Procalcitonin and interleukin 6 joint detection kit

Abstract

The utility model discloses a procalcitonin and interleukin 6 joint detection kit which comprises a kit body and a cover body, an observation opening is formed in the top of one end of the kit body, a sample adding opening is formed in the top of one end, far away from the observation opening, of the kit body, and a detection card containing a fluorescein labeled conjugate of a procalcitonin antibody and an interleukin 6 antibody is installed in the kit body. The detection card contains a fluorescein labeled conjugate containing a procalcitonin antibody and an interleukin antibody, the sample adding port is communicated with the detection card, a slot is formed in one side, close to the box body, of the cover body, and one end, close to the sample adding port, of the box body can be inserted into the slot. By arranging the cover body which can be clamped on the box body, the cover body can effectively protect the sample adding opening from being polluted, and the purity of a blood sample is guaranteed; by arranging the heating bag, after the cover body is opened, the heating bag is in contact with the outside to generate heat, so that a blood sample stably reacts with the detection card, and meanwhile, as the heating bag only heats for a certain time, a timing effect can be achieved.

Description

Technical Field

[0001] The utility model relates to the field of detection kit equipment, in particular to a procalcitonin and interleukin 6 combined detection kit. Background Art

[0002] The procalcitonin / interleukin-6 combined detection reagent is used to detect the content of procalcitonin (PCT) in human samples. It is mainly used in clinical practice for auxiliary diagnosis of bacterial infectious diseases. Dynamic observation of interleukin-6 (IL-6) levels helps to understand the progression of the disease and confirm the efficacy. At the same time, interleukin-6 is also an excellent marker for evaluating the prognosis of patients with sepsis.

[0003] The patent document with the Chinese patent announcement number CN210323044U has an authorization announcement date of April 14, 2020. It discloses a time-resolved detection card and kit for combined detection of IL-6 / PCT. The application utilizes the sensitivity of time-resolved fluorescence immunochromatography technology, rare earth vanadate nanofluorescent labeling materials, and combines with dry immunofluorescence analyzer to realize high-sensitivity, accurate quantitative, simple and fast IL-6 / PCT combined detection time-resolved detection card and kit. The utility model detection card and kit can accurately and quantitatively detect the content of interleukin-6 and procalcitonin in human serum, plasma and whole blood samples. The sample can be processed without pretreatment and dilution of diluent by blood filter membrane sample pad. The time-resolved fluorescence microsphere detection technology can avoid the fluorescence interference of the sample itself. The rare earth vanadate nanomaterial has the advantages of low background, strong fluorescence signal, and high signal-to-noise ratio. The label connects the rare earth vanadate nanoparticles to the antibody through a covalent bond, and the labeling product is stable, with the characteristics of wide detection range, high sensitivity, high accuracy, and fast and simple detection.

[0004] It can be seen from the above application that the existing procalcitonin / interleukin 6 combined detection kit has poor protection. After the sample contacts the detection card, it is not protected in time, and the sample addition port is still exposed to the outside world, which may cause the sample to be contaminated, and the blood sample is easy to coagulate near the sample addition port, which reduces the reaction rate between the blood and the detection card. Therefore, a procalcitonin and interleukin 6 combined detection kit is proposed. Utility Model Content

[0005] The utility model aims to provide a procalcitonin and interleukin 6 combined detection kit. By arranging a cover body that can be snapped on the box body, the cover body can effectively protect the sample addition port from being contaminated, thereby ensuring the purity of the blood sample. By arranging a heating bag, after opening the cover body, the heating bag contacts the outside world to generate heat, so that the blood sample and the detection card react stably. At the same time, because the heating of the heating bag only lasts for a certain period of time, it can play a timing role.

[0006] In order to achieve the above-mentioned purpose, the main technical scheme adopted by the utility model is: a procalcitonin and interleukin 6 combined detection kit, comprising: a box body and a cover body, an observation port is provided at the top of one end of the box body, a sample addition port is provided at the top of the box body away from the observation port, the two ends of the box body are different in height, the side of the box body close to the sample addition port is lower, a detection card containing a fluorescein-labeled conjugate of procalcitonin antibody and interleukin 6 antibody is installed inside the box body, the sample addition port is connected to the detection card, and a slot is opened on the side of the cover body close to the box body, and the end of the box body close to the sample addition port can be inserted into the slot.

[0007] Preferably, a card block is integrally formed on the top of the box body, and the card block is on a side close to the sample loading port. A protruding block is provided on the surface of the card block, a card slot is provided in the slot, and a groove corresponding to the protruding block is provided in the card slot, and the card block can be inserted into the card slot accordingly.

[0008] Preferably, a plurality of positioning blocks are integrally formed on the inner wall of the box body, and the detection card can be clamped between the plurality of positioning blocks.

[0009] Preferably, a heating bag is placed between the plurality of positioning blocks.

[0010] Preferably, a clamping plate is integrally formed on the top of the positioning block, and the clamping plate is clamped on the top of the heating bag.

[0011] Preferably, both sides of the box body are provided with slide grooves, and both sides of the inner wall of the cover body are integrally formed with limiting blocks, the limiting blocks are at one end of the cover body close to the box body, and the limiting blocks slide in the slide grooves.

[0012] Preferably, an anti-dropout block is integrally formed in the slide groove, and the anti-dropout block is located on a side of the slide groove close to the cover body.

[0013] Preferably, the connection between the box body and the cover body is an arc-shaped structure, one side of the box body is a concave arc, and one side of the cover body is a concave arc.

[0014] Preferably, a first gripping groove is provided on the surface of the box body, and the first gripping groove is provided at the top and the bottom of the box body, and an arc-shaped anti-slip pattern is integrally formed in the first gripping groove.

[0015] Preferably, second gripping grooves are provided on both sides of the cover body, and longitudinal anti-slip grooves are integrally formed in the second gripping grooves.

[0016] Compared with the prior art, the beneficial effects of the utility model are:

[0017] The procalcitonin and interleukin-6 combined detection kit is provided with a cover body that can be snapped onto the box body, and the cover body can effectively protect the sample addition port from contamination, thereby ensuring the purity of the blood sample; and a heating bag is provided, and after the cover body is opened, the heating bag is in contact with the outside world to generate heat, so that the blood sample and the detection card react stably. At the same time, because the heating of the heating bag only lasts for a certain period of time, it can play a timing role. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 It is a three-dimensional schematic diagram of the procalcitonin and interleukin-6 combined detection kit of the utility model;

[0019] Figure 2 It is a three-dimensional schematic diagram of the separation of the box body and the cover body of the procalcitonin and interleukin 6 combined detection kit of the utility model;

[0020] Figure 3 It is a side cross-sectional schematic diagram of the procalcitonin and interleukin 6 combined detection kit of the utility model;

[0021] Figure 4 for Figure 3 A magnified view of part A;

[0022] Figure 5 for Figure 3 A magnified view of part B;

[0023] Figure 6 It is a cross-sectional schematic diagram of the logical relationship between the slideway and the limit block in the procalcitonin and interleukin 6 combined detection kit of the utility model.

[0024] In the figure: 1. box body; 101. positioning block; 102. card plate; 11. observation port; 12. sample adding port; 13. card block; 14. first grasping groove; 15. slide groove; 151. anti-dropping card block; 16. heating bag; 161. air hole; 2. detection card; 3. cover body; 31. slot; 311. card slot; 312. limit block; 32. second grasping groove. DETAILED DESCRIPTION

[0025] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0026] See also Figure 1-Figure 6The present embodiment provides a procalcitonin and interleukin 6 combined detection kit, comprising: a box body 1 and a cover body 3, an observation port 11 is provided on the top of one end of the box body 1, a sample addition port 12 is provided on the top of the end of the box body 1 away from the observation port 11, the two ends of the box body 1 are different in height, and the side of the box body 1 close to the sample addition port 12 is lower, a detection card 2 is installed inside the box body 1, and the detection card 2 contains a fluorescent labeled conjugate of procalcitonin antibody and interleukin 6 antibody, and the inner wall of the box body 1 is integrally formed with a plurality of positioning blocks 101, and the detection card 2 can be clamped between the plurality of positioning blocks 101 to prevent the detection card 2 from shaking inside the box body 1, thereby avoiding the detection result If the detection kit is used incorrectly, a heating bag 16 is placed between several positioning blocks 101. The heating bag reacts chemically when it meets air and starts to release heat. An air hole 161 is opened at the connection between the box body 1 and the cover body 3. The air hole 161 connects the inside and outside of the box body 1. When the detection kit is used without unpacking, the cover body 3 is adhered to the air hole 161. When unpacking, the cover body 3 is pulled out to open the air hole 161, so that the heating bag will generate heat when it meets air during use. A card plate 102 is integrally formed on the top of the positioning block 101. The card plate 102 is clamped on the top of the heating bag 16 to fix the position of the heating bag 16. The sample port 12 is connected to the detection card 2;

[0027] A slot 31 is provided on one side of the cover 3 close to the box body 1, and one end of the box body 1 close to the sample injection port 12 can be inserted into the slot 31. A card block 13 is integrally formed on the top of the box body 1. The card block 13 is on the side close to the sample injection port 12. A protruding block is provided on the surface of the card block 13. A card slot 311 is provided in the slot 31, and a groove corresponding to the protruding block is provided in the card slot 311. The card block 13 can be inserted into the card slot 311 accordingly, so that the cover 3 can be tightly clamped on the box body 1 to prevent the cover 3 from falling off. Both sides of the box body 1 are A slide groove 15 is provided, and limiting blocks 312 are integrally formed on both sides of the inner wall of the cover body 3. The limiting blocks 312 are at one end of the cover body 3 close to the box body 1. The limiting blocks 312 slide in the slide groove 15, and an anti-dropping block 151 is integrally formed in the slide groove 15. The anti-dropping block 151 is at one side of the slide groove 15 close to the cover body 3 to prevent the cover body 3 from falling off the box body 1. The connection between the box body 1 and the cover body 3 is an arc structure, one side of the box body 1 is a concave arc, and one side of the cover body 3 is a concave arc. The cover body 3 with a concave arc structure is convenient for exposing the sample addition port 12.

[0028] Structural principle:

[0029] like Figure 1-Figure 6 As shown, when in use, the cover body 3 is pulled out, and the air hole 161 is opened at the same time. The heating bag 16 starts an oxidation reaction and releases heat when it encounters air, so that the sample addition port 12 is exposed, and a blood sample is dripped into the sample addition port 12. Then the cover body 3 is closed, and the blood sample contacts and reacts with the test card 2. After a period of time, the test result will appear at the observation port 11.

[0030] In summary, the present procalcitonin and interleukin 6 combined detection kit is provided with a cover body 3 that can be snapped onto the box body 1, and the cover body 3 can effectively protect the sample addition port 12 from contamination, thereby ensuring the purity of the blood sample; by providing a heating bag 16, after opening the cover body 3, the heating bag 16 is in contact with the outside world to generate heat, so that the blood sample and the detection card 2 react stably. At the same time, because the heating of the heating bag 16 only lasts for a certain period of time, it can play a timing role.

[0031] Parts not involved in the present invention are the same as the prior art or can be implemented by the prior art. Although the embodiments of the present invention have been shown and described, it is understood by those skilled in the art that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principles and spirit of the present invention, and the scope of the present invention is defined by the attached claims and their equivalents.

Claims

1. A procalcitonin and interleukin-6 combined detection kit, characterized in that: include: A box body (1) and a cover body (3), wherein an observation port (11) is provided at the top of one end of the box body (1), and a sample injection port (12) is provided at the top of the end of the box body (1) away from the observation port (11). The two ends of the box body (1) are of different heights, and the side of the box body (1) close to the sample injection port (12) is lower. A detection card (2) containing a fluorescein-labeled conjugate of procalcitonin antibody and interleukin 6 antibody is installed inside the box body (1), and the sample injection port (12) is connected to the detection card (2). A slot (31) is provided on the side of the cover body (3) close to the box body (1), and the end of the box body (1) close to the sample injection port (12) can be inserted into the slot (31); A card block (13) is integrally formed on the top of the box body (1), the card block (13) is on a side close to the sample injection port (12), a protruding block is provided on the surface of the card block (13), a card slot (311) is provided in the slot (311), a groove corresponding to the protruding block is provided in the card slot (311), and the card block (13) can be correspondingly inserted into the card slot (311); The inner wall of the box body (1) is integrally formed with a plurality of positioning blocks (101); the detection card (2) can be clamped between the plurality of positioning blocks (101); a heating bag (16) is placed between the plurality of positioning blocks (101); a clamping plate (102) is integrally formed on the top of the positioning block (101); and the clamping plate (102) is clamped on the top of the heating bag (16).

2. A procalcitonin and interleukin-6 combined detection kit according to claim 1, characterized in that: Slide grooves (15) are provided on both sides of the box body (1); and limiting blocks (312) are integrally formed on both sides of the inner wall of the cover body (3); the limiting blocks (312) are located at one end of the cover body (3) close to the box body (1); and the limiting blocks (312) slide in the slide grooves (15).

3. A procalcitonin and interleukin-6 combined detection kit according to claim 2, characterized in that: An anti-dropout block (151) is integrally formed in the slide groove (15), and the anti-dropout block (151) is located on a side of the slide groove (15) close to the cover body (3).

4. A procalcitonin and interleukin-6 combined detection kit according to claim 3, characterized in that: The connection between the box body (1) and the cover body (3) is an arc-shaped structure, one side of the box body (1) is a concave arc, and one side of the cover body (3) is a concave arc.

5. A procalcitonin and interleukin-6 combined detection kit according to claim 4, characterized in that: The surface of the box body (1) is provided with a first gripping groove (14), the first gripping groove (14) is provided at the top and the bottom of the box body (1), and an arc-shaped anti-slip pattern is integrally formed in the first gripping groove (14).

6. A procalcitonin and interleukin-6 combined detection kit according to claim 5, characterized in that: Second gripping grooves (32) are provided on both sides of the cover body (3), and longitudinal anti-slip grooves are integrally formed in the second gripping grooves (32).

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