ECL reagent preparation reaction device

Abstract

The utility model discloses an ECL reagent preparation reaction device, which comprises a reaction box, a reaction cavity, a reaction cavity and a reaction cavity, the top of the reaction box is open, a box cover is configured, and the reaction box and the box cover are both made of shading materials; the first reaction area is located in the box body of the reaction box, and a reaction film is arranged in the first reaction area; the second reaction area is located in the box body of the reaction box, a flow channel is arranged between the second reaction area and the first reaction area, and the second reaction area and the first reaction area are communicated and connected through the flow channel; and the switch assembly is arranged on the flow channel, and the switch assembly is configured to be used for cutting off / conducting the flow channel. According to the utility model, the influence of external sunlight irradiation on the property of the ECL working solution can be reduced, and the loss of the CL working solution transferred to the reaction film can be reduced.

Description

Technical Field

[0001] The utility model relates to the technical field of experimental equipment, in particular to an ECL reagent configuration reaction device. Background Art

[0002] ECL reagent, namely chemiluminescent horseradish peroxidase substrate, is currently the most sensitive reagent in Western blotting detection. In practice, the configuration of ECL reagent includes the following steps: 1. Perform conventional SDS-PAGE, transfer and Western Blot steps. During the process: label IgG with HRP or use the primary antibody-streptavidin-biotin-HRP clamp method. 2. Take equal volumes of reaction solution A and reaction solution B respectively, put them into a clean container and mix them, and react to generate ECL working solution; 3. After the last wash of Western Blot, take out the reaction membrane with tweezers, absorb the excess washing solution with filter paper and place it in a plastic container. 4. Transfer the ECL working solution in the clean container to the reaction membrane placed in the plastic container through a pipette, and place it for 1-3 minutes to allow the secondary antibody on the reaction membrane to develop color. The problem with the above scheme is that during the reaction process of steps 2 and 4, external sunlight exposure will cause the properties of the ECL working solution to change. In addition, the process of transferring the ECL working solution into the plastic container using a pipette is prone to cause the loss of the ECL working solution. Therefore, how to develop a new ECL reagent configuration reaction device to overcome the above-mentioned problems in the prior art is a direction that technical personnel in this field need to further study. Utility Model Content

[0003] The utility model aims to provide an ECL reagent configuration reaction device, which can reduce the influence of external sunlight on the properties of ECL working solution and reduce the loss of CL working solution when it is transferred to a reaction membrane.

[0004] The utility model discloses an ECL reagent configuration reaction device, comprising:

[0005] A reaction box, the top of which is open and provided with a box cover, wherein both the reaction box and the box cover are made of light-shielding material;

[0006] A first reaction area, the first reaction area is located in the box body of the reaction box, and a reaction membrane is arranged in the first reaction area;

[0007] A second reaction area, the second reaction area is located in the box body of the reaction box, a flow channel is provided between the second reaction area and the first reaction area, and the second reaction area is conductively connected with the first reaction area through the flow channel;

[0008] A switch component is disposed on the flow channel and is configured to cut off / conduct the flow channel.

[0009] By adopting this technical solution: when preparing the ECL reagent, first open the box cover, and pour the A reaction solution (luminol) and the B reaction solution (hydrogen peroxide) into the second reaction area through the top opening of the reaction box; close the box cover to allow the A reaction solution and the B reaction solution to react in a lightless environment to form the ECL working solution. Subsequently, open the box cover, operate the switch assembly to make the flow channel enter the conducting state, so that the ECL working solution in the second reaction area flows into the first reaction area through the flow channel and contacts the reaction membrane, and close the box cover to make the secondary antibody on the reaction membrane develop color. Thus, the influence of external sunlight on the properties of the ECL working solution is reduced, and the loss caused by the transfer of the ECL working solution between different containers is reduced.

[0010] Preferably, the second reaction area is arranged in the cylindrical cavity in the reaction box; a U-shaped notch is arranged on the side wall of the cylindrical cavity, and a slot extending in the vertical direction is respectively arranged on both sides of the U-shaped notch; the U-shaped notch constitutes the flow channel.

[0011] The switch assembly comprises a switch baffle, the shape of which is configured to match the U-shaped notch, and two side edges of the switch baffle are respectively connected with clamping strips, the shape of which is configured to match the clamping slot.

[0012] By adopting this technical solution, the reaction liquid A and the reaction liquid B react in the columnar cavity to form an ECL working liquid. After the reaction is completed, the closing baffle is pulled out from the U-shaped notch, so that the ECL working liquid in the columnar cavity flows through the U-shaped notch to the outside of the columnar cavity and contacts the reaction membrane.

[0013] A preferred solution is: the reaction box is a rectangular box body, including a bottom wall and four side walls; the cylindrical cavity is a rectangular hollow column, and the cylindrical cavity is surrounded by the bottom wall, any one of the four side walls, and a first baffle, a second baffle, and a third baffle connected vertically in sequence; the U-shaped notch is located on any one of the first baffle, the second baffle, and the third baffle.

[0014] Another preferred embodiment is: the reaction box is a rectangular box body, including a bottom wall and four side walls; the cylindrical cavity is a rectangular hollow column, and the cylindrical cavity is surrounded by the bottom wall, any two of the four side walls connected to each other, and a first baffle and a second baffle connected vertically to each other; the U-shaped notch is located on the plate body of the first baffle or the second baffle.

[0015] More preferably, a partition plate is provided in the box body of the reaction box, and the partition plate is configured to divide the inside of the box body of the reaction box into at least two reaction zones, and each of the reaction zones includes the first reaction area and the second reaction area.

[0016] By adopting this technical solution, different ECL reagents can be configured in each reaction zone respectively, thereby realizing the synchronous configuration of multiple groups of different ECL reagents and improving work efficiency.

[0017] Compared with the prior art, the utility model has the following advantages:

[0018] Firstly, the utility model can reduce the influence of external sunlight on the properties of the ECL working solution, and reduce the loss of the CL working solution when it is transferred to the reaction membrane.

[0019] Secondly, the utility model can realize the synchronous configuration of multiple groups of different ECL reagents.

[0020] Finally, the utility model has a simple structure, a convenient operation process, and is easy to prepare and use. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 This is a schematic diagram of the structure of Example 1.

[0022] Figure 2 for Figure 1 Schematic diagram of the structure after removing the box cover.

[0023] Figure 3 for Figure 2 The first partial enlargement.

[0024] Figure 4 for Figure 3 Schematic diagram of the structure after removing the switch baffle.

[0025] Figure 5 This is a schematic diagram of the structure of Example 2, in which the box cover is omitted.

[0026] Figure 6 This is a schematic diagram of the structure of Example 3, in which the box cover is omitted.

[0027] In the figure, the component names corresponding to the reference numerals are as follows:

[0028] 100, reaction box; 200, switch assembly; 300, box cover; 111, reaction membrane; 110, first reaction area; 120, second reaction area; 121, first baffle; 122, second baffle; 123, third baffle; 130, partition plate; 140, slot; 210, switch baffle; 211, convex edge. DETAILED DESCRIPTION

[0029] The following is an explanation of the implementation of the present invention through specific examples, and those skilled in the art can easily understand other advantages and effects of the present invention from the contents disclosed in this specification. The present invention can also be implemented or applied through other different specific implementations, and the details in this specification can also be modified or changed in various ways based on different viewpoints and applications without departing from the spirit of the present invention.

[0030] Example 1, please refer to Figure 1-4 :

[0031] An ECL reagent configuration reaction device, comprising:

[0032] The reaction box 100 has an opening at the top and is provided with a corresponding box cover 300 . Both the reaction box 100 and the box cover 300 are made of light-shielding materials.

[0033] The reaction box 100 is provided with a first reaction area 110 and a second reaction area 120. The first reaction area 110 is used to place a reaction membrane 111. The second reaction area 120 is used to realize the reaction process of forming an ECL working solution from a reaction liquid A and a reaction liquid B. A flow channel is provided between the second reaction area 120 and the first reaction area 110, and the flow channel is conductively connected; a switch component 200 is provided on the flow channel, and the switch component 200 is configured to cut off / conduct the flow channel.

[0034] Specifically, in this example: the reaction box 100 is a rectangular box body, and the reaction box 100 includes a bottom wall and four side walls.

[0035] The second reaction area 120 is a cylindrical cavity disposed in the reaction box 100. The cylindrical cavity is a rectangular hollow column with an open top. The cylindrical cavity is surrounded by the bottom wall, any two of the four side walls connected to each other, and the first baffle 121 and the second baffle 122 connected vertically to each other. That is, one end of the plate body of the first baffle 121 is vertically connected to one side wall, the other end of the plate body of the first baffle 121 is vertically connected to one end of the plate body of the second baffle 122, and the other end of the plate body of the second baffle 122 is vertically connected to another side wall.

[0036] The plate body of the first baffle 121 is provided with a U-shaped notch, the U-shaped notch includes an arc-shaped bottom edge constituting the flow channel, and a slot 140 extending in the vertical direction is respectively provided on both sides of the U-shaped notch. The switch assembly 200 includes a switch baffle 210, the shape of which is configured to match the U-shaped notch, and the two sides of the switch baffle 210 are respectively connected with a clamping strip, and the shape of the clamping strip is configured to match the clamping slot 140. That is, the two clamping strips can be inserted into the two clamping slots 140 synchronously and slide up and down in the vertical direction. The top of the switch baffle 210 is also provided with a convex edge 211 for holding. The switch baffle 210 is configured such that when the two clamping strips slide synchronously along the two clamping slots 140 until the lower end surface of the convex edge 211 abuts against the upper end surface of the first baffle 121, the switch baffle 210 blocks the left and right sides of the U-shaped notch.

[0037] The first reaction area 110 is a space in the box body located outside the cylindrical cavity, and the reaction membrane 111 is laid on the bottom of the first reaction area 110 .

[0038] In practice, the working process is as follows: when preparing the ECL reagent, first open the box cover 300, pour the A reaction solution (luminol) and the B reaction solution (hydrogen peroxide) into the second reaction area 120 (i.e., the columnar cavity) through the top opening of the reaction box 100, and cover the box cover 300 to allow the A reaction solution and the B reaction solution to react in a lightless environment to form the ECL working solution. Subsequently, open the box cover 300, and pull the switch baffle 210 upward from the U-shaped notch, so that the two sides of the U-shaped notch lose the shielding of the switch baffle 210 and are connected, and the ECL working solution flows out of the columnar cavity through the U-shaped notch and contacts the reaction membrane 111, and cover the box cover 300 to allow the secondary antibody on the reaction membrane 111 to develop color. In this way, the influence of external sunlight on the properties of the ECL working solution is reduced, and the loss of the ECL working solution during transfer is reduced.

[0039] Example 2, please refer to Figure 5 :

[0040] The difference between Example 2 and Example 1 is that in this example:

[0041] The columnar cavity is surrounded by the bottom wall, any one of the four side walls, and a first baffle 121 , a second baffle 122 , and a third baffle 123 that are vertically connected in sequence; the U-shaped notch is located on the first baffle 121 .

[0042] Example 3, please refer to Figure 6 :

[0043] The difference between this example and Example 1 is that in this example:

[0044] A partition plate 130 is provided in the reaction box 100, and the partition plate 130 is configured to divide the inside of the reaction box 100 into two reaction zones, each of which includes the first reaction area and the second reaction area. Compared with Example 1, the staff can configure different ECL reagents in the two reaction zones, thereby improving work efficiency.

[0045] The above is a detailed description of the embodiments of the present invention in conjunction with the accompanying drawings, but the present invention is not limited to the above embodiments. Even if various changes are made to the present invention, if these changes fall within the scope of the claims of the present invention and their equivalents, they still fall within the protection scope of the present invention.

Claims

1. An ECL reagent configuration reaction device, characterized in that: include: A reaction box, the top of which is open and provided with a box cover, wherein the reaction box and the box cover are both made of light-shielding material; A first reaction area, the first reaction area is located in the box body of the reaction box, and a reaction membrane is arranged in the first reaction area; A second reaction area, the second reaction area is located in the box body of the reaction box, a flow channel is provided between the second reaction area and the first reaction area, and the second reaction area is conductively connected with the first reaction area through the flow channel; A switch component is disposed on the flow channel and is configured to cut off / conduct the flow channel.

2. The ECL reagent configuration reaction device according to claim 1, characterized in that: The second reaction area is arranged in the cylindrical cavity in the reaction box; a U-shaped notch is arranged on the side wall of the cylindrical cavity, and a slot extending in a vertical direction is respectively arranged on both sides of the U-shaped notch; The switch assembly comprises a switch baffle, the shape of which is configured to match the U-shaped notch, and two side edges of the switch baffle are respectively connected with clamping strips, the shape of which is configured to match the clamping slot.

3. The ECL reagent configuration reaction device according to claim 2, characterized in that: The reaction box is a rectangular box body, including a bottom wall and four side walls; the cylindrical cavity is a rectangular hollow column, and the cylindrical cavity is surrounded by the bottom wall, any one of the four side walls, and a first baffle, a second baffle, and a third baffle connected vertically in sequence; the U-shaped notch is located on any one of the first baffle, the second baffle, and the third baffle.

4. The ECL reagent configuration reaction device according to claim 2, characterized in that: The reaction box is a rectangular box body, including a bottom wall and four side walls; the cylindrical cavity is a rectangular hollow column, and the cylindrical cavity is surrounded by the bottom wall, any two of the four side walls connected to each other, and a first baffle and a second baffle connected vertically to each other; the U-shaped notch is located on the plate body of the first baffle or the second baffle.

5. The ECL reagent configuration reaction device according to any one of claims 1 to 4, characterized in that: A partition plate is disposed in the box body of the reaction box, and the partition plate is configured to divide the inside of the box body of the reaction box into at least two reaction zones, each of which includes the first reaction area and the second reaction area.

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