Cell detector based on electrical impedance

Abstract

The utility model provides a cell detector based on electrical impedance, which comprises a conveying device, a filling device, a detecting device and a sorting device, the conveying device comprises a conveying belt and a screw rod sliding table, the conveying belt is used for conveying test tubes to a specified position, and the screw rod sliding table is used for controlling the lifting of the test tubes; the filling device comprises a filling main machine, a filling gun and a hydraulic telescopic cylinder, the filling gun and the hydraulic telescopic cylinder are both installed in the filling main machine, and the telescopic length of the filling gun is controlled through the hydraulic telescopic cylinder; the detection device comprises an upper computer and an electrical impedance detection device, and the electrical impedance detection device is connected with the upper computer; the sorting device comprises a rotating assembly and a grabbing assembly, the rotating assembly comprises a rotating disc, a rotating motor and a plurality of mechanical claws, and the grabbing assembly comprises an electric claw, a connecting block, an air cylinder and a sliding rail. According to the utility model, the morphology and the structure of cells can be observed, the cell detection efficiency and the analysis accuracy are improved, the operation is simple, and the cost is lower.

Description

Technical Field

[0001] The utility model relates to the technical field of cell detection, in particular to a cell detector based on electrical impedance. Background Art

[0002] With the rapid development of medical equipment automation technology, the automation level of medical laboratories is also constantly improving. Traditional manual operations and semi-automatic equipment have been gradually replaced by automated equipment, which has enabled laboratory staff to shift from physical labor to more precise and efficient mental labor. Modern medical automated analysis equipment has gradually entered people's field of vision, and equipment such as fully automatic cell analyzers are also becoming increasingly popular. However, the existing automatic detection technology is mainly based on flow cytometry technology. The existing automated detection technology has the following main disadvantages:

[0003] First, although flow cytometry can detect and analyze a large number of single cells and measure multiple parameters of single cells, it cannot observe the morphology and structure of cells;

[0004] Second, the imaging speed of fluorescence microscopy technology is slow, and it has certain requirements on the selection of markers and the stability of markers, and it cannot achieve high-throughput detection of a large number of samples;

[0005] Third, automated image analysis technology has high requirements for image quality and stability of analysis algorithms, and there are problems of errors and missed detections. It also has high requirements for hardware equipment and software algorithms, resulting in high costs.

[0006] Fourth, the overall efficiency of existing technologies for cell detection is low. Utility Model Content

[0007] In order to solve the above technical problems, the utility model provides a cell detector based on electrical impedance, which can observe the morphology and structure of cells, improve the efficiency of cell detection and the accuracy of analysis, and is simple to operate and low in cost.

[0008] The technical solution adopted by the utility model is as follows:

[0009] A cell detector based on electrical impedance, comprising: a conveying device, the conveying device comprising a conveyor belt and a screw slide, the conveyor belt is used to convey a test tube to a specified position, and the screw slide is used to control the lifting of the test tube; a filling device, the filling device comprising a filling host, a filling gun and a hydraulic telescopic cylinder, the filling host is installed on one side of the conveyor belt, the filling gun and the hydraulic telescopic cylinder are both installed in the filling host, the filling gun controls the telescopic length through the hydraulic telescopic cylinder, an infrared sensor is installed in the filling gun, and the infrared sensor is used to determine whether the test tube has reached the specified position; a detection device, the detection device comprises a filling mainframe, a filling gun and a hydraulic telescopic cylinder, the filling mainframe is installed on one side of the conveyor belt, the filling gun and the hydraulic telescopic cylinder are both installed in the filling mainframe, the filling gun controls the telescopic length through the hydraulic telescopic cylinder, an infrared sensor is installed in the filling gun, and the infrared sensor is used to determine whether the test tube has reached the specified position; The invention comprises a host computer and an impedance detection device, wherein the impedance detection device is connected to the host computer, and a metal needle is installed on the impedance detection device; a sorting device, wherein the sorting device comprises a rotating component and a grasping component, wherein the rotating component comprises a turntable, a rotating motor and a plurality of mechanical claws, wherein the turntable is connected to the rotating motor, and the plurality of mechanical claws are installed at the edge of the turntable, and the rotating motor is used to control the rotation of the turntable and the grasping of the plurality of mechanical claws, and the grasping component comprises an electric claw, a connecting block, a cylinder and a slide rail, wherein the cylinder is installed on the slide rail, one end of the cylinder is connected to the connecting block, and the lower end of the connecting block is fixedly connected to the electric claw.

[0010] In one embodiment of the utility model, there are two filling chambers in the filling host, and the two filling guns are used to fill water and 100% of the cell fluid to be tested respectively.

[0011] In one embodiment of the utility model, the screw slide includes a screw, a lifting block, a gripper and a drive motor, the lifting block is installed on the screw, the gripper is installed on the lifting block, and the drive motor is used to drive the lifting block to move up and down and the gripper to rotate left and right.

[0012] In one embodiment of the present invention, the electrical impedance detection device is a portable impedance detector.

[0013] In one embodiment of the present invention, the portable impedance detector is used for detecting single cells and cell populations, wherein the detection of the cell population includes analysis of cell proliferation, migration and apoptosis behaviors.

[0014] Beneficial effects of the utility model:

[0015] The utility model transmits the test tube to the designated position and drives the lifting of the screw slide table through the transmission device, and controls the filling gun to fill a certain concentration of cell fluid into the test tube for detection through the hydraulic telescopic cylinder installed in the filling main machine in the filling device, and detects the cell fluid of a certain concentration through the electrical impedance detection equipment and the host computer in the detection device, and finally sorts and grabs the detected cell fluid through the rotating component and the grabbing component in the sorting device, thereby, the morphology and structure of the cells can be observed, the efficiency of cell detection and the accuracy of analysis are improved, the operation is simple and the cost is low. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 This is a schematic diagram of the structure of a cell detector based on electrical impedance according to an embodiment of the utility model;

[0017] Figure 2 This is a schematic diagram of the structure of a conveyor belt according to an embodiment of the utility model;

[0018] Figure 3 This is a schematic structural diagram of a filling device according to an embodiment of the utility model;

[0019] Figure 4 This is a schematic diagram of the structure of a screw slide according to an embodiment of the utility model;

[0020] Figure 5 This is a schematic diagram of the relative positions of the metal needle and the screw slide in one embodiment of the utility model;

[0021] Figure 6 This is a schematic structural diagram of a rotating assembly according to an embodiment of the utility model;

[0022] Figure 7 This is a schematic structural diagram of a grabbing assembly according to an embodiment of the utility model. DETAILED DESCRIPTION

[0023] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0024] Figure 1 It is a schematic structural diagram of a cell detector based on electrical impedance according to an embodiment of the utility model.

[0025] like Figure 1As shown, the cell detector based on electrical impedance of the embodiment of the utility model comprises: a conveying device 10, a filling device 20, a detection device 300 and a sorting device 40, wherein the conveying device 10 comprises a conveyor belt 11 and a screw slide 12, the conveyor belt 11 is used to convey the test tube to the specified position, and the screw slide 12 is used to control the lifting of the test tube; the filling device 20 comprises a filling host 21, a filling gun 22 and a hydraulic telescopic cylinder 23, the filling host 21 is installed on one side of the conveyor belt 11, the filling gun 22 and the hydraulic telescopic cylinder 23 are both installed in the filling host 21, the filling gun 22 controls the telescopic length through the hydraulic telescopic cylinder 23, and an infrared sensor is installed in the filling gun 22, and the infrared sensor is used to determine whether the test tube has reached the specified position; the detection device 30 comprises a host computer 31 and an impedance detection device 32, the impedance detection device 32 is connected to the host computer 31, and a metal needle 321 is installed on the impedance detection device 32; the sorting device 40 includes a rotating component 41 and a grasping component 42, the rotating component 41 includes a turntable 411, a rotating motor 412 and a plurality of mechanical claws 413, the turntable 411 is connected to the rotating motor 412, and a plurality of mechanical claws 413 are installed on the edge of the turntable 411, the rotating motor 412 is used to control the rotation of the turntable 411 and the grasping of the plurality of mechanical claws 413, the grasping component 42 includes an electric claw 421, a connecting block 422, a cylinder 423 and a slide rail 424, the cylinder 423 is installed on the slide rail 424, one end of the cylinder 423 is connected to the connecting block 422, and the lower end of the connecting block 422 is fixedly connected to the electric claw 421.

[0026] like Figure 2 As shown, the conveyor belt 11 may be wrapped with a metal frame 13 on all sides, a gap may be opened on the upper part of the metal frame 13, and a plurality of cylindrical storage blocks 14 may be evenly distributed along the gap, wherein the top ends of the plurality of cylindrical storage blocks 14 may be opened with grooves for fixing the test tubes, and the bottom ends of the plurality of cylindrical storage blocks 14 are placed on the conveyor belt 11 and move with the conveyor belt 11.

[0027] like Figure 3 As shown, the laboratory staff places the test tube on the groove at the top of the cylindrical storage block 14. When the conveyor belt 11 conveys the test tube to the specified position, the built-in infrared sensor of the filling gun 22 senses the test tube. At this time, the conveyor belt 11 stops conveying, and the hydraulic telescopic cylinder 23 in the filling host 21 controls the filling gun 22 to retract and fill the cell fluid into the test tube. Among them, there are two filling guns 22 in the filling host 21. The two filling guns 22 can be used to fill water and 100% of the cell fluid to be tested in the test tube respectively. The staff can automatically mix the cell fluid according to the required concentration.

[0028] like Figure 4As shown, the screw slide 12 may include a screw 121, a lifting block 122, a gripper 123 and a drive motor 124, wherein the lifting block 122 is mounted on the screw 121, the gripper 123 is mounted on the lifting block 122, and the drive motor 124 is used to drive the lifting block 122 to move up and down and the gripper 123 to rotate left and right. After the test tube is filled with the cell solution to be tested by the filling gun 22, the drive motor 124 drives the lifting block 122 to move up and down to a height where the gripper 123 can grab the test tube, and controls the gripper 123 to rotate left and right to grab the test tube.

[0029] like Figure 5 As shown, when the gripper 123 moves to the position directly below the metal needle 321, the driving motor 124 drives the lifting block 122 to move upward to the metal needle 321 to contact the cell fluid to be detected. At this time, the impedance detection device 32 extracts the cell fluid to be detected through the metal needle 321, and analyzes the cell fluid to be detected through the host computer 31, wherein the impedance detection device 32 can be a portable impedance detector, which can be used not only for the detection of single cells, but also for the detection of cell groups, wherein the detection of cell groups can include the analysis of cell proliferation, migration and apoptosis behavior.

[0030] like Figure 6 As shown, the rotating assembly 41 may include a rotating disk 411, a rotating motor 412 and a plurality of mechanical claws 413. After the metal needle 321 extracts the cell fluid to be detected, the driving motor 124 controls the lifting block 122 to descend to a position where the mechanical claws 413 can grab the test tube. At this time, the rotating motor 412 controls the rotating disk 411 to rotate and controls any idle mechanical claw 413 to grab the test tube.

[0031] like Figure 7 As shown, the rotating motor 412 controls the rotation of the turntable 411. When the test tube grasped by the mechanical claw 413 is located below the grasping assembly 42, the turntable 411 stops rotating. At this time, the connecting block 422 can be moved forward, backward, left and right through the slide rail 424 so that the electric claw 421 can accurately grasp the test tube. The output end of the cylinder 423 is connected to the connecting block 422. When the electric claw 421 moves to a position where it can grasp the test tube, the cylinder 423 drives the connecting block 422 to extend and retract so that the electric claw 421 grasps the test tube, and after the electric claw 421 grasps the test tube, it moves left and right through the slide rail 424 to place the test tube in the test tube box.

[0032] According to the impedance-based cell detector of the embodiment of the utility model, the test tube is transported to the specified position and the screw slide is driven to rise and fall through the conveying device, and the filling gun is controlled by the hydraulic telescopic cylinder installed in the filling main machine in the filling device to fill a certain concentration of cell fluid into the test tube for detection, and the impedance detection equipment and the host computer in the detection device are used to detect the cell fluid of a certain concentration, and finally the rotating component and the grasping component in the sorting device are used to sort and grasp the detected cell fluid, thereby observing the morphology and structure of the cells, improving the efficiency of cell detection and the accuracy of analysis, and the operation is simple and the cost is low.

[0033] In the description of the present utility model, the terms "first" and "second" are used for descriptive purposes only and cannot be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include one or more of the features. The meaning of "plurality" is two or more, unless otherwise clearly and specifically defined.

[0034] In the present invention, unless otherwise clearly specified and limited, the terms "install", "connect", "connect", "fix" and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0035] In the present utility model, unless otherwise clearly specified and limited, a first feature being "above" or "below" a second feature may mean that the first and second features are in direct contact, or the first and second features are in indirect contact through an intermediate medium. Moreover, a first feature being "above", "above" or "above" a second feature may mean that the first feature is directly above or obliquely above the second feature, or simply means that the first feature is higher in level than the second feature. A first feature being "below", "below" or "below" a second feature may mean that the first feature is directly below or obliquely below the second feature, or simply means that the first feature is lower in level than the second feature.

[0036] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "example", "specific example", or "some examples" etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the utility model. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art may combine and combine the different embodiments or examples described in this specification and the features of the different embodiments or examples, without contradiction.

[0037] Although the embodiments of the present invention have been shown and described above, it can be understood that the above embodiments are exemplary and cannot be understood as limitations of the present invention. Ordinary technicians in the field can change, modify, replace and modify the above embodiments within the scope of the present invention.

Claims

1. A cell detector based on electrical impedance, characterized in that: include: A conveying device, the conveying device comprising a conveyor belt and a screw slide, the conveyor belt is used to convey the test tube to a specified position, and the screw slide is used to control the lifting of the test tube; A filling device, comprising a filling host, a filling gun and a hydraulic telescopic cylinder. The filling host is installed on one side of the conveyor belt, the filling gun and the hydraulic telescopic cylinder are both installed in the filling host, the filling gun controls the telescopic length through the hydraulic telescopic cylinder, and an infrared sensor is installed in the filling gun, and the infrared sensor is used to determine whether the test tube has reached the specified position; A detection device, the detection device comprising a host computer and an electrical impedance detection device, the electrical impedance detection device is connected to the host computer, and a metal needle is installed on the electrical impedance detection device; A sorting device, the sorting device includes a rotating component and a grasping component, the rotating component includes a turntable, a rotating motor and a plurality of mechanical claws, the turntable is connected to the rotating motor, the plurality of mechanical claws are installed at the edge of the turntable, the rotating motor is used to control the rotation of the turntable and the grasping of the plurality of mechanical claws, the grasping component includes an electric claw, a connecting block, a cylinder and a slide rail, the cylinder is installed on the slide rail, one end of the cylinder is connected to the connecting block, and the lower end of the connecting block is fixedly connected to the electric claw.

2. The cell detector based on electrical impedance according to claim 1, characterized in that: There are two filling guns in the filling host, and the two filling guns are used to fill water and 100% of the cell fluid to be tested respectively.

3. The cell detector based on electrical impedance according to claim 2, characterized in that: The screw slide comprises a screw, a lifting block, a gripper and a driving motor. The lifting block is mounted on the screw, the gripper is mounted on the lifting block, and the driving motor is used to drive the lifting block to move up and down and the gripper to rotate left and right.

4. The cell detector based on electrical impedance according to claim 3, characterized in that: The electrical impedance detection device is a portable impedance detector.

5. The cell detector based on electrical impedance according to claim 4, characterized in that: The portable impedance detector is used for detecting single cells and cell groups, wherein the detection of the cell group includes analysis of cell proliferation, migration and apoptosis behavior.

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