Microorganism screening inoculation disc

Abstract

The utility model relates to the technical field of microorganism screening, in particular to a microorganism screening inoculation disc which comprises an inoculation disc body, a containing groove is formed in the top end of the inoculation disc body, a rubber sleeve is bonded to the inner surface of the containing groove, a sample adding cylinder is inserted into the rubber sleeve, and a lap joint ring is fixed to the top end of the sample adding cylinder. A rubber plug is bonded to the middle of the lap joint ring, a check ring is fixed to the edge of the top end of the inoculation disc, and a cover plate covers the top end of the check ring. The microbial sampling device has the beneficial effects that sealed sampling and storage can be carried out on the interior of the sampling barrel, safe screening of microorganisms is kept, and meanwhile, the sampling barrel can accurately sample the microorganisms in a sealed manner and transfer the microorganisms through the needle head, the piston rod and the piston; and the cover plate is detachably positioned, mounted and connected with the inoculation disc through the connecting plate and the positioning pin, so that the inoculation disc is convenient to disassemble, replace and clean.

Description

Technical Field

[0001] The utility model relates to the technical field of microorganism screening, in particular to a microorganism screening inoculation plate. Background Art

[0002] Strain screening is one of the important steps in the production of microbial enzymes. The quality of the screened strains is not only restricted by the strain variety, but also closely related to environmental factors such as temperature, humidity and microorganisms during the sample transfer process. The screening of excellent strains requires ensuring its sealing when sampling, and disinfecting and sterilizing the sample storage environment during the transfer process to avoid contamination of the strains, thereby ensuring the purity and activity of the produced enzymes.

[0003] For example, the prior art Chinese patent publication number "CN212451418U" provides a strain inoculation plate for rapid screening, including a strain inoculation plate consisting of a base plate and an upper plate, the base plate is provided with neatly arranged sample loading holes, the upper plate is provided with a hand-held portion, and neatly arranged inoculation columns are provided below the upper plate, the arrangement of the inoculation columns corresponds to the arrangement of the sample loading holes, so that the inoculation columns can extend into the sample loading holes; the inner diameter of the sample loading holes is 4.5-5.0 mm, and the hole depth is 5.0 mm; the diameter of the inoculation columns is 3.0 mm, and the height is 10.0 mm.

[0004] The device can be inoculated with 21 different strains at a time, and can be used for qualitative initial screening and rescreening of microorganisms that produce a certain enzyme. It can also be used for antibacterial spectrum experiments of a certain antibacterial substance or a new antibiotic against a variety of microorganisms. It can be used for qualitative or quantitative experiments on the antibacterial activity of active substances produced by microorganisms using the plate punching method. It can also be used for confrontation culture experimental research between strains, etc. It can greatly reduce the workload and save experimental materials, the consistency of conditions is easy to control, and the results are more reproducible.

[0005] The existing microbial screening inoculation plate is not sealed enough during the sample addition and sampling process, and is easily contaminated and affected by bacteria in the external air, which is not conducive to microbial screening. At the same time, the inoculation plate is relatively fixed, and it is not convenient to disassemble and clean the inoculation plate when changing different microorganisms for screening. Utility Model Content

[0006] The utility model aims to solve the shortcomings of the prior art that the inoculation plate is not sealed enough during the sample addition and sampling process, is easily contaminated and affected by bacteria in the external air, and is not conducive to the screening of microorganisms, and proposes a microorganism screening inoculation plate.

[0007] In order to achieve the above purpose, the utility model provides the following technical solutions:

[0008] A microorganism screening inoculation plate is designed, including an inoculation plate, a placement groove is opened at the top of the inoculation plate, a rubber sleeve is bonded to the inner surface of the placement groove, a sample adding tube is inserted into the rubber sleeve, a lap ring is fixed to the top of the sample adding tube, a rubber plug is bonded to the middle of the lap ring, a retaining ring is fixed to the top edge of the inoculation plate, a cover plate is covered at the top of the retaining ring, a clamping plate is fixed to the top of the cover plate, a protective strip is bonded to the inner side of the clamping plate, a sampling tube is inserted between the clamping plates, a scale bar is engraved on the surface of the sampling tube, a piston is slidably arranged inside the sampling tube, a piston rod is fixed to the top of the piston, a connecting tube is fixed to the bottom end of the sampling tube, a needle is fixed to the bottom end of the connecting tube, a through hole is opened on the surface of the cover plate, and the connecting tube passes through the through hole.

[0009] Furthermore, a connecting plate is fixed to the outer side of the cover plate, a first positioning hole is opened on the surface of the connecting plate, a second positioning hole is opened on the side of the inoculation plate, positioning pins are inserted into the first positioning hole and the second positioning hole, and a limiting plate is fixed to the outer end of the positioning pin.

[0010] Furthermore, a first guide hole is opened at the bottom end of the placement groove, and a second guide hole is opened at the bottom end of the rubber sleeve. A push rod slides through the inside of the first guide hole and the second guide hole, and a push-pull plate is fixed to the bottom end of the push rod. A rubber strip is connected between the top edge of the push-pull plate and the bottom end of the inoculation tray, and a support leg is fixed to the bottom end of the inoculation tray.

[0011] Furthermore, the inoculation plate is a circular structure and is arranged horizontally, and the sample adding cylinder is a cylindrical structure and is arranged with an open top.

[0012] Furthermore, the overlapping ring is overlapped on the top of the inoculation plate, the rubber stopper is a circular structure and is horizontally arranged, the retaining ring is a circular ring structure, and the cover plate is a circular plate structure and is horizontally arranged.

[0013] Furthermore, the clamping plate is a long strip structure and is vertically arranged, the protective strip is clamped on the outside of the sampling tube, the sampling tube is a cylindrical structure and is vertically arranged, the needle passes through the rubber stopper and is inserted into the inner bottom end of the sampling tube.

[0014] Furthermore, the connecting plate is an arc-shaped plate structure and is vertically arranged on the outside of the inoculation plate, the first positioning hole and the second positioning hole are aligned, the positioning pin is horizontally arranged, and the limiting plate is arranged on the outside of the connecting plate.

[0015] Furthermore, the first guide hole and the second guide hole are aligned vertically, the push rod is arranged with its top end abutting against the middle of the bottom end of the sample loading tube, the push-pull plate is a circular plate structure and is arranged horizontally, and the rubber strip is vertically arranged around the push rod.

[0016] The utility model provides a microorganism screening inoculation plate, which has the following beneficial effects:

[0017] 1. The utility model arranges a sample adding cylinder at the top of the inoculation plate, and a rubber plug at the top of the sample adding cylinder, and a cover plate is arranged at the top of the inoculation plate through a retaining ring, and a sampling cylinder is arranged at the top of the cover plate through a clamping plate, and a needle arranged at the bottom end thereof passes through the rubber plug through a connecting tube and is inserted into the interior of the sample adding cylinder, so that the interior of the sample adding cylinder can be sealed for sample addition and storage, and safe screening of microorganisms can be maintained. At the same time, through the needle, piston rod and piston, the sampling cylinder can seal and accurately sample microorganisms and transfer them.

[0018] 2. The sample adding tube of the utility model is arranged on the top of the inoculation tray through a rubber sleeve and a placement groove, and the sample adding tube can be lifted upward by a push-pull plate and a pushing rod, which is convenient for taking, replacing and cleaning the sample adding tube and convenient for screening different microorganisms. The cover plate is detachably positioned and installed with the inoculation tray through a connecting plate and a positioning pin, which is convenient for disassembly, replacement and cleaning of the inoculation tray. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0020] Figure 2 For this utility model Figure 1 A cross-sectional view of

[0021] Figure 3 For this utility model Figure 2 A magnified image of point A;

[0022] Figure 4 For this utility model Figure 2 The enlarged view of point B;

[0023] Figure 5 For this utility model Figure 2 Enlarged view of point C.

[0024] In the figure: 1. inoculation plate; 2. rubber strip; 3. push rod; 4. push-pull plate; 5. support leg; 6. rubber sleeve; 7. sample tube; 8. needle; 9. placement groove; 10. connecting plate; 11. retaining ring; 12. lap ring; 13. clamping plate; 14. sampling tube; 15. piston rod; 16. piston; 17. cover plate; 18. protective strip; 19. rubber plug; 20. scale strip; 21. through hole; 22. connecting tube; 23. second guide hole; 24. first guide hole; 25. second positioning hole; 26. first positioning hole; 27. limit plate; 28. positioning pin. DETAILED DESCRIPTION

[0025] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of the embodiments.

[0026] Example 1

[0027] See also Figure 1 , Figure 2 , Figure 3 , Figure 4 and Figure 5 The microorganism screening inoculation plate shown in the figure includes an inoculation plate 1, a placement groove 9 is opened at the top of the inoculation plate 1, a rubber sleeve 6 is bonded to the inner surface of the placement groove 9, a sample adding tube 7 is inserted into the rubber sleeve 6, a lap ring 12 is fixed to the top of the sample adding tube 7, a rubber plug 19 is bonded to the middle of the lap ring 12, a retaining ring 11 is fixed to the top edge of the inoculation plate 1, a cover plate 17 is covered at the top of the retaining ring 11, and a clamping plate 13 is fixed to the top of the cover plate 17 A protective strip 18 is bonded to the inner side of the clamping plate 13, a sampling barrel 14 is inserted between the clamping plates 13, a scale bar 20 is engraved on the surface of the sampling barrel 14, a piston 16 is slidably arranged inside the sampling barrel 14, a piston rod 15 is fixed to the top of the piston 16, a connecting tube 22 is fixed to the bottom end of the sampling barrel 14, a needle 8 is fixed to the bottom end of the connecting tube 22, a through hole 21 is opened on the surface of the cover plate 17, and the connecting tube 22 passes through the through hole 21.

[0028] A rubber stopper 19 is arranged at the top of the sample adding cylinder 7, and a cover plate 17 is arranged at the top of the inoculation plate 1 through a retaining ring 11. A sampling cylinder 14 is arranged at the top of the cover plate 17 through a clamping plate 13, and a needle 8 arranged at the bottom end thereof passes through the rubber stopper 19 through a connecting tube 22 and is inserted into the interior of the sample adding cylinder 7. Thus, the interior of the sample adding cylinder 7 can be sealed for sample addition and preservation to maintain safe screening of microorganisms. At the same time, through the needle 8, the piston rod 15 and the piston 16, the sampling cylinder 14 can seal and accurately sample microorganisms and transfer them.

[0029] The inoculation plate 1 is a circular structure and is arranged horizontally, and the sample adding cylinder 7 is a cylindrical structure and is arranged with an open top.

[0030] The overlapping ring 12 is overlapped on the top of the inoculation plate 1, the rubber stopper 19 is a circular structure and is horizontally arranged, the retaining ring 11 is a circular ring structure, and the cover plate 17 is a circular plate structure and is horizontally arranged.

[0031] The clamping plate 13 is a long strip structure and is vertically arranged. The protective strip 18 is clamped on the outside of the sampling tube 14. The sampling tube 14 is a cylindrical structure and is vertically arranged. The needle 8 passes through the rubber stopper 19 and is inserted into the inner bottom end of the sample adding tube 7.

[0032] Example 2

[0033] See also Figure 1 , Figure 2 , Figure 3 , Figure 4 and Figure 5 The microorganism screening inoculation plate shown in the figure includes an inoculation plate 1, a placement groove 9 is opened at the top of the inoculation plate 1, a rubber sleeve 6 is bonded to the inner surface of the placement groove 9, a sample adding tube 7 is inserted into the rubber sleeve 6, a lap ring 12 is fixed to the top of the sample adding tube 7, a rubber plug 19 is bonded to the middle of the lap ring 12, a retaining ring 11 is fixed to the top edge of the inoculation plate 1, a cover plate 17 is covered at the top of the retaining ring 11, and a clamping plate 13 is fixed to the top of the cover plate 17 A protective strip 18 is bonded to the inner side of the clamping plate 13, a sampling barrel 14 is inserted between the clamping plates 13, a scale bar 20 is engraved on the surface of the sampling barrel 14, a piston 16 is slidably arranged inside the sampling barrel 14, a piston rod 15 is fixed to the top of the piston 16, a connecting tube 22 is fixed to the bottom end of the sampling barrel 14, a needle 8 is fixed to the bottom end of the connecting tube 22, a through hole 21 is opened on the surface of the cover plate 17, and the connecting tube 22 passes through the through hole 21.

[0034] A rubber stopper 19 is arranged at the top of the sample adding cylinder 7, and a cover plate 17 is arranged at the top of the inoculation plate 1 through a retaining ring 11. A sampling cylinder 14 is arranged at the top of the cover plate 17 through a clamping plate 13, and a needle 8 arranged at the bottom end thereof passes through the rubber stopper 19 through a connecting tube 22 and is inserted into the interior of the sample adding cylinder 7. Thus, the interior of the sample adding cylinder 7 can be sealed for sample addition and preservation to maintain safe screening of microorganisms. At the same time, through the needle 8, the piston rod 15 and the piston 16, the sampling cylinder 14 can seal and accurately sample microorganisms and transfer them.

[0035] A connecting plate 10 is fixed to the outer side of the cover plate 17, a first positioning hole 26 is opened on the surface of the connecting plate 10, a second positioning hole 25 is opened on the side of the inoculation plate 1, positioning pins 28 are inserted into the first positioning hole 26 and the second positioning hole 25, and a limiting plate 27 is fixed to the outer end of the positioning pin 28.

[0036] A first guide hole 24 is formed at the bottom end of the placement groove 9, and a second guide hole 23 is formed at the bottom end of the rubber sleeve 6. A push rod 3 slides through the inside of the first guide hole 24 and the second guide hole 23. A push-pull plate 4 is fixed to the bottom end of the push-pull plate 3. A rubber strip 2 is connected between the top edge of the push-pull plate 4 and the bottom end of the inoculation tray 1. A support leg 5 is fixed to the bottom end of the inoculation tray 1.

[0037] The sample adding tube 7 is arranged on the top of the inoculation tray 1 through the rubber sleeve 6 and the placement groove 9, and the sample adding tube 7 can be lifted upward by the push-pull plate 4 and the pushing rod 3, which is convenient for taking, replacing and cleaning the sample adding tube 7 and convenient for screening different microorganisms. The cover plate 17 is detachably positioned and installed with the inoculation tray 1 through the connecting plate 10 and the positioning pin 28, which is convenient for disassembly, replacement and cleaning of the inoculation tray 1.

[0038] The connecting plate 10 is an arc-shaped plate structure and is vertically arranged on the outside of the inoculation tray 1 . The first positioning hole 26 is aligned with the second positioning hole 25 . The positioning pin 28 is horizontally arranged. The limiting plate 27 is arranged on the outside of the connecting plate 10 .

[0039] The first guide hole 24 and the second guide hole 23 are aligned up and down, the push rod 3 is set, and its top end abuts against the middle of the bottom end of the sample adding tube 7, the push-pull plate 4 is a circular plate structure and is horizontally arranged, and the rubber strip 2 is vertically arranged around the push rod 3.

[0040] Working method: by setting a sample loading cylinder 7 at the top of the inoculation plate 1, and a rubber plug 19 at the top of the sample loading cylinder 7, and the cover plate 17 is set at the top of the inoculation plate 1 through the retaining ring 11, and the sampling cylinder 14 is set at the top of the cover plate 17 through the clamping plate 13, and the needle 8 set at the bottom end thereof passes through the rubber plug 19 through the connecting tube 22 and is inserted into the interior of the sample loading cylinder 7. Therefore, the interior of the sample loading cylinder 7 can be sealed for loading and preservation to maintain safe screening of microorganisms. At the same time, through the needle 8, the piston rod 15 and the piston 16, the sampling cylinder 14 can seal and accurately sample microorganisms and transfer them.

[0041] The sample adding tube 7 is arranged on the top of the inoculation tray 1 through the rubber sleeve 6 and the placement groove 9, and the sample adding tube 7 can be lifted upward by the push-pull plate 4 and the pushing rod 3, which is convenient for taking, replacing and cleaning the sample adding tube 7 and convenient for screening different microorganisms. The cover plate 17 is detachably positioned and installed with the inoculation tray 1 through the connecting plate 10 and the positioning pin 28, which is convenient for disassembly, replacement and cleaning of the inoculation tray 1.

[0042] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes within the technical scope disclosed by the present invention according to the technical scheme and the utility model concept of the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A microorganism screening inoculation plate, comprising an inoculation plate (1), characterized in that: The top of the inoculation tray (1) is provided with a placement groove (9), the inner surface of the placement groove (9) is bonded with a rubber sleeve (6), a sample adding tube (7) is inserted into the rubber sleeve (6), a lap ring (12) is fixed to the top of the sample adding tube (7), a rubber plug (19) is bonded to the middle of the lap ring (12), a retaining ring (11) is fixed to the top edge of the inoculation tray (1), the top of the retaining ring (11) is covered with a cover plate (17), a clamping plate (13) is fixed to the top of the cover plate (17), and the inner side of the clamping plate (13) is fixed to the top of the cover plate (17). A protective strip (18) is bonded thereto, a sampling tube (14) is inserted between the clamping plates (13), a scale bar (20) is engraved on the surface of the sampling tube (14), a piston (16) is slidably arranged inside the sampling tube (14), a piston rod (15) is fixed to the top of the piston (16), a connecting tube (22) is fixed to the bottom end of the sampling tube (14), a needle (8) is fixed to the bottom end of the connecting tube (22), a through hole (21) is opened on the surface of the cover plate (17), and the connecting tube (22) passes through the through hole (21).

2. A microorganism screening inoculation plate according to claim 1, characterized in that: A connecting plate (10) is fixed to the outer side of the cover plate (17), a first positioning hole (26) is formed on the surface of the connecting plate (10), a second positioning hole (25) is formed on the side of the inoculation plate (1), positioning pins (28) are inserted into the first positioning hole (26) and the second positioning hole (25), and a limiting plate (27) is fixed to the outer end of the positioning pin (28).

3. A microorganism screening inoculation plate according to claim 1, characterized in that: A first guide hole (24) is formed at the bottom end of the placement groove (9), and a second guide hole (23) is formed at the bottom end of the rubber sleeve (6). A push rod (3) slides through the inside of the first guide hole (24) and the second guide hole (23). A push-pull plate (4) is fixed to the bottom end of the push-pull plate (3). A rubber strip (2) is connected between the top edge of the push-pull plate (4) and the bottom end of the inoculation tray (1). A support leg (5) is fixed to the bottom end of the inoculation tray (1).

4. A microorganism screening inoculation plate according to claim 1, characterized in that: The inoculation plate (1) is of a circular structure and is arranged horizontally, and the sample adding cylinder (7) is of a cylindrical structure and is arranged with an open top.

5. A microorganism screening inoculation plate according to claim 1, characterized in that: The overlapping ring (12) is overlapped on the top of the inoculation plate (1), the rubber stopper (19) is a circular structure and is arranged horizontally, the retaining ring (11) is a circular ring structure, and the cover plate (17) is a circular plate structure and is arranged horizontally.

6. A microorganism screening inoculation plate according to claim 1, characterized in that: The clamping plate (13) is a long strip structure and is arranged vertically. The protective strip (18) is clamped on the outside of the sampling tube (14). The sampling tube (14) is a cylindrical structure and is arranged vertically. The needle (8) passes through the rubber stopper (19) and is inserted into the inner bottom end of the sample adding tube (7).

7. A microorganism screening inoculation plate according to claim 2, characterized in that: The connecting plate (10) is an arc-shaped plate structure and is vertically arranged on the outside of the inoculation plate (1), the first positioning hole (26) and the second positioning hole (25) are aligned, the positioning pin (28) is horizontally arranged, and the limiting plate (27) is arranged on the outside of the connecting plate (10).

8. A microorganism screening inoculation plate according to claim 3, characterized in that: The first guide hole (24) and the second guide hole (23) are aligned vertically, the push rod (3) is arranged with its top end abutting against the middle of the bottom end of the sample adding cylinder (7), the push-pull plate (4) is a circular plate structure and is arranged horizontally, and the rubber strip (2) is vertically arranged around the push rod (3).

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