Grid type cell culture bottle with breathable top

Abstract

The utility model belongs to the technical field of cell culture, and relates to a grating type cell culture bottle with a breathable top. The grating type top-breathable cell culture bottle comprises a bottom wall, a side wall formed by extending upwards from the bottom wall, and a top wall for sealing the top of the side wall, the top wall comprises a plurality of ventilation parts arranged at intervals, the two ends of each ventilation part are connected with the two opposite side walls, each ventilation part comprises a plurality of ventilation holes formed in the extending direction of the ventilation parts at intervals, and hydrophobic filter membranes are arranged on the ventilation holes in an attached mode. The ventilation part connected with the two side walls is arranged on the top wall, the length of the ventilation part is increased, the ventilation amount is increased, and therefore it is guaranteed that the concentration of gas in the container from top to bottom is uniform. And the air holes in the top wall are arranged in a grating manner, so that the stability of the structure is enhanced. The hydrophobic filter membrane on each air hole effectively prevents liquid and pollutants from invading, and meanwhile, necessary gas exchange is promoted, so that the functionality and the safety of the culture bottle are improved.

Description

Technical Field

[0001] The utility model belongs to the technical field of cell culture and relates to a grid-type top air-permeable cell culture bottle. Background Art

[0002] Cell culture flasks are important tools for cell culture, and are mainly used to culture and observe various cell types in biological laboratories. Cell culture flasks are usually made of transparent plastic or glass materials, which are convenient for observing the growth of cells. They are also equipped with suitable bottle caps to ensure closure or gas exchange needs to maintain a gas concentration suitable for cell growth.

[0003] However, when using traditional cell culture bottles, gas exchange is mainly carried out through the breathable bottle cap at the top. This often leads to uneven distribution of carbon dioxide in the bottle, especially in the bottom area far from the bottle cap, where the carbon dioxide concentration may be far below 5%, which has a significant impact on the growth and differentiation of cells. The growth state of cells will vary significantly depending on the distance from the bottle mouth, ultimately resulting in a loss of reliability in experimental results.

[0004] In order to improve ventilation efficiency and distribute gas as evenly as possible, some cell culture bottles in the prior art have a larger air hole on the top of the bottle, or add additional air holes on the side wall. However, the large air hole on the top of the bottle still fails to effectively solve the problem of low ventilation efficiency in corners and other areas far away from the air hole, and the growth state of cells still varies significantly depending on the distance from the air hole. As for opening air holes on the side wall, because it is necessary to avoid leakage of contamination of the culture solution, the height of the culture solution cannot exceed the height of the air hole, which will affect the capacity of the bottle that can be used to hold the culture solution, limiting the practicality and functionality of the cell culture bottle. Utility Model Content

[0005] The utility model aims to provide a grid-type top-permeable cell culture bottle which can improve the ventilation efficiency and does not limit the capacity of the culture fluid.

[0006] To achieve the above object, the utility model provides the following technical solutions: a grid-type top-permeable cell culture bottle, comprising a bottom wall, a side wall extending upward from the bottom wall, and a top wall closing the top of the side wall;

[0007] The top wall includes a plurality of air permeable portions arranged at intervals, and both ends of each of the air permeable portions are connected to two opposite side walls. Each of the air permeable portions includes a plurality of air holes arranged at intervals along the extension direction of the air permeable portion, and a hydrophobic filter membrane is fitted on the air hole.

[0008] Furthermore, the top wall also includes a plurality of connecting parts, the connecting parts and the ventilating parts are arranged alternately, and the two sides of the top wall are connected to the side walls through the connecting parts.

[0009] Furthermore, the connecting portion and the venting portion are integrally formed, and the hydrophobic filter membrane is arranged on the bottom surface of the top wall and is used to cover all the venting holes.

[0010] Furthermore, the grid-type top-permeable cell culture flask also includes a mounting plate fixed on the side wall, a plurality of ventilation holes corresponding to the ventilation holes are formed on the mounting plate, and the hydrophobic filter membrane is defined between the top of the mounting plate and the top wall.

[0011] Furthermore, the hydrophobic filter membrane is fixed to the air permeable portion, and the air permeable portion to which the hydrophobic filter membrane is fixed is detachably installed between the connecting portions.

[0012] Furthermore, the ventilating portion and the connecting portion are sealed and connected via a sealing strip.

[0013] Furthermore, the air holes are circular or rectangular.

[0014] Furthermore, a pipetting port is provided on the side wall, and a bottle cap is sleeved on the pipetting port.

[0015] The beneficial effects of the utility model are as follows: the present application provides a breathable portion connected to two opposite side walls so that the breathable portion penetrates the top wall, thereby increasing the length of the breathable portion and increasing the ventilation volume, ensuring that the concentrations of carbon dioxide and oxygen in every corner from the top wall to the bottom wall are maintained within an ideal range. In addition, the breathable portion is provided on the top wall, which will not limit the capacity of the culture medium. Secondly, the breathable holes are arranged at intervals to form a grid-like structure, thereby enhancing the structural strength and stability of the top wall. In addition, each breathable hole is equipped with a hydrophobic filter membrane, which effectively prevents the entry of liquids and pollutants while ensuring the necessary gas exchange.

[0016] The above description is only an overview of the technical solution of the utility model. In order to more clearly understand the technical means of the utility model and implement it according to the contents of the specification, the following is a detailed description of the preferred embodiments of the utility model in conjunction with the accompanying drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 This is a schematic structural diagram of a grid-type top-permeable cell culture bottle shown in an embodiment of the utility model;

[0018] Figure 2 It is a schematic diagram of the structure of the ventilation part of the grid-type top ventilation cell culture bottle arranged along the short axis;

[0019] Figure 3 for Figure 1 The cross-sectional view along the AA direction;

[0020] Figure 4 This is a schematic structural diagram of a grid-type top-ventilated cell culture flask shown in another embodiment of the utility model;

[0021] Figure 5 for Figure 4 A schematic diagram of the structure of the middle ventilating part and the connecting part;

[0022] Figure 6 for Figure 4 Another structural schematic diagram of the middle ventilating portion and the connecting portion. DETAILED DESCRIPTION

[0023] The technical solution of the utility model will be described clearly and completely below in conjunction with the accompanying drawings. Obviously, the described embodiments are part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0024] In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicating the orientation or positional relationship, are based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first", "second", and "third" are used for descriptive purposes only, and cannot be understood as indicating or implying relative importance.

[0025] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installation", "connection" and "connection" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0026] In addition, the technical features involved in the different embodiments of the present invention described below can be combined with each other as long as they do not conflict with each other.

[0027] See also Figure 1 and Figure 3A grid-type top-permeable cell culture flask 100 shown in a preferred embodiment of the present application includes a bottom wall 1, a side wall 2 extending upward from the bottom wall 1, and a top wall 3 closing the top of the side wall 2.

[0028] The top wall 3 includes a plurality of air permeable portions 31 spaced apart from each other. Both ends of each air permeable portion 31 are connected to two opposite side walls 2. Each air permeable portion 31 includes a plurality of air holes 311 spaced apart from each other along the extension direction of the air permeable portion 31. A hydrophobic filter membrane 312 is fitted on the air hole 311.

[0029] In this embodiment, the air permeable portion 31 is arranged along the long axis direction of the grid-type top gas permeable cell culture flask 100 to maximize the area covered by the air holes 311, thereby optimizing the gas flow in the entire grid-type top gas permeable cell culture flask 100. The arrangement of the air permeable portion 31 along the long axis direction allows the gas to diffuse more evenly from the top wall 3 to the bottom wall 1, promoting the effective distribution of the gas in the long axis direction. In this way, not only the efficiency of gas exchange is enhanced, but also the uniformity of gas exchange is maintained.

[0030] See also Figure 2 In another embodiment, the air permeable portion 31 is arranged along the short axis direction of the grid-top gas permeable cell culture flask 100, providing a shorter straight path to accelerate the lateral flow of gas in the grid-top gas permeable cell culture flask 100. The air permeable portion 31 arranged in the short axis direction is conducive to quickly balancing the gas concentration, especially when the grid-top gas permeable cell culture flask 100 needs to respond quickly to environmental changes. In addition, compared with the solution of setting along the long axis, the air permeable portion 31 arranged along the short axis on the top wall 3 can avoid the weakening of the structural strength of the top wall 3 due to the large-area opening, thereby enhancing the structural stability of the top wall 3.

[0031] The top wall 3 also includes a plurality of connecting parts 32, which are staggered with the air-permeable parts 31. The two sides of the top wall 3 are connected to the side walls 2 through the connecting parts 32. The connecting parts 32 and the side walls 2 are connected to form a non-porous continuous structure, which enhances the structural strength of the connection between the top wall 3 and the side walls 2. Optionally, the side walls 2 can also be connected to the side walls 2 through the air-permeable parts 31, but this may weaken the structural strength of the grid-type top air-permeable cell culture flask 100, and the connection between the air-permeable parts 31 and the side walls 2 may become a stress concentration point, increasing the risk of damage.

[0032] In one embodiment, the connection portion 32 and the air permeable portion 31 are integrally formed, and the hydrophobic filter membrane 312 is disposed on the bottom surface of the top wall 3 and is used to cover all the air permeable holes 311. Specifically, the top wall 3 is directly formed by injection molding technology, and the connection portion 32 and the air permeable portion 31 are integral, thereby ensuring that the connection between the top wall 3 and the side wall 2 is more solid and durable, and the sealing is guaranteed.

[0033] See also Figure 3 A layer of hydrophobic filter membrane 312 is installed on the bottom surface of the top wall 3. This layer of hydrophobic filter membrane 312 is spread over the bottom surface of the entire top wall 3 and covers all the air holes 311. The main function of the hydrophobic filter membrane 312 is to prevent liquid from entering the interior of the grid-type top gas-permeable cell culture bottle 100 through the air holes 311, and at the same time prevent the invasion of pollutants, ensuring the cleanliness of the internal environment and the aseptic conditions of cell culture. The gas entering through the air holes 311 is filtered by the hydrophobic filter membrane 312, thereby increasing safety and improving the quality of gas exchange.

[0034] The grid-type top-permeable cell culture bottle 100 further includes a mounting plate 21 fixed on the side wall 2, on which a plurality of vents 211 corresponding to the vents 311 are formed, and a hydrophobic filter membrane 312 is defined between the top of the mounting plate 21 and the top wall 3. The vents 211 on the mounting plate 21 are aligned with the vents 311 one by one to ensure unimpeded flow of gas from the vents 311 to the vents 211, further optimizing the gas distribution. As for the two sides of the hydrophobic filter membrane 312, they are mutually supported by the top wall 3 and the mounting plate 21 to define the position of the hydrophobic filter membrane 312 and filter the gas.

[0035] See also Figure 4 In another embodiment, the air permeable portion 31 is fixed with a hydrophobic filter membrane 312 , and the air permeable portion 31 fixed with the hydrophobic filter membrane 312 can be detachably installed between the connecting portions 32 .

[0036] See also Figure 5 Specifically, mounting holes 322 penetrating the top wall 3 are formed between adjacent connecting parts 32, a platform 321 lower than the upper surface of the connecting part 32 is formed on the circumference of the mounting holes 322, and a hanging ear 313 is arranged on the circumference of the air permeable part 31. When the air permeable part 31 is inserted into the mounting holes 322, the hanging ear 313 overlaps the platform 321, thereby completing the installation of the air permeable part 31 and the connecting part 32. As a detachable module, the air permeable part 31 can be easily installed or replaced, and a suitable air permeable part 31 can be selected for different cells.

[0037] See also Figure 6 Furthermore, the mounting hole 322 can be set to a tapered structure with a gradually decreasing cross-section from top to bottom, and the air permeable portion 31 is also set to a tapered structure matching the mounting hole 322, so that the air permeable portion 31 and the mounting hole 322 fit more closely, thereby enhancing the sealing performance.

[0038] Furthermore, the ventilating portion 31 and the connecting portion 32 are sealed and connected by a sealing strip (not shown). After the ventilating portion 31 and the connecting portion 32 are installed, the sealing strip can be used to cover the joint between the ventilating portion 31 and the connecting portion 32 to enhance the sealing performance.

[0039] The hydrophobic filter membrane 312 is fixed to the bottom surface of the air permeable portion 31. Optionally, an additional plate can be provided at the bottom of the air permeable portion 31, and the hydrophobic filter membrane 312 is defined by the plate and the air permeable portion 31. This structure is similar to that of the previous embodiment and will not be described in detail here. Figure 4 Optionally, the hydrophobic filter membrane 312 may be directly disposed in the air hole 311, so that the gas is filtered by the hydrophobic filter membrane 312 while passing through the air hole 311. The hydrophobic filter membrane 312 is fixed in the air hole 311 by bonding. Optionally, it may be fixed by other methods, which are not limited here.

[0040] In this embodiment, the air holes 311 are rectangular. Optionally, the air holes 311 may also be circular or in other shapes, which are not limited here.

[0041] In this embodiment, a pipetting port 22 is provided on the side wall 2 , and a bottle cap 221 is sleeved on the pipetting port 22 . The bottle cap 221 is opened to replace the liquid through the pipetting port 22 .

[0042] The beneficial effects of the present application are as follows: by providing a ventilating portion 31 connected to two opposite side walls 2, the ventilating portion 31 penetrates the top wall 3, thereby increasing the length of the ventilating portion 31, thereby increasing the ventilation volume, and ensuring that the concentrations of carbon dioxide and oxygen in every corner from the top wall 3 to the bottom wall 1 are maintained within an ideal range. Secondly, by providing ventilating holes 311 at intervals, the ventilating holes 311 form a grid-like structure, thereby enhancing the structural strength and stability of the top wall. In addition, each ventilating hole 311 is equipped with a hydrophobic filter membrane 312, which effectively prevents the entry of liquids and pollutants while ensuring the necessary gas exchange.

[0043] The technical features of the above-described embodiments may be arbitrarily combined. To make the description concise, not all possible combinations of the technical features in the above-described embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0044] The above-mentioned embodiments only express several implementation methods of the utility model, and the description is relatively specific and detailed, but it cannot be understood as limiting the scope of the utility model patent. It should be pointed out that for ordinary technicians in this field, several modifications and improvements can be made without departing from the concept of the utility model, which all belong to the protection scope of the utility model. Therefore, the protection scope of the utility model patent shall be based on the attached claims.

Claims

1. A grid-type top-ventilated cell culture flask, characterized in that: It includes a bottom wall, a side wall extending upward from the bottom wall, and a top wall closing the top of the side wall; The top wall includes a plurality of air permeable portions arranged at intervals, and both ends of each of the air permeable portions are connected to two opposite side walls. Each of the air permeable portions includes a plurality of air holes arranged at intervals along the extension direction of the air permeable portion, and a hydrophobic filter membrane is fitted on the air hole.

2. The grid-type top-permeable cell culture flask according to claim 1, characterized in that: The top wall also includes a plurality of connecting parts, the connecting parts and the ventilating parts are arranged alternately, and the two sides of the top wall are connected to the side walls through the connecting parts.

3. The grid-type top gas-permeable cell culture flask according to claim 2, characterized in that: The connecting portion and the venting portion are integrally formed, and the hydrophobic filter membrane is arranged on the bottom surface of the top wall and is used to cover all the venting holes.

4. The grid-type top-permeable cell culture flask according to claim 3, characterized in that: The grid-type top-permeable cell culture bottle also includes a mounting plate fixed on the side wall, a plurality of ventilation holes corresponding to the ventilation holes are formed on the mounting plate, and the hydrophobic filter membrane is defined between the top of the mounting plate and the top wall.

5. The grid-type top-permeable cell culture flask according to claim 2, characterized in that: The hydrophobic filter membrane is fixed to the air permeable portion, and the air permeable portion fixed with the hydrophobic filter membrane can be detachably installed between the connecting portions.

6. The grid-type top-permeable cell culture flask according to claim 5, characterized in that: The ventilating portion and the connecting portion are sealed and connected via a sealing strip.

7. The grid-type top-permeable cell culture flask according to claim 1, characterized in that: The air holes are circular or rectangular.

8. The grid-type top-permeable cell culture flask according to claim 1, characterized in that: The side wall is provided with a transfer port, and the transfer port is sleeved with a bottle cap.

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