Purification device for gel chromatography

Abstract

The utility model relates to the technical field of gel chromatography, in particular to a purification device for gel chromatography, which comprises a vertical cylinder, the vertical cylinder is an uncovered cylinder with a notch on the side surface, and a graduated scale is mounted at the notch on the side surface of the vertical cylinder and is made of a transparent material; an interlayer is arranged outside the vertical cylinder, and a liquid inlet pipeline and a liquid outlet pipeline are arranged on the interlayer; the bottom of the vertical cylinder is detachably connected with the base, and a liquid discharge port is formed in the base; and a screen is fixed between the vertical cylinder and the base. According to the device, the temperature in the purification process can be controlled within a required range, meanwhile, the volume and the state of feed liquid in the vertical cylinder can be monitored in real time, the purification condition is controllable, the stability is high, the repeatability is high, and the research on the gel chromatography purification condition is facilitated; and the device is convenient to disassemble, clean and use, and is suitable for a gel chromatography purification process of batch operation.

Description

Technical Field

[0001] The utility model relates to the technical field of gel chromatography, in particular to a gel chromatography purification device. Background Art

[0002] Gel chromatography is a commonly used purification technology in the fields of biochemical engineering and biopharmaceuticals. Depending on the properties of the gel used in gel chromatography, the matching purification equipment and purification methods are also different. Among them, some gels swell at different ratios in solutions of different ionic strengths and pH values, resulting in large changes in gel volume due to different solutions used. Under normal pressure or pressurized conditions, they may deform significantly or even break, affecting the purification effect, such as Sephadex A-50 or C-50 ion exchange gel. Therefore, this part of the gel is not suitable for chromatography using a chromatography column.

[0003] In the prior art, the gel material is often subjected to gel chromatography by batch operation. The batch operation process is to place the gel in a purification device, add a batch of feed liquid or solution to be purified to the purification device for full contact and discharge, and then add the next batch of feed liquid or solution for full contact and discharge. The batch operation gel chromatography process adds feed liquid or solution in batches during the purification process, and is suitable for purification processes under conditions such as different gel expansion ratios and large feed liquid volumes.

[0004] However, the purification devices used in existing batch gel chromatography processes, especially those used in laboratory purification condition research, are often temporarily designed and assembled devices with a low degree of standardization. There are problems such as difficulty in controlling purification conditions such as temperature during gel chromatography, difficulty in observing the purification process, poor stability and repeatability, and difficulty in cleaning the devices. Utility Model Content

[0005] In view of the technical problems of low standardization of existing batch-type gel chromatography purification devices, difficult control of gel chromatography purification conditions, inconvenient observation of the experimental process, poor stability and repeatability, and difficult cleaning of the device, the utility model provides a gel chromatography purification device, which can control the temperature in the purification process within a required range, and can monitor the volume and state of the feed liquid in the vertical cylinder in real time. The purification conditions are controllable, the stability is strong, and the repeatability is high, which is conducive to the research on gel chromatography purification conditions; it is easy to disassemble and clean, easy to use, and suitable for batch-type gel chromatography purification processes.

[0006] The technical solution of the utility model is:

[0007] A gel chromatography purification device comprises a vertical cylinder, which is a coverless cylinder with a notch on the side, a scale made of transparent material is installed at the notch on the side of the vertical cylinder; an interlayer is arranged outside the vertical cylinder, and a liquid inlet pipe and a liquid outlet pipe are arranged on the interlayer; the bottom of the vertical cylinder is detachably connected to a base, and a liquid discharge port is arranged on the base; a screen is fixed between the vertical cylinder and the base.

[0008] Furthermore, at least two vertically upward screws are fixed on the base, and a base gasket groove is provided on the base; a vertical cylinder side edge is provided at the bottom of the vertical cylinder, and a screw groove and a vertical cylinder gasket groove corresponding to the position of the screw are provided on the vertical cylinder side edge; a screen frame is provided at the periphery of the screen, a first screen gasket groove is provided at a position corresponding to the vertical cylinder gasket groove on the top surface of the screen frame, and a second screen gasket groove is provided at a position corresponding to the base gasket groove on the bottom surface of the screen frame; the screw passes through the screw groove and is fastened by a nut; a first gasket is fixed between the vertical cylinder gasket groove and the first screen gasket groove, and a second gasket is fixed between the second screen gasket groove and the base gasket groove; gaskets are provided between the vertical cylinder and the screen, and between the screen and the base, which can improve the sealing performance of the purification device and prevent solution seepage during gel chromatography.

[0009] Furthermore, screw holes and side gasket grooves are arranged around the side notch of the vertical cylinder, and scale through holes and scale gasket grooves are arranged on the scale. The vertical cylinder and the scale are fastened together by screws, and a scale gasket is fixed between the side gasket groove and the scale gasket groove.

[0010] Furthermore, it also includes a scale fixing frame, on which are arranged fixing frame through holes corresponding to the positions of the scale through holes, screws pass through the fixing frame through holes, the scale through holes and the screw holes in sequence, and the scale fixing frame and the scale are fastened to the vertical tube, and the scale fixing frame can protect the scale.

[0011] Furthermore, a base groove is provided on the top surface of the base, which can leave space when the screen is slightly deformed due to the gravity of the gel, thereby avoiding damage to the screen or affecting the effect of chromatography purification.

[0012] Furthermore, the liquid discharge port is connected to a chuck-type quick-connect interface, which facilitates further detachable connection with a valve or pipeline with the same structure.

[0013] Furthermore, the vertical cylinder is made of stainless steel, the screen is made of stainless steel, and the aperture of the screen is 200-800 meshes.

[0014] Furthermore, a bracket is fixed at the bottom of the base to facilitate the placement of the purification device.

[0015] Furthermore, it also includes a vertical cylinder cover, which is detachably connected to the top of the vertical cylinder to prevent foreign matter from entering the vertical cylinder.

[0016] Furthermore, the vertical cylinder cover is composed of two identical semicircular cover plates, a center hole of the vertical cylinder cover is arranged in the center of the vertical cylinder cover, and a handle of the vertical cylinder cover is arranged on each semicircular cover plate. The center hole of the vertical cylinder can facilitate the insertion of the stirring device into the vertical cylinder, and the handle of the vertical cylinder cover can facilitate the taking of the vertical cylinder cover.

[0017] The beneficial effects of the utility model are:

[0018] 1. The gel chromatography purification device provided by the utility model has an interlayer arranged outside the vertical cylinder, and a liquid inlet pipe and a liquid outlet pipe are arranged on the interlayer, and a temperature control medium such as deionized water of a required temperature can be injected into the interlayer through the liquid inlet pipe and the liquid outlet pipe, so that the temperature in the purification process can be controlled within a required range, the purification conditions are controllable, the stability is strong, and the repeatability is high, which is conducive to the research on gel chromatography purification conditions.

[0019] 2. The utility model installs a transparent scale at the side notch of the vertical cylinder, through which the volume and state of the liquid in the vertical cylinder can be monitored in real time, which is helpful to grasp the progress and trend of the gel chromatography purification process and facilitate the standardized control of the purification process.

[0020] 3. The utility model connects the vertical cylinder and the base in a detachable manner, and fixes the screen between the vertical cylinder and the base. The purification device is easy to disassemble and clean, easy to use and operate, and is suitable for batch gel chromatography purification process. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] In order to more clearly illustrate the technical solutions in the embodiments of the utility model or the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, for ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0022] Figure 1 This is a front cross-sectional view of the purification device in Example 1.

[0023] Figure 2 This is a disassembled cross-sectional view of the purification device in Example 1.

[0024] Figure 3 It is a side cross-sectional view of the vertical tube in Example 1.

[0025] Figure 4 This is a top view of the vertical tube in Example 1.

[0026] Figure 5 This is the front view of the scale in Example 1.

[0027] Figure 6 It is a top view of the vertical cylinder cover in Example 2.

[0028] In the figure, 1-vertical cylinder, 11-side edge of the vertical cylinder, 12-nut, 13-scale, 14-scale fixing frame, 2-interlayer, 21-liquid inlet pipe, 22-liquid outlet pipe, 3-base, 31-liquid discharge port, 32-screw, 33-bracket, 4-screen, 41-screen frame, 42-first gasket, 43-second gasket, 51-semicircular cover plate, 52-vertical cylinder cover handle. DETAILED DESCRIPTION

[0029] In order to enable those skilled in the art to better understand the technical solutions in the present invention, the following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work should fall within the scope of protection of the present invention.

[0030] Example 1

[0031] like Figure 1-Figure 5 As shown, a gel chromatography purification device includes a vertical cylinder 1, which is a coverless cylinder with a notch on the side. The inner diameter of the vertical cylinder 1 is 80mm, the height is 250mm, and the material is stainless steel. Screw holes and side gasket grooves are arranged around the side notch of the vertical cylinder 1; it also includes a scale 13 and a scale fixing frame 14. The scale 13 is made of acrylic, the scale is 100-1000ml, and the interval between each two scales is 100m. l, the scale 13 is provided with a scale through hole and a scale gasket groove, the scale fixing frame 14 is provided with a fixing frame through hole corresponding to the position of the scale through hole, the screw passes through the fixing frame through hole, the scale through hole and the screw hole in sequence, and the scale fixing frame and the scale are fastened to the vertical tube, and a scale gasket is fixed between the side gasket groove and the scale gasket groove; the vertical tube 1 is provided with a vertical tube side edge 11 at the bottom, and four screw grooves are provided on the vertical tube side edge 11;

[0032] An interlayer 2 is arranged outside the vertical tube 1, and a liquid inlet pipe 21 and a liquid outlet pipe 22 are arranged on the interlayer 2;

[0033] The base 3 is also provided with a liquid discharge port 31, which is connected to a chuck-type quick-connect interface; a base groove is provided on the top surface of the base 3, and four screws 32 are fixed on the base 3, which are vertically upward and at positions corresponding to the screw grooves, and the screws 32 pass through the screw grooves and are fastened by nuts 12; a bracket 33 is fixed at the bottom of the base 3;

[0034] A screen 4 is fixed between the vertical cylinder 1 and the base 3. The screen 4 is made of stainless steel and has an aperture of 300 meshes. A screen frame 41 is provided on the periphery of the screen 4.

[0035] A base gasket groove is provided on the base 3; a vertical cylinder gasket groove is provided on the side edge 11 of the vertical cylinder; a first screen gasket groove is provided at a position corresponding to the top surface of the screen frame 41 and the vertical cylinder gasket groove, and a second screen gasket groove is provided at a position corresponding to the bottom surface of the screen frame 41 and the base gasket groove; a first gasket 42 is fixed between the vertical cylinder gasket groove and the first screen gasket groove, and a second gasket 43 is fixed between the second screen gasket groove and the base gasket groove.

[0036] Example 2

[0037] like Figure 1-Figure 6 As shown, based on Example 1, the gel chromatography purification device also includes a vertical cylinder cover, which is composed of two identical semicircular cover plates 51. A vertical cylinder cover center hole is set at the center of the vertical cylinder cover 5, and a vertical cylinder cover handle 52 is set on each semicircular cover plate 51.

[0038] Although the present invention is described in detail by referring to the accompanying drawings and in combination with the preferred embodiments, the present invention is not limited thereto. Without departing from the spirit and essence of the present invention, a person skilled in the art may make various equivalent modifications or substitutions to the embodiments of the present invention, and these modifications or substitutions shall be within the scope of the present invention. Any person skilled in the art who is familiar with the present technical field may easily think of changes or substitutions within the technical scope disclosed in the present invention, and these shall be within the scope of protection of the present invention.

Claims

1. A gel chromatography purification device, comprising a vertical column, characterized in that: The vertical cylinder is an uncovered cylinder with a notch on the side. A scale is installed at the notch on the side of the vertical cylinder, and the scale is made of transparent material; an interlayer is arranged outside the vertical cylinder, and a liquid inlet pipe and a liquid outlet pipe are arranged on the interlayer; the bottom of the vertical cylinder is detachably connected to the base, and a liquid discharge port is arranged on the base; a screen is fixed between the vertical cylinder and the base.

2. The purification device according to claim 1, characterized in that At least two vertically upward screws are fixed on the base, and a base gasket groove is provided on the base; a vertical cylinder side edge is provided at the bottom of the vertical cylinder, and a screw groove and a vertical cylinder gasket groove corresponding to the position of the screw are provided on the vertical cylinder side edge; a screen frame is provided at the periphery of the screen, a first screen gasket groove is provided at a position corresponding to the vertical cylinder gasket groove on the top surface of the screen frame, and a second screen gasket groove is provided at a position corresponding to the base gasket groove on the bottom surface of the screen frame; the screw passes through the screw groove and is fastened by a nut; a first gasket is fixed between the vertical cylinder gasket groove and the first screen gasket groove, and a second gasket is fixed between the second screen gasket groove and the base gasket groove.

3. The purification device according to claim 1, characterized in that Screw holes and side gasket grooves are arranged around the side notch of the vertical tube, and scale through holes and scale gasket grooves are arranged on the scale. The vertical tube and the scale are fastened and connected by screws, and a scale gasket is fixed between the side gasket groove and the scale gasket groove.

4. The purification device according to claim 3, characterized in that: The scale fixing frame is provided with fixing frame through holes corresponding to the positions of the scale through holes. Screws pass through the fixing frame through holes, the scale through holes and the screw holes in sequence to fasten the scale fixing frame and the scale to the vertical tube.

5. The purification device according to claim 1, characterized in that: The top surface of the base is provided with a base groove.

6. The purification device according to claim 1, characterized in that The liquid discharge port is connected with a chuck-type quick-connect interface.

7. The purification device according to claim 1, characterized in that The vertical cylinder is made of stainless steel, the screen is made of stainless steel, and the aperture of the screen is 200-800 mesh.

8. The purification device according to claim 1, characterized in that A bracket is fixed to the bottom of the base.

9. The purification device according to claim 1, characterized in that: It also includes a vertical cylinder cover, which is detachably connected to the top of the vertical cylinder.

10. The purification device according to claim 9, characterized in that The vertical cylinder cover is composed of two identical semicircular cover plates, a central hole of the vertical cylinder cover is arranged at the center of the vertical cylinder cover, and each semicircular cover plate is provided with a handle of the vertical cylinder cover.

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