Auxiliary tool for slowly adding NK cell culture blood

Abstract

The utility model discloses an auxiliary tool for slowly adding NK cell culture blood, which comprises a bottom plate, a test tube rack arranged at the top of the bottom plate and used for placing test tubes, and a support plate fixed at the top of the bottom plate, the transverse sliding rail is fixed to the side face of the supporting plate. The vertical sliding rail is in sliding connection with the transverse sliding rail; the auxiliary structure is in sliding connection with the vertical guide rail and can generate suction force and thrust force; the dropper is connected with the auxiliary structure and is used for temporarily storing the detected blood. According to the auxiliary tool for slowly adding the blood in the NK cell culture, the blood does not need to be manually controlled to be slowly dropped onto a separating medium, so that the convenience in use is increased, the operation burden is reduced, the speed can be better controlled, the operation stability is ensured, and the auxiliary tool is more convenient for a test.

Description

Technical Field

[0001] The utility model relates to the technical field of adding blood to NK cell culture, in particular to an auxiliary tool for slowly adding blood to NK cell culture. Background Art

[0002] NK cells (natural killer cells) are an important part of our immune system. They can recognize and kill virus-infected cells and certain types of cancer cells. In the laboratory, scientists often need to culture NK cells for various research.

[0003] In the process of NK cell culture, first of all, peripheral blood mononuclear cells (PBMCs) need to be collected and processed. Specifically, PBMCs are first isolated from the blood of healthy donors, and then PBMCs are mixed with nutrient-rich culture medium and growth factors are added.

[0004] In the process of isolating PBMCs from the blood of healthy donors, a certain amount of blood needs to be slowly added to a certain amount of separation fluid. This process needs to be carried out slowly to ensure that subsequent operations are not affected.

[0005] However, when currently in use, the blood drawing and slow dripping are all performed manually. The blood drawing operation is relatively convenient, but the slow dripping takes a long time. Not only is the user prone to hand soreness, but it is also difficult to control, which brings inconvenience to the user. Utility Model Content

[0006] In view of the deficiencies in the prior art, the utility model provides an auxiliary tool for slowly adding blood to NK cell culture, aiming to solve the above problems.

[0007] In order to achieve the above purpose, the utility model provides the following technical solutions:

[0008] An auxiliary tool for slowly adding blood to NK cell culture comprises a bottom plate, a test tube rack for placing test tubes is arranged on the top of the bottom plate, and further comprises:

[0009] a support plate fixed to the top of the base plate;

[0010] A transverse slide rail fixed to the side of the support plate;

[0011] A vertical slide rail, which is slidably connected to the horizontal slide rail;

[0012] An auxiliary structure, which is slidably connected to the vertical guide rail and can generate suction and thrust;

[0013] The dropper is connected to the auxiliary structure and is used for temporarily storing the blood to be tested.

[0014] Preferably, the vertical slide rail is fixed with a transverse slider, and the transverse slider is arranged inside the transverse slide rail for damping sliding.

[0015] Preferably, the auxiliary structure is fixed with a connecting block;

[0016] The connecting block is fixed with a vertical sliding block, and the vertical sliding block is arranged inside the vertical sliding rail for damping sliding.

[0017] Preferably, the auxiliary structure comprises:

[0018] A fixed shell, which is fixedly connected to the connecting block;

[0019] A piston chamber opened inside the fixed shell;

[0020] A piston slidably disposed inside the piston chamber;

[0021] An air hole is provided at the top of the fixed shell and communicates with the piston chamber;

[0022] A guide rod, which is fixed to the top of the piston and extends through the air hole to the top of the fixed shell;

[0023] a threaded post fixed to the top of the piston and extending through the stationary housing to the top of the stationary housing;

[0024] A driving structure, which is arranged at the fixed shell and is drivingly connected to the threaded column;

[0025] The switch is fixed to the side of the fixed shell and is electrically connected to the driving structure.

[0026] Preferably, a plug hole is provided at the bottom of the fixed shell, the test tube is plugged into the plug hole, and a sealing ring is provided.

[0027] Preferably, the driving structure comprises:

[0028] A rotating sleeve, the thread of which is sleeved on the outside of the threaded column;

[0029] The driven gear is fixedly sleeved on the outside of the rotating sleeve;

[0030] A motor fixedly connected to the fixed housing;

[0031] The driving teeth are fixed to the output shaft of the motor and mesh with the driven teeth.

[0032] Preferably, the number of teeth of the driving teeth is smaller than the number of teeth of the driven teeth.

[0033] Compared with the prior art, the utility model has the following beneficial effects:

[0034] The utility model provides an auxiliary tool for slowly adding blood to NK cell culture, which no longer needs manual control to slowly drip blood onto the separation liquid, thereby not only increasing the convenience during use and reducing the burden of operation, but also enabling better speed control, ensuring operational stability, and being more convenient for testing. BRIEF DESCRIPTION OF THE DRAWINGS

[0035] Figure 1 This is a schematic diagram of the structure of a blood slowly adding auxiliary tool for NK cell culture according to the utility model;

[0036] Figure 2 It is a side view of the auxiliary structure of the utility model;

[0037] Figure 3 It is an enlarged schematic diagram of the auxiliary structure of the utility model.

[0038] In the figure: 10 bottom plate, 11 test tube, 20 test tube rack, 30 support plate, 40 horizontal slide rail, 41 horizontal slider, 50 vertical slide rail, 51 connecting block, 52 vertical slider, 60 auxiliary structure, 61 fixed shell, 62 piston chamber, 63 piston, 64 air hole, 65 guide rod, 66 threaded column, 67 switch, 70 dropper, 80 driving structure, 81 rotating sleeve, 82 driven gear, 83 motor, 84 driving gear. DETAILED DESCRIPTION

[0039] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0040] An auxiliary tool for slowly adding blood to NK cell culture comprises a bottom plate 10, a test tube rack 20 for placing a test tube 11 is arranged on the top of the bottom plate 10, and the test tube 11 is a test tube commonly used for reaction;

[0041] In the examples of this application, please refer to Figure 1 and 2A support plate 30 is fixed on the top of the bottom plate 10, and a transverse slide rail 40 is fixed on the side of the support plate 30. A transverse slider 41 is provided for internal damping sliding of the transverse slide rail 40. The transverse slider 41 is fixedly connected to a vertical slide rail 50. The vertical slide rail 50 is slidably connected to the side of the transverse slide rail 40 through the transverse slider 41. The vertical slide rail 50 is internally damped slidingly provided with a transverse slide rail 40. A connecting block 51 is fixedly provided to the vertical slider 52. The connecting block 51 is fixedly connected to an auxiliary structure 60. The auxiliary structure 60 is connected to a dropper 70, which can generate suction and thrust for absorbing and discharging liquid. When in use, the left and right sliding auxiliary structure 60 and the vertical slide rail 50 can drive the dropper 70 to slide left and right to adapt to the positions of different test tubes 11. At the same time, the up and down sliding auxiliary structure 60 can drive the dropper 70 to enter the interior of the test tube 11, and then facilitate blood extraction and assembly to add blood.

[0042] In the above embodiment, the damping sliding connection can be achieved by existing methods, that is, it can stop at any time during the sliding process and will not move randomly. It can be completed by existing methods, such as increasing the friction of the sliding connection surface, or using damping slide rails, etc., depending on the actual situation. It only needs to be damped sliding. The innovation of this application does not lie in how to damp the sliding, so it will not be repeated.

[0043] For further examples, see Figure 1 , 2 3. The auxiliary structure 60 includes a fixed shell 61 fixedly connected to the connecting block 51. The bottom of the fixed shell 61 is provided with a plug hole, and the test tube 11 is plugged into the plug hole. A sealing ring is provided. When in use, the dropper 70 is disassembled and assembled as needed to facilitate replacement of the dropper 70.

[0044] A piston cavity 62 is provided inside the fixed shell 61, a piston 63 is slidably provided inside the piston cavity 62, an air hole 64 communicating with the piston cavity 62 is provided on the top of the fixed shell 61, a guide rod 65 and a threaded column 66 are fixed on the top of the piston 63, the guide rod 65 passes through the air hole 64 and extends to the top of the fixed shell 61, the threaded column 66 passes through the fixed shell 61 and extends to the top of the fixed shell 61, and a driving structure 80 drivingly connected to the threaded column 66 is provided at the fixed shell 61;

[0045] Specifically, the driving structure 80 includes a rotating sleeve 81 threadedly sleeved on the outside of the threaded column 66, a driven tooth 82 is fixedly sleeved on the outside of the rotating sleeve 81, a motor 83 is fixedly connected to the fixed shell 61, an output shaft of the motor 83 is fixedly provided with a driving tooth 84 meshing with the driven tooth 82, the number of teeth of the driving tooth 84 is less than the number of teeth of the driven tooth 82, a switch 67 electrically connected to the motor 83 is fixed on the side of the fixed shell 61, the motor 83 is an existing motor, preferably a stepping motor and a servo motor, and the switch 67 is a control switch of an existing motor, and the speed and rotation direction are controlled in an existing manner;

[0046] When in use, starting the motor 83 can drive the active gear 84 to rotate, thereby driving the driven gear 82 to rotate, and driving the rotating sleeve 81 to rotate, thereby driving the threaded column 66 to slide up and down, and finally driving the piston 63 to slide up and down. When the piston 63 slides downward, a thrust can be generated to push out the blood and liquid in the dropper 70, completing the slow pushing operation, and no longer requiring manual control by the user, thereby increasing stability and convenience during use;

[0047] On the contrary, the piston 63 moves upward to generate suction to absorb the liquid or the piston 63 .

[0048] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device. In the absence of further restrictions, the elements defined by the sentence "comprise a ..." do not exclude the existence of other identical elements in the process, method, article or device including the elements.

[0049] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. An auxiliary tool for slowly adding blood to NK cell culture, comprising a bottom plate, a test tube rack for placing test tubes is arranged on the top of the bottom plate, characterized in that: Also includes: a support plate fixed to the top of the base plate; A transverse slide rail fixed to the side of the support plate; A vertical slide rail, which is slidably connected to the horizontal slide rail; An auxiliary structure, which is slidably connected to the vertical guide rail and can generate suction and thrust; The dropper is connected to the auxiliary structure and is used for temporarily storing the blood to be tested.

2. The NK cell culture blood slow addition auxiliary tool according to claim 1, characterized in that: The vertical slide rail is fixed with a transverse sliding block, and the transverse sliding block is arranged inside the transverse slide rail for damping sliding.

3. The NK cell culture blood slow addition auxiliary tool according to claim 1, characterized in that: The auxiliary structure is fixed with a connecting block; The connecting block is fixed with a vertical sliding block, and the vertical sliding block is arranged inside the vertical sliding rail for damping sliding.

4. The NK cell culture blood slow addition auxiliary tool according to claim 1, characterized in that: The auxiliary structure comprises: A fixed shell, which is fixedly connected to the connecting block; A piston chamber opened inside the fixed shell; A piston slidably disposed inside the piston chamber; An air hole is provided at the top of the fixed shell and communicates with the piston chamber; a guide rod fixed to the top of the piston and extending through the air hole to the top of the stationary shell; a threaded post fixed to the top of the piston and extending through the stationary housing to the top of the stationary housing; A driving structure, which is arranged at the fixed shell and is drivingly connected to the threaded column; The switch is fixed to the side of the fixed shell and is electrically connected to the driving structure.

5. The NK cell culture blood slow addition auxiliary tool according to claim 4, characterized in that: The bottom of the fixed shell is provided with an inserting hole, the test tube is inserted into the inserting hole, and a sealing ring is arranged.

6. The NK cell culture blood slow addition auxiliary tool according to claim 4, characterized in that: The driving structure comprises: A rotating sleeve, the thread of which is sleeved on the outside of the threaded column; The driven gear is fixedly sleeved on the outside of the rotating sleeve; A motor fixedly connected to the fixed housing; The driving teeth are fixed to the output shaft of the motor and mesh with the driven teeth.

7. The NK cell culture blood slow addition auxiliary tool according to claim 6, characterized in that: The number of teeth of the driving teeth is smaller than the number of teeth of the driven teeth.

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