Sample fluorescence positioning system

Abstract

The utility model discloses a sample fluorescence positioning system which comprises a liquid carrying table for carrying a detection medium and an imaging piece, the liquid carrying table is provided with an illumination light source for exciting sample fluorescence, and the imaging piece is arranged on the liquid carrying table and is used for receiving sample fluorescence light to record the position of a sample. The method has the advantages that the sample can be accurately and quickly positioned, and the accuracy and efficiency of fluorescence detection are improved.

Description

Technical Field

[0001] The utility model relates to the technical field of fluorescence microscopes, in particular to a sample fluorescence positioning system. Background Art

[0002] Light sheet fluorescence microscopy is an advanced microscopic imaging technology that achieves fast, deep layered tomographic scanning and 3D dynamic imaging of large-volume samples by selectively illuminating biological samples layer by layer. However, in a light sheet fluorescence microscopy system, before performing a three-dimensional scan of the sample, it is necessary to predetermine the approximate position information of the sample, determine the position deviation between the sample and the scanning imaging point, and then calibrate the sample position based on the position deviation, and move the sample to the focal plane of the three-dimensional scan. However, since the sample slices used for light sheet fluorescence microscopy are often highly transparent, traditional bright field illumination cannot achieve clear imaging of the object to be tested and cannot accurately position the sample.

[0003] In view of this, the purpose of the utility model is to provide a new technical solution to solve the existing technical problems. Utility Model Content

[0004] In order to overcome the deficiencies of the prior art, the utility model provides a sample fluorescence positioning system, which solves the problem that the existing bright field illumination cannot accurately position the sample.

[0005] The technical solution adopted by the utility model to solve its technical problems is:

[0006] A sample fluorescence positioning system comprises a liquid carrier for carrying a detection medium and an imaging component, wherein the liquid carrier is provided with an illumination light source for exciting sample fluorescence, the imaging component is arranged on the liquid carrier and is used to receive sample fluorescence light to record the position of the sample.

[0007] In the above structure, a light-transmitting window is provided on the liquid-carrying table, and the illumination light source emits fluorescence from the light-transmitting window toward the sample.

[0008] In the above structure, the light-transmitting window is light-transmitting glass, and the refractive index of the light-transmitting glass is the same as the refractive index of the detection medium.

[0009] In the above structure, the lighting light source is an LED light source.

[0010] In the above structure, the imaging element is an imaging camera or an imaging objective lens.

[0011] The above structure further comprises a control unit, which is electrically connected to the imaging element and is used to receive position information of the imaging element to calibrate the position of the sample.

[0012] The above structure further comprises a translation stage and a driving unit. The translation stage is arranged at one side of the liquid carrier stage. The driving unit is electrically connected to the control unit. The driving unit is used to drive the translation stage to move.

[0013] In the above structure, a connecting boss is provided on the liquid carrying platform, and a bolt hole is opened on the connecting boss.

[0014] The beneficial effect of the utility model is that the fluorescence information of the sample can be stimulated by using an illumination light source with a specific wavelength, and the initial positioning of the sample can be achieved by collecting the fluorescence information. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] The utility model is further described below in conjunction with the accompanying drawings and embodiments.

[0016] Figure 1 It is the principle diagram of the utility model;

[0017] Figure 2 It is a schematic diagram of the overall structure of the utility model;

[0018] Figure 3 It is a top view schematic diagram of the utility model.

[0019] Figure numerals: 1. liquid carrier; 11. light-transmitting window; 12. connecting boss; 2. illumination light source; 3. imaging element; 4. displacement stage; 5. sample. DETAILED DESCRIPTION

[0020] The following is combined with Figure 1-3 The utility model is further described.

[0021] The following will clearly and completely describe the concept, specific structure and technical effects of the utility model in combination with the embodiments and drawings, so as to fully understand the purpose, characteristics and effects of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of them. Based on the embodiments of the utility model, other embodiments obtained by technicians in this field without creative work are all within the scope of protection of the utility model. In addition, all the connection / connection relationships involved in the patent do not refer to the direct connection of components, but refer to the formation of a better connection structure by adding or reducing connection accessories according to the specific implementation situation. The various technical features in the creation of the utility model can be combined interchangeably without conflicting with each other.

[0022] Reference Figures 1 to 3The utility model provides a sample fluorescence positioning system, which is used to position and calibrate the position of a sample 5 before a light sheet fluorescence microscopy system performs three-dimensional scanning, so that the sample 5 is located on the imaging focal plane of the light sheet fluorescence microscopy system, so as to facilitate the subsequent three-dimensional scanning work. The sample fluorescence positioning system includes a liquid carrier 1, an imaging component 3 and an illumination light source 2. The liquid carrier 1 is used to carry a detection medium, such as an ECI reagent, etc.; the illumination light source 2 is used to illuminate the sample 5 to be detected and make the sample 5 emit fluorescence; the imaging component 3 is used to receive the fluorescent light emitted by the sample 5 to record the position information of the sample 5. In the fields of biology, chemistry, etc., fluorescence detection is a commonly used experimental technique. By stimulating the fluorescent substance in the sample 5 to make it emit fluorescence, and then detecting the fluorescence signal, the qualitative and quantitative analysis of the sample 5 is achieved. The utility model can quickly and accurately locate the sample 5 by stimulating the fluorescence of the sample 5, collecting the fluorescence information of the sample 5 to determine the position information of the sample 5, and calibrating the position of the sample 5 using the obtained position information.

[0023] Reference Figure 2 and Figure 3 Furthermore, a light-transmitting window 11 is provided on the liquid-carrying platform 1, and the illumination light source 2 is fixedly provided on the liquid-carrying platform 1 and is located on one side of the light-transmitting window 11, and the light emitted by the illumination light source 2 is emitted from the light-transmitting window 11 into the liquid-carrying platform 1. Specifically, the light-transmitting window 11 is a light-transmitting glass, and the light-transmitting glass is inlaid on a side wall of the liquid-carrying platform 1. The light-transmitting glass can be made of a material whose refractive index is consistent with or similar to that of the detection medium. Such a setting can reduce the refraction of the light emitted by the illumination light source 2 in the process of passing through the light-transmitting glass and the detection medium to reach the sample 5, which is beneficial to improving the light propagation efficiency, and further enhancing the intensity of the fluorescence signal. In actual setting, an optical device such as an illumination objective lens can also be provided between the liquid-carrying platform 1 and the illumination light source 2 to converge the light of the illumination light source 2, so that the light of the illumination light source 2 is more irradiated on the sample 5, further enhancing the intensity of the fluorescence signal.

[0024] In this embodiment, the illumination light source 2 is an LED light source. LED light sources have the advantages of high luminous efficiency, long life, good stability, etc., and can meet the requirements of fluorescence detection for light sources. In actual use, LED light sources of corresponding wavelengths can be selected according to the fluorescence characteristics of sample 5 to better excite the fluorescence of sample 5. In addition, the size of the LED light source can be very small. Common LED patch lamp beads such as 3528 patch LEDs have a size of 3.5mm×2.8mm, 5050 patch LEDs have a size of 5.0mm×5.0mm, and 2835 patch LEDs have a size of 2.8mm×3.5mm. The small-sized illumination light source 2 is conducive to the small size and precision of the entire structure.

[0025] Furthermore, the sample fluorescence positioning system also includes a control unit (not shown in the figure), a displacement stage 4 and a driving unit (not shown in the figure). The displacement stage 4 is arranged on one side of the liquid carrier 1 and can be lifted and lowered to carry the sample 5; the driving unit is used to drive the displacement stage 4 to move; the control unit is electrically connected to the imaging element 3 and the driving unit. The fluorescent light emitted by the sample 5 is received by the imaging element 3, and the imaging element 3 sends the image signal to the control unit. The control unit determines the three-dimensional coordinates of the sample 5 according to the image signal, and controls the driving unit to drive the displacement stage 4 to move, and moves the sample 5 to the focal plane to complete the position calibration of the sample 5. It should be noted that the driving unit is a cylinder lifting structure, and can also be a screw lifting structure, etc., as long as the lifting function of the displacement stage 4 can be realized. The driving unit and the control unit are both existing technologies, and the specific structure and control principle of the control unit and the driving unit are not described in detail here. The control unit can receive the position information of the imaging element 3 in real time, so as to accurately determine the position of the sample 5, and the driving unit can accurately transfer the sample 5 to the focal plane, which provides convenience for subsequent experimental operations.

[0026] Specifically, the imaging element 3 may be an imaging camera or an imaging objective lens. The imaging objective lens or the imaging camera is disposed below the liquid stage 1 to receive the fluorescent light of the sample 5 to record the position of the sample 5. The imaging objective lens can directly capture the fluorescent image of the sample 5, which is convenient for subsequent analysis and processing; and the imaging objective lens can focus the fluorescent light to a specific position, which can improve the clarity and resolution of the imaging.

[0027] When in use, the sample 5 to be tested is first placed on the displacement stage 4, and then the illumination light source 2 is started to excite the fluorescence of the sample 5. The illumination light is irradiated onto the sample 5 through the light-transmitting window 11 and then received by the imaging element 3 after being reflected by the sample 5. The imaging element 3 transmits the fluorescence image to the control unit for processing and analysis. At the same time, the control unit can also calibrate the position of the sample 5 according to the position information of the imaging element 3, that is, the displacement stage 4 is driven to move by the driving unit to achieve accurate positioning of the sample 5.

[0028] More specifically, in this embodiment, the cross-section of the liquid carrier 1 is V-shaped, and connecting bosses 12 are arranged around the liquid carrier 1. Bolt holes are opened on the connecting bosses 12, and the liquid carrier 1 can be fixed to the light sheet fluorescence microscopy imaging system through the connecting bosses 12.

[0029] The sample fluorescence positioning system of the utility model realizes the fluorescence positioning of the sample 5 by using components such as the liquid carrier 1, the illumination light source 2, and the imaging element 3, thereby improving the accuracy and efficiency of the sample positioning. At the same time, by using optimization measures such as transparent glass and LED light source, the performance of the system is further improved. In addition, by using the control unit and the driving unit in coordination, the sample 5 is accurately positioned and moved, which provides convenience for subsequent experimental operations.

[0030] The above is a specific description of the preferred implementation of the utility model, but the invention of the utility model is not limited to the described embodiments. Technical personnel familiar with the field can also make various equivalent deformations or substitutions without violating the spirit of the utility model. These equivalent deformations or substitutions are all included in the scope defined by the claims of this application.

Claims

1. A sample fluorescence positioning system, characterized in that: It comprises a liquid carrier for carrying the detection medium and an imaging component, wherein the liquid carrier is provided with an illumination light source for exciting sample fluorescence, the imaging component is arranged on the liquid carrier, and the imaging component is used to receive the sample fluorescence light to record the position of the sample.

2. A sample fluorescence positioning system according to claim 1, characterized in that: The liquid carrier is provided with a light-transmitting window, and the illumination light source emits fluorescence to the sample from the light-transmitting window.

3. A sample fluorescence positioning system according to claim 2, characterized in that: The light-transmitting window is light-transmitting glass, and the refractive index of the light-transmitting glass is the same as the refractive index of the detection medium.

4. A sample fluorescence positioning system according to claim 1, characterized in that: The lighting source is an LED light source.

5. The sample fluorescence positioning system according to claim 1, characterized in that: The imaging element is an imaging camera or an imaging objective lens.

6. A sample fluorescence positioning system according to claim 1, characterized in that: It also includes a control unit, which is electrically connected to the imaging element and is used to receive position information of the imaging element to calibrate the position of the sample.

7. A sample fluorescence positioning system according to claim 6, characterized in that: It also includes a displacement stage and a driving unit. The displacement stage is arranged on one side of the liquid-carrying stage. The driving unit is electrically connected to the control unit and is used to drive the displacement stage to move.

8. The sample fluorescence positioning system according to claim 1, characterized in that: The liquid carrying platform is provided with a connecting boss, and the connecting boss is provided with a bolt hole.

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