High-throughput cell sample integrated treatment equipment

Abstract

The utility model discloses high-throughput cell sample integrated treatment equipment, which comprises an equipment cabin, a treatment flow control device and an equipment function module, the treatment flow control device is in signal connection with the equipment function module, the treatment flow control device is arranged outside the equipment cabin, and the equipment function module is in signal connection with the equipment cabin. The equipment function module is fixedly mounted in the equipment cabin; the equipment function module comprises a centrifugal mechanism, a perforated plate transfer mechanism, a multi-channel pipetting mechanism, a constant-temperature culture module, a sample treatment platform, a glass slide suction mechanical arm, a supporting mechanism, a glass slide bin and a dispersion chamber. According to the application, cycle synchronization, suspension, separation, fixation and dispersion of cells are automatically completed, functional modules in culture, harvesting and slide preparation links in a sample treatment process are highly integrated, a perforated plate is used for replacing a traditional centrifugal tube for sample treatment, batch harvesting of samples and slide preparation treatment are realized, and the production efficiency is improved. The sample treatment flux and the sample treatment efficiency are improved, and meanwhile, the sample treatment consistency is also improved.

Description

Technical Field

[0001] The utility model relates to a biological sample processing instrument, in particular to a multi-well plate type high-throughput cell sample integrated processing device. Background Art

[0002] Cell sample processing equipment can automatically complete the process of harvesting metaphase chromosomes and cells from peripheral blood, bone marrow, amniotic fluid, umbilical cord blood, chorionic villus cells or other samples, and is used for analysis of chromosome karyotype, micronucleus and FISH. Harvesting cells for cytogenetic analysis is a multi-step process that can be done manually or by automated instruments. At present, such biological sample processing instruments have initially realized the automation of sample processing, but there are still many problems that need to be solved for the processing of large quantities of specimens.

[0003] Traditional automated sample processing equipment has low sample processing efficiency, slow sample processing flow, and long processing time from the first sample to the last sample, resulting in low consistency in sample processing. The quality of sample processing is affected by the throughput, making it difficult to achieve high-throughput sample processing. The processed samples are only filled in reagent tubes and cannot be dripped into glass slides for analysis in batches, resulting in low sample processing throughput and sample processing efficiency.

[0004] Chinese patent CN219495858 discloses a chromosome preparation device, including a sample loading cabinet, a culture cabinet, a harvest cabinet, a drip baking cabinet, a staining cabinet, and a conveying track; the sample loading cabinet is provided with a sample inlet, the staining cabinet is provided with a sample outlet, one end of the conveying track is arranged in the sample loading cabinet and located at the sample inlet, and the other end of the conveying track passes through the culture cabinet, the harvest cabinet, the drip baking cabinet, and the staining cabinet in sequence and passes through the sample outlet to the outside of the staining cabinet. The device simply connects the automation devices of the three links of culture, harvesting, and slicing in series to realize mechanical automated preparation. Each link uses a clamping mechanism and an operating table independently, the device occupies a large area, the structural setting is unreasonable, and it is difficult to achieve high-throughput preparation.

[0005] The multi-well plate cell sample pre-treatment equipment developed by Shanghai Lechen Biotechnology Co., Ltd. can only realize the harvesting function in the sample preparation pre-treatment process, cannot realize the integrated preparation, and cannot realize barcoding. Chinese patent CN220019093U (Shanghai Lechen Biotechnology Co., Ltd.) discloses a high-throughput automatic chromosome slice making machine, which can only realize the preparation function in the sample preparation pre-treatment process, and adopts the traditional centrifuge tube drop film, which has limited throughput and large floor space. Therefore, there is an urgent need for a highly integrated high-throughput cell sample integrated processing equipment to solve the above technical problems. Utility Model Content

[0006] In response to the problems existing in the prior art, the utility model provides a high-throughput integrated cell sample processing device, which highly integrates the functional modules of culture, harvesting and slice preparation in the sample processing process, replaces the traditional centrifuge tube with a multi-well plate for sample processing, and can drip into a glass slide in batches for analysis, greatly improving the sample processing throughput and sample processing efficiency, while also improving the consistency of sample processing.

[0007] In order to achieve the above purpose, the utility model adopts the following technical solutions:

[0008] A high-throughput cell sample integrated processing device, comprising an equipment cabin, a processing flow control device and an equipment function module, wherein the processing flow control device is connected to the equipment function module by signal, the processing flow control device is arranged outside the equipment cabin, and the equipment function module is fixedly installed inside the equipment cabin;

[0009] The functional modules of the equipment include a centrifugal mechanism, a multi-well plate transfer mechanism, a multi-channel pipetting mechanism, a constant temperature culture module, a sample processing platform, a glass slide suction mechanical arm, a support mechanism and a glass slide warehouse, and a dispersion chamber;

[0010] The sample processing platform is arranged at the bottom of the inner cavity of the equipment cabin; the support mechanism is fixedly installed inside the equipment cabin, and the support mechanism is arranged parallel to the sample processing platform;

[0011] The fixed parts of the multi-well plate transfer mechanism and the multi-channel pipetting mechanism are installed and fixed above the sample processing platform through the supporting mechanism; the sample processing platform is provided with a carrying area for placing or installing a pipetting tip, a sample processing reagent container, a waste liquid tank and a constant temperature culture module, a slide chamber, and a dispersion chamber;

[0012] The centrifugal mechanism and the dispersion chamber are arranged below the sample processing platform, and the sample processing platform exposes the top of the centrifugal mechanism and the dispersion chamber;

[0013] The multi-well plate is transferred within the range between the constant temperature culture module and the centrifugal mechanism by a multi-well plate transfer mechanism; the multi-well plate transfer mechanism is located above the sample processing platform; the multi-well plate transfer mechanism is provided with a gripping structure as a working component for taking and placing the multi-well plate;

[0014] The multi-channel pipetting mechanism is located above the sample processing platform; the multi-channel pipetting mechanism serves as a liquid adding mechanism to automatically add the processing reagent in the sample processing reagent container to the multi-well plate; the multi-channel pipetting mechanism also serves as a liquid suction mechanism to automatically remove the sample from the multi-well plate;

[0015] The constant temperature culture module is located on the table of the sample processing platform and serves as a temperature control device for performing constant temperature treatment on the samples in the multi-well plate;

[0016] The slide chamber is located on the table of the sample processing platform, and the slide chamber is arranged on one side of the dispersion chamber. The slide chamber serves as a placement component for a slide rack, and the slide rack fixes the slides;

[0017] The dispersion chamber is provided with a plurality of grooves for fixing the glass slide rack;

[0018] The slide suction mechanical arm is installed and fixed above the sample processing platform through the supporting mechanism; the slide suction mechanical arm is provided with a grasping structure as a working component for taking and placing the slide rack;

[0019] The processing flow control device is electrically connected to the centrifugal mechanism, the multi-well plate transfer mechanism, the multi-channel pipetting mechanism, the constant temperature culture module, and the slide suction mechanical arm.

[0020] Furthermore, the sample processing platform also includes an oscillation module; the oscillation module is installed on the sample processing platform; the oscillation module is provided with a perforated plate seat, a mounting frame, a drive motor and an eccentric block, the mounting frame is installed on the sample processing platform, the mounting frame is connected to the perforated plate seat, the drive motor is installed on the mounting frame, and the eccentric block is installed on the rotating shaft of the drive motor.

[0021] Furthermore, the dispersion chamber includes a barrel shell and a turntable, the turntable is arranged on the top of the barrel shell, a heating module, a humidification module and a refrigeration and dehumidification module are arranged in the barrel shell, the heating module, the humidification module and the refrigeration and dehumidification module are all located below the turntable, the heating module, the humidification module and the refrigeration and dehumidification module are all provided with a fan, and the upper surface of the turntable is provided with the groove.

[0022] Furthermore, the high-throughput cell sample integrated processing equipment also includes a waste gas adsorption treatment module; the waste gas adsorption treatment module is arranged on the top of the equipment cabin, and the waste gas adsorption treatment module serves as an absorption and transportation device for the gas in the equipment cabin; the waste gas adsorption treatment module includes a waste gas external exhaust fan, a waste gas adsorbent and a fan outer frame fixing frame; the waste gas external exhaust fan is installed on the top of the equipment cabin through the fan outer frame fixing frame; the waste gas adsorbent is arranged below the waste gas external exhaust fan.

[0023] Furthermore, the centrifugal mechanism includes a centrifugal chamber, a driving mechanism and a sample hanging basket, the centrifugal chamber is a closed structure, the centrifugal process of the sample is carried out in the centrifugal chamber, and the sample hanging basket is used to place a porous plate containing samples to be processed; the driving mechanism includes a centrifugal motor, a motor driver and a transmission mechanism; the centrifugal motor is located at the lower part of the centrifugal mechanism, and the power output end of the centrifugal motor is connected to the sample hanging basket through the transmission mechanism; the centrifugal motor is driven and controlled by the motor driver, and the processing flow control device controls the centrifugal motor through the motor driver to perform centrifugal operations on the samples in the porous plate on the sample hanging basket.

[0024] Furthermore, the porous plate transfer mechanism includes a first three-dimensional motion mechanism and a porous plate clamping mechanism; the first three-dimensional motion mechanism is used to drive the porous plate clamping mechanism to perform three-dimensional movement of the porous plate, and the porous plate clamping mechanism is used to grab the porous plate so that the grabbed porous plate can be operated between the centrifugal mechanism, the constant temperature culture module and the sample processing platform.

[0025] Furthermore, the multi-channel pipetting mechanism includes a second three-dimensional motion mechanism and a multi-channel pipetting module; the second three-dimensional motion mechanism drives the multi-channel pipetting module to move, and the multi-channel pipetting module is used to add reagents to or remove reagents from the multi-well plate; the number of pipetting channels of the multi-channel pipetting module is consistent with the number of holes of the multi-well plate; the multi-channel pipetting module is a 1 to 96-channel pipetting module.

[0026] Furthermore, the slide suction robot includes a third three-dimensional motion mechanism and a slide rack clamping mechanism; the third three-dimensional motion mechanism is used to drive the slide rack clamping mechanism to perform three-dimensional motion on the slide rack, and the slide rack clamping mechanism is used to grab the slide rack so that the grabbed slide rack can move between the constant temperature culture module, the slide bin, and the dispersion chamber.

[0027] Furthermore, the constant temperature culture module includes a temperature control module, a porous plate placement cavity and a moving base bracket; the temperature control module is composed of a temperature sensor and a temperature control element, and is used to control the temperature inside the porous plate placement cavity; the porous plate placement cavity is a closed cavity with an insulating material on the outside, and is used to place the porous plate containing the processed samples; the moving base bracket is composed of a porous plate base, a traction motor and a motion mechanism, and is used to operate the porous plate containing the processed samples inside and outside the porous plate placement cavity, and to enable the moving base bracket to perform horizontal pulling and transmission inside and outside the porous plate placement cavity, so as to realize constant temperature treatment of the samples and transportation operations of the porous plates.

[0028] Furthermore, on the sample processing platform, the constant temperature culture module is arranged near the edge of one side of the sample processing platform, the pipette tip is arranged near the edge of the other side of the sample processing platform, the waste liquid tank, the pipette tip, and the dispersion chamber are located in the middle area of ​​the sample processing platform, and the sample processing reagent container is arranged near the placement position of the pipette tip.

[0029] The above modules constitute a complete biological sample processing instrument. Under the control of the processing flow control device, the automated processing of biological samples is completed, and the automation of the following processing flows is realized:

[0030] 1. Automatically centrifuge the samples in the multi-well plate to separate the samples;

[0031] 2. Automatically take, place and transfer the multi-well plate, and realize the operation of the multi-well plate between the centrifugal mechanism, platform and constant temperature culture module;

[0032] 3. Automatically remove the supernatant of samples in the multi-well plate;

[0033] 4. Automatically add sample treatment reagents to the sample wells of the multi-well plate, including synchronization treatment reagents, autumn water solution, hypotonic solution and fixative solution;

[0034] 5. Automatically mix samples;

[0035] 6. Automatically control the temperature of the sample processing process;

[0036] 7. The processed samples can be dropped into the slide in batches for analysis, and the processing flow data information can be automatically generated.

[0037] Beneficial effects of the utility model:

[0038] 1. This device replaces the traditional centrifuge tube with a multi-well plate, and achieves a higher throughput of sample processing through the sample processing mode of the multi-well plate;

[0039] 2. This device uses a multi-channel pipetting module to achieve a more efficient sample processing process, reduce the processing differences between different samples, further improve the consistency of sample processing, and achieve a higher degree of standardization of sample processing;

[0040] 3. This device can realize batch harvesting of samples and slide preparation by setting the harvesting and slide dropping parameters, thereby improving the sample processing throughput and sample processing efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0041] Figure 1 This is a schematic diagram of the overall structure of the high-throughput cell sample integrated processing device of the utility model;

[0042] Figure 2This is a schematic diagram of the internal structure of the high-throughput cell sample integrated processing device of the utility model;

[0043] Figure 3 It is a structural schematic diagram of the sample processing platform of the utility model;

[0044] Figure 4 This is a schematic diagram of the structure of the oscillation module of the utility model;

[0045] Figure 5 This is a schematic diagram of the structure of the dispersion chamber of the utility model;

[0046] Figure 6 This is a schematic diagram of the internal structure of the dispersion chamber of the utility model;

[0047] Figure 7 This is a schematic diagram of the structure of the waste gas adsorption treatment module of the utility model;

[0048] Figure 8 This is a schematic diagram of the structure of the utility model in which the multi-well plate is arranged in a constant temperature culture module;

[0049] Fig. 9 This is a schematic diagram of the centrifugal mechanism structure of the utility model;

[0050] Fig.10 This is a schematic diagram of the structure of the porous plate transfer mechanism of the utility model;

[0051] Fig.11 This is a schematic diagram of the structure of the multi-channel liquid transfer mechanism of the utility model;

[0052] Fig.12 This is a schematic diagram of the structure of the glass slide suction mechanical arm of the utility model;

[0053] Fig.13 This is a schematic diagram of the structure of the constant temperature culture module of the utility model.

[0054] The markings in the figure are as follows:

[0055] Equipment cabin 1, processing flow control device 2, equipment function module 3, waste gas adsorption treatment module 4, centrifugal mechanism 31, centrifugal cavity 311, centrifugal motor 312, transmission mechanism 313, sample hanging basket 314, multi-well plate transfer mechanism 32, first three-dimensional motion mechanism 321, multi-well plate clamping mechanism 322, multi-channel pipetting mechanism 33, second three-dimensional motion mechanism 331, multi-channel pipetting module 332, constant temperature culture module 34, temperature control module 341, multi-well plate placement cavity 342, motion base bracket 343, traction motor 344, sample processing platform 35, sample processing reagent container Device 351, waste liquid tank 352, pipette tip 353, oscillation module 354, well plate seat 3541, mounting frame 3542, drive motor 3543, eccentric block 3544, slide suction robot arm 36, third three-dimensional motion mechanism 361, slide rack clamping mechanism 362, support mechanism 37, slide bin 38, slide rack 381, dispersion chamber 39, cylinder shell 391, turntable 392, heating module 393, humidification module 394, refrigeration and dehumidification module 395, exhaust gas exhaust fan 41, exhaust gas adsorbent 42, fan outer frame fixing frame 43, porous plate 5, sample hole 51, scanning module 6. DETAILED DESCRIPTION

[0056] The utility model is further described below in conjunction with the accompanying drawings.

[0057] Example 1

[0058] In this embodiment, see Figure 1 , Figure 2 A high-throughput cell sample integrated processing device includes an equipment cabin 1, a processing flow control device 2 and an equipment function module 3. The processing flow control device 2 is signal-connected to the equipment function module 3. The processing flow control device 2 is arranged outside the equipment cabin 1, and the equipment function module 3 is fixedly installed inside the equipment cabin 1; the processing flow control device 2 includes a personal computer or a touch screen.

[0059] See also Figure 2 , Figure 3 The equipment functional module 3 includes a centrifugal mechanism 31, a multi-well plate transfer mechanism 32, a multi-channel pipetting mechanism 33, a constant temperature culture module 34, a sample processing platform 35, a glass slide suction robot 36, a support mechanism 37, a glass slide warehouse 38 and a dispersion chamber 39.

[0060] The sample processing platform 35 is arranged at the bottom of the inner cavity of the equipment cabin 1; the support mechanism 37 is fixedly installed inside the equipment cabin 1, and the support mechanism 37 is arranged parallel to the sample processing platform 35; the fixed parts of the multi-well plate transfer mechanism 32 and the multi-channel pipetting mechanism 33 are installed and fixed on the sample processing platform 35 through the support mechanism 37; the sample processing platform 35 is provided with a carrying area for placing or installing a pipette tip 353, a sample processing reagent container 351, a waste liquid tank 352 and a constant temperature culture module 34, a slide warehouse 38, and a dispersion chamber 39.

[0061] The centrifugal mechanism 31 and the dispersion chamber 39 are arranged below the sample processing platform 35. The sample processing platform 35 is provided with a first through hole for fixing the centrifugal mechanism 35 and a second through hole for fixing the dispersion chamber 39. The centrifugal mechanism 35 is fixed in the first through hole, and the dispersion chamber 39 is fixed in the second through hole, so that the sample processing platform 35 exposes the top of the centrifugal mechanism 31 and the dispersion chamber 39 through the first through hole and the second through hole respectively; that is, an opening (the first through hole and the second through hole) is respectively opened at the same position at the bottom of the equipment cabin 1 and on the sample processing platform 35 above the centrifugal mechanism 31 and the dispersion chamber 39, through which the centrifugal mechanism 31 and the dispersion chamber 39 are installed at the opening; the porous plate 5 is transferred within the range between the constant temperature culture module 34 and the centrifugal mechanism 31 through the porous plate transfer mechanism 32.

[0062] The multi-porous plate 5 is transported within the range between the constant temperature culture module 34 and the centrifugal mechanism 31 through the multi-porous plate transfer mechanism 32; the multi-porous plate transfer mechanism 32 is located above the sample processing platform 35; the multi-porous plate transfer mechanism 32 is provided with a gripping structure as a working component for taking and placing the multi-porous plate 5.

[0063] The multi-channel pipetting mechanism 33 is located above the sample processing platform 35; the multi-channel pipetting mechanism 33 serves as a liquid adding mechanism to automatically add the processing reagent in the sample processing reagent container 351 to the multi-well plate 5; the multi-channel pipetting mechanism 33 also serves as a liquid suction mechanism to automatically remove the sample from the multi-well plate 5; the multi-channel pipetting mechanism 33 is arranged in the equipment cabin 1 as an integrated liquid adding and liquid removing automated equipment.

[0064] The single-channel pipetting mechanism is located above the sample processing platform 35 ; as a pipetting mechanism, the single-channel pipetting mechanism automatically transfers the sample in the multi-well plate 5 to the surface of the slide to realize the sample preparation operation.

[0065] The constant temperature culture module 34 is located on the table of the sample processing platform 35 and serves as a temperature control device for performing constant temperature treatment on the samples in the multi-well plate 5 .

[0066] The slide chamber 38 is located on the table of the sample processing platform 35 . The slide chamber 38 is arranged on one side of the dispersion chamber 39 . The slide chamber 38 serves as a placement component for the slide rack 381 . The slide rack 381 fixes the slide.

[0067] The dispersion chamber 39 is provided with a plurality of grooves for fixing the glass slide rack 381 .

[0068] The slide suction robot 36 is installed and fixed above the sample processing platform 35 through a supporting mechanism 37 ; the slide suction robot 36 is provided with a grasping structure as a working component for taking and placing the slide rack 381 .

[0069] The processing flow control device 2 is electrically connected to the centrifugal mechanism 31 , the multi-well plate transfer mechanism 32 , the multi-channel pipetting mechanism 33 , the constant temperature culture module 34 , and the slide suction robot 36 .

[0070] See also Figure 3 , Figure 4 The sample processing platform 35 also includes an oscillation module 354; the oscillation module 354 is installed on the sample processing platform 35; the oscillation module 354 is provided with a perforated plate seat 3541, a mounting frame 3542, a driving motor 3543 and an eccentric block 3544, the mounting frame 3542 is installed on the sample processing platform 35, the mounting frame 3542 is connected to the perforated plate seat 3541, the driving motor 3543 is installed on the mounting frame 3542, and the eccentric block 3544 is installed on the rotating shaft of the driving motor 3543.

[0071] See also Figure 5 , Figure 6 The dispersion chamber 39 includes a cylinder shell 391 and a turntable 392. The turntable 392 is arranged on the top of the cylinder shell 391. A heating module 393, a humidifying module 394 and a refrigeration and dehumidification module 395 are arranged in the cylinder shell 391. The heating module 393, the humidifying module 394 and the refrigeration and dehumidification module 395 are all located below the turntable 392. The heating module 393, the humidifying module 394 and the refrigeration and dehumidification module 395 are all provided with fans, and a groove is arranged on the upper surface of the turntable 392.

[0072] See also Figure 2 , Figure 7 The high-throughput cell sample integrated processing equipment also includes a waste gas adsorption processing module 4; the waste gas adsorption processing module 4 is arranged on the top of the equipment cabin 1, and the waste gas adsorption processing module 4 serves as an absorption and transportation device for the gas in the equipment cabin 1.

[0073] The high-throughput cell sample integrated processing equipment of this embodiment includes a centrifugal mechanism 31, a multi-well plate transfer mechanism 32, a multi-channel pipetting mechanism 33, a constant temperature culture module 34, a sample processing platform 35 and an exhaust gas adsorption processing module 4. The multi-channel pipetting mechanism 33 can automatically add the processing reagent 51 to the multi-well plate 5 to realize the processing of the sample by different processing reagents; at the same time, it has the function of automatically removing the supernatant from the sample wells in the multi-well plate 5 to realize the automation of adding and removing liquid. As a device for replacing traditional centrifuge tubes with multi-well plates for sample processing, the equipment of this embodiment is applied to the sample preparation process in the process of cell genetic analysis, which greatly improves the sample processing throughput and sample processing efficiency, and also improves the consistency of sample processing.

[0074] The slide chamber 38 is arranged on one side of the dispersion chamber 39. The slide chamber 38 serves as a placement component for the slide rack 381, and the slide rack 381 fixes the slide. The dispersion chamber 39 is provided with a plurality of grooves for fixing the slide rack 381. By arranging the slide and the dispersion chamber 39, the processed samples can be automatically dripped into the slide in batches for analysis, thereby realizing integrated batch analysis processing of samples and improving the sample processing throughput and sample processing efficiency.

[0075] In this embodiment, see Figure 8 The multi-well plate 5 is a multi-well plate structure. The inner cavity volume of each sample hole 51 is 1 to 2.2 mL, which is used to place samples and culture cell samples; during the sample processing, different sample processing reagents are added to the sample hole 51 to realize the automation of the sample processing process. Commonly used multi-well plates include 4, 8, 12, 24, 48 or 96-hole multi-well plates, preferably 96-hole multi-well plates, to achieve high-throughput sample processing. The inner cavity volume of the sample hole 51 is 1 to 2.2 mL. At least 2 multi-well plates are processed at a time, and the sample processing throughput is 192 at a time, which is twice the traditional maximum cell sample processing equipment. 4 multi-well plates are processed at a time, and the sample processing throughput is 384 at a time, which is 4 times the traditional maximum cell sample processing equipment, realizing high-throughput sample processing. By cooperating with the high-throughput multi-channel pipetting mechanism 33, batch processing of samples is realized. The utility model adopts a multi-well plate to replace the traditional centrifuge tube for sample processing equipment, which is applied to the sample preparation process in the process of cell genetic analysis, and greatly improves the sample processing throughput and sample processing efficiency.

[0076] Preferably, the utility model replaces the traditional centrifuge tube with the porous plate 5, thereby realizing that one porous plate can process multiple samples, and the processing capacity is significantly improved compared with the centrifuge tube. The utility model preferably uses the porous plate 5 to process samples, preferably 2 or 4 porous plates 5 are processed per batch, each porous plate 5 can process 96 samples, and a total of up to 196 or 384 samples can be processed simultaneously per batch. Compared with traditional such automated equipment, the sample processing throughput is many times that of traditional sample processing equipment. If the number of porous plates processed per batch is increased, higher throughput sample processing can be achieved, which has significant advantages compared with traditional automated sample processing equipment.

[0077] Example 2

[0078] This embodiment is basically the same as the above embodiment, with the following features:

[0079] In this embodiment, see Fig. 9 , schematic diagram of the centrifugal mechanism structure used.

[0080] The centrifugal mechanism 31 of the high-throughput cell sample integrated processing device of this embodiment is composed of a centrifugal chamber 311, a driving mechanism and a sample hanging basket 314. The centrifugal chamber 311 is a closed structure. The centrifugation process of the sample is carried out in the centrifugal chamber 311. The sample hanging basket 314 is used to place the porous plate 5 containing the samples to be processed.

[0081] In this embodiment, the preferred driving mechanism includes a centrifugal motor 312, a motor driver and a transmission mechanism 313; the centrifugal motor 312 is located at the lower part of the centrifugal mechanism 31, and the power output end of the centrifugal motor 312 is connected to the sample hanging basket 314 through the transmission mechanism 313; the centrifugal motor 312 is driven and controlled by the motor driver, and the processing flow control device 2 controls the centrifugal motor 312 through the motor driver to perform centrifugal operation on the sample in the porous plate 5 on the sample hanging basket 314.

[0082] The centrifugal chamber 311 of the present embodiment is a closed structure, and the centrifugal process of the sample is carried out in the centrifugal chamber, which ensures the safety of the centrifugal process and reduces the noise generated in the centrifugal process. This design is similar to a conventional centrifuge. The sample hanging basket 314 is used to place the porous plate 5 with the sample to be processed, and the placement operation process is automatically completed by the porous plate transfer mechanism 32. To ensure the balance of the centrifuge, the sample hanging basket 314 is a symmetrical design, and the number is set to an even number such as 2,4,6,8, etc., which is at least 2. The centrifugal motor 312 is located below the centrifugal mechanism 31, is connected with the sample hanging basket 314 by a transmission mechanism 313, and realizes the centrifugal treatment of the porous plate sample. The centrifugal motor 312 is driven and controlled by a motor driver, and the processing flow control device 2 controls the motor to rotate at a set speed by the motor driver, and the sample in the porous plate 5 on the sample hanging basket is centrifuged.

[0083] Example 3

[0084] This embodiment is basically the same as the above embodiment, with the following features:

[0085] In this embodiment, see Fig.10 , schematic diagram of the structure of the porous plate transfer mechanism used.

[0086] The porous plate transfer mechanism 32 is composed of a first three-dimensional motion mechanism 321 and a porous plate clamping mechanism 322; the first three-dimensional motion mechanism 321 is used to drive the porous plate clamping mechanism 322 to perform three-dimensional motion on the porous plate 5, and the porous plate clamping mechanism 322 is used to grab the porous plate 5 so that the grabbed porous plate 5 can be operated between the centrifugal mechanism 31, the constant temperature culture module 34 and the sample processing platform 35.

[0087] The first three-dimensional motion mechanism 321 of this embodiment is used to drive the porous plate clamping mechanism 322 to perform three-dimensional motion on the porous plate 5, so as to realize the automatic transportation of the porous plate 5 between the constant temperature culture module 34 and the centrifugal mechanism 31. Preferably, the porous plate clamping mechanism 322 is composed of an electric clamp and an electric finger. By opening and closing the electric clamp, the electric finger realizes the pick-up and placement operation of the porous plate 5. The first three-dimensional motion mechanism 321 is composed of an X-axis motor, a Y-axis motor, a Z-axis motor and a corresponding transmission mechanism, and the transportation operation of the porous plate 5 is realized through the combined movement of the three motors. The transmission mechanism is composed of a guide rail, a screw rod or a synchronous belt, which converts the rotational motion of the motor into a linear motion.

[0088] Example 4

[0089] This embodiment is basically the same as the above embodiment, with the following features:

[0090] In this embodiment, see Fig.11 , schematic diagram of the structure of the multi-channel pipetting mechanism used.

[0091] The multi-channel pipetting mechanism 33 is composed of a second three-dimensional motion mechanism 331 and a multi-channel pipetting module 332 ; the second three-dimensional motion mechanism 331 drives the multi-channel pipetting module 332 to move, and the multi-channel pipetting module 332 is used to add reagents to the multi-well plate 5 or remove reagents.

[0092] The number of pipetting channels of the multichannel pipetting module 332 is preferably consistent with the number of wells of the multi-well plate; the multichannel pipetting module 332 is a 1-96 channel pipetting module. The multichannel pipetting module 332 is used to add reagents to and remove reagents from the multi-well plate 5, and to mix the samples in the multi-well plate 5.

[0093] The multi-channel pipetting module 332 of this embodiment is a multi-channel pipetting module. The number of pipetting channels of the multi-channel pipetting module 332 is consistent with the number of sample holes 51 of the porous plate 5. A 96-channel pipetting module is preferably used to achieve high efficiency in sample processing. The main functions of the multi-channel pipetting module 332 include the taking and placing of liquids and the mixing operation of samples. The single liquid absorption, in conjunction with the movement of the three-dimensional motion mechanism, transfers the waste liquid from the sample hole 51 to the waste liquid tank 352; the waste liquid removal operation is realized, and the sample treatment reagent 51 is added to the sample hole 51 to realize the processing operation of different reagents on the sample. Multiple liquid suction and discharge operations are performed in the sample hole 51 to achieve the mixing operation of the sample. The three-dimensional motion mechanism is used to drive the pipetting module to perform three-dimensional movement, realize the transfer operation of waste liquid and the addition operation of sample treatment reagent. The three-dimensional motion mechanism is composed of an X-axis motor 32, a Y-axis motor 34, a Z-axis motor 33 and a corresponding transmission mechanism 35. The transport operation of the porous plate 5 is realized through the combined movement of the three motors. The transmission mechanism 35 is composed of a guide rail, a lead screw and a synchronous belt, and converts the rotational motion of the motor into linear motion.

[0094] Preferably, the number of channels of the multi-channel pipetting module 332 is preferably 8 or 96, so as to achieve high throughput and high efficiency of sample processing. The 8-channel pipetting module can perform liquid addition, liquid aspiration and mixing operations on 8 samples at the same time, and the 96-channel pipetting module can perform liquid addition, liquid aspiration and mixing operations on 96 samples at the same time, so as to achieve higher throughput sample processing efficiency. The multi-channel pipetting module 332 is fixedly mounted on the three-dimensional motion mechanism, and the movement of the pipetting module between different reagent tanks and samples is realized through three-dimensional motion. The multi-channel pipetting module 332 aspirates liquid from the reagent tank and adds it to the sample well of the porous plate 5 to process the sample; the multi-channel pipetting module 332 aspirates liquid from the porous plate 5 after centrifugation, and can remove the supernatant to realize the separation operation of the sample; the multi-channel pipetting module 332 realizes the mixing operation of the sample in the porous plate 5 by aspirating and discharging the sample in the porous plate 5. The liquid addition, liquid aspiration and mixing operations are controlled by a program to realize the full automation of the whole process of sample processing.

[0095] Preferably, a multi-channel multi-channel pipetting module 332 is used to replace the traditional sample adding needle for adding and aspirating liquid. Existing commercial automated sample processing equipment of this type uses a maximum of 8-channel liquid adding needles and liquid aspiration needles, which is inefficient. The multi-channel pipetting module 332 used in this utility model can have up to 96 channels, which is more efficient.

[0096] Example 5

[0097] This embodiment is basically the same as the above embodiment, with the following features:

[0098] In this embodiment, see Fig.12 , a schematic diagram of the structure of the glass slide suction robot 36 used.

[0099] The slide suction robot 36 includes a third three-dimensional motion mechanism 361 and a slide rack clamping mechanism 362; the first three-dimensional motion mechanism 321 is used to drive the slide rack clamping mechanism 362 to perform three-dimensional motion on the slide rack 381, and the slide rack clamping mechanism 362 is used to grab the slide rack 381, so that the grabbed slide rack 381 moves between the constant temperature culture module 34, the slide bin 38, and the dispersion chamber 39. The slide rack clamping mechanism 362 is preferably an electric clamp.

[0100] Preferably, the slide rack clamping mechanism 362 is composed of an electric gripper and an electric finger. The electric finger realizes the pick-up and placement operation of the slide rack 381 by opening and closing the electric gripper. The third three-dimensional motion mechanism 361 is composed of an X-axis motor, a Y-axis motor, a Z-axis motor and a corresponding transmission mechanism. The combined movement of the three motors realizes the transport operation of the slide rack 381. The transmission mechanism is composed of a guide rail, a screw rod or a synchronous belt, which converts the rotational motion of the motor into a linear motion.

[0101] Example 6

[0102] This embodiment is basically the same as the above embodiment, with the following features:

[0103] In this embodiment, see Fig.13 , a schematic diagram of the structure of the constant temperature culture module 34 used.

[0104] The constant temperature culture module 34 is composed of a temperature control module 341, a porous plate placement cavity 342 and a moving base bracket 343; the temperature control module 341 is composed of a temperature sensor and a temperature control element, and is used to control the temperature inside the porous plate placement cavity 342. The porous plate placement cavity 342 is a closed cavity with an insulating material on the outside, and is used to place the porous plate 5 containing the processed samples. The moving base bracket 343 is composed of a porous plate base, a traction motor 344 and a motion mechanism, and is used to operate the porous plate 5 containing the processed samples inside and outside the porous plate placement cavity 342, and to enable the moving base bracket 343 to perform horizontal pulling and transmission inside and outside the porous plate placement cavity 342, so as to realize the constant temperature treatment of the samples and the transportation operation of the porous plate 5.

[0105] Preferably, the moving base bracket 343, driven by the motor, can transfer the multi-porous plate 5 from the cavity of the constant temperature culture module to the cavity outside the constant temperature culture module 34, which is convenient for the multi-channel pipetting module 332 to add liquid, aspirate and mix samples into the multi-porous plate 5, and at the same time, it is also convenient for the multi-porous plate clamping mechanism 322 to take and transfer the multi-porous plate. The temperature control module 341 is used to adjust the temperature inside the cavity of the constant temperature culture module 34 to achieve constant temperature treatment during the sample processing process, avoid the sample processing process being affected by changes in ambient temperature, and is conducive to achieving standardization of the sample processing process. The temperature sensor is a temperature sensor such as a Pt100 thermistor and a K-type temperature sensor.

[0106] The temperature control module 341 of this embodiment detects the temperature inside the porous placement cavity 42 through a temperature sensor, and then controls the temperature inside the cavity 42 within a set range to achieve constant temperature treatment of the sample in the porous plate 5. The porous plate 5 containing the sample is placed on the moving base bracket 343, and driven by the motor 44, the moving base bracket 343 is pulled and transported horizontally inside and outside the porous plate placement cavity 342 to achieve constant temperature treatment of the sample and transportation of the porous plate.

[0107] Example 7

[0108] In this embodiment, see Figure 7 , schematic diagram of the structure of the waste gas adsorption treatment module used.

[0109] The exhaust gas adsorption treatment module 4 is arranged on the top of the equipment cabin 1, and the exhaust gas adsorption treatment module 4 serves as an absorption and transportation device for the gas in the equipment cabin 1; the exhaust gas adsorption treatment module 4 includes an exhaust gas external exhaust fan 41, an exhaust gas adsorbent 42 and a fan outer frame fixing frame 43; the exhaust gas external exhaust fan 41 is installed on the top of the equipment cabin 1 through the fan outer frame fixing frame 43; the exhaust gas adsorbent 42 is arranged below the exhaust gas external exhaust fan 41.

[0110] The exhaust gas adsorption treatment module 4 is used to adsorb and treat harmful gases generated during the experiment. The exhaust gas adsorbent 42 is preferably an activated carbon adsorbent, activated carbon and potassium permanganate mixed particles. The exhaust gas exhaust fan is a turbofan fan or a DC fan. Driven by the fan 61, the air containing harmful exhaust gas inside the equipment is absorbed and treated by the exhaust gas adsorbent 42, and then discharged into the environment, which plays a protective role on the environment and the operators of the equipment.

[0111] Example 8

[0112] In this embodiment, see Figure 2 , Figure 3 , schematic diagram of the sample processing platform structure used.

[0113] On the sample processing platform 35, the constant temperature culture module 34 is arranged near the edge of one side of the sample processing platform 35, the pipette tip 53 is arranged near the edge of the other side of the sample processing platform 35, the waste liquid tank 352 and the pipette tip 53 are located in the middle area of ​​the sample processing platform 35, and the sample processing reagent container 351 is arranged near the placement position of the pipette tip 53.

[0114] Preferably, another area of ​​the sample processing platform 35 is used as an area for placing the centrifuge chamber cover 55 .

[0115] Preferably, the sample processing platform 35 is mainly composed of a table 56 and multiple modules installed on the table 56, which are used to place the pipette tip 53, the sample processing reagent 51, the constant temperature culture module 34, the waste liquid tank 352 and the centrifugal chamber cover 55. The surface of the table 56 is provided with an opening 54, through which the multi-well plate transfer mechanism 32 realizes the transfer operation of the multi-well plate 5 between the constant temperature culture module 34 and the centrifugal mechanism 31.

[0116] Preferably, the utility model can reduce the traditional 5mL culture medium system to a 1-2ml culture system, which controls the space occupied by the equipment while significantly saving the reagents required for sample processing and reducing the cost of processing reagents required for analysis. It also reduces the amount of waste liquid generated and the cost of waste liquid recovery and processing. Compared with traditional automated processing equipment, it is more economical and environmentally friendly.

[0117] Example 9

[0118] This embodiment is basically the same as the above embodiment, with the following features:

[0119] In this embodiment, see Figure 4 , schematic diagram of the structure of the porous plate transfer mechanism used.

[0120] The sample processing platform 35 also includes an oscillation module 354; the oscillation module 354 is installed on the sample processing platform 35; the oscillation module 354 is provided with a perforated plate seat 3541, a mounting frame 3542, a drive motor 3543 and an eccentric block 3544, the perforated plate seat 3541 is installed on the sample processing platform 35, the mounting frame 3542 is connected to the perforated plate seat 3541, the drive motor 3543 is installed on the mounting frame 3542, and the eccentric block 3544 is installed on the rotating shaft of the drive motor 3543.

[0121] On the sample processing platform 35, the constant temperature culture module 34 is arranged near the edge of one side of the sample processing platform 35, the pipette tip 353 is arranged near the edge of the other side of the sample processing platform 35, the waste liquid tank 352, the pipette tip 353, and the dispersion chamber 39 are located in the middle area of ​​the sample processing platform 35, and the sample processing reagent container 351 is arranged near the placement position of the pipette tip 353.

[0122] Preferably, the dispersion chamber 39 is located in the middle area of ​​the sample processing platform 35 .

[0123] A scanning module 6 is also provided on one side of the dispersion chamber 39. The scanning module 6 is used to scan and obtain a two-dimensional code or a bar code on the slide rack 381 placed in the groove.

[0124] An ultraviolet lamp is also provided above the sample processing platform 35 for sterilization and disinfection.

[0125] Example 10

[0126] This embodiment is basically the same as the above embodiment, with the following features:

[0127] In this embodiment, see Figure 5 , Figure 6 , schematic diagram of the structure of the porous plate transfer mechanism used.

[0128] The dispersion chamber 39 includes a cylinder shell 391 and a turntable 392. The turntable 392 is arranged on the top of the cylinder shell 391. A heating module 393, a humidifying module 394 and a refrigeration and dehumidification module 395 are arranged in the cylinder shell 391. The heating module 393, the humidifying module 394 and the refrigeration and dehumidification module 395 are all located below the turntable 392. The heating module 393, the humidifying module 394 and the refrigeration and dehumidification module 395 are all provided with fans, and a groove is provided on the upper surface of the turntable 392.

[0129] The dispersion chamber 39 is used to control the temperature and humidity during the drop-drip process to facilitate the dispersion of chromosomes.

[0130] Embodiment 11

[0131] The high-throughput cell sample integrated processing device can achieve high throughput and high efficiency of chromosome preparation. The following are the steps for using the high-throughput cell sample integrated processing device to process peripheral blood samples:

[0132] S1. Sample loading: Place the well plate containing the sample into the constant temperature culture module of the equipment and enter the barcode of the well plate;

[0133] S2. Reagent preparation: Add the prepared synchronization solution, autumn water, hypotonic solution, pre-fixation solution and fixation solution into the designated reagent tank;

[0134] S3. Method confirmation: Select or set a suitable method for sample processing;

[0135] S4, start the harvesting process: start the sample processing process by pressing the operation key of the processing process control device;

[0136] S5, the multi-channel pipetting module is loaded with a pipette tip, and the synchronization solution A is added to the sample of the multi-well plate, and the sample is mixed and processed for 12 to 20 hours;

[0137] S6, the multi-channel pipetting module is loaded with a pipette tip, and synchronized solution B is added to the sample in the multi-well plate, and the sample is mixed and processed for a certain time, 3 to 6 hours;

[0138] S7, the multi-channel pipetting module is loaded with a pipette tip, and autumn water is added to the sample of the multi-well plate, and the sample is mixed and processed for 10 to 60 minutes;

[0139] S8, the electric gripper automatically transfers the multi-well plate containing the cultured cells to the sample basket of the centrifuge mechanism, and centrifuges the multi-well plate sample under the control of the program. After the centrifugation is completed, the gripper module clamps the multi-well plate to the moving base bracket of the constant temperature culture module;

[0140] S9, the multi-channel pipetting module is equipped with a pipette tip to absorb the supernatant of the multi-well plate;

[0141] S10, the multi-channel pipetting module is loaded with a pipette tip, and the hypotonic solution is added to the sample in the multi-well plate, and the sample is mixed and processed for 10 to 60 minutes;

[0142] S11, the electric gripper automatically transfers the multi-well plate containing the cultured cells to the sample basket of the centrifuge mechanism, and centrifuges the multi-well plate sample under the control of the program. After the centrifugation is completed, the gripper module clamps the multi-well plate to the moving base bracket of the constant temperature culture module;

[0143] S12, the multi-channel pipetting module is equipped with a pipette tip to absorb the supernatant of the multi-well plate;

[0144] S13, the multi-channel pipetting module is loaded with a pipette tip, and the pre-fixing solution is added to the sample of the multi-well plate, and the sample is mixed and processed for 0 to 5 minutes;

[0145] S14, the electric gripper automatically transfers the multi-well plate containing the cultured cells to the sample basket of the centrifuge mechanism, and centrifuges the multi-well plate sample under the control of the program. After the centrifugation is completed, the gripper module clamps the multi-well plate to the moving base bracket of the constant temperature culture module;

[0146] S15, the multi-channel pipetting module is equipped with a pipette tip to absorb the supernatant of the multi-well plate;

[0147] S16, the multi-channel pipetting module is loaded with a pipette tip, and the fixative is added to the sample in the multi-well plate, and the sample is mixed and then processed for 0 to 5 minutes;

[0148] S17, the electric gripper automatically transfers the multi-well plate containing the cultured cells to the sample basket of the centrifuge mechanism, and centrifuges the multi-well plate sample under the control of the program. After the centrifugation is completed, the gripper module clamps the multi-well plate to the moving base bracket of the constant temperature culture module;

[0149] S18, the multi-channel pipetting module is equipped with a pipette tip to absorb the supernatant of the multi-well plate;

[0150] S19, the multi-channel pipetting module is equipped with a pipette tip, and the fixative is added to the sample in the multi-well plate, and the sample is mixed and treated for 0 to 5 minutes;

[0151] S20, the electric gripper automatically transfers the multi-well plate containing the cultured cells to the sample basket of the centrifuge mechanism, and centrifuges the multi-well plate sample under the control of the program. After the centrifugation is completed, the gripper module clamps the multi-well plate to the motion base bracket of the constant temperature culture module;

[0152] S21, the multi-channel pipetting module is equipped with a pipette tip to absorb the supernatant of the multi-well plate;

[0153] S22, the multi-channel pipetting module is equipped with a pipette tip, and the fixative is added to the sample in the multi-well plate, and the sample is mixed and treated for 0 to 5 minutes;

[0154] S23, the electric gripper automatically transfers the multi-well plate containing the cultured cells to the sample basket of the centrifuge mechanism, and centrifuges the multi-well plate sample under the control of the program. After the centrifugation is completed, the gripper module clamps the multi-well plate to the moving base bracket of the constant temperature culture module;

[0155] S24, slide preparation: print the QR code on the slide and place it on the slide rack;

[0156] S25, the slide suction robot sucks the slide rack and places it in the dispersion chamber;

[0157] S26, the code scanning module performs code scanning comparison;

[0158] S27, the multi-channel pipetting module is equipped with a pipette tip, the supernatant of the multi-well plate is sucked, and the supernatant is dropped onto a glass slide;

[0159] S28, the slide suction robot arm sucks the dripped slide rack and places it into the slide bin;

[0160] S29. During the experiment, the exhaust gas adsorption treatment module can be turned on for adsorption;

[0161] S30. After the experiment, you can turn on the ultraviolet light for disinfection.

[0162] In summary, the high-throughput cell sample integrated processing equipment includes a centrifugal mechanism, a multi-well plate transfer mechanism, a multi-channel pipetting mechanism, a constant temperature culture module, a sample processing platform, a glass slide suction robot arm, a support mechanism and a glass slide bin, and a dispersion chamber. The centrifugal mechanism includes a driving mechanism and a rotating seat, on which a sample hanging basket for placing the multi-well plate for centrifugal operation is installed, which is used to centrifuge the samples in the multi-well plate; the multi-well plate transfer mechanism includes a three-dimensional motion mechanism and a multi-well plate clamping mechanism, the three-dimensional motion mechanism is used to drive the multi-well plate clamping mechanism to perform three-dimensional motion on the multi-well plate, and the multi-well plate clamping mechanism is used to grab the multi-well plate; the multi-channel pipetting mechanism includes a three-dimensional motion mechanism and a pipetting module, the three-dimensional motion mechanism drives the pipetting module to move, and the pipetting module is composed of a pipetting module with 1 to 96 channels, which is used to add reagents to and remove reagents from the multi-well plate; the constant temperature culture module includes a temperature control module, a multi-well plate placement cavity and a motion module. The base is mainly used for constant temperature treatment and cultivation of samples in the multi-well plate; the sample processing platform is provided with a carrying area for placing or installing pipette tips, sample processing reagent containers, waste liquid tanks and constant temperature cultivation modules, glass slide warehouses, and dispersion chambers; the glass slide warehouse is arranged on one side of the dispersion chamber, the glass slide warehouse serves as a placement component for the glass slide rack, the glass slide rack fixes the slide, and the dispersion chamber is provided with a plurality of grooves for fixing the glass slide rack. By setting the slide and the dispersion chamber, the processed samples can be automatically dripped into the slide in batches for analysis. By setting the harvesting and dropping parameters, the samples can be harvested in batches and the slide preparation can be processed, so that the batch analysis of the samples can be integrated, thereby improving the sample processing throughput and sample processing efficiency.

[0163] The present application automatically completes the cycle synchronization, suspension, separation, fixation and dispersion of metaphase cells. Centrifugation, supernatant aspiration, mixing, liquid addition, temperature and humidity control and chromosome dispersion during the process of metaphase cell harvesting are achieved through a pre-set program. The present application can realize the integration of harvesting and slicing, save machine space, replace centrifuge tubes for slicing with well plates, increase slicing throughput, and save reagent usage.

[0164] The embodiments of the utility model are described above in conjunction with the accompanying drawings, but the utility model is not limited to the above embodiments, and various changes can be made according to the purpose of the invention of the utility model. Any changes, modifications, substitutions, combinations or simplifications made according to the spirit and principle of the technical solution of the utility model should be equivalent replacement methods. As long as they meet the purpose of the invention of the utility model and do not deviate from the technical principles and inventive concepts of the utility model, they belong to the protection scope of the utility model.

Claims

1. A high-throughput cell sample integrated processing device, characterized in that: It includes an equipment cabin, a processing flow control device and an equipment function module, wherein the processing flow control device is connected to the equipment function module by signal, the processing flow control device is arranged outside the equipment cabin, and the equipment function module is fixedly installed inside the equipment cabin; The functional modules of the equipment include a centrifugal mechanism, a multi-well plate transfer mechanism, a multi-channel pipetting mechanism, a single-channel pipetting mechanism, a constant temperature culture module, a sample processing platform, a glass slide suction mechanical arm, a support mechanism, a glass slide warehouse and a dispersion chamber; The sample processing platform is arranged at the bottom of the inner cavity of the equipment cabin; the support mechanism is fixedly installed inside the equipment cabin, and the support mechanism is arranged parallel to the sample processing platform; The fixed parts of the multi-well plate transfer mechanism and the multi-channel pipetting mechanism are installed and fixed above the sample processing platform through the supporting mechanism; the sample processing platform is provided with a carrying area for placing or installing a pipetting tip, a sample processing reagent container, a waste liquid tank and a constant temperature culture module, a slide chamber, and a dispersion chamber; The centrifugal mechanism and the dispersion chamber are arranged below the sample processing platform, and the sample processing platform is provided with a first through hole for fixing the centrifugal mechanism and a second through hole for fixing the dispersion chamber, the centrifugal mechanism is fixed in the first through hole, and the dispersion chamber is fixed in the second through hole; The multi-well plate is transferred within the range between the constant temperature culture module and the centrifugal mechanism by a multi-well plate transfer mechanism; the multi-well plate transfer mechanism is located above the sample processing platform; the multi-well plate transfer mechanism is provided with a gripping structure as a working component for taking and placing the multi-well plate; The multi-channel pipetting mechanism is located above the sample processing platform; the multi-channel pipetting mechanism serves as a liquid adding mechanism to automatically add the processing reagent in the sample processing reagent container to the multi-well plate; the multi-channel pipetting mechanism also serves as a liquid suction mechanism to automatically remove the sample from the multi-well plate; The single-channel pipetting mechanism is located above the sample processing platform; the single-channel pipetting mechanism acts as a pipetting mechanism to automatically transfer the sample in the multi-well plate to the surface of the glass slide to realize the sample preparation operation; The constant temperature culture module is located on the table of the sample processing platform and serves as a temperature control device for performing constant temperature treatment on the samples in the multi-well plate; The slide chamber is located on the table of the sample processing platform, and the slide chamber is arranged on one side of the dispersion chamber. The slide chamber serves as a placement component for a slide rack, and the slide rack fixes the slides; The dispersion chamber is provided with a plurality of grooves for fixing the glass slide rack; The slide suction mechanical arm is installed and fixed above the sample processing platform through the supporting mechanism; the slide suction mechanical arm is provided with a grasping structure as a working component for taking and placing the slide rack; The processing flow control device is electrically connected to the centrifugal mechanism, the multi-well plate transfer mechanism, the multi-channel pipetting mechanism, the constant temperature culture module, and the slide suction mechanical arm.

2. The high-throughput cell sample integrated processing device according to claim 1, characterized in that: The sample processing platform also includes an oscillation module; the oscillation module is installed on the sample processing platform; the oscillation module is provided with a perforated plate seat, a mounting frame, a drive motor and an eccentric block, the mounting frame is installed on the sample processing platform, the mounting frame is connected to the perforated plate seat, the drive motor is installed on the mounting frame, and the eccentric block is installed on the rotating shaft of the drive motor.

3. The high-throughput cell sample integrated processing device according to claim 1, characterized in that: The dispersion chamber includes a barrel shell and a turntable, the turntable is arranged on the top of the barrel shell, a heating module, a humidification module and a refrigeration and dehumidification module are arranged in the barrel shell, the heating module, the humidification module and the refrigeration and dehumidification module are all located below the turntable, the heating module, the humidification module and the refrigeration and dehumidification module are all provided with a fan, and the upper surface of the turntable is provided with the groove.

4. The high-throughput cell sample integrated processing device according to claim 1, characterized in that: The high-throughput cell sample integrated processing equipment also includes a waste gas adsorption processing module; the waste gas adsorption processing module is arranged on the top of the equipment cabin, and the waste gas adsorption processing module serves as an absorption and transportation device for the gas in the equipment cabin; the waste gas adsorption processing module includes a waste gas external exhaust fan, a waste gas adsorbent and a fan outer frame fixing frame; the waste gas external exhaust fan is installed on the top of the equipment cabin through the fan outer frame fixing frame; the waste gas adsorbent is arranged below the waste gas external exhaust fan.

5. The high-throughput cell sample integrated processing device according to claim 1, characterized in that: The centrifugal mechanism includes a centrifugal chamber, a driving mechanism and a sample hanging basket. The centrifugal chamber is a closed structure. The centrifugal process of the sample is carried out in the centrifugal chamber. The sample hanging basket is used to place a porous plate with samples to be processed; the driving mechanism includes a centrifugal motor, a motor driver and a transmission mechanism; the centrifugal motor is located at the lower part of the centrifugal mechanism, and the power output end of the centrifugal motor is connected to the sample hanging basket through the transmission mechanism; the centrifugal motor is driven and controlled by the motor driver, and the processing flow control device controls the centrifugal motor through the motor driver to perform centrifugal operations on the samples in the porous plate on the sample hanging basket.

6. The high-throughput cell sample integrated processing device according to claim 1, characterized in that: The multi-porous plate transfer mechanism includes a first three-dimensional motion mechanism and a multi-porous plate clamping mechanism; the first three-dimensional motion mechanism is used to drive the multi-porous plate clamping mechanism to perform three-dimensional motion on the multi-porous plate, and the multi-porous plate clamping mechanism is used to grab the multi-porous plate so that the grabbed multi-porous plate can be operated between the centrifugal mechanism, the constant temperature culture module and the sample processing platform.

7. The high-throughput cell sample integrated processing device according to claim 1, characterized in that: The multi-channel pipetting mechanism includes a second three-dimensional motion mechanism and a multi-channel pipetting module; the second three-dimensional motion mechanism drives the multi-channel pipetting module to move, and the multi-channel pipetting module is used to add reagents to the multi-well plate or absorb reagents; the number of pipetting channels of the multi-channel pipetting module is consistent with the number of holes of the multi-well plate; the multi-channel pipetting module is a 1-96 channel pipetting module.

8. The high-throughput cell sample integrated processing device according to claim 1, characterized in that: The slide suction robot includes a third three-dimensional motion mechanism and a slide rack clamping mechanism; the third three-dimensional motion mechanism is used to drive the slide rack clamping mechanism to perform three-dimensional motion on the slide rack, and the slide rack clamping mechanism is used to grab the slide rack so that the grabbed slide rack can move between the constant temperature culture module, the slide bin, and the dispersion chamber.

9. The high-throughput cell sample integrated processing device according to claim 1, characterized in that: The constant temperature culture module includes a temperature control module, a porous plate placement cavity and a moving base bracket; the temperature control module is composed of a temperature sensor and a temperature control element, and is used to control the temperature inside the porous plate placement cavity; the porous plate placement cavity is a closed cavity with an insulating material on the outside, and is used to place the porous plate containing the processed samples; the moving base bracket is composed of a porous plate base, a traction motor and a motion mechanism, and is used to operate the porous plate containing the processed samples inside and outside the porous plate placement cavity, and to enable the moving base bracket to perform horizontal pulling and transmission inside and outside the porous plate placement cavity, so as to realize the constant temperature treatment of the samples and the transportation operation of the porous plates.

10. The high-throughput cell sample integrated processing device according to claim 1, characterized in that: On the sample processing platform, the constant temperature culture module is arranged near the edge of one side of the sample processing platform, the pipette tip is arranged near the edge of the other side of the sample processing platform, the waste liquid tank, the pipette tip, and the dispersion chamber are located in the middle area of ​​the sample processing platform, and the sample processing reagent container is arranged near the placement position of the pipette tip.

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