Constant-temperature water bath box

By designing a multi-layer storage unit and water circulation system in a constant temperature water bath, the problem of low efficiency of existing constant temperature water baths is solved, and more efficient cellular harvesting and uniform distribution of constant temperature water is achieved.

CN222586633UActive Publication Date: 2025-03-11SHAOXING WOMEN & CHILDRENS HOSPITAL
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Patent Information

Application Number
CN202420577276.5
Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
Filing Date
2024-03-25
Publication Date
2025-03-11
Estimated Expiration
2034-03-25

AI Technical Summary

Technical Problem

The existing constant temperature water bath is inefficient during the harvesting of amniotic fluid cells because it can only lay the culture bottles flat, resulting in only a small number of culture bottles being placed at a time.

Method used

A constant temperature water bath is designed, with a multi-layer storage unit installed inside the box, and a constant temperature water is installed inside each storage unit. The culture bottle is placed on the water surface, and the water circulation and uniform distribution are achieved through the liquid outlet pipe and the diversion tank.

Benefits of technology

Through the design of the multi-layer storage unit, the number of culture bottles placed in the box at one time is improved, the efficiency of cell harvest is improved, and the uniform distribution of constant temperature water and the consistency of water level is ensured through the setting of the diversion tank and the outlet hole.

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Abstract

The utility model discloses a constant-temperature water bath box which comprises a box body and a constant-temperature water circulating device, the constant-temperature water circulating device can circulate water in the box body, a cover body covers the upper part of the box body, a first support frame is fixed in the box body, a plurality of storage units are placed on the upper part of the first support frame, and the plurality of storage units are arranged in an up-and-down overlapping manner; the storage unit comprises a storage box, a second supporting frame is installed in the storage box, and culture bottles can be placed on the upper portion of the second supporting frame. The multiple layers of storage units are arranged in the box body, the constant-temperature water is arranged in each layer of storage unit, and the culture bottles are placed on the surface of the constant-temperature water, so that the efficiency is improved.
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Description

Technical Field

[0001] The utility model relates to the technical field of cell culture equipment, and more specifically, to a constant temperature water bath. Background Art

[0002] During the process of amniotic fluid cell harvesting, pancreatic enzyme digestion solution needs to be injected into the culture bottle where the cells are grown, and then placed in a 37-degree constant temperature water bath for 7 minutes, and the cells attached to the wall are detached with pancreatic enzyme digestion solution. In order to effectively ensure the maximization of pancreatic enzyme digestion, the culture bottle needs to be placed horizontally on the water surface, that is, the surface of the culture bottle is close to the water surface, so the culture bottle can only be laid flat in the constant temperature water bath, so that only a small number of culture bottles can be placed in the constant temperature water bath at a time, which is very inefficient, so there is an urgent need to improve this. Utility Model Content

[0003] The purpose of the utility model is to overcome the deficiencies of the above-mentioned prior art and provide a constant temperature water bath, by arranging multiple layers of storage units inside the box body, each layer of storage units is provided with constant temperature water, and culture bottles are placed on the surface of the constant temperature water, thereby improving efficiency.

[0004] In order to achieve the above purpose, the utility model adopts the following technical solutions:

[0005] The utility model discloses a constant temperature water bath, comprising a box body and a constant temperature water circulation device, wherein the constant temperature water circulation device can circulate water inside the box body, and the upper part of the box body is covered with a cover body. The utility model is characterized in that a first support frame is fixed inside the box body, a plurality of storage units are placed on the upper part of the first support frame, and the plurality of storage units are arranged in an upper and lower stacking manner, and the storage unit comprises a storage box, a second support frame is installed inside the storage box, and a culture bottle can be placed on the upper part of the second support frame.

[0006] Furthermore, a liquid outlet pipe is provided inside the box body, and water in the liquid outlet pipe can flow into the storage unit located at the top, and water in the storage unit located at the top can flow into the storage unit located at the bottom.

[0007] Furthermore, handles are connected to both sides of the storage box, a guide groove is provided between the handle and the side wall of the storage box, and a liquid inlet hole is provided on the side of the storage box, and the liquid inlet hole is connected to the guide groove and the inside of the storage box respectively.

[0008] Furthermore, a liquid outlet is provided on the same side of the storage box and the handle, and the position of the liquid outlet is lower than the liquid inlet, so that water can enter the storage box from the liquid inlet and then flow out from the liquid outlet.

[0009] Furthermore, a conversion tube is connected to the upper portion of the liquid outlet pipe, and the conversion tube includes a connector and a water outlet head, the connector is connected to the liquid outlet pipe, and the water outlet head is located above the guide groove.

[0010] Furthermore, the position of the liquid outlet is higher than the upper surface of the second support frame for placing the culture bottle.

[0011] Furthermore, a boss is provided on the upper portion of the storage box, and a groove is provided on the lower portion of the storage box. The boss of the storage box located adjacent to each other and located below can be inserted into the groove of the storage box located above for limiting position.

[0012] The beneficial effects of the utility model are:

[0013] The utility model can greatly increase the number of culture bottles that can be placed in the box at one time by arranging multi-level storage units, which is beneficial to improving the efficiency of cell harvesting. By arranging a handle, it is not only convenient to take out the storage unit, but also can form a guide groove between the storage box and the side wall to facilitate the diversion of constant temperature water. By arranging a liquid outlet, it can effectively ensure that the water level in each storage box remains consistent. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 is a cross-sectional view of this embodiment;

[0015] Figure 2 for Figure 1 A magnified view at point A;

[0016] Figure 3 for Figure 1 Enlarged view at B;

[0017] Figure 4 A cross-sectional view of a storage unit.

[0018] Figure numerals: 1. Box body; 11. Constant temperature water circulation device; 12. Liquid outlet pipe; 13. First support frame; 2. Cover body; 3. Conversion tube; 31. Connector; 32. Water outlet; 4. Storage unit; 5. Storage box; 51. Handle; 52. Guide groove; 53. Liquid inlet hole; 54. Liquid outlet hole; 55. Boss; 56. Groove; 6. Second support frame; 7. Culture bottle. DETAILED DESCRIPTION

[0019] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0020] like Figures 1 to 4As shown, a constant temperature water bath comprises a box body 1 and a constant temperature water circulation device 11. The constant temperature water circulation device 11 can circulate the water inside the box body 1. The upper part of the box body 1 is covered with a cover body 2. The constant temperature water circulation device 11 is a prior art. After the water inside the box body 1 enters the constant temperature water circulation device 11, it is heated or cooled to a set temperature value by the constant temperature water circulation device 11 and then transported into the box body 1 again to realize constant temperature water circulation.

[0021] A first support frame 13 is fixed inside the box body 1, and a plurality of storage units 4 are placed on the upper part of the first support frame 13. The plurality of storage units 4 are arranged in an upper and lower overlapped manner. The storage units 4 include a storage box 5, and a second support frame 6 is installed inside the storage box 5. A culture bottle 7 can be placed on the upper part of the second support frame 6. A liquid outlet pipe 12 is provided inside the box body 1, and water in the liquid outlet pipe 12 can flow into the storage unit 4 located at the top, and water in the storage unit 4 located at the top can flow into the storage unit 4 located below. Handles 51 are connected to both sides of the storage box 5, and a guide groove 52 is provided between the handle 51 and the side wall of the storage box 5. A liquid inlet hole 53 is provided on the side of the storage box 5, and the liquid inlet hole 53 is connected to the guide groove 52 and the inside of the storage box 5 respectively. A liquid outlet hole 54 is provided on the same side of the storage box 5 and the handle 51, and the position of the liquid outlet hole 54 is lower than the liquid inlet hole 53, so that water can enter the storage box 5 from the liquid inlet hole 53 and then flow out from the liquid outlet hole 54.

[0022] like Figures 1 to 3 As shown, after the culture bottle 7 is placed horizontally on the surface of the second support frame 6 of each storage unit 4, several storage units 4 are stacked and placed inside the box body 1. The constant temperature water circulation device 11 heats the water to 37 degrees Celsius and then flows into the guide groove 52 of the top storage unit 4 from the liquid outlet pipe 12, and flows into the storage box 5 through the liquid inlet hole 53. When the liquid level in the storage box 5 reaches the liquid level c, it flows out from the liquid outlet hole 54. The constant temperature water flowing out of the liquid outlet hole 54 then enters the guide groove 52 of the storage unit 4 located below. Finally, the constant temperature water can flow out from the liquid outlet hole 54 of the bottom storage unit 4 to the bottom of the box body 1 and circulate again through the constant temperature water circulation device 11.

[0023] The utility model can greatly increase the number of culture bottles 7 that can be placed in the box body 1 at one time by providing a multi-level storage unit 4, which is beneficial to improving the efficiency of cell harvesting. By providing a handle 51, it is not only convenient to take out the storage unit 4, but also can form a guide groove 52 between the side wall of the storage box 5 to facilitate the diversion of constant temperature water. By providing a liquid outlet 54, it can effectively ensure that the water level in each storage box 5 remains consistent.

[0024] Among them, the position of the liquid outlet 54 is higher than the upper surface of the second support frame 6 for placing the culture bottle 7, that is, the height of the liquid level c is slightly higher than the upper surface of the second support frame 6. When the culture bottle 7 is placed horizontally on the upper surface of the second support frame 6, the constant temperature water can stick to the side wall of the culture bottle 7.

[0025] The upper part of the liquid outlet pipe 12 is connected to a conversion tube 3, and the conversion tube 3 includes a connector 31 and a water outlet head 32. The connector 31 is connected to the liquid outlet pipe 12, and the water outlet head 32 is located above the guide groove 52. The conversion tube 3 is fixed to the lower part of the cover body 2. When the cover body 2 is opened, the conversion tube 3 is separated from the liquid outlet pipe 12. This design can effectively prevent interference from the conversion tube 3 when taking out the storage unit 4, and the structural design is reliable.

[0026] The storage box 5 is provided with a boss 55 on the upper part, and a groove 56 on the lower part. The boss 55 of the storage box 5 adjacent to each other and located at the lower part can be inserted into the groove 56 of the storage box 5 located at the upper part for limiting. Through the cooperation of the boss 55 and the groove 56, not only the upper and lower overlapping of the storage units 4 can be achieved, but also the upper storage unit 4 can be prevented from tipping over, thereby improving the reliability of the structure.

[0027] The above is only a preferred embodiment of the present invention. The protection scope of the present invention is not limited to the above embodiments. All technical solutions under the concept of the present invention belong to the protection scope of the present invention. It should be pointed out that for ordinary technicians in this technical field, some improvements and modifications without departing from the principle of the present invention should also be regarded as the protection scope of the present invention.

Claims

1. A constant temperature water bath, comprising a housing (1) and a constant temperature water circulation device (11), wherein the constant temperature water circulation device (11) is capable of circulating water inside the housing (1), wherein the upper portion of the housing (1) is covered with a cover (2), wherein: A first support frame (13) is fixed inside the box body (1), and a plurality of storage units (4) are placed on the upper part of the first support frame (13). The plurality of storage units (4) are arranged in an upper and lower stacked manner. The storage unit (4) comprises a storage box (5), and a second support frame (6) is installed inside the storage box (5). A culture bottle (7) can be placed on the upper part of the second support frame (6).

2. A constant temperature water bath according to claim 1, characterized in that: A liquid outlet pipe (12) is provided inside the box body (1), and water in the liquid outlet pipe (12) can flow into the storage unit (4) located at the top, and water in the storage unit (4) located at the top can flow into the storage unit (4) located at the bottom.

3. A constant temperature water bath according to claim 2, characterized in that: Handles (51) are connected to both sides of the storage box (5); a guide groove (52) is provided between the handle (51) and the side wall of the storage box (5); a liquid inlet hole (53) is provided on the side of the storage box (5); the liquid inlet hole (53) is connected to the guide groove (52) and the inside of the storage box (5) respectively.

4. A constant temperature water bath according to claim 3, characterized in that: A liquid outlet (54) is provided on the same side of the storage box (5) and the handle (51). The liquid outlet (54) is located lower than the liquid inlet (53). Water can enter the storage box (5) from the liquid inlet (53) and then flow out from the liquid outlet (54).

5. A constant temperature water bath according to claim 3, characterized in that: The upper part of the liquid outlet pipe (12) is connected to a conversion pipe (3), the conversion pipe (3) comprises a connecting head (31) and a water outlet head (32), the connecting head (31) is connected to the liquid outlet pipe (12), and the water outlet head (32) is located above the guide groove (52).

6. A constant temperature water bath according to claim 4, characterized in that: The position of the liquid outlet hole (54) is higher than the upper surface of the second support frame (6) for placing the culture bottle (7).

7. A constant temperature water bath according to claim 1, characterized in that: The storage box (5) is provided with a boss (55) at the top and a groove (56) at the bottom. The boss (55) of the storage box (5) located adjacent to each other and located below can be inserted into the groove (56) of the storage box (5) located above for limiting position.