of cartilage phenotypes and targeted therapy. However, the cartilage caps from different individuals vary in size, and the commonly used loading devices are mostly single-channel pressurization (including direct physical pressurization with titanium blocks, or the use of air pressure, fluid pressure, etc.), lacking precise control of sample and sensor contact and consistent pressurization coordination, resulting in uneven force, inability to quantify pressure, and difficulty in reproducing conditions in different test batches. The resulting errors are enough to mask the true mechanical stimulation effect.

[0004] In summary, there is an urgent need to design a multi-channel dynamic pressurized explant in vitro culture device that overcomes the above-mentioned technical problems. Utility Model Content

[0005] In view of the problems mentioned in the background technology, the purpose of the present invention is to provide a multi-channel dynamic pressurized explant in vitro culture device to solve the problems mentioned in the background technology.

[0006] In order to achieve the above purpose, the utility model mainly provides the following technical solutions:

[0007] A multi-channel dynamic pressurized explant in vitro culture device, comprising:

[0008] Control components;

[0009] A cover assembly comprises a cover body, wherein the inner wall of the cover body is provided with a plurality of pressurizing assemblies corresponding to the holes of the orifice plate; each pressurizing assembly is electrically connected to the control assembly;

[0010] Each group of pressurizing components comprises a press block, a pressure sensor integrated at the bottom wall of the press block, and an electric telescopic rod connected between the press block and the cover body.

[0011] Furthermore, the control component includes a PCB circuit board, a microprocessor and a power supply component integrated on the PCB circuit board;

[0012] It also includes a touch display screen which is electrically connected to the microprocessor.

[0013] Furthermore, the cover body is provided with a plurality of openings, and an electrical interface is passed through each opening;

[0014] The opening is sealed with a flexible rubber gasket.

[0015] Furthermore, it also includes a cable, one end of which is connected to the electrical interface through each electrical plug, and the other end is plugged into the interface end on the control component.

[0016] Furthermore, the pressing block is preferably made of medical stainless steel or polytetrafluoroethylene;

[0017] The pressure sensor is preferably a thin film pressure sensor.

[0018] Compared with the prior art, the beneficial effects of the utility model are mainly reflected in:

[0019] The multi-channel dynamic pressurized explant in vitro culture device of the utility model is provided with a cover body having multiple groups of pressurizing components and a control component for controlling its dynamic pressurization, so that each channel can independently perform pressurization, decompression and constant pressure operations; these channels can perform multiple explant mechanical stimulation experiments at the same time, thereby improving the efficiency of the experiment and reducing experimental errors.

[0020] Furthermore, the 3D pressurization device of this patent is not only suitable for pressurization of explants, but also has the same mechanical stimulation effect on organoids and bioactive materials;

[0021] Finally, by setting up pressure sensors and microprocessors, the magnitude and duration of pressure can be precisely adjusted; the 3D mechanical culture device is easy to operate, has adjustable pressure, and simulates the in vivo environment as much as possible.

[0022] The above description is only an overview of the technical solution of the utility model. In order to clearly understand the technical means of the utility model and implement it according to the contents of the specification, the following preferred embodiments of the utility model are described in detail with reference to the accompanying drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0024] Figure 1 It is a schematic diagram of the structure of the multi-channel dynamic pressurized explant in vitro culture device of the utility model;

[0025] Figure 2 It is a partial structural schematic diagram of the multi-channel dynamic pressurized explant in vitro culture device of the utility model;

[0026] Figure 3 for Figure 2 A magnified view of point A;

[0027] Figure 4 A schematic circuit diagram of the control assembly of the utility model;

[0028] Figure 5 It is a structural schematic diagram of another embodiment of the multi-channel dynamic pressurized explant in vitro culture device of the utility model.

[0029] Description of reference numerals:

[0030] 1. Control component; 11. PCB circuit board; 12. Microprocessor; 13. Power supply component; 14. Touch screen;

[0031] 2. Cover assembly; 21. Cover body; 22. Opening; 23. Electrical interface;

[0032] 3. Pressurizing assembly; 31. Pressing block; 32. Pressure sensor; 33. Electric telescopic rod;

[0033] 4. Cables; 5. Wireless transmission components; DETAILED DESCRIPTION

[0034] In order to further explain the technical means and effects adopted by the present invention to achieve the predetermined purpose of the utility model, the specific implementation method, structure, characteristics and effects of the multi-channel dynamic pressurized explant in vitro culture device proposed by the utility model are described in detail below in combination with the accompanying drawings and preferred embodiments.

[0035] like Figure 1-4 As shown, the multi-channel dynamic pressurized explant in vitro culture device of the utility model comprises:

[0036] Control component 1: In the pressurized explant in vitro culture device, the control component 1 is a very core part, which is used to realize the automation and intelligent control of the entire device.

[0037] The cover assembly 2 comprises a cover body 21, wherein the inner wall of the cover body 21 is provided with a plurality of pressurizing assemblies 3 corresponding to the respective holes of the orifice plate; each pressurizing assembly 3 is electrically connected to the control assembly 1; it should be noted that the specification and size of the cover body 21 can meet the requirements of the orifice plate to be covered; the cover body 21 is a cover body 21 for a 6-hole plate or a cover body 21 for a 24-hole plate;

[0038] Since it is necessary to realize multi-channel dynamic pressurization, the cover assembly 2 is provided to pressurize each channel in real time, so as to improve the working efficiency of the 3D mechanical pressurization culture of the explant and realize the purpose of adjustable and controllable pressure;

[0039] It should be further explained that the multi-channel dynamic pressurization explant in vitro culture device of the present application adopts a multi-channel dynamic pressurization design; since the sizes of the explants are different, the pressure sensor is used with high precision, and after contacting the explant, the signal is transmitted back to the microprocessor 12, at which time the pressure of 0.5N is preset to stop pressurization, ensuring that the pressure sensors in each channel and the explants are in full contact and will not slip. After waiting for all pressure sensors to contact the sample and are at the starting point of 0.5N, dynamic pressurization is started at the same time to reduce the differences between different batches, improve efficiency, and controllable test conditions and credible data.

[0040] Each group of pressurizing components 3 includes a press block 31, a pressure sensor 32 integrated at the bottom wall of the press block 31, and an electric telescopic rod 33 connected between the press block 31 and the cover body 21; each group of pressurizing components 3 plays a very critical role in the pressurized explant in vitro culture device, and it can provide stable and precise pressure stimulation for the explant.

[0041] In the present application, the pressing block 31 is the main part of the pressurizing component 3, and is usually made of a material that is resistant to high pressure, corrosion-resistant and has good biocompatibility, such as medical stainless steel; a pressure sensor 32 is integrated at the bottom wall of the pressing block 31 to monitor and measure the pressure of the pressing block 31 on the cells.

[0042] Furthermore, the electric telescopic rod 33 is connected between the pressure block 31 and the cover 21, and as a power transmission element, can adjust and maintain a stable pressure. When the pressure needs to be changed, the electric telescopic rod 33 can adjust its own length according to the control signal, thereby changing the pressure between the pressure block 31 and the cells; this design allows the pressurizing component 3 to achieve dynamic pressure adjustment to meet the different pressure requirements at different stages of the experiment.

[0043] Specifically, the control component 1 includes a PCB circuit board 11, a microprocessor 12 integrated on the PCB circuit board 11, and a power supply component 13; the PCB circuit board 11 is the core substrate of the entire control system, responsible for the connection of various electronic components and ensuring the stable transmission of current and signals; the microprocessor 12 is preferably a C51 single-chip microcomputer, which is responsible for receiving and parsing various input signals (such as instructions from the touch display screen 14, sensor data, etc.), and making decisions according to a predetermined program or algorithm, and then outputting corresponding control signals.

[0044] It also includes a touch display screen 14, which is electrically connected to the microprocessor 12; the touch display screen 14 is a human-computer interaction interface, allowing the user to intuitively view the current status of the device, set parameters, view historical data, etc.

[0045] By touching the display screen 14, the user can send instructions to the microprocessor 12 or modify the culture parameters so that the device can better meet the experimental needs.

[0046] The power supply assembly 13 is preferably a rechargeable battery, which provides a stable and continuous power supply for the entire control system. According to the needs of the device, the power supply assembly 13 may need to provide a variety of voltages and currents to meet the working requirements of different components.

[0047] It should be further explained that a power switch button and an adjustment knob are also provided on the outer wall of the control component 1, which are electrically connected to the microprocessor 12 respectively; they are used to control the opening and closing of the device and the adjustment of the pressure; of course, the pressure can also be controlled and adjusted on the touch display screen 14, and the real-time pressure value of each channel is displayed on the touch display screen 14.

[0048] See also Figure 1-4 The cover 21 is provided with a plurality of openings 22, and each opening 22 has an electrical interface 23 extending therethrough; the electrical interface 23 realizes data transmission with the control component 1 through the cable 4;

[0049] The opening 22 is sealed with a flexible rubber gasket.

[0050] Since the explant culture process needs to be sealed to prevent contamination by foreign matter, sealing pads are provided at the openings 22 to prevent foreign matter from entering from above.

[0051] Furthermore, it also includes a cable 4, one end of which is connected to the electrical interface 23 through each electrical plug, and the other end is plugged into the interface end on the control component 1.

[0052] Furthermore, the pressing block 31 is preferably made of medical stainless steel or polytetrafluoroethylene;

[0053] The pressure sensor 32 is preferably a thin film pressure sensor 32; the pressure sensor 32 is a key element for measuring the pressure of the pressure block 31 on the cells, and can monitor the pressure value in real time and accurately; the pressure sensor 32 integrated at the bottom wall of the pressure block 31 can more directly measure the surface pressure in contact with the explant, thereby ensuring that appropriate pressure stimulation is provided.

[0054] The multi-channel dynamic pressurized explant in vitro culture device of the present application is a 3D explant culture device for simulating the in vivo environment of an organism and providing dynamic pressure stimulation; it can provide dynamic pressure stimulation to simulate the pressure changes in the organism. By adjusting the parameters of the pressure stimulation, the effects of different pressures on cells in the explant can be studied; it can provide culture conditions that are closer to the in vivo environment of an organism, and provide more reliable experimental data and results for scientific research.

[0055] In some specific embodiments, see Figure 5 , further comprising a wireless transmission component 5, wherein the pressure sensor 32 and the microprocessor 12 use wireless transmission to perform data transmission;

[0056] Specifically, the wireless transmission component 5 may include a transmitter and a receiver, which are located at the pressure sensor and the microprocessor end respectively; the pressure sensor senses the pressure change in the culture environment and converts it into an electrical signal; this electrical signal is usually analog, and its voltage or current change is proportional to the pressure change; the electrical signal may need to pass through a conditioning circuit, such as amplification, filtering or linearization, to ensure the quality and accuracy of the signal; the conditioned signal is received by the wireless transmission component and sent to the receiving end where the microprocessor is located for analysis and processing through wireless communication technology (such as Bluetooth, Wi-Fi, Zigbee, etc.).

[0057] The present invention is further described above in conjunction with the embodiments, but the present invention is not limited to the above embodiments, and various changes can be made within the knowledge scope of ordinary technicians in this field without departing from the purpose of the present invention.

Claims

1. A multi-channel dynamic pressurized explant in vitro culture device, characterized in that: It includes: Control component (1); A cover assembly (2) comprises a cover body (21), wherein the inner wall of the cover body (21) is provided with a plurality of pressurizing assemblies (3) corresponding to each hole body at the orifice plate; each pressurizing assembly (3) is electrically connected to the control assembly (1) respectively; Each group of pressurizing components (3) comprises a press block (31), a pressure sensor (32) integrated at the bottom wall of the press block (31), and an electric telescopic rod (33) connected between the press block (31) and the cover body (21).

2. A multi-channel dynamic pressurized explant in vitro culture device according to claim 1, characterized in that: The control component (1) comprises a PCB circuit board (11), a microprocessor (12) integrated on the PCB circuit board (11), and a power supply component (13); It also includes a touch display screen (14), which is electrically connected to the microprocessor (12).

3. The multi-channel dynamic pressurized explant in vitro culture device according to claim 1, characterized in that: The cover body (21) is provided with a plurality of openings (22), and an electrical interface (23) is passed through each opening (22); The opening (22) is sealed with a flexible rubber gasket.

4. The multi-channel dynamic pressurized explant in vitro culture device according to claim 1, characterized in that: It also includes a cable (4), one end of which is connected to the electrical interface (23) through each electrical plug, and the other end is plugged into the interface end on the control component (1).

5. The multi-channel dynamic pressurized explant in vitro culture device according to claim 1, characterized in that: The pressing block (31) is preferably made of medical stainless steel or polytetrafluoroethylene; The pressure sensor (32) is preferably a thin-film pressure sensor (32).

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