Biological sample processing device

Abstract

The utility model discloses a biological sample treatment device. The biological sample treatment device comprises a sample container, a cover plate and a first driving device, the sample container is provided with a plurality of sample containing cavities for containing samples, each sample containing cavity is provided with an opening, and the first driving device is used for moving the cover plate so that the cover plate can move between a first position and a second position. When the cover plate is located at the first position, the cover plate covers and presses the opening of the sample accommodating cavity; when the cover plate is located at the second position, the cover plate leaves the opening of the sample containing cavity, and the cover plate is a paper plate. According to the scheme, the sample accommodating cavity is covered by the paper cover plate, so that liquid volatilized in the sample accommodating cavity is absorbed by the paper cover plate and is not easy to volatilize into the adjacent sample accommodating cavity, and the extracted nucleic acid is prevented from cross contamination. In addition, the biological sample treatment device further comprises a reaction reagent adding device, so that various reaction reagents can be automatically added in the sample treatment process, and the whole-process automation of biological sample extraction is realized.

Description

Technical Field

[0001] The present application relates to the technical field of biochemical detection, mainly to a biological sample processing device. Background Art

[0002] Nucleic acid extractors are devices that extract and purify nucleic acids from biological samples. They mainly extract nucleic acids from a variety of biological samples such as whole blood, viruses, tissues, plants, bacteria, and cultured cells. During the extraction process, biological samples often need to be heated. However, when the biological sample liquid is heated, it is easy to cause the biological sample liquid to volatilize. During the volatilization process of the biological sample liquid, it may evaporate into the adjacent biological sample liquid, causing the sample to be contaminated.

[0003] In some relatively complex nucleic acid extraction processes, such as methylation detection, various reaction reagents need to be added multiple times in the middle. Existing nucleic acid extraction instruments are either too expensive to be popularized or cannot be fully automated, requiring users to manually add reaction reagents in the middle. Utility Model Content

[0004] In view of the above-mentioned deficiencies or shortcomings, the utility model provides a fully automatic biological sample processing device and solves the problem of mutual contamination caused by volatilization of biological sample liquid.

[0005] The present disclosure provides a biological sample processing device, comprising a sample container, a cover plate and a first driving device, wherein the sample container is provided with a sample cavity, and the sample cavity is provided with an opening for adding a sample, and the first driving device is used to move the cover plate so that the cover plate moves between a first position and a second position, and when the cover plate is located at the first position, the cover plate is pressed against the opening of the sample cavity to seal the opening; when the cover plate is located at the second position, the cover plate leaves the opening of the sample cavity, wherein the cover plate comprises a paper board and / or a foam board.

[0006] Optionally, the sample container is provided with a plurality of the sample cavities, each of the sample cavities is provided with the opening, and when the cover is located at the first position, the cover is pressed against the openings of the plurality of the sample cavities to seal the openings of the plurality of the sample cavities.

[0007] Optionally, a heating device is also included, and the heating device is used to heat the sample container.

[0008] Optionally, it further includes a first reaction reagent container and a magnetic rod assembly, wherein the magnetic rod assembly includes a magnetic rod and a magnetic rod sleeve, and the magnetic rod is used to magnetically attract magnetic beads in the sample in the sample container.

[0009] Optionally, the magnetic bar assembly further includes a second driving device and a third driving device, the second driving device is used to drive the magnetic bar to move, and the third driving device is used to drive the magnetic bar sleeve to move.

[0010] Optionally, a reaction reagent adding device is further included, wherein the reaction reagent adding device includes a second reaction reagent container and a pump body, and the pump body is used to pump the reaction reagent from the second reaction reagent container to the first reaction reagent container.

[0011] Optionally, the reaction reagent adding device also includes a fourth driving device and a connector, wherein the connector is connected to the pump body, and the fourth driving device can drive the connector to move between a fifth position and a sixth position; when the connector is in the fifth position, the connector is connected to the second reaction reagent container, so that the pump body, the connector and the second reaction reagent container are connected; when the connector is in the sixth position, the connector is not connected to the second reaction reagent container.

[0012] Optionally, it also includes a droplet detection sensor and a fifth driving device, wherein the fifth driving device drives the reaction reagent adding device to move between a third position and a fourth position, and when the reaction reagent adding device is in the third position, the pump body can pump the reaction reagent from the second reaction reagent container to the first reaction reagent container, and when the reaction reagent adding device is in the fourth position, the pump body can pump the reaction reagent from the second reaction reagent container to the droplet detection sensor.

[0013] Optionally, a sixth driving device is further included, the sample container and the first reaction reagent container are fixed to each other, and the sixth driving device is used to drive the sample container and the first reaction reagent container to move.

[0014] Optionally, the first reaction reagent container includes a plurality of first reaction reagent cavities.

[0015] The above solution covers the opening of the sample cavity by a cover plate, so that the volatilized liquid in the sample cavity is absorbed by the cover plate and is not easily volatilized to the adjacent sample container to cause sample contamination, thereby ensuring the purity of the extracted nucleic acid. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1-3 Schematic diagrams of the structure of the biological sample processing device of the present disclosure at different angles.

[0017] Figure 4 A schematic diagram of a partial structure of a biological sample processing device.

[0018] Figure 5This is a schematic diagram of the structure of the reaction reagent adding device.

[0019] Figure 6 for Figure 5 A schematic diagram of the structure of the reaction reagent adding device in another angle and another state. The symbols in the figure are: 100, sample container; 110, heating device; 120, sample chamber; 200, cover plate; 300, first driving device; 410, first reaction reagent container; 411, first reaction reagent chamber; 420, magnetic rod assembly; 421, magnetic rod; 422, second driving device; 423, magnetic rod sleeve; 424, third driving device; 500, reaction reagent adding device; 510, second reaction reagent container; 520, pump body; 530, fourth driving device; 540, moving block; 550, connector; 560, valve; 600, droplet detection sensor; 700, fifth driving device. DETAILED DESCRIPTION

[0020] In order to make the purpose, technical solution and advantages of the present application more clearly understood, the present application is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present application and are not used to limit the present application.

[0021] It should be noted that the illustrations provided in this embodiment are only used to illustrate the basic concept of the utility model in a schematic manner. Therefore, the illustrations only show the components related to the utility model rather than being drawn according to the number, shape and size of the components in actual implementation. In actual implementation, the type, quantity and proportion of each component may be changed arbitrarily, and the component layout type may also be more complicated.

[0022] The structures, proportions, sizes, etc. illustrated in the drawings of this specification are only used to match the contents disclosed in the specification so as to facilitate understanding and reading by persons familiar with this technology. They are not used to limit the conditions under which the present invention can be implemented, and therefore have no substantive technical significance. Any structural modification, change in proportion or adjustment of size, without affecting the effects and purposes that can be achieved by the present invention, should still fall within the scope of the technical contents disclosed by the present invention.

[0023] The directions or positional relationships indicated by the terms "upper", "lower", "left", "right", "middle", "longitudinal", "lateral", "horizontal", "inner", "outer", "radial", "circumferential" and the like in this specification are based on the directions or positional relationships shown in the drawings and are only for the convenience of simplifying the description. They do not indicate or imply that the devices or elements referred to must have a specific direction, be constructed and operate in a specific direction, and therefore cannot be understood as limiting the present invention. In addition, the terms "first" and "second" are used for descriptive purposes only and cannot be understood as indicating or implying relative importance.

[0024] The present disclosure provides a biological sample processing device, such as Figure 1-4 As shown, the sample container 100, the cover plate 200 and the first driving device 300 are included. The sample container 100 is provided with a sample cavity 120. The sample cavity 120 is provided with an opening, and the opening is mainly used for placing the sample in the sample cavity 120. The first driving device 300 is used to move the cover plate 200 so that the cover plate 200 moves between a first position and a second position. When the cover plate 200 is located at the first position, the cover plate 200 covers the opening of the sample cavity 120. When the cover plate 200 is located at the second position, the cover plate 200 leaves the opening of the sample cavity 120.

[0025] Specifically, if Figure 4 As shown, the sample container 100 is provided with a plurality of sample cavities 120, each of which is provided with an opening to accommodate the sample. When the cover plate 200 is located at the first position, the cover plate 200 covers the openings of the plurality of sample cavities 120. More specifically, the first driving device 300 drives the cover plate 200 to move in the up-down direction. The horizontal height of the first position is lower than that of the second position. That is, when the cover plate 200 needs to be pressed on the opening of the sample cavity 120, the first driving device 300 needs to move the cover plate 200 downward; when the cover plate 200 is no longer needed to be pressed on the opening of the sample cavity, the first driving device 300 moves the cover plate 200 upward so that the cover plate 200 is no longer attached to the opening of the sample cavity. It should be understood that when the cover plate 200 covers the opening of the sample cavity 120, the opening will be sealed so that the liquid in the sample cavity 120 cannot evaporate from the opening.

[0026] It should be noted that, since the sample needs to be extracted and purified at a certain temperature, in some embodiments, the biological sample processing device further includes a heating device 110 , and the heating device 110 is used to heat the sample container 100 .

[0027] In addition, the cover plate 200 is a paper plate and / or a foam plate. Specifically, the cover plate 200 is composed of absorbent tissue paper, double-sided adhesive, and EVA foam.

[0028] Optionally, the biological sample processing device further includes a first reaction reagent container 410 and a magnetic rod assembly 420, wherein the magnetic rod assembly 420 includes a magnetic rod 421 and a magnetic rod sleeve 423, wherein the magnetic rod 421 is used to magnetically attract magnetic beads in the sample in the sample cavity 120. Specifically, the magnetic rod assembly 420 further includes a second driving device 422 and a third driving device 424, wherein the second driving device 422 is used to drive the magnetic rod 421 to move, and the third driving device is used to drive the magnetic rod sleeve 423 to move. Optionally, the biological sample processing device further includes a sixth driving device, wherein the sample container 100 and the first reaction reagent container 410 are fixed to each other, and the sixth driving device is used to drive the sample container 100 and the first reaction reagent container 410 to move.

[0029] Under normal circumstances, the magnetic bar 421 is located above the first reaction reagent container 410. Then, driven by the sixth driving device, the sample container moves to the bottom of the magnetic bar 421 and the magnetic bar cover 423. Then, the magnetic bar 421 and the magnetic bar cover 423 are driven downward by the second driving device 422 and the third driving device 424 respectively, so that the magnetic bar 421 can absorb the magnetic beads in the sample container. At this time, since the magnetic bar cover 423 is set outside the magnetic bar 421, the magnetic beads cannot contact the magnetic bar 421, but are closely attached to the magnetic bar cover 423. After absorbing the magnetic beads, the magnetic bar 421 and the magnetic bar cover 423 are driven upward by the second driving device 422 and the third driving device 424 respectively, so that the magnetic beads are separated from the sample container. Then, the first reaction reagent container 410 is driven by the sixth driving device to move to the bottom of the magnetic bar 421 and the magnetic bar cover 423. Then, the magnetic bar 421 and the magnetic bar cover 423 are driven downward by the second driving device 422 and the third driving device 424 respectively to allow the magnetic beads to enter the first reaction reagent container 410. The magnetic bar 421 is then driven upward by the second driving device 422, while the magnetic bar cover does not move, so that the magnetic beads are separated from the magnetic bar 421 and lose the magnetic attraction, and fall off from the outer surface of the magnetic bar cover into the sample. Finally, the magnetic bar cover 423 is driven upward by the third driving device 424.

[0030] In some embodiments, the first reaction reagent container 410 includes a plurality of first reaction reagent cavities 411, and different first reaction reagent cavities 411 can be used to place different reaction reagents. Therefore, in some embodiments, the magnetic rod 421 and the magnetic rod sleeve 423 can be controlled to enter different first reaction reagent cavities 411 with the magnetic beads, or to enter different first reaction reagent cavities 411 in sequence, and to be mixed with different reaction reagents in sequence. In addition, the same reaction reagent can be placed in the plurality of first reaction reagent cavities 411 of the first reaction reagent container 410, and the present disclosure does not make specific restrictions. In addition, the first reaction reagent container 410 can be a 96-deep well plate, provided with 96 first reaction reagent cavities 411.

[0031] Optionally, the biological sample processing device of the present disclosure further includes a reaction reagent adding device 500. Figure 5 and 6 As shown, the reaction reagent adding device 500 includes a second reaction reagent container 510 and a pump body 520, and the pump body 520 is used to pump the reaction reagent from the second reaction reagent container 510 to the first reaction reagent container 410. Specifically, the reaction reagent adding device 500 also includes a valve 560, and the valve 560 is located between the second reaction reagent container 510 and the pump body 520. Optionally, the reaction reagent adding device 500 includes a plurality of valves 560, and the second reaction reagent container 510 is formed with a plurality of second reaction reagent cavities, and a plurality of second reaction reagent cavities can be placed with different reaction reagents. And optionally, each valve 560 corresponds to each second reaction reagent cavity one by one. The pump body is communicated with each valve 560 through the first pipeline, and each valve 560 is communicated with the corresponding reaction reagent cavity through the second pipeline. Therefore, when all valves 560 are opened, the pump body can pump gas to each second reaction reagent cavity after starting to pump out the reaction reagent in each second reaction reagent cavity. If it is desired that only the reaction reagent in one of the second reaction reagent cavities be pumped out, the valve 560 corresponding to the second reaction reagent cavity can be opened, and the other valves 560 can be closed at the same time, so that the gas output by the pump body is only delivered to the second reaction reagent cavity. In addition, the above scheme can also control the amount of reaction reagent flowing out of the second reaction reagent container 510 by different pumping pressures of the pump body 520 and / or the opening time of the valve 560, so that the structure of the present disclosure can be adapted to the nucleic acid extraction of different samples.

[0032] Optionally, the reaction reagent adding device 500 also includes a fourth driving device 530, a moving block 540 and a plurality of connectors 550. Wherein, each second pipeline corresponds to each connector 550 one by one. Corresponding to the passage of each valve, the pump body, the valve, the second pipeline and the connector 550 connected to the second pipeline are connected in sequence, so that the gas of the pump body can pass through the valve, the second pipeline and the connector 550 in sequence and pump out from the connector 550. The connector 550 is arranged on the moving block 540, and the fourth driving device 530 is used to drive the moving block 540 to drive the connector to move, so that the connector 550 can move between the fifth position and the sixth position. When the connector 550 is in the fifth position, the connector 550 is communicated with the second reaction reagent container 510, so that the gas of the pump body 520 can pass through the valve, the second pipeline and the connector 550 in sequence to be transported to the second reaction reagent chamber of the second reaction reagent container 510. When the connector 550 is in the sixth position, the connector 550 is not connected to the second reaction reagent container 510, and the gas in the pump body 520 cannot be delivered to the second reaction reagent chamber. Since the second reaction reagent needs to be replaced, the second reaction reagent container 510 needs to be replaced frequently. If the pipeline is directly connected to the second reaction reagent container, it is likely that the pipeline needs to be disassembled and assembled multiple times each time the second reaction reagent container 510 is replaced, making the operation complicated and the air tightness of the pipeline needs to be re-tested. The above scheme drives the connector to achieve the connection between the pump body and the second reaction reagent container 510 through the fourth driving device 530, so that when the second reaction reagent container 510 is replaced, the second pipeline and the second reaction reagent container 510 can be separated by raising the connector. After the second reaction reagent container 510 is installed, the connector is lowered to connect the second reaction reagent container 510, so that the second pipeline and the second reaction reagent container 510 can be connected, so that the second reaction reagent container 510 can be easily disassembled and assembled without the need to perform air tightness test on the pipeline.

[0033] The biological sample processing device optionally further includes a droplet detection sensor 600 and a fifth drive device 700, the fifth drive device 700 drives the reaction reagent adding device 500 to move between the third position and the fourth position, when the reaction reagent adding device 500 is in the third position, the pump body 520 can pump the reaction reagent from the second reaction reagent container 510 to the first reaction reagent container 410, when the reaction reagent adding device 500 is in the fourth position, the pump body 520 can pump the reaction reagent from the second reaction reagent container 510 to the droplet detection sensor 600. Specifically, the fifth drive device 700 can drive the reaction reagent adding device 500 to move between the third position and the fourth position in the left and right direction. When the reaction reagent adding device 500 is in the third position, as described above, the pump body 520 can pump the reaction reagent from the second reaction reagent container 510 to the first reaction reagent container 410 located below the reaction reagent adding device 500. It should be noted that the first reaction reagent container 410 at this time has been moved to the bottom of the reaction reagent adding device 500 under the drive of the sixth drive device.

[0034] When the reaction reagent adding device 500 is in the fourth position, the pump body 520 can pump the reaction reagent from the second reaction reagent container 510 to the droplet detection sensor 600, and detect whether there is liquid outflow through the droplet detection sensor 600. After determining that there is continuous and stable outflow of liquid (in some embodiments, the droplet detection sensor 600 detects liquid for 10 seconds, which can be considered as continuous and stable outflow of liquid), it is ensured that the reaction reagent fills the pipeline, thereby ensuring that the subsequent liquid addition amount to the first reaction reagent container 410 remains stable and is not prone to deviation.

[0035] During use, first, the sample is placed in the sample chamber 120, and the sample is heated by the heating device 110. At this time, since the cover plate 200 has covered the sample chamber, the sample in the sample chamber is prevented from volatilizing to other sample chambers to cause cross contamination. At the same time, the nucleic acid molecules in the sample will bind to the magnetic beads, so that the magnetic beads are loaded with the nucleic acid molecules of the sample.

[0036] Then, the sixth driving device drives the sample container 100 to move to the bottom of the magnetic rod 421, and absorbs the magnetic beads through the magnetic rod assembly 420. After absorbing the magnetic beads and lifting the magnetic rod 421 and the magnetic rod sleeve 423, the sixth driving device drives the first reaction reagent container 410 to move to the bottom of the magnetic rod 421 and the magnetic rod sleeve 423, and controls the second driving device 422 and the third driving device 424 to separate the magnetic rod 421 from the magnetic beads, and the magnetic beads fall into the first reaction reagent cavity. The magnetic beads transferred to the first reaction reagent container 410 are further transferred between its various cavities by the magnetic rod and the magnetic rod sleeve.

[0037] Secondly, the reaction reagent adding device 500 moves to the fourth position under the drive of the fifth driving device 700, and the droplet detection is performed by the droplet detection sensor 600 to ensure that the reaction reagent adding device 500 can continuously and stably sample. The fifth driving device 700 drives the reaction reagent adding device 500 to move to the third position, and the sixth driving device drives the first reaction reagent container 410 to move to the bottom of the reaction reagent adding device 500. The pump body 520 starts to pump the reaction reagent from the second reaction reagent container 510 to the first reaction reagent container 410. If other reagents still need to be added, it is necessary to control the fifth driving device 700 and the reaction reagent adding device 500 to add different reagents to the first reaction reagent cavity.

[0038] In the above scheme, the first driving device 300, the second driving device 422, the third driving device 424, the fourth driving device 530, the fifth driving device 700 and the sixth driving device are used to realize the automatic extraction of samples, so that it can be completed without manual work, so as to facilitate large-scale nucleic acid extraction and detection of samples. The technical features of the above embodiments can be combined arbitrarily. In order to make the description concise, all possible combinations of the technical features in the above embodiments are not described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0039] The above-mentioned embodiments only express several implementation methods of the present application, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the scope of the utility model patent. It should be pointed out that, for a person of ordinary skill in the art, several variations and improvements can be made without departing from the concept of the present application, and these all belong to the protection scope of the present application. Therefore, the protection scope of the patent of the present application shall be subject to the attached claims.

Claims

1. A biological sample processing device, characterized in that: The invention comprises a sample container, a cover plate and a first driving device, wherein the sample container is provided with a sample cavity, and the sample cavity is provided with an opening for adding a sample, and the first driving device is used to move the cover plate so that the cover plate moves between a first position and a second position, and when the cover plate is located at the first position, the cover plate is pressed against the opening of the sample cavity to seal the opening; when the cover plate is located at the second position, the cover plate leaves the opening of the sample cavity, wherein the cover plate comprises a paper board and / or a foam board.

2. The biological sample processing device according to claim 1, characterized in that: The sample container is provided with a plurality of the sample cavities, each of which is provided with the opening. When the cover is located at the first position, the cover is pressed against the openings of the plurality of the sample cavities to seal the openings of the plurality of the sample cavities.

3. The biological sample processing device according to claim 1, characterized in that: Also included is a heating device, which is used to heat the sample container.

4. The biological sample processing device according to claim 1, characterized in that: It also includes a first reaction reagent container and a magnetic rod assembly. The magnetic rod assembly includes a magnetic rod and a magnetic rod sleeve. The magnetic rod is used to magnetically attract magnetic beads in the sample in the sample container.

5. The biological sample processing device according to claim 4, characterized in that: The magnetic bar assembly further comprises a second driving device and a third driving device, wherein the second driving device is used for driving the magnetic bar to move, and the third driving device is used for driving the magnetic bar sleeve to move.

6. The biological sample processing device according to claim 4, characterized in that: It also includes a reaction reagent adding device, which includes a second reaction reagent container and a pump body, and the pump body is used to pump the reaction reagent from the second reaction reagent container to the first reaction reagent container.

7. The biological sample processing device according to claim 6, characterized in that: The reaction reagent adding device also includes a fourth driving device and a connecting head, wherein the connecting head is connected to the pump body, and the fourth driving device can drive the connecting head to move between a fifth position and a sixth position; when the connecting head is in the fifth position, the connecting head is connected to the second reaction reagent container, so that the pump body, the connecting head and the second reaction reagent container are connected; when the connecting head is in the sixth position, the connecting head is not connected to the second reaction reagent container.

8. The biological sample processing device according to claim 6, characterized in that: It also includes a droplet detection sensor and a fifth driving device, wherein the fifth driving device drives the reaction reagent adding device to move between a third position and a fourth position. When the reaction reagent adding device is in the third position, the pump body can pump the reaction reagent from the second reaction reagent container to the first reaction reagent container. When the reaction reagent adding device is in the fourth position, the pump body can pump the reaction reagent from the second reaction reagent container to the droplet detection sensor.

9. The biological sample processing device according to claim 4, characterized in that: It also includes a sixth driving device, the sample container and the first reaction reagent container are fixed to each other, and the sixth driving device is used to drive the sample container and the first reaction reagent container to move.

10. The biological sample processing device according to any one of claims 4 to 9, characterized in that: The first reaction reagent container includes a plurality of first reaction reagent cavities.

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