Interventional needle absorption TIL tumor specimen taking device

Abstract

The utility model relates to a device for absorbing and taking a TIL tumor specimen through an interventional needle. The device comprises a primary screening bottle, a filter screen, a washing bottle and a collecting bottle, the primary screening bottle comprises a primary screening bottle cap and a primary screening bottle body, first hanging lugs are arranged on the two sides of the inner wall of the primary screening bottle body respectively, and a specimen placing hole is formed in the primary screening bottle cap; the filter screen comprises a screen cylinder and a base with filter holes, two vertically-through grooves are formed in the side wall of the screen cylinder, fixing columns are arranged on the two sides of the base respectively, and fixing blocks capable of moving up and down in the grooves in a matched mode are arranged on the upper portions of the fixing columns; first inserting buckles matched with the first hanging lugs are arranged on the outer sides of the fixing columns; the washing bottle comprises a washing turning cover and a washing bottle body, and second hanging lugs matched with the first inserting buckles are arranged on the two sides of the inner wall of the washing bottle body; the collecting bottle comprises a collecting bottle cap and a collecting bottle body which are connected through threads, and third hanging lugs matched with the first inserting buckles are arranged on the two sides of the inner wall of the collecting bottle.

Description

Technical Field

[0001] The utility model relates to a device for collecting specimens, in particular to a device for collecting TIL tumor specimens by absorbing an interventional needle. Background Art

[0002] At present, due to genetic factors and the influence of their own living habits, the number of people suffering from tumor diseases is gradually increasing. When cancer occurs, some lymphocytes in the body will migrate to the tumor and attack the tumor. However, the number of TILs in the patient's body is usually not enough to eliminate the tumor, and the tumor microenvironment will inhibit the function of TILs. Therefore, we can obtain tumor tissue from the patient's body through surgery or intervention and extract TILs, and then culture them in vitro to stimulate the proliferation of TILs. This in vitro stimulation method not only increases the number of TILs, but also activates the anti-tumor ability of TILs. These TILs are then injected back into the patient's body to kill tumor cells more effectively. According to literature, TIL therapy has the advantages of high specificity, wide safety window, wide target recognition, strong tumor tendency, and long duration. Compared with other therapies, TIL therapy is more suitable for various types of solid tumors. When obtaining TIL tumor specimens, some elderly and frail patients, those with poor cardiopulmonary function, severe hypertension, heart disease, heart failure, and mental illness, who cannot tolerate anesthesia, and those who are breastfeeding or pregnant and cannot tolerate major surgical operations, and the surgical department is unable to obtain specimens, we can collect TIL tumor specimens through intervention after evaluation.

[0003] Interventional needle aspiration biopsy is used to extract specimens of space-occupying diseases. It is safe, reliable, simple, easy to perform, has a high positive rate and few complications. Interventional needle aspiration biopsy refers to the use of a special puncture needle with a flexible catheter to enter the airway through the bronchoscope biopsy channel. The operating doctor determines the lesion site that needs to be punctured, punctures the needle tip into the lesion, connects the syringe, pulls out the syringe and generates negative pressure, and then the operator moves the puncture needle slightly forward and backward and rotates it, uses the bevel of the needle tip to absorb the specimen, and finally pulls out the puncture needle to collect the specimen.

[0004] The current specimen collection method is that a nurse holds the tail of the puncture needle to empty the syringe with negative pressure, and another nurse holds the needle tip of the puncture needle and fixes it in the dressing bowl to inject the specimen into solution A. Because the culture environment of TIL has high requirements for impurities, blood, and sterility, the nurse needs to use a sterile needle to pick up the required specimen and put it into the sterile medicine bowl for solution B to rinse, so as to rinse off excess necrotic tissue, impurities, blood, etc., in order to better and more efficient culture, and finally pick the rinsed specimen into the specimen bottle C for inspection. This method has the following problems in specimen processing:

[0005] (1) Currently, specimen processing requires a lot of manpower, at least two nurses are needed to operate, and it cannot be completed without sufficient manpower; (2) The required parts are numerous and fragmented, which can easily lead to omissions during preparation, increasing workload and time; (3) When the specimen is pushed out through a syringe, it is easy to splash, increasing the risk of infection; (4) When a needle is used to pick up specimens, the single amount is small and the success rate is low. After multiple picks, the tissue structure is easily damaged, affecting the culture of the specimen; (5) The dressing bowl that temporarily holds the specimen is in an open state, which increases the risk of specimen contamination. If there are patients with infectious diseases or carrying multidrug-resistant bacteria, there is a risk of secondary contamination in the handling of the specimens. Summary of the invention

[0006] The purpose of the utility model is to overcome the defects in the prior art and provide a device for obtaining TIL tumor specimens by absorbing with an interventional needle, which can be completed by a nurse alone and is simple, convenient, manpower-saving, time-saving and efficient.

[0007] The utility model is implemented as follows: an interventional needle absorption device for obtaining TIL tumor specimens, comprising a primary screening bottle, a filter screen, a washing bottle and a collection bottle; the primary screening bottle comprises a primary screening bottle cap and a primary screening bottle body, first hanging ears are respectively arranged on both sides of the inner wall of the primary screening bottle body, a specimen insertion hole is arranged on the primary screening bottle cap, elastic rubber plugs are arranged around the specimen insertion hole, and a hole is arranged in the middle of the elastic rubber plug;

[0008] The filter screen comprises a mesh cylinder and a base with filter holes, the side wall of the mesh cylinder is provided with two grooves which penetrate up and down, the two sides of the base are provided with fixing columns respectively, the upper part of the fixing columns is provided with a fixing block which can move up and down in the groove, the height of the mesh cylinder is less than or equal to the distance between the base and the fixing block, the bottom end of the mesh cylinder is in contact with the top surface of the base; the outer side of the fixing column is provided with a first buckle which matches with the first hanging ear;

[0009] The washing bottle comprises a washing flip cover and a washing bottle body, and the inner wall of the washing bottle body is provided with second hanging ears matched with the first buckle on both sides;

[0010] The collecting bottle comprises a collecting bottle cap and a collecting bottle body connected by threads, and third hanging ears cooperating with the first buckle are arranged on both sides of the inner wall of the collecting bottle.

[0011] The diameter of the upper opening of the mesh cylinder is smaller than the inner diameter of the primary screening bottle body and larger than the diameter of the lower opening of the mesh cylinder.

[0012] The outer part of the primary screening bottle cap is in a truncated cone shape as a whole.

[0013] The diameter of the upper end of the primary screening bottle cap is 2-3 cm, the diameter of the lower end matches the diameter of the bottle mouth of the primary screening bottle body, and the height of the primary screening bottle cap is 3-4 cm.

[0014] The primary screening bottle cap is connected to the primary screening bottle body through threads.

[0015] The primary screening bottle has a primary screening bottle body height of 5-7 cm and a diameter of 7-9 cm.

[0016] A circle of retaining walls is arranged on the top surface of the base near the edges thereof, and the lower end of the net cylinder is sleeved around the retaining walls.

[0017] The diameter of the upper opening of the mesh cylinder is 3-4 cm, the diameter of the lower opening of the mesh cylinder is 1.5-2.2 cm, and the overall height of the filter is 2.5-3.5 cm.

[0018] The beneficial effects of the utility model are as follows: by placing the filter screen in the primary screening bottle, the specimen is placed in the primary screening bottle for preliminary separation of tissue and tissue fluid, then the filter screen in the primary screening bottle together with the separated tissue is transferred to a washing bottle for washing, and finally the washed specimen is transferred to the collection bottle through the filter screen, and the specimen is finally transferred to the collection bottle for inspection by separating the mesh tube and the base of the filter screen. The whole process can be completed by a nurse alone, which is simple and convenient, saving manpower, time and efficiency; when the specimen is placed in the primary screening bottle, the primary screening bottle cap and the primary screening bottle body of the primary screening bottle are connected to each other, and the specimen is injected into the filter screen through the hole in the middle of the elastic rubber stopper, so that the primary screening bottle forms a closed type for collecting specimens to prevent splashing; the specimen is rinsed with a solution through the washing bottle, which is simple and efficient and reduces the risk of specimen contamination; the filter screen can completely separate the specimen from the tissue fluid, facilitates the extraction of the specimen, and increases the integrity of the specimen; the utility model can be sealed and discarded after use to avoid the occurrence of secondary contamination. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is a structural schematic diagram of a primary screening bottle of the utility model.

[0020] Figure 2 It is a structural schematic diagram of the utility model washing bottle.

[0021] Figure 3 It is a structural schematic diagram of the collecting bottle of the utility model.

[0022] Figure 4 It is a schematic diagram of the explosion structure of the filter screen of the utility model.

[0023] Figure 5 It is a structural schematic diagram of the filter screen of the utility model.

[0024] Figure 6 It is a structural schematic diagram of the utility model when the filter screen is placed in a primary screening bottle.

[0025] Among them: 1. primary screening bottle; 2. filter screen; 3. washing bottle; 4. collecting bottle; 5. primary screening bottle cap; 6. primary screening bottle body; 7. first hanging ear; 8. elastic rubber plug; 9. channel; 10. mesh tube; 11. filter hole; 12. base; 13. groove; 14. fixing column; 15. fixing block; 16. retaining wall; 17. first buckle; 18. washing flip cover; 19. washing bottle body; 20. cover buckle; 21. second hanging ear; 22. collecting bottle cap; 23. collecting bottle body; 24. third hanging ear. DETAILED DESCRIPTION

[0026] according to Figure 1 to Figure 6 The utility model is an interventional needle absorption device for obtaining TIL tumor specimens, comprising a primary screening bottle 1, a filter screen 2, a washing bottle 3 and a collecting bottle 4. The primary screening bottle 1 comprises a primary screening bottle cap 5 and a primary screening bottle body 6, and the primary screening bottle cap 5 is connected to the primary screening bottle body 6 by a thread. The outer part of the primary screening bottle cap 5 is truncated cone-shaped as a whole. The diameter of the upper end of the primary screening bottle cap 5 is 2~3cm, and the diameter of the lower end matches the diameter of the bottle mouth of the primary screening bottle body 6, and the height of the primary screening bottle cap is 3~4cm. The primary screening bottle body of the primary screening bottle has a height of 5~7cm and a diameter of 7~9cm.

[0027] First hanging ears 7 are respectively provided on both sides of the inner wall of the primary screening bottle body 6, and a specimen insertion hole is provided on the primary screening bottle cover 5. Elastic rubber plugs 8 are provided around the specimen insertion hole, and a channel 9 is provided in the middle of the elastic rubber plug 8.

[0028] The filter screen 2 includes a mesh tube 10 and a base 12 with a filter hole 11. The side wall of the mesh tube 10 is provided with two grooves 13 that pass through from top to bottom. Fixed columns 14 are provided on both sides of the base 12. The upper part of the fixed column 14 is provided with a fixed block 15 that can move up and down in the groove 13. The height of the mesh tube 10 is less than or equal to the distance between the base 12 and the fixed block 15. The upper diameter of the mesh tube 10 is less than the inner diameter of the primary screening bottle 6 and greater than the lower diameter of the mesh tube 10, forming a cylindrical shape with a large upper part and a small lower part. Preferably, the upper diameter of the mesh tube 10 is 3~4cm, preferably 3.8cm, and the lower diameter of the mesh tube 10 is 1.5~2.2cm. Preferably 2cm, the overall height of the filter screen 2 is 2.5~3.5cm, preferably 3cm. The net tube 10 is placed between the base 12 and the fixed block 15 by aligning the groove 13 with the fixed block 15 and moving downward, so that the bottom end of the net tube 10 contacts the top surface of the base 12; a circle of retaining walls 16 is provided near the edges of the top surface of the base 12, and the lower end of the net tube 10 is sleeved around the retaining walls 16. When the net tube 10 is located between the fixed block 15 and the base 12, it can rotate. When rotating, the fixed block 15 and the groove 13 are staggered, and the net tube 10 is restricted between the base 12 and the fixed block 15 in the up and down directions. The outer side of the fixed column 14 is provided with a first buckle 17 that matches the first hanging ear 7.

[0029] The washing bottle 3 includes a washing flip cover 18 and a washing bottle body 19. One side of the washing flip cover 18 and the washing bottle body 19 is hinged, and the other side of the washing flip cover 18 and the washing bottle body 19 are connected by a cover buckle 20. Second hanging ears 21 that cooperate with the first plug buckle 17 are provided on both sides of the inner wall of the washing bottle body 19.

[0030] The collecting bottle 4 comprises a collecting bottle cover 22 and a collecting bottle body 23 connected by threads, and third hanging ears 24 matching with the first buckle 17 are arranged on both sides of the inner wall of the collecting bottle 4 .

[0031] When collecting and processing needle aspiration specimens, the utility model first opens the primary screening bottle cap 5 of the primary screening bottle 1, places the filter screen 2 in the primary screening bottle 1, and inserts the first buckle 17 into the first hanging ear 7, then covers the primary screening bottle cap 5 and tightens it; sends the puncture needle with the specimen into the filter screen 2 through the channel 9 of the elastic rubber plug 8, at this time, the operator fixes the puncture needle with one hand and pushes the syringe with the other hand to push the specimen onto the base 12 of the filter screen 2, and the tissue fluid flows into the primary screening bottle body 6 along the filter hole 11, and the tissue block is retained on the base 12. After the specimen is pushed, the puncture needle is pulled out from the hole 9 of the elastic rubber stopper 8, the primary screening bottle cap 5 is opened, the top of the fixing column 14 of the filter screen 2 is pinched with one hand, the filter screen 2 containing the specimen is lifted out, the washing bottle body 19 of the washing bottle 3 is held with the other hand, and the washing flip cover 18 is opened through the cover buckle 20 with the thumb upward, and then the first plug buckle 17 of the filter screen 2 is connected and fixed with the second hanging ear 21 of the washing bottle body 19, the washing flip cover 18 is covered, and the washing bottle 3 is gently shaken so that the specimen can be fully rinsed in the solution of the washing bottle 3. After the rinsing is completed, the washing flip cover 18 is opened. Cover 18, and open the collecting bottle cover 22, connect and fix the first buckle 17 and the third ear 24, after fixing, rotate the net tube 10 left and right, align the groove 13 with the fixing block 15, lift and take out the net tube 10, and then connect the collecting bottle cover 22 with the collecting bottle body 23, gently shake the collecting bottle body 23 to make the specimen free into the specimen liquid in the collecting bottle body 23, then open the collecting bottle cover 22, take out the fixing column 14 and the base 12, place them in the primary screening bottle 1 or the washing bottle 3 and discard them, connect and fix the collecting bottle cover 22 with the collecting bottle body 23 and send them for inspection.

Claims

1. An interventional needle absorption device for obtaining TIL tumor specimens, characterized in that: It comprises a primary screening bottle, a filter screen, a washing bottle and a collecting bottle; the primary screening bottle comprises a primary screening bottle cap and a primary screening bottle body, first hanging ears are respectively arranged on both sides of the inner wall of the primary screening bottle body, a specimen insertion hole is arranged on the primary screening bottle cap, elastic rubber plugs are arranged around the specimen insertion hole, and a channel is arranged in the middle of the elastic rubber plug; The filter screen comprises a mesh cylinder and a base with filter holes, the side wall of the mesh cylinder is provided with two grooves which penetrate up and down, the two sides of the base are provided with fixing columns respectively, the upper part of the fixing columns is provided with a fixing block which can move up and down in the groove, the height of the mesh cylinder is less than or equal to the distance between the base and the fixing block, the bottom end of the mesh cylinder is in contact with the top surface of the base; the outer side of the fixing column is provided with a first buckle which matches with the first hanging ear; The washing bottle comprises a washing flip cover and a washing bottle body, and the inner wall of the washing bottle body is provided with second hanging ears matched with the first buckle on both sides; The collecting bottle comprises a collecting bottle cap and a collecting bottle body connected by threads, and third hanging ears cooperating with the first buckle are arranged on both sides of the inner wall of the collecting bottle.

2. The device for obtaining TIL tumor specimens by absorbing an interventional needle according to claim 1, characterized in that: The diameter of the upper opening of the mesh cylinder is smaller than the inner diameter of the primary screening bottle body and larger than the diameter of the lower opening of the mesh cylinder.

3. The device for obtaining TIL tumor specimens by absorbing an interventional needle according to claim 1, characterized in that: The outer part of the primary screening bottle cap is in a truncated cone shape as a whole.

4. The device for obtaining TIL tumor specimens by absorbing an interventional needle according to claim 3, characterized in that: The diameter of the upper end of the primary screening bottle cap is 2-3 cm, the diameter of the lower end matches the diameter of the bottle mouth of the primary screening bottle body, and the height of the primary screening bottle cap is 3-4 cm.

5. The device for obtaining TIL tumor specimens by absorbing an interventional needle according to claim 1 or 4, characterized in that: The primary screening bottle cap is connected to the primary screening bottle body through threads.

6. The device for obtaining TIL tumor specimens by absorbing an interventional needle according to claim 1 or 4, characterized in that: The primary screening bottle has a primary screening bottle body height of 5-7 cm and a diameter of 7-9 cm.

7. The device for obtaining TIL tumor specimens by absorbing an interventional needle according to claim 1, characterized in that: A circle of retaining walls is arranged on the top surface of the base near the edges thereof, and the lower end of the net cylinder is sleeved around the retaining walls.

8. The device for obtaining TIL tumor specimens by absorbing an interventional needle according to claim 1 or 2, characterized in that: The diameter of the upper opening of the mesh cylinder is 3-4 cm, the diameter of the lower opening of the mesh cylinder is 1.5-2.2 cm, and the overall height of the filter is 2.5-3.5 cm.

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