Multi-sample parallel bacterial culture device

Abstract

The utility model relates to the technical field of bacterial culture, in particular to a multi-sample parallel bacterial culture device, which comprises a base plate, a culture plate and a cover plate which are sequentially overlapped and combined, and further comprises a micropore channel assembly which comprises a plurality of micropore units arranged on the culture plate and channel units communicated with the micropore units, the micropore unit is used for inoculating a strain to be cultured, and the channel unit is used for introducing a culture solution; and the control supply assembly is arranged on the cover plate and is used for independently adjusting and controlling the strains inoculated in the micropore units. According to the utility model, through the microporous channel assembly arranged on the culture plate and the control supply assembly arranged on the cover plate, various strains inoculated and cultured in more than one microporous unit can be independently adjusted and controlled, so that a multi-sample parallel bacterial culture mode is realized; and a plurality of hole bottom supports distributed in a matrix on the substrate can be conveniently sterilized and cleaned.

Description

Technical Field

[0001] The utility model relates to the technical field of bacterial culture, in particular to a multi-sample parallel bacterial culture device. Background Art

[0002] Bacterial culture is the use of artificial methods in the laboratory to cultivate bacterial growth and reproduction. Its purpose is usually to use the cultured bacteria for research, identification and application.

[0003] At present, most of the culture devices in laboratories are only used for the specific culture of one type of bacteria. There are very few experimental culture devices with high versatility. When conducting controlled culture to change the bacterial species, the device usually needs to be reset. Moreover, the changing and controlling variables are not refined enough, and the impact of changes on the bacterial species cannot be monitored in time.

[0004] The above contents are only used to assist in understanding the technical solution of the present invention, and do not constitute an admission that the above contents are the closest prior art. Utility Model Content

[0005] The purpose of the utility model is to solve the above-mentioned shortcomings and provide a multi-sample parallel bacteria culture device.

[0006] In order to solve the above technical problems, the utility model adopts the following technical solution: a multi-sample parallel bacterial culture device, including a base plate, a culture plate and a cover plate stacked in sequence, and also includes:

[0007] A microporous channel assembly, comprising a plurality of microporous units arranged on the culture plate and a channel unit communicating with the microporous units, wherein the microporous units are used to inoculate the bacteria to be cultured, and the channel unit is used to pass the culture solution;

[0008] The control supply component is arranged on the cover plate and is used for independently regulating and controlling the bacterial species inoculated in the microporous unit.

[0009] Furthermore, the micropore unit includes a first micropore arranged on the culture plate, the first micropore is provided with openings on both the upper and lower sides, a second micropore is provided on the outer side of the first micropore, and a heating wire is provided on the second micropore.

[0010] Furthermore, the first micropore is connected to the second micropore, and the inner side of the first micropore and the outer side of the second micropore are connected to each other with a liquid inlet and a liquid outlet.

[0011] Furthermore, the channel unit includes a plurality of first flow channels arranged in a matrix on the culture plate and a plurality of second flow channels arranged equidistantly, one side of the first flow channel is connected to the liquid inlet port, and a plurality of parallel branches are arranged on the second flow channel, and the branches are connected to the liquid outlet port.

[0012] Furthermore, the control supply assembly includes a bracket arranged on the cover plate, the bracket is provided with a first through pipe and a second through pipe, and the first through pipe and the second through pipe are respectively provided with inlet pipes corresponding to the microporous units.

[0013] Furthermore, the control supply component also includes a control supply unit corresponding to the micropore unit and arranged on the bottom surface of the cover plate, and the control supply unit includes a functional board arranged on the cover plate, and the functional board is provided with a temperature probe, an ultrafine atomization nozzle and a mixed gas nozzle; the temperature probe is used to monitor and feedback adjust and control the temperature in each first micropore, the ultrafine atomization nozzle is used to control the humidity in each first micropore, and the mixed gas nozzle is used to adjust the gas composition introduced into each first micropore.

[0014] Furthermore, the control supply assembly also includes a plurality of droppers and spacers disposed on the cover plate, the plurality of droppers are respectively connected to the corresponding first flow channels, and the plurality of spacers are respectively used to block the corresponding liquid outlets.

[0015] Furthermore, a plurality of hole bottom supports arranged in a matrix are provided on the substrate; the hole bottom supports are used for the growth and attachment of bacteria.

[0016] Furthermore, limit transverse blocks are movably provided on both sides of the culture plate, and the adjacent end surfaces of the limit transverse blocks on both sides are connected to the base plate.

[0017] Compared with the prior art, the utility model has the following beneficial effects: the utility model can independently adjust and control the multiple bacterial species inoculated and cultured in more than one microporous unit through the cooperation of the microporous channel component arranged on the culture plate and the control supply component arranged on the cover plate, thereby realizing a multi-sample parallel bacterial culture method; and through the base plate combined on the lower side of the culture plate, by separating the base plate and the culture plate, the multiple hole bottom supports arranged in a matrix on the base plate can be conveniently sterilized and cleaned without affecting the next use. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] The drawings constituting part of the present application are used to provide a further understanding of the present invention. The exemplary embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute an improper limitation on the present invention. In the drawings:

[0019] Figure 1 A three-dimensional structural diagram of an embodiment of the utility model from a single perspective;

[0020] Figure 2 It is a three-dimensional structural diagram of an overall exploded perspective of an embodiment of the utility model;

[0021] Figure 3 A three-dimensional structural diagram of a culture plate in one embodiment of the utility model from one viewing angle;

[0022] Figure 4 for Figure 3 The structure diagram at A is enlarged;

[0023] Figure 5 A three-dimensional structural diagram of another perspective of an embodiment of the utility model;

[0024] Figure 6 for Figure 5 The structure diagram at B is enlarged;

[0025] Figure 7 This is a bottom structural diagram of a cover plate according to an embodiment of the utility model.

[0026] In the figure: 1. substrate; 2. culture plate; 3. cover plate; 4. microporous channel assembly; 41. first micropore; 42. second micropore; 43. liquid inlet port; 44. liquid outlet port; 45. first flow channel; 46. second flow channel; 5. control supply assembly; 51. bracket; 52. first through pipe; 53. second through pipe; 54. control supply unit; 541. functional board; 542. temperature probe; 543. ultrafine atomizing nozzle; 544. mixed gas nozzle; 55. dropper; 56. spacer; 6. bottom support; 7. limit horizontal block. DETAILED DESCRIPTION

[0027] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. In the absence of conflict, the embodiments in this application and the features in the embodiments can be combined with each other. Based on the embodiments in the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0028] It should be noted that if the embodiments of the present invention involve directional indications (such as up, down, left, right, front, back...), the directional indications are only used to explain the relative position relationship, movement status, etc. between the components in a certain specific posture. If the specific posture changes, the directional indications will also change accordingly.

[0029] In addition, if there are descriptions involving "first", "second", etc. in the embodiments of the utility model, the descriptions of "first", "second", etc. are only used for descriptive purposes and cannot be understood as indicating or implying their relative importance or implicitly indicating the number of technical features indicated. Therefore, the features defined as "first" and "second" may explicitly or implicitly include at least one of the features. In addition, the meaning of "and / or" appearing in the full text includes three parallel schemes. Taking "A and / or B" as an example, it includes scheme A, or scheme B, or schemes that satisfy both A and B. In addition, "multiple" refers to more than two. In addition, the technical solutions between the various embodiments can be combined with each other, but it must be based on the ability of ordinary technicians in this field to implement. When the combination of technical solutions is contradictory or cannot be implemented, it should be deemed that such a combination of technical solutions does not exist and is not within the scope of protection required by the utility model.

[0030] like Figure 1-7 As shown, the utility model is a multi-sample parallel bacterial culture device, comprising a base plate 1, a culture plate 2 and a cover plate 3 which are stacked and combined in sequence, and also includes:

[0031] The microporous channel assembly 4 comprises a plurality of microporous units arranged on the culture plate 2 and a channel unit communicating with the microporous units, wherein the microporous units are used to inoculate the bacteria to be cultured, and the channel unit is used to pass the culture solution;

[0032] The control supply component 5 is arranged on the cover plate 3, and is used to independently adjust and control the bacterial species inoculated in one or more microporous units included in the microporous channel component 4. With this design, through the one or more microporous channel components 4 arranged on the culture plate 2, multiple samples can be inoculated and cultured in parallel. At the same time, through the corresponding control supply components 5 in the one or more microporous units included in the microporous channel component 4, the bacterial species inoculated in each first micropore 41 can be independently controlled and adjusted, which has universality.

[0033] In one embodiment, the micropore unit includes a first micropore 41 disposed on the culture plate 2, the first micropore 41 is provided with an opening on both the upper and lower sides, a second micropore 42 is provided on the outer side of the first micropore 41, and a heating wire is provided on the second micropore 42. With this design, by forming a plurality of first micropores 41 integrally on the culture plate 2 and forming a second micropore 42 integrally outside the first micropore 41, bacterial inoculation can be conveniently and quickly performed inside the first micropore 41.

[0034] It should be noted that the diameter of the second microhole 42 is greater than that of the first microhole 41 , and the heating wire is designed inside the second microhole 42 and around the first microhole 41 , so that the heating wire can be used to provide heat around the first microhole 41 .

[0035] In one embodiment, the first micropore 41 is connected to the second micropore 42, and a liquid inlet 43 and a liquid outlet 44 are connected to the inner side of the first micropore 41 and the outer side of the second micropore 42. In this design, the nutrients around the bacteria in the first micropore 41 can be continuously renewed by opening the liquid inlet 43 and the liquid outlet 44 on the structure of the first micropore 41 and the second micropore 42 formed in one piece.

[0036] In one embodiment, the channel unit includes a plurality of first flow channels 45 arranged in a matrix and a plurality of second flow channels 46 arranged at equal intervals on the culture plate 2, one side of the first flow channel 45 is connected to the liquid inlet port 43, and a plurality of parallel branches are arranged on the second flow channel 46, and the branches are connected to the liquid outlet port 44. In this design, the nutrients in the first micropores 41 can be continuously updated by turning and slotting the plurality of first flow channels 45 and the plurality of second flow channels 46 on the culture plate 2 and connected to each first micropore 41, avoiding the limitation of the solid-liquid interface.

[0037] It should be noted that the inner groove bottom surface of the first flow channel 45 is designed to be an inclined structure to prevent the nutrients introduced into the first flow channel from flowing back.

[0038] In one embodiment, the control supply assembly 5 includes a bracket 51 disposed on the cover plate 3, and a first through pipe 52 and a second through pipe 53 are disposed on the bracket 51. The first through pipe 52 and the second through pipe 53 are respectively provided with inlet pipes corresponding to the micropore units. With this design, the bracket 51 on the cover plate 3 and the two pipelines designed on the bracket 51 can respectively deliver extremely fine water mist and mixed gas to the first micropores 41, thereby ensuring that the bacteria in the first micropores 41 are in a good growth and cultivation environment.

[0039] It should be noted that the inlet end of the first through-pipe 52 is designed with a humidifying system for use with it, and the inlet end of the second through-pipe 53 is designed with a mixed gas generator for use with it.

[0040] It should be noted that the corresponding inlet pipe on each microporous unit is designed with a corresponding control pump.

[0041] In one embodiment, the control supply assembly 5 also includes a control supply unit 54 corresponding to the micropore unit and arranged on the bottom surface of the cover plate 3. The control supply unit 54 includes a functional board 541 arranged on the cover plate 3, and the functional board 541 is provided with a temperature probe 542, an ultrafine atomizing nozzle 543 and a mixed gas nozzle 544; the temperature probe 542 is used to monitor the temperature in each first micropore 41 for feedback adjustment and control, the ultrafine atomizing nozzle 543 is used to control the humidity in each first micropore 41, and the mixed gas nozzle 544 is used to adjust the gas composition entering each first micropore 41. With this design, by turning the control supply unit 54 on the cover plate 3, the culture environment of the strain in each first micropore 41 can be independently controlled and adjusted to achieve the effect of independent environmental control.

[0042] It should be noted that the temperature probe 542 and the heating wire are electrically connected via a controller, and the temperature feedback monitored by the temperature probe 542 can be used to send a control signal to the controller to determine whether to start the heating wire to provide heat to the bacteria in the first micropore 41 .

[0043] In one embodiment, the control supply assembly 5 further includes a plurality of droppers 55 and spacers 56 disposed on the cover plate 3, wherein the plurality of droppers 55 are respectively connected to the corresponding first flow channels 45, and the plurality of spacers 56 are respectively used to block the corresponding liquid outlets 44. With this design, the droppers 55 corresponding to the first flow channels 45 are used to transport nutrients into the first micropores 41, thereby ensuring that each sample in the first micropores 41 obtains appropriate nutrients; the up and down movement of the spacers 56 can open and close the liquid outlets 44 penetrating the side of the first micropores 41, thereby continuously updating the subsequent nutrients.

[0044] In one embodiment, the substrate 1 is provided with a plurality of bottom supports 6 arranged in a matrix; the bottom supports 6 are used for bacterial growth and attachment. In this way, the bottom supports 6 designed on the substrate 1 are the same in number as the first micropores 41, and correspond one to one to provide a bottom support for bacterial inoculation.

[0045] It should be noted that the surface of the hole bottom support 6 is designed to be slightly convex, providing sufficient surface area and structural support, which is conducive to the growth and cultivation of bacteria.

[0046] It should be noted that the culture medium added to the well bottom support 6 is mixed with an enzyme labeling reagent, and the growth of bacteria can be indirectly reflected by measuring the turbidity in the liquid using an enzyme marker in the bacteria grown in the culture medium.

[0047] In one embodiment, the two sides of the culture plate 2 are movably provided with limit horizontal blocks 7, and the adjacent end faces of the limit horizontal blocks 7 on both sides are connected to the base plate 1. In this design, the base plate 1 superimposed on the lower end of the culture plate 2 can be limited by the limit horizontal blocks 7 hingedly connected by hinges on both sides of the bottom end of the culture plate 2, so that convenient assembly and disassembly are realized. After each experiment is completed, the bacteria on the surface of the well bottom support 6 arranged in a matrix on the separated base plate 1 can be cleaned and sterilized, which is conducive to the subsequent continued use.

[0048] It is obvious to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the present invention can be implemented in other specific forms without departing from the spirit or basic features of the present invention. Therefore, no matter from which point of view, the embodiments should be regarded as exemplary and non-restrictive, and the scope of the present invention is defined by the appended claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims are included in the present invention.

Claims

1. A multi-sample parallel bacterial culture device, comprising a base plate (1), a culture plate (2) and a cover plate (3) stacked in sequence, characterized in that: Also includes: A microporous channel assembly (4) comprising a plurality of microporous units arranged on the culture plate (2) and a channel unit communicating with the microporous units, wherein the microporous units are used to inoculate the bacteria to be cultured, and the channel unit is used to pass the culture solution; A control supply assembly (5) is arranged on the cover plate (3) and is used to independently regulate and control the bacterial species inoculated in the microporous unit.

2. The multi-sample parallel bacterial culture device according to claim 1, characterized in that: The micropore unit comprises a first micropore (41) arranged on a culture plate (2), the first micropore (41) is provided with openings on both upper and lower sides, a second micropore (42) is provided on the outer side of the first micropore (41), and a heating wire is provided on the second micropore (42).

3. The multi-sample parallel bacterial culture device according to claim 2, characterized in that: The first micropore (41) and the second micropore (42) are connected, and the inner side of the first micropore (41) and the outer side of the second micropore (42) are connected with a liquid inlet (43) and a liquid outlet (44).

4. The multi-sample parallel bacterial culture device according to claim 3, characterized in that: The channel unit comprises a plurality of first flow channels (45) arranged in a matrix and a plurality of second flow channels (46) arranged at equal intervals on the culture plate (2); one side of the first flow channel (45) is connected to the liquid inlet port (43); a plurality of parallel branches are arranged on the second flow channel (46), and the branches are connected to the liquid outlet port (44).

5. The multi-sample parallel bacterial culture device according to claim 1, characterized in that: The control supply assembly (5) comprises a bracket (51) arranged on the cover plate (3), the bracket (51) being provided with a first through pipe (52) and a second through pipe (53), the first through pipe (52) and the second through pipe (53) being provided with inlet pipes corresponding to the microporous units respectively.

6. The multi-sample parallel bacterial culture device according to claim 2, characterized in that: The control supply assembly (5) further comprises a control supply unit (54) corresponding to the micropore unit and arranged on the bottom surface of the cover plate (3); the control supply unit (54) comprises a functional board (541) arranged on the cover plate (3); the functional board (541) is provided with a temperature probe (542), an ultrafine atomizing nozzle (543) and a mixed gas nozzle (544); the temperature probe (542) is used for monitoring and feedback regulating the temperature in each first micropore (41); the ultrafine atomizing nozzle (543) is used for controlling the humidity in each first micropore (41); and the mixed gas nozzle (544) is used for adjusting the composition of the gas introduced into each first micropore (41).

7. The multi-sample parallel bacterial culture device according to claim 4, characterized in that: The control supply assembly (5) further comprises a plurality of droppers (55) and spacers (56) arranged on the cover plate (3); the plurality of droppers (55) are respectively connected to corresponding first flow channels (45); and the plurality of spacers (56) are respectively used to block corresponding liquid outlet ports (44).

8. The multi-sample parallel bacterial culture device according to claim 1, characterized in that: The substrate (1) is provided with a plurality of hole bottom supports (6) arranged and distributed in a matrix; the hole bottom supports (6) are used for bacterial growth and attachment.

9. The multi-sample parallel bacterial culture device according to claim 1, characterized in that: Limiting transverse blocks (7) are movably provided on both sides of the culture plate (2), and the adjacent end surfaces of the limiting transverse blocks (7) on both sides are connected to the base plate (1).

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