Multi-channel fluorescence and / or colorimetric detection device based on FPGA (Field Programmable Gate Array)

Abstract

The utility model discloses a multi-channel fluorescence and / or colorimetric detection device based on a field programmable gate array (FPGA). The scheme is provided aiming at the defects that the portability is poor and errors exist among a plurality of sensors in the prior art. A movement module is arranged in the center of the cavity of the device; a cuvette group is arranged around the movement module, and the cuvette group upwards penetrates through the upper end surface of the shell; setting the position of a first channel cuvette in the cuvette group as a preset first position, and driving the cuvettes to sequentially enter the preset first position through the movement module to perform fluorescence and / or colorimetric detection; a colorimetric sensor, a fluorescent sensor, a power supply, a voltage reduction module, a light source module, a switch, a display module, a fan and a control unit are arranged around the cavity. The system has the advantages that sampling, data processing and display are integrated; a colorimetric device and a fluorescence detection device are integrated; batch detection can be completed only by using two paths of sensors; the device is simple in structure, small in size, stable, high in integration and more convenient for batch detection.

Description

Technical Field

[0001] The utility model relates to a multi-channel fluorescence and / or colorimetric detection device based on FPGA. Background Art

[0002] In the prior art, for multi-channel fluorescence and / or colorimetric detection devices, sensors corresponding to the number of channels are generally used to detect fluorescence intensity and / or absorbance. For example, a multi-channel heavy metal detection device and detection method (CN113834789B) discloses the use of four photodiodes. There will be errors between the same sensors, and portable fluorescence detection devices on the market generally use mobile phones, computers, etc. to collect and process data. There are many hardware modules involved, and sampling, data processing, and display are often implemented by corresponding hardware modules, which cannot be truly portable. Summary of the invention

[0003] The utility model aims to provide a multi-channel fluorescence and / or colorimetric detection device based on FPGA to solve the problems existing in the above-mentioned prior art.

[0004] The utility model discloses a multi-channel fluorescence and / or colorimetric detection device based on FPGA, comprising a housing (115) provided with a cavity; a switch (112) and a display module (111) are provided on the front end surface of the housing (115), and a cooling fan (113) is provided on the rear end surface; a motion module (106) is provided at the center of the cavity; a cuvette group is provided around the motion module (106), and the cuvette group passes through the upper end surface of the housing upward; the position of the first channel cuvette (103) in the cuvette group is set as a preset first position, and the cuvettes are driven by the motion module (106) to enter the preset first position in sequence for fluorescence and / or colorimetric detection; and a colorimetric sensor (101), a fluorescence sensor (102), a power supply (108), a light source module (104), a step-down module (114) and a control unit (110) are provided around the cavity.

[0005] Optionally, the cuvette set includes a first channel cuvette (103), a second channel cuvette (105), a third channel cuvette (109) and a fourth channel cuvette (107).

[0006] Optional. The colorimetric sensor (101) and the fluorescence sensor (102) respectively send the collected light intensity values ​​in the colorimetric direction and the fluorescence direction to the control unit (110); the voltage of the power supply (108) is stepped down by the step-down module (114) to provide a working voltage for the control unit (110); the control unit (110) is used to control the motion module (106), the light source module (104) and the display module (111).

[0007] Optionally, the control unit (110) is specifically a FPGA-Altera-EP4CE10.

[0008] Optionally, the display module (111) is specifically a USART serial port screen.

[0009] Optionally, the motion module (106) comprises a rotation module and a stepping motor; the rotation module is provided with a groove for placing the cuvette set; and the stepping motor drives the rotation module to perform rotational motion.

[0010] The utility model discloses an FPGA-based multi-channel fluorescence and / or colorimetric detection device, which has the advantages of integrating sampling, data processing and display; batch detection can be completed by using only two sensors; the device has a simple structure, a small size, is stable and highly integrated, and is more convenient for batch detection. BRIEF DESCRIPTION OF THE DRAWINGS

[0011] Figure 1 It is a structural schematic diagram of the multi-channel fluorescence and / or colorimetric detection device described in the utility model.

[0012] Figure 2 It is a schematic diagram of the internal structure of the multi-channel fluorescence and / or colorimetric detection device described in the utility model.

[0013] Figure 3 It is a schematic diagram of the circuit principle of the multi-channel fluorescence and / or colorimetric detection device described in the utility model.

[0014] Figure 4 It is a comparison diagram of the fluorescence detection results of four channels in the multi-channel fluorescence and / or colorimetric detection device described in the utility model.

[0015] Figure 5 It is a comparison diagram of the colorimetric detection results of four channels in the multi-channel fluorescence and / or colorimetric detection device described in the utility model.

[0016] Reference numerals: colorimetric sensor (101), fluorescence sensor (102), first channel cuvette (103), light source module (104), second cuvette (105), motion module (106), fourth channel cuvette (107), power supply (108), third channel cuvette (109), control unit (110), display module (111), switch (112), fan (113), step-down module (114). DETAILED DESCRIPTION

[0017] FPGA (Field-Programmable Gate Array) is an integrated circuit chip that can be reconfigured by the user after manufacturing to achieve specific functions. Altera-EP4CE10 is an FPGA chip, one of the Cyclone IV series products produced by Altera (a subsidiary of Intel Corporation).

[0018] USART serial port screen usually refers to a display screen with a USART serial communication interface, which is used to communicate and exchange data with a control unit or other devices.

[0019] like Figures 1 to 3 As shown, a multi-channel fluorescence and / or colorimetric detection device for FPGA described in the utility model comprises a shell (115) provided with a cavity; a switch (112) and a display module (111) are provided on the front end surface of the shell (115), and a cooling fan (113) is provided on the rear end surface; a motion module (106) is arranged at the center of the cavity; a cuvette group is arranged around the motion module (106), and the cuvette group passes through the upper end surface of the shell upward; the position of the first channel cuvette (103) in the cuvette group is set as a preset first position, and the cuvettes are driven by the motion module (106) to enter the preset first position in sequence for fluorescence and / or colorimetric detection; and a colorimetric sensor (101), a fluorescence sensor (102), a light source module (104), a power supply (108), a step-down module (114) and a control unit (110) are arranged around the cavity. The cuvette group comprises a first channel cuvette (103), a second channel cuvette (105), a third channel cuvette (109) and a fourth channel cuvette (107). The colorimetric sensor (101) and the fluorescence sensor (102) respectively send the collected light intensity values ​​in the colorimetric direction and the fluorescence direction to the control unit (110); the voltage of the power supply (108) is stepped down by the step-down module (114) to provide a working voltage for the control unit (110); the control unit (110) is used to control the motion module (106), the light source module (104) and the display module (111).

[0020] The control unit (110) is specifically of FPGA-Altera-EP4CE10 model.

[0021] The display module (111) is specifically a USART serial port screen.

[0022] The motion module (106) comprises a rotation module and a stepping motor; the rotation module is provided with a groove for placing the cuvette set; the stepping motor drives the rotation module to perform rotational motion.

[0023] The working process of the FPGA-based multi-channel fluorescence and / or colorimetric detection device described in the present invention is specifically as follows, and is described by taking the fluorescence method for detecting casein phosphopeptides in dairy products as an example.

[0024] S101, turn on the switch (112); the voltage of the power supply (108) passes through the step-down module (114) to provide a suitable working voltage for the control unit (110), and the control unit (110) supplies power to the motion module (106), the display module (111), the light source module (104), the colorimetric sensor (101) and the fluorescence sensor (102).

[0025] S102, the control unit (110) uses pulse width modulation negative feedback to adjust and stabilize the light power of the light source module (104), with a wavelength of 420-425 nm.

[0026] S103, blank sample detection: insert a blank sample, touch the drop-down box on the display module (111) to select the sample type, and start the corresponding function to obtain the fluorescence intensity value F of the blank sample. 0 .

[0027] S104, test sample detection: put the test sample in, start the corresponding function to obtain the fluorescence intensity value F of the test sample.

[0028] Specifically, after placing the test sample, touch the Fluorescence button to obtain the fluorescence intensity value F of the test sample, and obtain the fluorescence intensity value F of the blank sample according to the linear equation and step S103. 0 , calculates and returns the content of casein phosphopeptide.

[0029] The specific linear equation is (taking dairy product 1 as an example):

[0030]

[0031] Where, C is the concentration of casein phosphopeptide, in μg / mL; F and F 0 are the fluorescence intensities of the test sample and the blank sample, respectively.

[0032] like Figure 4 As shown, Figure 4 This is a fluorescence detection comparison diagram of four channels in the multi-channel fluorescence and / or colorimetric detection device described in the utility model. Specifically, we prepared four identical test samples, each test sample was placed in four channels for three fluorescence intensity data readings, and error bars were made. When the device uses a single sensor for multi-channel reading, the relative standard deviation of the detection result is 1.33%. This shows that: the device can solve the problem of errors in the data read by multiple sensors in multiple channels in the prior art.

[0033] In addition, the working process of the FPGA-based multi-channel fluorescence and / or colorimetric detection device described in the present invention is specifically as follows, taking the colorimetric detection of lactose as an example.

[0034] S201, turn on the switch (112); the voltage of the power supply (108) passes through the step-down module (114) to supply power to the control unit (110), the motion module (106), the display module (111), the light source module (104), the colorimetric sensor (101) and the fluorescence sensor (102).

[0035] S202: The control unit (110) uses pulse width modulation negative feedback to adjust and stabilize the light emitting power of the light source module (104).

[0036] S203, blank sample detection: insert a blank sample, touch the drop-down box on the display module (111) to select the sample type, and start the corresponding function to obtain the absorbance A of the blank sample. 0 .

[0037] S204, test sample detection: put the test sample in, start the corresponding function to obtain the absorbance A of the test sample.

[0038] Specifically, after placing the test sample, touch the Absorbance button to obtain the absorbance A of the test sample, and obtain the absorbance A of the blank sample according to the linear equation and step S203. 0 , calculate A- A 0 And returns the test sample concentration.

[0039] The specific linear equation is (taking lactose as an example):

[0040]

[0041] Where, C is the concentration of lactose in mM; A and A 0 are the absorbance of the test sample and the blank sample, respectively.

[0042] like Figure 5 As shown, Figure 5 This is a colorimetric detection comparison diagram of four channels in the multi-channel fluorescence and / or colorimetric detection device described in the utility model. Specifically, we prepared four identical test samples, each test sample was placed in four channels for three absorbance data readings, and error bars were made. When the device uses a single sensor for multi-channel reading, the relative standard deviation of the test result is 0.24%. This shows that: the device can solve the problem of errors in the data read by multiple sensors in multiple channels in the prior art.

[0043] The volume size of the FPGA-based multi-channel fluorescence and / or colorimetric detection device described in the utility model is (100 mm*100 mm*100 mm), wherein the volume size of the rotating module is (50 mm*50 mm*50 mm).

[0044] The utility model discloses a multi-channel fluorescence and / or colorimetric detection device based on FPGA, which has the advantages of integrating sampling, data processing and display; integrating colorimetric and fluorescence detection devices; and completing batch detection by using only two sensors. The device has a simple structure, a small size, is stable and highly integrated, and is more convenient for batch detection.

[0045] For those skilled in the art, various other corresponding changes and deformations can be made according to the technical solutions and concepts described above, and all of these changes and deformations should fall within the protection scope of the claims of the utility model.

Claims

1. A multi-channel fluorescence and / or colorimetric detection device based on FPGA, characterized in that: include: A shell (115) is provided with a cavity; a switch (112) and a display module (111) are provided on the front end surface of the shell (115), and a cooling fan (113) is provided on the rear end surface; a motion module (106) is provided at the center of the cavity; a cuvette group is provided around the motion module (106), and the cuvette group passes through the upper end surface of the shell upwards; the position of the first channel cuvette (103) in the cuvette group is set as a preset first position, and the cuvettes are driven by the motion module (106) to enter the preset first position in sequence for fluorescence and / or colorimetric detection; and a colorimetric sensor (101), a fluorescence sensor (102), a power supply (108), a light source module (104), a step-down module (114), and a control unit (110) are provided around the cavity.

2. According to claim 1, a multi-channel fluorescence and / or colorimetric detection device based on FPGA, characterized in that: The cuvette set comprises a first channel cuvette (103), a second channel cuvette (105), a third channel cuvette (109) and a fourth channel cuvette (107).

3. According to claim 2, a multi-channel fluorescence and / or colorimetric detection device based on FPGA, characterized in that: The colorimetric sensor (101) and the fluorescence sensor (102) respectively send the collected light intensity values ​​in the colorimetric direction and the fluorescence direction to the control unit (110); the voltage of the power supply (108) is stepped down by the step-down module (114) to provide a working voltage for the control unit (110); the control unit (110) is used to control the motion module (106), the light source module (104) and the display module (111).

4. The FPGA-based multi-channel fluorescence and / or colorimetric detection device according to claim 1, characterized in that: The control unit (110) is specifically of FPGA-Altera-EP4CE10 model.

5. The FPGA-based multi-channel fluorescence and / or colorimetric detection device according to claim 1, characterized in that: The display module (111) is specifically a USART serial port screen.

6. The FPGA-based multi-channel fluorescence and / or colorimetric detection device according to claim 1, characterized in that: The motion module (106) comprises a rotation module and a stepping motor; the rotation module is provided with a groove for placing the cuvette set; the stepping motor drives the rotation module to perform rotational motion.

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