Electrochemiluminescence instant detector

Abstract

The utility model discloses an electrochemical luminescence instant detection instrument, relates to the field of electrochemical luminescence detection instruments, and solves the technical problem that the existing electrochemical luminescence detection instrument is inconvenient to place and take out a sample. The device comprises a shell, a power supply module, a camera and a micro-fluidic chip, wherein a mechanical access module is mounted at the bottom of the shell; the mechanical in-out module comprises a fixing plate, a chip platform and a rebound sliding rail, the fixing plate is fixedly installed at the bottom of the shell, the chip platform is installed on the fixing plate in a sliding mode, and the rebound sliding rail is fixedly installed at the end, away from the chip platform, of the fixing plate; the chip platform is connected with the movable end of the rebound sliding rail through a spring supporting rod, and a micro-fluidic chip is placed in the chip platform. The sample feeding and discharging efficiency can be improved, and waste of samples and consumption of reagents are reduced due to the stable feeding and discharging mode.

Description

Technical Field

[0001] The utility model relates to the field of electrochemical luminescence detection instruments, and more specifically, it relates to an electrochemical luminescence instant detection instrument. Background Art

[0002] Electrochemiluminescence detection technology is an analytical technology that combines electrochemistry and chemiluminescence. It generates luminescent excited state substances by inducing electrochemical reactions on the electrode surface. When these excited state substances return to the ground state, they release photons, thereby generating luminescence. By measuring the intensity of this luminescence, specific components in the sample can be quantitatively or qualitatively analyzed. Electrochemiluminescence detection technology is particularly suitable for the detection of biomarkers such as proteins and nucleic acids due to its advantages such as low background signals, high sensitivity and high selectivity.

[0003] However, traditional medical testing has defects such as long testing time, high equipment cost and relatively fixed testing sites, which greatly restricts the promotion of testing equipment and projects.

[0004] The existing electrochemiluminescence instant detector mainly includes a power module, a camera and a microfluidic chip. The microfluidic chip is filled with samples. The camera collects the luminescent signal on the microfluidic chip after the electrochemiluminescence test, and the collected data signal is transmitted back to the power module for processing, thereby realizing the electrochemiluminescence detection of the sample. Although the existing electrochemiluminescence detector can detect the sample instantly, the existing electrochemiluminescence detector is not convenient for placing and removing the sample. Utility Model Content

[0005] The technical problem to be solved by the utility model is to provide an electrochemiluminescence instant detector in view of the deficiencies of the prior art, so as to solve the technical problem that the prior electrochemiluminescence detector is not convenient for placing and taking out samples.

[0006] The utility model discloses an electrochemical luminescence instant detector, which comprises a housing, a power module, a camera and a microfluidic chip, wherein a mechanical inlet and outlet module is installed at the bottom of the housing;

[0007] The mechanical entry and exit module includes a fixed plate, a chip platform and a rebound slide track. The fixed plate is fixedly installed on the bottom of the shell, and the chip platform is slidably installed on the fixed plate. The rebound slide track is fixedly installed on the end of the fixed plate away from the chip platform. The chip platform is connected to the active end of the rebound slide track through a spring support rod. A microfluidic chip is placed on the chip platform. A spring connector for installing and positioning the microfluidic chip is fixedly installed on one side of the chip platform, and the camera is located above the microfluidic chip.

[0008] As a further improvement, the shell includes a front plate, a top plate and a bottom plate, one end of the top plate is fixedly connected to the front plate, the end of the front plate away from the top plate is fixedly connected to the bottom plate, the fixed plate is fixedly installed on the bottom plate, a light isolation plate is fixedly installed on the side of the front plate close to the camera, an observation hole is provided on the light isolation plate, a sleeve for protecting the camera is installed above the light isolation plate, and the camera is inserted in the sleeve.

[0009] Furthermore, a USB interface is fixedly installed on a side of the front plate away from the camera, the camera is electrically connected to an external terminal via the USB interface and a USB cable, and the power module is electrically connected to a spring connector via a wire.

[0010] Furthermore, the external terminal is a mobile phone.

[0011] Furthermore, an arc plate is fixedly connected to the middle part below the top plate, and one end of the arc plate away from the top plate is fixedly connected to the bottom plate, and a support plate is fixedly connected to the arc plate, and one end of the support plate away from the arc plate is fixedly connected to the top plate, a power module is installed on the support plate, and a handle is provided between the bottom of the support plate and the arc plate.

[0012] Furthermore, the power module includes a lithium battery charging management circuit, a main control chip circuit, a crystal oscillator circuit, a SWD debugging interface and a main control chip startup circuit, a reset circuit, a single-chip microcomputer power supply circuit, a USB interface / lithium battery circuit and a Buck-Boost circuit. The lithium battery charging management circuit, the crystal oscillator circuit, the SWD debugging interface and the main control chip startup circuit, the reset circuit, the single-chip microcomputer power supply circuit, the USB interface / lithium battery circuit and the Buck-Boost circuit are all electrically connected to the main control chip circuit.

[0013] Furthermore, the handle is coated with anti-slip material.

[0014] Furthermore, the chip platform is provided with two threaded holes, and the spring connector is fixedly mounted on the chip platform by two hexagonal screws installed in the threaded holes.

[0015] Furthermore, a microfluidic chip is placed on the chip platform, the electrodes on the microfluidic chip are electrically connected to the spring connector, and the light-emitting area on the microfluidic chip is sent under the observation hole on the light-isolating plate through a mechanical entry and exit module.

[0016] Furthermore, the top plate is provided with a voltage adjustment knob, a digital display and a start switch, and the voltage adjustment knob, the digital display and the start switch are all electrically connected to the power module.

[0017] Furthermore, a slide groove is provided on the fixing plate, a slider is slidably installed in the slide groove, and the bottom of the chip platform is fixedly connected to the slider.

[0018] Beneficial Effects

[0019] The advantages of the utility model are:

[0020] The utility model provides a power module and a mechanical entry and exit module, and realizes a mechanical entry and exit method of a push-type ejection microfluidic chip through the mechanical entry and exit module, thereby improving the entry and exit efficiency of samples. The stable entry and exit method also reduces the consumption of samples and reagents, saving costs. At the same time, a handle is provided to facilitate the carrying of the detector. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 It is a schematic diagram of the overall structure of the instant detector in the utility model;

[0022] Figure 2 This is a structural diagram of the mechanical entry and exit module in the utility model;

[0023] Figure 3 This is a lithium battery charging management circuit diagram in the power module of the utility model;

[0024] Figure 4 This is the circuit diagram of the main control chip in the power module of the utility model;

[0025] Figure 5 This is a crystal oscillator circuit diagram in the power module of the utility model;

[0026] Figure 6 This is a SWD debugging interface and startup circuit diagram in the power module of the utility model;

[0027] Figure 7 This is a reset circuit diagram in the power module of the utility model;

[0028] Figure 8 This is a power supply circuit diagram of a single-chip microcomputer in a power supply module of the utility model;

[0029] Fig. 9 This is the USB interface / lithium battery circuit diagram in the power module of the utility model;

[0030] Fig.10 This is a Buck-Boost circuit diagram in the power module of the utility model;

[0031] Fig.11 This is a schematic diagram of the front side of the PCB of the power module in the utility model;

[0032] Fig.12 This is a schematic diagram of the back side of the PCB of the power module in the utility model;

[0033] Fig.13 It is a curve diagram showing the result of detecting H2O2 in the luminol system by the closed bipolar electrode electrochemiluminescence microfluidic chip A in the utility model;

[0034] Fig.14 This is a curve diagram of the results of detecting luteinizing hormone using the dry immune closed electrode electrochemiluminescence microfluidic chip B in the Ru(II) system in the utility model.

[0035] Among them: 1-shell, 2-top plate, 3-bottom plate, 4-mechanical entry and exit module, 5-chip platform, 6-fixed plate, 7-rebound slide rail, 8-spring support rod, 9-microfluidic chip, 10-spring connector, 11-slide groove, 12-front plate, 13-light isolation plate, 14-sleeve, 15-camera, 16-arc plate, 17-support plate, 18-power module, 19-handle, 20-hexagonal screw, 21-voltage adjustment knob, 22-digital display, 23-start switch, 24-USB interface, 25-external terminal, 26-lithium battery charging management circuit, 27-main control chip circuit, 28-crystal oscillator circuit, 29-SWD debugging interface and main control chip startup circuit, 30-reset circuit, 31-single-chip computer power supply circuit, 32-USB interface / lithium battery circuit, 33-Buck-Boost circuit. DETAILED DESCRIPTION

[0036] The utility model is further described below in conjunction with the embodiments, but does not constitute any limitation to the utility model. Any limited modifications made by anyone within the scope of the claims of the utility model are still within the scope of the claims of the utility model.

[0037] See also Figure 1-Figure 14 The utility model discloses an electrochemical luminescence instant detector, which comprises a housing 1, a power module 18, a camera 15 and a microfluidic chip 9. A mechanical inlet and outlet module 4 is installed at the bottom of the housing 1.

[0038] The mechanical entry and exit module 4 includes a fixed plate 6, a chip platform 5 and a rebound slide rail 7. The fixed plate 6 is fixedly installed at the bottom of the housing 1. The chip platform 5 is slidably installed on the fixed plate 6. The rebound slide rail 7 is fixedly installed at one end of the fixed plate 6 away from the chip platform 5. The chip platform 5 is connected to the active end of the rebound slide rail 7 through a spring support rod 8. A microfluidic chip 9 is placed in the chip platform 5. A spring connector 10 for installing and positioning the microfluidic chip 9 is fixedly installed on one side of the chip platform 5. The camera 15 is located above the microfluidic chip 9. The microfluidic chip 9 is filled with samples to be tested.

[0039] When the chip platform 5 does not need to be fixed, the spring of the spring support rod 8 is in a naturally stretched state, and the chip platform 5 is not pulled by the rebound slide rail 7 .

[0040] When the microfluidic chip 9 of the chip platform 5 needs to be fixed, the chip platform 5 is pressed to move the chip platform 5 toward the rebound slide track 7. The rebound slide track 7 causes the spring of the spring support rod 8 to be in a contracted state, and the chip platform 5 is pulled and fixed by the spring of the spring support rod 8.

[0041] When the microfluidic chip 9 on the chip platform 5 needs to be taken out, the chip platform 5 is pressed again to move the chip platform 5 toward the rebound slide track 7. The rebound slide track 7 restores the spring of the spring support rod 8 to its original naturally extended state. The chip platform 5 is moved away from the rebound slide track 7 by the elastic force of the spring support rod 8, making it convenient to take out the microfluidic chip 9.

[0042] The housing 1 includes a front plate 12, a top plate 2 and a bottom plate 3, one end of the top plate 2 is fixedly connected to the front plate 12, one end of the front plate 12 away from the top plate 2 is fixedly connected to the bottom plate 3, the fixing plate 6 is fixedly mounted on the bottom plate 3, a light-isolating plate 13 is fixedly mounted on the side of the front plate 12 close to the camera 15, the light-isolating plate 13 is provided with an observation hole, a sleeve 14 for protecting the camera 15 is installed above the light-isolating plate 13, and the camera 15 is inserted in the sleeve 14. The sleeve 14 can be used to fix the camera 15 and can also be used to protect the camera 15 to prevent it from being damaged.

[0043] A USB interface 24 is fixedly installed on one side of the front panel 12 away from the camera 15, and the camera 15 is electrically connected to the external terminal 25 through the USB interface 24 and the USB line. The power module 18 is electrically connected to the spring connector 10 through a wire. The external terminal 25 is a mobile phone. The mobile phone communicates with the power module 18 through the USB interface, and the mobile phone is used for electrochemiluminescence imaging preview, calculating electrochemiluminescence values, displaying imaging data processing results and storing data.

[0044] An arc plate 16 is fixedly connected to the middle part below the top plate 2, and one end of the arc plate 16 away from the top plate 2 is fixedly connected to the bottom plate 3. A support plate 17 is fixedly connected to the arc plate 16, and one end of the support plate 17 away from the arc plate 16 is fixedly connected to the top plate 2. A power module 18 is installed on the support plate 17, and a handle 19 is provided between the bottom of the support plate 17 and the arc plate 16. The handle 19 is coated with anti-slip material to increase friction so that the detector can be lifted more stably.

[0045] The chip platform 5 is provided with two threaded holes, and the spring connector 10 is fixedly mounted on the chip platform 5 by two hexagonal screws 20 installed in the threaded holes.

[0046] A microfluidic chip 9 is placed on the chip platform 5 , the electrodes on the microfluidic chip 9 are electrically connected to the spring connector 10 , and the light-emitting area on the microfluidic chip 9 is sent under the observation hole on the light-isolating plate 13 through the mechanical entry and exit module 4 .

[0047] The top plate 2 is provided with a voltage adjustment knob 21, a digital display 22 and a start switch 23, and the voltage adjustment knob 21, the digital display 22 and the start switch 23 are all electrically connected to the power module 18. The digital display 22 is used to display the voltage value, the start switch 23 is used to start the power module 18, and the voltage adjustment knob 21 is used to change the output voltage of the power module 18.

[0048] The fixing plate 6 is provided with a slide groove 11, in which a slider is slidably mounted, and the bottom of the chip platform 5 is fixedly connected to the slider. The chip platform 5 slides through the slider, thereby extending the sliding distance.

[0049] The power module 18 is used to supply power to the electrical equipment in the housing 1 .

[0050] The power module 18 includes a lithium battery charging management circuit, a main control chip circuit, a crystal oscillator circuit, a SWD debugging interface and a startup circuit, a reset circuit, a single-chip power supply circuit, a USB interface circuit, a lithium battery circuit, and a Buck-Boost circuit. The lithium battery charging management circuit 26, the crystal oscillator circuit 28, the SWD debugging interface and the main control chip startup circuit 29, the reset circuit 30, the single-chip power supply circuit 31, the USB interface / lithium battery circuit 32, and the Buck-Boost circuit 33 are all electrically connected to the main control chip circuit 27.

[0051] Lithium battery charging management circuit diagram Figure 3As shown, this circuit uses the BQ24295 power management chip. The VBUS pin is connected to the charging interface, and the flyback voltage generated when the charging end is unplugged is filtered through a small-capacitance decoupling capacitor. The D+ and D- pins are connected to the USB module circuit, and the STAT pin is connected in series to the main control chip through a protection resistor and an LED light to display the charging status or fault prompt; the SDA and SCL pins are used as the data line and clock line of I2C communication, and use open-drain output and a 10KΩ pull-up resistor to prevent short circuits when multiple devices are used and to implement an arbitration mechanism; the INT pin is connected to the main control chip through a pull-up resistor to transmit charging status and fault signals; the function of the OTG pin needs to be controlled by the main control chip program to enable or disable the boost mode; the CE pin is connected to the ground through a 10KΩ pull-down resistor to enable the charging function; the ILIM pin is connected to the ground wire with a resistor to set the charging input current range; the TS pin is connected to a negative temperature Thermistor with a temperature coefficient of 0.18V is used to monitor the battery temperature and report the status to the main control chip through the INT pin; the QON pin remains floating to avoid using the BAT field effect transistor control mode; the BAT pin is directly connected to the positive electrode of the rechargeable battery, and the battery ripple voltage is filtered through a 10μF capacitor; the SYS pin ensures the transmission of the normal operation signal of the system; the PGND pin grounds the input and output capacitors in the circuit; the SW pin is connected to the output inductor and decoupling capacitor, and is connected to the BTST pin through a bootstrap capacitor to control the high-voltage MOS tube; the BTST pin operates by controlling the conduction of the high-side MOS tube; the REGN pin is connected to the decoupling capacitor, and the noise interference is reduced through the bias circuit of the TS pin; the PMID pin is used to output the boosted voltage, which is filtered through a parallel capacitor, and the output voltage status is judged by the LED light and the protection resistor.

[0052] The main control chip circuit diagram is as follows Figure 4 As shown in the figure, this circuit uses the STM32F103C8T6 chip. The figure shows the connection names of each pin of the chip.

[0053] Crystal oscillator circuit diagram Figure 5 As shown, the circuit uses crystal oscillators with frequencies of 8MHz and 32.768kHz, and two capacitors with equal capacitance are connected in parallel to ensure the stability of the oscillation circuit.

[0054] SWD debugging interface and startup circuit diagram as shown Figure 6As shown in the figure, the SWD debug interface is a serial single-wire interface for program debugging. It can read and write the registers and memory of the microcontroller through SWDIO and SWCLK. SWDIO is used for bidirectional data transmission, and SWCLK is used to maintain clock synchronization. Through this interface, program debugging, optimization and troubleshooting can be performed quickly and efficiently. The main control chip startup circuit uses the main flash memory, and the corresponding pins BOOT0 and BOOT1 are both low.

[0055] Reset circuit diagram Figure 7 As shown, this circuit uses a low-level reset, and the parallel capacitor is used to eliminate key jitter.

[0056] The microcontroller power supply circuit diagram is as follows Figure 8 As shown in the figure, the power supply voltage of the microcontroller is about 3.3V. It needs an LDO circuit to convert the 5V voltage output by the lithium battery charging management chip BQ24295 into 3.3V, which can ensure the normal operation of the microcontroller. The LDO circuit uses the AMS1117 chip.

[0057] USB interface / lithium battery circuit diagram Fig. 9 As shown, the USB uses a Micro-B female connector, where the D+ and D- pins are connected to the corresponding pins of the BQ24295 chip, which is used for the chip to detect the input source. The detection process is divided into three steps: data contact detection, main detection, and non-standard adapter detection. The battery model used in this circuit is a single-cell 18650 lithium battery with a battery capacity of 1500mAh, which is soldered to the circuit board with a battery box.

[0058] Buck-Boost circuit diagram Fig.10 , the XL6008E1 chip is used as a buck-boost converter, with an input voltage range of 3.6V to 32V, supporting a lower range of input voltage, and an adjustable range that meets the voltage required by the instrument (5V to 30V). LED lights and parallel capacitors of large-capacitance capacitors and small-capacitance capacitors are added to the input and output interfaces, and the pin FB is connected to the sliding resistor. The stable operating point of the circuit should always meet the FB pin voltage of 1.25V, and the stability of the output voltage is guaranteed by feedback closed-loop control.

[0059] With respect to the above-mentioned electrochemical luminescence instant detector, this article illustrates the technical effects of the mechanical in / out module 4 and the power module 18 on the experiment through the following two application methods:

[0060] Application method 1: Detecting hydrogen peroxide (H2O2) under the luminol system, the microfluidic chip 9 adopts a closed bipolar electrode electrochemiluminescence microfluidic chip A.

[0061] (1) 12 μL of the prepared mixed solution (4 mM luminol solution and H2O2 solution of different concentrations mixed in equal volumes) was dripped into the reporter channel of the closed bipolar electrode electrochemiluminescent microfluidic chip A by a pipette, and then 12 μL of 10× phosphate buffer (PBS) was dripped into the support channel of the closed bipolar electrode electrochemiluminescent microfluidic chip A to connect the conductive channel of the microfluidic chip A.

[0062] (2) Press the chip platform 5 to make it pop out, place the microfluidic chip A on the chip platform 5 and electrically connect it to the spring connector 10, then press the chip platform 5 again, and send the light-emitting area on the microfluidic chip A to the bottom of the observation hole on the light-isolating plate 13 through the mechanical entry and exit module 4.

[0063] (3) Connect the external terminal 25 (mobile phone) to the USB interface 24 via a USB data cable, open the video acquisition interface of the existing detection software on the mobile phone, set the white balance to 2800K and the exposure time to 127ms, and click the video recording button after debugging. At the same time, turn on the start switch 23 to provide an excitation voltage of 7.5V for the microfluidic chip A to stimulate the electrochemical luminescence reaction of luminol and H2O2.

[0064] (4) After the reaction is completed, click the video recording button on the mobile phone again to end the recording, and then open the video analysis interface of the detection software to perform data analysis on the recorded video. The data and pictures of the analysis results are displayed on the interface.

[0065] (5) Origin software was used to analyze the six sets of data and make a standard curve evaluation.

[0066] The H2O2 concentrations in the mixed solution of this experiment were set to: 0mM, 1mM, 2mM, 5mM, 7.5mM and 10mM. The experimental results are shown in Fig.13 As shown in the results, it can be seen that with the increase of H2O2 concentration, the electrochemical luminescence intensity value also increases accordingly; and there is a good linear relationship between H2O2 concentration and electrochemical luminescence intensity value, the linear fitting equation is Y=0.6550X+0.0015, and the correlation coefficient R2 is 0.9971 (5 times repeated). Therefore, this detector can detect H2O2 under the luminol system.

[0067] Application method 2: The target used in the immune experiment is luteinizing hormone (LH), and the microfluidic chip 9 uses a dry immune closed bipolar electrode electrochemiluminescent microfluidic chip B.

[0068] (1) The sample pad was pretreated. The specific process was as follows: the glass fiber was first soaked in a sample pad treatment solution (0.05% PVP, 0.025% casein and 0.25% S19 surfactant dissolved in 10×PBS buffer (pH 7.2)), and then baked in a forced air drying oven for 1 hour.

[0069] (2) Pre-treating the conjugate pad: first, immerse the glass fiber in 10×PBS buffer (pH 7.2) containing 0.25% S19, then bake in a forced air drying oven for 1 h, then use a film drawing instrument to draw the labeled antibody solution on the pre-treated glass fiber, and bake at 37°C for 1 h.

[0070] (3) Pre-treating the detection pad, the specific process is: using a film stripper to stripe the capture antibody on the detection pad (NC film), and baking at 37° C. for 2 h.

[0071] (4) Assemble the treated sample loading pad, conjugate pad, detection pad and electrode sheet, drip 35 μL of sample solution on the sample loading pad, carry out immune reaction for 3.5 min, then add 30 μL of 10× PBS buffer to rinse on the sample loading pad for 3 min, and drip 30 μL of 10× PBS buffer on the connection pad to connect the conductive channel of microfluidic chip B.

[0072] (5) Press the chip platform 5 to make it pop out, place the microfluidic chip B on the chip platform 5 and electrically connect it to the spring connector 10, then press the chip platform 5 again, and send the light-emitting area on the microfluidic chip B to the bottom of the observation hole on the light-isolating plate 13 through the mechanical entry and exit module 4.

[0073] (6) Connect the external terminal 25 (mobile phone) to the USB interface 24 via a USB data cable, open the video acquisition interface of the existing detection software on the mobile phone, set the white balance to 2800K and the exposure time to 127ms, and click the video recording button after debugging. At the same time, turn on the start switch 23 to provide an excitation voltage of 11V for the chip to stimulate the electrochemical luminescence reaction based on Ru(II) and the intramolecular co-reaction reagent (lysine).

[0074] (7) After the reaction is completed, click the video recording button on the mobile phone again to end the recording, and then open the video analysis interface of the detection software to perform data analysis on the recorded video. The data and pictures of the analysis results are displayed on the interface.

[0075] (8) Origin software was used to analyze the three sets of data and make a standard curve evaluation.

[0076] The concentrations of luteinizing hormone (LH) in the mixed solution of this experiment were set to: 1mIU / mL, 5mIU / mL and 22mIU / mL. The experimental results are as follows Fig.14As shown. From the results, it can be seen that with the use of this detector, as the LH concentration increases, the ratio of the electrochemiluminescence intensity value of the detection area (i.e., T area) on the chip to the electrochemiluminescence intensity value of the control area (i.e., C area) (i.e., T / C) also increases accordingly; and there is a good linear relationship between the logarithm of the LH concentration and T / C, the linear fitting equation is Y=2.040X+2.318, and the correlation coefficient R2 is 0.9889 (5 times repeated). Therefore, this detector can be used for immunodetection of LH under the Ru(II) system.

[0077] The above is only a preferred embodiment of the present invention. It should be pointed out that for those skilled in the art, several modifications and improvements can be made without departing from the structure of the present invention, which will not affect the effect of the implementation of the present invention and the practicality of the patent.

Claims

1. An electrochemical luminescence instant detector, comprising a housing (1), a power module (18), a camera (15) and a microfluidic chip (9), characterized in that: A mechanical entry and exit module (4) is installed at the bottom of the housing (1); The mechanical entry and exit module (4) comprises a fixed plate (6), a chip platform (5) and a rebound slide rail (7); the fixed plate (6) is fixedly mounted on the bottom of the housing (1); the chip platform (5) is slidably mounted on the fixed plate (6); the rebound slide rail (7) is fixedly mounted on one end of the fixed plate (6) away from the chip platform (5); the chip platform (5) is connected to the movable end of the rebound slide rail (7) via a spring support rod (8); a microfluidic chip (9) is placed on the chip platform (5); a spring connector (10) for mounting and positioning the microfluidic chip (9) is fixedly mounted on one side of the chip platform (5); and the camera (15) is located above the microfluidic chip (9).

2. An electrochemiluminescence instant detector according to claim 1, characterized in that: The housing (1) comprises a front plate (12), a top plate (2) and a bottom plate (3); one end of the top plate (2) is fixedly connected to the front plate (12); one end of the front plate (12) away from the top plate (2) is fixedly connected to the bottom plate (3); the fixing plate (6) is fixedly mounted on the bottom plate (3); a light isolation plate (13) is fixedly mounted on a side of the front plate (12) close to the camera (15); an observation hole is provided on the light isolation plate (13); a sleeve (14) for protecting the camera (15) is mounted above the light isolation plate (13); and the camera (15) is plugged into the sleeve (14).

3. An electrochemiluminescence instant detector according to claim 2, characterized in that: A USB interface (24) is fixedly mounted on a side of the front panel (12) away from the camera (15); the camera (15) is electrically connected to an external terminal (25) via the USB interface (24) and a USB line; and the power module (18) is electrically connected to the spring connector (10) via a wire.

4. An electrochemiluminescence instant detector according to claim 3, characterized in that: The external terminal (25) is a mobile phone.

5. The electrochemiluminescence instant detector according to claim 1, characterized in that: An arc-shaped plate (16) is fixedly connected to the middle part below the top plate (2); one end of the arc-shaped plate (16) away from the top plate (2) is fixedly connected to the bottom plate (3); a support plate (17) is fixedly connected to the arc-shaped plate (16); one end of the support plate (17) away from the arc-shaped plate (16) is fixedly connected to the top plate (2); a power module (18) is installed on the support plate (17); and a handle (19) is provided between the bottom of the support plate (17) and the arc-shaped plate (16).

6. An electrochemiluminescence instant detector according to claim 5, characterized in that: The power module (18) comprises a lithium battery charging management circuit (26), a main control chip circuit (27), a crystal oscillator circuit (28), a SWD debugging interface and a main control chip startup circuit (29), a reset circuit (30), a single-chip computer power supply circuit (31), a USB interface / lithium battery circuit (32) and a Buck-Boost circuit (33); the lithium battery charging management circuit (26), the crystal oscillator circuit (28), the SWD debugging interface and a main control chip startup circuit (29), the reset circuit (30), the single-chip computer power supply circuit (31), the USB interface / lithium battery circuit (32) and the Buck-Boost circuit (33) are all electrically connected to the main control chip circuit (27).

7. An electrochemiluminescence instant detector according to claim 5, characterized in that: The handle (19) is coated with anti-slip material.

8. An electrochemiluminescence instant detector according to claim 1, characterized in that: The chip platform (5) is provided with two threaded holes, and the spring connector (10) is fixedly mounted on the chip platform (5) via two hexagonal screws (20) mounted in the threaded holes.

9. The electrochemiluminescence instant detector according to claim 1, characterized in that: The top plate (2) is provided with a voltage adjustment knob (21), a digital display (22) and a start switch (23), and the voltage adjustment knob (21), the digital display (22) and the start switch (23) are all electrically connected to the power module (18).

10. The electrochemiluminescence instant detector according to claim 1, characterized in that: The fixing plate (6) is provided with a slide groove (11), a slider is slidably installed in the slide groove (11), and the bottom of the chip platform (5) is fixedly connected to the slider.

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