Microfluidic tool box

Abstract

The utility model discloses a microfluidics tool box, including fixture body and chip, fixture body includes body and gland subassembly, body has a plurality of through-hole and is provided with the chip slot of its top, each through-hole one end is located in the slot bottom of chip slot, the other end passes through the body peripheral wall, the chip is provided in the chip slot, and the gland subassembly is provided with the gland subassembly. A liquid outlet hole connected with the through hole and a plurality of liquid inlet holes are formed in one face of the chip, a liquid flow channel communicating the liquid outlet hole with the liquid inlet holes is further formed in the chip, the gland assembly covers the top of the body, the chip is tightly pressed in the chip groove and is connected with the body through a bolt, and a temperature controller is further arranged in the body and located under the chip groove. By simplifying the structure of the gland assembly, the gland assembly can be quickly disassembled and assembled so as to quickly replace the chip, meanwhile, the temperature controller is arranged in the body, temperature adjustment can be carried out during working, other special temperature control platforms do not need to be adopted, and the use convenience of the product is greatly improved.

Description

Technical Field

[0001] The utility model relates to the technical field of microfluidics, and in particular to a microfluidics tool box. Background Art

[0002] A microfluidic tool box usually includes a chip fixture and a microfluidic chip. The chip fixture is used to fix and connect the chip to ensure the stability and reliability of the microfluidic chip, while the chip is a key component for microfluidic experiments. These tools can help researchers conduct microfluidic experiments.

[0003] The chip fixtures of the microfluidic tool boxes currently on the market are not only complex in structure and difficult to replace chips, but also lack temperature control functions and require a dedicated heating platform, which is inconvenient to use. Utility Model Content

[0004] In view of the deficiencies of the prior art, the utility model provides a microfluidic tool box, comprising:

[0005] The clamp body comprises a body and a pressure cover assembly, wherein the body has a chip slot and a plurality of conducting holes, wherein the chip slot is arranged at the top of the body, and one end of each conducting hole is located at the bottom of the chip slot, and the other end penetrates through the peripheral wall of the body; and

[0006] The chip is arranged in the chip slot, and one side of the chip has a liquid outlet and a plurality of liquid inlet holes. The chip also has a liquid flow channel inside, and the liquid flow channel connects the liquid outlet and the liquid inlet holes to each other, and the liquid outlet and the plurality of liquid inlet holes are connected to a conducting hole respectively;

[0007] The pressure cover assembly is installed on the top of the body and presses the chip into the chip slot. It is connected to the body with bolts.

[0008] The main body also has a temperature controller, which is arranged inside the main body and directly below the chip slot.

[0009] According to one embodiment of the utility model, the main body includes a main body and a bottom cover, the chip slot and the conductive hole are both arranged on the main body, the main body also has a temperature control groove, the temperature control groove is opened at the bottom of the main body, the temperature controller is arranged in the temperature control groove, and the bottom cover is arranged at the bottom of the main body and closes the temperature control groove.

[0010] According to one embodiment of the utility model, the top of the main body also has a pressing groove, the chip groove is arranged in the pressing groove, the pressure cover assembly includes a fixed frame and a pressure plate, the pressure plate is arranged in the pressing groove and covers the chip, an observation port is opened at the center of the fixed frame, the observation port is connected to the pressing groove, the fixed frame cover is arranged on the top of the main body, and the surrounding wall of the observation port covers the surface of the pressure plate, and the pressure plate and the chip are both made of transparent materials.

[0011] According to one embodiment of the utility model, the top of the main body also has a plurality of positioning holes, the positioning holes are circumferentially arranged around the pressing groove, and the fixing frame is provided with a plurality of positioning pins corresponding to the positioning holes, and the positioning pins are respectively inserted into the positioning holes.

[0012] According to one embodiment of the utility model, the chip includes a chip body and a cover plate, the liquid outlet and the liquid inlet are both arranged on the chip body, the liquid flow channel includes a mixing flow channel and a plurality of liquid inlet flow channels engraved on the surface of the chip body, one end of the liquid inlet flow channel is respectively connected to a liquid inlet hole, one end of the mixing flow channel is connected to the liquid outlet, the other ends of the plurality of liquid inlet flow channels and the other end of the mixing flow channel are connected to each other, the cover plate is arranged on one side of the chip body, and closes the liquid flow channel.

[0013] According to one embodiment of the utility model, the mixing channel has a plurality of mixing zones, which are arranged in sequence along the length direction of the mixing channel, and each mixing zone includes a recessed area and a liquid blocking protrusion which are arranged on two side walls of the mixing channel, wherein one end of the liquid blocking protrusion extends into the recessed area and forms a C-shaped channel with the recessed area, and the C-shaped channels of the plurality of mixing zones are connected end to end in sequence, and one end of the first C-shaped channel is connected to one end of the two liquid inlet channels, and one end of the last C-shaped channel is connected to the liquid outlet.

[0014] According to one embodiment of the utility model, the inner wall of the recessed area has a first liquid stopper head, and one end of the liquid stopper protrusion has a second liquid stopper head. The first liquid stopper head and the second liquid stopper head are respectively located on the two inner walls of the C-shaped flow channel and are arranged opposite to each other. The cross-sectional shapes of the first liquid stopper head and the second liquid stopper head are both right-angled triangles, and the right-angle ends of the two are respectively facing the liquid inflow direction and the liquid outflow direction in the C-shaped flow channel.

[0015] According to one embodiment of the utility model, each liquid blocking protrusion also has two V-shaped mixing grooves, which are respectively arranged on the two side walls of the liquid blocking protrusion and located at one end where the liquid blocking protrusion is connected to the side wall of the mixing flow channel. One of the side walls of each V-shaped mixing groove is flush with the side wall of the mixing flow channel, and the other side wall is inclined toward the direction of the C-shaped flow channel.

[0016] According to an embodiment of the present invention, along the length direction of the mixing channel, a plurality of mixing zones are alternately arranged on both sides of the central axis aa, and the C-shaped channels thereof are all oriented toward the central axis aa.

[0017] According to one embodiment of the utility model, the chip slot also has a positioning column, which is arranged at one end of the bottom of the chip slot and extends vertically upward. The chip is provided with a positioning through hole corresponding to the positioning column, and the positioning through hole runs through the chip body and the cover plate.

[0018] The beneficial effects of the utility model are as follows: by simplifying the structure of the pressure cover assembly, the pressure cover assembly can be quickly disassembled and assembled, thereby realizing the rapid replacement of the chip. At the same time, by arranging a temperature controller in the body, the temperature can be adjusted during operation without the need to use other dedicated temperature control platforms, thereby greatly improving the convenience of product use. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] The drawings described herein are used to provide a further understanding of the present application and constitute a part of the present application. The illustrative embodiments of the present application and their descriptions are used to explain the present application and do not constitute an improper limitation on the present application. In the drawings:

[0020] Figure 1 A schematic diagram of the structure of the microfluidic tool box in the embodiment;

[0021] Figure 2 This is one of the exploded structural schematic diagrams of the microfluidic tool box in the embodiment;

[0022] Figure 3 The second schematic diagram of the exploded structure of the microfluidic tool box in the embodiment;

[0023] Figure 4 Schematic diagram of the structure of the main body of the chip in the embodiment;

[0024] Figure 5 It is a schematic diagram of the structure of the chip body in the embodiment;

[0025] Figure 6 for Figure 5 Enlarged view of part A in the middle. DETAILED DESCRIPTION

[0026] The following will disclose multiple embodiments of the present invention with drawings. For the purpose of clear description, many practical details will be described together in the following description. However, it should be understood that these practical details should not be used to limit the present invention. In other words, in some embodiments of the present invention, these practical details are not necessary. In addition, in order to simplify the drawings, some conventional structures and components will be depicted in a simple schematic manner in the drawings.

[0027] In addition, in the present utility model, the descriptions of "first", "second", etc. are only used for descriptive purposes, and do not specifically refer to the order or sequence, nor are they used to limit the present utility model. They are only used to distinguish components or operations described with the same technical terms, and cannot be understood as indicating or implying their relative importance or implicitly indicating the number of technical features indicated. Therefore, the features defined as "first" and "second" may explicitly or implicitly include at least one of the features. In addition, the technical solutions between the various embodiments can be combined with each other, but they must be based on the ability of ordinary technicians in this field to implement them. When the combination of technical solutions is contradictory or cannot be implemented, it should be deemed that such a combination of technical solutions does not exist and is not within the scope of protection required by the present utility model.

[0028] See also Figure 1-Figure 5 , Figure 1 is a schematic diagram of the structure of the microfluidic tool box in the embodiment, Figure 2 This is one of the exploded structural diagrams of the microfluidic tool box in the embodiment, Figure 3 This is the second schematic diagram of the exploded structure of the microfluidic tool box in the embodiment. Figure 4 is a schematic structural diagram of the main body of the chip in the embodiment, Figure 5 Schematic diagram of the structure of the chip body in the embodiment. A microfluidic tool box in this example includes a clamp body 1 and a chip 2. The clamp body 1 includes a body 11 and a gland assembly 12. The body 11 has a chip slot 1111 and a plurality of conduction holes 1112. The chip slot 1111 is arranged at the top of the body 1. One end of each conduction hole 1112 is located at the bottom of the chip slot 1111, and the other end penetrates the peripheral wall of the body 11. The chip 2 is arranged in the chip slot 1111, and one side thereof has a liquid outlet 211 and a plurality of liquid inlet holes 212. The chip 2 also has a liquid flow channel 213 inside. The liquid flow channel 213 connects the liquid outlet 211 and the liquid inlet 212 to each other, and the liquid outlet 211 and the plurality of liquid inlet holes 212 are connected to a conduction hole 1112 respectively. In specific implementation, liquid can be injected into the liquid flow channel 213 in the chip 2 through the conduction hole 1112 or the mixed liquid in the liquid flow channel 213 can be discharged and collected.

[0029] The pressure cover assembly 12 is placed on the top of the body 11 and presses the chip 2 into the chip slot 1111. The pressure cover assembly 12 is connected to the body 11 by bolts. The body 11 also has a temperature controller (not shown). The temperature controller (not shown) is placed inside the body and directly below the chip slot 1111. In this example, the temperature controller is a Peltier temperature controller.

[0030] In this way, when in use, it is only necessary to remove the bolts of the pressure cover assembly 12, then the pressure cover assembly 12 can be opened and the chip 2 in the chip slot 1111 can be replaced, which greatly improves the convenience of use. At the same time, since a temperature controller is provided in the main body 11, the temperature of the fluid in the chip 2 can be directly heated, kept warm, etc. by the temperature controller during operation, without the need to use other dedicated temperature control platforms, further improving the convenience of use.

[0031] Preferably, one end of the conducting hole 1112 located on the side wall of the main body 11 has an internal thread. When in use, a threaded pipe connector or a Luer quick-release connector can be used to quickly connect to the conducting hole 1112.

[0032] Furthermore, the body 11 includes a main body 111 and a bottom cover 112. The chip slot 1111 and the through hole 1112 are both provided on the main body 111. The main body 111 also has a temperature control slot 1113. The temperature control slot 1113 is provided at the bottom of the main body 111. The thermostat (not shown) is provided in the temperature control slot 1113. The bottom cover 112 is provided at the bottom of the main body 111 and closes the temperature control slot 1113. The bottom cover 112 is also connected to the main body 111 by bolts. Similarly, when the thermostat needs to be maintained or replaced, it is only necessary to remove the bolts of the bottom cover 112, which further improves the convenience of product use.

[0033] Review Figure 1-Figure 4 Furthermore, the top of the main body 111 also has a pressing groove 1114, the chip groove 1111 is arranged in the pressing groove 1114, the pressure cover assembly 12 includes a fixed frame 121 and a pressing plate 122, the pressing plate 122 is arranged in the pressing groove 1114 and covers the chip 2, an observation port 1211 is opened at the center of the fixed frame 121, the observation port 1211 is connected to the pressing groove 1114, the fixed frame 121 is covered at the top of the main body 111, and the peripheral wall of the observation port 1211 covers the surface of the pressing plate 122, and the pressing plate 122 and the chip 2 are both made of transparent materials. In this example, the pressing plate 122 and the chip 2 are made of polymers such as cycloolefins or glass. During use, after the chip 2 is loaded into the chip slot 1111, the pressure plate 122 is installed in the pressure slot 1114. At this time, the fixing frame 121 is installed on the top 111 of the main body and locked with bolts. At this time, the fixing frame 121 presses the pressure plate 122 in the pressure slot 1114, and then the pressure plate 122 presses the chip 2 in the chip slot 1111, which also ensures the stability of the chip. At the same time, it can be understood that since the chip 2 and the pressure plate 122 are both made of transparent materials, the user can observe the mixing of the liquid in the chip 2 through the observation port 1211, and make timely adjustments to the injection to further improve the convenience of product use.

[0034] Review Figure 1-Figure 4Preferably, the top of the main body 111 also has a plurality of positioning holes 1115, which are arranged circumferentially around the pressing groove 1114, and the fixing frame 121 is provided with a plurality of positioning pins 1212 corresponding to the positioning holes 1115, and the positioning pins 1212 are respectively inserted into the positioning holes 1115. That is, during installation, the positioning pins 1212 of the fixing frame 121 can be aligned and inserted into the positioning holes 1115, so that the fixing frame 121 is quickly aligned with the threaded holes of the main body 111, and then bolts can be used for quick fixing, so that the installation frame 121 can be quickly installed.

[0035] participate Figure 6 , review Figure 5 , Figure 6 for Figure 5 A magnified view of the middle A part. Furthermore, the chip 2 includes a chip body 21 and a cover plate 22, the liquid outlet hole 211 and the liquid inlet hole 212 are both provided on the chip body 21, the liquid flow channel 213 includes a mixing flow channel 2131 and a plurality of liquid inlet flow channels 2132 engraved on the surface of the chip body 21, one end of the liquid inlet flow channel 2132 is respectively connected to one of the liquid inlet holes 212, one end of the mixing flow channel 2131 is connected to the liquid outlet hole 211, the other ends of the plurality of liquid inlet flow channels 2132 and the other end of the mixing flow channel 2131 are connected to each other, and the cover plate 22 is provided on one side of the chip body 21 to close the liquid flow channel 213. During operation, the reagents enter the liquid inlet channel 2132 through two liquid inlet holes 212 respectively after passing through two of the conducting holes 1112, and flow together to the mixing channel 2131 for mixing. The mixed sample flows out from the liquid outlet hole 211 to the other conducting hole 1112, and the mixed sample can be collected through the conducting hole 1112 at this time.

[0036] Specifically, the mixing channel 2131 has a plurality of mixing zones 21311, and the plurality of mixing zones 21311 are arranged in sequence along the length direction of the mixing channel 2131, and each mixing zone 21311 includes a recessed zone 21312 and a liquid blocking protrusion 21313 respectively arranged on the two side walls of the mixing channel 2131, wherein one end of the liquid blocking protrusion 21313 extends into the recessed zone 21312, and forms a C-shaped channel 213111 between the recessed zone 21312, and the C-shaped channels 213111 of the plurality of mixing zones 21311 are connected end to end in sequence, and one end of the first C-shaped channel 213111 is connected to one end of the two liquid inlet channels 2132, and one end of the last C-shaped channel 213111 is connected to the liquid outlet hole 2111.

[0037] Among them, the inner wall of the recessed area 21312 has a first liquid blocking head 213121, and one end of the liquid blocking protrusion 21313 has a second liquid blocking head 213131. The first liquid blocking head 213121 and the second liquid blocking head 213131 are respectively located on the two inner walls of the C-shaped flow channel 213111 and are arranged opposite to each other. The cross-sectional shapes of the first liquid blocking head 213121 and the second liquid blocking head 213131 are both right-angled triangles, and the right-angle ends of the two are respectively facing the liquid inflow direction and liquid outflow direction in the C-shaped flow channel 213111.

[0038] Preferably, each liquid blocking protrusion 21313 also has two V-shaped mixing grooves 213132, and the two V-shaped mixing grooves 213132 are respectively arranged on the two side walls of the liquid blocking protrusion 21313, and are located at one end where the liquid blocking protrusion 21313 is connected to the side wall of the mixing channel 2131, and one of the side walls of each V-shaped mixing groove 213132 is flush with the side wall of the mixing channel 2131, and the other side wall is inclined toward the direction of the C-shaped channel 213111.

[0039] During operation, when the liquids of the two liquid inlet channels 2132 enter the mixing channel 2131 to form a mixed liquid, the mixed liquid sequentially enters a plurality of mixing areas 21311 to be fully mixed. Specifically, when entering each mixing area 21311, a portion of the mixed fluid directly flows into the C-shaped channel 213111, and the other portion enters the V-shaped mixing groove 213132 to form a reflux and enters the C-shaped channel 213111 after being fully mixed. In the C-shaped channel 213111, the mixed liquid impacts the first liquid blocking head 213121 and the second liquid blocking head 213131, respectively. The mixed liquid is offset in the flow direction toward the liquid blocking protrusion 21313. Conversely, the mixed liquid that impacts the second liquid blocking head 213131 is offset in the flow direction toward the inner wall of the recessed area 21312. That is, the first liquid blocking head 213121 and the second liquid blocking head 231313 respectively cause the mixed liquid to be offset in opposite directions, causing cross-staggered mixing, thereby improving the mixing effect. At the same time, since there is no obvious collision structure, the resistance to liquid circulation can be effectively reduced to prevent the pipeline from being broken and leaking when the liquid flux is too large, thereby achieving high-throughput and high-flow rate liquid mixing.

[0040] When the liquid is about to flow out of the C-shaped flow channel 213111, similarly, part of it enters the next mixing zone 213111 through the C-shaped flow channel 213111, and the other part enters the V-shaped mixing groove 213132 located at the outlet of the C-shaped flow channel 213111 to produce reflux mixing before flowing out and entering the next mixing zone 213111.

[0041] Furthermore, along the length direction of the mixing channel 2131, a plurality of mixing zones 21311 are alternately arranged on both sides of the central axis aa, and the C-shaped channels 213111 thereof face the central axis aa. In this way, when the mixed liquid enters the adjacent mixing zone, it can directly follow the outlet direction of the C-shaped channel 213111 to enter the inlet of the next C-shaped channel 213111, thereby avoiding direct collision between the mixed liquid and the inner wall of the mixing channel 2131 to increase resistance, further improving product throughput, and not only that, the V-shaped mixing groove 213132 at the outlet of the previous C-shaped channel 213111 and the V-shaped mixing groove 213132 at the inlet of the next C-shaped channel 213111 again cause the mixed liquid to deviate in opposite directions and cross-mix, further improving the mixing effect.

[0042] Review Figure 1-Figure 4 Preferably, the chip slot 1111 further has a positioning post 11111, which is disposed at the bottom of the chip slot 1111 and extends vertically upward, and the chip 2 is provided with a positioning through hole 214 corresponding to the positioning post 11111, and the positioning through hole 214 runs through the chip body 21 and the cover plate 22. When the chip 2 needs to be installed in the chip slot 1111, the positioning through hole 214 can be aligned with the positioning post 11111, so as to realize fast and accurate installation of the chip 2 and prevent the chip 2 from being offset.

[0043] Preferably, the bottom of the chip slot 1111 also has a plurality of placement slots 11112, wherein one end of the plurality of conductive holes 1112 are respectively located at the bottom of the plurality of placement slots 11112. In specific implementation, a sealing gasket 11113 can be placed in the placement slot 11112, so that its two ends respectively abut against the surrounding wall of one end of the conductive hole 1112 and the surrounding wall of one end of the liquid inlet hole 212 or the liquid outlet hole 211 to prevent the chip 2 from leaking during operation.

[0044] In summary, in this example, the product structure is effectively simplified by connecting the cover assembly with the main body through bolts, and the quick disassembly and assembly of the cover assembly is realized, thereby realizing the quick replacement of the chip. At the same time, by arranging a temperature controller in the main body, the temperature can be adjusted during operation without the need to use other dedicated temperature control platforms, thereby greatly improving the convenience of product use.

[0045] The above is only an embodiment of the present invention and is not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent substitution, improvement, etc. made within the spirit and principle of the present invention shall be included in the scope of the claims of the present invention.

Claims

1. A microfluidic tool box, characterized in that: include: A clamp body (1) comprises a body (11) and a pressure cover assembly (12), wherein the body (11) has a chip slot (1111) and a plurality of conducting holes (1112), wherein the chip slot (1111) is arranged at the top of the body (11), and each of the conducting holes (1112) has one end located at the bottom of the chip slot (1111) and the other end penetrating through the peripheral wall of the body (11); and A chip (2) is arranged in the chip slot (1111), and has a liquid outlet hole (211) and a plurality of liquid inlet holes (212) on one side. The chip (2) also has a liquid flow channel (213) inside. The liquid flow channel (213) respectively connects the liquid outlet hole (211) and the liquid inlet hole (212) to each other. The liquid outlet hole (211) and the plurality of liquid inlet holes (212) are respectively connected to one of the conducting holes (1112); The pressure cover assembly (12) is covered on the top of the body (11) and presses the chip (2) into the chip slot (1111), and is connected to the body (11) by bolts; The main body (11) also has a temperature controller, which is arranged inside the main body and directly below the chip slot (1111).

2. The microfluidic tool box according to claim 1, characterized in that: The main body (11) comprises a main body (111) and a bottom cover (112); the chip slot (1111) and the conducting hole (1112) are both arranged on the main body (111); the main body (111) also has a temperature control groove (1113); the temperature control groove (1113) is opened at the bottom end of the main body (111); the temperature controller is arranged in the temperature control groove (1113); the bottom cover (112) is arranged on the bottom of the main body (111) and closes the temperature control groove (1113).

3. The microfluidic tool box according to claim 2, characterized in that: The main body (111) also has a pressing groove (1114) at the top, and the chip groove (1111) is arranged in the pressing groove (1114). The pressure cover assembly (12) includes a fixing frame (121) and a pressing plate (122). The pressing plate (122) is arranged in the pressing groove (1114) and covers the chip (2). An observation port (1211) is opened at the center of the fixing frame (121), and the observation port (1211) is connected to the pressing groove (1114). The fixing frame (121) is covered on the top of the main body (111), and the peripheral wall of the observation port (1211) covers the surface of the pressing plate (122). The pressing plate (122) and the chip (2) are both made of transparent materials.

4. The microfluidic tool box according to claim 3, characterized in that: The main body (111) also has a plurality of positioning holes (1115) on the top, the positioning holes (1115) are circumferentially arranged around the pressing groove (1114), and the fixing frame (121) is provided with a plurality of positioning pins (1212) corresponding to the positioning holes (1115), and the positioning pins (1212) are respectively inserted into the positioning holes (1115).

5. The microfluidic tool kit according to claim 1, characterized in that: The chip (2) comprises a chip body (21) and a cover plate (22); the liquid outlet hole (211) and the liquid inlet hole (212) are both arranged on the chip body (21); the liquid flow channel (213) comprises a mixing flow channel (2131) and a plurality of liquid inlet flow channels (2132) engraved on the surface of the chip body (21); one end of each of the liquid inlet flow channels (2132) is connected to one of the liquid inlet holes (212); one end of the mixing flow channel (2131) is connected to the liquid outlet hole (211); the other ends of the plurality of liquid inlet flow channels (2132) and the other end of the mixing flow channel (2131) are connected to each other; the cover plate (22) is arranged on one side of the chip body (21) to close the liquid flow channel (213).

6. The microfluidic tool box according to claim 5, characterized in that: The mixing channel (2131) has a plurality of mixing areas (21311), and the plurality of mixing areas (21311) are arranged in sequence along the length direction of the mixing channel (2131), and each of the mixing areas (21311) comprises a recessed area (21312) and a liquid blocking protrusion (21313) respectively arranged on two side walls of the mixing channel (2131), wherein one end of the liquid blocking protrusion (21313) extends into the recessed area (21312) and forms a C-shaped channel (213111) between the recessed area (21312), and the C-shaped channels (213111) of the plurality of mixing areas (21311) are connected end to end in sequence, and one end of the first C-shaped channel (213111) is connected to one end of the two liquid inlet channels (2132), and one end of the last C-shaped channel (213111) is connected to the liquid outlet hole (2111).

7. The microfluidic tool box according to claim 6, characterized in that: The inner wall of the recessed area (21312) has a first liquid stopper (213121), and one end of the liquid stopper protrusion (21313) has a second liquid stopper (213131). The first liquid stopper (213121) and the second liquid stopper (213131) are respectively located on the two inner walls of the C-shaped flow channel (213111) and are arranged opposite to each other. The cross-sectional shapes of the first liquid stopper (213121) and the second liquid stopper (213131) are both right-angled triangles, and the right-angle ends of the two are respectively facing the liquid inflow direction and the liquid outflow direction in the C-shaped flow channel (213111).

8. The microfluidic tool box according to claim 6, characterized in that: Each of the liquid blocking protrusions (21313) also has two V-shaped mixing grooves (213132), which are respectively arranged on the two side walls of the liquid blocking protrusion (21313), and are located at one end where the liquid blocking protrusion (21313) is connected to the side wall of the mixing channel (2131), and one of the side walls of each of the V-shaped mixing grooves (213132) is flush with the side wall of the mixing channel (2131), and the other side wall is inclined toward the C-shaped channel (213111).

9. The microfluidic tool box according to claim 6, characterized in that: Along the length direction of the mixing channel (2131), the plurality of mixing zones (21311) are alternately arranged on both sides of the central axis aa, and their C-shaped channels (213111) are all facing the central axis aa.

10. The microfluidic tool box according to claim 5, characterized in that: The chip slot (1111) also has a positioning column (11111), the positioning column (11111) is arranged at one end of the bottom of the chip slot (1111) and extends vertically upwards, the chip (2) is provided with a positioning through hole (214) corresponding to the positioning column (11111), and the positioning through hole (214) is arranged through the chip body (21) and the cover plate (22).

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