Prefabricated glue box structure for agarose electrophoresis
By designing a pre-made gel box structure of agarose electrophoresis including positioning columns, combing devices and cutting devices, the problem of inaccurate sample loading and cutting size in agarose electrophoresis is solved, and higher electrophoretic separation accuracy is achieved.
Patent Information
- Application Number
- CN202420595080.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-03-26
- Publication Date
- 2025-03-07
- Estimated Expiration
- 2034-03-26
AI Technical Summary
In agarose electrophoresis, the size of the loading holes is inaccurate and the size of the gel is inaccurate, resulting in poor electrophoretic separation.
A pre-made rubber box structure of agarose electrophoresis is designed, including a rubber box body, positioning column, combing device and cutting device. The comb blade device cooperates with the rubber box body through the positioning column to ensure the consistency of the insertion depth of the comb blade; the cutting device achieves precise cutting through the positioning column and the scale.
Through this design, the accuracy of sample loading holes and cutting size is ensured, and the accuracy and reliability of electrophoretic separation are improved.
Smart Images
Figure CN222580005U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of agarose electrophoresis, in particular to a prefabricated gel box structure for agarose electrophoresis. Background Art
[0002] Agarose electrophoresis is a method of electrophoresis that uses agar or agarose as a supporting medium. For samples with larger molecular weights, such as macromolecular nucleic acids and viruses, agarose gels with larger pore sizes can generally be used for electrophoresis separation.
[0003] At present, most laboratories use agarose gels that are prepared on site. The main preparation method includes weighing agarose, adding electrophoresis buffer and heating until completely dissolved; adding ethidium bromide and cooling in a 65°C water bath; preparing a gel mold, placing a suitable comb on the gel plate to form loading holes in the gel, pouring the dissolved agarose gel into the gel plate, solidifying at room temperature, removing the comb, cutting the gel into the size required, and taking out the gel. This agarose gel can be directly placed in the electrophoresis tank for electrophoresis.
[0004] In actual operation, multiple combs need to be placed, and the combs are often tilted when placed. After the comb is inserted into the gel and released, the gel shrinks and pushes the comb out a little, resulting in inaccurate sample hole size. When cutting the gel, it is necessary to measure first and then cut, and the cutting size is inaccurate. Utility Model Content
[0005] 1. Technical issues to be solved
[0006] The technical problem to be solved by the utility model is that the size of the sample loading hole is inaccurate and the size of the gel cutting is inaccurate.
[0007] (II) Technical solution
[0008] In order to solve the above technical problems, the technical solution provided by the utility model is as follows: an agarose electrophoresis prefabricated gel box structure comprises a gel box body, and positioning columns are fixed at the top corners of the gel box body;
[0009] A combing device is provided above the plastic box body, and the combing device includes a plate body sleeved on the positioning column, and a plurality of combing blades extending into the plastic box body are evenly provided at the bottom of the plate body;
[0010] A cutting device is provided above the plastic box body, and the cutting device includes a square frame sleeved on the positioning column, a symmetrically arranged slide groove is provided on the inner side of the square frame, a slide plate is provided in the slide groove, and a cutting knife is provided on the slide plate that penetrates the slide plate and extends into the plastic box body.
[0011] As an improvement, a plurality of limiting holes cooperating with the positioning columns are evenly arranged on the plate body.
[0012] As an improvement, a plurality of second limiting holes cooperating with the positioning posts are evenly arranged on the square frame.
[0013] As an improvement, the slide is provided with a through groove arranged along the length direction of the slide, and the cutting knife is arranged in the through groove.
[0014] As an improvement, the cutter comprises a blade arranged in the through slot, and a handle is provided on the top of the blade.
[0015] As an improvement, the square frame is provided with a scale matching the through slot.
[0016] (III) Beneficial effects
[0017] Compared with the prior art, the utility model has the following advantages: 1. The combing device cooperates with the positioning column on the gel box body, so that multiple combing blades can be inserted into the gel at one time, and the insertion depth of the combing blades is the same without tilting. 2. The positioning column can position the cutting device, and the slide and the scale cooperate to make the slice cutting more accurate. BRIEF DESCRIPTION OF THE DRAWINGS
[0018] Figure 1 It is a structural schematic diagram of the combing device of the utility model.
[0019] Figure 2 It is a structural schematic diagram of the combing device of the utility model in a side view.
[0020] Figure 3 This utility model Figure 2 Schematic diagram of the structure in cross-section at AA.
[0021] Figure 4 It is a structural schematic diagram of the cutting device of the utility model.
[0022] Figure 5 It is a schematic diagram of the structure of the cutting device of the utility model in a top view.
[0023] Figure 6 This utility model Figure 5 Schematic diagram of the structure in cross-section at BB.
[0024] As shown in the figure: 1. Plastic box body; 2. Positioning column; 3. Combing device; 4. Plate body; 5. Combing; 6. Cutting device; 7. Frame; 8. Slide; 9. Slide; 10. Cutter; 11. Limiting hole 1; 12. Limiting hole 2; 13. Through slot; 14. Blade; 15. Handle; 16. Ruler. DETAILED DESCRIPTION
[0025] The embodiments of the present invention are described in detail below, and examples of the embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are intended to be used to explain the present invention, and should not be construed as limiting the present invention.
[0026] In the description of the present invention: It should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "clockwise", "counterclockwise" and the like indicate directions or positional relationships based on the directions or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the referred device or element must have a specific direction, be constructed and operated in a specific direction, and therefore should not be understood as a limitation on the present invention.
[0027] In addition, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include one or more of the features. In the description of the present utility model, unless otherwise specified, "plurality" means two or more, unless otherwise clearly defined.
[0028] In the present invention, unless otherwise clearly specified and limited, the terms "install", "connect", "connect", "fix" and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be an indirect connection through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.
[0029] In the present utility model, unless otherwise clearly specified and limited, a first feature being "above" or "below" a second feature may include that the first and second features are in direct contact, or may include that the first and second features are not in direct contact but are in contact through another feature between them. Moreover, a first feature being "above", "above" and "above" a second feature includes that the first feature is directly above and obliquely above the second feature, or simply indicates that the first feature is higher in level than the second feature. A first feature being "below", "below" and "below" a second feature includes that the first feature is directly below and obliquely below the second feature, or simply indicates that the first feature is lower in level than the second feature.
[0030] Embodiment 1
[0031] Combined with Figure 1-6 The agarose electrophoresis prefabricated gel box structure comprises a gel box body 1, and positioning columns 2 are fixed at the top corners of the gel box body 1 to position the installation of the combing device 3 and the cutting device 6;
[0032] Combined with Figure 1-3 A combing device 3 is provided above the gel box body 1, and the combing device 3 includes a plate body 4 sleeved on the positioning column 2, and a plurality of combing pieces 5 extending into the gel box body 1 are evenly provided at the bottom of the plate body 4, and a plurality of limiting holes 11 cooperating with the positioning column 2 are evenly provided on the plate body 4. The limiting holes 11 on the plate body 4 are sleeved on the positioning column 2, and the plate body 4 is pressed downward to insert the combing pieces 5 into the gel, and the combing device 3 can be removed after waiting for a while;
[0033] Combined with Figure 4-6 A cutting device 6 is provided above the plastic box body 1, and the cutting device 6 includes a square frame 7 sleeved on the positioning column 2, and a symmetrically arranged slide groove 8 is provided on the inner side of the square frame 7, and a slide plate 9 is provided in the slide plate 8. The slide plate 9 is provided with a cutter 10 that penetrates the slide plate 9 and extends into the plastic box body 1. The square frame 7 is evenly provided with a plurality of limiting holes 12 that cooperate with the positioning column 2. The square frame 7 can be sleeved on the positioning column 2 to fix the cutting device 6. The slide plate 9 is provided with a plurality of limiting holes 12 that cooperate with the positioning column 2. The square frame 7 can be sleeved on the positioning column 2 to fix the cutting device 6. A through slot 13 is provided along the length direction of the slide 9, and the cutter 10 is provided in the through slot 13. The cutter 10 includes a blade 14 provided in the through slot 13, and a handle 15 is provided on the top of the blade 14. A scale 16 matching the through slot 13 is provided on the frame 7. The slide 9 is moved to a suitable size, and the scale 16 is aligned with the through slot 13 to obtain a precise size. The cutter 10 is inserted into the through slot 13 and moved along the through slot 13 to accurately cut the gel.
[0034] All features described in the specification, the attached claims and the drawings, whether alone or in any combination thereof, are important features of the present utility model.
[0035] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "an implementation", "specific implementation", "other implementations", "example", "specific example" or "some examples" means that the specific features, structures, materials or characteristics described in combination with the embodiment or example are included in at least one embodiment, implementation or example of the utility model. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described above may also be combined in a suitable manner in any one or more embodiments, implementations or examples. The technical solution recorded in the utility model also includes a technical solution formed by any one or more of the specific features, structures, materials or characteristics described above in a separate or combined manner.
[0036] Although the embodiments of the utility model have been shown and described above, it can be understood that the above embodiments are exemplary and cannot be understood as limiting the utility model. Ordinary technicians in the field can change, modify, replace, modify, delete some features, add features or re-combine features to form a technical solution in the above embodiments without departing from the principles and purpose of the utility model within the scope of the utility model. Any simple modifications, equivalent changes and modifications made to the above embodiments based on the innovative principles of the utility model are still within the scope of the technical solution of the utility model.
Claims
1. Agarose electrophoresis prefabricated gel box structure, including a gel box body, characterized in that: Positioning columns are fixedly arranged at the top corners of the plastic box body; A combing device is provided above the plastic box body, and the combing device includes a plate body sleeved on the positioning column, and a plurality of combing blades extending into the plastic box body are evenly provided at the bottom of the plate body; A cutting device is provided above the plastic box body, and the cutting device includes a square frame sleeved on the positioning column, a symmetrically arranged slide groove is provided on the inner side of the square frame, a slide plate is provided in the slide groove, and a cutting knife is provided on the slide plate that penetrates the slide plate and extends into the plastic box body.
2. The agarose electrophoresis precast gel box structure according to claim 1, characterized in that: The plate body is evenly provided with a plurality of limiting holes 1 cooperating with the positioning columns.
3. The agarose electrophoresis precast gel cassette structure according to claim 1, characterized in that: The square frame is evenly provided with a plurality of limiting holes 2 which cooperate with the positioning columns.
4. The agarose electrophoresis precast gel cassette structure according to claim 1, characterized in that: The slide is provided with a through slot arranged along the length direction of the slide, and the cutter is arranged in the through slot.
5. The agarose electrophoresis precast gel box structure according to claim 4, characterized in that: The cutter comprises a blade arranged in the through slot, and a handle is arranged on the top of the blade.
6. The agarose electrophoresis precast gel box structure according to claim 4, characterized in that: The square frame is provided with a scale matched with the through slot.